Vitro differentiate into different cell types. Medical search. Frequent questions

To understand the various mechanisms driving disease, it is important to have a good model system in vitro that can recapitulate the in vivo disease process. (nature.com)
Mediates ex vivo expansion of cord blood CD34+ hematopoietic stem and progenitor cells (Huang et al. (stemcell.com)
Activation of OCT4 enhances ex vivo expansion of human cord blood hematopoietic stem and progenitor cells by regulating HOXB4 expression. (stemcell.com)
Although hematopoietic stem cells (HSC) are the best characterized and the most clinically used adult stem cells, efforts are still needed to understand how to best ex vivo expand these cells. (stemcell.com)
Hopes for the creation of a more stable transfusion system have centered on the establishment of a technology for large-scale erythrocyte production ex vivo, using for instance iPS cells or ES cells. (sciencedaily.com)
Increasingly, isolates representing single clones are needed for in vitro and in vivo research, including genomic, murine, and translational studies, to advance our understanding of chlamydial pathogenesis. (cdc.gov)
2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
Antibodies to LAG-3 inhibit suppression by induced Tregs both in vitro and in vivo. (nih.gov)
To further evaluate the implants' ability to reveal phenotypic changes associated with known pathologies in vivo, Paṣca's team implanted rats with hCOs from healthy cell lines as well as those with the neurodevelopmental disease Timothy Syndrome (TS). (bruker.com)
Thus, an in vivo model that can utilize patient-derived cells in combination with a fully formed nervous system can now be implemented in the study of neurological disorders. (bruker.com)
While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)
Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)
Most clinical trials which utilize ADSCs for OA treatment have been based on the autologous cells from the stromal vascular fraction, as have most of the in vivo studies ( 6 , 7 ). (spandidos-publications.com)
MDSC that lack CAT2 have reduced capacity to suppress T cell responses both ex vivo and in vivo, as evidenced by increased T cell expansion in prostatitis and decreased tumor growth in cancer models. (purdue.edu)
We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
Finally, we show that the novel anti-ageing drugs we uncovered in vitro , indeed increased longevity in vivo . (biorxiv.org)
Since my work in the U.S was cell culture based I am now engaging in broiler trials to determine if our results were representative of satellite cell activity in vivo in commercial birds. (poultryhub.org)
Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
While much is known about the ErbB receptor binding characteristics of BTC and its effect on a variety of cultured cells under different conditions, the challenge that lies ahead is to determine the role of BTC in vivo. (embl.de)
These cells can be stably expanded and produce myelin in vivo . (mpg.de)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
Mesenchymal stem cells clearly modulate immune responses, as demonstrated by both in vitro and in vivo studies. (vin.com)
This chapter provides a review on how pluripotency is defined and examined in vitro and in vivo. (edu.au)
In this thesis, human glia-to-neuron direct conversion and engineered viral vectors are explored using pre-clinical in vitro and ex vivo models. (lu.se)

Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
We alter the deficiency by infecting the SFTPB deficient iPSCs with a lentivirus carrying the wild type SFTPB gene. (nature.com)
Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
Signs of aging were erased and the iPSCs obtained can produce functional cells, of any type, with an increased proliferation capacity and longevity," explains Jean-Marc Lemaitre who directs the Inserm AVENIR team. (eurekalert.org)
The National Academies of Sciences, Engineering and Medicine have also released guidance for "Human Embryonic Stem Cell Research" (2005 and updated in 2010) which provides no guidance on induced pluripotent stem cells (iPSCs) [ 3 ], and "The emerging field of human neural organoids, transplants, and chimeras" (2021) which didn't include other stem cell-derived tissues and deliberately excluded transplantation of organoids in humans [ 4 ]. (biomedcentral.com)
To address these questions, particularly in a human-relevant system, we have focused upon the use of human induced pluripotent stem cells (iPSCs) to assess their ability to give rise to RGCs, with a particular emphasis upon ipRGC differentiation. (indianactsi.org)
We found that iPSCs can be effectively differentiated into retinal cells, including the formation of 3D retinal organoids. (indianactsi.org)
My project will highlight how differentiating iPSCs into functional ipRGCs will allow for more accurate ophthalmological disease modeling in vitro. (indianactsi.org)
Finally, as a first step to establish a relevant in vitro model of SYS, we reprogrammed fibroblasts from different patients to induced pluripotent stem cells (iPSCs), which can be then differentiated to relevant neural cell types and brain organoids to further study the pathophysiological mechanisms underlying this disease. (ub.edu)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)
Skin cells are taken from a patient, reprogrammed to induced pluripotent stem cells (iPSCs), differentiated into dopaminergic progenitors, and put back in the patient. (lu.se)

