Vitro cell transformation assay. Medical search. Frequent questions

The mechanism of action of ciclopirox has been investigated using various in vitro and in vivo infection models. (guidelinecentral.com)
Using a combination of simplistic 3D in-vitro assays and live-cell in-vivo imaging we study the control mechanisms of cellular dynamics and motile cell transformations during early development. (irbbarcelona.org)
We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)
Furthermore, we find direct evidence of a role for Id1 with significant suppression of in vitro transformation and in vivo tumorigenesis in COX-2-overexpressing GBM cells where Id1 has been knocked down. (oncotarget.com)
In vitro, GT is studied in suspensions of billions of cells and can be used to assay transfer of traits between strains, such as antibiotic resistance and virulence, but does not reproduce the rapid and extensive of transfer observed in vivo. (uic.edu)
Nevertheless, sufficient information is available when taking in account all in vitro studies collectively and when considering the reliable in vivo micronucleus study. (europa.eu)
In this review, we discuss the various factors of BNNT production that can influence its toxicity followed by summarizing the toxicity findings from in vitro and in vivo studies conducted to date, including a review of particle clearance observed with various exposure routes. (cdc.gov)

Proliferation and cell viability were assayed by means of the enzymatic cleavage of the tetrazolium salt WST-1 to formazan dye by cellular mitochondrial dehydrogenases. (scirp.org)
FLT3 ITD triggers the proliferation of the quiescent hematopoietic stem cell (HSC) pool but fails to directly transform HSCs. (biomedcentral.com)
Moreover, thrombin-treated MSCs maintained the phenotypic features, in vitro osteogenesis and adipogenesis capacities, and inhibitory activity on Phytohemagglutinin-induced allogeneic lymphocyte proliferation. (biomedcentral.com)
The high proliferation rate and plating efficiency of untreated cells are necessary for the appropriate performance of the study. (europa.eu)
Cell cycle length, doubling time or proliferation index: Doubling time 12 to 16 hours in stock cultures. (europa.eu)
Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
CCK-8 assay and flow cytometry were used to assess the cell proliferation and apoptosis abilities, respectively. (bvsalud.org)
Knockdown of CRABP2 promoted the cell apoptosis and inhibited cell proliferation, migration, and invasion in cervical carcinoma cells, whereas CRABP2 overexpression exhibited the opposite results. (bvsalud.org)
Treatment with siITGB1 or a FAK inhibitor PF-562271 or an ERK inhibitor FR180204 reversed the promoting effects of CRABP2 on cell proliferation, migration, and invasion. (bvsalud.org)
Moreover, the overexpression of CRABP2 reverted the HPV16 E6/E7 knockdown-induced inhibition of cell proliferation, migration, and invasion in cervical cancer cells. (bvsalud.org)
CytoSelect MTT Cell Proliferation Assay provides a colorimetric format for measuring and monitoring cell proliferation. (mytaq.com)
Cells can be plated and then treated with compounds or agents that affect proliferation. (mytaq.com)
Cells are then detected with the proliferation reagent, which is converted in live cells from the yellow tetrazole MTT to the purple formazan form by a cellular reductase (Figure 1). (mytaq.com)
An increase in cell proliferation is accompanied by an increased signal, while a decrease in cell proliferation (and signal) can indicate the toxic effects of compounds or suboptimal culture conditions. (mytaq.com)
This cell proliferation reagent can be used to detect proliferation in bacteria, yeast, fungi, protozoa as well as cultured mammalian and piscine cells. (mytaq.com)
The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

In an in vitro mammalian chromosome aberration test, human peripheral blood lymphocytes were exposed to the read across substance (CAS 768 -94 -5, unknown purity), in the presence and absence of metabolic activation (Brambilla and Martelli 2009). (europa.eu)

In a CF-1 mouse bone marrow micronucleus assay, performed according to GLP and similar to OECD 474, seven males per dose were treated intraperitoneal on three consecutive days with the test item (98% a.i.) at doses of 15, 30 or 60 mg/kg bw (Kaefer et al. (europa.eu)

