Vitrification embryo mammalian. Medical search. Frequent questions

Various factors important for successful vitrification of bovine and equine oocytes were addressed. (wur.nl)
Illustrative example of progress made is the world-first foal born in 2017 after development of an improved vitrification protocol for equine immature oocytes. (wur.nl)
Embryonic stem cells (ESCs) from the 'inner cell mass' of an early embryo give rise to all future cells and tissue of an animal, including the primordial germ cells, which ultimately form the germ cells (sperm or oocytes). (wur.nl)
Retrospectively, it seems absurd, but the respected big-bearded academics disregarded the obvious fact that - unlike those of frogs and salamanders - oocytes and embryos of mammals are extremely sensitive to temperature changes and do not tolerate exposition to room temperature, not even for a short period. (vitavitro.com)
Levels of maturation-promoting factor (MPF) in oocytes decline after vitrification, and this decline has been suggested as one of the main causes of low developmental competence resulting from cryoinjury. (molcells.org)
Cryopreservation of mammalian oocytes has been successfully applied for long-term storage of surplus eggs in mammalian assisted reproductive technology (ART), including in humans. (molcells.org)
She has taken extensive training in vitrification of oocytes and embryos at the Kato Ladies Clinic, Tokyo. (iwannagetpregnant.com)
Her skills include vitrification and thawing of oocytes and embryos. (iwannagetpregnant.com)
Anjali's areas of interest are: Sperm morphological and funtional parameters, Extended culture , Assisted Hatching , Blastomere and trophectoderm biopsy , PGD , Vitrification of Embryos and Oocytes and Oocyte Banking. (iwannagetpregnant.com)

Dr. Henri Woelders, of Wageningen Livestock Research (WLR) and the Centre for Genetic Resources, the Netherlands (CGN) presented on the potential of cryopreservation and use of various types of germplasm in pigs and chicken, focussing on embryos, gonads, primordial germ cells and semen, and including some highlights from the EU Horizon 2020 IMAGE project (LINK). (wur.nl)
Cryopreservation and transfer of gonads was shown to be an effective means in bird and mammalian species, while primordial germ cells offer possibilities and potential advantages in bird and fish species. (wur.nl)
Also, recent advances in pig embryo cryopreservation were shown and it was demonstrated how mathematic modelling of cellular events during embryo cryopreservation are extremely helpful. (wur.nl)
Oocyte vitrification (cryopreservation) has the potential to rescue gametes from deceased animals, it would make oocyte banking for research possible, and superior female genetics could be cryopreserved. (wur.nl)
Utrecht can do ovum pick-up from mares and collaborate with Avantea, Italy for in vitro production and cryopreservation of embryos. (wur.nl)
From 21 to 25 October 2013 the CNR Monterotondo and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. (infrafrontier.eu)
The course is designed primarily as a hands-on laboratory program in which participants learn techniques for the cryopreservation of cleavage stage embryos, spermatozoa and ovaries. (infrafrontier.eu)
Vitrification, an alternative cryopreservation tool, has recently been shown to prevent ice formation and involves the use of a high concentration of CPAs and extremely high cooling rate. (molcells.org)

Human oocyte vitrification works very well, further work on oocyte vitrification in cattle, horse, cat can learn from human. (wur.nl)
Another drawback of oocyte vitrification is diminished developmental potential because of the decreased quality of embryos after warming. (molcells.org)

He and his team described the biological processes related to reproductive cyclicity, sperm, egg and embryo development in carnivores, especially felids and canids. (si.edu)
In vitro fertilization (IVF) involves combining an egg and sperm within a laboratory setting to create an embryo. (ivf.net)

Mammalian embryology had a very bad start. (vitavitro.com)
That was the time when mammalian embryology was born. (vitavitro.com)
He also delivered keynote lectures at prestigious conferences including the International Embryo Transfer Society, European Society of Human Reproduction and Embryology, and American Society of Reproductive Medicine. (si.edu)

11 Among women undergoing in vitro fertilization-embryo transfer in the United States, deliveries per oocyte retrieved decreased from 36.9 percent among women under the age of 35 years to 20.5 percent among women between the ages of 38 and 40 years and to 10.7 percent among women between the ages of 41 and 42 years. (ldysinger.com)

She was associated with various research projects in collaboration with the prestigious NIRRH, in Mammalian Reproductive Physiology. (iwannagetpregnant.com)
Embryo transfer (ET) is an essential reproductive technology for the production of new animal strains and maintenance of genetic resources. (nature.com)
Despite the limitations, embryo transfer and related reproductive technologies hold promise as a means to accelerate genetic improvement and to take advantage of superior breeding sows, to break the link between health status of customer and source herds, to eradicate disease from a swine herd, and to facilitate inexpensive transport of genetic material around the world. (veteriankey.com)