Studies have shown that the proliferation rate of DPSCs is 30% higher than in other stem cells, such as bone marrow stromal stem cells (BMSSCs). (wikipedia.org)
Overexpression of this gene is associated with tumor cell proliferation and metastasis. (cancerindex.org)
Transwell migration, cell cycle analysis, MTT and colony formation assays were performed to determine the effect of DRAM2 overexpression and knockdown on NSCLC-cell migration and proliferation. (cancerindex.org)
Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)
This loss in the glucose stimulated insulin secretion (GSIS) phenotype correlates with changes in morphology, increased proliferation and increased alkaline phosphatase (AP) activity, suggesting that the loss of GSIS may be due to de-differentiation of the high passage MIN6 cells. (dcu.ie)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
We discovered that myocilin increased cell proliferation and survival. (nih.gov)
Researchers first multiplied skin cells (fibroblasts) from a 74 year-old donor to obtain the senescence characterized by the end of cellular proliferation. (eurekalert.org)
These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. (rndsystems.com)
The upregulation of miR‑145 and miR‑221 was associated with the enhanced proliferation of periosteal cells and chondrogenic potential, respectively. (spandidos-publications.com)
A one hour exposure to limonene + ozone resulted in decreased proliferation when compared to cells exposed to limonene alone. (cdc.gov)
Post-hatch muscle hypertrophy, the subject of Dean's work, occurs through the proliferation and differentiation of myogenic satellite cells into muscle myofibres, which then fuse with existing muscle myofibres to increase their size. (poultryhub.org)
Among their other immunological properties, MSC inhibit lymphocyte proliferation and cytokine production, suppress dendritic cell function and alter DC cytokine production, and decrease IFN-g production by NK cells. (vin.com)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)
AU - Lee,W T, AU - Pelletier,W J, PY - 1998/9/23/pubmed PY - 1998/9/23/medline PY - 1998/9/23/entrez SP - 1 EP - 11 JF - Cellular immunology JO - Cell Immunol VL - 188 IS - 1 N2 - Stimulation of naive CD4 cells by specific antigen results in proliferation and changes in cell surface marker expression as the cells differentiate into effector and memory cells. (unboundmedicine.com)

Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)

By transducing two genes (c-MYC and BCL-XL) into iPS cells and ES cells, a research team has succeeded in producing erythrocyte progenitor cells with almost unlimited ability to replicate in vitro, which they then differentiated successfully into mature erythrocytes. (sciencedaily.com)
Erythrocytes are produced in the body by progressive evolution of hematopoietic stem cells into hematopoietic progenitor cells and then erythroblasts. (sciencedaily.com)
During the differentiation process from hematopoietic progenitor cells, chromosomes in the cell nucleus aggregate and the cell becomes a mature erythrocyte by subsequently losing this nucleus (enucleation). (sciencedaily.com)
Hematopoietic progenitor cells, however, still have self-replication ability, and if the factors involved in this self-replication could be identified, it might become possible to induce virtually unlimited replication of progenitor cells. (sciencedaily.com)
The research showed that an increase in the expression of the c-MYC gene led to an enhancement of the replication ability of erythrocyte progenitor cells. (sciencedaily.com)
It was also found that the expression of the BCL-XL gene was higher during the maturation process of erythrocyte progenitor cells than during their replication process. (sciencedaily.com)
As the c-MYC gene and BCL-XL gene are present within erythrocyte progenitor cells, methods that make use of them actually mimic the body's own system and can therefore be thought of as safer with a view to future clinical application than the previously developed systems for large-scale erythrocyte production using oncogene-derived from Human papillomavirus that are not normally expressed in the human body. (sciencedaily.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
Generation of induced oligodendrocyte progenitor cells (iOPCs) from somatic fibroblasts may provide a cell-based therapeutic strategy against myelin diseases. (mpg.de)
These cells differentiated from induced oligodendrocyte progenitor cells (iOPCs), which in turn arose from pericytes (blue: cell nuclei). (mpg.de)
Pericytes and oligodendrocyte progenitor cells originate from the same cell population during development,' said Kee-Pyo Kim, the first author of the study. (mpg.de)
Therefore, we assumed that pericytes would be well suited for this reprogramming and subsequent differentiation into oligodendrocyte progenitor cells,' Kim continued. (mpg.de)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

The different cell populations, however, differ in certain aspects of their growth rate in culture, marker gene expression and cell differentiation, although the extent to which these differences can be attributed to tissue of origin, function or culture conditions remains unclear. (wikipedia.org)
Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
The resulting differentiated populations were analysed for transcript expression of a range of multi-lineage markers i e neuronal, myogenic, exocrine and endocrine pancreas, extra-embryonic and apoptotic markers. (dcu.ie)
Two of these papers redefine endodermal subtypes derived from hESCs, including new methods to isolate lineage restricted endodermal populations and a means to distinguish between single endodermal cells. (ca.gov)
Fibrotic livers are characterized by an inflammatory microenvironment that is composed of various immunologically active cells, including liver-resident populations (e.g. (mdpi.com)
We propose that LAG-3 marks regulatory T cell populations and contributes to their suppressor activity. (nih.gov)
In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)

However, the rejection reaction often occurs in the transplantation of MSCs and could induce cell death. (nih.gov)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
We are interested in investigating the potential neuroprotective benefits of stem cell transplantation, which has produced encouraging results in different models of CNS degeneration. (nih.gov)
Transplantation of living nuclei from blastula cells into enucleated frogs' eggs. (springer.com)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Surprisingly, the iOPCs retain the memory of their original cell identity, which prevents them from differentiating into myelin-producing oligodendrocytes in mice after transplantation. (mpg.de)
However, after the iOPCs were further differentiated into pre-oligodendrocytes in vitro before their transplantation, they formed myelin. (mpg.de)
This involves transplantation of developing midbrain cells from aborted fetuses, (the part that form mesDA neurons), into the striatum of a PD patient. (lu.se)
We still do not know what specific factors contribute to the success in transplantation i.e. what cells are responsible for motor recovery? (lu.se)
The aim of this thesis was to understand how particular factors such as neuronal content, placement and cell source, affect functional outcome after transplantation into the rodent brain. (lu.se)
My projects investigated whether patient-derived cells are more prone to develop disease phenotypes and what can be done to protect these cells after transplantation," explained Fredrik. (lu.se)
Stem cell transplantation is the removal of stem cells (undifferentiated cells) from a healthy person and their injection into someone who has a serious blood disorder. (msdmanuals.com)
Overview of Transplantation Transplantation is the removal of living, functioning cells, tissues, or organs from the body and then their transfer back into the same body or into a different body. (msdmanuals.com)
But researchers think that these stem cells have the most potential for producing different kinds of cells and for surviving after transplantation. (msdmanuals.com)
These stem cells are most often used for transplantation. (msdmanuals.com)

