Vitro flow cytometry cell cycle. Medical search. Frequent questions

The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
Our results suggest that BRCA1 overexpression sensitizes cancer cells to lovastatin via regulation of cyclin D1-CDK4-p21WAF1/CIP1 pathway, which will provide an innovative experimental framework to study control of breast cancer cell proliferation. (nih.gov)
In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (nih.gov)
Treatment of OMM2.5 MUM cells with ACY-1215 resulted in a significant ( p = 0.0001), dose-dependent reduction in cell survival and proliferation in vitro , and in vivo regression of primary OMM2.5 xenografts in zebrafish larvae. (biorxiv.org)
When genetically susceptible individuals are exposed to infection, stress or trauma, cutaneous dendritic cells are activated to produce tumor necrosis factor (TNF)-α and interleukin (IL)-23, which subsequently stimulate the proliferation and differentiation of proinflammatory T cells. (nature.com)
Further, the results of miR-4755-5p mimic transfection confirmed that under the action of NaF, miR-4755-5p overexpression reduced Cyclin D1 protein expression within osteoblasts and further inhibited cell proliferation and activation. (fluoridealert.org)
The inhibitory effect of MicroRNA-16-5p on the proliferation, invasion, cell cycle and apoptosis of glioma cells was evaluated by the cell proliferation, invasion and flow cytometry. (ijpsonline.com)
Cell viability, proliferation, DNA damage response and apoptosis were investigated using immunofluorescence staining and immunoblotting. (bmj.com)
Results Gemcitabine treatment caused DNA damage, cellular stress, inhibited proliferation and activated cell cycle check-point sensors and induced apoptosis as shown by increased expression of g-histone H2AX, p-JNK, Chk-1/Chk-2, cleaved forms of PARP and caspase-3 in the asynchronous cells in a dose dependent manner. (bmj.com)
As a result, the proliferation and in vitro AMH and oestrogen production of the cells were decreased at post-exposure 24h. (bmj.com)
In the cells synchronized at S phase gemcitabine significantly inhibited DNA synthesis and blocked their proliferation. (bmj.com)
To investigate the effects of silibinin on the growth of oral cancer cells, cell proliferation and anchorage-independent colony formation tests were conducted on YD10B and Ca9-22 oral cancer cells. (jcancer.org)
Silibinin effectively suppressed YD10B and Ca9-22 cell proliferation and colony formation in a dose-dependent manner. (jcancer.org)
Methods: Human NET cell lines BON1, QGP-1, and NCI-H727 were treated with trametinib or SCH772984, alone and in combination with ribociclib, to assess cell proliferation, cell cycle distribution, and protein signaling using cell proliferation, flow cytometry, and Western blot assays, respectively. (uni-muenchen.de)
Endothelial cell lineage tracing showed that BNP directly stimulated the proliferation of resident endothelial cells via NPR-A binding and p38 MAP kinase activation. (elifesciences.org)
BNP also stimulated the proliferation of WT1 + epicardium-derived cells but only in the hypoxic area of infarcted hearts. (elifesciences.org)
The origin of new endothelial cells (i.e. resident or infiltrating) as well as the underlying mechanism leading to their proliferation (partial endothelial-to-mesenchymal transition [EndMT] or not) have long been debated. (elifesciences.org)
Additionally, GEM or GEM+CDDP at low concentrations dramatically facilitated the proliferation, migration, and invasion of tumor cells in vitro . (researchsquare.com)
Poisson-event-based analysis of cell proliferation. (swansea.ac.uk)
While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. (biomedcentral.com)
To this end, we overexpressed and inhibited gga-miR-155 in an MDV-transformed cell line using mimics and inhibitors respectively, and analyzed their growth, proliferation, apoptosis and invasiveness to explore the possible role of gga-miR-155 in MDV-mediated tumorigenesis. (biomedcentral.com)
Furthermore, we predicted and demonstrated that Retinoid Acid Receptor-Related Orphan Receptor Alpha (RORA) is one of the targets of gga-miR-155, and the gga-miR-155 regulated the proliferation, cell cycle, apoptosis and invasiveness of MSB1 cells by targeting RORA. (biomedcentral.com)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

