Vitro cytotoxic t cells cell cycle. Medical search. Frequent questions

The major molecular defect in extraprostatic and AI PC is the inability of PC cells to initiate apoptosis in response to a variety of stimuli, including different forms of androgen ablation and cytotoxic agents. (nih.gov)
Most chemotherapeutic cytotoxic agents induce apoptosis in cancer cells by direct and indirect action on the cell cycle. (nih.gov)
Preclinical experiments have shown that HDAC inhibitors can induce apoptosis and cell-cycle arrest, and additionally increase the vulnerability of lymphoma cells to cytotoxic T cell. (medscape.com)
The types of cell death induced by honokiol were primarily autophagy and apoptosis. (nature.com)
In contrast, reducing production of intracellular ROS using N -acetylcysteine, a scavenger of ROS, concurrently suppressed honokiol-induced cellular apoptosis, autophagy, and cell cycle arrest. (nature.com)
Furthermore, pretreatment of osteosarcoma cells with PD98059, an inhibitor of ERK1/2, inhibited honokiol-induced apoptosis and autophagy. (nature.com)
Taken together, our results revealed that honokiol caused G0/G1 phase arrest, induced apoptosis, and autophagy via the ROS/ERK1/2 signaling pathway in human osteosarcoma cells. (nature.com)
In particular, autophagy has been found to closely related to apoptosis, which is a kind of programmed cell death. (nature.com)
In vitro and in vivo anti-tumor activity of CoQ 0 against melanoma cells: inhibition of metastasis and induction of cell-cycle arrest and apoptosis. (oncotarget.com)
This study examined the in vitro or in vivo anti-tumor, apoptosis, and anti-metastasis activities of CoQ 0 (0-20 μM) through inhibition of Wnt/β-catenin signaling pathway. (oncotarget.com)
Histological analysis and western blotting confirmed that CoQ 0 significantly decreased the xenografted tumor progression as demonstrated by induction of apoptosis, suppression of β-catenin, and inhibition of cell cycle-, apoptotic-, and metastatic-regulatory proteins. (oncotarget.com)
The most active ozonide (OZ513) was assessed in a propidium iodide cell cycle flow cytometry analysis which measured cell cycle transit and apoptosis. (nebraska.edu)
The Ao peak on cell-cycle analysis was increased after treatment with OZ513 in a concentration dependent fashion which when coupled with results from western blot analysis which showed an increase in cleaved capase-3 and cleaved PARP supported an increase in apoptosis. (nebraska.edu)
Therefore, we aimed to investigate the effects of oleandrin on cancer cell proliferation , viability and apoptosis in vitro and in vivo . (phoenixbiotechnology.com)
We extracted information about the effects of oleandrin on cell proliferation, cell viability , apoptosis and/or cell cycle arrest in in vitro studies, and the effects on tumor size and volume in animal experimental models. (phoenixbiotechnology.com)
Overall, in vitro studies demonstrated that oleandrin was able to inhibit cell proliferation, decrease cell viability, and induce apoptosis and/or cell cycle arrest. (phoenixbiotechnology.com)
The ability of cells to undergo apoptosis showed a strong correlation between cell viability and the ECGLE extract (R 2 = 0.973) as well as ECGLE-based mouthwash formulation (R2 = 0.897). (japsonline.com)
Second, the rapid proliferation of the abnormal myeloblasts, along with a reduction in their ability to undergo programmed cell death (apoptosis), results in their accumulation in the bone marrow, the blood, and, frequently, the spleen and liver. (medscape.com)
Brassinosteroids cause cell cycle arrest and apoptosis of human breast cancer cells. (canadianpinepollen.com)
In fact, many studies have demonstrated the oleuropein and its derivative have proved pharmacological activity against proliferation of cancer cells and several tumor cell lines by different mechanisms such arrest cell cycle and cause apoptosis in cancerous cells, by modulation of miRNA expression and upregulation and downregulation of several genes. (springer.com)
Aberrant overexpression of GSK-3β has been shown to promote tumor growth and chemotherapy resistance in various solid tumors including colon, ovarian, and pancreatic cancers and glioblastoma through differential effects on the pro-survival nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and c-Myc pathways as well on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and p53-mediated apoptotic mechanisms. (outcomes4me.com)

In vitro and in vivo studies have established that Bcl-2 expression confers an antiapoptotic activity against androgen withdrawal and cytotoxic chemotherapy. (nih.gov)
These encouraging in vitro and in vivo findings have led to numerous Phase I/II clinical trials and the approval of two HDAC inhibitors for the treatment of CTCL. (medscape.com)
1. Patient-Derived In Vitro and In Vivo Models of cancer . (nih.gov)
Despite the recent development of technologies giving detailed images of tumours in vivo , direct or indirect ways to measure how many cells are actually killed by a treatment or are resistant to it are still beyond our reach. (biomedcentral.com)
Other categories of organic chemicals have shown to have this property in vitro or in vivo. (nih.gov)
These in-vitro experiments were followed by an in-vivo experiment in which NOD-scid gamma immunodeficient mice were injected subcutaneously with 1â ×â 10 6 BE (2)-c cells followed by immediate treatment with 50-100 mg/kg/day doses of OZ513 administered IP three times per week out to 23 days after injection of tumor. (nebraska.edu)
Six of 12 ozonides tested were active in-vitro at concentrations achievable in vivo with OZ513 being most active (IC50â =â 0.5 mcg/ml). (nebraska.edu)
Conclusions: The antimalarial ozonide OZ513 has effective in-vitro and in-vivo activity against a pleiotropic drug resistant neuroblastoma cell-line. (nebraska.edu)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)
To circumvent these barriers, lentiviral vector systems based on HIV-1 have recently been developed which are able to deliver and express genes in non-dividing cells both in vitro and in vivo. (eurekaselect.com)
9-ING-41 has significant in vitro and in vivo activity as a single agent and/or in combination with standard cytotoxic chemotherapies in a spectrum of solid tumors and hematological malignancies including bladder, breast, glioblastoma, neuroblastoma, pancreatic, sarcomas, and renal cancers as well as lymphomas. (outcomes4me.com)
This unbiased approach driven by human genetics and robust experimental in vitro systems will be combined with appropriate small animal (mouse) in vivo models to define biological significance of proteins/pathways discovered and to develop preclinical animal models for future pharmacological targeting. (nih.gov)
Although CNC exposures are associated with pulmonary inflammation and damage as well as oxidative stress responses and genotoxicity in vivo, studies evaluating cell transformation or tumorigenic potential of CNC's were not previously conducted. (cdc.gov)
Further studies focusing on determining and understanding molecular mechanisms underlying potential CNC cell transformation events and their likelihood to induce tumorigenic effects in vivo are highly warranted. (cdc.gov)
In vitro and in vivo studies have shown that pemetrexed inhibits growth of mesothelioma and non-small cell lung cancer cell lines, and clinical trials in advanced forms of these cancers have shown improvements in overall survival times in pemetrexed treated subjects. (nih.gov)

