• Her name was Henrietta Lacks, but scientists know her as HeLa. (worldcat.org)
  • In medical research, the most famous immortalized cell line, known as HeLa, was developed from cervical cancer cells of a woman named Henrietta Lacks. (wikipedia.org)
  • The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. (abcam.com)
  • Skloot is the author of the bestseller, The Immortal Life of Henrietta Lacks , which tells the story of how the cells of a woman who died nearly 60 years ago have launched medical breakthroughs and raised serious questions about science, ethics, research, race, class and history. (depauw.edu)
  • Named "HeLa" (short for Henrietta Lacks), the first "immortal" human cells grown in culture are still alive today. (depauw.edu)
  • Henrietta Lacks' cell line-known as HeLa cells-became and remain to this day a powerful tool for medical research. (nas.org)
  • When Henrietta Lacks was diagnosed with cancer in 1951, doctors took her cells and grew them in test tubes. (oprah.com)
  • Earlier this year, Henrietta Lacks' family settled a lawsuit against one of the Biotech firms that used her cells without their permission. (myfox8.com)
  • FILE - This April 26, 2007, file photo, shows the exterior of Thermo Fisher Scientific Inc., in Waltham, Mass. The estate of Henrietta Lacks sued a pharmaceutical company on Monday, Oct. 4, 2021, that it says has been selling cells that doctors at Johns Hopkins took from the Black woman from Maryland in 1951 without her knowledge or consent. (kristv.com)
  • Molecular docking studies indicated that all three complexes primarily bind to HSA in subdomain IIA with amino acid residues such as Arg218 and Lys199 which are located at the entrance of Sudlow's site I. The in vitro cytotoxicities of complexes 1-3 against HeLa cells showed promising anticancer activity (IC 50 = 3.5, 3.9 and 16.9 μM for 1 , 2 and 3 , respectively). (rsc.org)
  • Novel luteolin capped gold nanoparticles (AuNPL) were synthesized and their anticancer effect towards human cervical adenocarcinoma HeLa cells was investigated in vitro. (ac.rs)
  • Extracts of 6-month-old bud exhibited a significant in vitro anticancer activity against HeLa cancer cells with IC50 value of 56.8 µg/mL. (unboundmedicine.com)
  • Stabilization of quadruplex architecture by small molecules is thus emerging as a potential anticancer approach since it is thought to interfere with oncogenic expression and telomeric maintenance in cancer cells [ 11 - 13 ]. (hindawi.com)
  • Furthermore, in vitro experiments show that cancer cells can be effectively killed by anticancer drug ( paclitaxel ) released from the system, further indicating the promise of the controlled-release drug delivery system in cancer treatment. (rsc.org)
  • AuNPL cytotoxicity against HeLa, human malignant melanoma A375, and normal human keratinocytes HaCaT cells was tested by MTT cell survival assay, and their IC50 values were determined. (ac.rs)
  • The selectivity in the cytotoxic action of AuNPL on HeLa and A375 cancer cells compared with their cytotoxicity on normal keratinocytes HaCaT was observed. (ac.rs)
  • Cytotoxicity analyses of the drug delivery system based on human gastric cancer SGC-7901 and rat pheochromocytoma PC12 cells show low cytotoxicity and good biocompatibility. (rsc.org)
  • The generated series and parental molecule were tested in vitro for antiparasitic activity against Trypanosoma cruzi, Leishmania braziliensis, and L. amazonensis, and cytotoxicity using RAW cells. (lu.se)
  • Knockout cell lysate achieved by CRISPR/Cas9. (abcam.com)
  • Cell cycle analysis by flow cytometry with HeLa cells indicated that 1 and 2 induced cell cycle arrest in the G2/M phase while 3 induced arrest in the G0/G1 phase leading to cell death. (rsc.org)
  • n HeLa cells were demonstrated by flow cytometry. (ac.rs)
  • Cytoprotective properties of AuNPL towards HaCaT cells were examined by measuring the physiological and H2O2 induced intracellular reactive oxygen species (ROS) levels using flow cytometry. (ac.rs)
  • Here you had a time period, the '50s, where black people could not go into restaurants, they couldn't use the same bathrooms and water fountains as white people, yet this one black woman's cells were being used to develop the polio vaccine, which saved millions of white people's lives. (depauw.edu)
