• An electrophoretic mobility shift assay (EMSA) or mobility shift electrophoresis, also referred as a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common affinity electrophoresis technique used to study protein-DNA or protein-RNA interactions. (wikipedia.org)
  • Ensaios in vitro de interação proteína-DNA (EMSA), usando DNA telomérico simples fita Te11, dupla fita. (unesp.br)
  • Electrophoretic mobility shift assays (EMSA) were done in order to test the ability of each mutant to bind in vitro the G-rich telomeric DNA, double-stranded telomeric DNA and kDNA. (unesp.br)
  • EMSA and competition assays confirmed that LaRbp38 has at least one DNA binding domain (DBD), centrally located, between amino acid residues 141 and 235. (unesp.br)
  • Through deletion and mutation analysis of CPT1A's promoter combined with electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) experiments, the CPT1A promoter region (-50 to -5 nt) was determined to contain two putative Sp1 binding sites, namely Sp1a and Sp1b, which might act as the GBE regulation response DNA element. (biomedcentral.com)
  • In addition, nucleotide deletion and mutation analysis of a CPT1A promoter combined with electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) experiments were conducted to identify the specific responsive element of GBE and its flavonoids. (biomedcentral.com)
  • The evidence of a relatively high abundance of TFs, leading to increased expression of cochlin predicted by bioinformatic and biochemical analyses (EMSA and FBA), was further supported by TNT and promoter mutation TNT assays. (utmb.edu)
  • STUDY DESIGN: We tested our hypothesis in a carrageenan-induced thrombosis mouse model in vivo and lipopolysaccharide (LPS)-induced human endothelial cells (HUVECs) in vitro. (bvsalud.org)
  • By screening for enzyme activity using an in vivo biosensor assay, we subsequently discovered a G. sulfurreducens GGDEF enzyme that makes cGAMP, which is the founding member of Hypr GGDEF enzymes. (utah.edu)
  • We have analyzed the activity of cyclic di-GMP and cyclic di-AMP signaling enzymes using in vivo biosensor assays. (utah.edu)
  • The down-regulation of let-7d in IPF and the profibrotic effects of this down-regulation in vitro and in vivo suggest a key regulatory role for this microRNA in preventing lung fibrosis. (nih.gov)
  • Glucocorticoids used to treat inflammatory conditions are not yet recommended for HSV-1 therapy, since they have been reported to prolong viral shedding both in vivo and in vitro. (endocrinology-journals.org)
  • Analyses of the human AHR proximal promoter in MCF-7 human breast cancer cells using luciferase assays and electrophoretic mobility shift assays revealed multiple specificity protein (Sp) 1 binding sequences that are transcriptional activators in vitro. (umn.edu)
  • This method is referred to as a supershift assay, and is used to unambiguously identify a protein present in the protein - nucleic acid complex. (wikipedia.org)
  • RNase cleavage assay revealed that sco6808 transcripts, one of the potential regulators, are cleaved by AbsB. (snu.ac.kr)
  • From the detailed analyses by reporter assays, the EMSAs, and ChIP assays, we determined the functional PPRE of the human adrp gene. (biomedcentral.com)
  • However, we observed decreased DNA-binding affinity by the NR5A1 variant, while dual-luciferase reporter assays showed that the mutant effectively downregulated the transactivation capacity of anti-Müllerian hormone. (biomedcentral.com)
  • Native and full length LaTRF and a mutant bearing the putative Myb-like domain expressed in bacteria bound double-stranded telomeric DNA in vitro . (biomedcentral.com)
  • Biochemical analysis included electrophoretic mobility shift assays (EMSAs), filter binding assays (FBAs), coupled in vitro transcription-translation (TNT) assays and promoter mutation analysis. (utmb.edu)
  • In vitro functional analyses of the novel variant suggested no impairment to NR5A1 mRNA or protein expression relative to wild-type, and immunofluorescence confirmed similar localization of NR5A1 mutant to the cell nucleus. (biomedcentral.com)
  • In functional assays, DR8 and IR0 elements act as independent RAREs, whereas DR0 does not. (nih.gov)
  • Functional assays confirmed impaired DNA binding of mutated RBPJ, placing it among other notch-pathway Selleckchem Galardin proteins altered in human genetic syndromes. (phosphorylase-signal.com)
  • A mobility shift assay is electrophoretic separation of a protein-DNA or protein-RNA mixture on a polyacrylamide or agarose gel for a short period (about 1.5-2 hr for a 15- to 20-cm gel). (wikipedia.org)
  • However, assuming that the protein is capable of binding to the fragment, the lane with a protein that binds present will contain another band that represents the larger, less mobile complex of nucleic acid probe bound to protein which is 'shifted' up on the gel (since it has moved more slowly). (wikipedia.org)
  • An antibody that recognizes the protein can be added to this mixture to create an even larger complex with a greater shift. (wikipedia.org)
  • citation needed] Once DNA-protein binding is determined in vitro, a number of algorithms can narrow the search for identification of the transcription factor. (wikipedia.org)
  • SIGNIFICANCE STATEMENT A novel hybridization LC-MS/MS-based approach was successfully developed for the determination of ASO in vitro protein binding in plasma, and for the first time brain and CSF. (aspetjournals.org)
  • The hybridization LC-MS/MS platform provided unique advantages, including minimal matrix effects and high specificity, compared with traditional ligand binding assays or liquid scintillation approaches, which enabled efficient and reliable in vitro protein binding assay. (aspetjournals.org)
  • Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
  • Specific protein-binding measures are often used as assays in diagnostic assessments. (lookformedical.com)
  • Several reports suggest that it can function as a protein-protein interacton domain and it was recently demonstrated that the PHD finger of p300 can cooperate with the adjacent BROMO domain in nucleosome binding in vitro. (embl-heidelberg.de)
