Vitro endothelial progenitor and hematopoietic cells. Medical search. Frequent questions

Induces upregulation of the pluripotency network genes (Klf4, Klf2, Nanog, Oct4, Myc, Sox2) and epigenetic-associated proteins (Dnmt3a, Mbd3) in mouse ES cells, and inhibits retinoic acid-induced differentiation (Adamo et al. (stemcell.com)
Promotes differentiation and mineralization of MC3T3-E1 osteoblastic cells (Kanazawa et al. (stemcell.com)
Inhibits proliferation and promotes osteogenic differentiation, while inhibiting adipogenic differentiation, of human amnion-derived mesenchymal stem cells (MSCs) and rabbit bone marrow-derived MSCs (Wu et al. (stemcell.com)
Decreased proliferation and Nanog expression in mouse embryonic stem (ES) cells, with increased erythroid differentiation (Chae et al. (stemcell.com)
To our surprise, we found that in the absence of any manipulation, endothelial cells could direct differentiation and support the expansion of CD34+ cells (progenitor blood cells) to a very specific blood cell type, named macrophages. (ca.gov)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
After the in vitro differentiation of these embryonic stem cells to embryoid bodies, developing mesodermal progenitors could be separated from those with neuroectoderm potential based on GFP expression. (elsevierpure.com)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)
Embryonic blood cell development occurs via well-defined developmental stages which are recapitulated in vitro by differentiation of embryonic stem cells. (nih.gov)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
The BMP, Wnt and Nodal signaling requirements for the differentiation of specific mesoderm subtypes in mammals have been mapped in detail, but how FGF shapes mesodermal cell type diversity is not precisely known. (biologists.com)
Although the main cell-cell communication systems that drive and coordinate differentiation in cell populations have been identified, how these systems interact in signaling networks is still not well understood. (biologists.com)
that critically influences cell proliferation, differentiation and apoptosis ( Miele and Osborne, 1999 ). (elifesciences.org)
US8557580 on October 15, 2013 entitled "Methods and Compositions for the Differentiation of Stem Cells" by USPTO. (pullpreview.com)
and b) differentiating the dispersed cells in a differentiation culture medium comprising at least one recombinant growth factor selected from the group consisting of BMP-4, VEGF, and bFGF, under a hypoxic atmosphere having less than or equal to 5.5% oxygen for a period of time to provide the CD34+ progenitor cells. (pullpreview.com)
The Hirschi Lab is broadly interested in endothelial cell differentiation, proliferation, specification and function during development. (hirschilabuva.com)
The lab also uses human stem cell culture models to study the regulation of human endothelial cell differentiation and specification, and to study the functional roles of endothelial cells in regulating tissue-resident stem cells. (hirschilabuva.com)
Regulation of endothelial cell differentiation and specification. (hirschilabuva.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
Role of extracellular RNA-carrying vesicles in cell differentiation and reprogramming. (unicyte.ch)
In particular, stem cells are highly sensitive to extracellular signals that play a critical role in mainten- ance of stem cell characteristics, differentiation, and interplay with somatic cells. (unicyte.ch)
Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)

Following early observations that MSCs inhibit T-cell proliferation, 9 MSCs were found to interact with the majority of innate and adaptive immune cells. (bmj.com)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
[8] Binding of TSP-1 to CD47 influences several fundamental cellular functions including cell migration and adhesion, cell proliferation or apoptosis, and plays a role in the regulation of angiogenesis and inflammation. (wikidoc.org)
The role of CD47 in promoting cell proliferation is heavily dependent on cell type as both activation and loss of CD47 can result in enhanced proliferation. (wikidoc.org)
Activation of CD47 with TSP-1 increases proliferation of human U87 and U373 astrocytoma cells but not normal astrocytes . (wikidoc.org)
Additionally, CD47 blocking antibodies inhibit proliferation of unstimulated astrocytoma cells but not normal astrocytes. (wikidoc.org)
Though the exact mechanism is unclear, it is likely that CD47 promotes proliferation via the PI3K/Akt pathway in cancerous cells but not normal cells. (wikidoc.org)
Loss of CD47 allows sustained proliferation of primary murine endothelial cells and enables these cells to spontaneously reprogram to form multipotent embryoid body-like clusters. (wikidoc.org)
Activation of CD47 with TSP-1 in wild-type cells inhibits proliferation and reduces expression of stem cell transcription factors. (wikidoc.org)
The aim of this thesis is to study proliferation, differentition and location of endogenous progenitors and their roles in PTS. (edu.au)
An in vitro study on the effect of hyperglycemia on the proliferation and viability of the cultured EPCs was also performed. (biomedcentral.com)
When glucose was supplemented in the culture in vitro , there was a negative effect on the proliferation and viability of EPCs, in a dose-dependent manner, whereas the enhancement of apoptosis was observed. (biomedcentral.com)
In vitro hyperglycemic effect using various glucose concentrations on the viability, proliferation and apoptosis of cultured EPCs from both diabetic patients and healthy controls were also performed. (biomedcentral.com)
In addition, endothelial proliferation and growth of capillary blood vessels in the bone marrow are observed and may be a result of TGF-β and bFGF production. (medscape.com)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
iii) absence of several haematopoietic and endothelial markers (ie, CD45, CD34, CD11b or CD14, CD79 or CD19 and HLA-DR in human MSCs). (bmj.com)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)

Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)

Unfortunately, there are no unique markers for endothelial progenitors that are not shared with other endothelial or hematopoietic cells, which has contributed to the historical controversy surrounding the field. (wikipedia.org)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
1 Unique MSC-specific markers have not yet been identified, and MSCs constitute a heterogeneous cell population, including both multipotent (stem) cells and progenitor cells and might even contain pluripotent cell fractions. (bmj.com)
Flow cytometry analysis showed a significantly higher number of hematopoietic stem/endothelial progenitor cell markers in concentrated BMMCs when compared with bone marrow aspirate indicating an enrichment of these cell types. (bioskinrevive.com)
Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
VSELs have already been described as uncommon little pluripotent stem cells within murine and individual BM [7 8 Kaempferol-3-O-glucorhamnoside They're lineage- and Compact disc45-harmful express stem-cell markers and present rise to cells matching to all or any three germ levels in vitro [9]. (cancer-pictures.org)
(B) Subset of cell surface markers used in the characterization of this developmental process. (biomedcentral.com)
Expression of several stem cell markers , including c-Myc , is elevated in CD47-null endothelial cells and a human T cell line lacking CD47. (wikidoc.org)
HLSCs do not express hematopoietic stem cell markers, whereas they express several mesenchymal markers and display multiple differentiating abilities ( 22 ). (spandidos-publications.com)
Proliferating cells were marked by either exogenous mitotic marker bromodeoxyuridine or endogenous mitotic marker Ki67.lmmunofluorescence techniques targeting mitotic markers were used to trace the proliferating cells. (edu.au)

Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)
E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells(HPCs) into bone marrow(BM). (innov-research.com)

Our investigation indicates that most of these studies examined endothelial progenitor cells, hematopoietic stem cells, mesenchymal stem cells, adipose-derived stem cells, and a few others were about less-discussed types of stem cells such as cardiac stem cells, myeloblasts, and induced pluripotent stem cells. (magiran.com)
Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
CDI (Cellular Dynamics International), a leader in developing induced pluripotent stem cell technologies for in vitro drug development, in vivo cellular therapeutic research and stem cell banking was granted patent no. (pullpreview.com)
However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)

Mesenchymal stromal cells (MSCs) are cells of non-haematopoietic origin, with the capacity to differentiate into multiple lineages of the mesenchyme, that is, chondrocytes, osteoblasts and adipocytes. (bmj.com)
The hematopoietic and endothelial lineages derive from mesoderm and are thought to develop through the maturation of a common progenitor, the hemangioblast. (elsevierpure.com)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)

While some other authors have contested these, and maintained that they are true EPCs, many investigators have begun to term these cells colony forming unit-Hill cells (CFU-Hill) or circulating angiogenic cells (CAC) instead (depending on the method of isolation), highlighting their role as hematopoietic myeloid cells involved in promoting new vessel growth. (wikipedia.org)
By method of isolation and cell function, three main populations of putative adult EPCs have been described. (wikipedia.org)
Certain developmental cells may be similar to or the same as other endothelial progenitors, though not typically referred to as EPCs. (wikipedia.org)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Endothelial progenitor cells (EPCs) play an important role in vascular repair and a decrease in the number of EPCs is observed in type 2 diabetes. (biomedcentral.com)
One particular element that is beginning to garner interest involves the systemic mobilization of endothelial progenitor cells (EPCs). (biomedcentral.com)