Cell Counting Kit (CCK)-8 assay and flow cytometry were employed to identify cell viability and apoptosis/cell cycle, respectively. (techscience.com)
The over expression of MicroRNA-16-5p can inhibit the growth and invasion of the glioma cell line, promote the apoptosis and delay the cell cycle. (ijpsonline.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Introduction/Background We aimed to compare gemcitabine vs. cisplatin in terms of DNA damage response, viability/apoptosis of malignant granulosa cells. (bmj.com)
In addition, ebastine treatment exerted obvious effects on cell cycle arrest, metastasis inhibition, apoptosis and autophagy induction both in vitro and in vivo . (ijbs.com)
Extensive support for basic and complex cell cycle analysis and apoptosis assays. (embl.org)
Flow cytometry was used to examine apoptosis, cell cycle distribution, and accumulation of reactive oxygen species (ROS). (jcancer.org)
Moreover, it induced cell cycle arrest in the G0/G1 phase, apoptosis, and ROS generation in these cells. (jcancer.org)
Silibinin considerably reduced the development of oral cancer cells by inducing apoptosis, G 0 /G 1 arrest, ROS generation, and activation of the JNK/c-Jun pathway. (jcancer.org)
Functional studies showed that LIPH-4 promoted the growth, mediated cell cycle progression and inhibited apoptosis in ESCC cells in vitro , and promoted tumor growth in mice. (researchsquare.com)
The in vitro MNT allows the detection of both clastogen and aneugens and it can simultaneously detect mitotic delay, apoptosis, chromosome breakage, chromosome loss and non-disjunction. (cyprotex.com)
Tumor and endothelial cell functions, apoptosis, cell cycle analysis, as well as bone marrow derived cells (BMDCs) mobilization, were evaluated with transwell, MTT or flow cytometry analysis in vitro , respectively. (researchsquare.com)
Cell-cycle arrest, activation of associated apoptotic proteins, and inhibition of apoptosis were also observed in tumor cells. (researchsquare.com)
In vitro , we assessed NF-κB activity and apoptosis in response to pomalidomide alone, gemcitabine/nab-paclitaxel, or combination of pomalidomide and gemcitabine/nab-paclitaxel in human pancreatic cancer cell lines (PANC-1 and MIA PaCa-2). (oncotarget.com)
In pomalidomide and gemcitabine/nab-paclitaxel group, gemcitabine/nab-paclitaxel-induced NF-κB activation was inhibited and apoptosis was enhanced in comparison with those in the other groups both in vitro and in vivo . (oncotarget.com)
Students of apoptosis and other types of cell death hypothesize that virtually all cells are genetically programmed to self-destruct. (nhbs.com)
S.W. Sherwood and R.T. Schimke, Cell Cycle Analysis of Apoptosis Using Flow Cytometry. (nhbs.com)
A.J. McGahon, S.J. Martin, R.P. Bissonnette, A. Mahboubi, Y. Shi, R.J. Mogil, W.K. Nishioka, and D.R. Green, The End of the (Cell) Line: Methods for the Study of Apoptosis In Vitro. (nhbs.com)
Using each particle's IC(50) cell viability in a BEAS-2B cell model, pristine nanoclay exposure caused acute loss of monolayer integrity, decreased metabolism, and increased apoptosis. (cdc.gov)
Three different ONCs, however, displayed minimal loss to monolayer integrity despite coating type-dependent differences in apoptosis induction and decreased cell metabolism. (cdc.gov)
We have used human Mono Mac 6 cells to investigate the influence of RF EMFs in vitro on cell cycle alterations and BrdU uptake, as well as the induction of apoptosis and necrosis in human Mono Mac 6 cells, using flow cytometry after exposure to a 1,800 MHz, 2 W/kg specific absorption rate (SAR), GSM-DTX signal for 12 h. (monocyte.eu)
No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after RF EMF exposure compared to sham or incubator controls. (monocyte.eu)
RF EMF radiation did not induce cell cycle alterations or changes in BrdU incorporation or induce apoptosis and necrosis in Mono Mac 6 cells under the exposure conditions used. (monocyte.eu)

Changes in density of viable cells after exposure were measured using the MTT assay. (niom.no)
HPMA exposure resulted in reduced SDH activity (MTT assay), altered cell growth pattern and increased cell death in a dose dependent manner in all investigated cell lines. (niom.no)
Flow cytometry, caspase-3 activity assay in treated monolayers/spheroids and also fluorescence staining and DNA fragmentation in treated monolayers demonstrated apoptotic death mode. (irost.org)
3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, acridine orange, and monodansylcadaverine staining and flow cytometry were performed. (hindawi.com)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)
They had appropriate birth weight and all cytometry [ 10 ] or polymerase chain reac- had a dietetic cause for their condition start- tion assay [ 11 ]. (who.int)

It also has applications in cell sorting, cell-cycle analysis, immunology, and cell viability studies. (grandviewresearch.com)
In agreement, pharmacological inhibition of MITF signaling with ML329, significantly reduced OMM2.5 cell survival and viability in vitro ( p = 0.0001) and in vivo ( p = 0.0006). (biorxiv.org)
Cell viability was detected by CCK-8 method. (fluoridealert.org)
Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
Results: Trametinib and SCH772984, alone and in combination with ribociclib, significantly reduced NET cell viability and arrested NET cells at the G1 phase of the cell cycle in all three cell lines tested. (uni-muenchen.de)

