Vitro immune sera. Medical search. Frequent questions

Here, we intend to characterize how and what antibodies are neutralizing SPZ in the tissues and correlate their in vitro activities with protection in vivo. (anr.fr)
To determine the in vivo relevance of this phenomenon, we sought to detect cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons. (jci.org)
The presence of cytotoxin-neutralizing antibodies in sera from H. pylori-infected persons indicates that the cytotoxin is synthesized in vivo. (jci.org)
Knockdown of CASP5 greatly inhibited GBM proliferation and resulted in G1 cell cycle arrest along with higher apoptosis ratios in vitro and in vivo, while overexpression led to the opposite phenomenon. (cancerindex.org)
Through this integration with the FMD-DISCONVAC project, we will be fulfilling the objectives of the EU call KBBE-2008-1-3-02: To substitute vaccine potency tests in animals by assays enabling a correlation of in-vitro tests based on immunological principles of antibody-based effector immune responses operating in vivo. (forschung3r.ch)
Efficacy was correlated with sustained concentration of virus neutralizing mAbs in-vivo rather than their in-vitro neutralizing capacity. (researcher-app.com)
The peptide fraction was then found bioactive, producing endothelial cell inflammation and vascular dysfunction ex vivo akin to that induced with whole serum. (cdc.gov)
To carry out these legislative mandates, ATSDR's Division of Toxicology (DT) has developed and coordinated a mixtures program that includes trend analysis to identify the mixtures most often found in environmental media, in vivo and in vitro toxicological testing of mixtures, quantitative modeling of joint action, and methodological development for assessment of joint toxicity. (cdc.gov)

As viral vectors comprise the risk of immune and inflammatory responses, human serum albumin (HSA) nanoparticles were found to represent a safer and more convenient alternative. (uni-frankfurt.de)
CCL5 is a unique chemokine with distinct stage and cell-type specificities for regulating inflammation, but how these specificities are achieved and how CCL5 modulates immune responses is not well understood. (nature.com)
Therefore, host CCL5 can obviously work as an anticancer molecule to mount relevant immune responses against some cancer cells. (nature.com)
This review paper will 1) present an overview of human physiological responses to cold exposure, 2) present the human studies examining the effects of cold exposure on immune responses, and 3) summarize recent experiments from our laboratories examining the effects of exercise and fatigue on immune responses during subsequent cold exposure. (researchgate.net)
Type-2 (T2) immune responses in airway epithelial cells (AECs) classifies mild-moderate asthma into a T2-high phenotype. (ersjournals.com)
This vaccination regimen also led to the generation of both Th1 and Th2 cellular immune responses in mice. (frontiersin.org)
Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)
We are evaluating the relationship between protection and the capacity of a serum to promote Fc receptor-mediated phagocytosis of FMDV immune complexes by macrophages and dendritic cells in terms of virus destructionn, and Fc receptor-mediated IFN-alpha and cytokine responses in plasmacytoid dendritic cells (see Figure 1). (forschung3r.ch)
These include an assay measuring enhanced infection of bovine monocyte-derived dendritic cells by immune complexed-FMDV and for the porcine system enhanced IFN-alpha responses by plasmacytoid DC. (forschung3r.ch)
In this report, we focused on innate immune responses elicited by inactivated EV71 and CA16 antigens administered intradermally or intramuscularly. (omicsdi.org)
RESULTS: Data-independent mass spectrometry enabled the unbiased quantitative characterization of 841 significant MWCNT-responses within an enriched peptide fraction, with 567 of these factors demonstrating significant correlation across animal-paired bronchoalveolar lavage and serum biofluids. (cdc.gov)
The vaccine was safe and well tolerated, induced potent serum IgG & IgA responses waning post last vaccination, and low and intermittent mucosal IgG, but did not induce neutralizing activity neither in serum nor vaginal samples. (europa.eu)
The etiology of eosinophilic fasciitis is unknown, but aberrant immune responses may play a role because hypergammaglobulinemia and antinuclear antibodies are associated. (medscape.com)
For this reason, the skin is often exposed to potentially hazardous agents, including chemicals, which may contribute to the onset of a spectrum of adverse health effects ranging from localized damage (e.g., irritant contact dermatitis and corrosion) to induction of immune-mediated responses (e.g., allergic contact dermatitis and pulmonary responses), or systemic toxicity (e.g., neurotoxicity and hepatoxicity). (cdc.gov)
This document provides the scientific rationale and framework for the assignment of multiple hazard-specific skin notations (SK) that clearly distinguish between the systemic effects, direct (localized) effects, and immune-mediated responses caused by skin contact with chemicals. (cdc.gov)
Initial findings are that both vaccines are safe and induce promising immune responses in human volunteers. (who.int)

Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori. (jci.org)
This multi-allergen IgE antibody panel, combined with measurement of IgE in serum, is an appropriate first-order test for allergic disease. (mayocliniclabs.com)
2. Antibody-mediated enhancement of SARS-CoV infection with anti-SARS-CoV Spike immune-serum was observed in vitro. (hku.hk)
4. The antibody-mediated enhancement phenomenon is not a mandatory component of the humoral immune response elicited by SARS vaccines, as pure neutralising antibody only could be obtained. (hku.hk)
Based on immunological evidence obtained from previous work, we are developing in-vitro screening assays based on the relationship between protection and the avidity of specific antibody for binding to the pathogen in question. (forschung3r.ch)
Because absorption of parasiticidal rabbit sera with normal monkey erythrocytes abolishes the parasiticidal effect, it is concluded that the effect is largely due to an antibody to the red cells. (rupress.org)

It is a complete serum-free, xeno-free medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use. (thermofisher.com)
ImmunoCult™-XF T Cell Expansion Medium is a serum-free and xeno-free medium optimized for the in vitro culture and expansion of human T cells isolated from peripheral blood. (stemcell.com)

A for- in passive immunization experiments was prepared by pooling malin-inactivated veterinary vaccine (West Nile Virus Vac- convalescent-phase serum samples of six hamsters that were cine, Killed, Fort Dodge Animal Health, Fort Dodge, IA) was bled 5 weeks after infection with WNV strain NY385-99. (cdc.gov)
The work presented in this thesis initially established the stability of serum sCD23 in vitro and the clinical (infection, renal/hepatic function and drugs) and demographic factors (age and sex) which determined the levels of this protein in serum. (soton.ac.uk)
With additional cytokine data further work on serum sCD23 was reported on the factors responsible for autoimmunity and lymphoma formation in chronic hepatitis C virus infection and the peripheral anergy in sarcoidosis. (soton.ac.uk)
These data suggest that varying exposures to lifestyle and infection-related factors may be associated with specific changes in the innate and adaptive immune system, that all contribute to age advancement. (frontiersin.org)
It is commonly believed that exposure to cold environmental temperatures depresses immune function and increases the risk for infection. (researchgate.net)
In 1917, a 12-year-old with features suggestive of MVEV infection recovered after receiving immune serum. (mja.com.au)
5. Occurrence of immune-mediated enhancement of SARS-CoV infection raises safety concerns regarding the use of SARS-CoV vaccine in humans and enables new ways to investigate SARS pathogenesis (tropism and immune response deregulation). (hku.hk)
Accordingly, the proposed solution is to develop realisable and robust in-vitro alternatives to such challenge infection tests. (forschung3r.ch)
When mAbs were used in immunodeficient RAG -/- hosts to limit an ongoing infection we observed a reduction in viral load both with mAbs having potent neutralizing capacity in-vitro as well as mAbs classified as non-neutralizing. (researcher-app.com)

