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OocytesCumulus CellsIn Vitro Oocyte Maturation TechniquesOogenesisFertilization in VitroEmbryo Culture TechniquesBlastocystOvarian FollicleMeiosisEmbryonic DevelopmentOocyte RetrievalEctogenesisParthenogenesisCattleFollicular FluidCryopreservationFollicle Stimulating HormoneCells, CulturedCulture MediaCleavage Stage, OvumEmbryo TransferMetaphaseOvarySwineChorionic GonadotropinSperm Injections, IntracytoplasmicSperm MaturationFertilizationMorulaCell Culture TechniquesEmbryonic and Fetal DevelopmentContraceptives, Oral, CombinedVitrificationGranulosa CellsPregnancyCysteamineGrowth Differentiation Factor 9HorsesSexual MaturationCell NucleusIncubatorsZona PellucidaCell DifferentiationSperm-Ovum InteractionsMicroscopy, UltravioletEthylene GlycolGonadotropinsOvulationCytoplasmProgesteroneMaturation-Promoting Factorbeta-N-Acetyl-GalactosaminidaseEmbryo, MammalianReproductive Techniques, AssistedCulture TechniquesZygoteSuperovulationTissue and Organ HarvestingLuteinizing HormoneBone Morphogenetic Protein 15Gonadotropins, EquineLive BirthOvulation InductionEpidermal Growth FactorRNA, MessengerFetal ViabilityTime FactorsSus scrofaMilrinonePolycystic Ovary SyndromeCoculture TechniquesTissue Culture TechniquesOocyte DonationMercaptoethanolReceptors, FSHHypoxanthineEstradiolGap JunctionsCell CommunicationCulture Media, Serum-FreeMice, Inbred C57BLFallopian TubesGene Expression Regulation, DevelopmentalMicroscopy, ConfocalNuclear Transfer TechniquesInhibinsGlutathioneReverse Transcriptase Polymerase Chain ReactionCell Nucleus ShapeGoatsFertilityPregnancy RateOvumSerum Albumin, BovineSpindle ApparatusDendritic CellsGene Expression RegulationModels, AnimalSpermatozoaCell Count
OocytesOocyte maturation rateEmbryosEmbryo developmentMelatoninBovineEmbryonic developmentFertilizationInhibitionChromosomesSpermProductionMediumStudyEffectsMatureQualityResultsActivationNormalRemainsOocytes in vitroDenuded porcine oocytesFollicular fluidExposure of porcineOocyte growthCumulus expansionEmbryo productionComplexesVivoExpanded cumulus cellsDevelopmentalNuclearOvariesBlastocystMammalianMorphologicalMeioticInhibitsResumptionConcentrationsCharacteristicsProteinActivityProduceRoleControlReliable
Oocytes17
  • We also employed the CK2-specific inhibitor CX-4945 to validate the effect of CK2 activity on the polar body extrusion, spindle assembly, microtubule stability and DNA damage level in porcine oocytes. (biomedcentral.com)
  • The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
  • Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
  • Conclusions: This study compared the efficiencies of resveratrol, melatonin and their combination for protecting porcine oocytes from heat stress. (sagepub.com)
  • The disruption of the levels of steroid hormones Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and caused the mature failure of in vitro cultured oocytes. (sagepub.com)
  • FF-MAS increases the quality of in vitro oocyte maturation, and thus the developmental potential of oocytes exposed to FF-MAS during in vitro maturation is improved. (cambridge.org)
  • in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
  • Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
  • The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
  • Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
  • To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
  • Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
  • Oocytes were collected from aspirated porcine tertiary follicles. (biomedcentral.com)
  • This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)
  • To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
  • Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
  • Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
Oocyte maturation rate3
  • This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)
  • Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
  • Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)
Embryos5
  • This study evaluated the final output and quality of in vitro produced blastocysts derived from in vivo recovered sheep embryos cultured at various early developmental stages to blastocyst. (cambridge.org)
  • Current results confirm the temporal sensitivities of sheep embryos to in vitro culture, regardless of the culture conditions. (cambridge.org)
  • Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
  • ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
  • Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
Embryo development3
  • Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
  • Scientists attempting to discover the basic requirements for successful embryo development have a hurdle when using in vitro embryo manufacturing strategies (2). (vetmedmosul.com)
  • ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
Melatonin4
  • It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
  • Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)
  • The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
  • For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
