Virulence factor expression gene expression regulation bacterial. Medical search. Frequent questions

Knockout mutants of rna01 and a double knockout mutant of rna01 and hfq, both had decreased biofilm formation and hemolytic activity, attenuation for virulence in Galleria mellonella, altered stress susceptibility, and an altered outer membrane protein profile. (bvsalud.org)
QS is a process of bacterial cell-cell communication that controls virulence and biofilm formation in many bacterial species. (wadsworth.org)
We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)
This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus . (hindawi.com)
This regulatory process manifests itself with a variety of phenotypes including biofilm formation and virulence factor production. (absin-reagent.com)

In this sense, we aim to characterize the behaviour of different promoters of genes involved in biofilm regulation under different conditions and to search for possible new DNAbinding proteins associated with their promoter regions. (usp.br)
100 types on the basis of immunogenic differences in their surface M proteins and polymorphisms in the emm gene ( 1 ). (cdc.gov)
In addition to the PrfA regulon, the Clp stress proteins have an impact on Listeria virulence. (ed.ac.uk)
Whereas regulating gene expression in multicellular organisms allows for cellular differentiation, in single-celled organisms like prokaryotes, it primarily ensures that a cell's resources are not wasted making proteins that the cell does not need at that time. (pressbooks.pub)
These thermosensors are part of a regulatory network, such as the production of heat shock proteins mediated by sigma factor 32 (RpoH) in Escherichia coli or the transcriptional repressor of heat-shock genes HrcA in Bacillus subtilis (Hecker et al. (springer.com)
Analysis of virulence related gene expression and computational simulations of pivotal proteins involved in pathogenesis demonstrate that myricetin downregulates the saeR global regulator and interacts with sortase A and α-hemolysin. (nature.com)
Two genes encoding putative internalin proteins were chosen for further study. (omictools.com)
Sigma factors are a class of proteins constituting essential dissociable subunits that confer sequence-specific DNA binding properties to RNA polymerase (RNAP). (hindawi.com)
At increased populations of the bacteria, localized higher concentrations of 3-O-C6-HSL, an endogenous ligand to transcriptional factor LuxR, leads to increased production of both the AHL synthase and proteins responsible for bioluminescence. (absin-reagent.com)

An important factor of bacterial virulence is the formation of biofilms which are aggregates of microorganisms incorporated into an extracellular matrix that protects bacteria from hostile environments. (usp.br)
Bacterial regulatory small RNAs (sRNAs) play important roles in gene regulation and are frequently connected to the expression of virulence factors in diverse bacteria. (bvsalud.org)
As in all pathogenic bacteria, virulence of the facultative intracellular Listeria species is a multifactorial trait. (ed.ac.uk)
Research in the Paczkowski laboratory focuses on the mechanisms of regulation of quorum sensing (QS) in Gram-negative bacteria, such as Pseudomonas aeruginosa , Vibrio cholerae , Aeromonas hydrophila , and Chromobacterium violaceum . (wadsworth.org)
QS allows bacteria to synchronously alter gene expression patterns that underpin collective behaviors. (wadsworth.org)
Learning how bacteria correctly interpret these blends of AIs and elicit appropriate gene expression responses is essential to understand how bacteria communicate, and, more globally, to understand how all organisms decode environmental stimuli. (wadsworth.org)
We use a combination of bacterial genetics, biochemistry, structural biology, and chemical biology to understand how bacteria respond appropriately to signals in a complex environment. (wadsworth.org)
Consequently, bacteria have developed different systems to sense changes in environmental conditions such as temperature and induce an adaptation of metabolism and gene expression. (springer.com)
Subsequently, the bacteria move to the xylem vessels of the plants to express their virulence factors. (ppjonline.org)
Delaying expression of by as little as 3?h enhanced clearance of bacteria and survival of infected. (phytid.org)
Inversely, high ID50 values are associated with motile and fast-growing bacteria that use quorum-sensing based regulation of virulence factors expression. (pasteur.fr)
Bacteria with variant cagY alleles that reduced T4SS function showed comparable reduction in binding to a5ß1 integrin, although CagY was still expressed on the bacterial surface. (pacb.com)
Given the high number of virulence factors present in these bacteria, and the niche-specific role many of them play during different stages of the infectious process, gene expression must be finely tuned in order to efficiently coordinate their expression, and also continue to preserve energy pools. (biomedcentral.com)
Besides the vegetative sigma factor, which recognizes the "standard" promoter, many bacteria possess alternative sigma factors that confer altered promoter specificity upon RNAP [ 2 ]. (hindawi.com)
N-(β-ketocaproyl)-L-Homoserine lactone is a component of quorum regulatory sensing which is a regulatory system used by bacteria for controlling gene expression in response to increasing cell density. (absin-reagent.com)
We will then work out how this causes changes in the production of the factors that affect where and how the bacteria colonise. (ukri.org)
They used a gene that encodes for 16S ribosomal RNA (rRNA) in bacteria to characterize the nucleotide sequence of the bacillus from a patient with Whipple disease. (medscape.com)