Via flow cytometry in both cell lines a clear transformation of cell cycle induced by treatment with the drugs could be demonstrated, but these effects seem to be rather a single effect of flavopiridol. (uni-marburg.de)
GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
In this study, highly purified, flow-cytometry sorted, classified in relation to normal B-cell differentiation [1]. (lu.se)

Other carcinogens may cause cancer through a variety of mechanisms without producing mutations, such as tumour promotion, immunosuppression that reduces the ability to fight cancer cells or pathogens that can cause cancer, disruption of the endocrine system (e.g. in breast cancer), tissue-specific toxicity, and inflammation (e.g. in colorectal cancer). (wikipedia.org)
Information is available from two studies that have investigated the in vitro bacterial genetic toxicity potential of 1-hexene. (europa.eu)
Information is available from one study that has investigated the in vitro bacterial genetic toxicity potential of Alkenes, C6. (europa.eu)
A toxicity pretest was conducted prior to the main mutagenicity assay to determine the highest dose of the compound that could be used in the main assay. (europa.eu)
The lung toxicity was measured using lactate dehydrogenase (LDH) activity assay, lipid hydroperoxide (LPO) assay, histology, and immunohistology analysis. (news-medical.net)
However, pretreating the mice with GLSF was found to reduce B[a]P-induced lung toxicity (with an efficacy comparable to curcumin), as demonstrated by the improved histology of the harvested lung tissue samples, as well as lower LDH activity, malondialdehyde levels, and inflammatory cell infiltration in them. (news-medical.net)
There are several, not fully reliable in vitro studies available to assess the genetic toxicity. (europa.eu)
The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)

Malignant transformation in vitro was determined by using the anchorage- independent growth assay on soft agar. (scirp.org)
The current study aimed to investigate the role of CRABP2 in the malignant phenotypes of cervical cancer cells. (bvsalud.org)
The malignant tumours consisted of 18 adenoid cystic adenomas, 10 mucoepidermoid carcinomas, 5 acinic cell carcinomas, 5 carcinoma in pleomorphic adenoma as well as of 12 other malignancies belonging to 7 different tumour entities. (researchgate.net)
Finally, GBM cells with COX-2 or Id1 overexpression show greater migration/invasive potential and tumors that arise from these cells also display increased microvessel density, results in line with the increased malignant potential seen in these cells. (oncotarget.com)
PC-ALCL is one of the primary cutaneous CD30 + T-cell lymphoproliferative disorders, a wide spectrum of disease, with lymphomatoid papulosis (LyP) at the benign end of the spectrum and PC-ALCL at the malignant end. (medscape.com)

Any supplementary information relevant to cytotoxicity: Mitotic index was determined in 1000 cells in each replicate. (europa.eu)
For this, the in vitro activity of four non-dihydropyridine agents (amrinone, fendiline, mibefradil, and lidoflazine) was tested against different Leishmania species and their cytotoxicity to mammalian cells was evaluated. (hindawi.com)

In a mammalian cell gene mutation assay (HPRT locus), Chinese Hamster Ovary (CHO) cells cultured in vitro were exposed to the read across substance (CAS 768 -94 -5, unknown purity), in the presence and absence of metabolic activation (Brambilla and Martelli 2009). (europa.eu)

Positive results with TCE in a mammalian cell transformation assay conflict with negative results in the Ames Salmonella assay. (cdc.gov)
Based on these results it was concluded that the 1-hexene was not mutagenic to the five Salmonella strains used in the assay up to and including the highest dose of 320 ug/plate. (europa.eu)
The read across substance was negative in Salmonella typhimurium assay with and without metabolic activation. (europa.eu)

The mutagenicity assay indicated that the test chemical did not exhibit a positive dose related increase in the number of revertants in any of the tester strains used. (europa.eu)
There was no evidence of mutagenicity in the mammalian cell gene mutation assay. (europa.eu)
T he publication is only a secondary source and does not satisfy the requirement for Test Guideline OECD 476 for in vitro mutagenicity (mammalian forward gene mutation) data (limited documentation, no data about investigated concentrations, no data about metabolic activation system given and no data about used positive controls). (europa.eu)