Hypermethylation of CpG (phosphodiester bonds connecting cytosine and guanine) islands in the promoter region of tumor suppressor genes can lead to the reduced expression of functional proteins controlling the cell cycle and induce proliferation of malignant lymphocytes. (medscape.com)
While elegant studies have defined mechanistic roles for sex hormones and receptors in innate immune cells, much remains to be learned about the cellular and molecular mechanisms of action of ER, PR, and AR in myeloid cells and innate lymphocytes to promote the initiation and resolution of antiviral immunity in the lung. (frontiersin.org)
Materials and Methods: Lymphocytes from six donors were exposed to 2.3 GHz, 10 W/m 2 continuous waves, or 2.3 GHz, 10 W/m 2 pulsed waves (200 Hz pulse frequency, 50% duty cycle). (iiarjournals.org)
In order to enhance the likelihood of discovering a possible effect, we chose to use lymphocytes from several donors, to expose the cells during the whole in vitro period and in one experimental setup to inhibit DNA synthesis and repair in the G 2 -phase of the cell cycle. (iiarjournals.org)
Natural killer (NK) cells are a subset of lymphocytes with a distinct morphology and the ability to directly kill certain target cells via one or more cytolytic mechanisms. (oncotarget.com)
the TB2 tube contains an additional set of peptides targeted to the induction of CMI responses from CD8+ cytotoxic T lymphocytes. (cdc.gov)

Cytotoxic chemotherapy may be used as palliative therapy in AI PC but has not been found effective. (nih.gov)
HMAs can also sensitize lymphoma cells to the effects of chemotherapy by hypomethylating the promoter regions of the TGF-β proteins that regulate cell growth. (medscape.com)
The model was applied to a database of the time course of volumes of breast cancer in patients undergoing pre-operative chemotherapy, for which the initial estimate of proliferating cells by the measure of the percentage of Ki67-positive cells was available. (biomedcentral.com)
Results: It was confirmed that five commonly used chemotherapy drugs had no cytotoxic activity in BE (2)-c cells. (nebraska.edu)
Cancer still remains the number one killer in the Western nations and most treatments for the disease rely on the use of chemotherapy that utilizes drugs that are also toxic against normal healthy cells. (sciepub.com)

Since long-term correction of genetic defects in hematopoietic cells often requires transduction of hematopoietic stem cells, an effective gene transfer into stem cells with efficient long-term and multi-lineage transgene expression is the desired goal for these therapeutic strategies. (eurekaselect.com)

In vitro, melflufen is 50-fold more potent in myeloma cells than the alkylator payload itself due to the increased intracellular alkylator concentration. (cision.com)
We showed a potent in vitro cytotoxic response of a panel of primary ALL to JQ1, independent of their prognostic features but dependent on high MYC expression and coupled with transcriptional downregulation of multiple pro-survival pathways. (birmingham.ac.uk)
OPV to prevent the risk for vaccine-associated paralytic have been reported as potent inhibitors of the replication of picornaviruses in vitro ( 8 ). (cdc.gov)
The leaf extracts and its derivative compounds have shown potent anticancer effects against different cell lines in different organs. (springer.com)
The Actuate 1801 Phase 1/2 study is designed to evaluate the safety and efficacy of 9-ING-41, a potent GSK-3β inhibitor, as a single agent and in combination with cytotoxic agents, in patients with refractory cancers. (outcomes4me.com)

There was a concentration dependent decline in the MYCN and a cyclinD1 protein indicative of anti-proliferative activity and cell cycle disruption. (nebraska.edu)

Several recent in vitro studies on genotoxic effects of RFR have been published. (iiarjournals.org)

Different concentrations of particle suspension are added onto an in vitro culture of RAW264.7 mouse macrophages for a total exposure time of 72 hr, along with untreated control cells. (cdc.gov)
No correlation was found between GHS concentrations and formaldehyde-induced cell toxicity, revealing that the homologous recombination pathway rather than the NHEJ pathway is involved in repair of DNA-protein crosslinks (DPC). (europa.eu)
The two most sensitive DT40 mutants (FANCD1 (BRCA2) and FANC2 -deficient cells) show sensitivity to formaldehyde at concentrations between 10-15 µmol/L, which lay below or in the very low range of those of endogenous formaldehyde. (europa.eu)
Therefore, RKO cells and their isogenic cells disrupted in FANCC or FANCG were exposed to formaldehyde and either FANCC or FANCG were hypersensitive to formaldehyde at concentrations of >20 µmol/L or >38 µmol/L, respectively. (europa.eu)
Notably, non- or sub-cytotoxic concentrations of CoQ 0 markedly inhibited migration and invasion, accompanied by the down-regulation of MMP-2 and -9, and up-regulation of TIMP-1 and -2 expressions in highly metastatic B16F10 cells. (oncotarget.com)
Max Planck Institute (MPI) cells, a non-transformed mouse line that has an AM phenotype and genotype, were treated with mass-, surface area- (s.a.), and particle number- (p.n.) equivalent concentrations of respirable asbestiform and non-asbestiform riebeckite/tremolite EMP for 24h. (cdc.gov)