They are pluripotent, as they can form embryoid body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. (wikipedia.org)
DPSCs can differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers. (wikipedia.org)
This research paves the way for the therapeutic use of iPS, insofar as an ideal source of adult cells is provided, which are tolerated by the immune system and can repair organs or tissues in elderly patients. (eurekalert.org)
There is no disagreement among experts about the capacity of (ESCs) to form any and all cells and tissues of the body,' Groopman declares. (freerepublic.com)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
In July 2022, the European Union released a Proposal [ 5 ] to significantly change its regulation of cells and tissues and repeal Directive 2004/23/EC, the cornerstone of such regulation for the last two decades. (biomedcentral.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Recent years have brought increased interest in the potential for adult stem cells to help in the treatment of many diseases through both their regenerative properties as well as their apparent ability to alter the environment in injured and diseased tissues. (vin.com)
Adult stem cells can be obtained from many differentiated tissues including but not limited to bone marrow, bone, fat, and muscle. (vin.com)
1,2 Cancer organoids have been generated from a range of human cancer tissues and cell lines including colon, pancreas, prostate, liver, breast, bladder and lung. (technologynetworks.com)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
Researchers hope to use stem cells to repair or replace cells or tissues damaged or destroyed by such disorders as Parkinson disease, diabetes, and spinal injuries. (msdmanuals.com)

Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
This type of stem cell is very versatile and has a wide range of uses, though procuring them entails medical and ethical challenges. (articlecity.com)
This piece will focus on the medical technology being developed using Wharton's jelly as a source material for stem cells, but will also delve into broader aspects of stem cell research, one of the most fascinating current areas of scientific study. (articlecity.com)
Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
The latest advances in the field of spinal cord development and its regeneration following damage were discussed at a recent EMBO workshop 'Spinal cord development and regeneration' in Sitges, Spain (October, 2014), highlighting the use of direct visualization of cellular processes, genome-wide molecular techniques and the development of methods for directed stem cell differentiation and regeneration. (biologists.com)
R&D Systems offers a wide range of products to support pluripotent stem cell culture and differentiation. (rndsystems.com)
In addition, R&D Systems offers a variety of products to assess differentiation status and identify specific stem cell types of interest, including panels of marker antibodies, primer pairs, multi-color flow cytometry kits, and specialized verification kits. (rndsystems.com)
However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)
The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
Embryonic stem cell lines derived from human blastocysts. (springer.com)
Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
So it is with their current combined attack on adult stem cell research, designed to support the alternative of embryonic stem cells . (freerepublic.com)
The result says Chris Mooney in the Washington Monthly is that 'conservatives have latched onto fringe science in order to advance moral arguments' by embracing adult stem cell research. (freerepublic.com)
Amazingly, there are also more than 300 adult stem cell clinical trials . (freerepublic.com)
Embryonic stem cell propagandists will tell you adult stem cell research had a huge head start and embryonic stem cells only need time (and more importantly, massive government funding) to catch up. (freerepublic.com)
Yet as a new book called The Proteus Effect points out, both types of stem cell research date back half a century. (freerepublic.com)
Billerica, Massachusetts - October 18, 2022: Researchers at Stanford University are the first team to successfully transplant stem cell-derived human cortical organoids (hCOs) into developing rat somatosensory cortex. (bruker.com)
Still, there has been a demand for using these stem cell-derived organoids to look at circuit function in a fully intact nervous system. (bruker.com)
To this end, we overexpressed key transcriptional regulators of astroglial development in human GBM and GBM stem cell lines. (lu.se)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Stem cell therapy raised hope of overcoming these issues, but created new risks including tumour formation and limited benefit if the desired target tissue does not form. (biomedcentral.com)
This article details the potential health risks of 3-dimensional organoid and tissue therapy versus dissociated stem cell therapy. (biomedcentral.com)
The current ethical and regulatory issues surrounding 3-dimensional organoid and tissue therapy are presented with a focus on the highly influential FDA and International Society of Stem Cell Research (ISSCR) guidelines. (biomedcentral.com)
Stem cell therapy is one approach to regenerative medicine. (biomedcentral.com)
It is therefore expected that clinical trials implanting more complex 3-dimensional stem cell-derived tissue constructs (SCTCs) in humans will occur in the near future, including a recent report of autologous intestinal epithelial organoids being implanted into a patient with ulcerative colitis (jRCTb032190207) [ 2 ]. (biomedcentral.com)
As the regulations and guidance around this technology are limited, reducing the benefit in comparing regulations across jurisdictions, we will focus on the FDA and International Society of Stem Cell Research (ISSCR) communications, as they have been widely influential in this area. (biomedcentral.com)
The study, published in Cell Stem Cell, presents a cell-based approach for studying the potential of therapies against myelin diseases. (mpg.de)
Stem cell therapy in particular is an innovative new field of scientific investigation and clinical application that holds promise for a variety of diseases in veterinary medicine as well as human medicine. (vin.com)
A stem cell is a generic term referring to any unspecialized cell that is capable of long-term self-renewal through cell division but that can be induced to differentiate into a specialized, functional cell. (vin.com)
For most studies, the adult stem cell in question is actually a mesenchymal stem cell or mesenchymal stromal cell. (vin.com)
In addition, it is also important to keep the immune modulatory properties of MSC in mind when interpreting the results of many stem cell therapy studies. (vin.com)
Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)
and stem cell regeneration. (lu.se)
Today, clinical trials using stem cell-derived dopaminergic progenitors have commenced. (lu.se)
How did you end up doing a Ph.D. at Lund Stem Cell Center? (lu.se)
During a biology course, we had a guest speaker from Lund Stem Cell Center who talked about stem cells and their potential to become any type of cell in the body. (lu.se)
I found it incredibly interesting so when it came to doing a master's project, I reached out to Lund Stem Cell Center. (lu.se)
What have you found the most enjoyable during your Ph.D. studies at Lund Stem Cell Center? (lu.se)

