• In vivo, DIDS administration delayed B16OVA tumor growth in immunocompetent mice as monotherapy or when combined with adoptive T cell transfer of OVA-specificT cells. (unav.edu)
  • Notably, genetic Ae2 silencing in OVA-specificT cells improvedCD4(+)/CD8(+) T cell function in vitro as well as their antitumor activity in vivo. (unav.edu)
  • Secreted FKN inhibits lung adenocarcinoma growth in vivo through a prominent contribution of systemic effector NK cells and increased tumor immune infiltration. (bvsalud.org)
  • Scientifically speaking, organoids are in vitro generated three-dimensional (3D), mini-cluster of cells that highly simulate the structure and function of the corresponding organ in vivo [1] . (danabiosci.com)
  • The improvement of cell culture conditions through mimicking the in vivo microenvironment was made in 1987. (danabiosci.com)
  • The immune system which results from cells of the lymphoid and myeloid lineages is developed in vivo, so as to recognize self from non-self. (justia.com)
  • Moreover, in vivo , the proliferation of the tumor cells was evaluated in xenotransplant nude mice model with whole-body visualizing instrument. (biomedcentral.com)
  • In that same study, we observed that FE65 increased sApoER2 and ApoER2 CTF in COS7 cells, whilst knockdown of FE65 brought on decreased ApoER2 CTF in vivo. (srcpathway.com)
  • Dr. Abdelbaset-Ismail has developed remarkable technical skills in cell transplantation for various animal models, molecular characterization, propagating cells in vitro and in vivo. (livedna.net)
  • Indeed, exposing aggregated P19 cells to dimethyl sulfoxide (DMSO) induces differentiation into cardiac and skeletal muscle. (wikipedia.org)
  • By aggregating the cells into an embryonic body, EC cells can also process differentiation. (wikipedia.org)
  • In P19 cells, addition of non-toxic concentrations of drugs to aggregated embryoid body cells can induce differentiation of P19 cells into specific cell lines depending on the added drug. (wikipedia.org)
  • Because the concentration of retinoic acid or DMSO is nontoxic to the cells, the drug-specific differentiation is due to induction of cells not selection. (wikipedia.org)
  • Mutants of P19 cells have been generated to investigate the mechanism of drug-specific differentiation. (wikipedia.org)
  • The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Differentiation of embryoid bodies in vitro was observed in 1961 by Pierce and Verney [6] . (danabiosci.com)
  • discovered the differentiation of alveolar type II epithelial cells in ECM extract [10] . (danabiosci.com)
  • The hsa-miR-371-373 cluster (counteracting cellular senescence and linked with differentiation potency), as well as hsa-miR-520c/-520h (inhibiting the tumor suppressor p21) were 3.9-16.3 fold up-regulated in two of the three cisplatin resistant cell lines. (biomedcentral.com)
  • It is widely used in biomedical research, mainly for studying developmental biology, stem cell biology, cell differentiation, and drug screening. (cytion.com)
  • As P19 cells have differentiation ability, they can be useful in investigating complex biological processes such as tissue formation and early embryonic development. (cytion.com)
  • Thus, P19 cells can be useful in studying cell differentiation and developmental processes. (cytion.com)
  • In this review, we describe the cardiac differentiation from ES cells, iPS cells, and the current progress of using iPS cell-derived cardiomyocytes for heart disease modeling and for the development of therapeutic strategies. (biomedcentral.com)
  • The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
  • The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
  • Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity. (springer.com)
  • Role of Otx2 in Embryonic Stem Cells (ESCs): Otx2 is an intrinsic determinant required to maintain the ESC metastable state by antagonizing ground state pluripotency and promoting commitment to differentiation. (cnr.it)
  • Here, we present a quantitative and comprehensive study of the abundance levels of histone PTMs during the differentiation of mouse embryonic stem cells (ESCs) using mass spectrometry (MS). We observed dynamic changes of histone PTMs including increased H3K9 methylation levels in agreement with previously reported results. (biomedcentral.com)
  • Together, our data demonstrate the fundamental role of Brd4 in monitoring cell differentiation through its interaction with acetylated histone marks and disruption of Brd4 may cause aberrant differentiation. (biomedcentral.com)
  • For his doctoral thesis, Dr. Brinster developed the first reliable in vitro culture system for early mammalian embryos. (avma.org)
