Vitro bacterial adhesion and biofilm formation assay. Medical search. Frequent questions

Unlike antibacterials that aim to inhibit cell growth, antivirulence therapies are based on the inhibition of bacterial virulence. (nature.com)
Aiming for therapy, we assessed the potential of quaternary ammonium surfactant-liposomes to inhibit bacterial adherence. (uni-saarland.de)
That was the first step to develop a particle to simultaneously inhibit adhesion and provide encapsulation of antibiotic drugs as combination drug delivery. (uni-saarland.de)
A practical approach utilized in this study involves the preparation of an antibacterial thin film on the surfaces, which can effectively inhibit and reduce biofilm formation and bacterial adherence. (nature.com)
The ability of the plant extracts to inhibit or destroy pre-formed bacterial biofilms was also determined. (biomedcentral.com)
Results demonstrate that very high concentrations of the most used antibiotics in treating diabetic foot infections (DFI) are required to inhibit S. aureus biofilms in vitro, which may explain why monotherapy with these agents frequently fails to eradicate biofilm infections. (biomedcentral.com)
The only antibiotics able to inhibit biofilm eradication on 50 % of isolates were ceftaroline and gentamicin. (biomedcentral.com)
Compounds originating from naturally competitive environments, such as marine biofilms, are more likely to possess greater potential to inhibit the virulence of other organisms. (solamonrenewableenergy.com)
The first method is to inhibit the initial adhesion of oral bacteria. (hindawi.com)
On the one hand, the ability of green tea polyphenols to inhibit Streptococcus mutans growth, biofilm formation, and acid production may contribute to its beneficial effects for dental caries [ 9 - 11 ]. (biomedcentral.com)

The top five pathogens associated with ETT-related pneumonia, Methicillin-Resistant Staphylococcus aureus (MRSA) , Pseudomonas aeruginosa , Klebsiella pneumonia, Acinetobacter baumannii, and Escherichia coli, were evaluated for attachment to micro-patterned and un-patterned silicone surfaces in a short-term colonization assay. (springeropen.com)
This engineered micro-pattern reduces the colonization and biofilm formation of key VAP-associated pathogens in vitro . (springeropen.com)
An ideal transmucosal implant surface exposed to the oral cavity is recommended to be highly polished to resist bacterial colonization and it is expected to allow the formation of an epithelial seal that prevents plaque accumulation leading to peri-implantitis [ 5 , 6 ]. (hindawi.com)
Regarding the effects of green tea polyphenols on the S. moorei colonization properties, it was found that biofilm formation on EGCG-treated surfaces was significantly affected, and that green tea extract and EGCG can cause the eradication of pre-formed S. moorei biofilms. (biomedcentral.com)
In this work we (1) determine the shelf-life of ready-to-use microfluidics, (2) demonstrate biofilm-like colonization on fungi, (3) describe bacterial motility on fungal hyphae (fungal highway), (4) report material-dependent bacterial-fungal colonization, (5) demonstrate germination of vacuum-sealed Arabidopsis seeds in microfluidics stored for up to 2 weeks, and (6) observe bidirectional cytoplasmic streaming in fungi. (springer.com)

