Activity lasted for over 30 min at room temperature (24-27 degrees C). The rounding of the in vitro contractile vacuole corresponded to the increased membrane tension of the in vivo contractile vacuole that occurs immediately before fluid expulsion. (biologists.com)
Unlike the in vivo contractile vacuole, the in vitro contractile vacuole did not expel fluid, since it lacked a mechanism to form a pore. (biologists.com)
The subsequent slackening of the in vitro contractile vacuole corresponded to the fluid-filling phase of the in vivo contractile vacuole that occurs at decreased membrane tension. (biologists.com)
Demonstration of contractility of circumferential actin bundles and its morphogenetic significance in pigmented epithelium in vitro and in vivo. (rupress.org)
By in vivo bioassay, in vitro cloning, and dye exclusion measurements, solid tumor nodules treated in vivo with CB at either 100 or 10 mg/kg showed the same viability and tumorigenicity as did vehicle-treated nodules 4 and 6 days after drug treatment, at which time growth inhibition was still apparent. (syr.edu)
This work on formulation, tolerated doses iji vivo, and localized peritu-moral effects of CB now permits evaluation of systemic antitumor effects of cytochalasin B as a single agent. (syr.edu)
Here we demonstrate that Atf3 mRNA levels increase during mouse chondrocyte differentiation in vitro and in vivo . (biomedcentral.com)
It is applied to substantially all types of cells reproducing by mitosis as in vitro and in vivo and it is easier and faster to perform than the chromosome aberration assay. (turkhijyen.org)
At the same time the test enables estimation of cytotoxicity using frequency of nuclear division index in in vitro or the ratio between polychromatic erythrocytes and normochromatic erythrocytes in in vivo. (turkhijyen.org)
Bu test mitoz bölünme ile oluşan hemen hemen tüm hücre tipleri üzerinde in vitro ve in vivo olarak uygulanabilmektedir ve kromozom anormallikleri testine göre daha kolay ve daha hızlı bir uygulamaya sahiptir. (turkhijyen.org)
MN testi aynı zamanda, in vitro çalışmalarda nükleer bölünme indeksi, in vivo çalışmalarda ise polikromatik eritrositler ile normokromatik eritrositler arasındaki oran kullanılarak sitotoksisitenin tahmin edilmesini de sağlamaktadır. (turkhijyen.org)
Most chemopreventive compounds and their analogs and derivatives are initially of plant origin and inhibit spontaneous and chemical mutagenesis in a variety of in vitro and in vivo test systems (Xifeng et al . (scialert.net)
Emperipolesis is amplified in models of murine inflammation associated with platelet overproduction, contributing to platelet production in vitro and in vivo. (elifesciences.org)
In our previous studies, the anti-diabetic properties of root extract of S. spinosum was validated both in-vitro and in-vivo. (biomedcentral.com)
The application of reproductive biotechnology tools such as estrus synchronization (ES), artificial insemination (AI), embryo transfer (ET) through multiple ovulation and in vivo collection of embryos, in-vitro embryo production (IVEP) and cryopreservation, somatic cell nuclear transfer (SCNT) to produce the desired breed and sex of animal, and other reproductive biotechnologies that enhances reproduction potential are important innovations that can address specific needs and purpose for animal reproduction. (ifory.id)
Assay4
The human lymphocytes micronucleus assay has been extensively used in Europe since the cytochalasin-B method was introduced. (uab.cat)
The general opinion was that more basic research on the micronucleus assay itself is required before considering this technique as an alternative to the chromosomal aberration assay, and that it is a suitable method to detect aneuploidy, mainly in vitro, and specially when coupled with molecular cytogenetic techniques. (uab.cat)
This study on the in vitro micronucleus assay, comprising 11 laboratories using human lymphocytes, was coordinated by an organizing committee supported by the SFTG (the French branch of the European Environmental Mutagen Society). (maastrichtuniversity.nl)
Concentrations were tested up to 8333 μg enzyme concentrate dry matter/mL), a recommended regulatory maximum concentration for the in vitro micronucleus assay. (europa.eu)
Oocytes6
Here enucleated recipient cells are treated with cytochalasin B. Cytochalasin B makes the cytoplasm of the oocytes more fluid and makes it possible to aspirate the nuclear genome of the oocyte within a small vesicle of plasma membrane into a micro-needle. (wikipedia.org)
In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. (techscience.com)
in-vitro -matured oocytes were co-incubated with sperm according to a modified two-step culture system. (e-jarb.org)
For the vitrification, in vitro matured bovine MII oocytes were treated by two-step freezing medium without (control group) or with 5 ug/ml cytochalasin-b (CCB group). (koreascholar.com)
2, in each treatment group the in vitro developmental potential of frozen-thawed bovine oocytes post evaluation using Oosight imaging system and then SCNT was investigated. (koreascholar.com)
The disruption of the levels of steroid hormones Animal Genetics and Breeding of the Ministry of Agriculture, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and caused the mature failure of in vitro cultured oocytes. (sagepub.com)
