EmbryosReprodVivo2001FetalProliferation2002MurineBovineMolecularEmbryoProteinsGenomeMammalian cells1988Curr BiolCharacterization19861995ChemEpithelial cellsExpressionPreimplantation mousePluripotentDifferentiationProtein2000RegulationPorcineSomaticRegulatesPhenotypesEndothelialTransgenicOocyteEncodesTarget genesTissuesMiceMutationChromosomeTranscriptionPrecursor cellsDevelopmentAdultBlastocystMutationsProgressionNuclearStem cellNeuralSubsetTranscriptional
Embryos15
- Rossant, J. & Vijh, K. M. Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. (nature.com)
- 2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
- Our study established the optimal TSA treatment on porcine donor cells and cloned embryos, 250 nmol/L, 24 h and 40 nmol/L, 24 h, respectively. (chinagene.cn)
- Aberrant methylation of donor genome in cloned bovine embryos. (chinagene.cn)
- Precise recapitulation of methylation change in early cloned embryos. (chinagene.cn)
- True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)
- The efficiency of cloning, defined as the proportion of transferred embryos that result in viable offspring, is approximately 2 to 3% for all species. (sibi.org)
- The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
- Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
- Adenot P.G., Mercier Y., Renard J.P., Thompson E.M., Differential H4 acetylation of paternal, maternal chromatin precedes DNA replication, and differential transcriptional activity in pronuclei of 1-cell mouse embryos. (biologie-journal.org)
- Bellier S., Chastant S., Adenot P., Vincent M., Renard J.P., Bensaude O., Nuclear translocation , and carboxyl-terminal domain phosphorylation of RNA polymerase II delineate the two phases of zygotic gene activation in mammalian embryos. (biologie-journal.org)
- Giraldo A.M., Hylan D.A., Ballard C.B., Purpera M.N., Vaught T.D., Lynn J.W., Godke R.A., Bondioli K.R., Effect of epigenetic modifications of donor somatic cells on the subsequent chromatin remodeling of cloned bovine embryos. (biologie-journal.org)
- Huang J.C., Lei Z.L., Shi L.H., Miao Y.L., Yang J.W., Ouyang Y.C., Sun Q.Y., Chen D.Y., Comparison of histone modifications in in vivo and in vitro fertilization mouse embryos. (biologie-journal.org)
- We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
- Influence of organochlorine pesticides on development of mouse embryos in vitro . (cdc.gov)
Reprod7
- Biol Reprod , 2005, 72(2): 309-315. (chinagene.cn)
- Biol Reprod , 2006, 75(2): 226-230. (chinagene.cn)
- Reprod Fertil Dev , 2007, 19(2): 403-423. (chinagene.cn)
- Mol Reprod Dev , 2003, 66(1): 32-37. (chinagene.cn)
- Biol Reprod 1986;35(4):799-805. (abvpress.ru)
- Biol Reprod 62:571-578. (cdc.gov)
- Biol Reprod 50(Suppl. (cdc.gov)
Vivo11
- Rossant, J. & Lis, W. T. Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo . (nature.com)
- Transcription factor CHF1/Hey2 regulates neointimal formation in vivo and vascular smooth muscle proliferation and migration in vitro. (jax.org)
- Resolution of these problems has been approached in Drosophila by carrying out an in vivo functional analysis of Drosophila Gap1 in the context of cone cell determination in the developing Drosophila eye. (sdbonline.org)
- The extent of lactogenic differentiation of HC11 cells in vitro is limited compared to mammary epithelium undergoing secretory differentiation in vivo. (biomedcentral.com)
- Downregulation of AP-2 transcription factor genes is not required for lactogenic differentiation of HC11 cells but may functionally be involved in aspects of lactogenic differentiation in vivo that are not reflected by the HC11 system. (biomedcentral.com)
- Inhibition of prolyl 4-hydroxylase in vitro and in vivo by members of a novel series of phenanthrolinones. (embl.de)
- Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
- Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use. (bdbiosciences.com)
- Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)
- The early general expression ofdri and the similarity of DRI and homeodomain in vitro DNA-bindingspecificity compound the problem of understanding the in vivo specificityof action of these proteins. (embl.de)
- Relating in vitro to in vivo exposures with physiologically-based tissue dosimetry and tissue response models. (cdc.gov)
20012
- Curr Opin Cell Biol , 2001, 13(3): 263-273. (chinagene.cn)
- In a meeting in Washington (3 December 2001) the researcher Tanja Dominko presented the results of monkey cloning (Macacus rhesus) when she worked at the Regional Center of Research in Primates of Beaverton, Oregon (USA). (sibi.org)
