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  • molecular
  • CONCLUSIONS: These findings demonstrate that the NOTCH pathway is involved in determining beta-cell fate in vitro and suggest possible molecular targets for induction of beta-cell redifferentiation following in vitro expansion. (biomedsearch.com)
  • chronic myelogen
  • Indirubin, an indigoid active ingredient of a traditional Chinese recipe, was the first example of a CDK inhibitor used to treat human chronic myelogenous leukemia (CML). (aacrjournals.org)
  • desirable
  • Although considerable efforts are still devoted to these chemical families and their structurally related analogues, the identification of new structural classes of protein serine/threonine kinase inhibitors remains highly desirable. (aacrjournals.org)
  • Suppression of CDK9 using RNAi has desirable therapeutic effects in vitro ( 13 ). (aacrjournals.org)
  • A critical issue for the successful development of CDK inhibitors (and indeed the majority of cytoreductive agents) is, therefore, the relationship between desirable, target-specific effects and the onset of nonspecific adverse events that might negatively influence clinical dose escalation ( 20 ). (aacrjournals.org)
  • mice
  • p. 73 ) induced the MS-like disease experimental autoimmune encephalomyelitis (EAE) in mice, and then treated them with etoposide, a topoisomerase inhibitor used clinically to combat cancer. (jimmunol.org)
  • clinically
  • Due to the global shortage of donor corneas, it is vital to engineer corneal tissue in vitro that could potentially be transplanted clinically. (medsci.org)
  • therapeutic
  • This highly effective, functional approach allowed for rapid benchmarking against known CDK inhibitors with undesirable side effects, such as flavopiridol ( Fig. 1B ), and showed SCH 727965 to be a compound with a significantly superior therapeutic profile. (aacrjournals.org)
  • Roles
  • GSK3, an essential kinase with widespread roles in development, cell survival, and metabolism has been demonstrated to be an essential component of theDrosophilacircadian clock. (jcircadianrhythms.com)
  • proliferate
  • Although HCECs may proliferate in vitro , unfortunately, HCECs do not normally proliferate in vivo [ 12 ] due to arrest at the G1 phase in the cell cycle [ 13 ]. (medsci.org)
  • expression
  • and expression, degradation, and availability of naturally occurring protein inhibitors and subcellular localization of these various regulatory components ( 2 , 5 , 6 ). (aacrjournals.org)
  • Nuclear export of p57kip2 was correlated with promoted myelin expression, higher morphological phenotypes, and enhanced myelination in vitro . (jneurosci.org)
  • review
  • Membrane potential-dependent transport of essential metabolic substrates during the cell cycle and/or volume regulation could also play a role (for review, see Wonderlin and Strobl, 1996 ). (jneurosci.org)
  • concentration
  • Cyclin/CDK complexes were typically diluted to a final concentration of 50 μg/mL in a kinase reaction buffer containing 50 mmol/L Tris-HCl (pH 8.0), 10 mmol/L MgCl 2 , 1 mmol/L DTT, and 0.1 mmol/L sodium orthovanadate. (aacrjournals.org)