From the opening of the book the reader is lead on a fantastic voyage from the formation of the primordial oocyte to the development of the early embryo, passing through crucial processes of oogenesis, such as co-ordination of oocyte and follicle growth, gene expression and organelle reorganization during growth and maturation, epigenetic mechanisms, regulation of meiosis, totipotency, cell polarity, oogenesis in vitro and maternal regulation of early development. (ivf.net)

Furthermore, high developmental rates of offspring were observed when pronuclear and two-cell embryos were transferred to females in estrus that were stimulated on the day of embryo transfer. (nature.com)

An optimal temperature for humans is devastating to cattle and pig embryos and vice versa, although the difference is an almost negligible 1.5 to 2.0 degrees. (vitavitro.com)
Can we make superintelligent humans by repeatedly selecting embryos in a test tube? (debunkingdenialism.com)

Prof. Ann van Soom of the Faculty of Veterinary Medicine, Ghent University, presented an overview of the major progress made in the past years in vitrification, oocyte and embryo culture in horses, cats and cattle. (wur.nl)

Offspring were obtained from two-cell embryos transferred into females with pseudopregnancy induced using sonic vibration in proestrus on the day before embryo transfer. (nature.com)
Genome-edited mice were also obtained using frozen-warmed pronuclear embryos with clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) nucleases introduced using the technique for animal knockout system by electroporation (TAKE) method, which were transferred to females with pseudopregnancy induced on the day of embryo transfer. (nature.com)
The induction of pseudopregnancy is required to transfer embryos produced under in vitro conditions into the oviducts of females. (nature.com)
In this study, estrus females were stimulated on the day of embryo transfer. (nature.com)
1 Until now, however, the swine industry has never successfully implemented a commercial viable embryo transfer process as an alternative method of improving hog genetics. (veteriankey.com)

They are also seriously affected by seemingly slight differences between the core temperatures of various mammalian species. (vitavitro.com)

Table 1 shows the development of embryos transferred to females with pseudopregnancy induced via sonic vibration. (nature.com)
Embryo development using artificially stimulated females was significantly lower than that of the control group in which pseudopregnancy was induced in females by mating with vasectomized males. (nature.com)
According to our findings, apoptosis seems to be the most frequently observed type of PCD, but it is not the exclusive type of morphological cell death during the development of axial structures in human embryos. (karger.com)

In the fifties of the last century, we had already the nuclear bomb, the sputnik, the television, the first digital computers - but not a single mammalian embryo produced in vitro . (vitavitro.com)

We analyzed the morphological features of dying cells in the developing axial structures of 5 human embryos between 5 and 8 weeks of postovulatory age. (karger.com)

Clarke, P.G.H. (1984) Identical populations of phagocytes and dying neurons revealed by intravascularly injected horseradish peroxidase, and by endogenous glutaraldehyde-resistant acid phosphatase in the brains of chick embryos. (karger.com)
γ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter in the mammalian central nervous system. (riken.jp)

Of all two-cell embryos transferred to these females with pseudopregnancy, 21.8% were implanted, and 12.7% of embryos developed into offspring. (nature.com)
Chu-Wang, I.W., R.W. Oppenheim (1978) Cell death of motoneurons in the chick embryo spinal cord. (karger.com)
The oocyte is the largest and most mysterious cell in the body of mammalian organisms. (ivf.net)

Because of the large numbers, this work provides insights into factors affecting success rates of ovum pick-up and in vitro embryo production. (wur.nl)
Healthy offspring from the IVP embryos is being obtained (with increasing success rates through the years). (wur.nl)

Choice of vitrification mode for macroporous matrices seeded with mesenchymal stromal cells. (in.ua)

Worse, however, is the mischaracterization of heritability as an objective context-free measure of the importance of genes and the biological ignorance about e. g. antagonistic pleiotropy and missing heritability underlying his discussion of iterated embryo selection for IQ. (debunkingdenialism.com)

Today, if you ask a primary school student what the most important thing to protect a mammalian embryo to avoid damages outside the body is, I am sure he would say: to keep it warm. (vitavitro.com)

Traditional slow-rate freezing was efficient for cleavage, precompaction stage human embryos, but in vitro survival and in vivo developmental rates were compromised after blastocyst freezing. (medscape.com)
6. Assessment of the optimal vitrification protocol for pre-pubertal mice testes leading to successful in vitro production of flagellated spermatozoa. (nih.gov)