Although bone marrow-derived cells do appear to localize to injured vessels and promote an angiogenic switch, other studies have suggested these cells do not contribute directly to the functional endothelium, instead acting via paracrine methods to provide support for the resident endothelial cells. (wikipedia.org)
Ablation of the endothelial progenitor cells in the bone marrow lead to a significant decrease in tumour growth and vasculature development. (wikipedia.org)
Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
2 MSCs were first described in the bone marrow where they constitute a small fraction of cells (0.001%-0.01%) that closely interact with haematopoietic cells to support haematopoiesis and skeletal homeostasis. (bmj.com)
Introduction Stem cell therapy with bone marrow-derived mononuclear cells (BMMCs) is an option for improving joint function in osteonecrosis of the femoral head (ONFH). (bioskinrevive.com)
Cytokines and growth factors (interleukin-8 transforming growth factor-beta stromal cell-derived factor-1alpha and vascular endothelial growth factor) that mediate endogenous bone regeneration were also produced by expanded MSCs from SCD patients. (bioskinrevive.com)
The authors of this paper have published one, two, three and five-year follow-up from a study assessing the safety and efficacy of bone marrow concentrate [BMC] cells as an alternative to surgery for discogenic back pain at one or two levels [9,10,11,38]. (medicalpressopenaccess.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
Introduction Several adult non-hematopoietic Kaempferol-3-O-glucorhamnoside stem cells have already been identified within the bone tissue marrow (BM) up to now. (cancer-pictures.org)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Cell types that are included in hematopoietic cells are erythroid cells (RBC), lymphocytes (WBC) and myelocytes (Bone marrow cells). (pullpreview.com)

Mesenchymal stromal cells: a novel therapy for the treatment of chronic obstructive pulmonary disease? (bmj.com)
In vitro cell culture and animal studies have demonstrated that mesenchymal stromal cells (MSCs) have the capacity to modify immune responses and to enhance tissue repair. (bmj.com)
There have been numerous studies utilizing mesenchymal stem cells to enhance tissue repair and decrease inflammatory damage in both in vitro lab studies and in vivo clinical models. (medicalpressopenaccess.com)
It is known that BMC contains mesenchymal stem cells as well as a number of other cell types including but not limited to: hematopoietic stem cells, endothelial progenitor cells, and platelets. (medicalpressopenaccess.com)
Studies have shown both the mesenchymal stem cell population and other nucleated cell types have healing properties and may contribute synergistically to the healing seen in studies on the spine [19,20,21,22]. (medicalpressopenaccess.com)
During embryogenesis endothelial cells exhibit substantial plasticity that contribute to cardiac development by undergoing endothelial-to-mesenchymal transition (EndoMT). (surrey.ac.uk)
CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. (thermofisher.com)
These growth factors are mitogens for cells of mesenchymal origin. (thermofisher.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

Developmentally, the endothelium arises in close contact with the hematopoietic system. (wikipedia.org)
Angioblasts are believed to be a form of early progenitor or stem cell which gives rise to the endothelium alone. (wikipedia.org)
In the second year of this proposal, we have made significant progress and identified critical factors that are responsible for the generation of hematopoietic stem cells from the endothelium (inner layer of blood vessels). (ca.gov)
Although the hemogenic capacity of the endothelium is, under normal conditions, restricted development we have been able to "reprogram" this ability in endothelial cells. (ca.gov)
Using this strategy we found key transcription factors in hemogenic endothelium not present in other (non-hemogenic) endothelial cells. (ca.gov)
Here we show that FOS and JUN together bind to and activate a core set of vascular genes in the hemogenic endothelium and that upon global inhibition of AP-1 by expression of a dominant negative FOS peptide the balance between endothelial and hematopoietic fate is shifted towards blood. (nih.gov)
Expression system for standard: E-selectin, also called endothelial-leukocyte adhesion molecule-1(ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. (innov-research.com)
by enhancement of the endothelium derived nitric oxide (NO) production[8, 9], the expression of vascular endothelial growth factor (VEGF) and VEGF-receptor 2 and activation of endogenous fibroblasticgrowth factor -2 [10, 11]. (alk-inhibitors.com)

whereas, vascular endothelial growth factor‑A levels were positively correlated with Angptl7 levels. (spandidos-publications.com)

Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
Peripheral smear shows teardrop red blood cells (RBCs) and a leukoerythroblastic picture with nucleated RBC precursors and immature myeloid cells. (medscape.com)

This information will be used to recapitulate similar conditions in vitro and induce the growth of hematopoietic stem cells outside the body from adult endothelial cells. (ca.gov)
The first step was to test whether human endothelial cells could offer a supportive niche for the growth of hematopoietic cells. (ca.gov)

These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)

This capacity, called "hemogenic" (giving rise to blood) can be extremely advantageous in pathological situations when generation of new blood cells are needed, such as during leukemia or in organ-transplantation. (ca.gov)
For this, we first investigated the genes that responsible for this hemogenic activity during development using mouse models and tissue culture cells. (ca.gov)
Ongoing research is focused on understanding mechanisms of endothelial cell specification towards hemogenic, arterial, venous and lymphatic fates. (hirschilabuva.com)
Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification. (hirschilabuva.com)
Research in the Hirschi Lab aims to uncover the developmental mechanisms of hemogenic endothelial cell specification and endothelial-to-hematopoietic transition, and apply insights gained to the generation of human HSPCs for clinical therapies. (hirschilabuva.com)

Endothelial progenitor cells are likely important in tumour growth and are thought to be critical for metastasis and the angiogenesis. (wikipedia.org)
MicroRNA-126 (miR-126) is endothelial cell (EC) specific and plays a key role in regulating EC function, controlling angiogenesis, and maintaining vascular integrity. (justia.com)
This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
CD47 is a high affinity receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis, and in this later capacity the TSP1-CD47 interaction inhibits nitric oxide signaling at multiple levels in vascular cells. (wikidoc.org)
Several recent experiments have suggested that the growth of some types of tumors is not only dependent on angiogenesis (i.e., mature endothelial-cell dependent generation of new blood vessels) but also is associated with vasculogenesis, which means endothelial progenitor cell (EPC) dependent generation of new blood vessels [2]. (alk-inhibitors.com)

the CFU-Hill population is an early outgrowth, formed by plating peripheral blood mononuclear cells on fibronectin-coated dishes, allowing adhesion and depleting non-adherent cells, and isolating discrete colonies. (wikipedia.org)
Quickly the BM- or HAR-NDS-derived mononuclear cells had been resuspended in Kaempferol-3-O-glucorhamnoside cell-sort moderate (CSM) made up of 1% heat-inactivated. (cancer-pictures.org)
Jurkat cells and peripheral blood mononuclear cells ( PBMC ) incubated with the monoclonal antibody Ad22 results in apoptosis within 3 hours. (wikidoc.org)
Notably, autologous cord blood mononuclear cells (ACBMNCs) can substantially prevent severe BPD and decrease the inflammatory response in surviving very preterm neonates. (spandidos-publications.com)
Our previous study demonstrated that autologous cord blood mononuclear cells (ACBMNCs), which are rich in stem cells, could substantially prevent moderate or severe BPD in surviving very preterm neonates, and that the immunomodulatory effect of MNCs contributed in mitigating the severity of BPD ( 13 ). (spandidos-publications.com)

Hence the discovery is reported simply by us of potential adult stem cells which may be involved with tissue regeneration. (cancer-pictures.org)
Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
Vesicles derived from adult human liver stem-like cells (HLSCs) have also been shown to contain ribonucleoproteins and different RNA species involved in hepatic regeneration ( 20 ). (spandidos-publications.com)
Hematopoietic stem and progenitor cells (HSPCs) that are essential for establishment and maintenance of the adult blood system are generated during embryonic definitive hematopoiesis. (hirschilabuva.com)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)

Endothelial colony forming cells represent a distinct population that has been found to have the potential to differentiate and promote vessel repair. (wikipedia.org)
During the development of multicellular organisms, precursor cells for specialized organs need to differentiate in reproducible proportions and in spatially coherent domains. (biologists.com)
had higher sphere-forming efficiency and proliferative potential than VSELs and they were present to differentiate into neuronal cells in vitro. (cancer-pictures.org)
10] reported they differentiate vivo into lung epithelial cells in. (cancer-pictures.org)
We also demonstrate that this NDSCs have the potential to differentiate into neuronal cells and to repair ischemic injury in the brain. (cancer-pictures.org)
The patent was about the method by which stem cells are induced to differentiate into different types of endothelial (blood vessel) and hematopoietic cells (blood). (pullpreview.com)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
Endogenous stem cells were found to differentiate into astrocytes. (edu.au)
Endogenous progenitors differentiate into astrocytes but not oligodendrocytes or neurons within 8 weeks. (edu.au)
Endogenous progenitors in the PTS model differentiate into astrocytes, which help to form the glial scar lining the syrinx. (edu.au)