In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. (irost.org)
In addition, trametinib also caused subG1 events and apoptotic PARP cleavage in QGP1 and NCI-H727 cells. (uni-muenchen.de)
Exclusion of necrotic and late apoptotic cells from analysis reduces false positives for genotoxicity. (cyprotex.com)
P.J. Fraker, L.E. King, D. Lill-Elghanian, and W.G. Telford, Quantification of Apoptotic Events in Pure and Heterogeneous Populations of Cells using the Flow Cytometer. (nhbs.com)
L.T. Bemis, F.J. Geske, and R. Strange, Use of the Yeast Two-Hybrid System for Identifying the Cascade of Protein Interactions Resulting in Apoptotic Cell Death. (nhbs.com)
However, in the positive control cells treated with gliotoxin and PMA (phorbol 12 myristate-13 acetate), a significant increase in apoptotic and necrotic cells was seen. (monocyte.eu)
Flow cytometric estimation of the apoptotic marker CD95 in peripheral neutrophils, lymphocytes and monocytes was done for 18 infants with non-oedematous protein energy malnutrition (PEM) and 12 oedematous ones, on hospital admission and after supervised nutritional rehabilitation, and compared with 12 matched controls. (who.int)

In-vitro-treatment with Lonafarnib leads to a dose-dependent G2-arrest of glioblastomacells, lungcancer- and fibrosarcomacells. (uni-marburg.de)
Flavopiriol blocks the ATP-binding site of cyclin-dependent cinases and leads to a G1- respectively G2-cellcycle arrest in vitro. (uni-marburg.de)
The combined treatment of Flavopiriol and Lonafarnib in BG1-cells showed a slightly increased cell cycle arrest in the subG1-fraction in selected concentrations. (uni-marburg.de)
Therefore, compared to normal p53-proficient cells, p53-defective cells are more reliant on MK2 activity, which drives an alternative cell cycle checkpoint pathway that stabilizes the CKI inhibitors p27 Kip1 and Gadd45α in order to maintain G 1 /S and G 2 /M arrest after certain types of DNA damage 16 , 18 . (nature.com)
We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (nih.gov)
However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (nih.gov)
We address this question using CDK4/6 inhibitors, which arrest cells in G0/G1 and are licensed to treat advanced HR+/HER2- breast cancer. (bvsalud.org)
Objective: To investigate the fate and underlying mechanisms of G2 phase arrest in cancer cells elicited by ionizing radiation (IR). (bvsalud.org)
Results: Tumor cells suffered severe DNA damage and underwent G2 arrest after IR treatment. (bvsalud.org)
Exposure of the cells to gemcitabine at G2/M transition abolished the progression of mitosis, caused mitotic arrest and failure to exit mitosis as evidenced by the inhibition of Cyclin B degradation and absence of de-phosphorylation of cdc-2 at Tyr 15 residue. (bmj.com)
Indeed, OEO/thymol increased the Reactive Oxygen Species (ROS) level leading to mitochondrial membrane potential (MMP, ΔΨm) loss, caspase-3 activation and DNA damage caused S-phase cell cycle arrest. (irost.org)
A cell cycle arrest in early S-phase and in G 0 /G 1 -phase was also found after exposure to cisplatin and sunitinib malate, in isolation, respectively. (hindawi.com)
Hypoxic induction was confirmed using flow cytometric analysis, which showed cell cycle arrest starting at a 200 µM concentration of cobalt chloride. (molvis.org)
Flow cytometry allows multiparametric analysis of compound effects including cytostasis, cellular death and cell cycle arrest in addition to micronucleus formation. (cyprotex.com)
Moreover, pomalidomide induced G0/G1 cell cycle arrest and suppressed angiogenesis. (oncotarget.com)

Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). (irost.org)
The aim of this paper is to analyse sunitinib malate in vitro ability to enhance cisplatin cytotoxicity in T24, 5637, and HT1376 human urinary bladder-cancer cell lines. (hindawi.com)
Hypoxia of CEC was induced by exposing the cells to cobalt chloride (Sigma-Aldrich, St. Louis MO) in serum media, as described previously [ 12 , 13 ], and the induction was confirmed by cytotoxicity and cell cycle analysis. (molvis.org)
These results suggest the type of quaternary ammonium coating and incineration status largely impacts mechanism of cytotoxicity, cell metabolism, and the recovery ability of the exposed bronchial epithelial cell monolayer. (cdc.gov)
NK cells are specialized effectors of the innate immune system that destroy their targets by antibody-dependent cell-mediated cytotoxicity, have prominent antitumor effects, and are potent killers of virally infected cells. (medscape.com)

Scientific Methods: In vitro growth inhibition assays were completed with AHCC alone and in combination with PLD in panel of human cancer cell lines and findings confirmed in vivo in an ovarian cancer xenograft mouse model. (researchgate.net)
Methods: Human melanoma A375 and 92-1 cells were treated with X-rays radiation or Aurora A inhibitor MLN8237 (MLN) and/or p21 depletion by small interfering RNA (siRNA). (bvsalud.org)
Cell Death provides a current and comprehensive collection of methods for the study of cell death. (nhbs.com)
J.F.R. Kerr, G.C. Gobe, C.M. Winterford, and B.V. Harmon, Anatomical Methods in Cell Death. (nhbs.com)
An integrated high-throughput in vitro screening strategy, using high content imaging and traditional in vitro methods, represents a rapid pulmonary epithelial toxicity assessment approach to prioritize ENMs for further evaluation and serves to inform 'prevention-by-design' material development strategies. (cdc.gov)
METHODS: Blood and vaginal lavage samples were collected from 18 pig-tailed macaques to evaluate immune changes over reproductive cycles, and from 5 additional animals undergoing repeated vaginal exposures to simian HIV (SHIV). (cdc.gov)