For example, in chronic skin wounds, Pseudomonas aeruginosa virulence is activated by Gram-positive organisms, such as Staphylococcus aureus . (asmblog.org)
Mapping transcriptional regulation of biofilm-related genes promoters in Pseudomonas. (usp.br)
The bacterial plant pathogen Pseudomonas syringae causes economically important diseases of a wide variety of plant species and is used as a model organism to understand the molecular basis of plant disease. (ox.ac.uk)
Pseudomonas aeruginosa is an adaptable bacterial pathogen that infects a variety of organs, including the respiratory tract, vascular system, urinary tract, and central nervous system, causing significant morbidity and mortality. (biomedcentral.com)

Pathogenic strains then produce two toxins (TcdA and TcdB), which are considered as the major virulence factors, that lead to the disruption of the actin cytoskeleton of intestinal epithelium cells (6,7), therefore, conferring the CDI symptoms (diarrhoea, epithelial apoptosis and ulceration). (studyres.com)

We show that GtrS and GltR form a two-component system that regulates the expression from the promoters Pedd/gap-1, PoprB and Pglk, which control the expression of genes involved in glucose metabolism and transport. (nih.gov)
We propose that 2-KG is a key metabolite in the stringent transcriptional control of genes involved in virulence and glucose metabolism. (nih.gov)
The latter group has several potential mRNA targets, many of which are involved with virulence, stress resistance, or metabolism, and connect the sRNAs in a complex gene regulatory network. (bvsalud.org)
The CcpA regulon comprises genes involved in sugar uptake, fermentation and amino acids metabolism, confirming the role of CcpA as a link between carbon and nitrogen pathways. (studyres.com)
Using model predictions, we designed and characterized 47 additional pathway variants to navigate its activity space, find optimal expression regions with desired activity response curves, and relieve rate-limiting steps in metabolism. (omictools.com)
Infections by bacterial pathogens generate substantial tissue damage and alteration in metabolism that have detrimental consequences even after infections have been cleared. (grantome.com)
Our recent work has focused on analysing the roles of both bacterial and host metabolism in affecting bacterial gene expression. (ukri.org)

The freshwater bacterial pathogens Edwardsiella ictaluri, Aeromonas hydrophila and Flavobacterium columnare infect a variety of farmed fish species worldwide through various mucosal attachment points. (auburn.edu)
The bacterial pathogens within the RSSC produce exopolysaccharide (EPS), a highly complicated nitrogen-containing heteropolymeric polysaccharide, as a major virulence factor. (ppjonline.org)
We mined the literature for experimental data on infectious dose of bacterial pathogens in humans (ID50) and also for traits with which ID50 might be associated. (pasteur.fr)
Streptococcal pathogens continue to evade concerted efforts to decipher clear-cut virulence mechanisms, although numerous genes have been implicated in pathogenesis. (cdc.gov)

Whereas host genetic susceptibility plays a key role in modulating disease manifestation, variations in bacterial virulence properties contribute to infection severity. (cdc.gov)
Adhesion of F. columnare to gill tissue is correlated to virulence and host susceptibility. (auburn.edu)
As the fasting impacts susceptibility to F.columnare, in gill and skin 7 day fasting significantly altered expression of critical innate immune factors. (auburn.edu)
Our research has demonstrated that the correlation of bacterial pigments on their susceptibility to antimicrobial agents. (biomedcentral.com)

Epigenetic analysis revealed a specific N 6 -methyl adenine (m 6 A) methylating pattern including methylation of alginate, flagellar and quorum sensing associated genes. (frontiersin.org)
Functional characterization of the identified regions found relationships between methylation at these loci and nearby genetic variation contributing to chronic painful TMD and with gene expression of proximal genes. (iasp-pain.org)
These findings reveal epigenetic contributions to chronic painful TMD through methylation of the genes FMOD, PM20D1, ZNF718, ZFP57, and RNF39, following the development of acute painful TMD. (iasp-pain.org)
Dirk Schübeler has done important work in epigenomics and regulatory genomics, in particular with the dynamics of genome-wide methylation patterns, their role in gene regulation, and the interplay between chromatin state and the actions of transcription factors. (unibas.ch)

Download Figure?S3, TIF file, 0.1 MB mbo001131461sf03.tif (151K) GUID:?AEC0786A-9B1D-4C73-B74D-4379D40E074F Table?S1: Bacterial strains and plasmids used in this study. (phytid.org)
Bacterial strains and plasmids are described in Table 1 . (hindawi.com)

Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. (bvsalud.org)
At low cell density V. cholerae activates the expression of virulence factors and forms biofilms. (princeton.edu)
The two variants exhibit different capability to form biofilms and to express genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system [ 18 ]. (hindawi.com)

