• Subsequently, bioactivity assays were evaluated in vitro using the TF-1 tyrosine receptor kinase A (Trk-A) expressing cell line. (frontiersin.org)
  • Fusions involving the tropomyosin-related kinase (TRK) family of neurotrophin receptor tyrosine kinases have been identified in various human cancers and suggested to function as oncogenic drivers. (aacrjournals.org)
  • DMH-1 is a selective inhibitor of bone morphogenic protein (BMP) type-I receptor activin receptor-like kinase 2 (ALK2) receptor (IC 50 = 108 nM or 12.6 nM in in vitro kinase assays). (tocris.com)
  • DMH-1 is also offered as part of the Tocriscreen 2.0 Max , Tocriscreen Kinase Inhibitor Library and Tocriscreen Stem Cell Library . (tocris.com)
  • This phase I trial studies the best dose and side effects of CD19/CD22 chimeric antigen receptor (CAR) T cells when given together with chemotherapy, and to see how well they work in treating children or young adults with CD19 positive B acute lymphoblastic leukemia that has come back or does not respond to treatment. (stanford.edu)
  • A CAR is a genetically-engineered receptor made so that immune cells (T cells) can attack cancer cells by recognizing and responding to the CD19/CD22 proteins. (stanford.edu)
  • The trk receptors in general are expressed in a nonoverlapping manner by sensory neurons in combination with the low-affinity neurotrophin receptor p75 ( Wright and Snider, 1995 ). (jneurosci.org)
  • This inhibits cleavage of the sialyl-acid bond to the host receptor, thus the virus is unable to be released from infected host cells and spread to new cells. (who.int)
  • DMH-1 induces intestinal differentiation in human intestinal organoids (hIOs) derived from human pluripotent stems cells (hPSCs). (tocris.com)
  • In vitro proliferation and differentiation of haMSCs were not affected by the labeling of DiD dye. (biomedcentral.com)
  • Here we show the differentiation of patient specific iPSCs derived from a patient with SFTPB deficiency into lung organoids with mesenchymal and epithelial cell populations from both the proximal and distal portions of the human lung. (nature.com)
  • After differentiating the mutant and corrected cells into lung organoids, we show expression of SFTPB mRNA during endodermal and organoid differentiation but the protein product only after organoid differentiation. (nature.com)
  • Although many differentiation protocols in the literature have been successful in mimicking lung development from stem cells, there has not been an examination of how a specific mutation impacts the differentiation process including its effects on the early endoderm, as well as the proximal and distal lung epithelial cell populations in the lung organoids. (nature.com)
  • The 3dGRO™ Human Lung Organoid Culture System is a serum-free, multi-stage culture system for efficient differentiation of human induced pluripotent stem (iPS) cells to mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. (sigmaaldrich.com)
  • The brain spheroids are generated by non-directed differentiation of neural stem cells derived from human iPS cells (NSC hiPS ) 3 . (hes-so.ch)
  • In vitro cultivated stem cell populations are in general heterogeneous with respect to their expression of differentiation markers. (biomedcentral.com)
  • Here, we study MSC plasticity on the level of individual cells applying a multi-scale computer model that is based on the concept of noise-driven stem cell differentiation. (biomedcentral.com)
  • The generation and maintenance of replenishing tissues relies on an appropriately regulated balance between self-renewal and differentiation within a relatively small population of adult stem cells. (biomedcentral.com)
  • According to this concept, MSC plasticity bases on permanent fluctuations of the differentiation state of each individual cell, which enables more differentiated cells to re-gain stem cell properties and subsequently to switch lineage (details see below). (biomedcentral.com)
  • The proliferation and differentiation of pluripotent stem cells give rise to progeny that can populate the entire immunologic and hematopoietic systems through committed progenitors of both the lymphoid and myeloid lineages. (medscape.com)
  • We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
  • Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
  • Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
  • Human adipose-derived mesenchymal stem cells (haMSCs) have shown efficacy in treating osteoarthritis (OA) both preclinically and clinically via intra-articular (IA) injection. (biomedcentral.com)