In this study, our goal was to assess the in vitro antimicrobial, anti-adhesive and anti-biofilm abilities of BS produced by Lactobacillus jensenii and Lactobacillus rhamnosus against clinical Multidrug Resistant (MDR) strains of Acinetobacter baumannii , Escherichia coli , and Staphylococcus aureus (MRSA). (biomedcentral.com)
In one study, 15 Lactobacillus strains were tested in vitro for BS production. (biomedcentral.com)
Antimicrobial drug resistance is a global challenge for the 21st century with the emergence of resistant bacterial strains worldwide. (cdc.gov)
To evaluate antibacterial behavior, we used two bacterial strains Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus aureus ( S. aureus ). (nature.com)
The Ti-Cu films displayed superior antibacterial ability, with a 99.9% reduction (equivalent to a 5-log reduction) in bacterial viability after 2 h compared to Ti alone against both bacterial strains. (nature.com)
However, excessive and indiscriminate use of antibiotics has resulted in the development of antibiotic resistance among bacterial strains 14 . (nature.com)
Interestingly, most isolates including strong biofilm producers displayed production levels of PIA (polysaccharide intercellular adhesin), the main S. epidermidis extracellular polysaccharide, similar to reference S. epidermidis strains classified as non-biofilm formers, and much lower than those classified as intermediate or high biofilm formers, possibly suggesting that high levels of PIA production do not confer a particular advantage for clinical isolates. (rossimicrolab.com)
The strains were allowed to form biofilm in vitro. (jmb.or.kr)
In this study, we analysed the antimicrobial susceptibility patterns of biofilm-producing S. aureus strains isolated from diabetic foot infections. (biomedcentral.com)
Clinical isolates of P. gingivalis strains with high dipeptidyl peptidase 4 (DPP4) expression also had a high capacity for biofilm formation and were more infective. (inra.fr)
Decreased attachment of encapsulated strains is in agreement with data of others which carefully documented enhanced adhesion to surfaces and biofilm formation in rough strains [19, 22, 23, 25, 45]. (dub-signal.com)

This study focused on the changes in biofilm activity and related metabolic pathways of S. aureus treated with lactic acid bacteria planktonic CFS (LAB-pk-CFS) and biofilm state (LAB-bf-CFS). (frontiersin.org)
However, there have not been many investigations on how pathogenic bacteria are affected by the metabolic properties of planktonic and biofilm probiotics. (frontiersin.org)
Here, we used untargeted metabolomics to investigate the potential impacts of planktonic bacteria (LAB-pk-CFS) and biofilm colonies (LAB-bf-CFS) on the metabolism of S. aureus biofilms. (frontiersin.org)
Due to the resistance of some bacteria to antibiotics, research in the field of dealing with bacterial infections is necessary. (nature.com)
2019: Researchers report the production of a new synthetic (possibly artificial) form of viable life, a variant of the bacteria Escherichia coli, by reducing the natural number of 64 codons in the bacterial genome to 59 codons instead, in order to encode 20 amino acids. (wikipedia.org)
The antimicrobial activity of the crude acetone leaf extracts was determined against six common nosocomial pathogens, namely: Gram-positive bacteria ( Bacillus cereus , Enterococcus faecalis , Staphylococcus aureus ), Gram-negative bacteria ( Escherichia coli , Pseudomonas aeruginosa , Salmonella Typhimurium) using a two-fold serial microdilution assay with p-iodonitrotetrazolium violet as growth indicator. (biomedcentral.com)
Secondary colonising bacteria then adhere to the primary colonisers resulting in biofilm formation. (springer.com)
We have determined that the ability of C. jejuni to bind to epithelial cell lines in vitro is significantly affected by the growth temperature and growth stage of the bacteria, but not by growth-medium composition. (microbiologyresearch.org)
Once S. aureus adheres to host tissues, it can form biofilms, which enable its persistence by allowing bacteria to evade host defences, impeding access to certain types of immune cells, such as macrophages, which display incomplete penetration into the biofilm matrix and "frustrated phagocytosis" [ 7 ]. (biomedcentral.com)
The study intends to explore the therapeutic potential of naturally produced compounds by competitive marine bacteria residing in marine biofilms against C. albicans biofilm. (solamonrenewableenergy.com)
Several independent studies have shown that surface-associated bacteria produce bioactive compounds with clinical importance, including antibiotics and anti-biofilm agents8,9,10. (solamonrenewableenergy.com)
It is important to develop functional transmucosal implant surfaces that reduce the number of initially adhering bacteria and they need to be modified to improve the anti-bacterial performance. (hindawi.com)
If the native host bacteria win the race, tissue cells will not be able to displace these primary colonizers, and biofilm formation will occur developing into peri-implantitis [ 4 , 5 ]. (hindawi.com)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)