For safety purposes, high cryoprotectant concentrations with potential toxicity are always referred, disregarding the fact that the final intracytoplasmic concentration of cryoprotectants is much higher in traditional freezing than in vitrification. (medscape.com)
Different types of cryoprotectants are used for vitrification protocols, including ethylene glycol (EG), dimethyl sulfoxide (DMSO), and 1, 2-propanediol (PrOH). (ijfs.ir)
Intracellular cryoprotectants permeate the cell membrane to effectively minimize cell damage, whereas extracellular cryoprotectants work by inducing vitrification (the solidification of water due to increased viscosity rather than crystallization), which does not have the same biologically damaging effects as freezing. (labmanager.com)

In conclusion, a caffeine treatment at 1.25 mM during delayed activation for 4 h can improve the preimplantation development of porcine somatic cell nuclear transfer embryos by activating nuclear reprogramming. (koreamed.org)

We analyzed publicly available single-cell RNA-seq datasets of human embryos for the known canonical and non-canonical receptors and spike protein cleavage enzymes for multiple coronaviruses like SARS-CoV, SARS-CoV-2, MERS-CoV, hCoV-229E, and hCoV-NL63. (nih.gov)
About 70% of human cleavage stage embryos show chromosomal mosaicism, falling to 20% in blastocysts. (elifesciences.org)

15. Comparison of cryosurvival and spermatogenesis efficiency of cryopreserved neonatal mouse testicular tissue between three vitrification protocols and controlled-rate freezing. (nih.gov)

Some companies and researchers even advertise their 'nontoxic', that is, DMSO-free, vitrification systems, although DMSO is probably the least toxic and most protective cryoprotectant compared with other commonly used permeable ones, such as propylene glycol. (medscape.com)

16. Spermaurin, an La1-like peptide from the venom of the scorpion Scorpio maurus palmatus, improves sperm motility and fertilization in different mammalian species. (nih.gov)
The amount of proAKAP4 reflects the ability of spermatozoa to maintain the flagellum activity and functionality up to the site of fertilization and is positively correlated with progressive motility in several mammalian specie. (researchgate.net)

Vitrification is a practical method that produces a glass-like solidification of the cells by rapid cooling and high concentrations of cryoprotective agents (CPAs). (ijfs.ir)

He is well known for his contributions to oocyte vitrification and the development of the largest donor oocyte cryo-bank in North America. (ivf.net)
The aim of this study was to test the embryo donor potential of cloned mares, and to compare the results obtained fro. (researchgate.net)

found a quite similar but statistically significant 6% TE enrichment in mouse embryos treated with the SAC inhibitor reversine to induce mosaic aneuploidy. (elifesciences.org)

Embryos from these banks can remain dormant for centuries, creating a reservoir of genetic diversity. (bvsalud.org)

[ 16-19 ] Finally, the theoretical danger of liquid nitrogen-mediated disease transmission is a most regrettable and absurd misinterpretation of scientific evidences, leading to fruitless arguments and the potential ban of the most efficient vitrification techniques (also discussed later). (medscape.com)
The resting membrane potential of large mammalian nerve fibers is about −90 milliVolts ( mV , negative inside the membrane), whereas the giant squid axon (which is an excellent model for experiments) has a membrane potential of −60 mV. (benbest.com)

The minimum amount of vitrification solution (~0.1 µl) remaining in Cryotop is in direct contact with liquid nitrogen during cooling. (ijfs.ir)
OPS has a small effect for reducing the volume of vitrification solution to 0.5 µl and thus increasing the cooling rate ( 18 ). (ijfs.ir)

Vitrification requires the use of cryoprotective agents ('antifreeze compounds') to prevent ice formation and/or freezing damage by increased viscosity, by hydrogen-bonding with water molecules, by dilution of electrolytes and/or by colligative interference. (benbest.com)

A great diversity of crustacean zooplankton found in inland and coastal waters produce embryos that settle into bottom sediments to form an egg bank. (bvsalud.org)

This hypothesis needs further study as we did not analyse chromosomal mosaic embryos. (elifesciences.org)
This study examined the effects of a caffeine treatment to improve nuclear reprogramming in porcine cloned embryos. (koreamed.org)

EG is a common CPA that is used for oocyte vitrification. (ijfs.ir)

Anatomy16

Organisms7

Diseases2

Chemicals and Drugs21

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes32

Information Science4

Health Care7

Oocytes9

Cryopreservation8

Oocyte vitrification2

Sperm2

Embryology3

Vitro fertilization1

Reproductive3

Maturation1

Developmental1

Humans2

Cattle1

Transfer5

Species1

Development3

NUCLEAR1

Human1

Acid2

Cell3

Success2

Cells1

Selection1

Important1

Vitro2

Cryoprotectants3

Preimplantation1

Cleavage2

Protocols1

DMSO1

Fertilization2

Concentrations1

Donor2

Mouse1

Genetic1

Potential2

Solution2

Damage1

Found1

Study2

Common1