It is used for cell counting & sorting, protein engineering, and detection of the biomarker by passing them by an electronic detection equipment and suspended cells in a fluid stream. (grandviewresearch.com)
Additionally, the mRNA and protein expression of cyclin D1, cyclin-dependent kinase 4 (CDK4) and retinoblastoma protein (pRb), was further down-regulated under exposure to lovastatin in condition of BRCA1 overexpression, but the expression of p21WAF1/CIP1, a cyclin-dependent kinase inhibitor (CDKI), was further up-regulated, both in vitro and in vivo detected with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis. (nih.gov)
Progress in understanding tumor stromal biology has been constrained in part because cancer-associated fibroblasts (CAF) are a heterogeneous population with limited cell-type-specific protein markers. (aacrjournals.org)
More importantly, we found that IPMK interacted with AMPK and functioned as a positive regulator of AMPK protein in osteosarcoma cells. (ijbs.com)
Establishment of clonal cell lines carrying fluorescent protein-tagged genome-edited genes for 4D life cell imaging, and assessment of protein interactions and concentrations during mitosis. (embl.org)
Studies characterising the immune response in patients with COVID-19 show that the correlates of protection in infected individuals are T-cell responses against the SARSCoV-2 spike protein and neutralising titres of immunoglobin G and immunoglobin A antibodies. (bvsalud.org)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

BACKGROUND/AIM: P21 is a cyclin-dependent kinase inhibitor regulating the cell cycle as a tumor suppressor. (bvsalud.org)

These findings establish a mechanism for co-targeting DNA damage-induced cell cycle checkpoints in combination with repair of cisplatin-DNA lesions in vivo using RNAi nanocarriers, and motivate further exploration of ASL as a generalized strategy to improve cancer treatment. (nature.com)
Major Findings: The in vitro growth inhibition assays demonstrated additive activity when AHCC is co-administered with PLD. (researchgate.net)
Our findings provide evidence that ACY-1215 and ML329 are efficacious against growth and survival of MUM cells and are potential therapeutic options for MUM. (biorxiv.org)
Altogether, our findings lay a foundation for improving the efficiency of hematopoietic differentiation and generating in vivo functional HSC-like cells from mouse PSCs for clinical application. (biomedcentral.com)
Our findings were subsequently tested by in vitro experiments, where putative early and later-stage DN1 progenitors from the thymus were purified and their pro- gression into DN2 was measured. (lu.se)
FINDINGS: Macaques cycle-phases were associated with fluctuations in systemic immune properties and a type-1 inflammatory T cell response with corresponding CCR5+ memory CD4 T cell (HIV target cell) infiltration into the vaginal lumen at the late luteal phase. (cdc.gov)

Cellcycle-analysis was applied via flow cytometry. (uni-marburg.de)
In 2018, BioLegend introduced TotalSeq™ antibody-oligonucleotide conjugates for use in single cell proteogenomics analysis. (wikipedia.org)
Flow cytometry involves the analysis of the optical and visible light characteristics of a single particle (e.g. cells, nuclei, chromosomes) throughout their passage within a slender, specifically printed liquid stream. (grandviewresearch.com)
Cell cycle analyses were performed using flow-cytometry and multicycle analysis software (Phoenics Flow Systems, San Diego, CA, USA). (niom.no)
Cell cycle analysis of in vitro cultured goral (Naemorhedus caudatus) adult skin fibroblasts. (druglib.com)
Cells in the lymphocyte gate were used for analysis. (thermofisher.com)
Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis. (thermofisher.com)
Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Cell cycle analysis was carried out using flow cytometry. (bmj.com)
The Flow Cytometry Core Facility offers access to high-end FACS analysis and state-of-the-art cell sorting capabilities, actively providing scientists with adaptive setups and dedicated support. (embl.org)
We offer a wide range of flow cytometric techniques, hands-on service, data analysis, and tailored training sessions for users inside and outside EMBL. (embl.org)
Flow cytomery applications used by EMBL researchers: Cell cycle analysis and Phenotyping of immune cells. (embl.org)
Full spectral cytometry for multi-parametric analysis of cell populations with high autofluorescence and/or low levels of expression of key biomarkers. (embl.org)
Flow Cytometry data analysis workshops at EMBL-Heidelberg and EMBL-Rome. (embl.org)
Complex multi-color analysis of cell populations based on light scatter and fluorescent probes content by conventional and spectral cytometry. (embl.org)
We offer to our researchers' advice and dedicated training sessions in the use of flow cytometry, instrument operation, and post-acquisition data analysis. (embl.org)
Induction of hypoxia was confirmed by flow cytometric analysis and the levels of SIRT1 were noted in a hypoxic condition as well in the cells after blocking SIRT1 activity using sirtinol. (molvis.org)
A western blot analysis demonstrated the use of trametinib alone and trametinib in combination with ribociclib to decrease the expression of pERK, cMyc, Chk1, pChk2, pCDK1, CyclinD1, and c-myc in a time-dependent manner in NCI-H727 and QGP-1 cells. (uni-muenchen.de)
Monitoring of cytostasis and exclusion of cytostatic cells from micronucleus analysis also helps to reduce false negative results. (cyprotex.com)
Representative data for micronucleus formation and cell cycle analysis. (cyprotex.com)
Cells were treated with vehicle or paclitaxel at the stated concentrations for 24 hours prior to analysis. (cyprotex.com)
Professor Summers is internationally recognised for his research excellence, which focuses on two areas: metrologies for cell analysis (cytometry) and the development of nanoparticle-based diagnostics and therapeutics (nanomedicine). (swansea.ac.uk)
Cell cycle analysis or BrdU incorporation for 72 h showed no differences between RF EMF- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G(0)/G(1)-phase and a reduction in S-phase cells. (monocyte.eu)
Power spectral analysis identified CCR5 oscillation frequencies synchronized with reproductive cycles. (cdc.gov)
Multivariate analysis at baseline indicated that greater CD4+ T cell abundance was associated with better cognition (p = .017), while higher CSF HIV RNA was associated with increased neuronal damage (p = .014). (cdc.gov)

