Vitro maturation cumulus cells. Medical search. Frequent questions

In vitro maturation (IVM) is the technique of letting the contents of ovarian follicles and the oocytes inside mature in vitro. (wikipedia.org)
By 1991, the first human pregnancy was recorded using IVM followed by IVF, and in 1994 the first birth using IVM oocytes from polycystic ovarian syndrome patients was recorded highlighting that PCOS patient's oocytes are capable of maturation. (wikipedia.org)
This leads to improved maturation and quality of the oocytes. (wikipedia.org)
Oocytes are classified depending on their condition, such as number of cumulus cells. (wikipedia.org)
The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. (wikipedia.org)
After recovering the Cumulus oocytes complex, where matured in vitro for 24 hours in static a condition that include for juvenile oocyte, the supplementation with hundred micromolar of trehalose in maturation medium. (jove.com)
Following in vitro maturation, the oocytes were denuded of Cumulus cells mechanically by gently pipetting and examined under stereo microscope, with 60x magnification. (jove.com)
This study was conducted to evaluate in vivo and in vitro development of in vitro-matured equine oocytes fertilized by intracytoplasmic sperm injection. (bioone.org)
Oocytes were collected from slaughterhouse-derived ovaries, matured in vitro, and injected with frozen-thawed stallion sperm. (bioone.org)
Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)
For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
Poor ovarian responders (POR) are women undergoing in-vitro fertilization who respond poorly to ovarian stimulation, resulting in the retrieval of lower number of oocytes, and subsequently lower pregnancy rates. (biomedcentral.com)
The follicular fluid (FF) provides a crucial microenvironment for the proper development of follicles and oocytes through tightly controlled metabolism and cell signaling. (biomedcentral.com)
Growth of the dominant follicle encompasses enlargement of the oocyte, replication of follicular cells, and formation and expansion of a fluid-filled follicular antrum or cavity, providing a specialized microenvironment for the development of oocytes. (biomedcentral.com)
It has been suggested that immature oocytes may be more resistant to cryodamage due to lower cell volume and lack of metaphase spindle. (juniperpublishers.com)
Even though high rates of nuclear maturation have been reported with cryopreserved immature oocytes instead [ 1 , 3 ], the developmental capacity has been generally low [ 1 ]. (juniperpublishers.com)
Oocytes within the ovarian follicle are surrounded by mural and cumulus granulosa cells, which perform both endocrine and developmental functions, respectively. (juniperpublishers.com)
A critical difference between cryopreserving MII or GV stage oocytes centers on the importance of the intercellular contacts between germinal and somatic cells. (juniperpublishers.com)
MII-stage oocytes do not rely on support from surrounding somatic cells, while cryopreservation of GV-stage oocytes requires maintenance of their communication with viable somatic cells to preserve a mutual interaction [ 4 ]. (juniperpublishers.com)
Characterization of seminal extracellular vesicles subsets used for supplementing in vitro maturation medium of porcine oocytes. (unibo.it)
Gene expression data of cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes. (unibo.it)
Steroid hormones levels produced by cumulus cells in response to seminal extracellular vesicles subsets during in vitro maturation of porcine oocytes. (unibo.it)
Deep sequencing was performed on small RNA from cumulus cells, oocytes at GV and MII stages, as well as in vitro fertilized derived embryos at 60 h post fertilization (4- to 8-cell) and on Day 6 blastocysts. (appliedbioinfo.com)
GH receptor is expressed in human oocytes and cumulus cells, [ 12 , 13 ] and GH has been shown to promote in vitro nuclear maturation of denuded human oocytes. (medscape.com)
[ 14 ] In addition to its direct effect on the oocytes and/or cumulus cells, GH may also influence oocyte quality indirectly, through activation of insulin-like growth factor-I synthesis or promotion of follicle-stimulating hormone-induced ovarian steroidogenesis (reviewed in [ 15 ] ). (medscape.com)
The present study was conducted to determine the effects of cumulus cells and sodium pyruvate during in vitro maturation of bovine oocytes on maturation, fertilization, and subsequent development. (elsevierpure.com)
Cumulus-enclosed oocytes (CEOs) and cumulus-denuded oocytes (CDOs) were cultured for 24 h in polyvinylpyrrolidone-Hepes-tissue culture medium 199 with or without sodium pyruvate. (elsevierpure.com)
Oocytes were fertilized in vitro and then cultured in CR1aa for 10 days. (elsevierpure.com)
Before in vitro fertilization, the glutathione (GSH) content of some oocytes was measured. (elsevierpure.com)
The GSH contents of oocytes significantly decreased in CDOs after maturation culture, but the GSH contents of oocytes in CEOs remained at the same level as oocytes before culture. (elsevierpure.com)
After that, oocytes are collected by slicing and only oocytes with a cytoplasmic are homongent and have compact cumulus cells used in the study. (ifory.id)
in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