  • Today, this technique continues to form the foundation for research on mammalian embryos, including technologies such as transgenic engineering, embryonic stem cell therapy, human in vitro fertilization, mammalian cloning, and knockout engineering. (avma.org)
  • From there, Dr. Brinster became interested in modifying the development of animals and their germ lines, and he went on to become the first person to show that it was possible to colonize a mouse blastocyst with stem cells from older embryos. (avma.org)
  • Pluripotent stem cells (PSCs) were first isolated and established from mouse embryos in 1981 [7] [8] . (danabiosci.com)
  • 1981 and 1988 were important years in the history of organoids because in these years pluripotent stem cells from mouse embryos and embryonic stem cells derived from human blastocysts were isolated and cultured for the very first time (3). (facellitate.com)
  • 3. Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. (facellitate.com)
  • Embryonic stem (ES) cells are naturally derived from early stage embryos and induced pluripotent stem (iPS) cells are reprogrammed from somatic cells with overexpression of four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc. (biomedcentral.com)
  • Establishment in culture of pluripotential cells from mouse embryos. (springer.com)
  • Stem cells may be derived from adult tissues but the most potent are extracted from developing human embryos. (edu.au)
  • In 2009, Hans Clevers and colleagues first demonstrated that single Lgr5 + -expressing intestinal adult stem cells (ASCs) could self-organize and differentiate to form intestinal crypt-villus structures that contained all intestinal cell types [14] . (danabiosci.com)
  • An important breakthrough happened when it was reported that the adult intestinal stem cells expressing the single leucine-rich repeat-containing G protein-coupled receptor 5 (Lgr5) can form 3D intestinal organoids in Matrigel-containing media and can self-organize and differentiate into crypt-villus structures (4). (facellitate.com)
  • Forkhead box P3-peptide inhibitor P60 has been shown to specifically inhibit regulatory T-cell function in murine models. (bvsalud.org)
  • Aim of this study was to investigate whether P60 can improve the immune response induced by vaccination with adenovirus-transduced dendritic cells expressing alpha-fetoprotein in subcutaneous and orthotopic murine models for hepatocellular carcinoma. (bvsalud.org)
  • Foxp3 inhibition with P60 enhanced chemosensitivity and reduced cell survival and migration in human and murine breast tumor cells. (bvsalud.org)
  • High-throughput profiling of soluble factors in plasma identifies fractalkine (FKN), a chemokine involved in immune chemotaxis and adhesion, as a biomarker of response to immunotherapy that also correlates with myeloid cell diversity in human patients and murine models. (bvsalud.org)
  • In 2010, renal organoids were produced from murine fetus-derived kidney stem cells [15] . (danabiosci.com)
  • No immunoreactivity is seen with murine EC, EG or ES cells. (sigmaaldrich.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • P19 is a murine embryonal carcinoma cell line. (cytion.com)
  • NCCIT cells (ATCC CRL-2073) were stained with either PE Mouse IgG1, κ Isotype Control (Cat. (bdbiosciences.com)
  • Three cisplatin resistant sublines (NTERA-2-R, NCCIT-R, 2102EP-R) showing 2.7-11.3-fold increase in drug resistance after intermittent exposure to increasing doses of cisplatin were compared to their parental counterparts, three well established relatively cisplatin sensitive germ cell tumor cell lines (NTERA-2, NCCIT, 2102EP). (biomedcentral.com)
  • The paternal cell lines are (1) the p53-wt (wild type) pluripotent gonadal germ cell tumor cell line NTERA-2, (2) the p53-mutated pluripotent extragonadal nonseminomatous germ cell tumor cell line NCCIT and (3) the p53-wt nullipotent embryonal cell carcinoma 2102EP, derived from a primary human testicular teratocarcinoma. (biomedcentral.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • Degradation of the extracellular matrix allows tumor cells to detach from the primary tumor mass, invade local tissue, intravasate, extravasate and build new metastatic formations [ 1 ]. (biomedcentral.com)
  • More colloquially, an organoid is a mini-organ that is generated in vitro with the ability to self-renew and self-organize and performs organ functions similar to those of the tissue of origin. (danabiosci.com)
  • Organoids are self-organizing culture systems generated from stem cells or tissue-specific progenitor cells. (facellitate.com)
  • Stem cells divide indefinitely and produce different types of cells replicating the complexity of a tissue or an organ. (facellitate.com)
  • Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
  • To do so, cells release signaling molecules that can move around within a tissue and be detected by receptors on other cells. (elifesciences.org)