These findings highlight that adding Cu to Ti can improve the antibacterial ability of the surface while inhibiting bacterial adherence. (nature.com)
Absorbance assay, crystal violet staining and adherence assay were used to detect the antifungal activity of flavopiridol. (researchsquare.com)
Several structural and secreted virulence factors play a role in S. aureus infections, which are multifactorial and depend on bacterial adherence and biofilm formation. (biomedcentral.com)
Furthermore, the lower amount of biomass and average thickness of the biofilms for the type 3 fimbriae mutants compared to the wild type and type 1 fimbriae mutant indicates that type 3 fimbriae also mediates cell-cell adherence in the biofilm. (peptide-solubility.com)

The findings demonstrated that the LAB-CFS treatment considerably slowed Staphylococcus aureus ( S. aureus ) growth and prevented it from forming biofilms. (frontiersin.org)
The effects of these NPs on Acinetobacter baumannii , Staphylococcus aureus , and Escherichia coli biofilms were studied using quantitative biofilm inhibition and disruption assays, as well as using fluorescence, -scanning electron microscopy, and atomic force microscopy. (dovepress.com)
Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. (jmb.or.kr)

The antimicrobial, anti-adhesive and anti-biofilm activities of both BS against the above mentioned MDR pathogens were determined. (biomedcentral.com)
Anti-adhesive and anti-biofilm activities were also observed for the aforementioned pathogens between 25 and 50 mg/ml. (biomedcentral.com)
Two key pathogens, MRSA and Pseudomonas aeruginosa, were evaluated for biofilm formation in a nutrient rich broth for four days and minimal media for 24 hours, respectively, on each surface type. (springeropen.com)
N-acetylcysteine inhibits growth, adhesion and biofilm formation of Gram-positive skin pathogens // Microbial Pathogenesis. (iegm.ru)
Plant extracts may have good activity per se or may be sources of effective antimicrobial compounds which can act against planktonic and/or biofilms of pathogens. (biomedcentral.com)

however, among the biofilm-producing isolates, those from catheters were the most efficient in biofilm formation. (rossimicrolab.com)
To this end, 3-hydroxy coumarin (3HC), a compound identified from the cell-free culture supernatant of the marine bacterium Brevundimonas abyssalis, was found to exhibit anti-biofilm and anti-hyphal activity against both reference and clinical isolates of C. albicans. (solamonrenewableenergy.com)
In this investigation, we obtained potent biofilm-forming bacterial isolates from the early-stage biofilm formed on three different artificial surfaces (stainless steel, high-density polyethylene, and titanium) immersed in the intake area of a coastal located power plant22. (solamonrenewableenergy.com)

0.01) reduction was demonstrated on micro-patterned surfaces for P. aeruginosa biofilms under clinically-simulated conditions when compared to un-patterned controls. (springeropen.com)
Drawing inference from such in vitro data, it has been proposed that rough implant surfaces may exhibit a greater propensity for implant-associated infections in vivo [ 5 ]. (springer.com)
The prime factor that is conducive to the pathogenesis of C. albicans is its ability to form biofilm on both biotic and abiotic surfaces, followed by other major virulence traits, such as yeast-to-hyphal transitions, filamentous morphology, wrinkle morphology, and secretion of proteolytic and lipolytic enzymes5. (solamonrenewableenergy.com)
not be significantly involved in development of uncomplicated UTIs, our results indicates that type 3 fimbriae may be a significant virulence factor in CAUTIs since they promote biofilm formation selleck chemicals llc on inert surfaces. (peptide-solubility.com)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
The bacterial attachment to the cell surfaces was evaluated microscopically. (biomedcentral.com)

The differentially expressed genes and proteins linked to S. aureus biofilms have been identified using transcriptomic and proteomic investigations ( 14 - 17 ). (frontiersin.org)