  • The current study represents the first attempt to correlate cell therapy efficacy on OA with the physical presence of the injected haMSCs in the OA model, and demonstrates that human adipose-derived mesenchymal stem cells persisted for 10 weeks locally in the rat joint, coinciding with the efficacy observed. (biomedcentral.com)
  • We have characterized equine, canine and feline stem cells isolated from different tissues (i.e. fat, bone marrow, umbilical cord blood and umbilical cord tissue) with a focus on adult-derived mesenchymal stem cells. (ucdavis.edu)
  • This kind of plasticity has been suggested to be a fundamental feature of mesenchymal stem cells (MSCs) as well. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are multi-potent cells that persist in adult life in some tissue types, such as bone-marrow stroma, fat, skeletal muscle, and synovium without loosing their capacity to proliferate and differentiate [ 9 , 10 ]. (biomedcentral.com)
  • Organ-on-chips refer to the cell culture devices based on microfluidic technology to simulate the physiological functions and behaviors of tissues and organs through arranging living cells in a micrometer-sized chamber and the continuous supply of cell culture media. (selectbiosciences.com)
  • Unlike traditional immortalized pulmonary cell lines and primary cells, lung organoids contain various differentiated cell types with complex tissue architecture that more closely resemble in vivo tissues and functionality. (sigmaaldrich.com)
  • Additionally, lung organoids can be derived from small amounts of patient tissues or pluripotent stem cells to create living biobanks that facilitate personalized biomedical research. (sigmaaldrich.com)
  • The cortical structure was made from human induced pluripotent stem cells (hiPSCs), which have the potential to produce the cell types found in most human tissues. (ox.ac.uk)
  • The new advance builds on the team's decade-long track record in inventing and patenting 3D printing technologies for synthetic tissues and cultured cells. (ox.ac.uk)
  • Stress-adaptive cell plasticity in target tissues and cells for plant biomass growth is important for yield stability. (frontiersin.org)
  • Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
  • Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
  • The abundance of neutrophils in human circulation, their fast mobilization from blood to tissues, along with their alleged short life-span led to the image of neutrophils as a homogeneous cell type designed to fight infections and die in the process. (bvsalud.org)
  • To better understand Plasmodium biology, researchers can whether proceed to in vitro studies or use in vivo models. (wjgnet.com)
  • Current in vivo models use mainly rodents and replacement with predictive vitro models are still lacking. (hes-so.ch)
  • To achieve our objectives, we use start of the art next-generation sequencing technologies, in vitro and in vivo models of acute leukemia, cell sorting, single-cell sequencing and advanced bioinformatics. (lu.se)
  • The validation of the in vitro TBI platform is done by using complementary methods: electrical monitoring, imaging (confocal microscopy and optical projection tomography), and circulating biomarkers analysis by multiplexing assays 4 . (hes-so.ch)
  • Among them, in vitro assays have been quantitative and expeditious as initial approximations that warrant further confirmation in vivo . (news-medical.net)
  • A majority of endothelial cell assays utilize human umbilical vein endothelial cells or bovine aortic endothelial cells - not because these types of cells are good representatives of vascular conditions in vivo , but primarily because they are relatively easy to harvest from large blood vessels. (news-medical.net)
  • Therefore in vitro angiogenesis assays should be viewed as a starting point rather than an endpoint for discovery. (news-medical.net)
  • The transwell migration assay (a modification of the classical Boyden chamber assay) is frequently utilized to assess endothelial cell migration, which is one of the main in vitro assays in the repertoire of Thermo Fisher Scientific. (news-medical.net)
  • Other variants of migration assays include under-agarose assay, Teflon fence assay, wound healing assay and phagokinetic track assay. (news-medical.net)
  • At the moment, the most popular tube formation assays involve plating human umbilical vein endothelial cells or bovine aortic endothelial cells with Matrigel. (news-medical.net)
  • Other in vitro angiogenesis assays that cover all angiogenic functions ( i.e. proliferation, migration, tube formation) include mouse metatarsal assay, embryoid assay and others. (news-medical.net)