Fetal4
- Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
- Hey basic helix-loop-helix transcription factors are repressors of GATA4 and GATA6 and restrict expression of the GATA target gene ANF in fetal hearts. (jax.org)
- Production of transgenic cloned piglets from genetically transformed fetal fibroblasts selected by green fluorescent protein. (chinagene.cn)
- 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
Proliferation11
- Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
- Effects of chloroplast DNA content on the cell proliferation and aging in Chlamydomonas reinhardtii. (bio.net)
- Only later, the epidermis exhibits enhanced cell proliferation. (biologists.com)
- Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
- Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)
- However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
- Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
- A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
- The proliferation and differentiation of adult stem cells must be tightly balanced to avoid excessive proliferation or premature differentiation. (sdbonline.org)
- However, how stem cell proliferation is properly controlled remains elusive. (sdbonline.org)
- This study found that auxilin (Aux) restricts intestinal stem cell (ISC) proliferation mainly through EGFR signaling. (sdbonline.org)
20021
- 2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
Murine2
- We tested administration of a monoclonal antibody that enforces the active ß1 integrin conformation, (clone 9EG7), on a murine neonatal CCM mouse model, Krit1flox/flox ;Pdgfb-iCreERT2 (Krit1ECKO ), and on KRIT1-silenced human umbilical vein endothelial cells (HUVECs). (bvsalud.org)
- The undifferentiated murine neuroblastoma Neuro2a cell line (ATCC) was stably transfected with AhR cDNA and the established cell line was named N2a-Rα. (biomedcentral.com)
Bovine3
- Couldrey C., Lee R.S., DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming. (biologie-journal.org)
- Kopecny V., Fléchon J.E., Camous S., Fulka J. Jr., Nucleologenesis , and the onset of transcription in the eight-cell bovine embryo: fine-structural autoradiographic study. (biologie-journal.org)
- 1998. Influence of organochlorine pesticides on maturation and post-fertilization development of bovine oocytes in vitro . (cdc.gov)
Molecular12
- Molecular characterization and gene expression of lhcb5 gene encoding CP26 in the light-harvesting complex II of Chlamydomonas reinhardtii. (bio.net)
- Molecular Cell, 12 (3). (cshl.edu)
- Molecular Biology of the Cell, 13 (3). (cshl.edu)
- Molecular Cell, 7 (3). (cshl.edu)
- When investigating the causes of this failure via cellular and molecular analysis of 2-cell zygotes and the successive cell divisions (blastomeres), all kinds of abnormalities were found. (sibi.org)
- Extensive evidence has revealed that the core clock machinery involves "clock genes" and "clock proteins" functioning as molecular cogs. (bioone.org)
- Identification of the Menkes gene by positional cloning has enabled molecular diagnosis of females who carry the gene and at-risk fetuses in certain families, enhancing preventive efforts. (medscape.com)
- The long-term goals of the Stottmann Lab are to understand the genes and molecular mechanisms guiding normal development of both the brain and face. (nationwidechildrens.org)
- They use a combination of human genetics and animal models to identify genes required for normal development and then study the molecular consequences of disrupting their function in the developing embryo. (nationwidechildrens.org)
- but the molecular mechanisms underlying how cells adapt to variations in oxygen supply were unknown until the prize-winning work described here. (nobelprize.org)
- The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
- 7. Swinnen J.V., Joseph D.R., Conti M. Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes. (abvpress.ru)
Embryo15
- The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
- Louvet-Vallee, S., Vinot, S. & Maro, B. Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. (nature.com)
- Furthermore, we found that both the cloned embryo and the donor cell treated by TSA resulted in the highest development efficiency. (chinagene.cn)
- Meanwhile, TSA can improve transgene expression in donor cell and cloned embryo. (chinagene.cn)
- TSA improve transgenic porcine cloned embryo development and transgene expression[J]. HEREDITAS, 2011, 33(7): 749-756. (chinagene.cn)
