• In in vitro and in vivo experiments, bleomycin has been shown to cause cell cycle arrest in G2 and in mitosis. (nih.gov)
  • Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
  • We investigated the ability of the ATR inhibitor, AZD6738, to prevent the gemcitabine-induced intra-S-phase checkpoint activation and evaluated the antitumor potential of this combination in vitro and in vivo In PDAC cell lines, AZD6738 inhibited gemcitabine-induced Chk1 activation, prevented cell-cycle arrest, and restrained RRM2 accumulation, leading to the strong induction of replication stress markers only with the combination. (nih.gov)
  • Gambogic acid, the major active ingredient of gamboge, a brownish to orange resin exuded from the Garcinia hanburyi tree in Southeast Asia, possesses significant anticancer activity both in vitro and in vivo. (dovepress.com)
  • Multiple preclinical studies in vitro and in vivo have proven curcumin to be effective against various cancers. (wellnessresources.com)
  • Emerging evidence suggests that cancer stem cells (CSCs), a subpopulation of tumor cells, have the properties of self-renewal, heterogeneous progeny, drug-resistance, and carcinogenesis in vitro and in vivo ( 4 , 5 ). (spandidos-publications.com)
  • SAMHD1 is acetylated at residue lysine 405 (K405) in vitro and in vivo by an acetylatransferase, arrest defective protein 1 (ARD1). (oncotarget.com)
  • In near future in vivo screening for its anticancer activity will be warranted and a linkage map will be developed between in vivo and in vitro studies. (ijpsonline.com)
  • By using cell lines belonging to different hematologic malignancies and solid tumors, we demonstrated ex vivo that the combination of KPT-330 and CS (K+CS) could induce unique and significant antitumor effect at much lower dose of KPT-330 (at 25% of the dose used in the clinic), thereby potentially mitigating prohibitive clinical AEs (Figure 1a-d, and e-g) . (confex.com)
  • This significant synergetic antitumor effect observed with K+CS ex vivo was also validated in vivo by using an NSG mouse model of mantle cell lymphoma (Figure 1h) . (confex.com)
  • Moreover, the K+CS combination did not show this potent toxic effect on non-malignant cells in vivo and was safe without inducing toxicity to normal organs in NSG mice. (confex.com)
  • When primed by inhibition of their antiapoptotic proteins using genetic (BCL-2 or BCL-XL deletion or BAD overexpression) or pharmacologic (navitoclax) means, apoptosis resulted upon p53 restoration in vitro and in vivo. (nih.gov)
  • Considering that these isolates showed different profiles, both NAV and TDL could be useful for in vitro and in vivo assays that might shed some light on the biology of the parasite. (unav.edu)
  • In conclusion, TDL and NAV are novel Leishmania isolates that might be useful for in vitro and in vivo assays that will allow a better understanding of the parasite biology in Mediterranean areas. (unav.edu)
  • The evidence for these anticancer effects, in vitro , in vivo , and clinical are summarised, and the putative mechanisms of their action outlined. (ecancer.org)
  • In this study, we evaluate the efficacy of ACY-1215, a histone deacetylase 6 inhibitor (HDAC6i), to attenuate MUM cell growth in vitro and in vivo, and elucidate the underlying molecular mechanisms. (biorxiv.org)
  • Treatment of OMM2.5 MUM cells with ACY-1215 resulted in a significant ( p = 0.0001), dose-dependent reduction in cell survival and proliferation in vitro , and in vivo regression of primary OMM2.5 xenografts in zebrafish larvae. (biorxiv.org)
  • In agreement, pharmacological inhibition of MITF signaling with ML329, significantly reduced OMM2.5 cell survival and viability in vitro ( p = 0.0001) and in vivo ( p = 0.0006). (biorxiv.org)
  • From the in vivo study, results showed that treatment of 300 mg/kg of vanillin by intra-peritoneal injection caused the rats to be unconscious without exerting any negative effect on blood cells, kidney and liver. (upm.edu.my)
  • Our data showed that NBT suppressed CCA cell proliferation in vitro and in vivo . (ijbs.com)
  • Os estudos in vitro estão livres da complexidade e fatores de confusão próprios dos modelos in vivo e ensaios clínicos. (bvsalud.org)
  • In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
  • and cell proliferation, and commit to cell cycle arrest because of AP-1 repression. (wikipedia.org)
  • Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
  • The role of the mTOR pathway in estrogen-driven proliferation and effects of combining RAD001 and letrozole were examined in vitro in two breast cancer models. (aacrjournals.org)