Dental pulp stem cells (DPSCs) are stem cells present in the dental pulp, which is the soft living tissue within teeth. (wikipedia.org)
As a result, DPSCs have been thought of as an extremely promising source of cells used in endogenous tissue engineering. (wikipedia.org)
These characteristics of DPSCs are mainly due to the fact that they exhibit elevated amounts of cell cycling molecules, one being cyclin-dependent kinase 6 (CDK6), present in the dental pulp tissue. (wikipedia.org)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
The meeting, organized by Elisa Martí (IBMB-CSIC, Barcelona, Spain), Jonas Muhr (Karolinska Institutet, Stockholm, Sweden) and Kate Storey (University of Dundee, UK), brought together international researchers interested in spinal cord development and those who are developing strategies to restore the function of the spinal cord in pathological situations using regeneration of the damaged tissue, including controlled cell replacement. (biologists.com)
It's well established that embryonic stem cells can generate any kind of tissue found in the body,' Mooney writes flatly. (freerepublic.com)
But we already know embryonic cells cannot generate placental tissue . (freerepublic.com)
In aggregate, our study and various others do support the idea that one (ASC) can give rise to all types of tissue,' said Ira Black, the head of one of those labs. (freerepublic.com)
The Stanford team, led by Sergiu Paṣca were able to grow and differentiate transplanted cells alongside the developing rat tissue, generating robust cellular diversity, and establishing meaningful functional connections throughout the brain. (bruker.com)
In 2015, Stanford researchers began to generate three-dimensional clusters of cells of neural tissue, known as cortical organoids . (bruker.com)
In their latest research, they were finally able to implant these organoids into a developing rat nervous system, allowing the human tissue to fully differentiate and develop, integrating with the rat tissue around it. (bruker.com)
Adipose tissue-derived stem cells (ADSCs) have been characterized as having the ability to self-renew and differentiate into different connective tissue cells, including osteoblasts, adipocytes, chondrocytes and myocytes, under specific inductive stimuli ( 4 ). (spandidos-publications.com)
In total, 1-10% of nucleated cells in adipose tissue are ADSCs, whereas only 0.0001-0.01% of nucleated cells in bone marrow are stem cells ( 5 ). (spandidos-publications.com)
Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
The studies described here characterize an in vitro model using an epithelial cell line (A549) or differentiated epithelial tissue (MucilAir). (cdc.gov)
XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
Stem cells can be obtained from a donor or be autologous, eliminating any issues from the use of animals in tissue harvesting. (biomedcentral.com)
In this review, we provide an up-to-date overview of the current knowledge of how A. rhizogenes induces root formation, on the new uses for A. rhizogenes in tissue culture and composite plant production (wild-type shoots with transgenic roots), and the recent development of a disarmed version of A. rhizogenes for stable transgenic plant production. (bioone.org)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
Mesenchymal stem cells are multipotent but not pluripotent, which means they can differentiate into some, or "multiple," but not all tissue types. (vin.com)
1 Defined as a three-dimensional multicellular in vitro tissue construct, organoids are derived from cells that spontaneously self-organize into properly differentiated functional cell types to mimic at least some function of an organ. (technologynetworks.com)
Organoids can be derived from pluripotent stem cells (including embryonic stem cells or induced pluripotent stem cells) or neonatal or adult stem cells from healthy or diseased tissue. (technologynetworks.com)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
Atrophic gastritis is a histopathologic entity characterized by chronic inflammation of the gastric mucosa with loss of gastric glandular cells and replacement by intestinal-type epithelium, pyloric-type glands, and fibrous tissue. (medscape.com)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)
Use of human fetal tissue raises several ethical issues, but are there alternative cell sources that can substitute effectively? (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)

DPSCs were found to be able to differentiate into adipocytes and neural-like cells. (wikipedia.org)
Recent years have witnessed a great advancement in our understanding of the regulation of these adipocytes and an increased appreciation of the potential these cells have to counteract obesity. (umich.edu)
Most importantly stem cells possess the ability to differentiate into cell types of multiple lineages including adipocytes, chrondrocytes, and osteocytes. (vin.com)

D) Representative flow cytometry plots of cells stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD71 and phycoerythrin (PE)-conjugated anti-CD235a antibodies, demonstrating sequential expression of CD71 followed by CD235a and subsequent loss of CD71. (haematologica.org)
Additionally cell surface marker characterization via flow cytometry differentiates them from hematopoetic cells, though no truly unique MSC molecule has been identified. (vin.com)

Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
The results represent significant progress for research into iPSC cells and a further step forwards for regenerative medicine. (eurekalert.org)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
Stem cells and regenerative medicine - future perspectives. (springer.com)
Stem cells in regenerative medicine: introduction. (springer.com)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)
Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)

