Vitro cellular assays. Medical search. Frequent questions

The number and inhibitory activity of CD4(+)CD25(+)FoxP3(+) regulatory T cells were analysed by histology, flow cytometry and in vitro mixed lymphocyte cultures. (uni-koeln.de)
There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)

Cell viability and cytotoxicity assays are used usually for screening of drugs and cytotoxicity tests of chemicals. (benthamscience.com)
Relative cell viability was assessed using MTT assays. (wjgnet.com)
3 H]Flunitrazepam and [ 3 H]TBOB binding, [ 3 H]GABA uptake, cell viability, and morphology were assayed. (hindawi.com)
Proliferation and cell viability were assayed by means of the enzymatic cleavage of the tetrazolium salt WST-1 to formazan dye by cellular mitochondrial dehydrogenases. (scirp.org)
Cell viability assay also revealed that the extracts could safely be used in concentrations of 125 µg/mL. (bvsalud.org)

The contract will be within the framework of current activities of the laboratory aimed at valorizing IBEC technologies and involving characterizing in vitro and in vivo the efficacy and safety of small molecules and its dependence with illumination. (ibecbarcelona.eu)

von Kleist M, Metzner P, Marquet R, Schütte C (2012) HIV-1 Polymerase Inhibition by Nucleoside Analogs: Cellular- and Kinetic Parameters of Efficacy, Susceptibility and Resistance Selection. (plos.org)
In humans, daily doses of 3 million units of interferon result in serum levels of 10-20 U/mL, well above that required for in vitro efficacy ( 8 ) . (cdc.gov)
Efficacy of KBU2046 is demonstrated across several different in vitro models and across multiple murine models of human cancer metastasis, which includes decreased metastasis, decreased bone destruction, and prolonged survival. (nature.com)
GNF-5, an analogue of GNF-2 with improved pharmacokinetic properties, when used in combination with the ATP-competitive inhibitors imatinib or nilotinib, suppressed the emergence of resistance mutations in vitro, displayed additive inhibitory activity in biochemical and cellular assays against T315I mutant human Bcr-Abl and displayed in vivo efficacy against this recalcitrant mutant in a murine bone-marrow transplantation model. (rcsb.org)

cartridge antigen detection assay compared to microplate enzyme immunoassay and modified Kinyoun's acid-fast staining technique for detection of Cryptosporidium in fecal specimens. (scienceopen.com)
the cellular immunological response was studied by the in vitro lymph proliferation assay with SLA antigen. (vin.com)
Humoral and cellular immune responses were then determined by ELISA and in vitro antigen restimulation assay. (uni-koeln.de)

Cytokine profiling was performed by enzyme-linked immunosorbent assay (ELISA). (uni-koeln.de)
Molecularly, RNA sequencing detected dysregulated cytokine and growth factor expression in the BM cellular niches of patients with CML. (lu.se)

Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. (scienceopen.com)
TCDD for every step of the mechanism described for 2,3,7,8-TCDD carcinogenesis in humans including receptor binding, gene expression, protein activity changes, cellular replication, oxidative stress, promotion in initiation-promotion studies and complete carcinogenesis in laboratory animals. (who.int)

In contrast, oral ribavirin doses of 2,400 mg daily yield a steady-state serum concentration of 3-4 _g/mL after several days, approximately 12-40-fold less than the in vitro inhibitory concentrations of 200-500 _M (50-125 _g/mL) noted by Jordan et al. (cdc.gov)
This study establishes a new link between actual human internal POP levels and the endocrine active dose in vitro, applying partitioning-controlled dosing from silicone to the H295R steroidogenesis assay: (1) Measured concentrations of POPs in silicone breast implants were taken from a recent study and silicone disks were loaded according to these measurements. (nih.gov)
that is, it needs to be performed in relevant in vitro systems with pharmacologically relevant concentrations of components to yield biologically relevant data. (genengnews.com)