Cancer cells that are defective in p53 function are deficient in their ability to transcriptionally upregulate the CDK inhibitor p21 after genotoxic stress. (nature.com)
In this study, we evaluate the efficacy of ACY-1215, a histone deacetylase 6 inhibitor (HDAC6i), to attenuate MUM cell growth in vitro and in vivo, and elucidate the underlying molecular mechanisms. (biorxiv.org)
CDK4/6 inhibitor washout results in some cells entering S-phase. (bvsalud.org)
In this study we investigated the effects of the MEK inhibitor trametinib, the ERK inhibitor SCH772984 and the CDK4/6 inhibitor ribociclib in human neuroendocrine tumor cell lines BON1, QGP1 and NCI-H727 in vitro. (uni-muenchen.de)
Objectives: This study assessed the antitumoral activity of the MEK inhibitor trametinib (TMT212) and the ERK1/2 inhibitor SCH772984, alone and in combination with the CDK4/6 inhibitor ribociclib (LEE011) in human neuroendocrine tumor (NET) cell lines in vitro. (uni-muenchen.de)

The effects of silibinin on the migration and invasion of oral cancer cells were evaluated using transwell assays. (jcancer.org)

In Experiments VI and VII, the effect of different levels of dimethylsulphoxide (DMSO) at 0%, 0.5%, 1.0% and 2.5% were tested by flow cytometry (FACS). (druglib.com)
A population study of coal-burning fluorosis and in vitro experiments were performed in this study. (fluoridealert.org)
Methodology Malignant granulosa tumour cell lines (COV434 and KGN) were used for the experiments. (bmj.com)
Additionally, in-vitro experiments, indicated that OEO/thymol interacts with DNA via minor grooves confirmed by docking method. (irost.org)
The biological function of LIPH-4 was examined in cell and animal studies, applying CCK-8, EdU, colony formation and flow cytometry assays as well as xenograft model experiments. (researchsquare.com)

Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)
The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
Central venous catheter placement is required before the administration of hematopoietic cell transplantation. (medscape.com)

However, its role has not been evaluated in hypoxic choroidal endothelial cells (CECs), the precursor of VEGF-mediated choroidal angiogenesis. (molvis.org)
In this study, we investigated the role of SIRT1 in choroidal angiogenesis in vitro and analyzed the mechanism by which SIRT1 may regulate the initiation of neovascularization. (molvis.org)
This study observes a previously neglected pharmacological phenomenon and investigates its mechanism of that the continuous low-dose administration of some antineoplastic agents in certain dose ranges can promote tumorigenesis and tumor progression in vitro and in vivo , through stimulation of tumor cell functions directly as well as enhancement of tumor angiogenesis by BMDCs recruitment indirectly. (researchsquare.com)

Choroidal endothelial cells (RF/6A) were maintained in a semiconfluent state and hypoxia was induced by exposing the cells to cobalt chloride for 24 h. (molvis.org)
BNP treatment increased vascularisation and the number of endothelial cells in all areas of infarcted hearts. (elifesciences.org)
Our results demonstrated that these immature cells have a natural capacity to differentiate into endothelial cells in infarcted hearts. (elifesciences.org)
One complementary therapy could be 're-activating' vasculogenesis (i.e. the differentiation of precursor cells into mature endothelial cells), a mechanism that occurs in the heart during development but is quiescent in adult hearts. (elifesciences.org)