This was a very exciting result as indicated that we are in the right track and identified factors that can reprogram blood vessels to bud blood stem cells. (ca.gov)
The horizontal transfer of extracellular RNAs carried by EVs has been shown to be able to reprogram hematopoietic progenitors ( 11 ) and to activate endothelial cells ( 12 ). (spandidos-publications.com)

More recently, mesoangioblasts have been theorized as a cell giving rise to multiple mesodermal tissues. (wikipedia.org)
To investigate the developmental processes that regulate mesoderm induction and specification to the hemangioblast, we generated an embryonic stem cell line with the green fluorescent protein (GFP) targeted to the mesodermal gene, brachyury. (elsevierpure.com)
Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)

In brief, my research team uses cutting edge imaging, clonal tracking (cellular barcoding), genomics technologies, and functional assays (in vivo and in vitro) to tackle long-standing questions about the biology of blood production. (edu.au)
For in vivo and in vitro studies, we employ next-generation sequencing and bioinformatic approaches to investigate underlying mechanisms. (hirschilabuva.com)

CD47 interacts with signal-regulatory protein alpha (SIRPα), an inhibitory transmembrane receptor present on myeloid cells . (wikidoc.org)

With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)

In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)
The intravascular HAR-NDS might serve as a route that delivers these stem cells with their target tissues. (cancer-pictures.org)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Vascular perfusion is required for all tissues in the body to provide nutrients and oxygen, remove waste products, maintain fluid homeostasis, and traffic immune cells. (hirschilabuva.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)

Co-expression of GFP with the receptor tyrosine kinase Flk1 revealed the emergence of three distinct cell populations, GFP - Flk1 - , GFP + Flk1 - and GFP + Flk1 + cells, which represent a developmental progression ranging from pre-mesoderm to prehemangioblast mesoderm to the hemangioblast. (elsevierpure.com)
The hematopoietic developmental process has been studied in detail since the early 1900s. (biomedcentral.com)
We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)

Aliotta et al ( 15 ) demonstrated that lung-derived vesicles carried RNA to marrow cells and altered the phenotype of these cells both genetically and functionally. (spandidos-publications.com)
Immunofluorescent double staining techniques were used to phenotype the proliferating cells. (edu.au)
Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)

Hemangioblasts (or their in vitro counterpart, blast-colony forming cells) are cells believed to give rise to both the endothelial and hematopoietic systems during early development. (wikipedia.org)

cells which could give rise to functional vasculature in adults. (wikipedia.org)
5 Functional in vitro assays indicate different physiological roles of MSCs related to their heterogeneity and tissue location of origin. (bmj.com)
We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)
What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
In the present review we focus on the extracellular vesicle-induced epigenetic changes in recipient cells that may lead to phenotypic and functional modifications. (unicyte.ch)
Information exchange between cells coordinates devel- opment and functional interplay in complex organisms. (unicyte.ch)

However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
Further study will be required in the future to better understand succinate biology in blood cells. (haematologica.org)
Investigation into the reaction of endogenous progenitors in PTS may extend our knowledge about stem cell biology and help to develop a new treatment option for PTS. (edu.au)
The relevance of these phenomena in stem cell biology and tissue repair is discussed. (unicyte.ch)

The CD47/SIRPα interaction leads to bidirectional signaling, resulting in different cell-to-cell responses including inhibition of phagocytosis , stimulation of cell-cell fusion, and T-cell activation . (wikidoc.org)

While there is conflicting evidence with regards to the delta of endothelial progenitor cell mobilization and burn severity, it is clear that they play an important role in wound healing. (biomedcentral.com)