Forty-four hours after treatment, a colorimetric proliferation assay was used to measure viable cells in each treated well according to the protocol of Promega (Madison, WI). (cdc.gov)

Furthermore, the aim was to study the effects of six naturally occurring nonsynonymous single nucleotide variants on OATP2B1 transport function in vitro. (helsinki.fi)

has identified molecular and cellular events potentially contributing to cardiovascular failure modes that may be measurable by assays as part of a translational toxicology pipeline. (nih.gov)
To understand the fundamental connections between particle properties and human and environmental toxicology we study toxicological effects on all levels, from single cells to ecosystems using various in-vitro, ex-vivo, and in-vivo methods. (lu.se)

The vertical axis represents a colorimetric assay of cellular lactic dehydrogenase, which is directly proportional to cell. (cdc.gov)
1998), a mammalian cell cytogenetic assay (Jantunen et al. (europa.eu)
1986), a human cell micronucleus assay (Budinsky et al. (europa.eu)
2013), a human cell gene mutation assay in the TK locus (Budinsky et al. (europa.eu)
A human cell gene mutation assay in the HPRT locus is also available, although experimental methodology is limited (Budinsky et al. (europa.eu)
By coupling efficient chemical synthesis routes to multiple upfront in parallel phenotypic screens, we identify that KBU2046 inhibits cell motility and cell invasion in vitro. (nature.com)
Using a powerful in vitro platform, we have determined that the severity of clinical manifestations associated with mutations in the RAG, DCLRE1C, and LIG4 genes correlates with the residual activity of the mutant protein and with different degrees of perturbation of T- and B-cell repertoire diversity and composition. (nih.gov)
Next, the effects of miR-637 on glioma cell migration and invasion were determined by using the transwell assay. (scirp.org)
Spheroids and organoids are replacing standard cell culture in many traditional cell-based assays. (corning.com)
Furthermore, as part of the Reduce, Refine, Replace approach to in vivo research (described here in the Journal of the American Association for Laboratory Animal Science ), 3D cell culture offers a highly valuable in vitro alternative. (corning.com)
However, to maximize the cost savings and time efficiency, scientists must be aware of the factors that influence whether or not cell imaging returns high quality data, for both multiwell plate assays and for creating a digital model from z-stack layers. (corning.com)
In an attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min with various free radical generators in PC12 cells by using the dichlorofluorescein (DCF) assay, modified for use by a fluorescent microplate reader. (scienceopen.com)
A microplate assay for measuring cell death in C2C12 cells. (scienceopen.com)
In vitro HaCaT cell photoaging model was used to examine the photoprotection effect of DOP. (bvsalud.org)
Hence, it is desirable to start with a biochemical assay followed by a cell-based assay to corroborate results in a physiological context (that is, to provide orthogonal validation). (genengnews.com)
Specifically, we are studying transcriptional control of kidney proximal tubular cell metabolism, how metabolism is altered in AKI/CKD, and the cellular consequences for injury and repair. (academiccareers.com)
We use in vitro and in vivo models, including live cell metabolic assays and transgenic models. (academiccareers.com)
In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
The Sf9 cell line is isolated from Spodoptera frugiperda insect and is one of the standard in vitro tools in a genetic engineering study. (helsinki.fi)
The goal is to develop human cell- and protein-based assays to more efficiently screen drugs and chemicals for their potential to be toxic to the heart or circulatory system. (nih.gov)
Cell migration was evaluated by using the wound-healing assay and by measuring transendothelial migration of cancer cells. (scirp.org)
The role of different fractions of FFMN in the anti-AD process was examined by Morris water maze (MWM), in-vitro cell, and AChE inhibition assay. (bvsalud.org)
Long-term culture initiating cell assay showed that the mesenchymal stem cells from patients with CML displayed an enhanced supporting capacity for normal and CML BM CD34 + CD38 - cells. (lu.se)
This project will delve into innate immune cell function, establishing in vitro and in vivo systems test innate immune cell function in regeneration. (lu.se)
Inspired from developmental processes, human mesenchymal cell lines can be programmed to form cartilage, bone and bone marrow tissues in vitro and in vivo. (lu.se)