- Among the factors thought to contribute to the greater success in cloning cattle are the relatively late embryonic genome activation specific for this species [16 -18] and the optimization of reproductive technologies, such as in vitro embryo production and embryo transfer, brought about by the cattle industry [19]. (sibi.org)
- Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
- Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
- 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
- Up to the 2-cell embryo, blastomeres remain totipotent (Garner and McLaren, 1974). (conditionmed.org)
- In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
- Adenot P.G., Szollosi M.S., Geze M., Renard J.P., Debey P., Dynamics of paternal chromatin changes in live one-cell mouse embryo after natural fertilization. (biologie-journal.org)
- Aoki F., Worrad D.M., Schultz R.M., Regulation of transcriptional activity during the first , and second cell cycles in the preimplantation mouse embryo. (biologie-journal.org)
- Bouniol C., Nguyen E., Debey P., Endogenous transcription occurs at the 1-cell stage in the mouse embryo. (biologie-journal.org)
- Maternally derived dri product is foundthroughout the embryo until germ band extension, when dri is expressed ina developmentally regulated set of tissues, including salivary glandducts, parts of the gut, and a subset of neural cells. (embl.de)
Proteins7
- To determine how the ASAP complex interacts with the EJC core, we performed in vitro coprecipitation assays 3 with Tandem Affinity Purification (TAP)-tagged recombinant proteins corresponding to full-length human SAP18, RNPS1 and ACINUS. (nature.com)
- LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
- Adipocyte-derived factors involved in tumor progression include proteins such as adiponectin, leptin, TNF-α, monocyte chemotactic protein-1 (MCP-1), IL-6, and ECM components that control tumor cell behavior within the tumor microenvironment. (jci.org)
- Disrupting any component in this pathway led to disrupted SD on the cell surface and intracellular accumulation of mislocalized SD proteins. (sdbonline.org)
- All genes in this SD protein endocytosis and recycling pathway, as well as SD proteins themselves, are highly conserved from flies to humans. (sdbonline.org)
- Thus, these results suggest that the SD proteins in human kidney undergo the same endocytosis and recycling pathway to maintain the filtration structure, and mutations in any genes in this pathway could lead to abnormal SD and renal diseases. (sdbonline.org)
- Characterization of the dead ringer gene identifies a novel, highlyconserved family of sequence-specific DNA-binding proteins. (embl.de)
Genome7
- First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
- The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
- Following germinal vesicle break down (GVBD) the oocyte is transcriptionally quiescent until fertilization and activation of the embryonic genome, occurring at the four-cell stage of development in the pig [ 9 ]. (biomedcentral.com)
- Telomeres are specialized structures at the end of chromosomes essential for maintaining genome stability and cell viability. (biomedcentral.com)
- Telomeres are essential for maintaining genome stability and cell viability, with dysfunctional telomeres triggering a classic DNA-damage response that enables double-strand breaks and cell cycle arrest [ 6 ]. (biomedcentral.com)
- Cyclin-G-associated kinase (GAK) , a PD susceptibility gene identified through genome-wide association studies (GWAS), is a ubiquitous serine/threonine kinase involved in clathrin uncoating (see Drosophila Clathrin heavy chain ), though its PD-related function remains elusive. (sdbonline.org)
- possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)
Mammalian cells1
- Although BK virus, JC virus, and simian virus 40 are tumorigenic in experimental animals and can transform mammalian cells in vitro, no convincing epidemiologic evidence exists for their role in human cancers. (cdc.gov)
19884
- Cell 54 , 83-93 (1988). (nature.com)
- Cell 54 , 95-104 (1988). (nature.com)
- 1988) Gene 67: 21-30), glutathione-S-transferase (Johnson (1989) Nature 338: 585-587), ubiquitin (Miller et al. (justia.com)
- Mech Ageing Dev 1988;43(1):61-9. (abvpress.ru)
Curr Biol1
- Curr Biol. (jax.org)
Characterization5
- Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
- Characterization of the human and mouse HEY1, HEY2, and HEYL genes: cloning, mapping, and mutation screening of a new bHLH gene family. (jax.org)
- Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)