Esophageal squamous cell carcinoma (ESCC) represents a major malignancy with poor clinical outcomes. (researchsquare.com)
Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)
The risk is increased 1000-fold for squamous cell carcinoma, basal cell carcinoma, malignant melanoma, and fibrosarcoma and is increased 10-fold to 20-fold for other tumors. (medscape.com)

MCF-7 cells, which have only one wild-type BRCA1 allele, were transfected with pcDNA3-beta-HA-hsBRCA1 plasmids via liposomes to reconstitute BRCA1 overexpression human breast cancer cell line, and tumoral xenografts with BRCA1 overexpression were subsequently established in BALB/c nude mice. (nih.gov)

Because BRCA mutations are observed in fewer than 10% of cancer patients (cBioPortal: 6.7%) 11 , 12 , 13 the identification of additional genes that share synthetic lethal sensitivity relationships with mutated oncogenes or tumor suppressors would greatly enhance the implementation of tumor cell-specific synthetic lethal sensitivity to improve an anticancer therapeutic response. (nature.com)
It is well established that statins display potent anticancer activity in several types of proliferating tumor cells. (nih.gov)
Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. (irost.org)
We aimed to examine the molecular processes underlying the effects of silibinin in oral cancer in vitro and in vivo as well as its potential anticancer effects. (jcancer.org)
Introduction: Many anticancer drugs their activity base on stopping cell progression through the cell cycle. (unios.hr)

SL originally described a relationship between two genes, where alteration of either gene alone results in viable cells, but alteration (mutation, loss, or inhibition) of both genes simultaneously was lethal. (nature.com)
It is also responsible for inhibition of vascular endothelial growth factors receptors, plateletderived growth factor receptors, and stem cell factor receptor [ 8 , 9 ]. (hindawi.com)
Conclusions: MEK and ERK inhibition causes antiproliferative effects in human NET cell lines in vitro. (uni-muenchen.de)

Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)

The objective of the current study was to explore possible cellular responses to the methacrylate monomer 2-hydroxy-propylmethacrylate (HPMA) in vitro . (niom.no)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (nih.gov)
The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (nih.gov)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Other cellular components of the innate immune system include mononuclear phagocytes, dendritic cells, and NK cells. (medscape.com)

Then, the inhibitory activity of lovastatin on cancer cells and tumoral xenografts, and the underlying mechanism involving in cell-cycle regulatory proteins were analyzed. (nih.gov)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

The proliferative ability of MCF-7 cells treated with lovastatin was reduced compared to normal, and further decreased in the presence of excess BRCA1, detected by methyl thiazolyl tetrazolium and flow cytometry techniques in vitro or by 5-bromodeoxyuridine incorporation in vivo. (nih.gov)

The Flow Cytometry Core Facility offers a range of flow cytometric techniques. (embl.org)
Developing of novel flow cytometric techniques to meet EMBL's diverse scientific needs. (embl.org)

LRRC15 was expressed on stromal fibroblasts in many solid tumors (e.g., breast, head and neck, lung, pancreatic) as well as directly on a subset of cancer cells of mesenchymal origin (e.g., sarcoma, melanoma, glioblastoma). (aacrjournals.org)

AHCC mechanism of action was evaluated with immunoblotting and flow cytometry studies. (researchgate.net)
We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
In this paper, the mechanism of MicroRNA-16-5p in the occurrence and development of glioma was studied by the cell experiment in vitro. (ijpsonline.com)
Because of breakthroughs such as the "switching" mechanism of the p53 tumor suppressor gene, as well as many technical innovations, the number of investigators of cell death has rapidly expanded over the last few years. (nhbs.com)
BACKGROUND: The menstrual cycle influences HIV infection-risk in women, although the timing and underlying mechanism are unclear. (cdc.gov)

Next, we investigated the molecular processes underlying both in vitro and in vivo outcomes of silibinin treatment on oral cancer. (jcancer.org)
Extensive cross talks occur between the fetal and maternal compartments around the time of implantation which are reflected by morphologic, biochemical and molecular changes in the endometrial cells and the differentiating trophoblast cells. (brjnmims.org)
American Journal of Respiratory Cell and Molecular Biology. (lu.se)

Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro. (swansea.ac.uk)

Flow cytometry is also used to detect cancer & immunodeficiency disorders, and in hematology, it is used for organ transplantation studies and in other applications. (grandviewresearch.com)
After UMSC transplantation (UMSCT), the frequencies of Tregs and CD4 + memory T cells were significantly increased, and the frequencies of T helper (Th) 17 and CD4 + naive T cells were significantly decreased in peripheral blood (PB) of psoriasis patients. (nature.com)

This study hypothesized that coating type, incineration status, and time-dependent effects of ONC exposure would impact bronchial epithelial cell monolayer integrity, a key target following inhalation exposure. (cdc.gov)
Conversely, incinerated nanoclay byproducts caused decreased monolayer integrity, increased cell necrosis, and little evidence for reestablishment of the epithelial monolayer. (cdc.gov)