have reported inhibition of WNV by a relatively high concentration of ribavirin (200 _M) given after infection of human oligodendroglial cells in vitro ( 5 ) . (cdc.gov)
H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. (frontiersin.org)
Immunosuppression mediated by MICCop was investigated in vivo by daily assessment of clinical signs of paralysis and in in vitro restimulation assays of peripheral immune cells. (uni-koeln.de)
Adhesion assays were performed at various periods of time on cells grown on plastic. (scirp.org)
Moreover, the migration of MDA-MB231/S283E/Tm1 cells in a wound closure assay is reduced compared to parental cells or those expressing the non-phosphorylatable form of Tm1 (S283A). (scirp.org)
You will also employ functional assays investigating host-pathogen interactions in vitro, such as serum bactericidal assay or adherence to eukaryotic cells. (lu.se)

The aim of this Master's thesis was to create and optimize a cellular uptake assay to study the function of OATP2B1. (helsinki.fi)
A cellular uptake assay with estrone-3-sulfate and a fluorescent probe 4', 5'-dibromofluorescein (DBF) as substrates was set up and optimized. (helsinki.fi)
With the assay, OATP2B1-mediated uptake of variants was compared to the transport activity of OATP2B1 wild type. (helsinki.fi)

The three key elements of the toxicity screening strategy are: Physicochemical Characteristics, In Vitro Assays (cellular and non-cellular), and In Vivo Assays. (cdc.gov)

Antibody-mediated cellular cytotoxicity is also impaired. (medscape.com)

However, there is no comprehensive study on the effect of DOP on skin photoaging combined with in vitro and in vivo models, and its specific mechanism is still unclear. (bvsalud.org)

If we are going to come out of the economy we are now in, then we need technology growth," said Jim Hussey, chief executive officer of NanoInk, in Skokie, Ill. NanoInk is a nanotechnology company started in 2001 that developed Dip Pen Nanolithography technology to create products such as protein detection assays and instruments for nanoscale engineering, said Hussey. (vincentcaprio.org)
Non-cellular assessment of nanoparticle durability, protein interactions, complement activation, and pro-oxidant activity is also considered. (cdc.gov)
In this study the characteristics of the OATP1B1 transporter were reviewed and new in vitro research method to study protein functions was developed. (helsinki.fi)

The development and optimisation of an HPLC-based in vitro serum stability assay for a calcitonin gene-related peptide receptor antagonist peptide. (iasp-pain.org)

The factors that need to be considered include: selection of the most appropriate assay technologies, reading modalities, and experimental models (biochemical, cellular, or animal). (genengnews.com)
Biochemical assays are simpler to execute than their cellular counterparts and offer better consistency. (genengnews.com)

In leukocyte adhesion deficiency I, assays of random migration, chemotaxis, phagocytosis, and killing by neutrophils invariably show deficits in these functions. (medscape.com)

These data suggest that archived coccidioidin retains its potency and specificity and that in vitro test of coccidioidal immunity may have utility in the measurement of coccidioidal cellular immunity. (qxmd.com)

Due to the flexibility of the technique, there are diverse modes of spectra acquisition, from classical transmission and transflection, to highthroughput measurements using micro-plates in transmission mode, to fiber optic probes coupled to Attenuated Total Reflection (ATR) detection, enabling in situ analysis, throughout micro-spectroscopy, with spatial resolution, enabling detection of residual analytes and imaging at the sub-cellular level. (benthamscience.com)
Sensitivity of an assay dictates the detection of the target within the dynamic range of the assay with the caveat that the lower limit of detection could vary marginally from batch to batch. (genengnews.com)
Both assay format and detection technology play major roles in sensitivity. (genengnews.com)
The dynamic range of an assay defines both the lower and upper limits of detection. (genengnews.com)

