Vitro adult phenotype cardiomyocytes. Medical search. Frequent questions

One of the first specialized cell types to be generated from hPSC was cardiomyocytes (CM), and differentiation protocols have evolved over the years and now allow for robust and large-scale production of hPSC-CM. Still, scientists are struggling to achieve the same, mainly ventricular, phenotype of the hPSC-CM in vitro as their adult counterpart in vivo. (bvsalud.org)
In this review, we compare the in vivo development of cardiomyocytes to the in vitro differentiation of hPSC into CM with focus on electrophysiology, structure and contractility. (bvsalud.org)
Metabolic changes were characterised in undifferentiated CDCs and during differentiation towards a cardiac phenotype. (ox.ac.uk)
Collagen IV promoted proliferation of CDCs whereas fibronectin primed cells for differentiation towards a cardiac phenotype. (ox.ac.uk)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
Moreover, hEGFR KI/KI mice display accelerated chondrocyte and osteoblast differentiation, a phenotype that is also present in Egfr -/- mice and has not been previously described. (silverchair.com)

The presence of cardiomyocyte MPO was associated with higher chlorination and peroxidation activity, increased levels of 3-chlorotyrosine-modified cardiac myosin binding protein-C (MYBPC3), attenuated phosphorylation of MYBPC3 at Ser-282, perturbed calcium signalling, and impaired cardiomyocyte relaxation. (bvsalud.org)
Cardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. (bvsalud.org)
Cardiosphere-derived cells (CDCs) can be expanded in vitro and induced to differentiate along the cardiac lineage. (ox.ac.uk)
Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
Recently, EndMT has been increasingly recognized as a vital process that contributes to various cardiovascular pathologies in adults, which include atherosclerosis, valvular heart disease, cardiac fibrosis, and myocardial infarction. (dovepress.com)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
Direct cardiac reprogramming of fibroblasts is an attractive therapeutic strategy for treating cardiovascular diseases because the adult heart has a low regeneration capacity [ 1 ]. (thno.org)
However, cardiac reprogramming efficiency is lower in vitro than in vivo [ 4 , 5 , 20 , 21 ]. (thno.org)
This suggests that the natural cardiac milieu may be a critical factor for direct cardiac reprogramming [ 2 , 14 , 22 , 23 ], and delivery of cues in the cardiac microenvironment to fibroblasts may improve the efficiency of direct cardiac reprogramming in vitro . (thno.org)
Amend N, Thiermann H, Worek F, Wille T ( 2019 ) The arrhythmogenic potential of nerve agents and a cardiac safety profile of antidotes - A proof-of-concept study using human induced pluripotent stem cells derived cardiomyocytes (hiPSC-CM). Toxicol Lett 308:1-6. (multichannelsystems.com)
Oleaga C, Jalilvand G, Legters G, Martin C, Ekman G, McAleer CW, Long CJ, Hickman JJ ( 2019 ) A human in vitro platform for the evaluation of pharmacology strategies in cardiac ischemia. (multichannelsystems.com)

More than ever before, we are able to visualize and analyze human physiological processes, gathering data in vivo, ex vivo, and in vitro using advanced imaging technologies and discovering previously unrealized patterns and connections using artificial intelligence and other computational tools. (corning.com)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
Compared with the control group, the expression of NOX2, nuclear factor-κB (NF-κB), and Snail was increased in vivo and in vitro but decreased with TMZ treatment. (dovepress.com)

In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)

The enormous variants and mutations found mean that we are still far from being able to concretely clarify a genotype-phenotype relationship. (bvsalud.org)

Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)

Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
An in vitro model of EndMT was developed using human umbilical vein endothelial cells (HUVECs) via treatment with transforming growth factor-β (TGF-β) at 10 ng/mL for 24 h. (dovepress.com)
2 EndMT is a process by which endothelial cells lose a portion of their cellular features and obtain the mesenchymal phenotype, including the loss of tight junctions and increased production of extracellular matrix. (dovepress.com)
In present study we characterized human brain endothelial cells, hCMEC/D3, which are widely utilized as BBB in vitro model. (helsinki.fi)