  • We tend to assume that the signaling molecules are evenly distributed across a tissue and affect all the receiving cells in the same way. (elifesciences.org)
  • Cells responding to the signal somehow can ignore this noise and establish sharp boundaries between different cell types so that neighboring cells have distinct roles in the tissue. (elifesciences.org)
  • He has also gained salient experience in various experimental setups using hematopoietic stem cells, bone marrow, cord blood-derived and adipose tissue-derived MSCs, very small embryonic-like stem cells, leukemic cells, and germline-derived cells. (livedna.net)
  • In addition, embryonic stem cells may be modified by homologous recombination for use in producing chimeric or transgenic mammalian hosts, which may be used as source of universal donor organs. (justia.com)
  • The protective mechanism which protects the host from disease, as a result of invasion of viruses, bacteria, or other pathogens, is also able to recognize cells which come from a different mammalian host, even an allogeneic host. (justia.com)
  • Viable offspring derived from fetal and adult mammalian cells. (springer.com)
  • In mammalian somatic cells, there are four major lamins, A, B1, B2, and C. The B-type lamins are encoded by separate genes (LMNB1 and LMNB2) and, as a class, are found in the nuclei of all mammalian somatic cells. (rupress.org)
  • Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). (sigmaaldrich.com)
  • Moreover, we describe for the first time an association of the up-regulation of micro-RNA species such as hsa-miR-512-3p/-515/-517/-518/-525 and down-regulation of hsa-miR-99a/-100/-145 with a cisplatin resistant phenotype in human germ cell tumors. (biomedcentral.com)
  • Our approach was designed to characterize the role of micro-RNAs on the presumably multifactorial phenomenon of acquired cisplatin resistance in germ cell tumors. (biomedcentral.com)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Histone acetylation has been implicated to be important for the pluripotent cell state, as some HDAC inhibitors have been shown to improve reprogramming efficiency of induced pluripotent stem cells (iPSCs) or maintain a pluripotent stem cell state. (biomedcentral.com)
  • The difference between these subtype cell lines is the ability to differentiate into neuronal cells or muscle cells in response to treatment with retinoic acid or DMSO, respectively. (wikipedia.org)
  • Expression analysis of the human Hox Complex in teratocarcinoma cells (N-tera2/D1) upon treatment with retinoic acid and the definition of temporal colinearity concept. (cnr.it)
  • ES cells were aggregated into three-dimensional structures, termed embryoid bodies (EBs), and suspended in media containing fetal calf serum. (biomedcentral.com)
  • P19 cells is an embryonic carcinoma cell line derived from an embryo-derived teratocarcinoma in mice. (wikipedia.org)
  • Also, it is the most characterized embryonic carcinoma (EC) cell line that can be induced into cardiac muscle cells and neuronal cells by different specific treatments. (wikipedia.org)
  • For developmental biologists, embryonal carcinoma, which is derived from teratocarcinoma, is a good object for developmental study. (wikipedia.org)
  • These stem cells were named embryonal carcinoma P19 cells. (wikipedia.org)
  • Because embryonal carcinoma can differentiate into cells of all three germ layers, P19 cells can also differentiate into those ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
  • When embryonal carcinoma cells are cultured at high density, they start to differentiate. (wikipedia.org)
  • BACKGROUND & AIMS: Vaccination with tumor-associated antigen-pulsed dendritic cells leads to specific T-cell response against hepatocellular carcinoma. (bvsalud.org)
  • High regulatory T-cell count is associated with poor prognosis and seems to mediate immune tolerance in hepatocellular carcinoma. (bvsalud.org)
  • However, P60 enhanced the antitumoral effect of a vaccination with alpha-fetoprotein-expressing dendritic cells in established subcutaneous and orthotopic hepatocellular carcinoma characterized by high Treg levels (p = .011). (bvsalud.org)
  • This emphasizes the importance of regulatory T-cells inhibition for obtaining an effective antitumoral immune response in hepatocellular carcinoma. (bvsalud.org)
  • P19 is a type of pluripotent embryonal carcinoma cells originally obtained from teratocarcinoma developed in a C3H/He mouse. (cytion.com)
  • These mouse carcinoma cells possess an epithelial-like morphology. (cytion.com)
  • P19 embryonic carcinoma cell line is adherent. (cytion.com)
  • A humidified incubator set at 37°C with a 5% CO2 supply is essential to growing and culturing the P19 embryonic carcinoma cell line. (cytion.com)