Sibling oocytes were randomly allocated after insemination to early cumulus cell removal at 6 hours (group I) and late cumulus cell removal at 16-18 hours (group II). (ecerm.org)
Cumulus cells play a significant role in supporting oocytes and fertilization. (ecerm.org)
Topics this month include: DHEA use for IVF patients (2:07), the ideal number of oocytes to reach live birth (10:47), in vitro maturation with cumulus cells (20:57), and sperm DNA fragmentation (29:12). (libsyn.com)
The effect of resveratrol, melatonin and their combination on alleviating HS was compared according to the maturation rate of oocytes and the development competence of embryos after parthenogenetic activation (PA). (sagepub.com)
Results: Supplementation with resveratrol (2.0 μmol/L) not only improved the nuclear maturation but also raised the blastocyst rate of porcine embryos' PA from oocytes that underwent HS by increasing their glutathione (GSH) level, reducing reactive oxygen species (ROS) and up-regulating the expression of Sirtuin 1 (SIRT1). (sagepub.com)
The disruption of the levels of steroid hormones Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and caused the mature failure of in vitro cultured oocytes. (sagepub.com)
Some reports show that it is possible to isolate immature oocytes from human ovarian tissue retrieved by a cortex biopsy or ovariectomy of non-stimulated ovaries and mature them in vitro. (infertilityschool.ru)
The mature oocytes can be vitrified and stored for in vitro fertilization, which, along with ovarian tissue cryopreservation, is mostly practiced in young cancer patients to preserve their fertility. (infertilityschool.ru)
Four immature, germinal vesicle (GV) oocytes were found and removed from tissue, denuded mechanically by a pipette, and matured in vitro in a maturation medium with added FSH and hCG as well as in co-culture with cumulus cells, which were retrieved by their denudation. (infertilityschool.ru)
Three oocytes matured in vitro to the metaphase II (MII) stage and were vitrified for in vitro fertilization along with ovarian tissue cryopreservation. (infertilityschool.ru)
The devastating impact on fertility in such disorder can be successfully avoided by in vitro maturation of oocytes from excised ovarian tissue. (infertilityschool.ru)
Oocytes are surrounded by cells, called cumulus cells, which regulate and assist the process of egg maturation. (health.am)
The identification of these genes in cumulus cells can serve as a novel, non-invasive marker to identify abnormal oocytes and thus ultimately improve IVF success rates," said Patrizio, professor in the Department of Obstetrics, Gynecology & Reproductive Sciences at Yale. (health.am)
Aceto-orcein staining was conducted to evaluate the maturation of oocytes based on the achievement of metaphase II phase that is indicated by the presence of metaphase plate and/or first polar body. (sciencegate.app)
The aim of this study was to determine the in vitro maturation rate of Bligon goat oocytes supplemented with follicle-stimulating hormone (FSH), and their ability for further embryonic development after in vitro fertilization. (sciencegate.app)
First, matured oocytes were evaluated its morphology based on the expansion of cumulus cells and PB1 extrusion. (sciencegate.app)
Next, 600 oocytes were then stained with 1% aceto-orcein to examine maturation based on changes in the configuration of chromosomes and nuclear membrane breakdown. (sciencegate.app)
To prove the ability of mature oocytes to develop into embryos, 900 oocytes were processed for fertilization in vitro. (sciencegate.app)
Conclusion: FSH supplementation significantly improves oocyte maturation and yields mature oocytes for future embryo development in vitro. (sciencegate.app)
This study aimed to determine the influence of antioxidants on the achievement of In vitro culture (IVC) and sperm's ability to adhere to and penetrate further into In vitro maturated oocytes. (vetmedmosul.com)
To fertilize the oocytes, sperm must undergo a series of physiological and biochemical alterations in vitro (3). (vetmedmosul.com)
Oocytes and embryos are not provided with this level of protection when in vitro production (9). (vetmedmosul.com)
Melatonin supplementation has been shown to promote bovine and porcine oocytes' maturation rate and increase their blastocyst of IVF embryos (16). (vetmedmosul.com)
We applied Affymetrix gene expression profiling in CC of oocytes at different maturation stages using either GnRH agonists or GnRH antagonists. (elsevierpure.com)
Considering that in cattle many of the oocytes aspirated and used for the in vitro production of embryos (PIVE) have not yet acquired the competence for the development one must look for alternatives that can increase this competence and, consequently, the efficiency of the PIVE. (ufla.br)
The aim of this study was to determine the histone acetylation pattern in oocytes of different abilities before, during and after in vitro maturation (IVM), and to evaluate whether the use of scriptaid (HDAC inhibitor) before and after meiosis resumption development competence. (ufla.br)
On the one hand, reactive oxygen species (ROS) are mandatory mediators for essential cellular functions including the function of germ cells (oocytes and spermatozoa) and thereby the fertilization process. (hindawi.com)