Garlic ( Allium sativum ), a popular food spice and flavoring agent, has also been used traditionally to treat various ailments especially bacterial infections for centuries in various cultures around the world. (frontiersin.org)
The capacity of S. aureus to build biofilms is correlated with the severity of infections ( 4 ). (frontiersin.org)
Several BS exhibit anti-bacterial, anti-fungal and anti-viral activities, making them appropriate candidates to combat infections [ 3 ]. (biomedcentral.com)
The presence of biofilms causes numerous problems in the field of medicine, interfering with clinical therapy of chronic and wound-related infections as well as persistent infections of various indwelling medical devices [ 15 ]. (biomedcentral.com)
Bacterial infections pose a significant threat to human health and cause various diseases. (nature.com)
Over the years, antibiotics have played a crucial role in combating bacterial infections by benefiting from technological advancements and medical knowledge 13 . (nature.com)
The results suggest that the antibiotic susceptibility patterns cannot be applied to biofilm established infections. (biomedcentral.com)
Biofilm formation is the prime cause of antibiotic resistance during C. albicans infections and treating biofilm-forming cells is challenging due to their intractable and persistent nature. (solamonrenewableenergy.com)
In conclusion, this study showed that marine biofilms are a hidden source of diverse therapeutic drugs, and 3HC could be a potent drug to treat C. albicans infections. (solamonrenewableenergy.com)
Further, virulence traits, including adhesion, hyphal, and biofilm formation enable C. albicans to access deep tissues for systemic infections. (solamonrenewableenergy.com)
Therefore, there is a critical need for alternative therapeutic agents to fight against biofilm-mediated infections and to conquer the limitations of current antifungal therapies. (solamonrenewableenergy.com)
The most frequent oral infections include gingivoperiodontal diseases including gingivitis and periodontitis, are caused by dental plaque, which is a S. mutans produced biofilm[ 9 - 11 ]. (biomedcentral.com)

We combined two highthroughput screening methods focus on anti-bacterial and anti-inflammatory to validate lots of active ingredients from different plants, and finally got a multi-herb extraction GMP which consists of the peel of Garcinia Mangostana, Magnolia Officinalis, and Punica Granatum. (cosmeticsbusiness.com)
Anti-bacterial agents. (bvsalud.org)

Additionally, it inhibits the physiological traits of the S. aureus biofilm, including hydrophobicity, motility, eDNA, and PIA associated to the biofilm. (frontiersin.org)
In addition, pyrD inactivation reduced flagellar motility and strongly affected biofilm formation by downregulating transcription of both type 1 fimbriae and curli subunit genes. (rossimicrolab.com)
Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)

Antibacterial activity of the prepared sample was assessed by determining the number of colony-forming units per milliliter (CFU/ml) using a standard viable cell count assay. (nature.com)
The plant species examined in this study had varying degrees of antibacterial activity against bacterial planktonic and biofilm forms with some having good activity against both forms. (biomedcentral.com)
A microplate dilution assay was used to determine the antibacterial activity of green tea extract and EGCG against S. moorei . (biomedcentral.com)

The antibiotic minimum inhibitory concentration (MIC) was determined for ten antimicrobial compounds, along with the minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC), followed by PCR identification of genetic determinants of biofilm production and antimicrobial resistance. (biomedcentral.com)
The compound demonstrated significant inhibitory effects on biofilms and impaired the yeast-to-hyphal transition, wrinkle, and filament morphology at the minimal biofilm inhibitory concentration (MBIC) of 250 µg mL−1. (solamonrenewableenergy.com)

Materials and Methods Candida albicans biofilms on acrylic resin plates were treated for 15 minutes with either nystatin, fluconazole, Polident, 220µM erythrosine + 1% (w/w) nano-TiO 2 + 15 J/cm 2 blue light photodynamic therapy (Ery PDT), or distilled water. (thieme-connect.de)
At 6 hours, Ery PDT reduced the number of viable C. albicans in biofilms by 0.28log 10 CFU/mL, which was equal to the effect of fluconazole and Polident. (thieme-connect.de)
Conclusion Photodynamic therapy with erythrosine + nano-TiO 2 + blue light at low light power density (15 J/cm 2 ) was as effective at inhibiting C. albicans biofilm on acrylic resin as fluconazole and Polident. (thieme-connect.de)
however, biofilm-associated C. albicans can be resistant to these agents. (thieme-connect.de)
Furthermore, the antimicrobial abilities evaluated in vitro with Streptococcus mutans ( S. mutans ), Fusobacterium nucleatum ( F. nucleatum ) and Candida albicans ( C. albicans ) using the live/dead, MTT and colony-forming units (CFUs) assay. (peerj.com)
Intriguingly, quantitative PCR analysis of 3HC-treated C. albicans biofilm revealed significant downregulation of virulence genes (hst7, ume6, efg1, cph1, ras1, als1) associated with adhesion and morphogenesis. (solamonrenewableenergy.com)
However, biofilm formation by C. albicans has been found to gain genetic resistance against most of the currently used antifungal drugs. (solamonrenewableenergy.com)

