• To overcome the inherent defects of antimicrobial therapy in patients with salmonellosis, here we introduce a versatile vesicle-based system for delivering antibiotics to target the illness-causative agent, Salmonella enterica serovar Typhimurium selectively. (nature.com)
  • Lack of AcrA also attenuated the ability of S. Typhimurium to infect INT-407 and RAW 264.7 cells \(in vitro\) as previously published for AcrB and TolC mutants. (bham.ac.uk)
  • The virulence defect of the mutants could not be rescued by addition of supernatant from an infection of INT-407 cells with SL1344 or addition of media conditioned by uninfected INT 407 cells. (bham.ac.uk)
  • However, addition of media conditioned by overnight growth of SL1344 was able to ameliorate the virulence defect of the mutants. (bham.ac.uk)
  • This suggests that AcrAB-TolC of SL1344 exports a factor/s required for virulence which the mutants are unable to export and that exogenous addition of this factor can restore the virulent phenotype. (bham.ac.uk)
  • 2005). However, the emergence of atypical F1 mutants fully virulent in humans and with natural heterogeneity to Y. pestis LcrV highlights the limits click here of the current rF1-V fusion vaccine (Quenee et al. (tie-2signaling.com)
  • AcrA of S. \(Typhimurium\) SL1344 was inactivated and the mutant phenotype characterised. (bham.ac.uk)
  • The AcrA mutant was hyper-susceptible to a range of antimicrobials and was more susceptible to some agents than strains lacking AcrB and TolC. (bham.ac.uk)
  • As we found a fabB overexpressing strain to partly mimic the biofilm defect of the fabR mutant, the effect of FabR on biofilms can be attributed at least partly to FabB, which plays a key role in UFA biosynthesis. (biomedcentral.com)
  • Furthermore, vaccination of mice with the ΔyscN mutant provided some level of protection against a s.c. challenge (the equivalent of ~90LD50) with the wild-type strain for even the group vaccinated with the lowest mutant dose. (tie-2signaling.com)
  • using live mutant Y. pestis vaccine strains (Okan et al. (tie-2signaling.com)
  • All the previously identified E. coli FabR transcriptional target genes ( fabA , fabB and yqfA ) were shown to be direct S. Typhimurium FabR targets as well. (biomedcentral.com)
  • However, in Brazil there are few studies that have conducted a virulence characterization of this serovar. (nih.gov)
  • In this work, we assess the ability of a promiscuous, constitutively-active bEBP-the AAA+ ATPase domain of DctD from Sinorhizobium meliloti -to activate transcription from all σ 54 -dependent promoters for the characterization of the σ 54 regulon of Salmonella Typhimurium LT2. (biomedcentral.com)
  • COE2-2hexyl effectively-treated mice infected with bacteria derived from sepsis patients with refractory bacteremia, including a CRE K. pneumoniae strain resistant to nearly all clinical antibiotics tested. (pharmaceuticalintelligence.com)
  • Since the DctD AAA+ ATPase domain proved effective in activating transcription from the diverse σ 54 -dependent promoters of the S . Typhimurium LT2 σ 54 regulon under a single growth condition, this approach is likely to be valuable for examining σ 54 regulons in other bacterial species. (biomedcentral.com)