- Dr. Multani has extensive expertise in the field of Human and Mammalian Cytogenetics, and specializes in the evaluation of genetic instability and complex chromosomal rearrangements in cancer cells, telomere dysfunction, characterization of embryonic stem cells, and authentication of cell lines. (mdanderson.org)
- We reported the identification of a new family of DNA-binding proteinsfrom our characterization of the dead ringer (dri) gene of Drosophilamelanogaster. (embl.de)
19861
- In 1986, the NF2 gene was mapped to chromosome 22, and in 1993, two groups independently isolated the gene and named its encoding protein merlin or schwannomin. (bmj.com)
19952
- In studies during the early 1990's, Gregg Semenza identified, and then in 1995 purified and cloned, a transcription factor that regulates these oxygen-dependent responses. (nobelprize.org)
- William Kaelin, Jr. was in 1995 engaged in the study of the von Hippel-Lindau tumor suppressor gene, and after isolation of the first full-length clone of the gene showed that it could suppress tumor growth in VHL mutant tumorigenic cell lines. (nobelprize.org)
Chem1
- J Biol Chem. (jax.org)
Epithelial cells6
- Secretory epithelial cells display lactogenic differentiation which is characterized by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
- To explore whether the downregulation of AP-2alpha and AP-2gamma is of functional significance for lactogenic differentiation, we analyzed the expression of the AP-2 family members during the lactogenic differentiation of HC11 mammary epithelial cells in vitro. (biomedcentral.com)
- The lactogenic differentiation of the secretory epithelial cells is characterized by the synthesis of lactose and milk fat and by the expression of milk protein genes, such as beta-casein or whey acidic protein (WAP). (biomedcentral.com)
- This mechanism preserves the single-cell layer pattern throughout the body and prevents epithelial cells from growing in inappropriate locations. (elifesciences.org)
- explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
- Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)
Expression35
- Furthermore, mutation in the LRPPRC gene causes lowered expression of MT-CO1 (cytochrome c oxidase I) and MT-CO3. (wikipedia.org)
- This study explores the use of a simple human neurosphere-based in vitro model to characterise the pharmacological and toxicological effects of LiCl and VPA using gene expression changes linked to phenotypic alterations in cells. (plos.org)
- Treatment with VPA and LiCl resulted in the differential expression of 331 and 164 genes respectively. (plos.org)
- In addition, in order to phenotypically anchor the gene expression data, changes in the heterogeneity of cell subtype populations and cell cycle phase were monitored using flow cytometry. (plos.org)
- Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. (nature.com)
- Follow the symbol links to get more information on the GO terms, expression assays, orthologs, phenotypic alleles, and other information for the genes or markers below. (jax.org)
- CHF1/Hey2 plays a pivotal role in left ventricular maturation through suppression of ectopic atrial gene expression. (jax.org)
- The exon junction complex (EJC) deposited on spliced mRNAs, plays a central role in the post-transcriptional gene regulation and specific gene expression. (nature.com)
- The Exon Junction Complex (EJC) plays a central role in post-transcriptional gene expression control. (nature.com)
- These observations strongly suggest the implication of EJC in fine-tuning of specific gene expression in different cellular contexts. (nature.com)
- We compared microRNA expression patterns in three cisplatin resistant sublines derived from paternal cisplatin sensitive germ cell tumor cell lines in order to improve our understanding of the mechanisms of cisplatin resistance. (biomedcentral.com)
- Production of the fusion polypeptide involves the introduction of the hybrid gene into a biological host cell system, for example yeast cells, which permits the expression and accumulation of the fusion polypeptide. (justia.com)
- We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
- We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)
- To introduce altered Drosophila Gap1 transgenes into flies, the sevenless ( sev ) enhancer/promoter cassette, an eye-specific promoter, was used to drive expression in cone cell precursors at appropriate levels. (sdbonline.org)
- Expression of one copy of wild type Gap1 under control of the sev enhancer/ promoter cassette ( sevGap1 ) rescues the complete loss-of-function eye phenotype, while creating no phenotypic defects when placed in a wild type background containing two copies of the endogenous Gap1 gene. (sdbonline.org)