The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. (jmb.or.kr)
A modified crystal violet assay was used to determine the antibiofilm activity of the extracts. (biomedcentral.com)
Biofilm formation was quantified by crystal violet staining. (biomedcentral.com)

To establish a predictive human infected in vitro system for Cystic fibrosis (CF) lung infection, we set-up a P. aeruginosa-infected model at the air-liquid interface (ALI), constituted by the human cystic fibrosis bronchial epithelial cells (CFBE41o-) in the apical side of a transwell and human macrophages (THP-1) in the basolateral side. (uni-saarland.de)
Planktonic P. aeruginosa infection of CFBE41o- cells led to an earlier biofilm formation after 6 hours. (uni-saarland.de)
P. aeruginosa was further evaluated for biofilm formation on each surface type in a mucin-modified medium mimicking tracheal mucosal secretions. (springeropen.com)
Biofilm formation was also reduced, with 67% ( p = 0.12) and 52% ( p = 0.05) reductions in MRSA and P. aeruginosa biofilm formation, respectively. (springeropen.com)
Maslennikova I.L. Kuznetsova M.V. Nekrasova I.V. Shirshev S.V. Effect of bacterial components of mixed culture supernatants of planktonic and biofilm Pseudomonas aeruginosa with commensal Escherichia coli on the neutrophil response in vitro // Pathog Dis. (iegm.ru)
For P. aeruginosa , low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. (jmb.or.kr)

We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)
Hemolysis interferes with the assay with 1.5 g/L hemoglobin as cut-off value. (inra.fr)

S. aureus produces large biofilm formations that support its pathogenicity and confer protection and subsequently drug resistance ( 5 ). (frontiersin.org)

Their ability to capture DNA and to spread within and between bacterial species by conjugation facilitates the rapid dissemination of potentially beneficial genes through a bacterial population. (cdc.gov)
Microbial forms living in biofilms are morphologically and functionally distinct from those of free-floating (planktonic) forms of the same species. (jmb.or.kr)
Based on previous preliminary in vitro screening and on chemotaxonomy, nine species from the Myrtaceae family were selected. (biomedcentral.com)
Thus, type 3 fimbriae expression seems to generally promote biofilm formation in different bacterial species. (peptide-solubility.com)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
It should also be noted that biofilm work, especially in a species like pneumococci undergoing stationary phase autolysis, relies on a methodology for which most parameters are unknown (generation time, homogeneity of the population, metabolism etc.) and where the results can be severly influenced by minor technical changes [46]. (dub-signal.com)

Its non-specific mode of action is associated with rupture of the bacterial cell membrane resulting in leakage of intracellular components [ 12 ]. (springer.com)
In contrast, internalisation is markedly diminished at low temperature, appears to involve active invagination of the target cell membrane via pseudopod formation, and is maximal when cells of human origin are employed. (microbiologyresearch.org)
Their effects on bacterial cell membrane integrity were investigated by transmission electron microscopy and a fluorescence-based permeability assay. (biomedcentral.com)
Transmission electron microscopy analysis and a permeabilization assay brought evidence that the bacterial cell membrane was the target of green tea polyphenols. (biomedcentral.com)

Biofilms are conglomerations of bacterial cells protected by self-synthesized extracellular polysaccharide matrices (EPS). (biomedcentral.com)
[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)

Global transcriptomic analysis on bone marrow-derived macrophages (BMDM) in a gentamycin protection assay at six time points unveiled the presence of three sequential host response waves: an early transient regulatory gene module followed by sustained and late effector responses. (nimml.org)

Microbial biofilms are resilient, immune-evasive, often antibiotic-resistant health challenges, and increasingly the target for research into novel therapeutic strategies. (jmb.or.kr)