2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
After that, we examined the result of cultured conditioned mass media (CM) of IVF embryos on splenic immune system cells and Madin-Darby bovine kidney (MDBK) cells with stably presented ISG15 promoter-reporter constructs. (bioskinrevive.com)
These cells exhibited a particular upsurge in ISG15 mRNA appearance and promoter activity when treated using the CM of IVF embryos, recommending that IVF embryos possess the potential to create and discharge IFNT. (bioskinrevive.com)
Small RNA Profile of the Cumulus Oocyte Complex and Early Embryos in the Pig. (appliedbioinfo.com)
Detectable - though low - PIF levels were secreted already by 2-cell stage mouse embryos. (biomedcentral.com)
Two-cell stage embryos derived from the mating of transgenic rats with wild-type rats can be successfully cryopreserved by conventional two-step freezing in the presence of 10% dimethylsulfoxide. (go.jp)
The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
ABSTRACT We aimed to assess the effects of melatonin in the in vitro production of bovine embryos. (sciencegate.app)
Our experiment was further divided into two: the first was to assess the effect of different concentrations of melatonin (treatments) on the maturation rate of COCs, and the second was to assess the effects of melatonin treatments on the in vitro production of bovine embryos. (sciencegate.app)
Glutamine supplementation enhances development of in vitro -produced porcine embryos and increases leucine consumption from the medium[J]. Biol Reprod,2018,99:938-948. (magtechjournal.com)
Use of pregnancy-associated plasma protein-A during oocyte in vitro maturation increases IGF-1 and affects the transcriptional profile of cumulus cells and embryos from Nelore cows[J]. Mol Reprod Dev,2019,86:1694-1704. (magtechjournal.com)
SECM technique may be a valuable tool for accurately assessing the quality of embryos and thereby contribute to improving outcomes associated with assisted reproduction, including human in vitro fertilization. (bioone.org)
Analysis of global gene expression in bovine embryos produced under different conditions can provide valuable information to optimize culture media for in vitro embryo production. (biomedcentral.com)
Importantly, in vitro production in the presence of serum appeared to have a different impact on the embryos according to their sex, with male embryos having three times more genes differentially expressed than their female counterparts (1283 vs. 456). (biomedcentral.com)
Male embryos appeared to be most affected by suboptimal in vitro culture, i.e. in the presence of serum. (biomedcentral.com)
Preimplantation genetic testing (PGT) is a technique used to identify chromosomal genetic abnormalities in embryos created through in vitro fertilization (IVF) before pregnancy. (medscape.com)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)