  • Pluripotent embryonic carcinoma cell line P19 is a useful model for studying early embryonic development. (cytion.com)
  • Knockdown of Cripto-1 inhibits the proliferation, migration, invasion, and angiogenesis in prostate carcinoma cells. (cusabio.com)
  • Results identified elevated CR-1 expression in esophageal squamous cell carcinoma (ESCC) specimens which correlated to poor prognosis of the patients. (cusabio.com)
  • Embryonic stem cell lines derived from human blastocysts. (springer.com)
  • Evans MD, Kelley J. US attitudes toward human embryonic stem cell research. (springer.com)
  • Embryonic stem cell technology is still at a preliminary research stage and announcements about its potential may be premature. (edu.au)
  • Experts from around the world are assessing the difficult issue of the extent to which embryonic stem cell research should be allowed to proceed, and to date there is little international consensus on this matter. (edu.au)
  • How, then, should embryonic stem cell research be regulated in Australia? (edu.au)
  • In this article we examine embryonic stem cell research and explore the current regulatory framework associated with this research in Australia, with particular reference to the Andrews Report . (edu.au)
  • Also, exposing P19 cells to retinoic acid (RA) can differentiate them into neuronal cells. (wikipedia.org)
  • Treatment of undifferentiated P19 cells with retinoic acid can specifically induce them into neuronal cells. (wikipedia.org)
  • They can be differentiated into other cell types when exposed to non-toxic drugs such as retinoic acid and dimethyl sulfoxide (DMSO) [1]. (cytion.com)
  • Retinoic acid induces the development of neurons, microglia, and astroglia, whereas DMSO initiates beating cardiomyocytes and smooth muscle cell development from P19 cells. (cytion.com)
  • Two cells in the same position can have very different retinoic acid levels, and the levels in a particular cell can vary from one minute to the next. (elifesciences.org)
  • The experiments also show that proteins that interact with retinoic acid help to reduce noise within a cell. (elifesciences.org)
  • The cell line is pluripotent and can differentiate into cell types of all three germ layers. (wikipedia.org)
  • Studies have shown that a certain concentration of RA can induce P19 cells to differentiate into neuronal cells, including neurons and glial cells, whereas 0.5% - 1% DMSO led P19 cells to differentiate into cardiac or skeletal muscle cells. (wikipedia.org)
  • Those studies showed that drug exposure causes multipotent P19 cells to differentiate into different layers of cells. (wikipedia.org)
  • Other than into neurons and astrocytes, P19 cells can also differentiate to oligodendrocytes, which can be detected using the specific markers, myelin-associated glycoprotein and 2',3'-Cyclic-nucleotide 3'-phosphodiesterase. (wikipedia.org)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • It is therefore of interest to find effective ways to produce cells which may function, proliferate, and differentiate as appropriate, while being safe from attack by a recipient's immune system. (justia.com)
  • Mouse ES cells have been widely utilized as an in vitro model to study cardiogenesis, as cardiomyocytes were found to spontaneously differentiate from ES cells after withdrawal of LIF (leukemia inhibitory factor), which functions to maintain the pluripotency of undifferentiated mouse ES cells [ 2 - 4 ]. (biomedcentral.com)
  • This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. (bdbiosciences.com)
  • In other cases, where certain cells are lacking, such as islets of Langerhans in the case of diabetes, or cells which secrete dopamine in the case of Parkinson's disease, or bone marrow cells in various hematopoietic diseases, or muscle cells in muscle wasting disease, or retinal epithelial cells in visual disorders, it would be desirable to be able to provide cells which could fulfill the desired function. (justia.com)
  • A wide variety of hematopoietic cells exist, with the major protective lineages being lymphoid and myeloid. (justia.com)
  • He has also much experience in context of homing, mobilization, and engraftment of hematopoietic stem cells and cancer research. (livedna.net)
  • Novel evidence that the mannan-binding lectin pathway of complement activation plays a pivotal role in triggering mobilization of hematopoietic stem/progenitor cells by activation of both the complement and coagulation cascades. (livedna.net)
  • Inducible nitric oxide synthase (iNOS) is a novel negative regulator of hematopoietic stem/progenitor cell trafficking. (livedna.net)
  • Moreover, signaling pathways related to neurogenesis and myogenesis were also investigated by studying gene expression or generating mutants of P19 cells. (wikipedia.org)
