Loading...
  • Systems
  • The goals of this solicitation are to stimulate the use of in vitro papillomavirus replication systems for research on (1) the events of papillomavirus replication and pathogenesis and (2) the antiviral potential of experimental therapeutic agents. (nih.gov)
  • This Request for Applications (RFA), Papillomavirus In Vitro Cell Culture Systems, is related to the priority area of sexually transmitted diseases. (nih.gov)
  • Frequently these materials are just added to the cell culture medium which is according to VITROCELL® SYSTEMS a method giving relatively low sensitivity due to interaction of the particles with the medium. (nanowerk.com)
  • VITROCELL® SYSTEMS supplies in addition the required aerosol generators as well as turnkey installations. (nanowerk.com)
  • The customers of VITROCELL® SYSTEMS are leading medical and environmental research institutes as well as the pharmaceutical and other industries. (nanowerk.com)
  • Cell cycle analyses were performed using flow-cytometry and multicycle analysis software (Phoenics Flow Systems, San Diego, CA, USA). (niom.no)
  • 4 Vitrocell Systems GmbH , Waldkirch, Germany. (vitrocell.com)
  • MucilAir cells are complex 3D cell systems with intact mucocilairy system and are cultured at air-liquid interface, in contrast to the simple cell lines A549 and BEAS 2B, which are essentially immersion models. (vitrocell.com)
  • Liver researchers have already experimented with several types of bioreactor, according to Cerra, but with most systems the liver cells died. (newscientist.com)
  • HeLa
  • We have demonstrated NFP electroporation (NFP-E) of single HeLa cells within a population by transfecting them with fluorescently labeled dextran, green fluorescent protein (GFP) plasmids, proteins (BSA), and molecular beacons (DNA hairpins with fluorophores used to detect mRNA). (nanohub.org)
  • Experiments on HeLa cells confirmed that NFP-E offers single cell selectivity, high transfection efficiency (>95%), qualitative dosage control, and very high viability (92%) of transfected cells.In this presentation, the advantages of NFP-based electroporation over other transfection and bulk electroporation techniques will be discussed. (nanohub.org)
  • epithelial
  • Treatment of dental epithelial cell line, mDEC6 with bone morphogenetic protein 4 (BMP4), a known tumor inhibitor, leads to translocation of FRG1 from the nucleus to the cytoplasm [ 14 ]. (bioscirep.org)
  • Cardiac
  • Important cardiac fibroblast-cardiomyocyte interactions have been demonstrated in appositional cocultures involving direct membrane contact and/or cell fusion as well as through the use of noncontact cell culture paradigms to assess the role of soluble factors ( 12 , 13 , 17 , 42 ). (physiology.org)
  • assess
  • The objective of this study was to assess the cellular effects of cerium oxide (CeO2) particles exposed via an air-liquid interface in three relevant cell models in parallel. (vitrocell.com)
  • Studies
  • In vitro studies have established that signals induced by myocardial injury affect fibroblasts and cardiomyocytes and may regulate the degree of fibrosis and hypertrophy associated with the recovery process ( 16 , 17 , 20 , 32 ). (physiology.org)
  • It is hoped that this paper has summarized the background of xylem cell differentiation studies and has set the scene for the papers to follow in this issue. (springer.com)
  • membrane
  • The membrane inserts are placed in the VITROCELL® modules which are tempered at 37 C. The medium supply is performed either static or by permanent exchange so that the cells receive the cell culture medium from below through the membrane of the insert. (nanowerk.com)
  • This creates temporary pores on the cell membrane to facilitate dosage-controlled entry of biomolecules into the cytosol. (nanohub.org)
  • Moreover, theoretical analysis of the NFP-E delivery mechanisms revealed that application of an electric potential of a few hundred millivolts, between the NFP cantilevered tip and the region of the cell membrane in contact with the tip, creates nanopores in the cell membrane through which molecules diffuse to the cytoplasm. (nanohub.org)
  • Cancer cell invasion is measured using a commercially available BD BioCoat TM FluoroBlok TM Invasion System, where the cancer cells are grown on a basement membrane-like Matrigel TM coated filter chamber. (pharmatest.com)
  • stem cells
  • Most complex are stem cells. (coursera.org)
  • induced pluripotent stem cells, IPS cells. (coursera.org)
  • There's also ethical issues, especially if we're talking about embryonic stem cells. (coursera.org)
  • Virtual tissues services for biomedical research features high fidelity tissue simulation and modelling to grow virtual tissue from a single cell, giving unprecedented insight into lineage commitment phenomena of stem cells with insight i. (selectscience.net)
  • Furthermore
  • Furthermore, the proteins from 84-HS-1-11 and 89-T-26-17 were able to discriminate between leukaemia and normal T cells, specifically killing the former cells. (wiley.com)
  • Furthermore, dosage of the injected biomolecules into the cells is regulated through electric pulse duration. (nanohub.org)
  • human
  • Parasporal inclusion proteins from a total of 1744 Bacillus thuringiensis strains, consisting of 1700 Japanese isolates and 44 reference type strains of existing H serovars, were screened for cytocidal activity against human leukaemia T cells and haemolytic activity against sheep erythrocytes. (wiley.com)
  • Purified parasporal inclusions of the three selected strains, designated 84-HS-1-11, 89-T-26-17 and 90-F-45-14, exhibited no haemolytic activity and no insecticidal activity against dipteran and lepidopteran insects, but were highly cytocidal against leukaemia T cells and other human cancer cells, showing different toxicity spectra and varied activity levels. (wiley.com)
  • Oxygen gradient in a culture flask with human carcinoma cells. (edaphic.com.au)
