• Fermentation of cellulosic biomass goes through an atypical glycolytic pathway in this thermophilic bacterium, with various glycolytic enzymes capable of utilizing different phosphate donors, including GTP and inorganic pyrophosphate (PP i ), in addition to or in place of the usual ATP. (biomedcentral.com)
  • From the six HXKs analyzed here, only ZmHXK9 has minimal hexose phosphorylating activity even though enzymatic function of all isoforms (ZmHXK4-9) was confirmed using a yeast complementation approach. (biomedcentral.com)
  • Despite the detailed in vitro characterization of the enzymatic properties of yeast Sac1p, the exact cellular function of this protein has remained obscure. (embl.de)
  • PEPCKs have been generally classified according to their nucleotide specificities: enzymes from bacteria, yeast, and plants mainly use adenosine nucleotides, but enzymes from a variety of eukaryotes and mammals use guanosine or inosine phosphates ( 24 ). (asm.org)
  • The depletion of DOM over time was strongly correlated with increases in expression of many genes associated with heterotrophy (e.g., amino acid, fatty acid, and carbohydrate metabolism) belonging to a Hydrogenophaga strain that accounted for a relatively large percentage (~8%) of the metatranscriptome. (frontiersin.org)
  • Carbohydrate metabolism, inborn errors refer to genetic disorders that affect the body's ability to properly process carbohydrates, leading to various metabolic disorders. (lookformedical.com)
  • Lactic acid bacteria (LAB) may improve microbial status in the intestines and increase the metabolic activity towards polyphenolic degradation. (biomedcentral.com)
  • Comparison of real-time PCR and transcriptomics results showed that the trends of gene expression in glycolytic, carbon metabolism and glutathione metabolic pathways were identical. (biomedcentral.com)
  • High purity Arabinobiose (syrup) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Through in vitro antifungal assays screening the compound with efficacy, transcriptomics and proteomics were performed to investigate the pharmacological mechanisms of the effective compound against Trichophyton mentagrophytes . (biomedcentral.com)
  • In vitro antifungal assays showed that rosmarinic acid had a favorable inhibitory effect on fungi. (biomedcentral.com)
  • Hexokinase (HXK) is a glycolytic regulatory enzyme that catalyzes the irreversible phosphorylation reaction of D-hexoses at the sixth carbon using ATP-Mg 2+ as a phosphate donor. (biomedcentral.com)
  • The transcriptomic findings exhibited that the differentially expressed genes of fungus after rosmarinic acid intervention were mainly enriched in the carbon metabolism pathway, while the proteomic findings suggested that rosmarinic acid could inhibit the average growth of Trichophyton mentagrophytes by interfering with the expression of enolase in the glycolysis pathway. (biomedcentral.com)
  • This reaction is the first step in the gluconeogenic pathway, in which citric acid cycle intermediates are converted to hexose. (asm.org)
  • These factors include sugar transporters and invertases as well as metabolites, including sucrose and glucose, which function as signalling molecules to modulate gene expression. (sun.ac.za)
  • A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (lookformedical.com)
  • We discuss the historical relevance and the benefits of pulque consumption, its chemical and nutritional properties, including the health benefits associated with diverse lactic acid bacteria with probiotic potential isolated from the beverage. (frontiersin.org)
  • For instance, the transformation of plants with a yeast-derived invertase targeted to different subcellular compartments has led to the elucidation of several key aspects of sugar metabolism, including phloem loading mechanisms, the regulation of photosynthesis by sugars, the importance of sugar-metabolism compartmentation with regards to sucrose biosynthesis, storage and distribution, as well as the role of cell-wall invertase in phloem unloading and sink strength. (sun.ac.za)
  • The production of hexose 6 phosphate is a key node in different pathways that are required for a successful germination. (biomedcentral.com)
  • The genetic transformation of plants and the subsequent development of transgenic lines with disturbed sugar metabolism have made an unprecedented impact on the study of sugar translocation and -partitioning. (sun.ac.za)
  • The pulse of acetogenesis appears to have been collectively catalyzed by a number of different organisms and metabolisms, most prominently pyruvate:ferredoxin oxidoreductase. (frontiersin.org)
  • as well as the highest solubilization percentage of minimally pretreated mid-season switchgrass in comparison to other organisms[ 7 ], while fermenting the derived sugars to ethanol and acetate plus hydrogen (H 2 ) [ 8 ]. (biomedcentral.com)
