Vitro colony forming units assay. Medical search. Frequent questions

To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
The Core will provide free training and cost-recovery-based services for in vitro assays of hematopoietic cells including liquid cultures for differentiation down specific hematopoietic lineages as well as clonogenic assays in semisolid medium. (cceh.io)
Clonogenic assays allow for growth of colony forming cells/units (CFU) and long term culture initiating cells (LTC-IC). (cceh.io)
and (iii) Reg4 significantly increases colorectal cancer and pancreatic cancer cell proliferation and their clonogenic potential in stem cell assays. (wustl.edu)
In vitro functional clonogenic assays for IL-7 responsive BM-derived B-cell progenitors demonstrated an increase (∼50%) in colony numbers in the transgenic BM. (sunderland.ac.uk)

The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
At 10 weeks, Fe2O3-exposed cells displayed significant enhanced proliferation, invasion, soft agar colony formation and colony forming unit ability suggesting a neoplastic-like transformation. (cdc.gov)
MWCNT-exposed cells exhibited increased colony formation ability while nCeO2 was negative in all assays except proliferation. (cdc.gov)
In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. (tno.nl)
The culture system, in which a marrow-derived adherent cell population, established in vitro, exerts a long-term promoting influence on proliferation of haemopoietic cells, is reproduced. (tno.nl)
Mouse bone marrow-derived adherent cell populations promoted proliferation of haemopoietic cells in vitro in a liquid culture system for at least 4 weeks. (tno.nl)
In vitro, MPL515/630 but not MPL515/625 or MPL515/625/630 retained the ability to induce TPO-independent proliferation and increase colony-forming unit megakaryocytes (CFU-Mk). (biomedcentral.com)
Wound-healing assay and colony formation analysis were employed for migration and proliferation evaluation. (jmbfs.org)

Decades of efforts have attempted to differentiate the pluripotent stem cells (PSCs) into truly functional hematopoietic stem cells (HSCs), yet the problems of low differentiation efficiency in vitro and poor hematopoiesis reconstitution in vivo still exist, mainly attributing to the lack of solid, reproduced, or pursued differentiation system. (biomedcentral.com)
In this study, we established an in vitro differentiation system yielding in vivo hematopoietic reconstitution hematopoietic cells from mouse PSCs through a 3D induction system followed by coculture with OP9 stromal cells. (biomedcentral.com)
By day 2 from the time of plating, each CFU-E colony will contain between 8 (minimum) and 64 hemoglobinized cells most of which are in their end-stage of erythroid differentiation. (wikipedia.org)
Likewise, an earlier stage of erythroid differentiation may also yield colonies in Epo-only medium, but these colonies would likely be smaller and/or not hemoglobinized, since the stages before the CFU-E stage (MEP and BFU-E) require other factors (IL-3 etc) and more time for growth that will also delay the terminal differentiation and hemoglobinization. (wikipedia.org)
Recent data from in vitro models demonstrating the essential role of stromal support in hematopoiesis shaped the view that cell-cell interactions in the marrow microenvironment are critical for normal hematopoietic function and differentiation. (nih.gov)

Aging of MSCs (both in vivo and in vitro) can affect not only their replicative potential, but also their properties, like immunomodulation and secretory profile, thus possibly compromising their therapeutic effect. (encyclopedia.pub)

Moreover, morphologic and flow cytometric analyses of the transgenic colonies, but not those derived from normal BM, revealed the presence of granulocyte/macrophage colony forming units expressing the HDAC9 transgene, suggesting a lympho-myeloid lineage switch. (sunderland.ac.uk)

Next, Fe2O3 and MWCNT soft agar colonies were isolated and placed in culture to evaluate persistence and longevity of the transformed phenotype. (cdc.gov)
In vitro assays that permit cloning of tumour cells in soft agar have been improved during the last 5 years. (tno.nl)
We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)
Consequently, this propolis underwent fractionation by chromatographic methods monitored through Agar-diffusion assays with these bacteria, which resulted in the isolation and identification of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C), which showed MIC of 62.5 and 250 µg/mL against B. subtilis and S. aureus, respectively. (scielo.br)
Minimum inhibitory concentrations of antibiotics were determined for the gram-positive bacilli using E-test assay (AB BIODISK, Solna, Sweden) on Columbia agar supplemented with 5% sheep blood. (cdc.gov)