  • Effects of RAD001 and letrozole (alone and in combination) on the proliferation and survival of MCF7/Aro and T47D/Aro cells were evaluated using proliferation assays, flow cytometry, immunoblotting, and apoptosis analyses. (aacrjournals.org)
  • The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
  • End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
  • The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
  • Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
  • In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. (who.int)
  • However, Tamoxifen Z isomer enters into the cell, competitively binding with ER to form receptor complexes, inhibiting that estrogen plays an role, and inhibiting proliferation of breast cancer cells. (lookchem.com)
  • Curcumin, a natural polyphenolic compound derived from turmeric (Curcuma longa L), has proven to be a modulator of multiple intercellular signalling pathways linked to inflammation, to proliferation, growth, invasion, drug sensitivity, angiogenesis and metastasis of cancer cells. (researchgate.net)
  • Whey and collagen hydrolysates inhibit melanoma cell proliferation. (greenmedinfo.com)
  • In vitro impact of a whey protein isolate (WPI) and collagen hydrolysates (CHs) on B16F10 melanoma cells proliferation. (greenmedinfo.com)
  • OBJECTIVE: Investigation of the inhibitory capacity on melanoma cells (B16F10) proliferation and the influence on % distribution of cell cycle phases, in the presence of various concentrations of whey protein isolate (WPI), bovine collagen hydrolysate (BCH) or its fractions. (greenmedinfo.com)
  • K405R mutant expressing cancer cells have reduced G1/S transition and slower proliferation compared to wildtype. (oncotarget.com)
  • Collectively, these findings suggest that SAMHD1 acetylation enhances its dNTPase activity and promotes cancer cell proliferation. (oncotarget.com)
  • As activation of c-myc expression promotes cell proliferation and blocks differentiation, it may positively regulate cell growth. (wdv.com)
  • Development of oral cancer is associated with excessive cell proliferation, deregulation in cellular differentiation, insufficient apoptosis and genomic instability. (ijpsonline.com)
  • Furthermore, the expression of the CYCA gene (involved in cell cycle and proliferation) was significantly downregulated in NAV and TDL isolates. (unav.edu)
  • Apigenin Restrains Colon Cancer Cell Proliferation via Targeted Blocking of Pyruvate Kinase M2-Dependent Glycolysis. (spandidos-publications.com)
  • Inhibitory effect of EESR on cancer cell growth and proliferation was determined by water-soluble tetrazolium salt assay. (jcpjournal.org)
  • EESR possessing antioxidant activity efficiently inhibits proliferation of HT29 cells by inducing both cell cycle arrest and apoptosis. (jcpjournal.org)
  • 6 , 7 Therefore, the inhibition of cancer cell proliferation by inducing cell cycle arrest and apoptosis is being an important preventive strategy against cancer. (jcpjournal.org)
  • Methods used to study the cytotoxic effects include cytotoxicity assay, double staining cell morphological analysis, cell cycle analysis, apoptosis test and cell proliferation assay. (upm.edu.my)
  • Here, we demonstrated that chaetocin dramatically inhibited the proliferation in ESCC cells by causing cycle arrest in the M phase and activating the caspase-dependent apoptosis signaling pathway in vitro , and we also found that chaetocin induced the accumulation cellular reactive oxygen species (ROS). (aging-us.com)
  • Moreover, the MST1/2 inhibitor XMU-MP-1 not only partially rescued the inhibitory effect chaetocin-induced proliferation, but also rescued the chaetocin-induced apoptosis in ESCC cells. (aging-us.com)
  • Cholangiocarcinoma (CCA) is a type of hepatobiliary cancer characterized by uncontrolled cell proliferation, with a poor prognosis and high mortality. (ijbs.com)
  • Colony formation and Edu assay indicated that NBT inhibited cell proliferation. (ijbs.com)
  • Telmisartan inhibits cancer cell proliferation, but the underlying mechanisms in PDAC, remain unknown. (bvsalud.org)
  • Material and Methods: In the present study, we evaluated the effects of telmisartan on human PDAC cell proliferation in vitro. (bvsalud.org)
  • Results: Telmisartan inhibited the proliferation of these cells via blockade of the G0 to G1 cell cycle transition. (bvsalud.org)
  • Conclusion: In conclusion, telmisartan inhibits human PDAC cell proliferation by inducing cell cycle arrest. (bvsalud.org)