It can be offered to women with infertility problems, combined with In Vitro Fertilization (IVF), offering women pregnancy without ovarian stimulation. (wikipedia.org)
In 1935, Pincus & Enzmann did the first experiment on immature rabbit oocyte, showing in vitro spontaneous maturation and fertilization. (wikipedia.org)
fertilization (IVF) for IFNT creation and IFN responsiveness to various other cells. (bioskinrevive.com)
Components and OPTIONS FOR in vivo test: In vitro maturation and fertilization maturation and fertilization had been carried out as described [21] previously. (bioskinrevive.com)
Being able to reproduce those conditions in vitro could lead to successful oocyte maturation and positive fertilization outcomes. (juniperpublishers.com)
the expansion of somatic cells surrounding the oocyte and the maturation of the cytoplasm to support fertilization and early embryonic development [ 5 ]. (juniperpublishers.com)
Background and Objective Administration of growth hormone (GH) during ovarian stimulation has been shown to improve success rates of in vitro fertilization. (medscape.com)
Administration of growth hormone (GH) during ovarian stimulation has been shown to improve success rates of in vitro fertilization (IVF) treatment, [ 1 , 2 ] and especially in women with poor ovarian response. (medscape.com)
Maturation and normal fertilization rates of CDOs cultured with sodium pyruvate and CEOs were higher than that of CDOs cultured without sodium pyruvate. (elsevierpure.com)
This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)
Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
The impact of early mechanical removal of cumulus cells on fertilization and embryonic development is not yet precisely known. (ecerm.org)
This study aimed to investigate the effects of early and late cumulus cell removal on fertilization, polyspermy, embryonic development potential, blastocyst development, and clinical outcomes. (ecerm.org)
A prospective study was conducted of patients who underwent in vitro fertilization between September 2019 and October 2020. (ecerm.org)
If total fertilization failure (TFF) was determined to have occurred at early cumulus cell removal, rescue intracytoplasmic sperm injection (ICSI) was performed. (ecerm.org)
Early cumulus cell removal at 6 hours had no significant difference in fertilization, polyspermy, embryo development, or obstetric and perinatal outcomes compared to late removal. (ecerm.org)
Removal of the cumulus cells at this moment can markedly decrease fertilization. (ecerm.org)
In conventional in vitro fertilization (IVF), co-incubation of the cumulus-oocyte complex and sperm for 16-18 hours can imitate natural conditions, but prolonged exposure time may generate high levels of reactive oxygen species produced by cumulus cells and excess spermatozoa, resulting in impaired embryo development and vitality [ 1 , 2 ]. (ecerm.org)
However, an additional benefit of early cumulus cell removal is early recognition of fertilization based on the presence of a second polar body, and rescue ICSI can be conducted in cases of failed or low fertilization with promising outcomes [ 7 - 9 ]. (ecerm.org)
This discovery may soon allow them to avoid using abnormal - or aneuploid - eggs during infertility treatments, and instead to pick eggs that are healthy enough for a successful in vitro fertilization (IVF) cycle. (health.am)
Assisted reproductive technologies (ARTs) are techniques to help a woman become pregnant, including in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), and other similar procedures. (health.am)
More than 4 million babies have been born worldwide as a result of using the in vitro fertilization technique. (health.am)
In the last decade, in vitro fertilization and sperm capacitation techniques have significantly advanced. (vetmedmosul.com)
ROS are free radicals that are continuously produced by oxidative metabolic reactions and increase in vitro conditions, causing aging of both the oocyte (7) and sperm (8), as well as perhaps impairing fertilization and embryo development. (vetmedmosul.com)
Non-invasive amino acid profiling of embryo culture medium using HPLC correlates with embryo implantation potential in women undergoing in vitro fertilization[J]. Front Physiol,2020,doi:10.3389/fphys.2020.00405. (magtechjournal.com)
Association of soluble HLA-G plasma level and HLA-G genetic polymor-phism with pregnancy outcome of patients undergoing in vitro fertilization embryo transfer[J]. Front Immunol,2020,doi:10.3389/fimmu.2019.02982. (magtechjournal.com)
Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
In in vitro fertilization (IVF) cycles controlled ovarian hyperstimulation (COH) is established by gonadotropins in combination with gonadotropin-releasing hormone (GnRH) agonists or antagonists, to prevent premature luteinizing hormone (LH) surge. (elsevierpure.com)
However, the exposure of these cells to excessive levels of oxidative stress by too high levels of ROS or too low levels of antioxidative protection will render these cells dysfunctional thereby failing the fertilization process and causing couples to be infertile. (hindawi.com)
However, these techniques have to be carried out very carefully as they, if applied wrongly, bear risks of generating ROS damaging the germ cells and preventing fertilization. (hindawi.com)
This has been shown for intrauterine insemination (IUI), in vitro fertilization (IVF), and ICSI, and increased incidences of chromosomal abnormalities, minor and major birth defects, or early childhood cancers, particularly in the male offspring, have been linked to these procedures [ 8 - 16 ]. (hindawi.com)
Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
The use of preimplantation genetic testing for aneuploidy (PGT-A), formerly known as preimplantation genetic screening or PGS, has increased in recent years, now encompassing an estimated 40% of in vitro fertilization (IVF) cycles in the United States. (medscape.com)
in vitro fertilization. (who.int)

Whether the maturation happens in vivo or in vitro, the environment to which the oocyte and its surrounding cells are exposed, affects the developmental competence of the oocyte and subsequent embryonic development [ 5 ]. (juniperpublishers.com)
Small RNA represent several unique non-coding RNA classes that have important function in the development of germ cells and early embryonic development. (appliedbioinfo.com)
Rho GTPases and their downstream effector proteins regulate a diverse array of cellular processes during embryonic development, including reorganization of cytoskeletal architecture, cell adhesion, and transcr. (biomedcentral.com)
Nuclear maturation, gene expression, cumulus cell expansion, and embryonic development and quality were analyzed. (ufla.br)

Cytotoxic And Endocrine-Disrupting Potential Of atrazine, diazinon, endosulfan and mancozeb in adrenocortical steroidogenic cells of rainbow trout in vivo. (innspub.net)
However, DFMO had been mainly without effect of these inside vitro or even in vivo assays. (hsp90-inhibitors.com)