- Cloning, expression and biological activity of a new variant of human interferon alpha identified in virus induced lymphoblastoid cells. (nih.gov)
- Differentiating HC11 cells displayed lower expression levels of milk protein genes than mammary glands of mid-pregnant or lactating mice. (biomedcentral.com)
- They share a conserved structure and control gene expression by binding to the respective promoters as homo- or heterodimers. (biomedcentral.com)
- In this report we analyzed AP-2 transcription factor expression in HC11 cells subjected to lactogenic differentiation conditions. (biomedcentral.com)
- Their failures or incompletion (regulation and gene expression, epigenetic, etc.) are attributed to the inviability and losses detected in the laboratory as well as the pathologies observed during the animal´s pregnancy and offspring after birth or later. (sibi.org)
- Designed PTX3‐siRNAs ( n =53) were assessed and scored from different aspects and the best one was suggested in PTX3 gene expression knockdown assay. (ac.ir)
- SATB1 can recruit chromatin-remodeling factors and thereby regulate chromatin structure and gene expression. (bdbiosciences.com)
- Alvarez JD, Yasui DH, Niida H, Joh T, Loh DY, Kohwi-Shigematsu T. The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development. (bdbiosciences.com)
- Animal cells undergo fundamental shifts in gene expression when there are changes in the oxygen levels around them. (nobelprize.org)
- These changes in gene expression alter cell metabolism, tissue re-modeling, and even organismal responses such as increases in heart rate and ventilation. (nobelprize.org)
- Through the combined work of these three laureates it was thus demonstrated that the response by gene expression to changes in oxygen is directly coupled to oxygen levels in the animal cell, allowing immediate cellular responses to occur to oxygenation through the action of the HIF transcription factor. (nobelprize.org)
- However, for most of the 20th century, it was not clear how adaptations to oxygen flux were regulated at the fundamental level of gene expression. (nobelprize.org)
- Braude P., Bolton V., Moore S., Human gene expression first occurs between the four- , and eight-cell stages of preimplantation development. (biologie-journal.org)
- The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
- Morphologically, N2a-Rα cells bore spontaneous neurites exhibiting axon-like properties with the localization of NF-H. In addition, cdc42 expression was increased in comparison to the control cell line. (biomedcentral.com)
- BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
- Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
- Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
- 1 In particular, morpholino oligonucleotides (MOs) have been used to block the expression of targeted genes in several invertebrate and vertebrate models. (cdc.gov)
Preimplantation mouse1
- Graham, C. F. & Lehtonen, E. Formation and consequences of cell patterns in preimplantation mouse development. (nature.com)
Pluripotent6
- Human embryonic stem cells (hESc) can also be used to analyze the in vitro development from undifferentiated pluripotent cells leading to terminally differentiated cell types, recapitulating the process of early embryonic development [5] . (plos.org)
- The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)
- Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
- Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
- Prior to SCNT, the somatic cell (differentiated) must be reprogramed to a similar state of a pluripotent embryonic cell (undifferentiated) before the nucleus is extracted and transferred. (sibi.org)
- Katherine studies a gene that causes microcephaly and cerebellar hypoplasia in human pediatric patients, using mice and human induced pluripotent stem cells to reveal why this gene is influential for brain development. (nationwidechildrens.org)
Differentiation13
- 2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
- The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
- Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
- Differentiation of HC11 cells was induced following established protocols by applying the lactogenic hormones prolactin, dexamethasone and insulin. (biomedcentral.com)
- Using RT-PCR we could not detect a downregulation of any of these genes during the lactogenic differentiation of HC11 cells in vitro. (biomedcentral.com)