We determined the effect of vapour on oxidative stress-induced, PAFR-dependent pneumococcal adhesion to airway epithelial cells in vitro , and on pneumococcal colonisation in the mouse nasopharynx. (ersjournals.com)
Nicotine-containing and nicotine-free E-cigarette vapour increased pneumococcal adhesion to airway cells in vitro . (ersjournals.com)
For example, we found that cigarette smoke extract, fossil fuel-derived particulate matter and welding fumes, by means of the induction of oxidative stress, upregulate PAFR-dependent pneumococcal adhesion to lower airway cells in vitro [ 8 - 10 ]. (ersjournals.com)
Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization. (bvsalud.org)

The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
The underlying mechanisms involved in genomic stability, apoptosis and oxidative stress-related signaling were further analyzed by Western blotting, flow cytometry and immunofluorescence assays. (biomedcentral.com)

Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)
The present study aimed to evaluate the in vitro antimicrobial activity of hydroalcoholic and aqueous extracts from leaves of P. guajava on four bacterial and two fungal samples to determine the range of minimum inhibitory concentration of the extracts. (bvsalud.org)

Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Further, we innovatively demonstrated that notch signaling pathway is responsible for in vitro CD201 + hematopoietic cell induction from mouse PSCs. (biomedcentral.com)

and today is the most widely used in vitro assay for HSPCs. (stemcell.com)
The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. (ox.ac.uk)
Microencapsulated osteoblasts under hypoxia conditions had therefore a significant effect on the expansion potential of HSPCs in vitro. (ox.ac.uk)

The researchers used colony-forming unit assays to track the number of bacteria growing in suspension in vitro after blue light treatment. (the-scientist.com)
Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
Here, we describe the identification and initial characterization of ADS024 (formerly ART24), a novel therapeutic bacterium that can kill C. difficile in vitro with limited impact on other commensal bacteria. (dunianusantara.io)
Use of 16S rDNA sequence analysis showed these species to be closely related and to form a distinct line of descent within the lactic acid bacteria ( 3 ). (cdc.gov)
A. rimae , formerly known as L. rimae ( 1 ), forms short, gram-positive, strictly anaerobic, elliptical bacteria with low G+C content ( 4 ). (cdc.gov)

The Hematopoetic Cell Procurement and Processing core can perform up to 4-color flow cytometry staining and analyses, as well as in-vitro colony-forming assays on research specimens. (fredhutch.org)

Este estudo teve como objetivo avaliar a atividade antimicrobiana in vitro de extratos hidroalcoólico e aquoso das folhas de P. guajava em quatro amostras bacterianas e duas fúngicas para determinar o intervalo de concentração inibitória mínima dos extratos. (bvsalud.org)
Para contribuir para o desenvolvimento de produtos antibacterianos obtidos de própolis produzidos por abelhas nativas do Brasil, 29 tipos de própolis coletados de diferentes colméias no Estado de Minas Gerais, Brasil, foram avaliados quanto à atividade in vitro contra Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, e Staphylococcus aureus. (scielo.br)

We aimed to quantify the colony forming units of megakaryocytes (CFU-Mk) obtained from CD34+ bone marrow cells isolated from patients with MDS and from healthy donors that were cultured in vitro in the presence or absence of azacitidine and with or without the sequential addition of eltrombopag to the culture medium. (unicatt.it)

Colony-forming unit (CFU) assays may also be used to assess editing efficiency by plucking and isolating DNA from individual colonies, followed by PCR analysis. (stemcell.com)
Methylcellulose is a semisolid media additive that allows an investigator to stain (with diaminobenzidine reagent for hemoglobin) and then count individual colonies, each arising from a single plated progenitor that is at the CFU-E stage. (wikipedia.org)

Three anaerobic blood specimens, drawn at entrance into the intensive care unit, yielded gram-positive cocci after 24-h incubation of the first bottle and gram-positive bacilli after 48-h incubation of the 2 other bottles. (cdc.gov)

We observed that the colony morphology of aceE -mut was quite different, smaller and smoother on the solid culture medium than the wild-type (WT) strain during the transposon library screening of M. smegmatis . (biomedcentral.com)
This mutant had obvious differences in colony morphology (smaller plaque, edge smooth and round) and defects in biofilm formation in contrast with the wild-type (WT) strains. (biomedcentral.com)
Core staff will teach YCCEH lab members how to perform the colony forming assays, as well as how to recognize the colony type by morphology. (cceh.io)

Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E)". Blood. (wikipedia.org)
Additional training will include isolation of colonies for assay by cytospin and Wright-Giemsa staining. (cceh.io)

Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)

A decrease in serum C-reactive protein (CRP) and bacterial colony forming unit (CFU) confirmed improved bacterial clearance. (nature.com)
Among the samples from native Brazilian bees, only that from Frieseomelitta varia (Lepeletier, 1836) inhibited in vitro bacterial growth. (scielo.br)

Sub-chronic in vitro exposures with cancer hallmark screening using human primary cell models holds promise as a Tier I screening model for ENM-associated tumorigenesis. (cdc.gov)
Relating in vitro to in vivo exposures with physiologically based tissue dosimetry and tissue response models. (cdc.gov)

Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. (tno.nl)

The MTT and LDH assays were performed to evaluate the cytotoxicity effect of the extract and the determination of cell death. (jmbfs.org)

Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
After incubation, cells will be plated for CFU-GM and CFU-Leuk assays to determine survival of committed hematopoietic progenitors and log-kill of AML cells, and optimal conditions for antileukemic effect with stem-cell sparing. (arizona.edu)
Variables to be examined include agent concentrations, incubation duration and temperature, cell density, sequence of treatment, and number of cycles of in vitro treatment. (arizona.edu)
Flow cytometry analysis, RNA-seq, and cell cycle analysis were used to detect the in vitro hematopoietic ability of endothelial protein C receptor (EPCR, CD201) cells generated in our induction system. (biomedcentral.com)
Adherent cells from mouse bone marrow have been shown to promote the in vitro growth of the AVRij-1 tumour cell line. (tno.nl)
It is possible to see a small spectrum of hemoglobinization level and possibly cell size, indicating that some cells in the colony have achieved the end-stage faster than others. (wikipedia.org)
Cell number in a colony is important because pro-erythroblast stage is also Epo-responsive (expresses Epo receptor), but the proliferative capacity of these cells is not as high, thus yielding a colony with fewer than 8 cells. (wikipedia.org)
These collectively form a thick, dense, poorly permeable cell wall, which not only allows mycobacteria to resist the dry environment and harmful chemicals but also allows it to reproduce in the macrophages [ 4 , 6 ]. (biomedcentral.com)
Colony-forming unit assays yielded denser cell colonies in the HA treated samples compared to the controls, yet no difference in the number of colonies was detected, indicating that the number of stem cells remained unchanged. (europa.eu)
With a variety of assays we could show that MSC represent a cell population which can be expanded for therapeutic applications. (biomedcentral.com)
Besides, EA extract significantly inhibited cell migration and colony formation at 250 to 1000 Âµg/ml concentration. (jmbfs.org)
A Cell Counting Kit-8 assay was used to test cell viability. (researchsquare.com)

CFU-E colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organism's demand for oxygen delivery to the tissues or a hematopoietic disorder. (wikipedia.org)
Absorbance assay, crystal violet staining and adherence assay were used to detect the antifungal activity of flavopiridol. (researchsquare.com)

It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
The in vivo hematopoietic reconstitution potential of c-kit + cells derived from the mouse PSCs was evaluated via m-NSG transplantation assay. (biomedcentral.com)

CD34+ bone marrow cells from 6 MDS patients and 3 controls were expanded in vitro and cultured for 3 days with or without azacitidine. (unicatt.it)

Working out the nuances of a treatment regimen in vitro is an important first step towards future clinical translation. (the-scientist.com)
The clinical application of alginate nanoparticles is still limited because of the burst release of encapsulated drugs and the poor size control of the particles formed. (blogspot.com)

2012 ). In vitro , flavopiridol suppresses the interaction between leukocytes and endothelial cells in the model of murine hepatitis, which is a crucial step in the initiation of inflammation (Schmerwitz et al. (researchsquare.com)

Endogenous progenitors in the PTS model differentiate into astrocytes, which help to form the glial scar lining the syrinx. (edu.au)

Cells at the CFU-E stage express some erythropoietin receptor (EpoR), and thus can be induced to terminally differentiate in vitro in 2-3 days in the presence of only erythropoietin (Epo) (together with the basic contents of culture media: FBS, BSA in IMDM). (wikipedia.org)

In vitro models of human colorectal cancer and pancreatic cancer were used to evaluate the results. (wustl.edu)

We are studying the effects and consequences of protein fusion on the transcriptional and transforming activities of these proteins using in vitro and animal models. (stanford.edu)