  • Blitterswijk graduated from a bachelor in cell biology at Leiden University and defended his PhD thesis in 1985 at the same university on artificial ceramic middle ear implants under the supervision of Professor Jan Grote and Klaas de Groot, for which he was awarded the Jean Leray young scientist award from the European Society for Biomaterials in 1987. (wikipedia.org)
  • Radiation biology endpoints are usually evaluated using standardized procedures such as the colony formation assay for proliferation or the caspase 3/7 sytox assay for cell death. (unibw.de)
  • Our research group utilizes state of the art methods in biochemistry, cell biology and molecular biology. (ruhr-uni-bochum.de)
  • 2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
  • They have succeeded admirably, and as a bonus the book provides a fascinating insight into where the technical limits lie, of how we stand as the authors suggest in their introduction at the beginning of a new era of single-molecule-based cell biology. (cshlpress.com)
  • Cell Chemical Biology. (lu.se)
  • We also show the presence of normal lamellar bodies and the secretion of surfactant into the cell culture medium in the organoids of lentiviral infected cells. (nature.com)
  • Two years ago, the team led by Charna Dibner, Principle Investigator in the Departments of Medicine and of Cell Physiology and Metabolism, and Diabetes Centre at UNIGE Faculty of Medicine, and at HUG, has already shown that in rodents the perturbation of pancreatic cellular clocks led to disrupted insulin and glucagon secretion, thus promoting the onset of diabetes. (sciencedaily.com)
  • We had also previously observed that if the clocks of human pancreatic cells were artificially disrupted in the cellular culture in vitro, secretion of the key islet hormones -- insulin and glucagon -- was compromised," says Volodymyr Petrenko, a researcher in Dr. Dibner's lab and the first author of these publications. (sciencedaily.com)
  • Moreover, the defects in temporal coordination of insulin and glucagon secretion observed in patients with type-2 diabetes were comparable to those measured in healthy islet cells with artificially-disrupted circadian clock. (sciencedaily.com)
  • In vitro systems with reproducible cell plasticity can help to identify relevant metabolic and molecular events during early cell reprogramming. (frontiersin.org)
  • Our predictions on MSC plasticity and its dependence on the environment motivate a number of in vitro experiments for validation. (biomedcentral.com)
  • Currently, there is an ongoing debate on fundamental dynamics underlying this kind of cell plasticity. (biomedcentral.com)
  • We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
  • All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
  • Hematopoietic cells are almost totally absent. (medscape.com)
  • The microenvironment, consisting of lymphocytes, macrophages, fibroblasts, endothelial elements, and stroma in which hematopoietic cells reside, creates a regulatory niche that determines the local area network. (medscape.com)
  • Accelerated hematopoietic cell apoptosis has been demonstrated in virtually all IBMFS. (medscape.com)
  • In some types of angiogenesis, endothelial cells degrade the basement membrane and migrate along chemical gradients previously established by proangiogenic growth factors. (news-medical.net)
  • One of the highly specific tests for angiogenesis is assessing the propensity of endothelial cells to form 3D structures (also known as tube formation). (news-medical.net)
  • Taken together, the inhibition of hsa_circ_0046060 expression in exosomes from GDM-derived UMSCs can alleviate GDM by reversing abnormal glucose metabolism and insulin resistance in vivo and in vitro . (hindawi.com)
  • The researchers demonstrated for the first time that neural cells can be 3D printed to mimic the architecture of the cerebral cortex. (ox.ac.uk)
  • Up to now, however, there has been no method to ensure that implanted stem cells mimic the architecture of the brain. (ox.ac.uk)
  • We demonstrate that our innovative combination of human 3D minibrains and in vitro device can mimic brain injury. (hes-so.ch)
  • As an alternative approach, Zill and colleagues prospectively assessed the feasibility and efficacy of cell-free DNA (cfDNA) sequencing in 26 patients with advanced pancreatic or biliary carcinomas. (aacrjournals.org)
  • The goal of this project is to enhance treatment efficacy by individual cocooning of bone marrow stem cells in bioengineered collagen-based microspheres. (europa.eu)
  • The cocooned cells will be tested in vitro for their safety and efficacy (e.g. spheres degradation rate, cytotoxicity), and in vivo in MI mice models for their effectiveness in restoring heart functions by echocardiography, and for their traceability by dual-energy CT. (europa.eu)