  • Angiotensin II type 1 (AT1) receptor blocker (ARB), telmisartan may inhibit cancer cell proliferation, but the mechanisms by which telmisartan affects various cancers remain unknown. (bvsalud.org)
  • In our experiments, telmisartan inhibited the proliferation of HuCCT-1 and TFK-1 cells. (bvsalud.org)
  • In conclusion, telmisartan was shown to inhibit human CCA cell proliferation by inducing cell cycle arrest. (bvsalud.org)
  • Telmisartan, a widely used antihypertensive drug, is an angiotensin II type 1 (AT1) receptor blocker (ARB) that might inhibit cancer cell proliferation, but the mechanisms through which telmisartan affects various cancers remain unknown. (bvsalud.org)
  • In our experiments, telmisartan inhibited the proliferation of HLF, HLE and HepG2 cells, which represent poorly differentiated types of HCC cells. (bvsalud.org)
  • Cytotoxicity and cell proliferation were assessed using WST-1 and Alamar blue for each cell type. (cdc.gov)
  • Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)
  • Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
  • Moreover, synergistic growth inhibition was identified in a panel of 5 mouse and 7 human PDAC cell lines using both Bliss Independence and Loewe models. (nih.gov)
  • 1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)
  • In cancer progression, FOXO3a inhibition stimulates cell transformation and angiogenesis ( 9 ). (spandidos-publications.com)
  • Therefore, we used OEO and its main component, thymol, to explore their effects on cell growth inhibition and anticancer activity. (irost.org)
  • The mechanism of action for this antifungal activity is through the decrease of ergosterol synthesis, required for membrane integrity of fungal cells, via inhibition of the lanosterol 14 alpha-demethylase (14DM) catalyst. (ecancer.org)
  • In contrast, AJs found between leader cells in the same migrating sheet have cortical flows aligned in the same direction, and no such mechanical inhibition takes place. (ibecbarcelona.eu)
  • Furthermore, we observed that inhibition of RUNX1/ETO in Kasumi1 cells and in RUNX1/ETO positive primary acute myeloid leukemia patient samples leads to up-regulation of miR144/451. (uni-frankfurt.de)
  • Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)
  • MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) tests demonstrated that the GA-mPEG 2000 micelle formulation had obvious cytotoxicity to tumor cells and human umbilical vein endothelial cells. (dovepress.com)
  • Despite having a limited effect on L929 normal cells, OEO/thymol induced cytotoxicity in MDA-MB231 breast cancer monolayers (2D) and to a lesser extent in MDA-MB231 spheroids (3D). (irost.org)
  • To investigate the mechanism of EESR-mediated cytotoxicity, HT29 cells were treated with various concentrations of EESR and the induction of cell cycle arrest and apoptosis was analyzed by flow cytometry, 4,6-diamidino-2-phenylindole staining, and Western blot analysis. (jcpjournal.org)
  • Here, we demonstrate that the dNTPase activity of SAMHD1 is regulated by acetylation, which promotes cell cycle progression in cancer cells. (oncotarget.com)
  • Microinjection of Rb in G1 phase reversibly arrests cell-cycle progression. (wdv.com)
  • Mechanistically, protein profiling through mass spectroscopy revealed that K+CS uniquely affects the cellular pathways of DNA damage repair, DNA synthesis and cell cycle progression. (confex.com)
  • 12 The activation of cyclin/CDK complex accelerates cell cycle progression, which are regulated by phosphorylation. (jcpjournal.org)
  • Further analysis with GenomeLab GeXP genetic system on brain tissues showed that the expression of most xenobiotic metabolism, cell progression, tumour suppressor, DNA damage and inflammation genes was maintained at normal level. (upm.edu.my)
  • JDP2 plays a role in cell differentiation in several systems. (wikipedia.org)
  • Ectopic expression of JDP2 inhibits the retinoic acid-induced differentiation of F9 cells and adipocyte differentiation. (wikipedia.org)
  • By contrast, JDP2 induces terminal muscle cell differentiation in C2 myoblasts and reduces the tumorigenicity of rhabdomyosarcoma cells and restored their ability to differentiate into myotubes. (wikipedia.org)
  • Methylome mapping suggests that JDP2 plays a role in cell progenitor differentiation of megakaryocytes. (wikipedia.org)
  • This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
  • HCK expression is up-regulated at the transcriptional level during myeloid differentiation of hematopoietic cells. (uni-frankfurt.de)
  • Abstract: A network of lineage-specific transcription factors and microRNAs tightly regulates differentiation of hematopoietic stem cells along the distinct lineages. (uni-frankfurt.de)