- To these ends, an in vitro system of lactogenic differentiation would be informative. (biomedcentral.com)
- HC11 cells can be induced to undergo lactogenic differentiation by applying a mixture of the lactogenic hormones dexamethasone, insulin and prolactin (a mixture termed DIP in the following sections). (biomedcentral.com)
- For induction of differentiation, cells were grown to confluency and then kept in medium without EGF for 48 hours to induce competence. (biomedcentral.com)
- It demonstrated that genes inactivated during tissue differentiation can be completely re-activated by a process called nuclear reprogramming: the reversion of a differentiated nucleus back to a totipotent status. (biomedcentral.com)
- To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
- Thus, exogenous AhR induced catecholaminergic differentiation in N2a-Rα cells. (biomedcentral.com)
- The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)
- Activated AhR may disrupt the strictly regulated brain formation with irregular differentiation occurring rather than cell death. (biomedcentral.com)
Protein11
- Leucine-rich PPR motif-containing protein, mitochondrial is a protein that in humans is encoded by the LRPPRC gene. (wikipedia.org)
- This gene encodes a protein that is leucine-rich and is thought to play a role in regulating the interaction of the cytoskeleton with a variety of cellular processes. (wikipedia.org)
- Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
- This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)
- Using a fluorescent sulfhydryl probe as well as a sulfhydryl inhibitor, one observes a strong cell density response, supporting the hypothesis that cellular redox potential could alter protein stability. (embl.de)
- Thus, the maturing oocyte is reliant on processes such as post-transcriptional gene regulation (PTGR) to regulate the mRNA and protein repertoire. (biomedcentral.com)
- The 14/SATB1 monoclonal antibody specifically binds to the special AT-rich sequence binding protein 1 that is encoded by the SATB1 (SATB homeobox 1) gene. (bdbiosciences.com)
- They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
- also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)
- Acanthamoeba binding to HBMEC is mediated by a mannose-binding protein expressed on the surface of Acanthamoeba cells ( 2 ). (asm.org)
- Using the Drosophila nephrocyte as a genetic screen platform, most genes involved in endocytosis and cell trafficking were screened, and the key components were identified of the cell trafficking pathway required for SD protein endocytosis and recycling. (sdbonline.org)
20002
- 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
- 2000. A metabolite of methoxychlor, 2,2-bis( p hydroxyphenyl)-1,1,1-trichloroethane, reduces testosterone biosynthesis in rat leydig cells through suppression of steady-state messenger ribonucleic acid levels of the cholesterol side-chain cleavage enzyme. (cdc.gov)
Regulation5
- This study provides the first evidence that different EJCs can have distinct functions, illuminating EJC-dependent gene regulation. (nature.com)
- Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
- Alternative sites for cell density regulation of the enzyme could rely on its sensitivity to sulfhydryl groups. (embl.de)
- The inability to transcribe mRNA during this stage of development and the probable necessity for post-transcriptional gene regulation (PTGR) suggests an important role for non-coding RNA in the maturing oocyte. (biomedcentral.com)
- ARID-encoding genes are involved in a variety of biological processes including embryonic development, cell lineage gene regulation and cell cycle control. (embl.de)
Porcine2
- 3] Porcine islet cells of Langerhans have been injected into patients with type 1 diabetes mellitus. (medscape.com)
- 4] Porcine skin has been grafted onto burn patients,[5] and pig neuronal cells have been transplanted into patients with Parkinson (Parkinson's) disease and Huntington (Huntington's) disease. (medscape.com)
Somatic14
- Trichostatin A (TSA), as an inhibitor of histone deacetylase, can change the status of histone acetylation, improve somatic cell reprogramming, and enhance cloning efficiency. (chinagene.cn)
- 1] Vajta G. Somatic cell nuclear transfer in its first and sec-ond decades: successes, setbacks, paradoxes and perspectives. (chinagene.cn)
- 6] Vajta G, Zhang YH, Macháty Z. Somatic cell nuclear transfer in pigs: recent achievements and future possibilities. (chinagene.cn)