METHODS AND RESULTS: Human cardiomyocytes derived from control-induced pluripotent stem cells (iPSC-CMs) were shown to express MPO, with MPO levels being increased in iPSC-CMs generated from two HCM patients harbouring sarcomeric mutations in the MYBPC3 and MYH7 genes. (bvsalud.org)
Furthermore, known epigenetic changes underlying the differences between adult human CM and CM differentiated from pluripotent stem cells are described. (bvsalud.org)
This should provide the reader with an extensive overview of the current status of human stem cell-derived cardiomyocyte phenotype and function. (bvsalud.org)
He has been appointed as the Coordinator of various pan-European consortia including the large-scale integrated EU-projects FunGenES ("Functional Genomics of Engineered Embryonic Stem Cells), CRYSTAL ("Cryo-banking of stem cells for human Therapeutic Application"), ESNATS ("Embryonic stem cell-based Novel Alternative Testing Strategies"), and DETECTIVE - Detection of endpoints and biomarkers for repeated dose toxicity using in vitro systems. (wikipedia.org)
He was the first scientist worldwide to perform electrophysiological and functional experiments on stem cells and differentiated phenotypes thereof. (wikipedia.org)
He achieved a first functional characterization of cardiomyocytes developed from embryonic stem cells which is an important prerequisite for their subsequent use in regenerative medicine. (wikipedia.org)
In his current position, as Chairman and Director to the Institute for Neurophysiology at the University of Cologne, his research areas include work on embryonic and adult stem cells, induced pluripotent stem cells, mesenchymal stem cells and germline pluripotent stem cells. (wikipedia.org)
As a result of over five decades of investigation, mesenchymal stromal/stem cells (MSCs) have emerged as a versatile and frequently utilized cell source in the fields of … MSC have been shown to differ-entiate in vitro into adipocytes, chon-drocytes, osteoblasts, myocytes, and ß-pancreatic islets cells. (tomeipowered.com)
The term 'mesenchymal stem cells' (MSCs) is most commonly used to describe multipotent self-renewing cells that can be differentiated in vitro to generate adipocytes, chondrocytes, and osteoblasts. (tomeipowered.com)
Mesenchymal stem cells (MSCs) are an example of tissue or 'adult' stem cells. (tomeipowered.com)
Current methods for the maturation of induced pluripotent stem cell-derived cardiomyocytes. (wjgnet.com)
Our objective is to produce a cell-based test for LQTS with induced pluripotent stem (iPS) cell technology, which allows adult cells to be "reprogrammed" to be stem cell-like cells. (ca.gov)
Adult stem cells turn out to be a more robust and reliable source of cell to heal damaged cells. (drcremers.com)
These adult stem cells, such as from fat (adipose derived) or bone marrow, have the ability to transform into different cells. (drcremers.com)
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. (drcremers.com)
To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro . (drcremers.com)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

These iPS cells differentiate into beating cardiomyocytes. (ca.gov)
Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
Growing evidence suggests that transcriptional regulators and secreted RNA molecules encapsulated within membrane vesicles modify the phenotype of target cells. (unicyte.ch)
The cell phenotype is therefore determined by signals that target the cells received within a defined microenvironment. (unicyte.ch)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Therefore, the implantation of human cells reprogrammed in vitro represents an alternative strategy [ 16 - 19 ]. (thno.org)
In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
Theoretically, cells expressing high ACE2 levels are more vulnerable to SARS-CoV-2 invasion and subsequent organ injury, including cardiomyocytes (CMs). (biomedcentral.com)
The severity of the phenotypes correlates with the expression levels of the hEGFR KI allele, which is not efficiently expressed in epithelial and bone cells, but is expressed at similar and even higher levels as the endogenous Egfr in brain and heart. (silverchair.com)

Supported by epidemiological data and experimental studies, specific mechanisms have been defined linking environmental perturbations, disrupted fetal and neonatal development and adult ill-health. (mdpi.com)
Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
Tahrir FG, Gordon J, Feldman AM, Cheung J, Khalili K, Mohseni Ahooyi T ( 2019 ) Evidence for the impact of BAG3 on electrophysiological activity of primary culture of neonatal cardiomyocytes. (multichannelsystems.com)

In vitro CSC-derived cardiomyocytes exhibit the typical microRNA-mRNA blueprint of endogenous cardiomyocytes Communications Biology volume 4, Article number: 1146 (2021) Cite this article. (unicz.it)
Superior specificity is achieved by combining Cre-mediated AAVR overexpression with whole-body knockout of endogenous Aavr , which is demonstrated in heart cardiomyocytes, liver hepatocytes and cholinergic neurons. (nature.com)

Omicron's R0 could be as high as 10, indicating a more contagious phenotype than earlier virus variants. (biomedcentral.com)

Finally, we found that MPO protein was expressed in healthy adult murine and human cardiomyocytes, and MPO levels were increased in diseased hearts with left ventricular hypertrophy. (bvsalud.org)

Additionally, DRAM2 overexpression promoted cell metastasis and proliferation in vitro, while knockdown of DRAM2 expression yielded opposite result. (cancerindex.org)

Additionally, the reader will gain insight into the underlying signaling pathways and mechanisms responsible for cardiomyocyte development. (bvsalud.org)
Identification of novel AC9 binding partners, including the small heat shock protein 20 (Hsp20) and Popeye domain containing (Popdc) proteins may contribute to the underlying mechanisms of AC9 -/- phenotypes. (tmc.edu)
However, the relevant pathogenetic mechanisms of myocardial injury caused by direct viral invasion of cardiomyocytes have not been fully elucidated. (biomedcentral.com)

The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

Kraushaar U, Guenther E ( 2019 ) Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes Using Multiwell Microelectrode Arrays. (multichannelsystems.com)

To recapitulate the phenotype of an adult cardiomyocyte, differentiating progenitors need to upregulate mitochondrial glucose and fatty acid oxidation. (ox.ac.uk)