  • We attempted to reveal the existence of labeled cells in vivo by imaging and a molecular biomarker approach, and to correlate with the in vivo efficacy and physical presence over a follow-up period up to 10 weeks. (biomedcentral.com)
  • 10 5 cells) lasted for about 10 weeks in the surgical knee joint at the same time as efficacy was observed. (biomedcentral.com)
  • To this end, supported by research demonstrating superior antitumor function of naïve derived effector T cells compared to central memory derived effector T cells, I am investigating whether preferential modification of naïve T cells to express CARs will generate a T cell subpopulation with increased efficacy. (stanford.edu)
  • [ 3 ] Clinical trials are currently under way on the safety, tolerability, and efficacy of transplanting such cells in encapsulated forms in persons with type 1 diabetes, with results to be announced in the coming years. (medscape.com)
  • Tracking translocation of industrially relevant engineered nanomaterial s across alveolar epithelial monolayers in vitro. (cdc.gov)
  • Surfactant is produced by alveolar type II cells which can be differentiated in vitro from patient specific induced pluripotent stem cell (iPSC)-derived lung organoids. (nature.com)
  • Alveolar type II (ATII) epithelial cells synthesize, secrete and recycle all components of surfactant and dysfunction in surfactant metabolism can result in a variety of pediatric lung diseases including respiratory distress syndrome and interstitial lung disease 3 . (nature.com)
  • AFE cells may be cryopreserved (SCC301) or further differentiated into branching lung bud organoids using 3dGRO™ Lung Organoid Branching Medium (SCM307) and further matured into branching and alveolar lung organoids using 3dGRO™ Lung Organoid Maturation Medium (SCM308). (sigmaaldrich.com)
  • The urgent need for novel treatments inspired me to pursue a research project in adoptive immunotherapy, genetically modifying Tcells to express artificial T cell receptors, termed chimeric antigen receptors (CARs), that target AML specific antigens. (stanford.edu)
  • To allow precise quantitation of translocation across lung epithelial cells, we developed a method for tracking metal oxide ENMs in vitro using neutron activation. (cdc.gov)
  • The versatility and sensitivity of the proposed in vitro epithelial translocation (INVET) system was demonstrated using a variety of industry relevant ENMs including CeO2 of various primary particle diameter, ZnO, and SiO2-coated CeO2 and ZnO particles. (cdc.gov)
  • Calu-3 lung epithelial cells cultured to confluency on transwell inserts were exposed to neutron-activated ENM dispersions at sub-lethal doses to investigate the link between ENM properties and translocation potential. (cdc.gov)
  • Previous studies on the impact of CyaA on human epithelial cells have been carried out using cell lines derived from the airways or the intestinal tract. (uni-wuerzburg.de)
  • Here, we investigated the interaction of CyaA and its enzymatically inactive but fully pore-forming toxoid CyaA-AC- with primary human airway epithelial cells (hAEC) derived from different anatomical sites (nose and tracheo-bronchial region) in two-dimensional culture conditions. (uni-wuerzburg.de)
  • We can isolate soluble as well as membrane bound protein complexes and study their assembly, and can also purify heterologously expressed proteins from bacteria in order to study protein-protein interactions or ubiquitination reactions in vitro. (ruhr-uni-bochum.de)
  • Single molecule optical techniques will be applied to track and study the function of single proteins in vitro and in live cells. (cipsm.de)
  • In a process that was dependent upon the presence of infectious VZV, these proteins rapidly became sequestered in the cytoplasm of VZV-infected cells. (simulations-plus.com)
  • We suggest that VZV has evolved a mechanism to limit host cell antiviral defenses by sequestering NF-kappaB proteins in the cytoplasm, a strategy that appears to be unique among the herpesviruses. (simulations-plus.com)
  • Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
  • These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)
  • But what we are beginning to see is that there are also systems in which every cell can sense the nutrient condition in their own neighborhood and adjust their metabolism to their local nutrient conditions. (vetscite.org)
  • A detailed modeling & simulation program for in vitro metabolism & transport studies. (simulations-plus.com)
  • The TRK inhibitor LOXO-101 inhibits the growth of TRK fusion-positive cells in vitro and in vivo . (aacrjournals.org)