Inhibition of the agglutination of SpA-bearing Staphylococcus aureus cells by purified anti-SpA antibodies was observed in vitro. (omicsonline.org)
The use of hyper-immune eggs as alternative to the use of antibiotics could be advantageous for the large amount of antibodies produced, low cost, the reduction of antigenic variation and very low toxicity. (omicsonline.org)
To systematically investigate the relationship of opsonising antibodies with protection using a large panel of sera, we also have established a reporter system to measure opsonizing antibodies against FMDV. (forschung3r.ch)
Specific complement-fixing antibodies are produced in the serum of rabbits in response to injections of living or dead Plasmodium knowlesi . (rupress.org)
Experiments on passive protection in monkey malaria with serum from rabbits which have received intraperitoneal injections of living or dead P. knowlesi yield no conclusive evidence that protective antibodies are formed. (rupress.org)

After obtaining data indicating the presence of a neutrophil attractant protein-1 (NAP-1)-IgG complex in normal human serum, we developed sandwich ELISAs that could quantify NAP-1 and NAP-1-IgG in mixtures of the two moieties. (nih.gov)
Depletion of factor B from mixtures of normal human serum (NHS) and plasma from a patient with membranoproliferative nephritis (MPP), either by heating or by immune equivalence absorption, blocks C3 breakdown by C3NeF. (jci.org)

This was based on finding elevated serum levels in the autoimmune diseases associated with hypergammaglobulinaemia and autoantibody formation and low levels in those conditions with clinical and histological features of exaggerated cellular immunity such as Chrohn's and coeliac disease. (soton.ac.uk)
Other studies have shown elevated serum levels of manganese superoxide dismutase and tissue inhibitor of metalloproteinase (TIMP-1). (medscape.com)

The disease caused by ehrlichiae is generally undifferentiated but is often associated with leukopenia, thrombocytopenia, and elevated serum hepatic transaminase levels in tick-exposed patients. (cdc.gov)

Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus: comparison with wild-type virus with restricted capacity for in vitro replication. (microbiologyresearch.org)

CTS™ Immune Cell Serum Replacement (SR) is designed to support expansion of in vitro cultured human immune cells such as T cells when added to a basal cell culture media such as CTS™ OpTmizer™ T cell Expansion SFM or CTS™ AIM-V™ SFM. (thermofisher.com)
The source of serum sCD23 as being of predominantly B cell was also discovered by recording the serum levels in patients with varying numbers of normal and abnormal B cells. (soton.ac.uk)
The present study deals with the development of pGL3-PEI-coated HSA nanoparticles and subsequent in vitro testing in cerebellar granular and HeLa cells. (uni-frankfurt.de)
In addition to its roles in the regulation of inflammatory diseases and the maintenance of local immune cells, CCL5 expressed by cancer cells plays diverse roles in shaping cancer microenvironments toward their own survival. (nature.com)
Other studies reported that some cancer cells could repress their own CCL5 expression to hinder migration of anticancer immune cells toward cancer sites 15 , 16 . (nature.com)
Approximately 50% of Helicobacter pylori isolates produce a cytotoxin in vitro that induces vacuolation of eukaryotic cells. (jci.org)
Therefore, we investigated the effects of patients' sera on endothelia cells (ECs) in vitro. (mdpi.com)
This provides the first evidence for the effect of PBC exposure on redistribution of immune cells. (researchgate.net)
moreover, the exposure of peritoneal macrophages (in vitro) to temperatures of 4, 10, 24, and 37°C for 1 hour suggested a close inverse association between incubation temperature and the number of cells capable of phagocytosis. (researchgate.net)
Furthermore, sera from immune donors were able to induce both NK cell degranulation and lysis of ZIKV NS1 CEM-NK R cells in vitro. (elsevierpure.com)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

Subsequent work was the first to record increased levels of serum sCD23 in many of the systemic and organ specific autoimmune diseases. (soton.ac.uk)
Restriction enzyme digestion at sites within the structural region and subsequent transcription in vitro yielded RNA products which were translated efficiently in rabbit reticulocyte lysates to produce proteins of the predicted sizes. (microbiologyresearch.org)