Bovine1
  • It could be concluded that the presence of urea at 40 mg/dL in maturation medium reduced the percentage of bovine oocyte maturation in vitro. (sciencegate.app)
Embryonic development1
Fertilization1
Inhibition1
  • Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)
Chromosomes1
  • Immunostaining and immunoblotting analysis showed that CK2 was constantly expressed and located on the chromosomes during the entire oocyte meiotic maturation. (biomedcentral.com)
Sperm1
  • Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)
Production2
  • The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
  • As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)
Medium3
  • In the current study, we utilized modified Spirulina maxima pectin nanoparticles as a supplement to improve porcine in vitro maturation medium. (mdpi.com)
  • In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
  • Each petri dish contained three drops of maturation medium (300 µl/drops) according to the groups. (sciencegate.app)
Study2
  • In the current study, we explored the functional roles of CK2 on the porcine oocyte meiotic maturation. (biomedcentral.com)
  • In the current study, we investigated the beneficial effects of resveratrol on in vitro porcine oocyte maturation under heat stress (HS). (sagepub.com)
Effects1
  • HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
Mature1
  • Relative abundance of mature MIR21 was quantified at 0, 8, 16, 24, 32, and 42 h of in vitro (IVM) with or without treatment with 3-isobutyl-1-methylxanthine (IBMX). (biomedcentral.com)
Quality1
  • A critical issue for their mone secretion was caused by the abnormal expression of fertility failure is the poor quality of oocyte maturation. (sagepub.com)
Results1
  • Our results indicate MIR21 is active and important during meiotic maturation of the oocyte. (biomedcentral.com)
Activation1
  • CK2β directly binds and inhibits Mos to prevent premature MAPK activation and oocyte maturation [ 22 ]. (biomedcentral.com)
Normal1
  • Our findings demonstrate that CK2 promotes the porcine oocyte maturation by ensuring normal spindle assembly and DNA damage repair. (biomedcentral.com)
Remains1
Oocytes in vitro1
  • This work is the first to describe the negative effects of pergafast 201 on the maturation of porcine oocytes in vitro, confirming the negative effect of pergafast on the course of meiotic maturation and on the ability of the oocyte to reach the final stage of maturation, as well as its effect on the increased incidence of abnormal dividing spindles. (nusl.cz)
Denuded porcine oocytes1
  • Yamauchi, N & Nagai, T 1999, ' Male pronuclear formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine ', Biology of reproduction , vol. 61, no. 3, pp. 828-833. (elsevierpure.com)
Follicular fluid2
Exposure of porcine2
  • The aim of this work was to evaluate the effect of pergafast 201 on selected specific markers of meiotic maturation after exposure of porcine oocytes to pergafast 201 in low concentrations in vitro. (nusl.cz)
  • 11. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro. (nih.gov)
Oocyte growth2
  • 3. The epidermal growth factor network: role in oocyte growth, maturation and developmental competence. (nih.gov)
  • The mammalian cumulus-oocyte complex (COCs) promotes oocyte growth and development duringlong stages of folliculogenesis and oogenesis. (biolifesas.org)
Cumulus expansion2
  • After culture, the degree of cumulus expansion and frequency of nuclear maturation were determined. (embrapa.br)
  • 5. Knockdown of TrkA in cumulus oocyte complexes (COCs) inhibits EGF-induced cumulus expansion by down-regulation of IL-6. (nih.gov)
Embryo production2
  • This is the first in vitro embryo production attempt in sei and Bryde's whales. (cambridge.org)
  • The traditional use of FSH for superovulation has limitations, including labor requirements and variability in superovulation response, affecting the success of in vivo (SOV) and in vitro (OPU/IVEP) embryo production. (scielo.br)
Complexes2
  • In the first part of the study, cumulus-oocytes complexes (COCs) (n=434) were aspirated from 60 ovaries. (macvetrev.mk)
  • The cumulus-oocyte-complexes (COCs) recovery rates with respect to reproductive status per sei ( Balaenoptera borealis ) and Bryde's ( B. edeni ) whales were determined in Experiment 1. (cambridge.org)
Vivo3
  • The aim of in vitro maturation oocyte systems is to produce oocytes of comparable quality to those derived in vivo. (embrapa.br)
  • 9. Alterations in the cell cycle of mouse cumulus granulosa cells during expansion and mucification in vivo and in vitro. (nih.gov)
  • Extracorporeal Shock Waves (ESW), widely used for the treatment of urolithiasis, have been reported to cause modifications of cell growth both in vitro and in vivo. (biolifesas.org)
Expanded cumulus cells1
  • The COCs were classified into either Grade A (COCs with five or more layers of compact cumulus cells) or Grade B (COCs with less than five layers of compact or expanded cumulus cells) before being cultured for IVM. (cambridge.org)