Real time RT-PCR and western blot assays were conducted to evaluate gtfB and gtfC genes transcription and translation before and after HDA treatment. (biomedcentral.com)

assay and correlation with clinical manifestations. (microbiologyresearch.org)
The neuraminidase upregulation found in this work is also in accordance with the observed impact of sialic acid and the nanAB regulon on pneumococcal biofilm, even if again no obvious correlation can be drawn between the two putatively involved regulatory events [10]. (dub-signal.com)

In fact, biofilms were resistant to antibiotics at concentrations 10-1000 times greater than the ones required to kill free-living or planktonic cells. (biomedcentral.com)
The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)

Flavopiridol also exhibited antifungal activity by restricting fungal growth and limiting fungal biofilm formation and conidial adhesion. (researchsquare.com)

AuNPs also showed biofilm inhibition, with the highest of 98% in S. aureus . (dovepress.com)
This is the first report on rapid and efficient synthesis of AgNPs, AuNPs and AgAuNPs from P. zeylanica and their effect on quantitative inhibition and disruption of bacterial biofilms. (dovepress.com)

AZD1152 order Understanding the mode of bacterial growth in vivo during Farnesyltransferase infection is important in relation to future therapeutic measures. (peptide-solubility.com)

In this project a combination of recently developed and new mathematical modeling frameworks, metabolic pathway reconstruction, and in vitro anaerobic experimentation is used to predict the risk of enteric diseases and to prototype rationally-designed fecal transplantation strategies for risk minimization. (umassmed.edu)
Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. (jmb.or.kr)
For B. burgdorferi , we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. (jmb.or.kr)

Upon tobramycin treatment, the bacterial killing was higher on an abiotic surface (plastic plates) than on cell surface, which suggests an overestimation of the drug efficacy in cell-free assays. (uni-saarland.de)

Both BS also displayed anti-adhesive and anti-biofilm abilities against A. baumannii , E. coli and S. aureus . (biomedcentral.com)
Field emission scanning electron microscopy (FE-SEM) images verified that both E. coli and S. aureus cells were physically deformed and damaged the bacterial cell ultrastructure was observed. (nature.com)
Altogether, our results suggest that the crucial role played by the pyrD gene in AIEC virulence, and the presence of structural differences between E. coli and human DHOD allowing for the design of specific inhibitors, make E. coli DHOD a promising target for therapeutical strategies aiming at counteracting chronic inflammation in CD by acting selectively on its bacterial triggers. (rossimicrolab.com)
Fauchere J.-L. , Kervella M. , Rosenau A. , Mohanna K. , Veron M. Adhesion to HeLa cells of Campylobacter jejuni and C coli outer membrane components. (microbiologyresearch.org)
Also in E. coli , the recently discovered ability to express type 3 fimbriae, mediated by conjugative plasmids, was found to profoundly enhance biofilm formation [16, 17]. (peptide-solubility.com)

En mayo de 2007 obtuve el Certificate of Training in Molecular Biological Techniques en el Department of Molecular Biology y desde Junio de 2008 a Junio de 2009 completé el Certificate in Clinical Research en el Center for Translational Science Activities en Mayo Clinic College of Medicine, Rochester Minnesota. (unav.edu)
Un 40% de esos trabajos han sido publicados en revistas del primer cuartil entre las que destacan New England Journal of Medicine, Lancet, Lancet Infectious Diseases, Clinical Infectious Diseases, Clinical Microbiology Reviews, Antimicrobial Agents and Chemotherapy, Journal Clinical Microbiology, Journal Antimicrobial Chemotherapy, Mayo Clinic Proceedings y Malaria Journal. (unav.edu)

AgNPs also showed good biofilm disruption, with the highest of 88% in A. baumannii . (dovepress.com)
A microbial biofilm is a community of adherent microbial cellular forms with properties that help protect the microbial community from disruption by physical, chemical, or immunological attack. (jmb.or.kr)