Radioactivity - especially, chromium 51 ( 51 Cr) in the form of Na 2 51 CrO 4 - is an inexpensive way to rapidly and nonspecifically label cellular proteins within the target cells (1). (jove.com)
Upon the loss of membrane integrity of the target cells as a result of the cytotoxic activity of the effector cells, the 51 Cr-labelled cellular proteins within the target cells are released into the culture supernatant, becoming available for quantitation. (jove.com)
This system provides a distinct means to study the mechanism of mammary neoplastic transformation at cellular and molecular levels. (eurekamag.com)
In the in vitro experiments, GLSF was found to inhibit the BPDE-induced transformation of BEAS-2B cells, indicating its potential role in controlling the cellular alterations resulting from the exposure of these bronchial epithelial cells to known carcinogens. (news-medical.net)
Insight into the dynamics of single cellular building blocks is key to our understanding of tissue development, homeostasis and disease: shape change, active motility and collective cell behavior establish the architecture of complex multi-cellular tissue patterns in early morphogenesis and similar processes are activated during tissue homeostasis and pathological conditions. (irbbarcelona.org)
Furthermore, mechanical forces and cellular stress in 3D environments can rapidly change cytoskeletal dynamics and migration competence in single and collectively migrating cells, providing a multi-scale feedback coupling between cellular and tissue-scale dynamics. (irbbarcelona.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The cell-mediated immunity is measured by using a delayed-type hypersensitivity (DTH) skin test ( 5 ) or an in vitro assay of cellular immunity to Coccidioides spp. (cdc.gov)

Requirements for RNA and DNA synthesis in the transformation of mouse embryo cells by murine sarcoma virus (moloney). (microbiologyresearch.org)
Morphological transformation of mouse embryo cells in vitro by murine sarcoma virus (harvey). (microbiologyresearch.org)
In an in vitro mammalian cell transformation assay RLV-Fischer rat embryo cells (RLV/RE) were exposed to the test item (unknown purity), at concentrations of 1 and 2 µg/mL for 9 days (Price et al. (europa.eu)

In particular EF1A2 is overexpressed in several tumor cells and participates in drug resistence processes. (unina.it)
This may be due to late diagnosis of these tumors in our patients as most cases diagnosed at advanced stage and the sections were taken from deep tumor tissue to increase the number of tumor cells in our samples. (researchgate.net)
These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)
ALCL was recognized in 1985, when tumor cells consistently demonstrated labeling by the monoclonal antibody Ki-1, a marker later shown to recognize the CD30 antigen. (medscape.com)
This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

Cell Biolabs Laboratories manufactures the prices of sta-600 cell biolabs reagents distributed by Genprice. (mytaq.com)
The Prices Of Sta-600 Cell Biolabs reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (mytaq.com)
Description: Cell Biolabs? (mytaq.com)

Previous experiments in H1355 cells demonstrated that IFN treatment results in an antiapoptotical answer during which EF1A2 is increasingly expressed due to its RAF kinase mediated phosphorylation. (unina.it)
As this interaction establishes a new link between the mitogenic cascade and protein biosynthesis, in vitro kinase assays were performed in presence of B- and C-RAF kinase and recombinant EF1A1/2 to investigate this important interaction. (unina.it)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)

In the last years, these isoforms were found to be involved in several different processes like senescence, apoptosis and transformation. (unina.it)
Elesclomol induces oxidative stress and triggers mitochondrial-induced apoptosis in cancer cells. (mytaq.com)
Elevated COX-2 is associated with increased angiogenesis, tumor invasion and promotion of tumor cell resistance to apoptosis [ 5 - 8 ]. (oncotarget.com)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)