In order to generate conditions to induce primary T cell responses against different HLA-A+0201-restricted melanoma-associated peptides and to allow further expansion of peptide-specific T cells for adoptive transfer, CD8+ purified T cells from healthy donors and melanoma patients were stimulated in vitro with autologous dendritic cells (DC) pulsed either with the natural (EAAGIGILTV) or modified (ELAO1G1LTV) Melan-A (aa26-36) peptide. (ox.ac.uk)
The risk scores correlated positively with the infiltration features of naive B cells, CD8+ T cells, atpdelnd mast cells, while they correlated negatively with those of M0 macrophages and dendritic cells. (frontiersin.org)

[ 87 ] While initially explored mainly in T-cell NHL, significant activity of HDACi is also observed in B-cell lymphomas. (medscape.com)
Given the close connection between polyamines and cancer, the regulation of polyamine metabolic pathways has attracted attention regarding the mechanisms of action of chemical drugs used to prevent CRC, as the drug most widely used for treating type 2 diabetes (T2D), metformin (Met) exhibits antitumor activity against a variety of cancer cells, with a vaguely defined mechanism. (nih.gov)
Melflufen displays cytotoxic activity against myeloma cell lines resistant to other treatments, including alkylators, and has also demonstrated inhibition of DNA repair induction and angiogenesis in preclinical studies. (cision.com)
Furthermore, GA-D markedly inhibited the generation of reactive oxygen species (ROS) and the expression of p21 and p16 proteins, relieved the cell cycle arrest, and enhanced telomerase activity in senescent hAMSCs. (hindawi.com)
Immune cells express receptors for estrogens (ERα and ERβ), androgens (AR), and progesterone (PR), and experimental manipulation of sex hormone levels or receptors has revealed that sex hormone receptor activity often underlies sex differences in immune cell numbers and/or functional responses in the respiratory tract. (frontiersin.org)
The pink shaded area indicates cells and pathways reported to be elevated in females and/or upon estrogen/ER activity. (frontiersin.org)
The blue shaded area indicates cells and pathways reported to be increased in males and/or upon androgen/AR activity. (frontiersin.org)
Background: Evaluate the anti-tumor activity of ozonide antimalarials using a chemoresistant neuroblastoma cell line, BE (2)-c. (nebraska.edu)
Methods: The activity of 12 ozonides, artemisinin, and two semisynthetic artemisinins were tested for activity against two neuroblastoma cell-lines (BE (2)-c and IMR-32) and the Ewing's Sarcoma cell line A673 in an MTT viability assay. (nebraska.edu)
OZ513 activity was confirmed in IMR-32 and A673 cells. (nebraska.edu)
An increase in the cytoplasmic IAA concentration by pre-loading of IAA into yeast cells abolished the IAA uptake activity by ABCB21 as well as ABCB4. (proteasomesignaling.com)
In-vitro antioxidant activity of bark extracts of Rhizophora mucronata , science, technology and arts research journal, stem bark 95% ethanol, water by cold extraction methods using antioxidant activity 8 . (ijpsr.com)
however, the mechanism of its cytotoxic activity is not well understood. (biomedcentral.com)
Reduction of endogenous mitochondrial TRX2 levels by the cationic triphenylmethane gentian violet (GV) promoted modification of PRX3 by TS and significantly enhanced its cytotoxic activity. (cdc.gov)
Both the extract and formulation were evaluated for antibacterial and in vitro cytotoxic activity in order to determine their potential medicinal value in the oral cavity. (japsonline.com)
The cytotoxic activity was evaluated against human dental pulp primary cells (hDPPC) by calorimetric assay using 3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide. (japsonline.com)
Results obtained demonstrated that A. cadamba bark methanol extract shown significant antiproliferative activity (IC 50 =319±4.98 μg/ml) against human cervical cancer cells as compared with standard cisplatin (IC 50 =5.6±0.52 μg/ml) drug. (ijpsonline.com)
It has been reported that Forest bathing/Shinrin-yoku has the following beneficial effects on human health: 1 Shinrin-yoku increases human natural killer (NK) activity, the number of NK cells, and the intracellular levels of anti-cancer proteins, suggesting a preventive effect on cancers. (researchgate.net)
The resulting IFN-gammaand IL-4-secreting CTL lines demonstrated a significantly higher cytotoxic activity against Melan-A expressing target cells than unpurified CTL. (ox.ac.uk)
Likely the most exciting recent development in their work is the observation that intracellular C3 and C5 are unexpected critical orchestrators of basic cellular processes, including the control of cell metabolism (induction of nutrient flux, glycolysis and oxidative phosphorylation), mitochondrial activity (directionality of the electron transport chain (ETC), impact on TCA cycle and ROS production) and gene transcription. (nih.gov)

16. Indolo-pyrido-isoquinolin based alkaloid inhibits growth, invasion and migration of breast cancer cells via activation of p53-miR34a axis. (nih.gov)
Thiostrepton (TS) is a thiazole antibiotic that inhibits expression of FOXM1, an oncogenic transcription factor required for cell cycle progression and resistance to oncogene-induced oxidative stress. (cdc.gov)
CBGA was also studied in vitro and significantly inhibits the enzyme aldose reductase , a major contributor to the oxidative stress that leads to heart and other problems. (cannabiscompany.com.au)