  • Known as HeLa cells, they became one of the world's most ubiquitous sources for medical research of everything from hormones, steroids and vitamins to gene mapping, in vitro fertilization, even the polio vaccine-all without the knowledge, must less consent, of the Lacks family. (kirkusreviews.com)
  • HeLa cells, as they are called, were essential in developing the polio vaccine. (crownpublishing.com)
  • The cells ultimately played a critical role in medical advancements, including developing the polio vaccine, in-vitro fertilization, and even COVID-19 vaccines. (kristv.com)
  • Also, genotoxicity of AuNPL in HaCaT cells was investigated by the single cell alkaline comet assay. (ac.rs)
  • The G-less cassette transcription assay is a method used in molecular biology to determine promoter strength in vitro. (wikipedia.org)
  • TUNEL assay on the WNV-Cp-transfected cells, indicating nuclear condensation (i) due to expression of capsid (j). (cdc.gov)
  • The effects of incorporating PE into PCL/GEL electrospun nanofibers were investigated regarding their physicochemical properties and in vitro biocompatibility. (mdpi.com)
  • Biocompatibility of composites was tested on HELF and HeLa cells and revealed no toxic effects. (nature.com)
  • it helped uncover the secrets of cancer and the effects of the atom bomb, and led to important advances like cloning, in vitro fertilization, and gene mapping. (oprah.com)
  • They have aided in the development of in-vitro fertilization, cloning, and gene mapping, and have helped us to better understand the workings of cancer and innumerable viruses. (crownpublishing.com)
  • Known as HeLa cells , they have been used in countless medical studies that paved the way for advancements in HIV, Polio, In vitro fertilization, gene mapping, and cancer treatments. (myfox8.com)
  • Access thousands of knockout cell lysates, generated from commonly used cancer cell lines. (abcam.com)
  • See here for more information on knockout cell lysates. (abcam.com)
  • Genital warts, which are a form of benign tumor of epithelial cells, are also caused by various strains of HPV. (wikipedia.org)
  • It is conceivable that this change may be related to that which enables the cells of a compact tumor to become invasive. (onclive.com)
  • The case involved cells derived from a cancerous cervical tumor in a woman who died from the disease in 1951. (nas.org)
  • Her inadvertent legacy is that her uterine tumor cells proved to be the first human cells that kept growing and reliably reproducing in vitro. (nas.org)
  • Modified versions of AAVs range from genetic alterations of the viral cap gene to conjugation of proteins such as streptavidin or antibodies to promote interaction with specific cells. (nature.com)
  • Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. (abcam.com)
  • It induces apoptosis in HeLa cells (100µM) where it down regulates global gene expression. (enzolifesciences.com)
  • Moreover, AAV9-NEM localized strongly in vivo to cells lining the calcified trabecular bone and transduced primary murine osteoblasts in culture, while WT AAV9 transduced undifferentiated bone marrow stromal cells as well as osteoblasts. (nature.com)
  • SIRT2 deacetylates lysine-40 of alpha-tubulin both in vitro and in vivo. (nih.gov)
  • NCT demonstrated antiviral activity against adenovirus in vitro and in vivo. (arvojournals.org)
  • The in-vitro studies suggested that qualitatively similar effects may be induced by in- vivo exposure to electromagnetic fields at these frequencies. (cdc.gov)
  • It is a potent inhibitor of histone acetyltransferases (HATs) p300 (IC 50 =7µM) and PCAF (IC 50 =5µM) both in vitro and in vivo . (enzolifesciences.com)
  • Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
  • Os estudos in vitro estão livres da complexidade e fatores de confusão próprios dos modelos in vivo e ensaios clínicos. (bvsalud.org)
  • As your recommendaion, the Jurkat cells treated with anti Fas antibody to induce cleavage is positive control. (abcam.com)
  • Materials and Methods Macrophages derived from the THP-1 cell line were differentiated with interleukin-4 to induce a 'wound-healing'-like phenotype, and treated with various dilutions of Arnica m . centesimal (100 times) dilutions (2c, 3c, 5c, 9c, and 15c) or control solvent for 24 hours. (thieme-connect.com)