This technology is used in pharmaceutical research for drug discovery, stem cell research, and in vitro toxicity testing studies. (grandviewresearch.com)

Different cell cultures were exposed to different concentrations of HPMA (0-8 mM) for 24 hours. (niom.no)
I. Studies of MCa-11 cells grown as tumours in vivo and as exponential cultures in vitro. (johnshopkins.edu)

Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
We interestingly found that our 3D+OP9 system enriched a higher percentage of CD201 + c-kit + cells that showed more similar HSC-like features such as transcriptome level and CFU formation ability than CD201 - c-kit + cells, which have not been reported in the field of mouse PSCs hematopoietic differentiation. (biomedcentral.com)

In fact, only using the OP9 co-culture system combined with hematopoietic related cytokines, we hardly obtained the in vivo functional reconstitution hematopoietic cells from PSCs [ 9 ]. (biomedcentral.com)

Peripheral blood mononuclear cell (PBMC) samples from healthy women (n=10) were prospectively collected over the course of a menstrual cycle to profile T cell populations. (cdc.gov)
Immune properties from PBMC and vaginal lavage samples were measured by flow cytometry. (cdc.gov)

This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)

Western blotting revealed that silibinin downregulated SOD1 and SOD2 and triggered the JNK/c-Jun pathway in oral cancer cells. (jcancer.org)

In addition, increasing incidence of Cancer and HIV/AIDS, usage of flow cytometry in clinical trials, advent of application-specific flow cytometry products are further expected to provide growth platform to this market. (grandviewresearch.com)
The techniques described facilitate both understanding and regulation of cell death, promising to have immense value in the clinical treatment of cancer. (nhbs.com)
The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)

Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)
2 Psoriasis is a disease with innate and adaptive immune system disorder and dendritic cells, neutrophils, keratinocytes and T cells play major roles in the pathogenesis. (nature.com)
The proinflammatory T cells secrete inflammatory cytokines, including interferon (IFN)-γ and IL-17, which affect keratinocytes and other leukocytes that forms an amplified immune response. (nature.com)
Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
The generation of receptors specific for antigens is a unique and complex process that generates 10 12 specific receptors for each cell type of the adaptive immune system, including T and B cells. (medscape.com)
The adaptive immune system consists of 2 types of lymphocytes: T cells (70-75% of the adaptive immune force) and B cells (10-20% of the adaptive immune force). (medscape.com)
Here we investigated the contribution of the menstrual cycle to HIV susceptibility through evaluating immune behavior with infection-risk over time. (cdc.gov)
INTERPRETATION: These data demonstrate that periodic shifts in the immune landscape under menstrual cycle regulation drives bystander CCR5+ CD4 T cell recruitment and HIV susceptibility in the female reproductive tract. (cdc.gov)
We examined the relationship between CSF immune cells and neurocognition and neuronal damage in HIV+ individuals before and after initiating antiretroviral therapy. (cdc.gov)

Mesenchymal stem cells (MSCs) have immunomodulatory properties. (nature.com)
Although hematopoietic stem and progenitor cells (HSPCs) enable autologous treatment of blood disorders, de novo generation of hematopoietic cells from pluripotent stem cells still compromises the unlimited high yield and rich hematopoiesis engraftment potential [ 2 ]. (biomedcentral.com)
The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)

Different concentrations of the Bushen Yizhi Formula and amyloid-beta peptide (Aβ) were used to treat rat pheochromocytoma cells (P12) and human neuroblastoma cells (SH-SY5Y). (techscience.com)
Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2a K Isotype Control Super Bright 702 (Product # 67-4724-82) (left) or Anti-Human CD3 Super Bright 702 (right). (thermofisher.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
This investigation aims to analyse the in vitro effects of cisplatin and sunitinib malate in isolation and in combination, on one human nonmuscle invasive urinary bladder-cancer cell line (5637) and on two human muscle-invasive urinary bladder-cancer cell lines (T24 and HT1376). (hindawi.com)
Oocysts from 21 human stool specimens were separated from other stool components using discontinuous density gradient centrifugation and purified further by flow cytometry. (cdc.gov)

Via flow cytometry in both cell lines a clear transformation of cell cycle induced by treatment with the drugs could be demonstrated, but these effects seem to be rather a single effect of flavopiridol. (uni-marburg.de)
Because most tumors are deficient in one or more aspects of the function of the p53 tumor suppressor, either as a consequence of mutations within p53, or impairment of upstream and downstream modulators of p53 activity 19 , targeting MK2 has the potential to selectively enhance tumor cell killing without increasing the genotoxic effects of chemotherapy on normal p53-wild type tissues. (nature.com)
Experiment I focused on the effects of cycling, serum-starved, and fully confluent stages of Goral cells. (druglib.com)
However, the conventional chemotherapy with antineoplastic agents at the maximum tolerated dose (MTD) is typically administrated in short cycles, obligatorily separated by long treatment breaks (e.g., 3 weeks), and unfortunately accompanied by serious toxic side effects. (researchsquare.com)
Highthroughput in vitro screening strategies including high content imaging, electric cell impedance sensing, and flow cytometry were employed to evaluate a set of pre- and post-incinerated ONCs for acute effects and fate of the monolayer post-exposure. (cdc.gov)