  • Our results suggest that Foxp3 exerts protumoral intrinsic effects in breast cancer cells and that gene-therapy-mediated blockade of Foxp3 could constitute a therapeutic strategy to improve the response of these tumors to standard treatment. (bvsalud.org)
  • In addition, heterozygous hosts having one wild-type gene and one mutated gene could be mated to obtain homozygous hosts, so that all of the cells would have the appropriate modification. (justia.com)
  • The neomycin-resistant gene driven by the cardiac α-myosin heavy chain promoter was stably transfected into ES cells. (biomedcentral.com)
  • This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
  • The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
  • In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)
  • Molecular mechanism controlling the expression of the HOX Complex gene family in human teratocarcinoma cells (N-tera2/D1). (cnr.it)
  • Morphogen gradients induce sharply defined domains of gene expression in a concentration-dependent manner, yet how cells interpret these signals in the face of spatial and temporal noise remains unclear. (elifesciences.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • After six to nine days of treatment, the relative neuronal population declines, likely because of faster proliferation of non-neuronal cells. (wikipedia.org)
  • A vast amount of research exists on the possible molecular mechanisms through which vitamin D affects cancer cell proliferation, cancer progression, angiogenesis, and inflammation. (mdpi.com)
  • The fluorescence histogram showing podoplanin expression (or Ig Isotype control staining), was derived from gated events with the forward and side light-scatter characteristics of viable cells. (bdbiosciences.com)
  • Knowing about a cell line's general characteristics and origin is essential before you start working with it. (cytion.com)
  • The P19 cell line is widely cultured in research laboratories due to its unique characteristics. (cytion.com)
  • The aim of this study was to analyze the roles of Cripto-1 in the progression and clinical characteristics in NPC clinical samples and cell lines. (biomedcentral.com)
  • We studied the role of the expression of the transcription factor FOXP3 on CD8+ T cell function and anti-tumor immunity. (unav.edu)
  • Here we show that suboptimal T cell receptor stimulation of CD8+ T cells upregulates FOXP3 in vitro. (unav.edu)
  • Cisplatin upregulated Foxp3 expression in HER2+ and triple-negative breast cancer (TNBC) cells. (bvsalud.org)
  • It was not until 1998 that American biologist James Thomson isolated and cultured human embryonic stem cells from human blastocysts for the first time [11] . (danabiosci.com)
  • This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • This led to many new kinds of experiments in many laboratories, aimed at defining the role of a normal microenvironment on cancer cells. (pas.va)
  • Her new experiments also showed that stem-like cancer cells could be grown in culture and used as 'messengers' to convey specific-DNA into the organism. (pas.va)
  • A broad range of 16 MMPs could be found in the PA1 cells and thus this cell line could be used as a positive control for general MMP experiments. (biomedcentral.com)
  • Cells were cultured and total RNA was isolated from all 6 cell lines in three independent experiments. (biomedcentral.com)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • We also developed an adenoviral vector encoding P60 (Ad.P60) that efficiently transduced breast tumor cells, reduced cell viability and migration, and improved the cytotoxic response to cisplatin. (bvsalud.org)
  • Conditioned medium from transduced breast tumor cells contained lower levels of IL-10 and improved the activation of splenic lymphocytes. (bvsalud.org)
  • Tumor cells are surrounded by the extracellular matrix (ECM) comprising of proteoglycanes and non-proteoglycanic matrix components (collagen, laminin, fibronectin and elastin). (biomedcentral.com)
  • We conducted a systematic review of the literature on the effects of vitamin D on ovarian cancer cell. (mdpi.com)
  • The two investigated ovarian cancer cell lines showed a distinctive difference in the number of expressed MMPs (2 vs. 10). (biomedcentral.com)
  • Stem cells and regenerative medicine - future perspectives. (springer.com)
  • Stem cells in regenerative medicine: introduction. (springer.com)
  • Moreover, the multipotency of P19 cells was then confirmed by injecting the cells into blastocysts of another mouse strain. (wikipedia.org)
  • Stem cells are most frequently cultured in Matrigel and in the presence of suitable exogenous factors including chemical small molecule inhibitors/activators, cytokines, and medium additives, and can be coaxed into forming organoids of corresponding organs. (danabiosci.com)