Maturation6
In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. (techscience.com)
Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. (techscience.com)
In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. (techscience.com)
In addition, Atf3 mRNA levels are increased in response to cytochalasin D treatment, an inducer of chondrocyte maturation. (biomedcentral.com)
In the current study, we investigated the beneficial effects of resveratrol on in vitro porcine oocyte maturation under heat stress (HS). (sagepub.com)
After maturation, in vitro fertilization was carried out using TALP media with a final concentration of spermatozoa 1.5 x 106 spermatozoa/ml. (ifory.id)
Embryos3
Along with polyspermy, the suboptimal quality of IVF-derived pig embryos poses another challenge that needs to be addressed for in vitro production systems. (e-jarb.org)
In vitro produced embryos were cultured in CR1aa medium for 8 days and some of them were frozen. (koreascholar.com)
Among in vitro produced blastocyst embryos at day 8, most healthy embryos were freshly transferred on production day and some frozen embryos were direct transferred on appropriate day. (koreascholar.com)
Lymphocytes5
However, it was capable of quantitating micronuclei produced by irradiation of lymphocytes in vitro. (edu.au)
and for lymphocytes irradiated in vitro there was a linear relationship between dose of radiation and number of induced micronuclei. (edu.au)
Nine coded substances were assessed for their ability to induce micronuclei in human lymphocytes in vitro, mitomycin C being used as a positive control. (maastrichtuniversity.nl)
It is concluded that the clastogenic or aneugenic potential in vitro of the substances tested was correctly identified in this study, but that refining the protocol to take into account factors such as the stages of the cell cycle exposed to the compound, or the duration of recovery would be likely to improve the sensitivity of detection using lymphocytes. (maastrichtuniversity.nl)
In vitro treatment of peripheral blood lymphocytes with TSO induced significant number of micronuclei in all samples. (scialert.net)
OECD1
The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells and has been described in detail in OECD TG 487. (re-place.be)
Inhibitor3
It was found that the light-oriented movement of chloroplasts is reversibly inhibited by cytochalasin B. In 1973 researches found that cytochalasin B is a powerful non-competitive inhibitor of glucose transport. (wikipedia.org)
Within our lab, the in vitro micronucleus test with the actin polymerisation inhibitor cytochalasin B is applied. (re-place.be)
I then used the β-actin polymerization inhibitor, cytochalasin D, to inhibit spinogenesis in the ARH. (escholarship.org)
Embryonic development1
Despite considerable technological advancements, polyspermy remains a significant challenge in in-vitro fertilization (IVF) procedures in pigs, disrupting normal embryonic development. (e-jarb.org)
Inhibits3
Cytochalasin B inhibits glucose transport and platelet aggregation. (wikipedia.org)
The extent of MDI intracellular protein binding was not affected by cytochalasin D, a chemical which binds actin filaments and inhibits active uptake into cells. (cdc.gov)
Dexamethasone inhibits phagocytosis by human trabecular meshwork (eye) cells in vitro, with polystyrene particle uptake reduced from 3.5 beads/cell to 1.5 beads/cell, a 57% reduction [ 3364 ]. (nanomedicine.com)
Situ2
Each pigmented epithelial cell bears circumferential actin bundles at its apical level when the pigmented epithelium is established in eyes in situ or in culture in vitro. (rupress.org)
and (iv) to utilize in vitro and in situ Mauthner neuron preparations for studying rapid transport in the axon. (grantome.com)
Silico1
Chemical Composition and In Vitro and In Silico Antileishmanial Evaluation of the Essential Oil from Croton linearis Jacq. (mdpi.com)
Genotoxicity1
There are various ways of detecting the antigenotoxic potential of these substances from short-term genotoxicity assays in vitro to bioassays in humans. (scialert.net)
Cytokinesis1
In the second method, cytokinesis was blocked using cytochalasin B. Micronuclei were scored in cytokinesis-blocked cells. (edu.au)
Cleavage2
This study aims to determine the ability of in vitro embryo cleavage of Bali cattle 48 hours after the fertilization process based on different ovarian reproductive status. (ifory.id)
Deletions were observed to ensure a diverse population of mutant phage, including mismatches that greatly slowed cleavage in vitro Cas12a cleavage trandate price per pill may outcompete less deleterious mutants. (chemdbsoft.com)
Mitosis1
Cytochalasin B allows for the identification and selective analysis of micronucleus frequency in cells that have completed one mitosis, because such cells are binucleate. (re-place.be)
Platelet1
Platelet aggregometry is a standard clinical technique that measures the in vitro aggregability of platelets in response to agonists by using the amount of light transmitted through platelet-rich plasma (PRP) ( Holmsen, 1987 ). (nature.com)
Actin filaments3
It was found that substoichiometric concentrations of cytochalasin B (CB) strongly inhibit network formation by actin filaments. (wikipedia.org)