  • Induced by food deprivation, it inhibits stress-induced apoptotic cell death in vitro and promotes fat mobilization in vitro and in vivo. (vetscite.org)
  • These findings suggest that a lethal lung disease can be targeted and corrected in a human lung organoid model in vitro . (nature.com)
  • This approach was thought feasible on the basis of tests performed using fluorescence microscopy, where fluorescent molecule were used to light up the stem cells of interest. (ca.gov)
  • Techniques range from in vivo and in vitro fluorescent based methods to the use of atomic force microscopy, optical and magnetic tweezers, and nanopores. (cshlpress.com)
  • The orally available, highly selective small-molecule TRK family inhibitor LOXO-101 is currently being tested in a phase I dose-escalation study in patients with advanced solid tumors. (aacrjournals.org)
  • Add ROCK inhibitor (ROCKi) Y-27632 ( SCM075 ) to 7-10 mL Human ES/iPS cell Expansion Media ( SCM130 ) to a final concentration of 10 µM. (sigmaaldrich.com)
  • The cellular structures and viral particles, observed by transmission electron microscopy, indicated that macrophages and spermatogonial cells are the main SARS-CoV-2 lodging sites, where new virions form inside the endoplasmic reticulum Golgi intermediate complex. (bvsalud.org)
  • CONCLUSIONS: Our findings indicate that high angiotensin II levels and activation of mast cells and macrophages may be critical for testicular pathogenesis. (bvsalud.org)
  • DMH-1 suppresses lung cancer cell proliferation, migration, invasion in vitro and reduces tumor growth in a mouse lung cancer xenograft model. (tocris.com)
  • Those are degradation of adjacent matrix, migration toward the angiogenic stimuli, proliferation to create additional cells for the new blood vessels, as well as reorganization to form 3D vessel structures. (news-medical.net)
  • 24- or 96-multiwell BD FluoroBlok insert (with 3 μm pore size) coated with human fibronectin was developed by a company BD Biosciences, and also serves as a cell migration assay. (news-medical.net)
  • In vitro, PBM induced VEGF upregulation, improved dental pulp cells proliferation and migration, and favored their mineralization potential. (bvsalud.org)
  • The In Vitro Pyrogen Test using MonoMac 6 cells/IL-6 (MM6-IL6) is a monocyte activation test (MAT) applied to detect or quantify substances that activate human monocytes or monocytic cells (here the cell line MonoMac 6) to release pro-inflammatory cytokines, for example tumour necrosis factor alpha (TNFα), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). (europa.eu)
  • Regenerative cell-based therapies have shown promising results for a variety of diseases, including radiation-therapy-induced xerostomia, yet practical methods for tracking transplanted cells are still lacking. (ca.gov)
  • In addition, these methods require special treatments of the cells with, among other things, chemicals that affect the reactions in the cell. (unibw.de)
  • Methods We treated cell cultures with BC/BL or defined single plant extracts, purified anthocyanins and astaxanthin in different concentrations and subsequently infected the cultures with the Measles virus (wild-type or vaccine strain Edmonston), Herpesvirus 1 or 8, or murine Cytomegalovirus. (uni-wuerzburg.de)
  • Methods We established new patient-derived PA cell lines that preserve molecular features of the primary tumors and can be studied in OIS and proliferation depending on expression or repression of the SV40 large T antigen. (ibecbarcelona.eu)
  • This Notice of Funding Opportunity (NOFO) supports Phase I (R43), Phase II (R44), Direct to Phase II (R44), and Fast Track (R44) Small Business Innovative Research (SBIR) grant applications from small businesses concerns (SBCs) to develop resources, new methods, and approaches that can be applied in testing strategies to better understand the role of environmental chemicals in the etiology of the neurodevelopmental disorders. (nih.gov)
  • There are a myriad of well-established in vitro methods for studying cell proliferation. (news-medical.net)
  • Shengli Mi received his PhD in Stem cell and Tissue Engineering from Northwest University of Agriculture and Forestry. (selectbiosciences.com)
  • He is mainly engaged in research work in the field of biological manufacturing and tissue engineering, and his research interests include biofabrication, disease pathogeneses study, drug test and discovery, and cell/tissue/organ-on-a-chip devices. (selectbiosciences.com)