Through in vitro maturation, folliculogenesis and latter parts of oogenesis are being mimicked outside of the ovaries- trying to recreate the conditions for these processes. (wikipedia.org)
During the course of folliculogenesis, growth is achieved by cell proliferation and formation of follicular fluid, whereas development involves cytodifferentiation of all the cells and tissues in the follicle. (glowm.com)
Specialized hypothalamic neurons secrete pulses of gonadotropin-releasing hormone (GnRH) into the portal blood vessels, which acts on the gonadotrophs to cause a pulsatile release of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which act on ovarian follicle cells to control folliculogenesis. (glowm.com)
This study showed ginseng protects folliculogenesis via alteration of hypothalamic- pituitary-gonadal (HPG) axis, induction of proliferation in ovarian somatic cells, reduction of lipid peroxidation, and downregulation of apoptotic markers in the mouse ovary, treated with nicotine. (biomedcentral.com)

The aim of our study was to improve the understanding of gene expression profile of cumulus cells (CC) in terms of ovarian stimulation protocol and oocyte maturity. (elsevierpure.com)

RESULTS: In vitro, SFN downregulated the mRNA expression of inflammatory factors (tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß and IL-6), inhibited the protein expression of inflammatory mediators (cyclooxygenase-2 (COX2), and inducible nitric oxide synthase (iNOS)) while suppressing nuclear factor kappa-B (NF-κB) activation in LPS-induced GMECs. (bvsalud.org)
These results indicate that sodium pyruvate promotes nuclear maturation of bovine CDOs and that a continuing presence of cumulus cells during maturation is important for subsequent development of zygotes to the blastocyst stage. (elsevierpure.com)
Non-invasive nuclear magnetic resonance analysis of male and female embryo metabolites during in vitro embryo culture[J]. Metabolomics,2018,25:113. (magtechjournal.com)
Inhibition of nuclear meiotic maturation via IBMX significantly increased MIR21 and decreased its target, PDCD4. (biomedcentral.com)

Similar to the expression analysis of other species, these data demonstrate dynamic expression regulation of multiple classes of non-coding RNA during oocyte maturation and development to the blastocyst stage. (appliedbioinfo.com)
The 40 min cultured group had a higher rate of blastocyst formation and number of total cells in blastocysts than the 20 min cultured group. (e-jarb.org)
[ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)

Primordial follicles containing the primary oocyte, arrested at prophase of meiosis I, develop into primary follicle containing cuboidal granulosa cells. (wikipedia.org)
A secondary follicle is formed with a few granulosa cell layers, as well as a theca layer. (wikipedia.org)
Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
Increases in the amount of PAPP-A mRNA in granulosa cells during follicular development occurs in some but not all species, indicating that other proteases or protease inhibitors may be involved in IGFBP degradation. (bioone.org)
A key step for this is to understands the interactions between somatic granulosa cells and the oocyte that are required for maturation and developmental competence. (juniperpublishers.com)
We focus primarily on the role of both types of granulosa cells: mural granulosa cells, which perform endocrine functions, including hormone production and cumulus cells, which provide metabolic support and establish bi-directional communication with the oocyte through various pathways, including direct contact. (juniperpublishers.com)
The coordinate development of both granulosa cell types is necessary to fulfill oocyte requirements. (juniperpublishers.com)
In both cases, being able to reproduce the conditions in vitro by recreating the follicle microenvironment using cultured granulosa cells may be necessary for successful oocyte maturation, either before or after freezing. (juniperpublishers.com)
[7] The cells that comprise the follicle, known as granulosa cells, are connected to each other by proteins known as gap junctions, that allow small molecules to pass between the cells. (wikipedia.org)
The granulosa cells produce a small molecule, cyclic GMP , that diffuses into the oocyte through the gap junctions. (wikipedia.org)
Luteinizing hormone acts on receptors in the outer layers of granulosa cells of the follicle, causing a decrease in cyclic GMP in the granulosa cells. (wikipedia.org)
[5] Because the granulosa cells and oocyte are connected by gap junctions, cyclic GMP also decreases in the oocyte, causing meiosis to resume. (wikipedia.org)
re- that heat stress strongly inhibited gonadotropin receptor ported that HS caused relatively low plasma concentration levels and aromatase activities in granulosa cells. (sagepub.com)
The remaining cells in the follicle wall ( i.e. granulosa, membrana and periantral, theca, the theca interna and externa) develop into the corpus luteum. (glowm.com)
Nicotine induced a reduction in the proliferative indexes of granulosa and theca cells in pre-antral and antral follicles ( P = 0.001). (biomedcentral.com)
Co-Treatment with ginseng also reduced the expression of apoptotic markers and increased the proliferative indexes in granulosa and theca cells in pre-antral and antral follicles and also in stroma cells, in comparison to the nicotine group ( P = 0.001). (biomedcentral.com)
HN - 2008 BX - Von Ebner's Glands MH - Cumulus Cells UI - D054885 MN - A05.360.319.114.630.535.200.500 MN - A06.407.312.497.535.300.500 MN - A11.436.300.500 MS - The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. (bvsalud.org)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