Bone marrow mesenchymal stem cells (MSCs) have been identified to have the potential to differentiate into multiple types of cells. (nih.gov)
We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
Tumor site monocytic-MDSC lacked the ability to differentiate into other cells types such as osteoclasts and granulocytic-MDSC, whereas the bone marrow and spleen counterparts could form both cell types. (purdue.edu)
Induction of p53 signaling elevated the differentiation of the bone marrow MDSC into granulocytic cell types. (purdue.edu)
Blood is preferred to bone marrow as a source because the procedure is less invasive and the number (count) of blood cells returns to normal more quickly. (msdmanuals.com)
The bone marrow and blood of children and adults contain stem cells. (msdmanuals.com)

Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)

Inserm's AVENIR "Genomic plasticity and aging" team, directed by Jean-Marc Lemaitre, Inserm researcher at the Functional Genomics Institute (Inserm/CNRS/Université de Montpellier 1 and 2), has recently succeeded in rejuvenating cells from elderly donors (aged over 100). (eurekalert.org)
Using this new "cocktail" of six factors, the senescent cells, programmed into functional iPSC cells, re-acquired the characteristics of embryonic pluripotent stem cells. (eurekalert.org)
Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
Bruker offers differentiated, high-value life science and diagnostics systems and solutions in preclinical imaging, clinical phenomics research, proteomics and multiomics, spatial and single-cell biology, functional structural and condensate biology, as well as in clinical microbiology and molecular diagnostics. (bruker.com)
Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
4,6,7 It has been proposed that some of these MSC may actually differentiate into functional renal tubular epithelial cells, though this theory remains controversial. (vin.com)
Because the disease-causing mutations in ATP2A2 affect functional domains of the gene, the mechanism of autosomal dominant transmission is believed to be haploinsufficiency, in which a single wild-type functioning ATP2A2 is insufficient to prevent disease. (medscape.com)
Shane Grealish: Cell Replacement Therapy for Parkinson's Disease: The Importance of Neuronal Subtype, Cell Source and Connectivity for Functional Recovery. (lu.se)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

Others are already partially differentiated and can only become, for example, some kinds of nerve cell. (msdmanuals.com)

It is essential to have sensitive and reproducible methods of either quantifying or isolating these stem cells and progenitors to understand their intrinsic properties and how extrinsic signals regulate their development. (karger.com)
However, stem cells are difficult to distinguish from multipotential progenitors, which may look and act like them. (karger.com)
Next, in order to study the potential of autologous cell replacement therapy we transplanted progenitors derived from a PD patient into a pre-clinical rat model. (lu.se)

It is a group of optic neuropathies characterized by the death of retinal ganglion cells (RGCs), leading to a specific deformation of the optic nerve head. (nih.gov)
Intrinsically photosensitive retinal ganglion cells (ipRGCs) are a subset of retinal ganglion cells that respond to light independently from rod and cone photoreceptor input. (indianactsi.org)
Retinal ganglion cells can be purified and matured in vitro to generate models for optic neuropathies. (indianactsi.org)

In the search for improved and high-throughput in vitro models, organoids have emerged as a promising 3D cell culture technology. (technologynetworks.com)

We have identified a new motif, termed SMAD Complex Associated (SCA) that is bound by SMAD2/3/4 and FOXH1 in human embryonic stem cells (hESCs) and derived endoderm. (ca.gov)
To elucidate the Nodal transcriptional network that governs endoderm formation, we used ChIP-seq to identify genomic targets for SMAD2/3, SMAD3, SMAD4, FOXH1 and the active and repressive chromatin marks, H3K4me3 and H3K27me3, in human embryonic stem cells (hESCs) and derived endoderm. (ca.gov)
The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)

In addition, they do not require feeder layers and can differentiate into many neural lineages under the appropriate conditions (1). (ddw-online.com)
First, it was tested whether patient‑derived ADSCs could differentiate into chondrogenic and osteogenic lineages. (spandidos-publications.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)

Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
In particular, adult stem cells called mesenchymal stem cells can migrate to affected areas and may be able to support the growth of other stem cells as well as moderate the response of the immune system. (vin.com)
Mesenchymal stem cells or mesenchymal stromal cells (MSC) are plastic adherent and assume a fibroblast-like morphology during culture. (vin.com)
Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)

Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)
Finally an outline of disease-specific pluripotent stem cells is reviewed within the context of their capacity to generate the cell type of interest. (edu.au)

We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
And while in human pluripotent stem cells, miRNAs have been directly linked to the core pluripotency network, including the cell cycle regulation and the maintenance of the self-renewing capacity, their role in the onset of differentiation in other contexts, such as determination of neural cell fate, remains poorly described. (muni.cz)

Oct-4 (POU5F1) is a transcription factor that is critically involved in the self-renewal of pluripotent stem cells, and its expression is commonly used as a marker for pluripotency. (stemcell.com)
Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
Although ES cells may be ideal in terms of their pluripotency, from a therapeutic point of view, their great disadvantage is that they are not patient derived. (edu.au)
The various ways in which pluripotent stem cells are generated, particularly in relation to induced pluripotency, are also discussed. (edu.au)

SIRT1 directly regulated MSCs into osteoblasts which then shows the accumulation of significantly higher levels of calcium after osteogenic differentiation in vitro, suggesting the potential role of DPSCs in enhancing the efficiency of DO. (wikipedia.org)

In addition, inflammation‑inflicted oxidative stress was induced in vitro by stimulating chondrocytes with H2O2. (spandidos-publications.com)