  • The compound occurs in the majority of aerobic organisms, from bacteria to higher plants and animals. (doctorinternet.com)
  • Purple nonsulfur bacteria (PNSB) are metabolically versatile organisms which are able to grow either aerobically in the dark or anaerobically in the light using hydrogen or organic compounds as electron donors and subsequently fixing CO 2 ( 16 ). (asm.org)
  • Transgenic lines overproducing the yeast-derived invertase in either the vacuole (Vac-Inv) or apoplast (Apo-Inv) were utilised to analyse the effect of the altered sugar levels in sink and source organs on the expression of sugar transporters, as well as the endogenous cell wall invertase and inhibitors in these plants. (sun.ac.za)
  • Pathogens that attack plants promote massive reprogramming of the plant metabolism for the synthesis of chemical defenses-a process that can be costly in terms of plant growth and fitness [ 10 ]. (biomedcentral.com)
  • Therefore, the dramatic changes of sugar type and concentration during the germination process undoubtedly regulates the enzyme activity and the pattern of gene expression of several enzymes [ 5 , 6 ]. (biomedcentral.com)
  • Le génome de C. phytofermentans code pour 171 enzymes qui dégradent les polysaccharides (CAZymes), soulignant l'ensemble complexe d'enzymes nécessaires pour transformer la biomasse en sucres. (cea.fr)
  • This is the first study to explore the inhibitory effects of compounds from P. frutescens on Trichophyton mentagrophytes and its mechanism of action coupled with the antifungal activity in vitro from network pharmacology, transcriptomics and proteomics. (biomedcentral.com)
  • A comprehensive analysis of the dynamic changes in primary metabolism was performed, which included whole transcriptome analysis, nontargeted proteomics, and photosynthetic activity measurements in potato cv. (biomedcentral.com)
  • Transcript levels of the sucrose transporter NtSUT1 and hexose transporter NtMST1 encoding genes increased significantly in the source leaves and roots of Vac-Inv lines, whereas increased NtMst1 transcript levels were also detected in the roots of Apo-Inv lines. (sun.ac.za)
  • In: Sulfur Metabolism in Higher Plants - Fundamental, Environmental and Agricultural Aspects Springer International Publishing AG. (rothamsted.ac.uk)
  • Our work demonstrates the existence of a potential xanthan gum food chain involving at least two members of different phyla of gut bacteria and provides an initial framework for understanding how widespread consumption of a recently introduced food additive influences human microbiomes. (nature.com)
  • To deter- mine the effect of the G609D mutation on Cphy3925 enzyme activity, we purified WT and ET versions of the enzyme (Fig. 3A) and tested their in vitro catalysis of the two-step, bidirectional reactions converting acetyl-CoA to ethanol using either NADH or NADPH cofactors. (researchgate.net)
  • These transgenic lines displayed varying increases in invertase activity, altered sugar levels and consequently disturbed sink-source interactions and sugar partitioning. (sun.ac.za)
  • The key findings of the present study manifested that rosmarinic acid, a medicinal compound extracted from P. frutescens , had pharmacological activity in inhibiting the growth of Trichophyton mentagrophytes by affecting its enolase expression to reduce metabolism. (biomedcentral.com)
  • We focused on the dynamics of the primary metabolism-related processes during PVY NTN infection. (biomedcentral.com)
  • How bacteria talk to each other: regulation of gene expression by quorum sensing. (microbiologyresearch.org)
  • In this study, a similar strategy of transgenic plant analysis was employed to expand our insight into the regulation of sugar partitioning. (sun.ac.za)
  • The factors governing sugar partitioning co-ordinate its distribution in response to intrinsic and environmental signals. (sun.ac.za)
  • Fucose is a monosaccharide that is commonly found in the cell walls of bacteria and is involved in the formation of glycoproteins and glycolipids. (lookformedical.com)
  • Transformation was assessed by the standard parameters of morphological alteration, increased hexose uptake, loss of density inhibition, and anchorage-independent growth. (embl.de)
  • Seed germination is a crucial process in the plant life cycle when a dramatic variation of type and sugar content occurs just as the seed is hydrated. (biomedcentral.com)
  • The presence of all epitopes can be differentially modulated in vitro using glycoside hydrolase family 43 and family 51 arabinofuranosidases. (megazyme.com)
  • Here we show that the ability to digest xanthan gum is common in human gut microbiomes from industrialized countries and appears contingent on a single uncultured bacterium in the family Ruminococcaceae. (nature.com)