This study was aimed to determine the effect of urea in maturation medium on in vitro oocyte maturation rate. (sciencegate.app)
Results: The results indicated that FSH supplementation significantly increased oocyte maturation rate (65.21±7.26 vs. 43.25±6.23%) as indicated by extrusion of PB1 and homologous chromosome pairing and lined in the equator. (sciencegate.app)
Injection of a MIR21 mimic increased oocyte maturation rate. (biomedcentral.com)

Our results show that the direct targeting of HAS2 by miR-574 negatively impacts oocyte quality during IVM and that inhibiting miR-574 derepresses HAS2 expression and subsequently improves oocyte maturation. (appliedbioinfo.com)

biopsied human ovarian tissue and achieved a 10% rate of maturation from unilaminar follicles into metaphase II by a multi-step culture system: 8 days of culture in a serum-free medium 8 days of culture in a serum-free medium with activin A 4 days of culture on membranes with activin A and follicle-stimulating hormone (FSH). (wikipedia.org)
A promising avenue of research is to replicate the microenvironment of the follicles to achieve careful cryopreservation and successful in vitro maturation. (juniperpublishers.com)
In article 1, cumulus-oocyte complex (COCs) were obtained from follicles 1-3mm, considered less competent (IC) and 6-8mm, more competent (CP). (ufla.br)
Only a few follicles in the human ovary survive to complete the cytodifferentiation process, with 99.9% dying by a programmed cell death mechanism called apoptosis. (glowm.com)

Initially the arrest is due to lack of sufficient cell cycle proteins to allow meiotic progression. (wikipedia.org)
Maintenance of meiotic arrest also depends on the presence of a multilayered complex of cells, known as a follicle, that surrounds the oocyte. (wikipedia.org)
Germinal vesicle breakdown (GVBD) occurs during oocyte meiotic maturation, a period when transcriptional processes are virtually inactive. (biomedcentral.com)
Our results indicate MIR21 is active and important during meiotic maturation of the oocyte. (biomedcentral.com)
We report that CK2 is essential for porcine oocyte meiotic maturation by regulating spindle assembly checkpoint (SAC). (biomedcentral.com)
Immunostaining and immunoblotting analysis showed that CK2 was constantly expressed and located on the chromosomes during the entire oocyte meiotic maturation. (biomedcentral.com)
In the current study, we explored the functional roles of CK2 on the porcine oocyte meiotic maturation. (biomedcentral.com)
The further research is needed to better understand the new important mechanisms that regulate diffcult aspects of oocyte meiotic maturation in mammals. (kiev.ua)

These microinsemination techniques can be combined with cryopreservation of sperm and spermatid cells, leading to successful transmission of transgenes carried in male gametes. (go.jp)
Cumulus cells enhance capacitation, sperm binding and penetration of the zona pellucida, passage through the perivitelline space, binding and fusion to the oolemma, activation of the cortical reaction, and formation of the male pronucleus. (ecerm.org)
In current practice, short co-incubation of the cumulus-oocyte complex with sperm and early cumulus cell removal (6 hours) is challenging [ 4 - 7 ]. (ecerm.org)
For this purpose, we have incubated ram sperm using four different treatments in terms of antioxidants: melatonin, cysteamine, vitamin C, and vitamin E. They were incubated by the standard methods of maturation and capacitation of sperm. (vetmedmosul.com)
Oxidative stress is one of the key factors contributing to a decrease in sperm quality during the maturation and capacitation of the sperm (5). (vetmedmosul.com)

Photomicrograph of ovulation shows the expanded egg-cumulus complex leaving the follicle through the stigma. (glowm.com)

Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
Metabolic control of oocyte development: linking maternal nutrition and reproductive outcomes[J]. Cell Mol Life Sci,2015,72:251-271. (magtechjournal.com)
In the farming setting, temperature outcomes in which probably interrupt food accessibility might be more essential as compared to outcomes IKK-16 in vitro immediately affecting your success of Whitened Stork children. (hsp90-inhibitors.com)

Cumulus oocyte complexes (COCs) of cows derived from follicle aspiration were divided into three groups. (sciencegate.app)
In control group (P0), the COCs were matured in vitro in a maturation medium without urea addition, meanwhile in the P1 and P2 groups, the medium was added with urea 20 and 40 mg/dL, respectively. (sciencegate.app)
The cumulus-oocyte complexes (COCs) were distributed among treatments at concentrations of 0, 10-1, 10-3 and 10-5 µMol/L melatonin. (sciencegate.app)
In article 2 COCs were submitted to pre-maturation (PIVM) for 6 hours in the presence or absence of scriptaid (HDAC inhibitor). (ufla.br)