- Twenty years have passed since Dolly the sheep was born by cloning (somatic cell nuclear transfer, SCNT) but the results of non-human mammalian cloning are very poor, and cause animal diseases and huge biological losses. (sibi.org)
- So far the reprogramming of somatic cells shows very low rates of efficiency (~0.0006-1%) that have not improved in the last two decades of continuous research. (sibi.org)
- I believe that the reprogramming errors are not the only cause of these low rates of cloning: the mammalian SCNT fails with a very high frequency mainly due to the damage that the technique itself inflicts in the egg and the somatic nucleus, and the very few successful cases occur only when the damage is not significant. (sibi.org)
- Despite immense promise, somatic cell reprogramming still faces a critical challenge. (sibi.org)
- Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
- However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)
- These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
- Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
- Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
- Schematic diagram of the somatic cloning process. (biomedcentral.com)
- Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
Regulates3
- Cell density regulates prolyl 4-hydroxylase activity independent of mRNA levels. (embl.de)
- In embryonic avian tendon, cell density regulates collagen production. (embl.de)
- AhR regulates the transcription of diverse genes through binding to the xenobiotic-responsive element (XRE). (biomedcentral.com)
Phenotypes1
- Chromosomal abnormalities, HLA phenotypes, and polymorphisms of various genes have been associated with acne. (springer.com)
Endothelial10
- ß1 integrins are important in blood vessel formation and function, finely tuning the adhesion of endothelial cells to each other and to the extracellular matrix. (bvsalud.org)
- Endothelial loss of the gene KRIT1 leads to brain microvascular defects, resulting in debilitating and often fatal consequences. (bvsalud.org)
- Treatment with 9EG7 reduced lesion burden in the Krit1ECKO model and was accompanied by a strong reduction in the phosphorylation of the ROCK substrate, myosin light chain (pMLC), in both retina and brain endothelial cells. (bvsalud.org)
- ETP augmented fibrosis, angiogenesis, and inflammation through recruitment of macrophages and endothelial cells. (jci.org)
- We studied the effects of an Acanthamoeba encephalitis isolate belonging to the T1 genotype on human brain microvascular endothelial cells, which constitute the blood-brain barrier. (asm.org)
- Using an apoptosis-specific enzyme-linked immunosorbent assay, we showed that Acanthamoeba induces programmed cell death in brain microvascular endothelial cells. (asm.org)
- Acanthamoeba -mediated brain endothelial cell death was abolished using LY294002, a phosphatidylinositol 3-kinase inhibitor. (asm.org)
- These results were further confirmed using brain microvascular endothelial cells expressing dominant negative forms of phosphatidylinositol 3-kinase. (asm.org)
- This is the first demonstration that Acanthamoeba -mediated brain microvascular endothelial cell death is dependent on phosphatidylinositol 3-kinase. (asm.org)
- We have demonstrated that pathogenic Acanthamoeba exhibits more than 60% binding to human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier ( 2 ). (asm.org)
Transgenic4
- Uncompleted epigenetic reprogramming is attributed to the low efficiency of producing transgenic cloned animals. (chinagene.cn)
- Cloned transgenic swine via in vitro production and cryopreservation. (chinagene.cn)
- In combination with transgenic organisms, such enzyme-actuated antisense tools will enable gene silencing in specific cell types, including tissues that are not amenable to optical targeting. (cdc.gov)
- 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
Oocyte2
- Maturation of the mammalian oocyte is a complex process involving internal checkpoints and bidirectional communication with the surrounding cumulus cells. (biomedcentral.com)
- A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Encodes2
- The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)
- Evidence that the gene encodes a highly conserved copper-transporting adenosine triphosphatase (ATPase) has stimulated investigation of the molecule's normal function in prokaryotic and eukaryotic systems. (medscape.com)
Target genes2
Tissues6
- Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
- The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)
- A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
- Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