The murine embryonic stem (ES) cell line, ES-D3 was used in this project as a model for (a) investigating controversial aspects of current protocols used for the derivation of (3 cell types from ES cells and (b) investigating the potential to regulate the differentiation of ES cells into endodermal derivatives 1 e 0-1 ike cells, using extra-cellular factors previously associated with various aspects of pancreatic development. (dcu.ie)
Induced pluripotent stem cells, which harness the power of genetic reprogramming - basically, the altering of a cell's DNA - to change the course of cellular development. (articlecity.com)
We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
At the cellular level, several processes are highly controlled, including the establishment and maintenance of apico-basal polarity, the control of the cell cycle and cell cycle exit, and the acquisition of cell subtype identities. (biologists.com)
The researchers have successfully rejuvenated cells from elderly donors, some over 100 years old, thus demonstrating the reversibility of the cellular aging process. (eurekalert.org)
They have the physiological characteristics of "young" cells, both from the perspective of their proliferative capacity and their cellular metabolisms. (eurekalert.org)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
This full potentiality makes them particularly suitable for developing cell replacement therapies or establishing cellular model systems. (edu.au)
3,4 The zygote and cells derived from the first two cellular divisions constitute the most primitive cells (totipotent cells) that are capable of forming the embryo and the embryonic annexes (e.g. placenta, amniotic membranes etc). (bvsalud.org)

After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
We also show the presence of normal lamellar bodies and the secretion of surfactant into the cell culture medium in the organoids of lentiviral infected cells. (nature.com)
Their work " Maturation and circuit integration of transplanted human cortical organoids " was published by Nature on October 12, 2022, and demonstrates that the human-derived cells fully integrate into the rat nervous system. (bruker.com)
These organoids were promising as a method of in vitro analysis of human disorders, as they develop into differentiated cell types (including glia). (bruker.com)
In this article, we explore the various types of organoids under development and shine a spotlight on some of the different approaches to organoids in cancer research. (technologynetworks.com)
This year, a research group led by Hongjun Song, Professor of Neuroscience at the Perelman School of Medicine at the University of Pennsylvania, published a report in Cell detailing methods for the rapid generation of patient-derived glioblastoma organoids. (technologynetworks.com)

Generation of reprogrammed somatic cells, known as induced pluripotent stem (iPS) cells, was initially performed by ectopic expression of four transcription factors, however, now this process is becoming more streamlined by improved methods. (edu.au)

The involvement of microRNAs (miRNAs) in orchestrating self-renewal and differentiation of stem cells has been revealed in a number of recent studies. (muni.cz)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

The properties of these cells exhibited in this study suggest that OST-SHED could potentially prevent of oxidative stress-induced brain damage and could aid in the development of therapeutic tools for neurodegenerative disorders. (wikipedia.org)
Oppositely, expression of mutated myocilin sensitizes cells to apoptosis induced by oxidative stress. (nih.gov)

Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. (lww.com)
1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
Pericytes coat capillary endothelial cells and form an integral part of the blood-brain barrier. (mpg.de)

Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
In close cooperation with our customers, Bruker is enabling innovation, improved productivity and customer success in life science molecular and cell biology research, in applied and pharma applications, in microscopy and nanoanalysis, as well as in industrial applications. (bruker.com)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
More recently, advances in medical biology have shown that the genomic state of a somatic cell can be altered or 'reprogrammed' to become pluripotent. (edu.au)

A comparative analysis of gene expression arrays from antigen-specific CD4(+) T cells differentiating to either an effector/memory or a regulatory phenotype revealed Treg-selective expression of LAG-3, a CD4-related molecule that binds MHC class II. (nih.gov)
Natural CD4(+)CD25(+) Tregs express LAG-3 upon activation, which is significantly enhanced in the presence of effector cells, whereas CD4(+)CD25(+) Tregs from LAG-3(-/-) mice exhibit reduced regulatory activity. (nih.gov)
Lastly, ectopic expression of LAG-3 on CD4(+) T cells significantly reduces their proliferative capacity and confers on them suppressor activity toward effector T cells. (nih.gov)
Phenotypic changes could also be observed as the cells differentiated into effector/memory cells. (unboundmedicine.com)

They are able to differentiate into any cell of an organism and have the ability of self-renewal. (articlecity.com)
Stem cells are defined by their capacity for self-renewal and multilineage differentiation, making them uniquely situated to treat a broad spectrum of human diseases. (articlecity.com)

In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders. (rndsystems.com)
We developed a novel shotgun cell culture harvest assay for this purpose because we found that recent clinical samples do not form plaques. (cdc.gov)
The number of clinical trials involving embryonic stem cells? (freerepublic.com)
Clinical trials have been run for the implantation of stem cells and their derived products. (biomedcentral.com)
But the reprogramming process is inefficient, and the resulting cells do not proliferate and differentiate sufficiently to have use in clinical practice. (mpg.de)
The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Many anticipate that these cell culture models will result in more efficient translation of research into clinical success. (technologynetworks.com)
En route to the patient we can also remove disease-associated genes that might compromise the transplanted cells and eventually the clinical benefit of the graft over time," described Fredrik. (lu.se)

We have made significant progress during the previous granting period which has resulted in a publication in Genome Research detailing genomic DNA methylation changes in a variety of human embryonic stem cells and their derivatives. (ca.gov)

Although these erythrocytes consisted mostly of fetal-type hemoglobin, they were confirmed to have oxygen-carrying capacity and to have circulatory capacity following transfusion into mice. (sciencedaily.com)
Viable offspring derived from fetal and adult mammalian cells. (springer.com)

Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. (rndsystems.com)

Mouse embryonic fibroblasts may be used to maintain and expand pluripotent stem cells in an undifferentiated state. (rndsystems.com)

I also describe the development of a novel behavioural task that is predictive of mesDA neuron cell loss in mice. (lu.se)

Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. (springer.com)
By triggering certain genes, researchers may be able to cause the stem cells to specialize and become the cells that need to be replaced. (msdmanuals.com)
One way to induce these cells is to inject them with material that affects their genes, a process called reprogramming. (msdmanuals.com)

We observed a very significant decline in satellite cell activity as our restrictions became more stringent, which was reflected in their reduced myogenic marker gene expression. (poultryhub.org)
Mature oligodendrocytes (red marker: myelin basic protein) in an in vitro culture. (mpg.de)
Several of the marker changes (e.g., differences in CD45RB, CD44, and L-selectin levels) appear to be relatively stable and permit the identification of memory T cells. (unboundmedicine.com)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)

The primary objective of this study was to verify whether a natural antioxidant, lycopene, could protect MSCs from ischemia-induced apoptosis in vitro. (nih.gov)

Applying 2-stage regulated differentiation protocols developedduring the course of the study, we demonstrated the derivation of an intermediate multi-potential population (RAJEBs) from undifferentiated ES cells that preferentially gave rise to pancreatic endocrine insulin-expressing cell types in the presence of SB and neuronal-like cell types in the presence of AA or BTC. (dcu.ie)
Patient-derived cultured cells can only answer basic questions of neuronal dysfunction, as they are never able to develop into fully functioning circuits. (bruker.com)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)
Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)

Morphologically, A. rhizogenes -induced hairy roots are very similar in structure to wild-type roots with a few notable exceptions: Root hairs are longer, more numerous, and root systems are more branched and exhibit an agravitropic phenotype. (bioone.org)

Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. (cancerindex.org)
DRAM2 overexpression also increased proteins CDK4, CyclinD3, and decreased p27 expression, all of which are cell cycle-related factors. (cancerindex.org)
b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)

For the last four years, Fredrik has explored the use of patient-specific cells in cell replacement therapy for Parkinson's disease to identify strategies to safeguard these transplanted cells from disease-related degeneration. (lu.se)
My Ph.D. studies revolved around cell replacement therapy for Parkinson's disease. (lu.se)
My thesis cover illustrates the workflow of generating patient-specific cells for cell replacement therapy for Parkinson's Disease. (lu.se)

Stem cells from human exfoliated deciduous teeth (SHED) are similar to DPSCs in the sense that they are both derived from the dental pulp, but SHED are derived from baby teeth, whereas DPSCs are derived from adult teeth. (wikipedia.org)
Stem cells exist both in embryos and adult cells. (articlecity.com)
They can divide and form all types of differentiated adult cells in the body (neurones, cardiac cells, skin cells, liver cells, etc. (eurekalert.org)
Since 2007, a handful of research teams across the world have been capable of reprogramming human adult cells into induced pluripotent cells (iPSC), which have similar characteristics and potential to human embryonic stem cells (hESC). (eurekalert.org)
The rejuvenated iPSC cells were again differentiated to adult cells and compared to the original old cells, as well as to those obtained using human embryonic pluripotetent stem cells (hESC). (eurekalert.org)
The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
Adult stem cells come from all over the body, plus umbilical cords and placentas. (freerepublic.com)
We are presented with the illogical argument that since some people prefer adult stem cells for non-scientific reasons, they must therefore have little scientific value. (freerepublic.com)
Yet adult stem cells have actually been used therapeutically in the United States since 1968. (freerepublic.com)
Ironically, some of the very diseases he says embryonic stem cells may conquer have long been treated with adult stem cells. (freerepublic.com)
However, 'it is not known for certain that human embryonic stem cells in vitro can give rise to all the different cell types of the adult body. (freerepublic.com)
Meanwhile, three different labs have found three different adult stem cells that may be transformable to all cell types. (freerepublic.com)
Scientists have already discovered at least 14 different types of adult stem cells. (freerepublic.com)
So when Groopman says adult marrow cells may not be 'fully optimal as treatment for many fatal diseases,' he's ignoring at least 13 other adult stem cells that could be. (freerepublic.com)
Almost 'every other week there's another interesting finding of adult (stem) cells turning into neurons or blood cells or heart muscle cells,' notes molecular biologist Eric Olson at the University of Texas Southwestern Medical Center in Dallas. (freerepublic.com)
Unfortunately, it seems every other week there's also another article in the popular press claiming adult stem cells range from nearly worthless to utterly worthless. (freerepublic.com)
Ironically, the original motivation for the massive disinformation campaign is precisely the relative scientific superiority of adult stem cells. (freerepublic.com)
Stem cells are generally divided into two groups, embryonic stem cells and adult stem cells. (vin.com)
Obtaining adult stem cells also does not raise ethical concerns. (vin.com)
1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)

Embryonic stem cells, which must be extracted from embryos three to five days old (known as blastocysts, which contain only about 150 cells at this point of development). (articlecity.com)
Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (springer.com)
Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
Embryonic stem cells come from pulling apart human embryos, and thus have aroused ethical concerns. (freerepublic.com)
Stem cells can be obtained from the embryos that are not used. (msdmanuals.com)
Because the embryos then lose the ability to grow into a complete human being, the use of stem cells from embryos is controversial. (msdmanuals.com)