In the present work we present a succinct review of these molecular interactions, including endocrine and paracrine signaling, gene regulation and cell communication. (juniperpublishers.com)
HS had nega- * Correspondence: [email protected] tive effects on oocyte maturation via down-regulation of Equal contributors the gene expression in steroid hormone synthesis or their National Engineering Laboratory for Animal Breeding, Key Laboratory of receptors. (sagepub.com)
Microarray-based global gene expression analyses on bovine placenta and trophoblast cells suggest that the expression profiles of specific genes depend on the cells and tissues in which they are expressed as well as the time of the gestation period. (bioone.org)
We used RNA sequencing to examine the effect of in vitro embryo production, in either serum-containing or serum-free media, on the global gene expression pattern of individual bovine blastocysts. (biomedcentral.com)

In this video, we describe a protocol for the vitrification of sheep oocyte, collected from both juvenile and adult donors, and in vitro matured to prior to cryopreservation. (jove.com)

The pathways affected by in vitro production differed depending on the type of supplementation. (biomedcentral.com)

Diagram showing the reduction in number of the chromosomes in the process of maturation of the ovum . (wikipedia.org)
They characterized two genes - SPSB2 and TP5313 - and found that the expression of these genes was consistently underrepresented in cumulus cells that surrounded abnormal eggs, while these same genes were normally expressed in eggs with the correct number of chromosomes. (health.am)

It was also found −7 −7 that melatonin (10 mol/L) and the combination of resveratrol (2.0 μmol/L) plus melatonin (10 mol/L) exhibited more potent effects than resveratrol alone regarding their protective activities on oocyte maturation under HS. (sagepub.com)
Keywords: Combination, Maturation Melatonin, Oocyte, Porcine, Resveratrol Background secretion by luteal cells and increased the follicle-stimu- Although modern cooling systems are used for preserving lating hormone (FSH) [4]. (sagepub.com)

Here, we found that miR-574 directly targets the transcript for hyaluron synthase 2 protein (HAS2), a key enzyme in the production of extracellular matrix by the surrounding cumulus cells. (appliedbioinfo.com)
Integrins are heterodimeric transmembrane glycoproteins involved in cell-cell and cell-extracellular matrix adhesion. (go.jp)
Cyclophilin A (CypA), which has peptidyl-prolyl cis-trans isomerase (PPIase) activity, regulates multiple functions of cells by binding to its extracellular receptor CD147. (bvsalud.org)

This results in an expanding or dispersed pattern of the cumulus oophorus around the egg cell, facilitating its identification within follicular fluid. (wikipedia.org)

[1] is the differentiation of the ovum (egg cell) into a cell competent to further develop when fertilized. (wikipedia.org)
oocytogenesis , ootidogenesis , and finally maturation to form an ovum (oogenesis proper). (wikipedia.org)

Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)

One thousand five hundred cumulus-oocyte complexes were matured for 24 h in tissue culture medium 199 supplemented with 50 IU/L FSH or without FSH (control). (sciencegate.app)

His research interests focus on in vitro fertilisation and assisted reproduction, impacts of reproductive ageing and disorders such as endometriosis, premature ovarian failure and polycystic ovary syndrome on fertility and quality of life, reproductive effects of cancer treatment and onco-fertility and health economic and demographic aspects of infertility. (acemws2023.com)

In this study, Yale Fertility Center director Pasquale Patrizio, M.D., and Dagan Wells of the University of Oxford studied genes expressed in the cumulus cells. (health.am)
They were able to identify a set of genes that are less active in cells that are associated with abnormal eggs. (health.am)
This custom-made microarray revealed that trophoblast-specific genes such as placental lactogen, pregnancy-associated glycoproteins, prolactin-related proteins, and those of the sulfotransferase family were mainly expressed in the trophoblast giant cells and that their expression increased as gestation progressed. (bioone.org)
Among other differentially expressed genes we observed a marked number of new genes connected to cell adhesion and neurotransmitters such as dopamine, glycine and γ-Aminobutyric acid (GABA). (elsevierpure.com)

Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)

The in vitro embryo production (IVEP) is very stressful for gametes. (vetmedmosul.com)
As a result, developing a culture medium capable of effectively reducing ROS in vitro embryo production (IVEP) protocols is of tremendous importance (10). (vetmedmosul.com)