Endothelial progenitor cell (or EPC) is a term that has been applied to multiple different cell types that play roles in the regeneration of the endothelial lining of blood vessels. (wikipedia.org)
During this year, we have demonstrated that hematopoietic stem cells are originated from the cells that line the inside of blood vessels, named endothelial cells. (ca.gov)
The objective of this proposal was to identify factors that allow blood vessels to generate hematopoietic stem cells early in the embryonic stage. (ca.gov)
Endothelial cells are used in the research of vascular diseases that includes atherosclerosis (accumulation of plaques in blood vessels that damages tissue and lead to cell damage), inflammatory response to injury, healing of tissue and formation of blood vessels. (pullpreview.com)
In this year, CDI was issued this as fifth patent and on this achievement Bob Palay, Chairman and CEO of CDI stated that the patent covered a method that would be reliable and scalable to create consistent supply of blood vessels and the cells for the same from iPS would be of high quality and purity. (pullpreview.com)

The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro . (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)

It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. (ixcellsbiotech.com)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

These original, early outgrowth, CFU-Hill or CACs are also shown to express CD14, a lipopolysaccharide receptor expressed by monocytes but not endothelial cells. (wikipedia.org)
A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. (innov-research.com)
PDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. (thermofisher.com)
Recent evidence obtained in mouse models shows its essential role regulating blood cell function through various mechanisms that include pseudohypoxia responses by hypoxia-inducible factor-1α activation, post-translational modifications like succinylation, and communication mediated by succinate receptor 1. (haematologica.org)
GPR91 is a G protein-coupled cell surface receptor for extracellular succinate (Sucnr1). (haematologica.org)
Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)
The ACK2 monoclonal antibody specifically binds to CD117, which is also known as Proto-oncogene c-Kit (c-kit), Stem Cell Factor Receptor (SCFR), and Mast/stem cell growth factor receptor. (bdbiosciences.com)
CD117 (c-kit) serves as a receptor for c-Kit ligand (aka, stem cell factor or SCF). (bdbiosciences.com)

Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Their results indicated that yolk sac progenitors before the onset of circulation did not have LT reconstitution potential while progenitors isolated from the AGM did, which indicates that primitive and definitive hematopoiesis may occur at distinct anatomical locations [ 10 ]. (biomedcentral.com)

A similar method is to culture the peripheral blood mononuclear fraction in supplemented endothelial growth medium, removing the non-adherent cells, and isolating the remaining. (wikipedia.org)
ECFCs are isolated by plating peripheral blood mononuclear fraction on collagen-coated plates, removing non-adherent cells, and culturing for weeks until the emergence of colonies with a distinctive cobblestone morphology. (wikipedia.org)
These results suggest that the exposure to environmental pollutants in utero affects genetic constitution of fetus and increases MN values in their body cells, which is detectable right after birth in peripheral blood lymphocytes. (go.jp)

Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. (ixcellsbiotech.com)

There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)

With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)

Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. (elifesciences.org)
Endothelial cell specialization is associated with suppression of cell growth, but how signaling pathways that control endothelial cell cycle and fate are coordinated was not known. (hirschilabuva.com)
In subsequent studies using the Fluorescent Ubiquitination Cell Cycle Indicator (FUCCI) reporter, we found that endothelial cell cycle state determines propensity for arterial-venous fate. (hirschilabuva.com)
Endothelial Cell Cycle State Determines Propensity for Arterial-Venous Fate. (hirschilabuva.com)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)

Molecular genetic analysis of early outgrowth putative EPC populations suggests they do indeed have monocyte-like expression patterns, and support the existence of a separate population of progenitors, the late outgrowth, or endothelial colony forming cell (ECFC). (wikipedia.org)
Furthermore, early outgrowth cells maintain other monocyte functions such as high Dil-Ac-LDL and India ink uptake and low eNOS expression. (wikipedia.org)
Furthermore leptin increased the adhesion, transmigration, and incorporation of early outgrowth progenitor cells into experimental arterial lesions [15]. (alk-inhibitors.com)

ECFCs are now known to be tissue-resident progenitor cells in adults that maintain some vasculogenic ability. (wikipedia.org)
Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
He also talked about the company's commitment towards innovation and would work to create tissue cells that are critical to research that would have an impact on human health. (pullpreview.com)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
Endogenous stem cell therapy, aiming at replacing lost tissue and repairing damaged ones by endogenous progenitors, may offer hope. (edu.au)

To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
Several experimental strategies have been developed to derive iPSCs from differentiated somatic cells (summarized in Figure 1 ). (biomedcentral.com)