  • Macrophages derived from the THP-1 cell line were differentiated with IL-4 to induce a 'wound-healing'-like phenotype. (thieme-connect.com)
  • The results indicated that porous spherical CaCO 3 microparticles may induce oxidative damage to Hela cells. (scirp.org)
  • The first 'immortal' human cells grown in culture, they are still alive today, though she has been dead for more than sixty years. (worldcat.org)
  • She was a poor Southern tobacco farmer who worked the same land as her slave ancestors, yet her cells--taken without her knowledge--became one of the most important tools in medicine: The first immortal human cells grown in culture, which are still alive today, though she has been dead for more than sixty years. (booksamillion.com)
  • And though the cells had launched a multimillion-dollar industry that sells human biological materials, her family never saw any of the profits. (booksamillion.com)
  • Granulysin is an antimicrobial peptide (AMP) expressed by human T-lymphocytes and natural killer cells. (uni-ulm.de)
  • HeLa cells are human epithelial cells from a fatal cervical carcinoma. (abcam.com)
  • This cell line, dubbed the HeLa cell line after Lacks' name, has since become one of the most widely used human cell lines in biologic research over the past half century, with the cell lines having been used in over 100,000 scientific publications on PubMed alone. (onclive.com)
  • This new work tested the effects of Arnica m. homeopathic dilutions on human macrophages, key cells in tissue defence and repair. (thieme-connect.com)
  • The uptake properties of the system by SGC-7901 and human cervical carcinoma HeLa cells demonstrate their great potential for applications in drug delivery. (rsc.org)
  • of Memphis) demonstrates that for every human cell put under a microscope, a complex life story is inexorably attached, to which doctors, researchers and laboratories have often been woefully insensitive and unaccountable. (kirkusreviews.com)
  • Cell types included resting human peripheral lymphocytes, LN71 or RT2 glioma cells, Chinese-hamster-ovary cells, HeLa cells, and mouse spermatozoa. (cdc.gov)
  • Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
  • A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)
  • Most crude systems, such as HeLa nuclear extracts, are used because they contain low amounts of contaminating GTP that lead to background transcription and may occasionally cause random transcription to read through the G-less cassette. (wikipedia.org)
  • Typical nuclear staining was observed with the cells expressing WNV-CpWT (e) compared to the cells expressing WNV-Cp-DJY (g). (cdc.gov)
  • The 2 cell types possess volumes, nuclear cross-sections, plating efficiencies, and generation times which are identical within experimental error, but display widely different cross-sectional areas, suggesting that the basic change occurs in the cell surface," wrote the authors of the study. (onclive.com)
  • In fact, an estimated 50 million metric tons of HeLa cells have been grown, with a combined weight, says Skloot, of the equivalent of more than 150 Empire State Buildings. (depauw.edu)
  • One scientist estimates that if you could pile all the HeLa cells ever grown onto a scale, they'd weigh more than 50 million metric tons-the equivalent of at least 100 Empire State Buildings. (oprah.com)
  • If you could pile all HeLa cells ever grown onto a scale, they would weigh more than fifty million metric tons-more than a hundred Empire State Buildings. (crownpublishing.com)
  • Using activity-guided purification of tRNA ligase from HeLa cell extracts, we identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex. (nih.gov)
  • To test whether Cx32 is required to propagate an oxidative stress signal to neighboring cells, the team generated an in vitro co-culture system. (genengnews.com)
  • The discovery of the immortal, ever-replicating HeLa cell line in 1952 has led to several breakthroughs in biomedical research for countless diseases including cancer, AIDs, and polio. (onclive.com)
  • She died without knowing that her cells would become immortal -the first to grow and survive indefinitely in culture. (crownpublishing.com)
  • However, the identity of the woman who contributed these virtually immortal cells remained unknown until decades after her death. (myfox8.com)