A549 xenografts by inducing apoptosis, as confirmed by TUNEL assay. (hindawi.com)

In vitro methods are available, but they are validated for only active ingredients. (acs.org)
Develop in silico methods to apply quantitative structure activity relationship analyses and in vitro to in vivo extrapolation. (nih.gov)

The main goal of the Immune Deficiency Genetics Section (IDGS) is to characterize the molecular and cellular mechanisms underlying inborn errors of immunity, and combined immunodeficiencies (CID) in particular, with the ultimate objective of improving diagnosis and treatment of these conditions. (nih.gov)
However, further studies on the expression level and cellular localization of OATP2B1 variants are needed to fully characterize the impact of the variants studied. (helsinki.fi)

Fluorescent microplate assay for respiratory burst of PMNs challenged in vitro with orthopedic metals. (scienceopen.com)
Technologically, luminescent and fluorescent modes offer greater sensitivity compared to absorbance assays. (genengnews.com)

Analytical solutions for the model point out the cellular- and kinetic aspects of inhibition. (plos.org)
The in vitro method developed in this study remains in the research team as a tool to investigate the polymorphisms of the SLCO1B1-gene, the inhibition of the transporter and possible drug interactions. (helsinki.fi)

In vitro techniques allow specific biological and mechanistic pathways to be isolated and tested under controlled conditions, in ways that are not feasible in in vivo tests. (cdc.gov)
Collate and curate mechanistic in vitro cardiovascular data from public data sources and peer-reviewed literature, including Tox21 data. (nih.gov)

Fields such as medical diagnostics and cellular biology would benefit greatly from a technology that enables rapid, quantitative and reproducible assays for many targets within a single sample. (illinois.edu)

In order to evaluate the regulation, expression or activity of a gene, it is necessary to transfer the gene or its manipulated form into the in vitro systems. (benthamscience.com)
Vinyl acetate was negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation. (europa.eu)
Vinyl acetate was shown to be negative in bacterial gene mutation assays using Salmonella typhimurium TA98, TA100, TA1535 and TA1537, with and without rat liver S-9 activation. (europa.eu)

described how analyses of in vitro data and geographic estimates of chemical exposure can be used to prioritize potentially cardiotoxic chemicals for further testing. (nih.gov)
In vitro methodology have focused primarily on skin sensitizing chemicals. (cdc.gov)

Our results indicated that terpinen-4-ol elicited a dose-dependent cytotoxic effect, as determined by MTT assay. (hindawi.com)

Methodologically, the study showed that silicone can be used as reference partitioning phase to transfer in vivo exposure in humans (silicone implants) to in vitro assays (partition-controlled dosing). (nih.gov)
In vitro study was categorized into groups G1 (iPS-MSC), G2 (iPS-MSC dOex-mIRs ), G3 (iPS-MSC + H 2 O 2 /100uM), and G4 (iPS-MSC dOex-mIRs + H 2 O 2 /100uM). (researchsquare.com)
Defining the paradigms and predictable end point(s) enables selection of the assay formats and technologies to be used for the study. (genengnews.com)
Considering that PTZ blocks the chloride channel coupled to the GABA A receptor complex, the present study aimed to analyze the possible modulation of GABAergic homeostasis within synaptic clefts in vitro . (hindawi.com)

Disseminated disease may occur in an otherwise healthy individual, but the risk is significantly higher in individuals with altered cellular immunity due to disease (eg, HIV infection, lymphoma), medical treatment (eg, corticosteroid therapy), or pregnancy. (medscape.com)

Though many traditional sensing technologies for biomarker analysis and fundamental biological studies (i.e. enzyme-linked immunosorbent assays, real-time polymerase chain reaction, etc.) rely on single-parameter measurements, it has become increasingly clear that the inherent complexity of many human illnesses and pathways necessitates quantitative and multiparameter analysis of biological samples. (illinois.edu)