To date, the use of conventional synthetic chemotherapeutic drugs lack the properties to be considered as effective therapeutic agents, with most of those associated with severe side effects and toxicity of the normal host cells. (sciepub.com)

Sánchez-González C , Ciudad C , Noé V and Izquierdo-Pulido M (2014) Walnut polyphenol metabolites, urolithins A and B, inhibit the expression of the prostate-specific antigen and the androgen receptor in prostate cancer cells , Food Funct. (nih.gov)
When only 1 or a few of antipicornavirus drugs for their ability to inhibit PV rep- compounds had been described for a particular target (for lication in vitro. (cdc.gov)

Petiveria alliacea fraction characterized by de-replication was found to alter actin cytoskeleton organization, induce G2 cell cycle arrest and cause apoptotic cell death in a mitochondria independent way. (biomedcentral.com)
In some cases, expression of the FOS gene has also been associated with apoptotic cell death. (cancerindex.org)
Inhibition of these enzymes leads to decrease in intracellular thymidine and purine which interferes with DNA synthesis and leads to apoptotic cell death in rapidly dividing cells. (nih.gov)

In this study, a senescent human amniotic mesenchymal stem cell (hAMSC) model subjected to oxidative stress was established in vitro using hydrogen peroxide. (hindawi.com)

The therapeutically modified MSCs displayed strong cytotoxicity towards both TMZ-sensitive and TMZ-resistant U251-MG and U87-MG cell lines only in the presence of 5FC. (biomedcentral.com)
The results of this study indicate that Petiveria alliacea exerts multiple biological activities in vitro consistent with cytotoxicity. (biomedcentral.com)

One family of genes involved in cell survival/death control is the Bcl-2 gene family, which consists of homologous proteins that function to regulate distal and crucial commitment steps of the apoptotic pathway. (nih.gov)
Western blots for the apoptotic proteins cleaved capase-3 and cleaved PARP, the highly amplified oncogene MYCN, and the cell cycle regulator CyclinD1, were performed. (nebraska.edu)
We addressed the cytotoxic effect of JQ1, a highly selective inhibitor against the transcriptional regulators, bromodomain and extra-terminal (BET) family of proteins, in paediatric ALL. (birmingham.ac.uk)
Activating NK cells produce anti-cancer proteins, such as perforin and granzyme, in host anti-cancer defense mechanisms, where the targeting of cancer cells is known to be of critical importance [ 2 ]. (oncotarget.com)
As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. (cancerindex.org)
The Laboratory for Complement and Inflammation Research, led by Dr. Claudia Kemper, aims to understand the unexpected roles of complement proteins in the regulation of key basic processes of the cell in health and disease. (nih.gov)
The QuantiFERON®-TB Gold Plus assay is an in vitro diagnostic test using a peptide cocktail simulating ESAT-6 and CFP-10 proteins to stimulate cells in heparinized whole blood. (cdc.gov)
The QuantiFERON®-TB Gold Plus assay tests for Cell Mediated Immune (CMI) responses to peptide antigens that simulate mycobacterial proteins. (cdc.gov)

The balance between cellular proliferation and cell death is regulated by multiple genes or families of genes through the cell cycle. (nih.gov)
The effect of the peptide on cell proliferation and cell death mechanisms was studied by flow cytometry. (mdpi.com)

Cancer is a term to describe with distinguishing characteristics, uncontrolled cell proliferation, invasion of abnormal cells to healthy cells, and their spread towards vital organs via bloodstream 1 . (ijpsr.com)

The plant has an acceptable level of the antioxidant and thrombolytic property along with the moderate level of the cytotoxic property 7 . (ijpsr.com)
Suppression of FOXM1 expression and coincidental activation of ERK1/2 by TS were abrogated by pre-incubation of cells with the antioxidant N-acetyl-L-cysteine (NAC), indicating its mechanism of action in MM cells is redox-dependent. (cdc.gov)

The purpose of the present paper is to investigate the mechanism of action of a pyroglutamate-modified peptide (pE-K092D) on in vitro growth inhibition of MDA-Pca-2b prostate cancer cells. (mdpi.com)
The mechanism of action of TS was investigated in a cell culture model of human MM. As for other tumor cell types, TS inhibited expression of FOXM1 in MM cells in a dose-dependent manner. (cdc.gov)

CoQ 0 exhibits a significant cytotoxic effect on melanoma cell lines (B16F10, B16F1, and A2058), while causing little toxicity toward normal (HaCaT) cells. (oncotarget.com)
1998. Protection by extracellular glutathione against sulfur mustard induced toxicity in vitro. (cdc.gov)
1998. Protection of human upper respiratory tract cell lines against sulphur mustard toxicity by hexamethylenetetramine (HMT). (cdc.gov)
For patients who achieve a CR or a CRi, and/or to allow for recovery from toxicity, the cycle length may be extended up to 28 days (i.e., 7-day treatment-free interval starting on Day 21). (nih.gov)

Furthermore, GA-D upregulated the expression of phosphorylated protein kinase R- (PKR-) like endoplasmic reticulum kinase (PERK), peroxidase III (PRDX3), and nuclear factor-erythroid 2-related factor (NRF2) and promoted intranuclear transfer of NRF2 in senescent cells. (hindawi.com)
It can also affect cell cycle distribution and significantly decrease cyclin D1 expression 10 . (ijpsr.com)
Tumor cell lines were evaluated using the following tests: trypan blue exclusion test, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], flow cytometry, cytoskeleton organization analysis, cell cycle, mitochondria membrane depolarization, clonogenicity test, DNA fragmentation test and differential protein expression by HPLC-Chip/MS analysis. (biomedcentral.com)
It's modes of action include downregulation of NF-κB and decreasing the expression NF-κB target genes including cyclin D1, Bcl-2, anti-apoptotic protein (XIAP) and B-cell lymphoma-extra large (Bcl-XL) leading to inhibition of tumor growth in multiple solid tumor cell and lymphoma lines and patient derived xenograft (PDX) models. (outcomes4me.com)
NF-κB is constitutively active in cancer cells and promotes anti-apoptotic molecule expression. (outcomes4me.com)