  • The inhibitors demonstrated promising effects in vitro with reduced viability, increased differentiation and cell death of neuroblastoma cells. (lu.se)
  • Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
  • Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
  • Mouse p53 protein negatively regulates the JDP2 promoter in F9 cells as part of the JDP2˗p53 autoregulatory circuit. (wikipedia.org)
  • Ataxia telangiectasia and Rad3-related protein (ATR), the apical kinase of the intra-S-phase DNA damage response, plays a central role in safeguarding cells from replication stress and can therefore limit the efficacy of antimetabolite drug therapies. (nih.gov)
  • Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as from non-treated stem cells. (wjgnet.com)
  • A striking decrease in Skp2 mRNA and protein expression and reciprocal increase in p27 protein level were observed in three PA-treated prostate cancer cells. (iiarjournals.org)
  • This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
  • FoxO3a silencing resulted in decreased protein expression of FoxO3a, increased in vitro carcinogenesis and CSC characteristics, with no appreciable effects on AMPK and ACC phosphorylation, and displayed similar activities to those neutralizing the effects of casticin on in vitro carcinogenesis and CSC characteristics. (spandidos-publications.com)
  • SAM domain and HD domain containing protein 1 (SAMHD1) is a deoxynucleotide triphosphohydrolase (dNTPase) that inhibits retroviruses by depleting intracellular deoxynucleotide triphosphates (dNTPs) in non-cycling myeloid cells. (oncotarget.com)
  • Abrogation by c-myc of G1 phase arrest induced by RB protein but not by p53. (wdv.com)
  • Replacement of wild-type RB can suppress the tumorigenicity of some of these cells, suggesting that the RB protein (Rb) may negatively regulate cell growth. (wdv.com)
  • The c-myc protein is localized in the nucleus and can physically associate with RB protein in vitro, hence c-myc may functionally antagonize RB function. (wdv.com)
  • Acquisition of a point mutation in the propeller region of the K13 protein after ≈30 drug pressure cycles was on the critical path to artemisinin resistance in the F32-ART lineage ( 10 ). (cdc.gov)
  • The human hemopoietic cell kinase (HCK) is a member of the src family of protein tyrosine kinases specifically expressed in myeloid cells and to a minor extent in B-lymphoid cells. (uni-frankfurt.de)
  • Electrophoretic mobility shift analysis using nuclear extracts obtained from RAW264 cells and from the promonocytic cell line U-937 revealed the formation of at least three distinct protein-DNA complexes at each of these sites, one of which was found to contain the transcription factor Sp1. (uni-frankfurt.de)
  • Note that running and transferring of Phos-tag polyacrylamide gels is inconsistent and cell cycle-dependent changes in protein abundance observed in these panels may not accurately reflect changes in protein abundance. (figshare.com)
  • This blockade was accompanied by a strong decrease in cell cycle-related protein, especially G1 cyclin, cyclin D1, and its catalytic subumits, Cdk4 and Cdk6. (bvsalud.org)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
  • These potent effects are driven by curcumin's ability to induce G2/M cell cycle arrest, induce autophagy, activate apoptosis, disrupt molecular signaling, inhibit invasion and metastasis, and increase the efficacy of current chemotherapeutics. (wellnessresources.com)
  • There is evidence that at the clinically relevant doses, itraconazole has potent anti-angiogenic activity, and that it can inhibit the Hedgehog signalling pathway and may also induce autophagic growth arrest. (ecancer.org)
  • In conclusion, vanillin could induce cytotoxic effects on colorectal cancer cells. (upm.edu.my)
  • ESP1 , ESP1-myc13 and pGAL-SWE1 ESP1-13myc cells were grown in YEP + raffinose, arrested in mitosis with nocodazole and switched to YEP + galactose media to induce expression of Swe1. (figshare.com)
  • Notably, telmisartan did not induce apoptosis in HuCCT-1 cells. (bvsalud.org)
  • Linalool induced the loss of cell viability or cell death by a clear dose and time dependent manner. (ijpsonline.com)
  • Linalool treatment in various concentrations in vitro in keratinforming tumor cell line cells was able to reduce cell viability as compared to its counterparts. (ijpsonline.com)
  • Significant reduction in cell viability was found to occur with graphene at doses ≥ 12.5 µg/ml. (cdc.gov)
