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  • murine
  • Here, we generated stably transformed murine ES (embryonic stem) cells that express a constitutively active form of myocyte enhancer factor 2C (MEF2CA). (jneurosci.org)
  • Here, using a constitutively active form of MEF2C (MEF2CA), we report the production and characterization of neuronally restricted progenitors derived from non-oncogene-transformed, murine embryonic stem (ES) cells, and their subsequent successful transplantation into a disease model of focal cerebral ischemia. (jneurosci.org)
  • tissue
  • Basic science in the field of embryonic development, stem cell differentiation, and tissue engineering has offered important insights into key pathways and scaffolds that regulate hESC differentiation, which have produced advances in modeling gastrulation in culture and in the efficient induction of endoderm, mesoderm, ectoderm, and many of their downstream derivatives. (hindawi.com)
  • Tissue engineering is an emerging field of research aimed at regenerating functional tissues by combining cells with a supporting substrate. (hindawi.com)
  • Although we could induce some retinal cells and observed a limited recapitulation of the 3-dimensional structure of ocular tissue in vitro, the efficiency and total number of these cells in the cultures were very low and disadvantageous for further analysis. (arvojournals.org)
  • Anatomy: cell types and tissue structure. (elifesciences.org)
  • Projection of single-cell data onto tissue structures: image shows the mapping of individual cells onto locations in the marine annelid brain, based on the correspondence (color bar) between their single-cell expression profiles and independent FISH assays for a set of landmark transcripts. (elifesciences.org)
  • 2002, "Growth Inhibitors Promote Differentiation of Insulin-Producing Tissue from Embryonic Stem Cells," PNAS, vol. 99(25)16105-16110. (patentgenius.com)
  • One day before transfection, mouse D3 ES cells were trypsinized and plated in DMEM plus 20% FBS and 1000 U/ml LIF in six-well tissue culture plates coated with gelatin at a density resulting in ∼90% confluency within 1 d. (jneurosci.org)
  • 4 Its use can be limited, however, by a requirement for the eliminated gene product in normal animal development, either in a cell type relevant to the disease under investigation or in an entirely unrelated tissue. (ahajournals.org)
  • Efficient derivation of functional floor plate tissue from human embryonic stem cells. (springer.com)
  • Therefore, the regulation of H19 gene expression appears to be critical for cell fate decisions and tissue homeostasis. (pubmedcentralcanada.ca)
  • gene
  • Each plot shows single cells (dots) embedded in low-dimensional space on the basis of their RNA profile, based on predefined gene signatures ( A ) or PCA ( B , C ), highlighting distinct dynamic processes. (elifesciences.org)
  • Endoderm-Specific Gene Expression in Embryonic Stem Cells Differentiated to Embryoid Bodies," Exp Cell Res. (patentgenius.com)
  • Ube3a is a neuron-specific imprinted gene with an expression pattern that changes from biallelic to maternal only ( Ube3a imprinting) during differentiation. (springer.com)
  • We measured the relative expression levels of these 10 gene markers and assessed the Ube3a imprinting status of 54 neuron samples differentiated under various in vitro conditions. (springer.com)
  • Transfection experiments, using a green fluorescent protein reporter gene, driven by the myeloid-specific promoter, CD11b, demonstrated that ES Mø can also be used to study macrophage-restricted gene expression in vitro. (ahajournals.org)
  • It therefore would be of value to have a cellular system that permits the results of gene inactivation to be studied in vitro, bypassing the requirement for normal growth and development of an entire animal. (ahajournals.org)
  • Using mouse embryonic stem cells (mESCs), mammalian cell lines, Xenopus embryos and in vitro systems, we find that p120-catenin directly binds the REST-CoREST complex, displacing it from established gene targets to permit their transcriptional activation. (biologists.org)
  • In contrast, gene promoters show more uniform modifications across cell types. (biomedcentral.com)
  • embryogenesis
  • It plays an important role in cell survival, actin cytoskeleton organization and other processes in embryogenesis. (ijbs.com)
  • earliest events
  • 1 The subsequent penetration of these cells into the intima of the artery wall and their conversion to cholesterol-loaded foam cells mark one of the earliest events detectable in human atherosclerotic lesions. (ahajournals.org)
  • functional
  • The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of bone and vascular diseases. (hindawi.com)
  • The role of PI3K/AKT, MAPK/ERK and NF kappa beta signaling in the maintenance of human embryonic stem celll pluripotency and viability highlighted by transcriptional profiling and functional analysis," Human Molecular Genetics15:1460-2083,2006. (patentgenius.com)
  • Design and formulation of functional pluripotent stem cell-derived cardiac microtissues. (nih.gov)