Therefore, there is an imperative need to generate innovative therapeutic strategies for cancer treatment, which act by modulating cell proliferation, lowering the risk of malignancy, promoting cytostatic effects and blocking or delaying the progression of tumorigenesis [ 4 ]. (ijpsonline.com)

Peptidases play a key role in protein homeostasis and feature in cellular processes such as cell-cycle progression and programmed cell death. (cision.com)
We designed a simple model of tumour progression during treatment, based on descriptions of the key phenomena of proliferation, quiescence, cell killing and resistance, and giving as output the macroscopically measurable tumour volume and growth fraction. (biomedcentral.com)
Here we show that BET inhibition also reduced c-Myc protein stability and suppressed progression of DNA replication forks in ALL cells. (birmingham.ac.uk)
However, gene transfer strategies with retroviral vectors unable to integrate into non-cycling cells are limited by the quiescent state of the stem cells that have to be stimulated by cytokines to induce cell cycle progression. (eurekaselect.com)
GSK-3β is particularly important in tumor progression and modulation of oncogenes (including beta-catenin, cyclin D1 and c-Myc), cell cycle regulators (e.g. p27Kip1) and mediators of epithelial-mesenchymal transition (e.g. zinc finger protein SNAI1, Snail). (outcomes4me.com)

Further, they have discovered that activation of the key complement components C3 and C5 is not confined to the extracellular space but occurs intracellulary (the 'Complosome') and that intracellular C3 and C5 activation fundamentally dictates the magnitude of Th1-mediated inflammation, the production of granzyme B and IFN- g by cytotoxic CD8+ T cells and the secretion of highly proinflammatory IL-1 b by monocytes and macrophages. (nih.gov)
Intracellular complement activations sustains T cell homeostasis and mediates effector function. (nih.gov)
Short-term exposure to CNC or TF induced intracellular ROS increase and DNA damage while long-term exposure resulted in neoplastic-like transformation demonstrated by increased cell proliferation, anchorage-independent growth, migration and invasion. (cdc.gov)

First, the production of normal blood cells markedly decreases, which results in varying degrees of anemia, thrombocytopenia, and neutropenia. (medscape.com)

We assessed multiple in vitro biological activities of an ethyl acetate soluble plant fraction over several tumor cell lines. (biomedcentral.com)

This technique is adapted for the detection of structural chromosomal aberrations (CAs), such as chromatid-type breaks, chromatid-type exchanges, acentric fragments, dicentric and ring chromosomes, double minutes, endoreduplication, and Robertsonian translocations in vitro after exposure to PM. It is a powerful method to associate a well-established cytogenetic endpoint to epigenetic alterations. (cdc.gov)

Interestingly, transplantation of mesodermal-derived stem cells into aging mice can prolong their life span [ 14 ], while senescent bone marrow MSCs cannot reach the injury site and lose their protection to the lungs due to weak activation, migration, and anti-immune function [ 15 ]. (hindawi.com)
Sex differences in the incidence and severity of respiratory virus infection are widely documented in humans and murine models and correlate with sex biases in numbers and/or functional responses of innate immune cells in homeostasis and lung infection. (frontiersin.org)
Here, we review the literature on sex differences and sex hormone regulation in innate immune cells in the lung in homeostasis and upon respiratory virus infection. (frontiersin.org)
Here, we review literature reports on the sex differences in numbers and functional responses of innate immune cells in the lung and their regulation by sex hormones in homeostasis and during viral lung infection. (frontiersin.org)
Here, we summarize reports of sex differences or sex hormone receptor regulation of innate immune cells. (frontiersin.org)
A balanced type 1 immune response involving different innate immune cells is required early post-infection in the lung for viral clearance. (frontiersin.org)
At later stages of infection, regulatory immune responses mediated by alveolar macrophages and innate lymphoid cells are important for the repair of damaged tissues and renewal of barrier integrity. (frontiersin.org)
Sex differences in numbers, functional responses, plasticity, and survival of innate immune cells regulate the proinflammatory/effector and regulatory/repair phases of infection. (frontiersin.org)
Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
Gene transfer into hematopoietic cells is currently being used to modulate immune responses, to protect hematopoietic cells against cytotoxic drugs or viral genes, and to restore gene deficiencies due to either inborn genetic defects or acquired loss of regular gene function. (eurekaselect.com)
Inflammation-manipulated immune cell plasticity within the tumor microenvironment. (mdpi.com)
The differences in immune cell distributions between survival periods were similar across the breast cancer datasets. (frontiersin.org)
The released N-terminal domain perforates the cell membrane and leads to characteristic morphological changes associated with pyroptosis, including cytoplasmic swelling, membrane rupture, and release of inflammatory factors into the extracellular environment, thus directly amplifying the systemic immune responses ( 9 ). (frontiersin.org)
This study suggests that exposure to forest environments might enhance the immune response of NK cells and activating NK cells in humans. (oncotarget.com)
They are important in the human endocrine and immune systems to induce tumor or virus-infected targeted cell death [ 1 , 2 ]. (oncotarget.com)
They utilize immune/tissue cells from healthy donors, from patients with complement deficiencies, from patients with immune cell-driven infectious, autoimmune disease or cancer and from patients with deviations in novel 'complosome'-regulated pathways for gene and scRNA arrays, epigenetic landscape evaluation, and (spatial) proteomic and metabolomic assessments. (nih.gov)