Phenotypic cellular assay models more accurately reflect the complex biology of disease, allows for multiparametric measurement of ex vivo or in vivo events, and enables the characterization of intractable target(s). (genengnews.com)

An archived lot of coccidioidin induces specific coccidioidal delayed-type hypersensitivity and correlates with in vitro assays of coccidioidal cellular immune response. (qxmd.com)
No test for assessing cellular immune response in coccidioidomycosis is currently available in the United States. (qxmd.com)

Taseen Gul, Henah Mehraj Balkhi, Ehtishamul Haq , " Evaluation of Cellular Processes by In Vitro Assays ", Bentham Science Publishers (2018). (benthamscience.com)
This detailed molecular understanding of cellular processes and pathways requires the ability to measure multiple analytes in parallel. (illinois.edu)

SAN DIEGO--( BUSINESS WIRE )-- Quidel Corporation (NASDAQ: QDEL) ("Quidel"), a provider of rapid diagnostic testing solutions, cellular-based virology assays and molecular diagnostic systems, and Ortho Clinical Diagnostics Holdings plc ("Ortho"), one of the world's larger pure-play in vitro diagnostics companies, announced today that the closing of the previously announced Ortho transaction is expected to occur on May 27, 2022. (biospace.com)
Ortho Clinical Diagnostics Holdings plc (Nasdaq: OCDX) is one of the world's larger pure-play in vitro diagnostics (IVD) companies dedicated to transforming patient care. (biospace.com)
The relationship of in vitro lymphocyte defects to clinical infections is not understood. (medscape.com)

Apply WGCNA approaches to construct cardiac-relevant modules from both in vivo (rat heart) and in vitro (rat and human cardiomyocyte) model systems. (nih.gov)
Identify modules that exhibit conservation across model systems/species to understand where in vitro systems best model toxicological response. (nih.gov)

As noted by In Vitro Cellular & Developmental Biology - Animal , you may not want to rely on assays developed for 2D when moving into 3D data gathering. (corning.com)

Tyrosine phosphorylated regions in proteins function as specific binding sites for the SH2 domains containing cellular signalling proteins. (lu.se)

Optimization of experimental assay design is crucial for the overall success of any drug discovery initiative. (genengnews.com)

We conclude that interferon alpha-2b possesses greater therapeutic activity in vitro than ribavirin, with a potentially greater therapeutic ratio in humans. (cdc.gov)

Ribavirin was protective but not therapeutic in vitro. (cdc.gov)

The key studies are considered to be bacterial mutation assays (McCann et al. (europa.eu)
Cellular studies of NTD and CTD oligomerization interface mutants demonstrate the importance of each interface in matrix assembly. (uci.edu)

This paradigm may be widely adopted as a robust early-stage platform for screening peptide stability to rule out candidates with low in vitro stability, which would likely translate into poor in vivo pharmacokinetics. (iasp-pain.org)

Using in vitro and cellular techniques, we present a mVP40 assembly model highlighting two distinct oligomerization interfaces: the (N-terminal domain (NTD) and C-terminal domain (CTD)) in mVP40. (uci.edu)

Among them, CXCL14 was lost in the BM cellular niches in contrast to its expression in healthy BM. (lu.se)

Restoring CXCL14 significantly inhibited CML LSC maintenance and enhanced their response to imatinib in vitro, and CML engraftment in vivo in NSG-SGM3 mice. (lu.se)

Before cellular metabolism can occur, a drug must first enter the hepatocyte. (helsinki.fi)

Tier 1 in vivo assays are proposed for pulmonary, oral, skin and injection exposures, and Tier 2 evaluations for pulmonary exposures are also proposed. (cdc.gov)

Anatomy3

Chemicals and Drugs3

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Phenomena and Processes11

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