  • Rigosertib reduced cell viability of neuroblastoma cells and caused cell cycle arrest in the G2/M phase. (lu.se)
  • Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
  • The role of the mTOR pathway in estrogen response was evaluated in aromatase-expressing MCF7/Aro breast cancer cells by immunoblotting. (aacrjournals.org)
  • Treatment of MCF7/Aro cells with estradiol or androstenedione caused modulation of the mTOR pathway, a phenomenon reversed by letrozole or RAD001. (aacrjournals.org)
  • Mice lacking the gene for Dmp1 commonly develop lung tumors, and Dmp1 has been identified as a regulator of the Arf-p53 pathway in vitro. (eurekalert.org)
  • We have demonstrated that the hDMP1 gene is hemizygously deleted in a significant percentage of mouse and human non-small-cell lung carcinomas, especially those which retained the intact Arf-p53 pathway," explains Dr. Inoue. (eurekalert.org)
  • We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
  • VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
  • However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (who.int)
  • Li L , Jiang H , Li Y , Xiang X , Chu Y , Tang J , Liu K , Huo D , Zhang X , . Chaetocin exhibits anticancer effects in esophageal squamous cell carcinoma via activation of Hippo pathway. (aging-us.com)
  • We further revealed that chaetocin triggered the activation of Hippo pathway in ESCC cells, which is characterized by elevated phosphorylation levels of almost all core proteins in Hippo pathway, such as MST1 (Thr183), MST2 (Thr180), MOB1 (Thr35), LAST1 (Thr1079 and Ser909) and YAP (Ser127), ultimately leading to decreased nuclear translocation of YAP. (aging-us.com)
  • To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
  • Phosphorylated ATF2 inhibits the formation with JDP2 in vitro while phosphorylated JDP2 undergoes proteosomal degradation. (wikipedia.org)
  • JDP2 has INHAT activity and inhibits histone methylation in vitro. (wikipedia.org)
  • Lonafarnib inhibits the farnesylation of H-Ras and the attachment of Ras to the cell membrane. (uni-marburg.de)
  • However, whether casticin inhibits in vitro carcinogenesis and cancer stem cell (CSC) characteristics, and whether casticin activates FoxO3a in small cell lung cancer (SCLC) cells remain unclear. (spandidos-publications.com)
  • However, whether casticin inhibits in vitro carcinogenesis and CSC characteristics in the SCLC H446 cell line, and activates FoxO3a remains unclear. (spandidos-publications.com)
  • Co-injection of c-myc, but not EJ-ras, c-fos or c-jun, inhibits the ability of Rb to arrest the cell cycle. (wdv.com)
  • The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
  • RESULTS: The inhibitory concentrations for 50% of B16F10 cells (IC(50)) ranged from 0.19 to 156.9 mg/mL for all these proteins evaluated. (greenmedinfo.com)
  • Acetylated SAMHD1 wildtype proteins have enhanced dNTPase activity in vitro , whereas non-acetylated arginine substituted mutants (K405R) do not. (oncotarget.com)
  • This blockade was accompanied by a marked decrease in the levels of cyclin D1, cyclin E and other cell cycle-related proteins. (bvsalud.org)
  • In vitro ubiquitination assay showed a decreased p27 ubquitination in PA-treated prostate cancer cells. (iiarjournals.org)
  • 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay was conducted to find out the half maximal inhibitory concentration of linalool in keratinforming tumor cell line. (ijpsonline.com)
  • We repurposed ibudilast, a specific MIF inhibitor, and treated patient derived cell lines. (nature.com)
  • This study showed that linalool was a potent growth inhibitor of keratin-forming tumor cell line. (ijpsonline.com)
  • Broader application of KPT-330 (selinexor), a first in class CRM1 inhibitor recently approved for multiple myeloma and diffuse large B-cell lymphoma (DLBCL), has been limited by substantial adverse effects (AEs). (confex.com)
  • EESR induced cell cycle arrest at G2/M phase in a dose-dependent manner by modulating cyclin B, cyclin-dependent kinase 1 (CDK1), and CDK inhibitor p21 expression. (jcpjournal.org)
  • Previous studies showed that vanillin was a good red blood sickle cell inhibitor, anti-microbial agent and anti-mutagen. (upm.edu.my)
  • In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
  • In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA. (irost.org)
  • Mitochondrial apoptotic priming is a key determinant of cell fate upon p53 restoration. (nih.gov)
  • Using a panel of Kras-driven, p53 restorable cell lines derived from genetically engineered mouse models of lung adenocarcinoma and sarcoma (both of which undergo cell cycle arrest upon p53 restoration), as well as lymphoma (which instead undergo apoptosis), we show that the level of mitochondrial apoptotic priming is a critical determinant of p53 reactivation outcome. (nih.gov)