Cytochalasin B shortens actin filaments by blocking monomer addition at the fast-growing end of polymers. (wikipedia.org)
It is suggested that the predominant mechanism of cytochalasin B is the inhibition of actin filament polymerization through binding to the fast-growing (barbed) end of F-actin filaments. (wikipedia.org)
Proteins1
One of the major electrophoretic identifiable erythrocyte membrane proteins may be the cytochalasin B binding site of erythrocytes. (wikipedia.org)
Polymerization1
ATP was found to be required for contractile vacuole rounding but inhibitors of actin or tubulin polymerization, such as cytochalasin B and Nocodazole, had no effect on the in vitro contractile vacuole's rounding-slackening cycle. (biologists.com)
Detect2
Consequently, this in vitro method can detect both structural and numerical chromosome aberrations in cells that have undergone cell division during or after exposure to the test chemical. (re-place.be)
With the development of the in vitro cytochalasin-B block method to detect MN frequency in binuclear cells which divided once in culture, its reliability and validity have increased. (turkhijyen.org)
Micronuclei1
Cytochalasin B did not itself produce micronuclei. (edu.au)
Nuclear2
The nuclear-centrosome complex was isolated from interphase Chinese hamster ovary (CHO) cells, and, with exogenous brain tubulin as a source of subunits, the centrosome, while attached to the nucleus, was demonstrated to nucleate microtubule formation in vitro. (rupress.org)
Therefore, the nuclear-centrosome complex was dissociated biologically through use of the cytochalasin B procedure for enucleation of cells. (rupress.org)
Membrane3
In vitro membrane-bound vesicles obtained by 'bisecting' (although the two parts were not always identical in size) an in vitro contractile vacuole established their own independent rounding-slackening cycles. (biologists.com)
An additional rounding phase of the in vitro contractile vacuole could be induced by applying suction to a portion of its membrane with a micropipette when the contractile vacuole was in its slackened phase. (biologists.com)
In vitro, it has been observed to move ERα and the ERα-mGluR1 complex to the membrane. (escholarship.org)
Cells3
Contraction of the glycerinated pigmented epithelial cells was inhibited by N-ethylmaleimide but not by cytochalasin B. ITP and UTP also effected the contraction of the glycerinated cells, but GTP and ADP did not. (rupress.org)
In an in vitro model of Sertoli cell-spermatid binding, cortactin was identified around debris and dead germ cells. (biomedcentral.com)
In the presence of cytochalasin D, cortactin immunostaining was punctate and distributed in a pattern similar to that reported for actin in cells exposed to cytochalasin D. Sertoli cells not exposed to FSH, but activated with beads, did not show cortactin immunostaining around the phagocytized beads at any of the time periods. (biomedcentral.com)
Polar1
Cytochalasin B contains several highly polar keto- and hydroxyl groups and one peripheric lipophilic benzyl unit. (wikipedia.org)
Cultures1
Cultures were exposed to the test substances for a short (early or late) time or for a long time, followed by a short or long recovery period, in the presence of cytochalasin B. Each chemical was evaluated, generally in two laboratories, using three treatment schedules at least twice. (maastrichtuniversity.nl)
Treatment1
A correct IZUMO1 repositioning following in vitro induction of F-actin depolymerization, by cytochalasin D treatment, occurred. (ijbs.com)
Cell2
Cytochalasin B, the name of which comes from the Greek cytos (cell) and chalasis (relaxation), is a cell-permeable mycotoxin. (wikipedia.org)
Cell cycle analysis of in vitro cultured goral (Naemorhedus caudatus) adult skin fibroblasts. (druglib.com)
Presence2
This was also seen when preactivated platelets, which cannot undergo further morphological changes, were used, and when platelets were activated in the presence of shape-change inhibitors such as cytochalasin D and vincristine. (nature.com)
Fluid filling occurred in the in vitro contractile vacuole only when it was isolated together with its radial arms. (biologists.com)
Study4
The aim of this in vitro study was to determine whether fibrinogen concentrate, or a combination of factor XIII and fibrinogen concentrates, could reverse a haemodilution-induced coagulopathy during hypothermia. (biomedcentral.com)
SFTG international collaborative study on in vitro micronucleus test II. (maastrichtuniversity.nl)
Results of the study indicate that ginger may protect against chromosomal damage induced by genotoxicant, as evidenced by this in vitro experimentation. (scialert.net)
The current study examined the role of cortactin relative to the cell's phagocytic function in vitro . (biomedcentral.com)
Activity1
This is accompanied by increased Atf3 promoter activity in cytochalasin D-treated chondrocytes. (biomedcentral.com)
Agent1
These in-vitro investigations suggest that Quercetin, in particular, could be a strong therapeutic agent with greater therapeutic efficacy than Cytochalasin B in the therapy of cancer by inhibiting GLUT4. (ijpsr.com)
Higher1
Quercetin, however, was discovered to have a higher docking score than Cytochalasin B and to have broken no rules of the RO5. (ijpsr.com)