  • Cx32-deficient and Cx32-expressing HeLa cells were stimulated with the reactive metabolite of TAA (thiacetamide sulfoxide, TASO), either in the absence of presence of free radical scavengers. (genengnews.com)
  • The reactive oxygen species (ROS), glutathione (GSH), carbonyl content in proteins (CCP), DNA-protein crosslinks (DPC) and cell viability were measured. (scirp.org)
  • IC50 concentration of AuNPL exerted cytoprotective effect against HaCaT cells by the significant reduction of the physiological intracellular ROS level. (ac.rs)
  • Results Arnica montana at the 2c dilution (final concentration of sesquiterpene lactones in cell culture = 10 −8 mol/L) significantly stimulated the expression of three genes which code for regulatory proteins of the extracellular matrix, namely FN1 (fibronectin 1, % increase of 21.8 ± standard error of the mean 4.6), low-density lipoprotein-receptor-related protein 1 (% increase of 33.4 ± 6.1) and heparan sulphate proteoglycan 2 (% increase of 21.6 ± 9.1). (thieme-connect.com)
  • In vitro, NCT demonstrated concentration-dependent direct inactivation of all ocular Ad serotypes tested. (arvojournals.org)
  • Results showed that with the exposure concentration increase, ROS and CCP in Hela cells presented a significant increase but GSH contents in Hela cells and cell viability showed a significant decrease respectively compared with the control. (scirp.org)
  • If you require a native form of the protein please use the live cell version - found here . (abcam.com)
  • d, Protein expression by Western blot analysis, of WNV-Cp expression in HeLa cells. (cdc.gov)
  • Subcellular location of WNV-Cp protein, in HeLa cells transfected with pcWNV-CpWT (e,f) or pcWNV-Cp-DJY (g,h) plasmids. (cdc.gov)
  • Wild-type (WT) AAV serotypes exhibit different tissue tropism due to different capsid protein sequences or variable regions interacting with disparate cell surface receptors, and thus can be utilized for different target tissues 3 . (nature.com)
  • The investigators moved on to think about evaluating the therapeutic potential of pharmacologically inhibiting Cx32, using 2-aminoethoxy-dipenyl-borate (2APB), a compound previously shown to transiently inhibit Cx32 gap junctions in vitro, and which they confirmed was specific to the Cx32 gap junction protein. (genengnews.com)
  • This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. (cancerindex.org)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • In addition, FN1, unlike other candidate genes, was upregulated in cells treated with higher dilutions/dynamisations (3c, 5c, and 15c) of Arnica m. (thieme-connect.com)
  • 3 While most cancer cell samples tended to divide a few times and die before any notable research could be conducted, investigators found that Lacks' cells continued to divide indefinitely, provided they were given the right amount of nutrients. (onclive.com)
  • Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
  • In line with this, RBPJL is able to fully reconstitute transcriptional repression at Notch target genes in cells lacking RBPJ. (uni-ulm.de)
  • The adaptive response depends on p53 , as observed in cell experiments. (go.jp)
  • RNA samples from cultured cells were analysed by real-time quantitative polymerase chain reaction in five separate experiments. (thieme-connect.com)
  • Prepared colloids show protrombotic activity and cause plasma coagulation in in vitro experiments. (nature.com)
  • 3,4 There is, however, one pressing issue with the discovery that has negatively impacted the relationship between the medical community and with patients of color: Lacks' cervical cancer cells were collected without her or her family's consent. (onclive.com)
  • Although the collection of Lacks' cells is a subject that continues to be surrounded by controversy to this day, it's difficult to deny the progress that has been made in medical research as a byproduct of this discovery, in the field of cancer and beyond. (onclive.com)
  • The National Institutes of Health announced on August 7 that it had reached an agreement with members of the Lacks family that would give them rights over a widely used line of cultured cells. (nas.org)