The overall aim of this thesis has been to assess the potential of autologous grafting in cell replacement therapy for PD. (lu.se)
The focus was on evaluating the potential of using patient-specific cells (i.e., autologous grafting). (lu.se)

Elucidating the function of the mutated gene, ALMS1 , is critical for the development of specific treatments and may uncover pathways relevant to a range of other disorders including common forms of obesity and type 2 diabetes. (springer.com)
TS-derived cells exhibited altered dendrite morphology consistent with what has been observed with other methods, indicating that this method is viable for examining genetic disorders in brain function and development. (bruker.com)
Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
These cells have been studied for the treatment of periodontitis, bone repair, regeneration of the pulp after necrosis as well as the development of new teeth. (bvsalud.org)
Involvement of the corpus, fundus, and gastric antrum, with progressive development of gastric atrophy as a result of loss of gastric glands and partial replacement of gastric glands by intestinal-type epithelium, or intestinal metaplasia (represented by the blue areas in the diagram) characterize multifocal atrophic gastritis. (medscape.com)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
Development and use of induced stem cells is still considered experimental. (msdmanuals.com)

Rapid propagation technology was established and optimized in vitro for Chrysanthemum cinerariifolium (Trev. (bioone.org)
Our studies also show that these NPs have differential requirements for LIF and ciliary neurotrophic factor (CNTF) and for epidermal growth factor (EGF), fibroblast growth factor (FGF-2) and platelet-derived growth factor (PDGF) for their propagation in vitro. (karger.com)

After SHED injection into Goto-Kakizaki rats, type II diabetes mellitus (T2DM) was ameliorated, suggesting the potential for SHED in T2DM therapies. (wikipedia.org)
Recent studies have also shown that the administration of SHED in mice ameliorated the T cell immune imbalance in allergic rhinitis (AR), suggesting the cells' potential in future AR treatments. (wikipedia.org)
We seek to understand the formation and activation of thermogenic fat cells - cells that convert chemical energy into heat - and their potential to fight obesity. (umich.edu)
Catherine Verfaillie of the University of Minnesota was the first to discover ASCs that appear to have the potential to become all cell types. (freerepublic.com)
The only possible advantage of embryonic stem cells is potential. (freerepublic.com)
Of particular interest is their contribution to Developmental Neurotoxicity (DNT) as well as their potential impact on stem cells. (plos.org)
In the present study, the potential of using exosomes isolated from adipose‑derived stem cells (ADSCs) as a therapeutic tool for reducing chronic inflammation and promoting chondrogenesis was investigated using patient‑derived primary cells. (spandidos-publications.com)
Our method expands the scope of CpG methylation profiling from measuring human chronological and biological age from human samples in years, to accurately and rapidly detecting anti-ageing potential of drugs using human cells in vitro , providing a novel accelerated discovery platform to test sought after geroprotectors. (biorxiv.org)
Our results so far indicate that there is potential for meat quality improvements through manipulation of satellite cell activity, and we are investigating this potential further. (poultryhub.org)
Generation of Pluripotent Stem Cells and their Developmental Potential" by Brock J. Conley, Mark Denham et al. (edu.au)
CRT aims to replace neurons that have degenerated in PD, with donor cells that have the potential to functionally re-integrate into the host circuitry. (lu.se)
Stem cells are undifferentiated cells that have the potential to become one of 200 types of cells in the body, including blood, nerve, muscle, heart, glandular, and skin cells. (msdmanuals.com)

Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)

Whilst LiCl exposure did not significantly alter the proportion of cells expressing markers for stem cells/undifferentiated cells (Oct4, SSEA4), neurons (Neurofilament M), astrocytes (GFAP) or cell cycle phase, the drug caused a 1.4-fold increase in total cell number. (plos.org)
Differentiated cells express typical glial markers and show a marked decrease in their proliferative state. (lu.se)
In this study, we examined the acquisition of memory markers after the initial stimulation of naive T cells. (unboundmedicine.com)
However, individual surface markers exhibited a varied relationship to cell division. (unboundmedicine.com)
Although changes in some markers (L-selectin) occurred independently of cell division, changes in other markers were either strictly related to cell division (CD45RB) or were a prerequisite to cell division (CD4, CD44). (unboundmedicine.com)
By immunostaining for cell type specific markers, I have documented the different stages of retinal differentiation and provided insight into the mechanisms of ipRGC differentiation. (indianactsi.org)

Anatomy65

Organisms19

Diseases3

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes70

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science3

Vivo25

IPSCs12

Proliferation16

Capacity to differentiate2

Progenitor cells17

Populations8

Transplantation17

Tissues13

Stem cell45

Tissue37

Adipocytes3

Flow cytometry2

Regenerative11

Bone marrow6

Ability to self-renew1

Functional9

Partially differentiated1

Progenitors3

Retinal gangl3

High throughput1

Endoderm3

Lineages3

Mesenchymal5

Generate2

Human pluripotent stem2

Pluripotency5

Osteogenic1

Chondrocytes1

Cellular9

Organoids6

Somatic cells1

Differentiation of stem cells5

Oxidative stress2

Endothelial cells4

Biology4

Effector4

Self-renewal2

Clinical8

Genomic1

Fetal2

Pluripotent stem cells de1

Fibroblasts1

Predictive1

Genes3

Marker5

MSCs1

Neuronal4

Phenotype1

Proteins4

Replacement Therapy for Parkinson's Disease3

Adult20

Embryos6

Autologous2

Development10

Propagation2

Potential12

Dendritic Cell1

Markers6