Moreover, we found that the cells expressing the S283A mutants form more colonies in soft agar that those expressing the S283E mutants. (scirp.org)
On the day of the assay, top agar, tester strains, test or control material and S9 mix or saline were combined and poured into a plate containing a layer of agar. (europa.eu)
A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. (mytaq.com)
GBM cells with COX-2 overexpression show increased growth of colonies in soft agar. (oncotarget.com)

Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. (mytaq.com)
Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. (chipready.com)

PTEN was validated by dual luciferase assay as a target gene of miR-183. (nature.com)

The test item did not induce gene mutations or chromosomal aberrations in the V79 Chinese hamster cell line nor gene mutations in S. typhimurium bacterial cells in the presence and absence of metabolic activation. (europa.eu)
The test substance has a potential for point mutation-inducing activity in a reverse bacterial mutation assay according to an OECD 471 (Ames) assay. (europa.eu)
In a bacterial reverse gene mutation assay, S. typhimurium was exposed to the read across test item (CAS 768 -94 -5, purity unknown) in the presence and absence of mammalian metabolic activation (Brambilla and Martelli 2009). (europa.eu)

Cell migration was evaluated by using the wound-healing assay and by measuring transendothelial migration of cancer cells. (scirp.org)
Moreover, the migration of MDA-MB231/S283E/Tm1 cells in a wound closure assay is reduced compared to parental cells or those expressing the non-phosphorylatable form of Tm1 (S283A). (scirp.org)
Similarly, the transendothelial migration of MDA-MB231/S283E/Tm1 cells is also reduced as compared to the other cell lines. (scirp.org)
Simoneau, B., Houle, F. and Huot, J. (2012) Regulation of Endothelial Permeability and Transendothelial Migration of Cancer Cells by Tropomyosin-1 Phosphorylation. (scirp.org)
Then the survival cells were collected to investigate their potential of colony formation, attachment, invasion, and migration. (scielo.br)
Mechanical forces and tissue crowding are getting into focus as important modulators of cytoskeletal dynamics, migration behavior and cell state. (irbbarcelona.org)
This experimental framework recently allowed us to uncover a contractility-based polarization mechanism that drives the rapid transformation of cells into a fast-amoeboid migration mode. (irbbarcelona.org)
Wound healing assay and transwell assay were employed to measure the cell migration and invasion abilities, respectively. (bvsalud.org)
To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)

Genotoxic carcinogens react directly with the DNA of the cell, finally resulting in a mutation. (invitrojobs.com)
There was no evidence for induction of genome mutation, as determined in the cell transformation assay in the absence of metabolic activation. (europa.eu)

The test substance is considered to cause structural chromosomal aberrations in cultured mammalian cells according to an in vitro OECD 473 chromosomal aberration assay. (europa.eu)

These assays have been used to determine the ability of T cells, NK cells, or any other immune cell to kill target cells in an antigen-specific or -nonspecific manner. (jove.com)
Infection of rabbits was further demonstrated by antigen capture assay of p24 in PBMC and lymph node cultures and PCR amplification of proviral sequences from PBMC. (wustl.edu)
Samples were assayed for the P. falciparum HRP2 antigen and anti-malarial IgG antibodies. (cdc.gov)
Anaplastic large cell lymphomas (ALCLs) are distinguished from other lymphomas by their anaplastic cytology and constant membrane expression of the CD30 antigen (an activation marker for B or T cells). (medscape.com)

The test substance has a potential to induce forward mutations in mammalian cell lines (HGPRT gen locus) according to OECD 476 HGPRT assay. (europa.eu)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)

High-efficiency neoplastic transformation of mouse mammary epithelial cells in primary collagen gel culture was induced by N-methyl-N-nitrosourea (MNU). (eurekamag.com)
Mammary epithelial cells, isolated from virgin BALB/c mice, were embedded within collagen gels and grown in a serum-free medium containing prolactin, progesterone, and linoleic acid. (eurekamag.com)
Other studies in oral cancer [23,24] reported that there is no relationship between the histopathological grade of oral epithelial dysplasia or oral squamous cell carcinoma and the ploidy status. (researchgate.net)
In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)