GA-D significantly inhibited β -galactosidase (a senescence-associated marker) formation, in a dose-dependent manner, with doses ranging from 0.1 μ M to 10 μ M, without inducing cytotoxic side-effects. (hindawi.com)
Metabolic effects of OZ513 in BE (2)-c cells was evaluated. (nebraska.edu)
Oleandrin, as a cytotoxic agent , demonstrated antitumor effects in different types of cancers, however important clinical limitations remain a concern. (phoenixbiotechnology.com)
The protective effects of Masson pine pollen aqueous extract on CCl4-induced oxidative damage of human hepatic cells. (canadianpinepollen.com)
Researchers looked at the cytotoxic effects of CBGA extracted from cannabis. (cannabiscompany.com.au)
We recruited 90 forest staff members in Xitou, Taiwan and 110 urban staff members in Taipei to investigate the health effects of forest environment exposure on NK cells (CD3 − /CD56 + ) and activating NK cells (CD3 − /CD56 + /CD69 + ) in humans. (oncotarget.com)
We also invited 11 middle-aged volunteers in a pilot study to participate in a five-day/four-night forest trip to Xitou forest to investigate the health effects of a forest trip on NK cells and activating NK cells. (oncotarget.com)
This suggests that CNC may have the ability to influence neoplastic-like transformation events in pulmonary epithelial cells and that such effects are dependent on the type/form of CNC. (cdc.gov)

Upstream and downstream components of the FA DNA-repair pathway were tested using mutant chicken DT40 cell lines. (europa.eu)
Mutations in all components of the FA pathway tested sensitized cells to formaldehyde. (europa.eu)
Asgharzade S, Sheikhshabani SH, Ghasempour E, Heidari R, Rahmati S, Mohammadi M, Jazaeri A, Amini-Farsani Z (2020) The effect of oleuropein on apoptotic pathway regulators in breast cancer cells. (springer.com)
GSK-3β helps maintain malignant cell survival and proliferation, particularly in terms of mediating resistance to standard anti-cancer therapies, through the NF-κB pathway. (outcomes4me.com)

For instance Skipper adopted a model of exponential growth, defined by the number of tumour cells at the start of the observation period and by the doubling time [ 14 ]. (biomedcentral.com)
The effect of a cytotoxic drug was described by the fraction of cells killed at each administration, so the complete model (tumour growth + treatment) had only three parameters. (biomedcentral.com)
In that work, considering a single population of tumour cells growing in vitro in the best environmental conditions, a less rich model was unable to fit all available data (a dataset including measures with different techniques). (biomedcentral.com)
Mesenchymal stem cells (MSCs) serve as an attractive vehicle for cell-directed enzyme prodrug therapy (CDEPT) due to their unique tumour-nesting ability. (biomedcentral.com)
Thus, tumour targeting strategies to achieve high local concentration of cytotoxic agent are critical to improve clinical outcome for TMZ-resistant GBM. (biomedcentral.com)

Melflufen (INN melphalan flufenamide) is a first-in-class anti-cancer peptide-drug conjugate that rapidly delivers an alkylating payload into tumor cells. (cision.com)
The company is focusing on the development of the lead product candidate melflufen, a first-in-class anti-cancer peptide-drug conjugate that rapidly delivers an alkylating payload into tumor cells. (cision.com)
T-cell bispecific antibodies (TCB) are novel antibody constructs capable of binding simultaneously to T cells and tumor cells, allowing cross-linking and the formation of immunologic synapses. (aacrjournals.org)
Inherent or acquired resistance can occur simultaneously to multiple drugs in the majority of tumor cells [ 1 - 4 ]. (biomedcentral.com)

Aggressive and fatal cases of hepatosplenic T-cell lymphoma have occurred. (nih.gov)
[ 86 ] Romidepsin (FK288), the other HDACi approved by the FDA for use in CTCL, demonstrated an ORR greater than 30%, with a median duration of response of 13.7 months in patients with r/r peripheral T-cell lymphoma (PTCL) or CTCL. (medscape.com)

Among others Oleuropein and its derivative compounds (hydroxytyrosol, tyrosol and others) were studied in a several types of cancer cells. (springer.com)

4. breast cancer Cell Detection and Characterization from breast Milk-Derived Cells. (nih.gov)
11. Estrogen receptor α dependent regulation of estrogen related receptor β and its role in cell cycle in breast cancer . (nih.gov)
17. Cytotoxic Impact of Costunolide Isolated from Costus speciosus on breast cancer via Differential Regulation of Cell Cycle-An In-vitro and In-silico Approach. (nih.gov)
Assessment of DNA damage response to plasma levels of formaldehyde in chicken DT40 and colorectal cancer (RKO) cells with targeted mutations in various DNA repair genes. (europa.eu)
Various studies have demonstrated that aging accelerates the initiation and/or clonal dominance of mutant stem cells in aging tissues, such as the intestinal epithelium, the hematopoietic system, and the male germline, which shows a causal relationship between the aging-associated accumulation of stem cell mutations and failure of tissue maintenance and cancer suppression [ 2 ]. (hindawi.com)
A Potential Antineoplastic Peptide of Human Prostate Cancer Cells Derived from the Lesser Spotted Dogfish (Scyliorhinus canicula L. (mdpi.com)
Targeted T-cell redirection is a promising field in cancer immunotherapy. (aacrjournals.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Oxidative activation of indole-3-acetic acids to cytotoxic species- a potential new role for plant auxins in cancer therapy. (canadianpinepollen.com)
Cancer is defined as a complex disease characterized by an uncontrolled growth of cells. (ijpsonline.com)
Cancer cells, as well as surrounding stromal and inflammatory cells, engage in well-orchestrated reciprocal interactions to form an inflammatory tumor microenvironment. (mdpi.com)
They found that not only did the CBGA kill colon cancer cells but also arrested the cancer cell cycle. (cannabiscompany.com.au)
While undoubtedly, more research is needed, the researchers were encouraged that CBGA may effectively target not just colon cancer cells but could also prevent the growth and proliferation of polyps. (cannabiscompany.com.au)
Breast cancer is the most common malignancy in women, and there is evidence for the dual role of cell pyroptosis in tumor development. (frontiersin.org)
However, little is known about the relationship between cell pyroptosis and breast cancer and its prognostic value. (frontiersin.org)
We aimed to construct a prognostic model using cell-pyroptosis-related genes to provide innovative insights into the prognosis and treatment of breast cancer. (frontiersin.org)
In conclusion, our findings confirm that cell pyroptosis is closely associated with breast cancer. (frontiersin.org)
Importantly, the prognostic complex values generated from the 10 cell-pyroptosis-related genes based on various clinical features may provide an important basis for future studies on the prognosis of breast cancer. (frontiersin.org)
NF-κB activation is particularly important in cancer cells that have become chemo- and/or radio-resistant. (outcomes4me.com)
In this study, we aimed to assess the neoplastic-like transformation potential of two forms of CNC derived from wood (powder and gel) in human pulmonary epithelial cells (BEAS-2B) in comparison to fibrous tremolite (TF), known to induce lung cancer. (cdc.gov)
Pemetrexed is a parenterally administered folate antagonist and antineoplastic agent, used in the treatment of non-small cell lung cancer and malignant mesothelioma. (nih.gov)
Current indications are for locally advanced or metastatic nonsquamous, non-small cell lung cancer (NSCLC) and for malignant pleural mesothelioma, usually in combination with other antineoplastic agents (such as cisplatin) and with folic acid and vitamin B12 supplementation. (nih.gov)