  • Despite numerous attempts at injecting stem cells into post-MI heart to affect regeneration, the consensus is that anoikis (cell death) induced by the lack of contact between the cells and the tissue scaffold during injection, the harsh environment at the injection site, and powerful myocardial contractions cause massive cell loss rendering the therapy ineffective. (europa.eu)
  • The cocoon provides the tissue support for cell survival, promotes integrin upregulation for better engraftment of the cells onto the heart tissue, and protect the cells from the harsh post-MI environment. (europa.eu)
  • Researchers have produced an engineered tissue representing a simplified cerebral cortex by 3D printing human stem cells. (ox.ac.uk)
  • Tissue regenerative therapies, especially those in which patients are given implants derived from their own stem cells, could be a promising route to treat brain injuries in the future. (ox.ac.uk)
  • In this new study, the University of Oxford researchers fabricated a two-layered brain tissue by 3D printing human neural stem cells. (ox.ac.uk)
  • When implanted into mouse brain slices, the cells showed convincing structural and functional integration with the host tissue. (ox.ac.uk)
  • A key advantage of using hiPSCs for tissue repair is that they can be easily derived from cells harvested from patients themselves, and therefore would not trigger an immune response. (ox.ac.uk)
  • We are building up a mTBI in vitro platform based on controlled expulsions of culture medium on 3D neural tissue from human iPS cells. (hes-so.ch)
  • Pericytes and glial cells are known to collaborate in dental pulp tissue repair. (bvsalud.org)
  • Herein, different subsets of perivascular cells were unveiled in the pulp tissue. (bvsalud.org)
  • Such disturbances seem to prevent the proper functioning of the cells in the pancreatic islet that secrete insulin and glucagon, the hormones that regulate blood sugar levels. (sciencedaily.com)
  • In type 2 diabetes , beta cells fail to secrete sufficient insulin to overcome the prevailing insulin resistance . (medscape.com)
  • Functional analyses use stem cells and other biological systems in vitro . (jax.org)
  • In biological radiation research, cellular responses after irradiation are usually studied in vitro, i.e. on cells in so-called cell culture. (unibw.de)
  • Yet that is exactly what the pair achieved: engineering dots that are small enough (10-15 nm in diameter) to track the biological processes inside the nucleus of live HeLa cells. (the-scientist.com)
  • The biological rhythms of the islet cells in type-2 diabetes exhibit both reduced amplitudes of circadian oscillations and poor synchronization capacity. (sciencedaily.com)
  • The Molecular Landscape of Hürthle Cell Thyroid Cancer Is Associated with Altered Mitochondrial Function-A Comprehensive Review. (nih.gov)
  • The molecular mechanisms by which such stem cells preserve the ability to switch to a pluripotent state are being investigated. (ny.gov)
  • IkappaBalpha levels did not diminish even though the protein became phosphorylated and ubiquitinated, as determined based on detection of the characteristic high-molecular-weight form of the protein, and the 26S proteasome remained functional in VZV-infected cells. (simulations-plus.com)
  • Nearly all the cells in our body comprise molecular clocks that regulate and synchronize metabolic functions to a 24-hour cycle of day-night changes. (sciencedaily.com)
  • Using combined bioluminescence-fluorescence time-lapse microscopy, a technology that allows tracking the molecular clock activity in living cells very precisely over time, the scientists compared the behaviour of pancreatic cell of type-2 diabetic donors and those of healthy subjects throughout the day. (sciencedaily.com)
  • Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
  • Normal male germline stem cells exhibit striking phenotypic similarities to adult human germ cell tumor, the pathogenesis of which is also a major emphasis of the research. (ny.gov)
  • Phenotypic variability shown in in vitro culture systems is due to high genotype dependence, going from species level to the level of cultivar/variety and individual genotypes. (frontiersin.org)
  • Scientists tracked down a comparative pattern with other tumor cells, recommending that curcumin may not be the lone powerful plant compound in turmeric 95 curcuminoid . (empowher.com)
  • Conclusions Our data demonstrate that BCL-XL is critical for survival of senescent PA tumor cells and provides proof-of-principle for the use of clinically available BCL-XL-dependent senolytics. (ibecbarcelona.eu)
  • Promotes neurogenesis in human induced pluripotent stem cells (iPSCs) when used in combination with SB 431542 (Cat. (tocris.com)