Development of 3T3-like lines from Balb/c mouse embryo cultures: transformation susceptibility to SV40. (microbiologyresearch.org)

Different cell culture characteristics of two strains of murine sarcoma virus. (microbiologyresearch.org)
HPV infections have received particular attention in recent years, as high-risk strains have been linked to some cases of oral squamous cell carcinoma. (medscape.com)

In vitro susceptibility testing methods for determining ciclopirox MIC values against the dermatophytic molds, including Trichophyton rubrum species, have not been standardized or validated. (guidelinecentral.com)

For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)

Mesenchymal stem cells (MSCs) are adult multipotent cells that were initially isolated from bone marrow[ 1 ] and characterized by the fibroblast-like appearance in culture and the capacities to form bone, adipose and cartilage. (biomedcentral.com)
However, the quantity of MSCs is very low in bone marrow (about 0.001 to 0.01% of the mononuclear cells) and in vitro expansion is the prerequisite for their clinical application. (biomedcentral.com)
2010). Bone marrow cells were harvested on day 4. (europa.eu)

One of the main functions of the cells of the immune system is to remove target cells that have been infected with viruses or have undergone transformation into a tumor cell. (jove.com)
In vitro assays for measuring the cytotoxic capacity of immune cells have been a staple in laboratories for many years. (jove.com)
With the explosion in tumor immunotherapy research in recent years, there is growing interest in finding agents to increase the cytotoxic activity of immune cells to improve patient outcomes. (jove.com)
Conversely, some diseases are hallmarked by the overexuberant activity of immune cell cytotoxic activity, resulting in efforts to identify agents to temper these responses. (jove.com)
As with any assay examining the function of immune cells in vitro , there are a number of important considerations to consider improving the performance of the experiment. (jove.com)
They react i.e. also with proteins or immune cells, as is the case with asbestos. (invitrojobs.com)
Newer strategies recognize the close biologic interactions between the skeletal cells and the immune cells. (medscape.com)
At 7wk, HF-fed animals exhibited several immune alterations (blood leukocyte/neutrophil number, lymph node B-cell proportionality)-effects which were more pronounced in SD rats. (cdc.gov)

A single exposure or multiple exposures of these cells to MNU was effective in inducing tumorigenic cells that produced palpable tumors as early as 6 weeks after transplantation. (eurekamag.com)
Two treatments with MNU (100 micrograms/ml) were optimal for neoplastic transformation and produced tumors in 79% of the injected fat pads. (eurekamag.com)
Genome-scale mutational signatures of aflatoxin in cells, mice, and human tumors. (who.int)
Chronic exposure of rats resulted in increased thyroid follicular cell tumors from sustained perturbation of thyroid hormone homeostasis. (cdc.gov)
B-cell lymphomas (BCLs) constitute a diverse set of tially identify new functional, diagnostic, and therapeutic tumors, both morphologically and clinically, that are mainly targets. (lu.se)

Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. (mytaq.com)

Suitability of cells: The V79 cell line has been used for many years in in vitro experiments with success. (europa.eu)

Each kit provides sufficient reagents to perform up to a total of 96 assays and can detect HIF-1 Alpha from human, mouse, or rat. (mytaq.com)

These in vitro assays involve the mixing of different cell populations, as well as using a relatively low number of both effector and target cells. (jove.com)
Finally, data analysis is dependent on the inclusion of positive and negative control target cell populations. (jove.com)
Eleven to 14 days after the final carcinogen treatment, the cells were removed from the collagen gels and injected into the cleared mammary fat pads of syngeneic hosts to assay for transformed cell populations. (eurekamag.com)
In three cases which initially were taken for pleomorphic adenomas by routine histological examination, aneuploid cell populations exposed by DNA flow cytometric analysis gave rise to a closer inspection of the suspect lesions. (researchgate.net)

To determine the impacts of miR-183 on cell viability, the MTT assay was used. (nature.com)