AIMS: Hypertrophic cardiomyopathy (HCM) is characterized by cardiomyocyte hypertrophy and disarray, and myocardial stiffness due to interstitial fibrosis, which result in impaired left ventricular filling and diastolic dysfunction. (bvsalud.org)
Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)

Nobel Prize winner Alexis Carrel performed numerous experiments clearly showing that tissue explants, including connective tissue and heart tissue, could be cultured in vitro preserving their characteristics for prolonged periods of time [ 2 ] supporting the notion that entire organs could be cultured in vitro. (intechopen.com)
ClinGen definitively disease-associated inherited heart disease genes were amplified using RNA extracted from fresh blood, derived cardiomyocytes, and myectomy tissue. (cdc.gov)

To clarify conflicting reports for AC9 regulation, proposed regulators were systematically evaluated, including G-proteins, protein kinases, and forskolin utilizing in vitro and cell based assays. (tmc.edu)
However, in whole blood, opsonophago- are its exopolysaccharide capsule and the surface-associat- cytic and intracellular killing assays, the LH/HC phenotype ed toxin, -hemolysin/cytolysin (-h/c). (cdc.gov)

The long-standing paradigm of the heart as a non-regenerative organ has finally been replaced, with our group contribution, by a wealth of data showing that new cardiomyocyte (CMs) are formed in the adult mammalian heart. (unicz.it)
Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)

These findings highlight cardiomyocyte MPO as a novel therapeutic target for improving myocardial relaxation associated with HCM, a treatment strategy which can be readily investigated in the clinical setting, given that MPO inhibitors are already available for clinical testing. (bvsalud.org)

These may now represent novel therapeutic targets against deleterious functions of CFs in adult cardiovascular disease. (bvsalud.org)

Bex1 knockout mice present ciliopathy phenotypes and exhibit ciliary defects in the retina and striatum. (biomedcentral.com)

Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)

Fetal and adult CFs influence neighboring cardiomyocytes in different ways. (bvsalud.org)
In vitro generated cardiomyocytes are generally described as fetal-like rather than adult. (bvsalud.org)

Juvenile animals possess physiological characteristics distinct from those of adults. (biomedcentral.com)

Limited by insufficient evidence, myocardial injury caused by direct viral invasion of cardiomyocytes (CMs) is not fully understood. (biomedcentral.com)

Using a genome-wide approach, we identified and validated 2 crucial players for maintaining the adult primary human CF phenotype. (bvsalud.org)

Here, we investigated whether myeloperoxidase (MPO) is expressed in cardiomyocytes and provides a novel therapeutic target for alleviating diastolic dysfunction in HCM. (bvsalud.org)

A pig kidney in vitro perfusion model was established. (gu.se)

As adults, our health can be influenced by a range of lifestyle and environmental factors, increasing the risk for developing a series of non-communicable diseases such as type 2 diabetes, heart disease and obesity. (mdpi.com)
Over the past few decades, our understanding of how our adult health can be shaped by events occurring before birth has developed into a well-supported concept, the Developmental Origins of Health and Disease (DOHaD). (mdpi.com)
The incidence of linked to expression of an orange pigment with antioxidant invasive GBS disease in nonpregnant adults is growing, in properties ( 6 , 15 ), and these 2 factors act in concert to im- particular in elderly persons and in those with chronic un- pair macrophage-based immune clearance ( 16 ). (cdc.gov)

Blood group phenotypes relating to expression of ABO antigens in individual kidneys were identified. (gu.se)

Risk perception and intended behavior change after uninformative genetic results for adult-onset hereditary conditions in unselected patients. (cdc.gov)

Our aim is to investigate how aging alters synapses and how synaptic disruption contributes to the development of neurodegeneration-like phenotypes upon aging. (uni-ulm.de)

Overall, I conclude that most G-proteins or protein kinases do not directly regulate AC9, except Gαs, in vitro. (tmc.edu)

Of the AC isoforms present in adult cardiomyocytes (AC 4, 5, 6, and 9), AC9 is the most divergent in sequence and understudied. (tmc.edu)

Referenced article focusing on the relationship between phenotype and genotype. (cancerindex.org)

Different studies have shown a different prognosis in T2DM patients and T2DM elderly patients treated by CRT-D. We reviewed the literature data on CRT-D effect on adult and elderly patients with T2DM as compared with nondiabetic patients. (hindawi.com)

Anatomy29

Organisms15

Diseases17

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes67

Disciplines and Occupations1

Information Science3

Differentiation8

Cardiac15

Vivo3

Fibroblasts2

Genotype-phenotype1

Scaffolds1

Endothelial cells4

Stem18

Cells10

Neonatal3

Endogenous2

Variants1

Murine1

Knockdown1

Mechanisms3

Transplantation1

20191

Progenitors1

Ventricular2

Tissue2

Assays2

Regenerative2

Clinical1

Cardiovascular disease1

Exhibit1

Cell2

Fetal2

Distinct1

Myocardial1

Human1

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Model1

Disease3

Expression1

Behavior1

Contributes1

Protein1

Present1

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Studies1