  • We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
  • The combined treatment of Flavopiriol and Lonafarnib in BG1-cells showed a slightly increased cell cycle arrest in the subG1-fraction in selected concentrations. (uni-marburg.de)
  • Different concentrations of vanillin showed arrest of cell cycle at different checkpoints. (upm.edu.my)
  • Pdx-Cre (KPC) mouse cancer cell line, significantly extending survival. (nih.gov)
  • By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
  • CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)
  • Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
  • In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
  • Importantly, Dmp1 overexpression inhibited growth of human lung cancer cells with K-ras mutation and wild-type Arf and p53. (eurekalert.org)
  • In the present study, the TCEE treatment induced cell cycle arrest and suppressed cell growth on both Hep3B and HepJ5 cells. (hindawi.com)
  • CONCLUSION: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. (who.int)
  • If the estrogen receptor (ER) is present in breast cancer cells, estrogen enters into tumor cells and binds with ER, promoting mRNA and DNA synthesis of tumor cells, stimulating tumor cell growth. (lookchem.com)
  • Changes in cell cycle phases were characterized by a general decrease in the G2/M phase that emphasizes growth arrest, some increase in phase S (BCH-P1 and F4) but a strong increase in G0/G1 phase for BCH-P1 and F4. (greenmedinfo.com)
  • Conversely, FOXO3a overexpression suppresses cancer cell growth, induces apoptosis, and reduces tumor size by regulating downstream effectors ( 10 ). (spandidos-publications.com)
  • Early growth 1 phase arrest in the cell cycle of keratin-forming tumor cell line cells was confirmed on dose and time dependent manner when compared with control during linalool treatment. (ijpsonline.com)
  • EESR showed significant antioxidant activity and inhibitory effect on HT29 cell growth in a dose-dependent manner. (jcpjournal.org)
  • The abnormal cell growth is one of the critical characteristics of cancer for invasion or spreading out to other parts of the body. (jcpjournal.org)
  • Our findings provide evidence that ACY-1215 and ML329 are efficacious against growth and survival of MUM cells and are potential therapeutic options for MUM. (biorxiv.org)
  • In our study, we identified that the drug ACY-1215 prevents the growth of UM cells from the liver. (biorxiv.org)
  • In studies using animals or cells grown in labs, cinnamon has shown promise for its ability to slow cancer growth and even kill tumor cells. (webmd.com)
  • Telmisartan was also shown by receptor tyrosine kinase and angiogenesis arrays to reduce the phosphorylation of epidermal growth factor receptor (EGFR), and miRNA expression was markedly altered by telmisartan in PK-1 cells. (bvsalud.org)
  • Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)
  • Results and Conclusions: The adaptation process was characterized by initial cell population growth. (lu.se)
  • The malformations have a normal endothelial cell growth cycle that affects the veins, the capillaries, or the lymphatics, and they do not involute. (medscape.com)
  • The researchers demonstrated that Dmp1 also showed haploid insufficiency, meaning that cancer developed even when one copy of the gene was present, in samples from lung cancer patients with non-small-cell lung cancers. (eurekalert.org)
  • Since hDMP1 loss-of-heterozygosity lung cancer cells retain one allele of the hDMP1 locus, this gene might be a promising target for future drug development. (eurekalert.org)
  • This gene thus plays a role in both cell-cycle and epigenetic regulation. (cancerindex.org)
  • Inactivating mutations of the retinoblastoma gene (RB) are found in a wide variety of tumour cells. (wdv.com)
  • RNA was extracted from colon for DNA repair, apoptosis, cell cycle, antiinflammation, proto-oncogene, colorectal cancer biomarker and tumor suppressor gene expressions study. (upm.edu.my)
  • The colon gene expression analysis showed that vanillin could enhance recombinational repair and mismatch repair, arrest cell at cell cycle checkpoints, increase the expression of tumour suppressor gene, colorectal cancer biomarker and proto-oncogene. (upm.edu.my)
  • Expression of a reporter gene linked to the −101HCK promoter region was up-regulated by Sp1, but not by other members of the Sp1 family of transcription factors, in Drosophila Schneider cells. (uni-frankfurt.de)
  • immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
  • These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
  • Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
  • This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
  • Additionally, telmisartan increased the level of caspase-cleaved cytokeratin 18 (cCK18), partially contributed to the induction of apoptosis in HLF cells and reduced the phosphorylation of ErbB3 in HLF cells. (bvsalud.org)
  • Dmp1 is a unique tumor suppressor that activates Arf expression and thus induces p53-dependent cell cycle arrest. (eurekalert.org)
  • Octyl gallate reduces ATP levels and Ki67 expression leading HepG2 cells to cell cycle arrest and mitochondria-mediated apoptosis. (harvard.edu)
  • Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
  • In this study, we further investigated the mechanism of PA-induced p27 expression in prostate cancer cells (LNCaP, androgen-independent LNCaP [AIDL] and PC-3). (iiarjournals.org)
  • Our results suggest that PA attenuated Skp2 expression, thereby inhibiting ubiquitination and promoting p27 accumulation in all three prostate cancer cell lines. (iiarjournals.org)
  • We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (who.int)
  • Forced BCL-2 or BCL-XL expression reduced priming and resulted in survival and cell cycle arrest. (nih.gov)
  • In this study, we evaluated the effects of telmisartan on human CCA cells and to assess the expression of microRNAs (miRNAs). (bvsalud.org)
  • Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
  • Some alterations were restored when the cells were transferred back to serum-containing medium, indicating that expression of these genes was controlled by components in serum. (lu.se)
  • B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
  • Here, we show that in human epithelial cells this arises from the orientation of cortical flows at AJs. (ibecbarcelona.eu)
  • A battery of toxicity assays was performed using human bronchial epithelial cells (BEAS-2B) and human THP-1 monocytes in doses ranging from 1-100 µg/ml. (cdc.gov)
  • Site-directed mutagenesis of the proximal promoter region showed that two GC-rich sequence elements are essential for transcriptional activity in myeloid cells. (uni-frankfurt.de)
  • Dihazi GH, Bibi A, Jahn O, Nolte J, Mueller GA, Engel W, Dihazi H. Impact of the antiproliferative agent ciclopirox olamine treatment on stem cells proteome. (wjgnet.com)
  • Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maGSCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA), which is known to have a differentiating effect on stem cells. (wjgnet.com)
  • Treatment of stem cells with CPX, a synthetic antifungal clinically used to treat superficial mycoses, resulted in an antiproliferative effect in vitro , without impairment of pluripotency. (wjgnet.com)
  • The antiproliferative effect of NBT was intercepted in GSK3β knockdown CCA cells. (ijbs.com)
  • The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
  • The mechanisms influenced by Flaoprirol and Lonafarnib are relevant on ovarian cancer cells. (uni-marburg.de)
  • Although SAMHD1 is expressed ubiquitously throughout the human body, the molecular mechanisms regulating its enzymatic activity and function in non-immune cells are relatively unexplored. (oncotarget.com)
  • His group studies the physical and molecular mechanisms by which cells detect and respond to mechanical signals. (ibecbarcelona.eu)
  • Whereas the mechanisms activating mechanotransduction are well studied, the reversibility of this process, whereby cells disassemble and reverse force-activated signalling pathways upon cessation of mechanical stimulation is far less understood. (ibecbarcelona.eu)
  • This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
  • Casticin, a natural polymethoxyflavone isolated from A. annua, V. trifolia, and V. agnus‑castus induces apoptosis in cancer cells by activating FoxO3a. (spandidos-publications.com)
  • The goal of the current study was to conduct a comparative toxicity study of representative 2D materials for the different categories listed above using high throughput in vitro screening assays. (cdc.gov)
  • The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)
  • We hypothesized that high mitochondrial priming prior to p53 reactivation would lead to apoptosis, while low priming would lead to survival and cell cycle arrest. (nih.gov)
  • Some researchers have recently proved in vitro, the antitumoral activity of this molecule against lung cancer cells with apoptosis and cell cycle arrest. (alliedacademies.org)