  • Organoids are in vitro miniaturized three-dimensional (3D) model systems of organs that gained huge interest in the last decade. (facellitate.com)
  • The field of the subject invention is the use of major histocompatibility complex antigen lacking cells and organs which may serve as universal donors in cellular and organ therapies including transplantation and to produce chimeric non-human mammals. (justia.com)
  • Animal cells need to be able to communicate with each other so that they can work together in tissues and organs. (elifesciences.org)
  • Cancer cells in humans may result in the patient's death if the aggressive cancer cell grows and metastasizes. (wikipedia.org)
  • In this study we investigated the expression pattern of the 23 MMPs, currently known in humans, in different gynecological cancer cell lines. (biomedcentral.com)
  • Since stem cells derived from humans are the source material of organoids, they bridge the gap between animal models and humans. (facellitate.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • Human Cripto-1, also known as teratocarcinoma-derived growth factor-1 (TDGF-1), is a member of the Epidermal growth factor-cripto FRL1 cryptic (EGF-CFC) family (Cripto in humans, FRL1 in Xenopus, and Cryptic in mice), which is indispensable for early embryonic development[ 4 ]. (biomedcentral.com)
  • 2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
  • Stem cell technology in humans derives from earlier and complementary work in animal studies. (edu.au)
  • Due to the pluripotency of P19 cells, those new derived cell lines can be ectoderm, mesoderm and endoderm-like cells. (wikipedia.org)
  • The stem cell field witnessed a genuine breakthrough when a combination of solely four transcription factors ( Oct3 / 4 , Sox2 , Klf4 and c-Myc, OSKM ) proved enough to revert, in vitro, the differentiated status of a variety of cell types back to pluripotency, giving rise to so-called induced pluripotent stem (iPS) cells. (springer.com)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • Mesenchymal stem cells with IGF-1 and TGF-β1 in laminin gel for osteochondral defects in rabbits. (livedna.net)
  • When the host lacks the ability to raise a defense against a particular disease, there may also be an interest in administering specific T-cells or B-cells or precursors thereof which may supplement the host's compromised immune system. (justia.com)
  • In addition, we summarize the recent direct reprogramming of cardiomyocytes from fibroblast cells, which provides another method for potential heart disease therapy. (biomedcentral.com)
  • The unique properties of human stem cells have aroused considerable optimism about their potential as new pathways for alleviating human suffering caused by disease and injury. (edu.au)
  • She produced chimeric mice (which she at first termed 'allophenic') by inclusion of two genetically different cells in the early mouse embryo, thereby revealing the clonal organization. (pas.va)
  • found that mouse teratocarcinoma stem cells developed normally in a normal embryo environment. (pas.va)
  • Human-induced pluripotent stem cells (iPSCs) were successfully produced by reprogramming mouse and human fibroblasts in 2006, which has had a major impact on stem cell and organoid research [12] . (danabiosci.com)
  • No. 554680, dashed line histogram) or PE Mouse Anti-Human Podoplanin (Cat. (bdbiosciences.com)
  • Immunofluorescent staining of mouse ES cell line. (bdbiosciences.com)
  • ES-E14TG2a cells (ATCC CRL-1821) were cultured, fixed, and stained with Alexa Fluor® 488 Mouse anti-SSEA-1 (pseudo-colored green) according to the Recommended Assay Procedure. (bdbiosciences.com)
  • In this article, we will discuss the fundamentals of mouse-derived P19 cells. (cytion.com)
  • In order, therefore, to obtain a purified cardiomyocyte population from mouse ES cells, several approaches have been developed. (biomedcentral.com)
  • Mouse ES cell-derived EBs were dissociated using collagenase, followed with a modified procedure by Isenberg and Klockner in 1982 to prepare the calcium-tolerant ventricular myocytes [ 18 ]. (biomedcentral.com)
  • We also profiled lncRNA and mRNA expression in three mouse male germ cell-related cell lines (F9, GC-1 and GC-2). (biomedcentral.com)
  • Cell culture and drug treatment method conditions Human neuron committed teratocarcinoma, mouse motor neuron derived cells and SMA type I pa tient fibroblasts were maintained in regular problems in Dulbeccos modified Eagle medium supplemented with 10% fetal calf serum, 1% antibiotics and two mM glutamate. (srcpathway.com)