Background: Tropomyosin 1 alpha chain (Tm1) is an actin-binding protein that regulates the endothelial cell response to oxidative stress following its phosphorylation at Serine 283 (S283). (scirp.org)
Families of related leukemia fusion proteins that share genomic and biological properties represent unique opportunities to study how the combination of distinct functional protein modules can drive oncogenic transformation. (nature.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

Collagen-based Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators. (mytaq.com)

Lonafarnib inhibits the farnesylation of H-Ras and the attachment of Ras to the cell membrane. (uni-marburg.de)

During serial passage of mouse embryo fibroblasts in vitro it was found that an established line of cells was more susceptible to murine sarcoma virus transformation than early passages of the cells from which it was derived. (microbiologyresearch.org)

The target is the basic genetic material (genome) of the cell, the deoxyribonucleic acid (DNA). (invitrojobs.com)
Both crRNA-tracrRNA duplexes and sgRNAs can be used to target SpCas9 for multiplexed genome editing in eukaryotic cells 1 , 3 . (cdc.gov)

It is noted that in-vitro studies have given conflicting results concerning the carcinogenic potential of TCE. (cdc.gov)
On 14 March 2011, the European Union Reference Laboratory for Alternatives to Animal Testing (EURL ECVAM), hosted by the European Commission's Joint Research Centre (JRC) , issued a recommendation concerning three in vitro test methods for assessment of the carcinogenic potential of chemical substances . (ritsq.org)

Description: A competitive ELISA for quantitative measurement of Porcine True insulin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (chipready.com)

One in vitro study suggested that ciclopirox acts by chelation of polyvalent cations (Fe +3 or Al +3 ) resulting in the inhibition of the metal-dependent enzymes that are responsible for the degradation of peroxides within the fungal cell. (guidelinecentral.com)
In a preclinical study published in the journal Frontiers in Pharmacology , researchers from the USA investigated the potential chemopreventive effects of a mushroom - Ganoderma lucidum (GL)-derived commercial product named GLSF in vitro and in mice with lung carcinogenesis induced by two tobacco smoke carcinogens, namely benzo[a]pyrene (B[a]P) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). (news-medical.net)
In the short-term exposure study in mice, B[a]P was found to disrupt the architecture of the lung and alveoli while causing an increased infiltration of inflammatory cells as compared to the control group. (news-medical.net)
In this study, we administered fractionated RT combined with HBOC to treat Miapaca-2 cell and Hela cell xenografts on nude mice. (bvsalud.org)
This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)

The short labeling and total assay times reduces the potential for significant changes in the number and/or phenotype of the target cells, which could influence the outcome of the assay. (jove.com)
Correlation between in vitro MIC results and clinical outcome has yet to be established for ciclopirox. (guidelinecentral.com)

Blood samples (n=31,234) were collected from children aged 0-14 years during a 2018 nationwide HIV survey and assayed for Plasmodium antigenemia, Plasmodium DNA, and IgG against Plasmodium MSP1-19 antigens. (cdc.gov)
However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)

Additionally, the in vitro antiparasitic activity of eight clinically used dihydropyridines (azelnidipine, amlodipine, cilnidipine, lercanidipine, nicardipine, nifedipine, nimodipine, and nitrendipine) was demonstrated against a panel of Leishmania species and Trypanosoma cruzi [ 6 , 7 ]. (hindawi.com)

Methods: MDA MB231 breast cancer cells stably overexpressing wild type form of Tm1 or Tm1 mutants (S283A and S283E) were generated. (scirp.org)

cell cycle delay observed through 16.7 µg/mL - with metabolic activation. (europa.eu)
Without metabolic activation the test item was negative under the experimental conditions of this assay. (europa.eu)

Adhesion assays were performed at various periods of time on cells grown on plastic. (scirp.org)
Control cells cultured for the same periods of time as MNU-treated cells produced only ductal outgrowths that were morphologically similar to those found in the mammary glands of adult virgin hosts. (eurekamag.com)