With this information at hand, we moved into human cells (in petri dishes). (ca.gov)
Finally, using human endothelial cells, we have been able to impose expression of these key transcription factors in endothelial cells. (ca.gov)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Since the main problem was the leakage of several tones of polychlorinated biphenyls (PCBs) as well as heavy metals Cr and Ni into the environment and groundwater, we decided to evaluate influence of the environmental contamination on eventual changes of genetic constitution of human body cells. (go.jp)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)
Our products are intended for in-vitro use only for manufacturing and research and are not suitable for human or animal use. (innov-research.com)
CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. (wikidoc.org)
The aim of the present study was to evaluate whether Alix is involved in the packaging of miRNAs within EVs released by human liver stem‑like cells (HLSCs). (spandidos-publications.com)
EVs were instrumental in transferring selected miRNAs from HLSCs to human endothelial cells absent in the latter cells. (spandidos-publications.com)
Angiopoietin‑like protein 7 (Angptl7) is one of the main paracrine cytokines in cord blood stem cells, and is capable of stimulating human hematopoietic stem and progenitor cell expansion. (spandidos-publications.com)
Angptl7 has been shown to be one of the most abundant paracrine cytokines secreted by stem cells, which is capable of stimulating human hematopoietic stem and progenitor cell expansion ( 15 , 16 ). (spandidos-publications.com)
In our previous study, it was revealed that Angptl7 was capable of stimulating human hematopoietic stem and progenitor cell expansion, and increasing the repopulation activities of human hematopoietic progenitors ( 15 , 16 ). (spandidos-publications.com)
Non-human primates (NHPs) can sustain viral replication in relevant cell types and develop a robust immune response, but they do not develop overt disease. (mdpi.com)

Antibodies and fluorescence-activated cell sorting evaluation VSELs and NDSCs had been isolated from a suspension system of total nucleated cells in the BM and HAR-NDS respectively by live sterile cell sorting. (cancer-pictures.org)

Stem cell senescence in the in vitro expansion stage is an essential concern in clinical procedures of cell therapy. (magiran.com)
Moreover, in vitro studies are the first step for further in vivo and clinical studies. (magiran.com)
In this review, we discuss the rationale for MSC-based cell therapy in COPD, the main findings from in vitro and in vivo preclinical COPD model studies, clinical trials in patients with COPD and directions for further research. (bmj.com)
Clinical and radiographic findings were assessed and data were completed by in vitro analysis. (bioskinrevive.com)
This research considered the remodelling aspects of these conditions using both in vitro and pre-clinical models. (surrey.ac.uk)
If these cells are available readily then improvements in clinical setting like cancer treatments, cell-based therapies, organ transplantation and autoimmune diseases could be achieved. (pullpreview.com)
Notably, stem cell-based paracrine cytokine treatment, with its anti-inflammatory and immunoregulatory ability ( 9 , 10 ) has been regarded as a promising therapy for BPD in preclinical models and clinical studies ( 10 - 12 ). (spandidos-publications.com)
This promising field may open new avenues to modulate inflammatory responses and to preserve blood cell homeostasis in the clinical setting. (haematologica.org)

Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)

Stromal cell feeder layers (e.g. (stemcell.com)

On the basis of the unlimited capacity to be propagated in vitro , iPSCs are good targets for genetic manipulation by gene therapy or gene correction by homologous recombination. (biomedcentral.com)
This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)

MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)

Notably, after the endothelial-to-hematopoietic transition TEAD4 binding is no longer persisting. (nih.gov)

Anatomy54

Organisms14

Diseases22

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes48

Disciplines and Occupations2

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care2

Differentiation26

Proliferation18

CD3410

Embryonic stem2

Markers13

Primitive hematopoietic2

Pluripotent9

Lineages3

EPCs8

Bone11

Mesenchymal9

Endothelium8

Vascular endoth1

Precursors4

Growth of hematopoietic2

Mature endothelial cells1

Hemogenic5

Angiogenesis5

Mononuclear cells5

Adult6

Differentiate10

Reprogram2

Mesodermal3

Vivo and in vitro2

Myeloid cells1

Multipotent1

Tissues7

Murine embryonic1

Developmental3

Phenotype5

Colony formi1

Functional6

Biology4

Inhibition1

Mobilization1

Blood vessels5

Angiogenic3

Mast cells2

Receptor8

Yolk sac2

Peripheral3

Erythroid1

Proteins3

Regeneration1

Fate7

Early outgrowth3

Tissue6

Derive2

Human14

Fluorescence-activated cell1

Clinical8

Putative1

Stromal cell1

Gene4

Adherent1

Transition1