Developmental1
  • These findings showed that COCs with high morphological characteristics had higher developmental ability compared to COCs with low morphology. (macvetrev.mk)
Nuclear6
  • The present study was designed to examine the surface morphological changes of the cumulus-oocyte complex (COC) and nuclear maturation in a culture system containing pyruvate. (embrapa.br)
  • Of the many factors related to nuclear reprogramming of donor nuclei, it was reported that the maturation promoting factor (MPF) activity is important [ 23 ]. (koreamed.org)
  • The selection of oocytes with high quality is not important only for in vitro production (IVP), but also for other procedures such as pronuclear microinjections, intracytoplasmic sperm injection and nuclear transplantation ( 4 ). (macvetrev.mk)
  • Incomplete nuclear and ooplasmic maturation of the oocytes has an impact on unsuccessful fertilization. (macvetrev.mk)
  • Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. (animbiosci.org)
  • In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. (animbiosci.org)
Ovaries2
  • The COCs were harvested from the ovaries and in vitro maturation was evaluated in San Marcos (SM) medium, a chemically defined culture system containing 22.3 mM sodium pyruvate. (embrapa.br)
  • Oocytes used for in vitro production are most frequently retrieved from ovaries obtained from slaughterhouses. (macvetrev.mk)
Blastocyst1
  • Recent studies suggest that RO-3306 reversibly inhibits spontaneous resumption from diplotene arrest without affecting their further progression to M-II stage and development to blastocyst stage in porcine oocytes cultured in vitro [25]. (cgrpreceptor.com)
Mammalian1
  • One of the major downstream factors that regulate meiotic cell cycle in oocytes/eggs is maturation promoting factor (MPF) in most of the mammalian species [1-4]. (cgrpreceptor.com)
Morphological3
  • Brilliant cresyl blue (BCB) staining is a method used for selection of developmentally competent porcine oocytes, in addition to the standard morphological classification. (macvetrev.mk)
  • The aim of the current study was to assess the relationship between BCB staining in porcine oocytes with high and low morphological characteristics and its maturation rate. (macvetrev.mk)
  • Therefore, compared with conventional morphological selection methods, BCB staining seems to be a reliable test for selecting mature porcine oocytes. (animbiosci.org)
Meiotic2
  • These substances have been shown to have a negative effect on human health and reproduction even in very low doses, and they are endocrine disruptors affecting, among other things, the process of meiotic maturation. (nusl.cz)
  • Cdk1 activity increases during meiotic resumption from diplotene arrest in oocytes cultured in vitro [18-22]. (cgrpreceptor.com)
Inhibits1
  • supported by the observations that MPF kinase inhibitor such as roscovitine inhibits spontaneous resumption of meiosis in porcine as well as rat oocytes cultured in vitro [14,15]. (cgrpreceptor.com)
Resumption1
  • It is well documented that CCs surrounding the oocyte are necessary for resumption of meiosisand full maturation of the gamete. (biolifesas.org)
Concentrations1
  • Methods: Freshly ovulated M-II arrested eggs were exposed to various concentrations of RO-3306 for 3 h in vitro. (cgrpreceptor.com)
Characteristics2
  • The BCB-staining in high-quality oocytes did not have a significant impact on their maturation ability compared to a control group, but it might be useful for selecting developmentally competent oocytes with low morphology characteristics. (macvetrev.mk)
  • This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. (animbiosci.org)
Protein1
  • The present study was conducted to examine effects of cysteamine in culture medium on progression of meiosis, glutathione (GSH) content, kinase activities (histone H1 kinase and mitogen-activated protein kinase), and male pronuclear formation after in vitro insemination of cumulus-denuded oocytes (DOs) in the pig. (elsevierpure.com)
Activity2
  • 8 ) suggested a simple method for selection of porcine oocytes based on the enzymatic activity of glucose-6-phosphate dehydrogenase (G6PDH), a component of the pentose cycle synthesized during the oogenesis. (macvetrev.mk)
  • Postovulatory aging increased Thr14/Tyr15 phosphorylated Cdk1 level, decreased Thr161 phosphorylated Cdk1 as well as cyclin B1 levels and increased Cdk1 activity in aged eggs cultured in vitro. (cgrpreceptor.com)
Produce1
  • Porcine SCNT has been performed to produce transgenic pigs for use in xenotransplantation, and many transgenic pigs have been produced over the past decade [ 9 10 11 ]. (koreamed.org)
Role1
  • These results indicate that pig oocytes can be successfully matured in a chemically definedmedium and suggest a possible bifunctional role of pyruvate as an energy substrate and as an antioxidant protecting oocytes against the stress of the in vitro environment. (embrapa.br)
Control1
Reliable1
  • Currently, there is no reliable method for measurement of ooplasmic maturation ( 3 ). (macvetrev.mk)