  • They are 3-dimensional, complex, multicellular structures that can be derived from patient specific human induced pluripotent stem cells (hiPSCs). (nature.com)
  • Human pluripotent stem cells are differentiated into definitive endoderm cells using a 4-day induction medium. (sigmaaldrich.com)
  • In this study we describe the generation and basic functional characteristics of mDA neurons derived from a human pluripotent stem cell (hPSC) line expressing eGFP under the control of the PITX3 promoter. (monash.edu)
  • This indicates that the human and mouse cells were communicating with each other, demonstrating functional as well as structural integration. (ox.ac.uk)
  • Our findings point to DcAOX1 and DcAOX2a as being reasonable candidates for functional marker development related to early cell reprogramming. (frontiersin.org)
  • Simulation results on the composition of the functional stem cell pool of MSC populations suggest that most of the cells that constitute this pool originate from more differentiated cells. (biomedcentral.com)
  • This team showed that injection of just three genes- Neurog3, Mafa, and Pdx1 -into the pancreatic parenchyma of mice leads to conversion of exocrine cells to functional beta cells, a process popularly known as "reprogramming. (medscape.com)
  • [ 7 , 8 ] As background, in 2009, Collombat and colleagues [ 9 ] showed that pancreatic misexpression of the gene Pax4 led to the conversion of glucagon -producing alpha cells into functional beta cells. (medscape.com)
  • Novel genetic approaches have been implemented to identify and prospectively track primitive germ cell populations in the adult testis of both mice and humans, using a combination of in vitro and in vivo systems. (ny.gov)
  • Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
  • However, the organisation of stem cell populations is strongly influenced by environmental factors such as specific cell-cell interactions, growth factor and oxygen supply, as well as the geometry and mechanical properties of the local environment [ 3 , 4 ]. (biomedcentral.com)
  • Dependence of senescent PA cells on anti-apoptotic BCL-2 members was investigated using a comprehensive set of BH3 mimetics. (ibecbarcelona.eu)
  • In type 1 diabetes , beta cells succumb to autoimmunity, leaving individuals with significantly reduced beta-cell mass and a lifelong dependence on insulin replacement therapy. (medscape.com)
  • Using careful, state-of-the-art cell tracking techniques, the same group subsequently showed that Pax4 misexpression initially caused the conversion of cells lining the ducts to become alpha cells, which were then converting to beta cells. (medscape.com)
  • This enables us to track individual transport events by the use of selective markers and inhibitors by applying fluorescence microscopy as well as biochemical subcellular fractionation. (ruhr-uni-bochum.de)
  • In 2015, he was awarded an ERC Advanced grant that aimed at developing microfabricated and microfluidic cell culture platforms for improving organoids. (wikipedia.org)
  • Using three-dimensional live-cell imaging of patient-derived tumor organoids (tumor PDOs), we show that CIN is widespread in colorectal carcinomas regardless of background genetic alterations, including microsatellite instability. (nature.com)
  • Lung organoids are useful three-dimensional (3D) cell culture models for studying human lung development and respiratory diseases including viral infections (SARS-CoV, H1N1, MERS), cystic fibrosis, asthma/COPD, exposure to air pollution and the effects of smoking. (sigmaaldrich.com)
  • The hiPSCs were differentiated into neural progenitor cells for two different layers of the cerebral cortex, by using specific combinations of growth factors and chemicals. (ox.ac.uk)
  • The etiology of bone marrow failure (BMF) includes defective stem/progenitor cells and/or stroma/accessory cells/growth factors, as well as deficient nonspecific nutrients or, as in the case of acquired aplastic anemia, immune-mediated abnormalities. (medscape.com)
  • By comparing the pancreatic cells of type 2 diabetic human donors with those of healthy people, researchers at the University of Geneva (UNIGE) and at the University Hospitals of Geneva (HUG), Switzerland, were able to demonstrate, for the first time, that the pancreatic islet cells derived from the Type 2 Diabetic human donors bear compromised circadian oscillators. (sciencedaily.com)
  • A mechanistic simulation engine for the in vitro dissolution experiment that predicts in vitro disintegration and dissolution of pharmaceutical dosage forms. (simulations-plus.com)