The results showed that CRABP2 was highly expressed in cervical carcinoma tissues and cell lines, and its high expression was associated with poor overall survival. (bvsalud.org)
The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. (mytaq.com)

In the 5% strength, it is also useful in the treatment of superficial basal cell carcinomas when conventional methods are impractical, such as with multiple lesions or difficult treatment sites. (nih.gov)
The diagnosis should be established prior to treatment, since this method has not been proven effective in other types of basal cell carcinomas. (nih.gov)
Abnormal DNA content has been related to aggressive behavior in Mucoepidermoid Carcinomas (MEC), Adenoid Cystic Carcinomas (AdCC), Acinic Cell Carcinomas (ACC) and oncocytomas (Onc) [8]. (researchgate.net)
Jelaković B, Castells X, Tomić K, Ardin M, Karanović S, Zavadil J. Renal cell carcinomas of chronic kidney disease patients harbor the mutational signature of carcinogenic aristolochic acid. (who.int)

The kit can be also used in culturing cells in 3D collagen matrix. (mytaq.com)

Thus, having an assay in which the user can easily integrate any number of different effector cells, target cells, and/or response modifiers into the experimental design can serve as a valuable means of quickly assessing the cytotoxic capacity of effector cells and/or the responsiveness of the target cell. (jove.com)

METHODS OF SLIDE PREPARATION AND STAINING TECHNIQUE USED: Cells were trypsinised and suspended in a hypotonic solution (37.5 mM KCl) for 10 minutes at 37°C. After centrifugation (800 to 1000 rpm) the cell pellets were fixed in three steps with glacial acetic acid/ methanol 1 + 3 and stored overnight at 4°C. The next day the cells were spread on wet and ice cold slides and stained. (europa.eu)
Based on a rangefinding assay a 20h harvest time was chosen for the aberration assay with concentrations ranging from 37.5 µg/mL through 500 µg/mL. (europa.eu)

In vitro methodologies employing various broth or solid media with and without additional nutrients have been utilized to determine ciclopirox minimum inhibitory concentration (MIC) values for the dermatophytic molds. (guidelinecentral.com)
The cells were seeded into four parallel plastic tissue culture flasks at a concentration of 300000 cells per flask (7 hour interval) and 150000 cells per flask (18 and 28 hour intervals). (europa.eu)
In vitro studies showed that over the concentration range of 0.1 to 100 mcg per mL, the amount of lamivudine associated with erythrocytes ranged from 53% to 57% and was independent of concentration. (drugcentral.org)

The addition of a coverslip layer to the microfluidic incubation chamber prevents evaporation of droplets which allows imaging of encapsulated cells on-chip that self organize into a monolayer. (uic.edu)

SETD2 loss causes growth arrest and differentiation of AML cells, and leads to increased DNA damage. (nature.com)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repres- sion and transcriptional activation. (lu.se)

Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibiting mouse cells. (microbiologyresearch.org)

Anatomy11

Organisms11

Diseases5

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes36

Disciplines and Occupations1

Information Science3

Vivo8

Proliferation20

Mammalian Chromosome Aberration Test1

Micronucleus assay1

Flow Cytometry3

Toxicity8

Malignant5

Cytotoxicity2

Chinese hamst1

Salmonella3

Mutagenicity3

Cellular8

Embryo cells3

Tumor cells5

Biolabs3

Kinase3

Apoptosis5

Agar4

Boyden2

Luciferase1

Bacterial3

Migration11

Mutation2

Chromosomal1

Antigen4

Locus2

Epithelial4

BALB1

Strains2

Susceptibility1

Chromosome1

Bone marrow3

Immune8

Tumors5

Inhibitors1

Experiments1

Detect1

Populations4

Viability1

Protein5

Assess1

Membrane1

Fibroblasts1

Genome2

Carcinogenic potential2

Culture supernatant1

Study5

Outcome2

Samples2

Species1

Stably1

Metabolic activation2

Periods of time2

Tissues2

Carcinomas4

Collagen1

Experimental1

37.52

Concentration3

Chamber1

Differentiation2

Viruses1