  • She reconstructs what she can of the actual mortal life of Henrietta, mixes it with a memoir of her own quest to turn up the facts, adds in a bit of the scientific background, and gives generous space to the grievances of current members of the Lacks family, who generally feel disappointed that they didn't see any monetary benefit from the HeLa cells. (nas.org)
  • With this book, we too become immersed in the story of the Lacks family, and are shocked to discover that Henrietta's husband and children did not find out about her "immortality," or the enormous profits her cells had generated, until more than twenty years after her death, when scientists investigating HeLa began using blood samples from her family in research without informed consent. (crownpublishing.com)
  • They soon made the shocking discovery that, unlike most cells, Lacks cells didn't die like most people. (myfox8.com)
  • One of the older innovations to emerge from the discovery were methods for testing for cancerous cells that proved to be so reliable, they continue to be used by investigators in the modern age. (onclive.com)
  • 5 Two methods of quick and simple plating of single HeLa cells that results in the growth and formation of colonies from individual cells were detailed in a paper that was published in 1956. (onclive.com)
  • Based on these findings, investigators concluded that these methods would be useful for examining mammalian cell growth, physiology, genetics, and biochemistry in addition to examining how cells respond to drugs, viruses, and high-energy radiation. (onclive.com)
  • Although the research that emerged from the discovery of the HeLa cell line has helped to prevent 4.5 billion global infections and 10.3 million global deaths, the unethical and controversial nature of their discovery has raised issues with regard to privacy and consent in underrepresented patient populations. (onclive.com)
  • In honor of Black History Month, Markman looks back on the progress that has been made through the HeLa cell line, incidents that have contributed to a lack of trust in the medical community, and efforts that need to be made to ensure that the rights of underserved patient populations are maintained in clinical cancer trials. (onclive.com)
  • Even today, HeLa is the most widely used cell line in labs worldwide, bought and sold by the billions. (crownpublishing.com)
  • After learning about the HeLa cell line in high school, Rebecca Skloot became consumed by curiosity about the woman behind the cells. (crownpublishing.com)
  • AuNPL exerted their cytotoxic activity against HeLa cells through accumulation of the cells in the subG1 phase of the cell cycle, inducing the apoptotic cell death mediated by the activation of caspase-3 − 8, and − 9. (ac.rs)
  • To determine whether N -chlorotaurine (NCT) demonstrates antiviral activity against adenovirus (Ad) in vitro and in the Ad5/NZW rabbit ocular model. (arvojournals.org)
  • The in vitro activity of NCT was evaluated by incubating different Ad serotypes with several concentrations of NCT for 1 hour and determining the reduction in Ad titers. (arvojournals.org)
  • In an effort to further understanding of the effects of radiofrequency (RF) radiation on somatic cell mitotic activity and germ cell function, cell suspensions were exposed to 27 megahertz (MHz) or 2,450MHz radiation under isothermal conditions in-vitro. (cdc.gov)
  • BACKGROUND: Thylakoid-stabilised emulsions have been reported to possess satiety-promoting effects and inhibit pancreatic lipase-colipase activity in vitro, which prompted the investigation of their interfacial properties. (lu.se)
  • Numerous studies currently suggest that quadruplex formation in this overhang alters the structure and function of telomeres, inducing a damage response and rapid apoptosis in particular in cancer cells [ 14 - 24 ]. (hindawi.com)
  • But her cells are still among the most widely used in labs worldwide-bought and sold by the billions. (oprah.com)
  • About 90% of cervical cancer cases are squamous cell carcinomas, 10% are adenocarcinoma, and a small number are other types. (wikipedia.org)
  • Locally advanced squamous cell carcinomas of the head and neck, (excluding lip) in combination with concurrent chemoradiation. (nih.gov)
  • Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells. (nih.gov)