  • Through an in vitro experiment exposing VERO cells to testicular macerates, we observed viral content in all samples, and the subgenomic RNA's presence reinforced the replicative activity of SARS-CoV-2 in testes of the severe COVID-19 patients. (bvsalud.org)
  • BH3 mimetics with high affinity for BCL-XL (BCL-XLi) reduce metabolic activity and induce mitochondrial apoptosis in senescent PA cells at nano-molar concentrations. (ibecbarcelona.eu)
  • The compounds suppressed SARS-CoV-2 replication in cell culture cells and a patient-near replication system using human precision-cut lung slices by two orders of magnitude. (uni-wuerzburg.de)
  • Infection of iPSCs with lentiviral inserts is a highly efficient process since stem cells grow quickly, remain undifferentiated in specific cell culture conditions and can establish fully infected clones within 2-3 passages. (nature.com)
  • The in vitro TBI device is inducing injury by a fine tunable (speed and force) expulsion of culture medium onto the minibrain by using a microvalve ( SMLD 300G , Fritz Gyger AG) . (hes-so.ch)
  • Platform&Trauma: The in vitro TBI device is inducing injury by a finely tunable (speed and force) expulsion of culture medium onto the minibrain (Fig. 1A). (hes-so.ch)
  • Monitoring the fates of individual cells in high and low oxygen culture, we calculated the average transition times of individual cells into stem cell and differentiated states. (biomedcentral.com)
  • Under appropriate culture conditions, they can multiply and transform into specialized cell types in vitro . (biomedcentral.com)
  • Cell culture isolation of SARS-CoV-2 is possible, but the Centers for Disease Control and Prevention (CDC) recommends that clinical laboratories not attempt this unless it is performed in a biosafety level 3 (BSL-3)-certified laboratory. (medscape.com)
  • This new tool will benefit Californians by accelerating progress toward cures in ongoing CIRM-sponsored trials of stem cell therapies. (ca.gov)
  • The development of targeted therapies in pancreatobiliary cancers has been hampered by the difficulty in obtaining biopsies with sufficient tumor cell content for genomic sequencing analyses. (aacrjournals.org)
  • We have developed collaborative, interdisciplinary "disease teams" that include basic research faculty and clinical faculty that focus on "bench to bedside" translation of stem cell therapies. (ucdavis.edu)
  • Cell-based therapies that stimulate these stromal components may be of therapeutic relevance for partially vital dental pulp conditions. (bvsalud.org)
  • The third project is focused on identifying targets affecting leukemia stem cells and on providing in vivo proof of concept data for targeted therapies with a focus on antibody-based strategies. (lu.se)
  • Although a few FDA-approved therapies for type 2 diabetes (meglitinides, sulfonylureas, incretin-based drugs) directly enhance beta-cell function, no therapies for any form of diabetes lead to the growth of healthy, new beta cells to replace those that were lost or dysfunctional. (medscape.com)
  • In these experiments, we demonstrated that VZV interferes with NF-kappaB activation in cultured fibroblasts and in differentiated epidermal cells in skin xenografts of SCIDhu mice infected in vivo. (simulations-plus.com)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • Moreover, using clock modulator molecule dubbed Nobiletin, extracted from lemon peel, the researchers succeeded in "repairing" the disrupted cellular clocks and in partial restoring of the islet cell function. (sciencedaily.com)
  • The investigators showed that a class of antimalarial drugs known as artemisinins, typified by the FDA-approved drug artemether , were capable of inhibiting ARX levels, reducing glucagon, and increasing insulin in an in vitro alpha-cell model. (medscape.com)
  • Inherited bone marrow failure syndromes (IBMFS) are usually the result of intrinsic stem cell/progenitor defects. (medscape.com)
  • Microscopy with phase contrast also has the advantage that no dyes or other treatments are necessary, allowing the cells to be observed completely undisturbed for several days. (unibw.de)
  • Tracking eGFP using time lapse confocal microscopy over 24 h demonstrated significant TNF-mediated neurite retraction over time. (monash.edu)
  • The injured area is then 3D spatially mapped within the injured minibrain by Optical Projection Tomography (OPT) (Fig. 1D) and confocal microscopy (Fig. 1E) after staining with cell-permeant and non-permeant nuclear dye. (hes-so.ch)
  • Here we review evidences arising especially from intravital microscopy studies that demonstrate neutrophils as cells endowed with sophisticated mechanisms and able to engage in complex interactions as to minimize damage and optimize their effector functions. (bvsalud.org)