In natural environmental conditions, marine biofilms consist of multispecies bacterial communities in close proximity, with large metabolite and protein exchange, as well as coordinated lifestyles. (solamonrenewableenergy.com)
Dozens of bacterial factors are involved in H. pylori molecular pathogenesis (i.e. flagella, urease, catalase, neutrophil-activating protein Nap-A, vacA and cagA). (nimml.org)
Once inside the host cells, it localizes under the point of bacterial attachment and interacts with the protein zonulin (ZO-1) and the junctional adhesion molecule (JAM) [ 4 ]. (nimml.org)

The reactive organosulfur compounds form disulfide bonds with free sulfhydryl groups of enzymes and compromise the integrity of the bacterial membrane. (frontiersin.org)
However, these compounds are typically associated with seawater or sediment, where the residing bacterial community experiences less competition. (solamonrenewableenergy.com)

These complex interactions may benefit or harm the interacting bacterial groups and aid the bacterial consortia in maintaining ecological equilibrium. (solamonrenewableenergy.com)
For example, resident bacterial communities establish either cooperative (beneficial) or competitive (harmful) interactions, which affect biofilm succession, biomass, and resistance to stress. (solamonrenewableenergy.com)

Additionally, biofilm cells display increased tolerance to antibiotics [ 8 ]. (biomedcentral.com)

Previous workers have shown that serial passage in vitro leads to attenuation of virulence-associated attributes such as expression of pili, invasion of human epithelial cell lines and the stimulation of endothelial cell proliferation. (microbiologyresearch.org)
Adhesion of FITC-labeled S. moorei to oral epithelial cells was monitored by fluorometry. (biomedcentral.com)

The functional similarity between human and bacterial DPP4 was confirmed using 124 potential peptide substrates. (inra.fr)

The role of human chorionic gonadotropin in regulation of naïve and memory T cells activity in vitro // International Immunopharmacology. (iegm.ru)
2006: Researchers engineer a synthetic circuit that promotes bacterial invasion of tumour cells. (wikipedia.org)
Cytotoxicity of extracts was determined against Vero kidney cells using a colorimetric tetrazolium-based assay. (biomedcentral.com)
2012 ). In vitro , flavopiridol suppresses the interaction between leukocytes and endothelial cells in the model of murine hepatitis, which is a crucial step in the initiation of inflammation (Schmerwitz et al. (researchsquare.com)
Konkel M. E. , Joens L. A. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. (microbiologyresearch.org)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Innate immune cells play a decisive role in host fate by enabling high bacterial loads and minimizing the immunological impact of this burden in the gastric mucosa. (nimml.org)

Biofilm growth plays an important role during infection by providing defence against several antagonistic mechanisms. (biomedcentral.com)
During chronic H. pylori infection hosts develop complex immune responses to cope with bacterial persistence that result in a variety of outcomes ranging from peaceful co-existence to detrimental disease. (nimml.org)

Bacterial morphology was examined using an SEM. (hindawi.com)

Comparison of anti-pertussis toxin ELISA and agglutination assays to assess immune responses to pertussis // Infectious Diseases. (iegm.ru)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)
Understanding the physical and molecular cues that initiate the formation and function of branching structures and resolve the underlying mechanisms of growth and transport in branched tissues will benefit relevant industries including those involved in fermentation, biofuel production, and health care. (springer.com)

Anatomy24

Organisms39

Diseases13

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes61

Disciplines and Occupations1

Technology, Industry, Agriculture13

Information Science3

Health Care2

Inhibit10

Colonization5

Strains11

Bacteria16

Adherence4

Staphylococcus3

Pathogens5

Isolates3

Surfaces6

Expressed genes1

Infections12

Anti-bacterial2

Motility3

Antibacterial activity3

Inhibitory concentration2

Albicans7

Crystal-violet3

Aeruginosa6

Hemolysis2

Aureus produces1

Species6

Cell membrane4

Extracellular2

Macrophages1

Antibiotic1

Western blot1

Correlation2

Planktonic cells3

Fungal1

Inhibition2

Vivo1

Metabolic3

Abiotic1

Coli5

Clinical2

Disruption2

Protein3

Compounds2

Interactions2

Additionally1

Epithelial2

Functional1

Cells7

Infection2

Morphology1

Immune1

Growth2