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  • Receptors
  • To avoid this, we constructed CHO and PK15 cell lines recombinantly expressing the PRRSV receptors involved in virus entry into macrophages, sialoadhesin (Sn) and CD163 (CHOSn-CD163 and PK15Sn-CD163) and evaluated their potential for production of PRRSV. (nih.gov)
  • infectious virus
  • Second, VSV was incubated in LC phases of either C(14)AO + D or DSCG for 4 h, and the concentration (titer) of infectious virus in the LC was determined by dilution into cell culture medium and subsequent inoculation of HeLa cells. (biomedsearch.com)
  • In studies involving three monkeys experimentally infected with Ebola virus (Reston strain), fever and other systemic signs of illness preceded detection of infectious virus in the pharynx by 2-4 days, in the nares by 5-10 days, in the conjunctivae by 5-6 days, and on anal swabs by 5-6 days (P. Jahrling, U.S. Army Medical Research Institute of Infectious Diseases, unpublished data, 1995). (cdc.gov)
  • molecular
  • Knowledge of the atomic structure of these viruses now provides a foundation for dissecting the antigenic properties of caliciviruses as well as providing insights into the molecular interactions that govern virus assembly and genome encapsidation. (sciencemag.org)
  • Exciting new insights into the molecular pathogenesis and host-virus interactions have been gained over the past few decades. (eurekaselect.com)
  • titer
  • The immunity produced in monkeys, as measured by the antibody titer developed, has no relation to the amount of virus inoculated. (rupress.org)
  • The subcutaneous inoculation of the culture virus into immune persons leads to a substantial increase of the serum antibody titer. (rupress.org)
  • Furthermore, the virus titer of all 5 PRRSV strains produced after passaging in PK15Sn-CD163 cells was similar to the virus titer of those strains produced in Marc-145 cells. (nih.gov)
  • occur
  • Animal viruses, in particular, can cause microscopic or macroscopic degenerative changes or abnormalities in host cells and in tissues called cytopathic effects, cytopathic effects may be lethal, but plaque formation from cell lysis does not always occur. (blogspot.com)
  • Artificial virus' that doesn't occur in nature and may have evolved from smallpox or cowpox virus. (paramedicsworld.com)
  • hemorrhagic
  • Lassa fever is a potentially severe viral hemorrhagic illness caused by Lassa virus (LASV). (cdc.gov)
  • The risk for person-to-person transmission of hemorrhagic fever viruses is highest during the latter stages of illness, which are characterized by vomiting, diarrhea, shock, and often hemorrhage. (cdc.gov)
  • or 3) worked in a laboratory or animal facility that handles hemorrhagic fever viruses. (cdc.gov)
  • vaccine virus
  • We have made ten attempts to cultivate vaccine virus in tissue extracts prepared according to the method described by Eagles and Kordi (4). (rupress.org)
  • Renal, testicular, and chick embryo extracts were employed with a dermal strain of vaccine virus and with the Levaditi strain of neuro-vaccine virus. (rupress.org)
  • Nevertheless, one cannot examine the results of our work recorded in the six tables without recognizing the fact that in the types of media used the presence of viable cells appears to be essential for the multiplication of vaccine virus. (rupress.org)
  • sequence
  • Analysis of the sequence of the structural proteins of original virus and virus grown for 5 passages on PK15Sn-CD163 cells showed either no amino acid (aa) changes (VR-2332 and 07V063), one aa (LV), two aa (08V194) or three aa (08V204) changes. (nih.gov)
  • Infections
  • Hu-man-immunodeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. (springer.com)
  • Borkowsky W, Krasinski K, Paul D, Moore T, Bebenroth D, Chandwani S (1987) Humanimmundeficiency-virus infections in infants negative for anti-HIV by enzyme-linked immunoassay. (springer.com)
  • vaccines
  • For analysis of humoral immunity, groups of five Balb/c mice were immunized at day 0 (1×) or day 0 and 14 (2×) with 10 μg of single inactivated vaccines or 20 μg of co-formulated INAC-RV-GP + INAC-RV-HC50 (10 μg each virus). (mglurpathway.com)
  • single stranded
  • Norwalk virus contains a single-stranded, positive-sense, RNA genome of about 7.7 kb ( 3 ) that is incorporated within a shell consisting of multiple copies of a single protein of ∼56.6 kD ( 3 , 5 ). (sciencemag.org)
  • experiments
  • Control experiments using cell lysates with equivalent protein concentrations but no virus did not perturb the uniform alignment of the LC. (biomedsearch.com)
  • Lassa
  • Nosocomial transmission of Lassa virus (LASV) is reported to be low when care for the index patient includes proper barrier nursing methods. (cdc.gov)
  • Nosocomial
  • although the risk and/or mode of nosocomial transmission differs for each of these viruses, the limited data do not permit clear distinctions. (cdc.gov)
  • widespread
  • The G1-H9N2 viruses are widespread and more likely affected in commercial poultry flocks with moderate clinical signs whereas, Y280 and Kr-p96323 are apparently circulating in natural reservoir hosts and more prevalent in their respective origin areas. (biomedcentral.com)
  • cell
  • The clonal cell lines suspended in suitable media are infected with any desired virus which replicates inside the multiplying cells. (blogspot.com)
  • One aim was to enhance HBV production of the cell line HepG2.2.15 by cultivation on microcarrier substrate. (hu-berlin.de)
  • hu-man T-cell lymphotropic virus type III (HTLV-III) embryopathie. (springer.com)
  • Using these approaches, we found that the LC containing C(14)AO + D caused inactivation of virus as well as cell death. (biomedsearch.com)
  • Susceptible cell lines for the production of porcine reproductive and respiratory syndrome virus by stable transfection of sialoadhesin and CD163. (nih.gov)
  • A PRRSV susceptible cell line was constructed that can grow virus to similar levels compared to currently available cell lines. (nih.gov)
  • For Marc-145 grown virus infection of CHOSn-CD163 cells, little difference was observed between different cell densities and days post seeding, although a density of 200 000 cells/mL and inoculation at 2 days post seeding seemed a little more efficient. (nih.gov)
  • Viruses can replicate very fast and may affect any metabolic and physiological function of the host cell. (eurekaselect.com)
  • Virus-host interaction is a complex phenomenon and often is virus- and host cell-specific. (eurekaselect.com)
  • body fluids
  • Wearing a chemical or fireman's mask, or even a surgical mask, may possibly offer some protection against contracting the disease, which is caught by inhalation of the virus or contact with a patient's blood or body fluids. (medjournal.com)
  • In Africa, transmission of VHF has been associated with reuse of unsterile needles and syringes and with provision of patient care without appropriate barrier precautions to prevent exposure to virus-containing blood and other body fluids (including vomitus, urine, and stool). (cdc.gov)
  • methods
  • Methods: The literature related to various aspects of virus-host interactions: new insights and advances in drug development was collected from several scientific research related databases such as Science Direct, Google Scholar, Scopus, PubMed, AGRICOLA, and Medline, etc. (eurekaselect.com)
  • Identify And Recognize The Methods Of Microbial Cultivation, Purification And Assay. (uaeu.ac.ae)
  • however
  • Infected plants have however, tested positive for Tobacco Mosaic Virus (TMV). (thcfarmer.com)
  • However, it is highly contagious with a very small number of viruses inhaled, and the chances of contracting the disease from close and prolonged contact with acutely ill patients shedding viruses is high. (medjournal.com)
  • However, it was observed that Marc-145 grown virus infected the cells more efficiently than macrophage grown virus. (nih.gov)
  • causes
  • Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses in the pig industry worldwide. (nih.gov)