Many serum biomarkers have been evaluated in melanoma but their clinical significance remains a matter of debate. (hindawi.com)
In this paper, a review of the serum biomarkers for melanoma will be detailed and will be discussed from the point of view of their practical usefulness. (hindawi.com)
New serum biomarkers of melanoma progression and metastatic disease are still awaited in order to provide efficient rationale for followup and treatment choices. (hindawi.com)
They hypothesized that IP6 would significantly inhibit cell growth of cutaneous melanoma in vitro. (naturalnews.com)

Another study has show that the fascial inflammatory infiltrate is predominately composed of CD8 + T lymphocytes, macrophages, and fewer eosinophils, suggesting a possible cytotoxic immune reaction in response to possible infectious or environmental agents. (medscape.com)

Combinations of neutralizing mAbs further augmented the antiviral effect and were found to be as potent in protection as polyvalent serum from immune animals. (researcher-app.com)

To further facilitate our understanding between the SARS-CoV-2 spike protein structure andantibody response, for the development of future vaccines with a broader-spectrum of antibodyresponse.At Immune Tech, it is our mission to engage our full capacity, knowledge base, and enthusiasm in oureffort to help combat the novel coronavirus pandemic. (immune-tech.com)

none were inhibited by the milk proteins bovine serum albumin or lactoglobulins. (shengsci.com)

They were more symptomatic with higher exhaled nitric oxide fraction ( F eNO ) and blood and sputum eosinophils, but not serum IgE or periostin. (ersjournals.com)

Other specific initiatives were conducted such as evaluation of the sublingual route for immunization, investigation of the Principal Immunodominant Domain (PID) of gp41, and direct HIV inhibition with an Ag family which demonstrated in vitro antiviral activities comparable to the antiretroviral drug T20 (Fuzeon). (europa.eu)

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that help you meet your research needs and budget requirements. (thermofisher.com)

The consultation concluded that whole blood therapies and convalescent blood serums should be considered as a matter of priority. (who.int)

In this classification, the only marker which has been incorporated for clinical use is lactate dehydrogenase (LDH) since elevated serum LDH has been shown in multivariate analysis to be an independent and highly significant predictor of survival even after accounting for site and number of metastases. (hindawi.com)
Clinical significance of serum soluble IL-2R levels in patients with adult T cell leukaemia (ATL) and HTLV-1 carriers. (shengsci.com)
The levels of soluble IL-2Ralpha (sIL-2Ralpha) in serum were measured in HTLV-1 carriers and ATL patients in order to evaluate their possible correlation with clinical status. (shengsci.com)

The value of serum sCD23 in determining the overall balance of cellular and humoral immunity (regulated by the Th1/Th2 cytokines) in a given disease was proposed. (soton.ac.uk)

CCR5 receptor antagonism inhibits hepatitis C virus (HCV) replication in vitro. (uc.edu)

An inflammatory monocyte immune phenotypic profile associated with age advancement in HIV-negative individuals, while the monocyte profile in blood donors and people with HIV was more reflective of loss of function. (frontiersin.org)
NF-κB is a transcription factor that plays a pivotal role in the highly specific pattern of gene expression observed for immune, inflammatory and acute phase response genes, including interleukin 1, interleukin 8, tumor necrosis factor and certain cell adhesion molecules. (justia.com)
A database search curated for known matrix protease substrates and predicted signaling motifs enabled identification of 73 MWCNT-responsive peptides, which were significantly associated with an abnormal cardiovascular phenotype, extracellular matrix organization, immune-inflammatory processes, cell receptor signaling, and a MWCNT-altered serum exosome population. (cdc.gov)

The authors are not aware of previous studies of the primary immune system response developed in eggs from laying hens, so this research could set a precedent in the field of egg white immunoglobulin technology. (omicsonline.org)
45 There is also a report of a man with JEV recovering after being administered non-immune intravenous immunoglobulin. (mja.com.au)