  • Substantial progress has been made in testing species barriers for CWD by using transgenic mice expressing species-specific prion protein (PrP), by direct infection into new species, or by in vitro conversion assays. (cdc.gov)
  • Finally, using electrophoretic mobility shift assay, we have identified YYl-related factors that bind to two other transcription initiators in cellular genes. (princeton.edu)
  • This correlates with the finding that pre-elafin/trappin-2 and elafin bind to DNA in vitro and attenuate the expression of some P. aeruginosa virulence factors, namely the biofilm formation and the secretion of pyoverdine. (biomedcentral.com)
  • Variants of the competition assay are useful for measuring the specificity of binding and for measurement of association and dissociation kinetics. (wikipedia.org)
  • The method provides substantial improvements, including minimal matrix effects and high specificity when compared with previously used oligonucleotide ƒ u detection methods such as ligand binding assays or liquid scintillation. (aspetjournals.org)
  • Conclusions: Our experimental data from enzymatic assays, immunocytochemistry and inhibition experiments strongly suggest a key role of the GH5_5 endocellulase in cell wall dissolution by Orciraptor agilis . (megazyme.com)
  • In vitro, studies in human umbilical vein endothelial cells (HUVECs) and in silico network pharmacology analyses were performed to reveal the underlying mechanisms of GQP and determine the main components, targets, and pathways of GQP, respectively. (bvsalud.org)
  • RT-PCR assays revealed that the transcriptional levels of the rim genes were consistent with the changes in rimocidin production in the recombinant strains. (biomedcentral.com)
  • Cell culture experiments using the type II cell-like cell line, A549, tested whether lipid-soluble cigarette smoke components affected binding and activation of retinoic acid response elements in vitro . (biomedcentral.com)
  • An in vitro cell culture model shows that lipid-soluble components of cigarette smoke decrease retinoic acid response element activation. (biomedcentral.com)
  • High purity dyed, soluble Azo-CM-Cellulose for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Previously, NdgR was identified by DNA affinity capture assay (DACA) and mass spectrometric analysis as a global regulator that controls amino acids synthesis, quorum sensing and antibiotics production. (snu.ac.kr)
  • Circular dichroism spectroscopy revealed the ability of sequences of this GC-rich region to form guanine-quadruplex structures in vitro. (umn.edu)
  • Attempts to confirm the binding sequences by electrophoretic mobility shift assay led to the discovery of a co-regulator, PA1413, via co-immunoprecipitation assay. (diagenode.com)
  • RESULTS: Oral administration of 227.5 mg/kg, 445 mg/kg and 910 mg/kg of GQP significantly inhibited thrombi in the lung, liver, and tail and augmented tail blood flow of carrageenan-induced mice with reduced plasma tumor necrosis factor α (TNF-α) and diminished expression of high mobility group box 1 (HMGB1) in lung tissues. (bvsalud.org)
  • Recombinant RAR-RXR binds these non-canonical spacings in vitro with comparable affinities to DR2 and DR5. (nih.gov)
  • Mutation in the gene encoding this regulator abolished the ability of P. aeruginosa to produce biofilms in vitro, without any effect on the planktonic growth. (diagenode.com)
  • Although precursors can be found in earlier literature, most current assays are based on methods described by Garner and Revzin and Fried and Crothers. (wikipedia.org)
  • The rimR 2 deletion and complementation assays were conducted to explore its role. (biomedcentral.com)
  • Plasma lipoproteins are macromolecular complexes of lipids and proteins that are classified by density and electrophoretic mobility. (medscape.com)
  • Consensus sequence oligonucleotides for the transcription factor of interest will be able to compete for the binding, eliminating the shifted band, and must be confirmed by supershift. (wikipedia.org)
  • An RNA Binding Peptide Consisting of 4 Varieties of Amino Acid by in Vitro Choice Utilizing cDNA Show. (humgen.org)
  • By in vitro choice of binding peptides utilizing the cDNA show methodology, a attribute peptide was chosen from a random peptide library and assayed by electrophoretic mobility shift and pull-down assays. (humgen.org)
  • Thioglycollate-elicited murine peritoneal macrophages were cultured with CLX-090717 and lipopolysaccharide (LPS)-induced TNF-alpha release was assayed. (ox.ac.uk)
  • Gel shift assays are often performed in vitro concurrently with DNase footprinting, primer extension, and promoter-probe experiments when studying transcription initiation, DNA gang replication, DNA repair or RNA processing and maturation, as well as pre-mRNA splicing. (wikipedia.org)
  • OBJECTIVES: To evaluate the clinical efficacy of a novel synthetic peroxisome proliferator-activated receptor gamma (PPAR-gamma) agonist, CLX-090717, in several in vitro cell culture systems and murine CIA, an experimental model of RA. (ox.ac.uk)
  • Fig. 1: Overview of prime editing and feasibility studies in vitro and in yeast cells. (nature.com)
  • In vitro drug ƒ u results are one of the properties used to prioritize certain drug candidates into further stages of drug development. (aspetjournals.org)
  • The procedure for the assay of endo -Cellulase using Azo-CM-Cellulose (Liquid) is equivalent to this. (megazyme.com)
  • As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)
  • Using Co-IP assays and native PAGE we have shown a direct physical interaction between PRIN2 and CSP41b. (frontiersin.org)
  • We recently developed a biosensor-based platereader assay to quantitate cGAMP levels in DNA-stimulated mammalian cell lysates. (utah.edu)
  • Assays based on the use of these substrates are sensitive and highly specific for either alpha-amylase or (1→4)-β-D-glucanase. (megazyme.com)
  • Please note the video above shows the protocol for assay of endo -cellulase using Azo-CM cellulose. (megazyme.com)