  • We have obtained interesting result in vitro, using A549 lung cancer cells line, that prove that allylic double bond is not the most important driving force, but other factors like substituents or the position of the unsaturation site can affect the activity. (alliedacademies.org)
  • The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
  • In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
  • Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
  • Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
  • The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
  • This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)
  • We have demonstrated that phenylacetate(PA)-induced cell cycle arrest in human prostate cancer is mediated by increase of p27. (iiarjournals.org)
  • Apigenin induces autophagic cell death in human papillary thyroid carcinoma BCPAP cells. (spandidos-publications.com)
  • The objective of this study is to evaluate the antioxidant and anticancer effects of ethanol extract of S. rufopilosa (EESR) and to determine the molecular mechanism of its anticancer activity in human colon carcinoma HT29 cells. (jcpjournal.org)
  • We assessed the effects of telmisartan on human PDAC cells using the cell lines PK-1 and PANC-1. (bvsalud.org)
  • SKOV-3 and BG1 were used as ovarian cancer cell lines. (uni-marburg.de)
  • Their main role is in cell-signaling, and, because they are hydrophobic, their main actions are limited to paracrine (cell-to-cell) or autocrine (same cell), rather than systemic, effects. (medscape.com)
  • The effects of VALD-3 on the cell cycle were analyzed in MCF-7 and MDA-MB-231 cells by PI single staining. (researchsquare.com)
  • After 14 weeks of vanillin administration, the effects of vanillin on blood cells, kidney, liver and brain were studied. (upm.edu.my)
  • This shows that 5% ethanol could pose negative effects onto the brain cells. (upm.edu.my)
  • We studied the effects of telmisartan on CCA cells using two cell lines, HuCCT-1 and TFK-1. (bvsalud.org)
  • We studied the effects of telmisartan on HCC cells using the HLF, HLE, HepG2, HuH-7 and PLC/PRF/5 cell lines. (bvsalud.org)
  • Additionally, in-vitro experiments, indicated that OEO/thymol interacts with DNA via minor grooves confirmed by docking method. (irost.org)
  • JDP2 induces cell cycle arrest through cyclin D, p53, and cyclin A transcription, by increasing JUNB, JUND, and Fra2, and by decreasing c-JUN through the loss of p27kip1. (wikipedia.org)
  • Flavopiriol blocks the ATP-binding site of cyclin-dependent cinases and leads to a G1- respectively G2-cellcycle arrest in vitro. (uni-marburg.de)
  • 8 , 9 Cell cycle program is regulated by cyclin/cyclin-dependent kinase (CDK) complexes. (jcpjournal.org)
  • In-vitro-treatment with Lonafarnib leads to a dose-dependent G2-arrest of glioblastomacells, lungcancer- and fibrosarcomacells. (uni-marburg.de)
  • Morphological changes, increased level of intracellular level of reactive oxygen species and loss of mitochondrial transmembrane potential of keratin-forming tumor cell line cells were evidently visualized on staining. (ijpsonline.com)
  • In vitro, both peptides were highly active against intracellular Leishmania major in mouse macrophages without exerting toxicity in host cells. (unav.edu)
  • Treatment of neuroblastoma PDX cells with ARRY-520 resulted in mitotic arrest and subsequent cell death. (lu.se)
  • These findings reveal a novel mechanism for regulating AMPK/FoxO3a signaling in response to casticin, suggesting a new strategy for SCLC therapy by targeting cancer stem‑like cells. (spandidos-publications.com)
  • Findings from the in vitro study showed that vanillin was cytotoxic towards HT-29 and 3T3 cells with the IC50 value of 400 μg/ml and 1000 μg/ml respectively. (upm.edu.my)
  • In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
  • Clinical trials have shown that patients with prostate, lung, and basal cell carcinoma have benefited from treatment with itraconazole, and there are additional reports of activity in leukaemia, ovarian, breast, and pancreatic cancers. (ecancer.org)
  • One such input is mechanical force, which activates signalling and regulates cell behaviour in the process of mechanotransduction. (ibecbarcelona.eu)
  • Therefore, cells must be equipped with molecular tools to adapt and respond to constantly fluctuating inputs. (ibecbarcelona.eu)
  • Taken together, our study demonstrated the therapeutic potential of telmisartan and provides molecular mechanistic insights into its anti-tumor effect on PDAC cells. (bvsalud.org)