- This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
- Tissues and Cells. (lu.se)
Mice2
- Oligozoospermia with normal fertility in male mice lacking the androgen receptor in testis peritubular myoid cells. (abvpress.ru)
- The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Mutation4
- The GTPase signalling molecules RhoA and Rac1 regulate merlin function, but to date only mutation in the NF2 gene has been identified as a causal event in schwannoma formation. (bmj.com)
- 5, 6 The NF2 gene is a tumour suppressor, as corroborated by studies showing complete inactivation of the gene in more than 60% of schwannomas, either by mutation in both alleles or by loss of one allele and mutation in the other. (bmj.com)
- cell detects it by means of the DNA result in a mutation. (who.int)
- Other types repair the damage but instead pro- mutagens produce DNA damage, of DNA damage are oxidized or frag- cess it into a mutation, or direct the and they might more appropriately mented bases and the intercalation cell to undergo apoptosis. (who.int)
Chromosome4
- The NF2 gene is a tumour suppressor on chromosome 22. (bmj.com)
- Comparative genomic hybridisation (CGH) was used to screen 76 vestibular schwannomas from 76 patients (66 sporadic and 10 NF2 related) to identify other chromosome regions that may harbour genes involved in the tumorigenesis. (bmj.com)
- Nucleotide sequence of a portion of human chromosome 9 containing a leukocyte interferon gene cluster. (nih.gov)
- These complexes, which are abundant at chromosome ends but do not accumulate elsewhere, are present at telomeres throughout the cell cycle and their action is limited to telomeres [ 7 , 8 ]. (biomedcentral.com)
Transcription1
- Canonical interleukin-2 (IL-2) signaling via the high-affinity CD25-containing IL-2 receptor-Janus kinase (JAK)1,3-signal transducer and activator of transcription 5 (STAT5) pathway is essential for development and maintenance of CD4+CD25HiFoxp3+ regulatory T cells (Tregs) that support immune homeostasis. (bvsalud.org)
Precursor cells2
- Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
- The basic helix-loop-helix genes Hesr1/Hey1 and Hesr2/Hey2 regulate maintenance of neural precursor cells in the brain. (jax.org)
Development4
- Genes & Development, 16 (20). (cshl.edu)
- The overexpression of PTX3 gene is profoundly implicated in glioma development. (ac.ir)
- 2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
- It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
Adult1
- Adult tissue homeostasis is maintained by residential stem cells. (sdbonline.org)
Blastocyst4
- Fleming, T. P. A quantitative analysis of cell allocation to trophectoderm and inner cell mass in the mouse blastocyst. (nature.com)
- The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)
- We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
- The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
Mutations2
- For practical purposes, depending on the enzyme activity and the presence of mutations in the G6Pase and T genes, respectively, GSD type I may be subdivided into 2 major forms. (medscape.com)
- It is an autosomal recessive disorder in which there is an AGL gene mutations which causes deficiency in glycogen debranchinging enzyme and limited storage of dextrin. (medscape.com)
Progression3
- The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
- Pentraxin 3 promotes glioblastoma progression by negative regulating cells autophagy. (ac.ir)
- During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)
Nuclear2
- Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
- Francastel C., Magis W., Groudine M., Nuclear relocation of a transactivator subunit precedes target gene activation. (biologie-journal.org)
Stem cell2
- Stem Cell Res. (sciendo.com)
- Stem Cell Reports 17(5): 1120-1137. (sdbonline.org)
Neural1
- CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
Subset3
- In the subset of VPA targeted genes, 114 were downregulated whilst 217 genes were upregulated. (plos.org)
- In the subset of LiCl targeted genes, 73 were downregulated and 91 were upregulated. (plos.org)
- Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
Transcriptional2
- Using the bioinformatics tool MAGICTRICKS and reversible pharmacological inhibition, we found that primed genes required the transcriptional activities of NF-κB. (frontiersin.org)
- Recently it has been reported that repression of SATB1 was essential for the phenotype and function of mouse regulatory T cells but inhibitory for the establishment of transcriptional programs expressed by effector T cells. (bdbiosciences.com)