DNA was extracted from 1 colony by using a QIAamp tissue kit (QIAGEN, Hilden, Germany) as described by the manufacturer. (cdc.gov)

It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)

Overall, the results showed that EA extract could inhibit several stages in glioblastoma carcinogenesis in vitro. (jmbfs.org)

Elemental analysis of vapour was done by mass spectrometry, and oxidative potential of vapour assessed by antioxidant depletion in vitro . (ersjournals.com)

In vitro studies using extracts from various plants showed an inhibitory action of microbial growth 11 . (bvsalud.org)

it is confirmed that the system is critically dependent on horse serum, and on the in vitro age of the. (tno.nl)
CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. (thermofisher.com)

The participants critically discussed the need for a precise definition of cancer stem cells, the requirement for new markers and more rapid and tractable in vitro and in vivo assays, and the need to develop drug screening strategies to selectively target cancer stem cells to generate therapeutics for this subpopulation of cells that could be resistant to classic treatments while possessing potent tumor-forming capacity. (aacrjournals.org)
ABSTRACT We evaluated the utility of 2 methods for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 g cefoxitin disk. (who.int)

DCDD along with direct tube coagulase assay detected only 80.6% of MRSA/MSSA. (who.int)

2001. Evaluation of benzene exposure in children living in Campania (Italy) by urinary trans,trans -muconic acid assay. (cdc.gov)

Notably, in contrast with the WT, which aggregates and forms biofilm, the aceE -mut lost its ability of growing aggregately and biofilm formation, which are two very important features of mycobacteria. (biomedcentral.com)
Guluronic acid residues can form so-called egg-box complexes with calcium ions or some other divalent metal cations leading to gel formation (Figure 1). (blogspot.com)

7 They used in vitro assays and a human wound skin model to assess whether blue light therapy reduces the P. aeruginosa burden and identified the specific intensities and treatment times needed to maximize antibacterial effects. (the-scientist.com)

Together they form a unique fingerprint. (arizona.edu)

However, to the best of our knowledge, no study in the literature has yet investigated the impact of Staphylococcus saprophyticus on sperm quality and male fertility potential during in vitro fertilization (IVF) techniques. (ecerm.org)
Their self-renewing potential was investigated by colony forming cells assays and in vitro organotypic culture models. (who.int)

Colony analysis by cytospin and Wright-Geimsa staining will also be available as a service. (cceh.io)

For the infected in vitro skin wounds-donor skin biopsies seeded with P. aeruginosa -the latter was the only strategy that showed a significant antibacterial effect. (the-scientist.com)

Additional sex combs-like 1 ( ASXL1 ) mutations have been described in all forms of myeloid neoplasms including chronic myelomonocytic leukemia (CMML) and associated with inferior outcomes, yet the molecular pathogenesis of ASXL1 mutations ( ASXL1 -MT) remains poorly understood. (biomedcentral.com)

CONCLUSION: These findings demonstrated that C3G could alleviate IR in vitro and in vivo to increase insulin sensitivity, which may offer a new insight for regulating glucose metabolism during T2DM by using the natural dietary bioactive components. (bvsalud.org)

Conventional hospitals in Egypt to implement sound 1 mL aliquot was used directly for the methods for the identification of S. au- infection prevention and control pro- LAMP assay and DCDD test. (who.int)

To contribute to the development of antibacterial products from propolis produced by native Brazilian bees, twenty-nine samples of propolis collected from hives in the state of Minas Gerais, Brazil, were screened for in vitro activity against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. (scielo.br)

Anatomy7

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes3

Information Science1

Health Care1

Clonogenic5

Proliferation9

Differentiation5

Vivo and in vitro1

Granulocyte-macrophage1

Agar5

Cells in vitro4

Immunofluorescence2

Antimicrobial2

Hematopoietic cell2

HSPCs3

Bacteria5

Analyses1

Atividade2

Megakaryocytes1

Individual colonies2

Specimens1

Morphology3

Isolation2

Phenotypic1

Bacterial2

Exposures2

Pluripotent1

Performed to evaluate1

Cell12

Detect2

Transplantation2

Bone Marro1

Clinical2

Endothelial1

Progenitors1

Differentiate1

Evaluate1

Protein1

Tissue1

Stromal cells1

Results1

Oxidative1

Inhibitory1

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MRSA1

Evaluation1

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Assess1

Unique fingerprint1

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Analysis1

Effect1

Myeloid1

Increase1

Prevention1

Activity1