  • Biology of Stem Cells and the Molecular Basis of the Stem State. (iiarjournals.org)
  • The open-circuit voltages of organic solar cells (OSCs) based on Y6-D2, Y6-D3, and Y6-D4, except for Y6-D1, may be higher than those of OSCs based on the Y6 acceptor because of their higher energy levels of lowest unoccupied molecular orbital (LUMO). (bvsalud.org)
  • The Notch signaling pathway is an evolutionary conserved signal transduction cascade present in almost all tissues and is required for embryonic and postnatal development, as well as for stem cell maintenance, but it is also implicated in tumorigenesis including pancreatic cancer and leukemia. (uni-ulm.de)
  • RBPJL is specifically expressed in the exocrine pancreas, whereas it is mostly undetectable in pancreatic tumour cell lines. (uni-ulm.de)
  • Gran1 was selectively internalized by macrophages, the major host cell of Mtb , and restricted the proliferation of the pathogen. (uni-ulm.de)
  • Studies were also conducted on liposome permeability, and a mathematical model was used to calculate radiation energy absorption in mammalian cell models. (cdc.gov)
  • The first method utilizes irradiated, non-multiplying feeder cells to condition the medium, while the second method utilizes a similar, but gentler, strategy that involves plating bacteria on semisolid nutrient media. (onclive.com)
  • Though those cells have done wonders for science, Henrietta-whose legacy involves the birth of bioethics and the grim history of experimentation on African-Americans-is all but forgotten. (oprah.com)
  • This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
  • This radiation-adaptive response in whole animals differs from that in cells observed several hours to several days (at most) later. (go.jp)
  • Effects of 27 MHz radiation on somatic and germ cells. (cdc.gov)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • The absence of GTP in vitro results in transcription being prematurely terminated at the first guanine residue in the sense strand following the cassette. (wikipedia.org)
  • Conclusion The results support evidence that the extracellular matrix is a potential therapeutic target of Arnica m., with positive effects on cell adhesion and migration during tissue development and healing. (thieme-connect.com)
  • The recovery of blood-forming stem cells, determined as endogenous spleen colonies, was markedly stimulated by priming irradiation. (go.jp)
  • The reduction in bone marrow death seems chiefly due to the stimulated recovery of blood-forming stem cells. (go.jp)
  • The stem cells might produce an unknown factor(s) that contributes to acquired radioresistance. (go.jp)
  • Stem Cells and Cancer. (iiarjournals.org)
  • Regulatory Networks in Stem Cells. (iiarjournals.org)
  • Those cells led to breakthroughs in everything from Parkinson's to polio. (oprah.com)
  • Among immunodeficient individuals, toxoplasmosis most often occurs in those with defects of T-cell-mediated immunity, such as those with hematologic malignancies, bone marrow and solid organ transplants, or acquired immunodeficiency syndrome ( AIDS ).In most immunocompetent individuals, primary or chronic (latent) T gondii infection is asymptomatic. (medscape.com)
  • Henrietta's family did not learn of her immortality until more than twenty years after her death, when scientists investigating HeLa began using her husband and children in research without informed consent. (booksamillion.com)
  • And for some reason, Henrietta's cells just never died. (depauw.edu)
  • There's no way of knowing exactly how many of Henrietta's cells are alive today. (oprah.com)
  • She spent years winning the trust of Henrietta's daughter Deborah, who longed to know more about her mother and to better understand the science behind her cells, which often seemed more like science fiction. (crownpublishing.com)
  • It was demonstrated that expression of interleukin‑8 (IL‑8) mRNA was significantly upregulated in Gem‑R PaCa cells by cDNA microarray and RT‑qPCR analyses. (cancerindex.org)
  • One of the reasons for this is the acquisition of Gem resistance (Gem‑R). To elucidate the mechanism of Gem‑R, two Gem‑R PaCa cell lines were established from AsPC‑1 and MIA PaCa‑2 cells. (cancerindex.org)
  • According to the AP, Johns Hopkins said it never sold or profited from the cell lines. (kristv.com)