Antisense molecules, adenosine and guanosine nucleosides, including ring-expanded 'fat' nucleoside analogues, brassinosteroids, coumarins, peptide inhibitors, modulators of cholesterol synthesis and a variety of natural products have been screened for efficacy and toxicity both in vitro and in animals. (usu.edu)
The cause of the accentuated and/or accelerated aging in people with HIV remains to be fully elucidated, with factors like HIV associated chronic immune activation and dysfunction, ART toxicity and the higher prevalence of traditional risk factors each having been implicated to contribute to age-associated comorbidity development ( 8 , 9 ). (frontiersin.org)

12] However, the findings concerning the effects of cold exposure on human immune function are inconsistent. (researchgate.net)

As a group, sera from 29 H. pylori-infected patients neutralized the activity of the purified cytotoxin to a significantly greater extent than sera from 24 uninfected persons (P = 0.007). (jci.org)
fourteen of 15 patients had hepatomegaly, five had ascites, and six had serum albumin below 3 g dl-1. (liverpool.ac.uk)
Further, serum factor B levels are often low in patients with membranoproliferative nephritis and bear a rough inverse correlation with C3NeF levels. (jci.org)
HBV and HIV/HBV Infected Patients Have Distinct Immune Exhaustion and Apoptotic Serum Biomarker Profiles. (uc.edu)

Induction of binding Abs was measured by ELISA and neutralizing activity by PBMC and TZM-bl assays in all serum and vaginal samples. (europa.eu)

The hamster passive immunization with immune serum for preventing model of WNV encephalitis has been described (5,6). (cdc.gov)
Overseas experience with WNV and JEV encephalitis suggests that IgM in serum usually appears 4-9 days after disease onset and can persist for months. (mja.com.au)

Neutralization of cytotoxin activity by human or immune rabbit sera was associated with immunoblot IgG recognition of an 87-kD H. pylori protein. (jci.org)

We evaluated the isotype specificity of ZIKV-specific Abs in immune sera and supernatants from stimulated immune PBMC and found that Abs against ZIKV NS1 and virus-like particles were predominantly of the IgG1 isotype. (elsevierpure.com)

Results implicate receptor ligand functionality in driving systemic effects, exemplified by an identified 59-mer thrombospondin fragment, replete with CD36 modulatory motifs, that when synthesized produced an anti-angiogenic response in vitro matching that of the peptide fraction. (cdc.gov)
The serum peptide fraction, having cell-surface receptor ligand properties, conveys peripheral bioactivity in promoting endothelial cell inflammation, vasodilatory dysfunction and inhibiting angiogenesis. (cdc.gov)

To that end, a number of compounds have been evaluated for efficacy in vitro and in animal models, and several therapeutic modalities have been tested in the clinic. (usu.edu)
A number of pre-existing medicines were considered for re-purposing to treat Ebola because they have demonstrated efficacy against the virus in test tubes (in vitro). (who.int)

Mean serum titres varied significantly by age group: for diphtheria 0.24 IU/mL at age 2-4 years, 0.63 IU/mL at 7-8 years and 0.46 IU/mL at 11-12 years, and for tetanus 1.01 IU/mL, 2.63 IU/mL and 1.20 IU/mL respectively. (who.int)
They hypothesized that IP6 would significantly inhibit cell growth and increase the apoptotic rate of pancreatic cancer in vitro. (naturalnews.com)

In the general population aging is associated with immunological changes resulting in low grade inflammation and deterioration of the innate and adaptive immune system in the elderly ( 10 - 13 ). (frontiersin.org)

The cytotoxin neutralizing activity in sera from H. pylori-infected persons was mediated predominantly by the purified IgG fraction. (jci.org)

NAP-1-IgG was detected in 18 of 26 sera from normal humans. (nih.gov)
No antiviral drugs have been trialled in the treatment of humans with MVEV and there are few in-vitro or animal-model data. (mja.com.au)

Interestingly, a unique monocyte and T cell immune phenotypic profile predictive for age advancement was found within each group. (frontiersin.org)