Cell cycle distribution was determined using flow cytometry and a fluorescent ubiquitin-based cell cycle indicator (FUCCI) system combined with histone H3 phosphorylation at Ser10 (pS10 H3) detection. (bvsalud.org)

The purpose of this study was to investigate whether breast cancer susceptibility gene 1 (BRCA1) overexpression sensitizes mammary cancer cells to statins. (nih.gov)
Investigate genotoxicity using a flow cytometry based micronucleus test in TK6 cells. (cyprotex.com)

Summary of data for genotoxic and non-genotoxic reference compounds in the in vitro micronucleus test using TK6 cells and flow cytometry. (cyprotex.com)

ELAM-1) both in resting and in ET-B -activated ECV304 cells in a dose-dependent manner [20]. (cancercurehere.com)

in BG1-cells combining both drugs led to a rise of necrosis and a reduction of vital cells. (uni-marburg.de)

Cell death pattern was analyzed by fluorescence microscopy of cells stained with propidium iodide (PI) and Hoechst 33342. (niom.no)
Flow cytometry quantifies marker intensity (Hoechst, CFDA-SE, EMA) and object size to detect micronuclei. (cyprotex.com)

CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)

A complete blood cell count (CBC) with differential should be obtained, including examination of the peripheral blood smear if the differential is abnormal. (medscape.com)

The France flow cytometry market is expected to register growth during the forecast period owing to, technical development within the field of flow cytometry. (grandviewresearch.com)
In our study, we identified that the drug ACY-1215 prevents the growth of UM cells from the liver. (biorxiv.org)

Many studies have reported their regulatory roles in immunity through cytokines and cell signaling. (cancerindex.org)
Single-cell sorting for single cell quantitative genome, transcriptome, CRISPR/Cas9 genome editing and in vitro studies. (embl.org)
In some other studies, substantial pre-existing T-cell reactivity to SARS-CoV-2 was detected in many people from diverse geographical locations without a history of exposure. (bvsalud.org)

The present study was undertaken to examine cell cycle characteristics of endangered Goral (CITES Appendix I) adult skin fibroblasts. (druglib.com)
The study was performed on the 5637, T24, and HT1376 urinary bladder-cancer cell lines. (hindawi.com)
Especially, this study revealed for the first time that pomalidomide enhances p53 on pancreatic cancer cells. (oncotarget.com)
In the current study, Chlamydomonas reinhardtii (Cr) cells were attributed to sodium nitrate stress in concentrations such as 5 mM, 10 mM, 20 mM, 30 mM, and a control to determine the productivity and bioactivity of SPs. (brjnmims.org)
In the tradition of this long-standing series, this volume's user-friendly recipes and more than 100 tables and figures make the study of cell death accessible across life science disciplines. (nhbs.com)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)
Infants suffering of cell death occurring as a series of changes from chronic illnesses, chromosomal abnor- in dying cells under several physiological malities and those on medication prior to conditions [ 6,7 ] and has been implicated enrolment were excluded from the study. (who.int)

B.A. Osborne, S.W. Smith, Z.-G. Liu, K.A. McLaughlin, and L.M. Schwartz, Transient Transfection Assays to Examine the Requirement of Putative Cell Death Genes. (nhbs.com)

11 The regulatory effect of MSCs on T cells has also been demonstrated in mice models of autoimmune disease such as rheumatoid arthritis and sjogren syndrome (SS). (nature.com)

Anatomy38

Organisms10

Diseases7

Chemicals and Drugs92

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes80

Disciplines and Occupations2

Information Science3

Health Care4

Proliferation28

Apoptosis25

Assay6

Viability5

Apoptotic7

Arrest15

Cytotoxicity5

Methods6

Protein10

Cyclin-Dependent Kinase1

Findings6

Analysis25

Inhibitor5

Transwell1

Experiments5

Hematopoietic cell3

Angiogenesis3

Endothelial Cells4

Squamous cell carc3

Nude1

Anticancer5

Inhibition3

Tumor cells1

Cellular5

Proteins2

Tetrazolium1

Cytometric2

Lung1

Mechanism5

Molecular3

Lymphoblastoid1

Transplantation2

Epithelial2

Toxicity1

Cultures2

Differentiation3

Vivo functional1

PBMC2

Therapeutics1

Pathway1

Clinical3

Immune9

Stem4

Human5

Effects5

Fluorescent1

Investigate2

Genotoxic1

Dose dependent manner1

Necrosis1

Hoechst2

Significantly1

Peripheral1

Growth2

Studies3

Study7

Examine1

Mice1