Oogenesis takes place during fetal life, in which primordial germ cells undergo mitosis until a few weeks prior to birth, forming oogonia. (wikipedia.org)

The role of glucose metabolism on porcine oocyte cytoplasmic maturation and its possible mechanisms[J]. PLoS One,2016,11:e0168329. (magtechjournal.com)

Oocyte meiosis, important to all animal life cycles yet unlike all other instances of animal cell division, occurs completely without the aid of spindle -coordinating centrosomes . (wikipedia.org)

Early cumulus cell removal combined with early rescue ICSI may have the potential to help couples with TFF. (ecerm.org)

Our findings demonstrate that CK2 promotes the porcine oocyte maturation by ensuring normal spindle assembly and DNA damage repair. (biomedcentral.com)

Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)
Since the development of in vitro embryo production in cattle, different supplements have been added to culture media to support embryo development, with serum being the most popular. (biomedcentral.com)

Further knowledge of the integrin-mediated regulation of cell growth and cell survival may facilitate our understanding of many key aspects of development and physiology in reproduction. (go.jp)
Scientists attempting to discover the basic requirements for successful embryo development have a hurdle when using in vitro embryo manufacturing strategies (2). (vetmedmosul.com)
Poor in vitro embryo development is a major obstacle in porcine assisted reproduction. (mdpi.com)
Since the initial development of in vitro embryo production, the technique has been applied successfully to many species for clinical, commercial, and research purposes. (biomedcentral.com)

Exogenous testosterone decreases intratesticular testosterone production, thus inhibiting Sertoli cell function and spermatogenesis. (medscape.com)

A previous screen of small RNA in the porcine ovary identified the down regulation of miR-574 during oocyte maturation, although its role during this process was not established. (appliedbioinfo.com)
Moreover, his work on the hormonal regulation of gap junction formation and growth culminated in an authoritative review article in Tissue and Cell, "Structural Diversity of Gap Junctions (1988)", which became a citation classic. (clinanat.com)
CK2 (casein kinase 2) is a serine/threonine-selective protein kinase that has been involved in a variety of cellular processes such as DNA repair, cell cycle control and circadian rhythm regulation. (biomedcentral.com)

2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)

Adipose stem cells in reparative goat mastitis mammary gland. (sciendo.com)
Goat mammary epithelial cells (MECs) were isolated and treated with 2.5, 5, 10 and 20 µg/mL lipopolysaccharide (LPS) respectively for a different time, S100A7 mRNA expression and protein secretion were upregulated significantly with LPS treatment for 3 h, and the secretion level of S100A7 descended after 48 h treatment for all of these four groups. (bvsalud.org)
During the culture of goat mammary epithelial cells (GMECs), high RANKL level additions promote the Inhibitor kappaB (IκB)/p65/Cyclin D1 expression related to cell proliferation and decrease the phosphorylated signal transduction and transcription activator 5 (Stat5) expression related to milk-protein synthesis of GMECs, which is consistent with electron microscope results showing that there are fewer lactoprotein particles in the acinar cavity of a firm mammary. (bvsalud.org)
This study aimed to explore the anti-oxidant and anti-inflammatory effects and potential molecular mechanisms of SFN in lipopolysaccharide (LPS)-induced primary goat mammary epithelial cells (GMECs) and a mouse model of mastitis. (bvsalud.org)
In vitro maturation allows female goat (does) contributes toward reproduction despite the fact that the animal has been slaughtered. (sciencegate.app)

Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)

Growth hormone (GH) is a peptide hormone secreted by the anterior pituitary gland in pulsatile manner, and it has important roles in cell growth and metabolism throughout the body. (medscape.com)
Human chorionic gonadotropin (hCG) is a luteinizing hormone (LH) analogue that may be used alone or in combination with human menopausal gonadotropin (hMG) for Leydig cell stimulation. (medscape.com)

Anatomy20

Organisms10

Diseases2

Chemicals and Drugs38

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes36

Information Science1

Health Care1

Oocytes59

Embryos23

Fertilization35

Embryonic4

Vivo2

Folliculogenesis4

Profile of cumulus cells1

Nuclear4

Blastocyst3

Granulosa18

Oocyte maturation rate3

Improves oocyte maturation1

Follicles4

Meiotic8

Sperm5

Ovulation1

Outcomes3

COCs4

Gene4

Cryopreservation1

Supplementation1

Chromosomes2

Melatonin2

Extracellular3

Oophorus1

Ovum2

Embryo cleavage2

Complexes1

Fertilisation1

Genes4

Complex1

IVEP2

Undergo1

Cytoplasmic1

Meiosis1

ICSI1

Spindle1

Cattle2

Development4

Production1

Regulation3

Induces1

Goat5

Mastitis1

Hormone2