In Dr. A.M. Shamsuddin's review of findings in Nutrition and Cancer vol. 55, he states that in addition to its role in cancer prevention and control of experimental tumor growth, progression and metastasis, IP6 possesses other significant benefits, such as the ability to enhance the immune system, function as a natural antioxidant, prevent calcification and kidney stone formation, lower elevated serum cholesterol, and reduce pathological platelet activity. (naturalnews.com)

studied the immune response to diphtheria and tetanus toxoid components of a combined diphtheria tetanus whole-cell pertussis/enhanced inactivated poliovirus vaccine, administered in a 3-dose schedule to Israeli infants at 2, 3.5 and 10 months of age and followed by a booster dose at the age of 8 years [3]. (who.int)

So far, no alternative tests applicable to such sera have been developed and systematically applied to vaccine testing. (forschung3r.ch)

The T cell immune phenotypic profile in blood donors was related to loss of T cell function, whereas the same set of markers were related to chronic antigen stimulation and immune senescence in HIV-negative individuals. (frontiersin.org)
While the monocyte and T cell immune phenotypic profile within the HIV-negative individuals reflected those observed in the combined three groups, a distinct profile related to immune dysfunction, was observed within blood donors and people with HIV. (frontiersin.org)

Classification of Hypersensitivity Reactions Allergic (including atopic) and other hypersensitivity disorders are inappropriate or exaggerated immune reactions to foreign antigens. (msdmanuals.com)

In various cellular system, to test the influence of immune complexes obtained from vaccinated cattle and pigs were established. (forschung3r.ch)

The immune serum used described and are now in various stages of testing (1-4). (cdc.gov)

The gut microbiome is intricately coupled with immune regulation and metabolism, but its role in Coronavirus Disease 2019 (COVID-19) is not fully understood. (rockefeller.edu)

In this study, we first examined the ovulation of Ciona intestinalis Type A by monitoring follicle rupture in vitro, identifying a novel mechanism of neuropeptidergic regulation of oocyte maturation and ovulation. (elifesciences.org)

Serum TIMP-1 may also serve as a marker of disease severity. (medscape.com)

This chapter evaluates the effects of fluoride on the gastrointestinal system (GI), the kidney, the liver, and the immune system, focusing primarily on new data that have been generated since the earlier NRC (1993) review. (nationalacademies.org)
As published in Journal of Surgical Research , June 2006, researchers reported that IP6 had previously been shown to have significant inhibitory effects against pancreatic cancer in vitro. (naturalnews.com)

T2-high severe asthma can be predicted to some extent from raised levels of F eNO , blood and sputum eosinophil counts, but serum IgE or serum periostin were poor predictors. (ersjournals.com)
In vitro fibroblasts from involved fascia produce increased levels of mRNA for collagen types I, III, and IV compared with adjacent dermal fibroblasts. (medscape.com)

We observed that age advancement in all three groups combined was associated with a monocyte immune phenotypic profile related to inflammation and a T cell immune phenotypic associated with immune senescence and chronic antigen exposure. (frontiersin.org)
The identified monocyte and T cell immune phenotypic profiles that were associated with age advancement, were strongly related to inflammation, chronic antigen exposure and immune senescence. (frontiersin.org)
Based on the review of the literature, there is no support for the concept that cold exposure depresses immune function. (researchgate.net)

HIV-associated immune dysfunction and inflammation have been suggested to contribute to this age advancement and increased risk of comorbidities. (frontiersin.org)

Using an ELISA for free NAP-1 with a detection limit of 200 pg/ml, we found no free NAP-1 in the 26 sera. (nih.gov)
Free anti-NAP-1-IgG autoantibody was found in 9 of 26 sera by direct ELISA. (nih.gov)
Some of these molecules can then be released extracellularly and be found in body fluids such as the serum. (hindawi.com)

Our data indicate efficient opsonization of ZIKV NS1-expressing CEM-NK R cell lines using ZIKV-immune but not ZIKV-naïve sera, a prerequisite of ADCC. (elsevierpure.com)

Anatomy35

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