We screened candidate genes for pyroptosis using public databases and identified 10 cell pyroptosis signature genes with the random forest method. (frontiersin.org)

BESPONSA is a CD22-directed antibody-drug conjugate (ADC) indicated for the treatment of adults with relapsed or refractory B-cell precursor acute lymphoblastic leukemia (ALL). (nih.gov)

Cellular morphology and cytoskeleton integrity of peptide-treated cells were observed by immunofluorescence microscopy. (mdpi.com)

In particular, the percentage of NK cells was significantly higher in the forest group than in the urban group among the subgroups of male, a higher body mass index (≥ 25 kg/m 2 ), without hypertension, lower high-sensitivity C-reactive protein, hyperglycemia, without smoking habit, and with tea drinking habit. (oncotarget.com)

The role of cell pyroptosis in cancers is gradually becoming evident. (frontiersin.org)

Cell viability was determined by the XTT assay. (europa.eu)
At the equal mass, asbestiform EMP were more cytotoxic, however EMP of both habits induced similar LDH leakage and decrease in viability at s.a. and p.n. equivalent doses. (cdc.gov)

Tracking these dynamic events is crucial for understanding fundamental biological mechanisms and providing useful insights for engineering novel cell types and/or devising cellular therapy. (mdpi.com)

At the end of exposure, the culture is treated with colcemid to arrest cells in metaphase. (cdc.gov)
Conclusion: Exposure during the whole cell cycle in inhibited cultures did not resulted in significant differences in chromosomal aberrations as compared to controls. (iiarjournals.org)
A few of them reported that exposure of cultured cells to mobile phone RFR caused DNA damage in some assays ( 4 , 5 ), while other in vitro studies reported no effect on DNA ( 6 - 10 ). (iiarjournals.org)

Additional antigenindependent expansion with anti-CD3/anti-CD28 monoclonal antibodies together with IL-2 gave rise up to 3,000-fold expansion of CD8+ CTL that maintained Melan-A specificity and were able to efficiently lyse Melan-A-expressing melanoma cells. (ox.ac.uk)

Likewise, mesenchymal stem cells (MSCs) are both subject to and key mediators of organismal aging [ 9 ]. (hindawi.com)

Work from Dr. Kemper's lab, however, has highlighted an equally profound impact of complement on adaptive immunity through direct regulation of CD4 + T cells: signals mediated by T cell-expressed anaphylatoxin receptor C3aR and the complement regulator CD46 (which binds the complement activation fragment C3b) are critical checkpoints in human T cell lineage commitment and control initiation and resolution of inflammatory Th1 responses. (nih.gov)

Peroxiredoxin 3 is a redox-dependent target of thiostrepton in malignant mesothelioma cells. (cdc.gov)

When epidemiological evidence is weak, partly due to short follow-up periods, laboratory studies are essential and in vitro studies are a good supplement if the results are replicable and consistent across different methods of testing. (iiarjournals.org)

In the subgroup of patients where killing of cycling cells had the highest score, the drugs showed variable efficacy against quiescent cells. (biomedcentral.com)
The ultimate long-term objective will be to bring new fetal hemoglobin inducers to clinical practice to improve upon drugs such as hydroxyurea, the only FDA-approved drug for treatment of sickle cell disease. (nih.gov)
One is the use of cytotoxic drugs. (nih.gov)
Cytotoxic drugs produce rapid erythroid regeneration following the killing of cycling hematopoietic cells. (nih.gov)
Additionally, the new generation novel drugs should be non-toxic to the host cells and affordable for the patients. (sciepub.com)

In the present study, we investigated the anticancer effect of honokiol (HNK), an active component isolated and purified from the magnolia officinalis on human osteosarcoma cells. (nature.com)
Health effect assessments based on natural killer (NK) cells are an important emerging area of human health. (oncotarget.com)