  • Q-RT PCR was done for human embyonic kidney (HEK293T) cells with altered FRG1 levels to identify associated molecules. (bioscirep.org)
  • analysis
  • Analysis of the NIR luminescence properties of these MCs was performed under different experimental conditions, including in a suspension of stained cells. (archives-ouvertes.fr)
  • Professor Espinosa is also the founder and chief scientific officer of iNfinitesimal LLC, a nanotechnology company developing robust next-generation nanoscale devices, scalable nanomanufacturing tools, and microdevices for single cell transfection and analysis. (nanohub.org)
  • migration
  • These findings suggest that reduction in FRG1 expression in gastric, colon and oral cavity tumor might have a role in tumor progression, by regulating cell migration and invasiveness. (bioscirep.org)
  • primary
  • In the middle, we have primary cells. (coursera.org)
  • Primary cells that are a bit more complex are harvested from tissue. (coursera.org)
  • A robust method for single cell access to deliver genes and small molecules to primary and sensitive cells is needed to advance the state-of-the-art in personalized medicine and therapeutics. (nanohub.org)
  • BEAS-2B, A549, and MucilAir cells were exposed for 1 hour to CeO2 particles comprising a range of doses (0.04-3.0 lg/cm2) and sizes (0.75 lm and 13.8 nm primary particle size). (vitrocell.com)
  • compounds
  • Finally, the NIR-emitting Ln3+[Zn(II)MCpyzHA] compounds combine, within a single molecule, features such as cell fixation and staining abilities, good photostability and minimal sensitivity of the emission bands to the local microenvironment, and they are highly promising for establishing the next generation of imaging agents with a single biodistribution. (archives-ouvertes.fr)
  • activity
  • To further understand the nature of biologic activity seen in patients in this trial, we cultured RPMI-8226 Multiple Myeloma, and HT-29 colon cancer cells for growth in the presence of AVN944, as a means to identify cellular and molecular characteristics of response and resistance to the drug. (aacrjournals.org)
  • Dodds, J. H. (1979) Is cell cycle activity necessary for xylem cell differentiation? (springer.com)
  • invasion
  • The capacity of the cells to migrate through the Matrigel TM layer is determined and cell invasion is quantitated by post-labelling the cancer cells with a fluorescent dye. (pharmatest.com)
  • capacity
  • Recent discoveries that postnatal cardiomyocytes exhibit limited proliferative capacity and the identification of a cardiomyocyte progenitor cell source in the heart have led to reconsideration of mechanisms underlying the myocardial injury response ( 3 , 30 , 51 ). (physiology.org)
  • liver
  • Among the tissues taken, fin, liver and caudal peduncle showed good attachment with emergence of cells. (deepdyve.com)
  • Surgeons in the US are using live cells in an 'artificial liver' that could act as a temporary short-term support system outside the body for patients with liver failure. (newscientist.com)
  • Cerra and his colleagues in the Bioartificial Liver Group have found a way to take the liver cells, or hepatocytes, from the patient and grow them outside the body in an artificial support system. (newscientist.com)
  • They then bring the liver cells into contact with the blood from the patient so that the cells can carry out their functions. (newscientist.com)
  • biology
  • New techniques developed in the area of cell biology should prove useful tools in further study of this most interesting area of cytodifferentiation. (springer.com)
  • fluorescent
  • One of the key requirements for more efficient fluorescent labelling is the preservation of cell morphology, which usually requires a combination of several fixation techniques. (archives-ouvertes.fr)
  • study
  • Unisense developed a set-up for the detailed study of pericellular oxygen concentration and consumption rates of an adhering cell culture (Pettersen et.al. (edaphic.com.au)
  • support
  • ELIGIBILITY REQUIREMENTS Applications may be submitted by domestic and foreign for-profit or nonprofit organizations, public and private, such as universities, medical colleges, hospitals, laboratories, units of State and local governments, and eligible agencies of the Federal government having adequate resources or access to resources to support papillomavirus in vitro cell culture research. (nih.gov)
  • interface
  • As the cells are not covered with medium, they are exposed in the most efficient way at air/liquid interface. (nanowerk.com)
  • inflammatory
  • Under inflammatory and hyperglycemic conditions, RANKL contributed to the promotion of pro-calcific signalling, whilst TRAIL exhibited a protective influence on vascular cells. (dcu.ie)
  • Low doses of 10-7 mg Escherichia coli endotoxin (011B4) applied as intramammary infusion into single bovine quarters induced a rise in milk cell count without other inflammatory signs. (eurekamag.com)
  • lines
  • Parental cells were significantly growth inhibited at an IC50 of 0.05uM and 0.150uM for HT-29 and RPMI-8226, respectively, where the resistant lines displayed no growth inhibition upon 72 hours treatment with 1.0uM AVN944. (aacrjournals.org)
  • In the left corner, you see cell lines. (coursera.org)
  • Cell lines. (coursera.org)
  • different
  • How different doses of AVN944 in patients affect these biomarkers will be compared to effects the drug has on resistant cells versus the parental line. (aacrjournals.org)
  • influence
  • VITROCELL® is part of group "health, occupational safety and environment" to investigate influence towards health of carbon concrete. (vitrocell.com)
  • Applications
  • The presentation will also discuss the NFP technology impact in applications such as single cell gene delivery, stem cell research, drug discovery, and cell cloning. (nanohub.org)
  • several
  • These non-haemolytic but leukaemia cell-toxic strains belonged to several H-serovars including dakota , neoleonensis , shandongiensis , coreanensis and other unidentified serogroups. (wiley.com)