  • Rabbit polyclonal antibodies (pAb) were used for preparing a gold-pAb conjugate, and the rGroEL-specific mouse monoclonal antibody was used as the antigen detection reagent at the ICT test line. (jsce-ip.com)
  • Both ES cells and iPS cells are pluripotent stem cells with capabilities of indefinite self-renewal and can be differentiated into almost all cell types of the body, which make them valuable for studying early developmental biology, for modeling and as therapy for human diseases. (biomedcentral.com)
  • However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)
  • We also highlight recent studies identifying the cis -regulatory elements such as enhancers at the global level and explain how their developmental activity is regulated by the cooperation of cell-type specific and ubiquitous transcription factors with extrinsic signals. (amegroups.org)
  • The developmental capacity of nuclei taken from differentiating endoderm cells of Xenopus laevis. (springer.com)
  • The developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (springer.com)
  • The developmental capacity of nuclei transplanted from keratinized skin cells of adult frogs. (springer.com)
  • revealed that lung organoids can be produced from the 3D coculturing of endothelial cells and adult bronchioalveolar stem cells [17] . (danabiosci.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • The successful cultivation of the first small intestinal organoid from Lgr5 + intestinal stem cells by Hans Clevers in 2009 ushered in the era of organoid research. (danabiosci.com)
  • Thereafter, organoids were constructed and employed as a novel research model that could be used instead of traditional cell lines and heterogeneous animal models. (danabiosci.com)
  • The differentiated ES cell cultures are heterogeneous and contain undifferentiated ES cells, which could result in teratoma formation after transplantation into the host. (biomedcentral.com)
  • Additionally, mice were vaccinated twice with dendritic cells expressing alpha-fetoprotein. (bvsalud.org)
  • RESULTS: In a preventive setting prior to tumor engraftment, vaccination with alpha-fetoprotein-expressing dendritic cells significantly decreased tumor growth in a subcutaneous model (p = .0256), but no further effects were achieved by addition of P60. (bvsalud.org)
  • CONCLUSION: In this study, we showed that vaccination with alpha-fetoprotein-expressing dendritic cells in combination with a specific inhibition of regulatory T-cells by using P60 leads to synergistic tumor inhibition and prolonged survival. (bvsalud.org)
  • Expressed in mature dendritic cells. (affbiotech.com)
  • In this study, we investigated the expression of serotoninergic receptors (5-HTR), the signaling pathway, and biological activity of 5-HT on human dendritic cells (DC), showing that immature and mature DC expressed mRNA for different serotoninergic receptors. (aai.org)
  • Cell cultures containing undifferentiated stem cells were isolated from the primary tumor which have a euploid karyotype. (wikipedia.org)
  • Researchers use P19 cells to elucidate cell signaling pathways and cellular and molecular mechanisms involved in these processes. (cytion.com)
  • It is expected that a highly organized intrinsic genetic network is responsible for controlling spermatogenesis in the testis, and that the elucidation of the underlying molecular mechanism will help us further understand male germ cell development. (biomedcentral.com)
  • Frozen P19 cell vials should be stored at below -150°C temperature in a freezer or the vapour phase of liquid nitrogen to maintain the viability of cells for the longer term. (cytion.com)
  • CM-1 or CM-ACF media can be used to freeze P19 cells using a slow freezing method that protects cells from any shock and preserve their viability. (cytion.com)
  • In any case, the respective cell types may then be selectively cultured to enrich their percentage population to eventually obtain a single cell type and culture. (justia.com)
  • In mammals, the immune systems serves as the primary defense with many different types of cells and mechanisms to protect the host. (justia.com)
  • Indeed, some cell types never express A-type lamins. (rupress.org)
  • Differential dosage-dependent roles of OTX2 in specifying regional identities and neuronal cell types during brain development. (cnr.it)
  • Pluripotent cells can be differentiated into many different cell types in vitro. (biomedcentral.com)
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • After the inhibition of the endogenous Cripto-1, the growth, cell cycle and invasion of cells were detected by MTT, FACS and Boyden chamber assay respectively. (biomedcentral.com)
  • DMEM/Ham's F12 media containing 5% Fetal bovine serum, 3.1 g/L Glucose, 1.6 mM L-Glutamine, 1.0 mM Sodium pyruvate, 15 mM HEPES and 1.2 g/L NaHCO3 is used to culture P19 cells. (cytion.com)