The urea cycle (UC) removes the excess nitrogen and ammonia generated by nitrogen-containing compound composites or protein breakdown in the human body. (nih.gov)
Thus cells of human origin showed similar hypersensitivity to formaldehyde. (europa.eu)
Earlier results indicating increased sensitivity to formaldehyde in FANCB-deficient cells were validated by testing a human FA gene-deficient cell line (human B cell line NALM-6). (europa.eu)
Our results showed that honokiol caused dose-dependent and time-dependent cell death in human osteosarcoma cells. (nature.com)
Moreover, the ability to overcome chemoresistance in human multiple myeloma cells has been demonstrated 9 . (nature.com)
GFP expressing MSCs was evaluated in cocultures with temozolomide (TMZ)-sensitive or TMZ-resistant human glioblastoma cell lines. (biomedcentral.com)
Proof-of-concept and safety was reported in a first-in-human study (NCT01172964) where a neural stem cell (NSC)-mediated cytosine deaminase/5-fluorocytosine (CD/5FC) prodrug system was tested in patients with recurrent GBM [ 18 ]. (biomedcentral.com)
Incubation of recombinant human PRX3 with TS in vitro also resulted in PRX3 with altered electrophoretic mobility. (cdc.gov)
Abaza L, Talorete TP, Yamada P, Kurita Y, Zarrouk M, Isoda H (2007) Induction of growth inhibition and differentiation of human leukemia HL-60 cells by a Tunisian gerboui olive leaf extract. (springer.com)

The cells also showed enhanced induction of Ser139 phosphorylation of histone H2AX. (europa.eu)
Thus, there is solid clinical, cell biological and biochemical evidence that the induction of fetal hemoglobin can cure SCD and CA, and there is proof of principle that fetal hemoglobin can be induced with pharmacological means, but there are severe limitations to the effectiveness of the currently used fetal hemoglobin inducers. (nih.gov)

C) Retarded-mobility bands corresponding to K48-linked, K63-linked, and linear tetra-ubiquitin chains (red vertical lines) were observed in Phos-tag PAGE only after incubation with mitochondria isolated from CCCP-treated cells. (healthanddietblog.info)

Alveolar macrophages (AM) are the first responders to inhaled particulates, participating in clearance and activating other resident and recruited immunocompetent cells, impacting the long-term outcomes. (cdc.gov)

To assess the impact of PM on nuclear genetic integrity, structural chromosomal aberrations are scored in the metaphase spreads of mouse RAW264.7 macrophage cells. (cdc.gov)

The main manifestation of pyroptosis in cells is swelling that results in cell membrane rupture, which leads to the efflux of contents and activation of an intense inflammatory response ( 10 ). (frontiersin.org)

Cytoplasmic ornithine, the intermediate product of the urea cycle, is a specific substrate for ornithine decarboxylase (ODC, also known as ODC1) for the production of putrescine and is required for tumor growth. (nih.gov)
Reduction of drug efficacy in massive tumours was described by a simple link between the fraction of surviving cells at each treatment and the growth rate. (biomedcentral.com)
Superfoods like wheat-grass juice, germinated broccoli seed, and certain sprouts have these nourishing plant hormones: they help detoxify and have anabolic impact on cell growth. (canadianpinepollen.com)

This in turn results in T-cell activation, expansion, and tumor killing. (aacrjournals.org)
Direct staining of enriched CTL with HLA-A2-Melan-A tetramers revealed a good correlation between the results obtained from the cell surface affinity matrix technology and those obtained from tetrameric complexes. (ox.ac.uk)

Performing a protective function for the cannabis plant, CBGA is produced in the plant's trichomes and acts as a trigger for targeted plant cell necrosis , for natural leaf pruning, to allow the plant to maximise its energy towards its flowers. (cannabiscompany.com.au)
Cell pyroptosis, also known as cellular inflammatory necrosis, is an inflammatory form of programmed cell death (PCD), but it differs considerably from other types of PCD ( 4 ). (frontiersin.org)

Specifically, we highlight ways in which sex differences in innate cells may influence both the proinflammatory/effector phase and the resolution/tissue repair phase important in the host response to respiratory virus infection (Figure 1 ). (frontiersin.org)

After 3-4 weekly stimulation cycles, we were able to generate a strong peptide-specific cytotoxic T lymphocyte (CTL) response in vitro. (ox.ac.uk)
CTL stimulated with the modified Melan-A peptide revealed a frequency of up to 81% CD8+/Melan-A+ T cells measured by MHC tetramer staining. (ox.ac.uk)
Altogether, our study demonstrates that we have developed an efficient method for generating large numbers of peptide-specific T cells in vitro that may be used for adoptive T cell transfer in tumor immunotherapy. (ox.ac.uk)

Our laboratory aims at understanding the unexpected roles of intracellularly active complement in the regulation of key basic processes of the cell in health and disease. (nih.gov)

Anatomy29

Organisms20

Diseases10

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes59

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Health Care2

Apoptosis21

Vivo15

Lymphocytes6

Chemotherapy5

Hematopoietic stem1

Potent5

Anti-proliferative1

Genotoxic1

Concentrations6

Dendritic cells2

Activity20

Inhibits3

Chemotherapeutic1

Inhibit2

Apoptotic cell3

Oxidative stress1

Cytotoxicity2

Proteins8

Proliferation and cell death2

Uncontrolled cell proliferation1

Antioxidant2

Mechanism2

Toxicity4

Expression5

Cytostatic1

Progression5

Intracellular3

Markedly1

Several tumor cell lines1

Endpoint1

Immune16

Effects8

Pathway4

Tumour5

Tumor cells4

Lymphoma2

Compounds1

Cancer22

Genes1

Refractory1

Cytoskeleton1

Significantly higher1

Cancers1

Viability2

Mechanisms1

Exposure3

Melanoma cells1

Mesenchymal stem1

Responses1

Malignant1

Consistent1

Drugs5

Effect2

Human9

Induction2

Lines1

Macrophages1

Genetic1

Membrane1

Growth3

Results2

Necrosis2

Proinflammatory1

Peptide3

Regulation1