We validated the algorithm's predictions by characterizing 646 genetic system variants, encoded in plasmids and genomes, expressed in six gram-positive and gram-negative bacterial hosts. (omictools.com)
A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae , and also allowing us to assign potential functions to previously uncharacterized TFs. (biomedcentral.com)
We have evaluated the orthologous distribution of these elements in other sequenced bacterial genomes using the repertoire of TFs identified in USA300, and identified a core set of regulators for both the Firmicutes phylum, and the Staphylococacceae group. (biomedcentral.com)
However, this analysis requires accurate reference genomes to identify the specific genes from which RNA reads originate. (osti.gov)
Sample-specific meta-genomic assembled genomes (MAGs) were used as reference genomes to accurately identify the origin of RNA reads, and transcript ratios of genes with opposite transcription responses were compared to eliminate biases related to differences in organismal abundance, an approach hereafter named the "diametric ratio" method. (osti.gov)
Homology-based reconstruction of regulatory networks for bacterial and archaeal genomes. (openwetware.org)

Plasmids have been widely used in biotechnology and genetic engineering, as they can be manipulated to introduce, modify, or delete specific genes in the host cell. (stemcelldaily.com)
Plasmids can also be used to study gene expression and regulation, gene function and interaction, and gene therapy. (stemcelldaily.com)
Plasmids often carry genes that confer some advantage to the host cell, such as antibiotic resistance, virulence factors, or metabolic capabilities. (stemcelldaily.com)
Plasmids are also known as sex factors , conjugants , extra chromosomal replicons , or transfer factors . (stemcelldaily.com)
Compatibility of plasmids - this refers to the ability of two different plasmids to coexist in the same bacterial cell. (stemcelldaily.com)
Some plasmids are compatible with each other and can be maintained together in a stable state, while others are incompatible and compete for resources or interfere with each other`s replication or expression. (stemcelldaily.com)

It is quite resistant to antimicrobials and has several virulence factors that contribute to its high pathogenicity. (usp.br)

The expression of retS over time was also evaluated by western blot when co-cultivated with Staphylococcus aureus and Candida albicans in artificial sputum media and we noticed that there was no change in the expression of this gene when compared to the axenically cultured PAO1. (usp.br)
S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors (TFs) are the primary elements. (biomedcentral.com)
In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757. (biomedcentral.com)
Hospital associated (HA) and community associated (CA) infections caused by methicillin-resistant S. aureus (MRSA) have become a major public health concern, particularly for CA-MRSA infections as they cause life threatening disease in otherwise healthy individuals with no pre-existing risk factors [ 1 ]. (biomedcentral.com)
As virulence determinant production is very tightly regulated in S. aureus , a thorough understanding of its regulatory network is necessary to fully comprehend the pathogenic processes of this bacterium. (biomedcentral.com)
Even though diverse strains of S. aureus have been extensively studied, and subjected to genome sequencing, the function of a large proportion of their genes remains unidentified. (biomedcentral.com)
Finally, we examine the conservation of 135 USA300 TFs amongst 11 other S. aureus strains, identifying a key group of regulators that display a high degree of conservation, including many that have previously been demonstrated to play a role in virulence gene regulation. (biomedcentral.com)
2023. The gene regulatory network of Staphylococcus aureus ST239-SCCmecIII strain Bmb9393 and assessment of genes associated with the biofilm in diverse backgrounds. (openwetware.org)

Among several EPS-defective mutants, we identified a mutant, SL341P4, with a Tn5-insertion in a gene encoding a putative NDP-sugar epimerase, a putative membrane protein with sugar-modifying moiety, in a reverse orientation to EPS biosynthesis gene cluster. (ppjonline.org)
We then combined the search algorithm with system-level kinetic modeling, requiring the construction and characterization of 73 variants to build a sequence-expression-activity map (SEAMAP) for a biosynthesis pathway. (omictools.com)
These alternative sigma factors are further classified by the physiological processes that they control, for example, stress response and flagella biosynthesis [ 3 , 4 ]. (hindawi.com)

Synthetic (S)-3-hydroxytridecan-4-one functions as effectively as natural CAI-1 in repressing production of the canonical virulence factor TCP (toxin co-regulated pilus). (princeton.edu)
Furthermore, the C. difficile toxin genes (tcdA and tcdB) and their regulators (tcdR and tcdC) are direct CcpA targets. (studyres.com)
The spectrum of diseases caused by C. difﬁcile is highly variable and depends not only on host factors but also, for the severe forms, on the level of toxin production (4). (studyres.com)
This is a gene that confers resistance to a certain antibiotic or toxin, which helps in identifying and selecting the cells that contain the plasmid. (stemcelldaily.com)

Most of this regulation is carried out directly by transcription factors that modulate the activity of promoters aimed at expressing virulence factors. (usp.br)
Using gene expression profiling, in silico analysis, and reverse genetic approaches, we have linked to the control of recovery from infection to the function of conserved transcription factors, including th GATA transcription factor ELT-2, the FOXO transcription factor DAF-16, and the Nrf transcription factor SKN-1. (grantome.com)
Given the conserved nature of GPCR-mediated signaling and the conservation of the transcription factors involved in the control of recovery, the proposed studies should lead to a better understanding of the mechanisms used by metazoans to control recovery from bacterial infection at the whole animal level. (grantome.com)
In this context, DNA-binding transcription factors (TFs) play an important regulatory role by either repressing or activating genes in response to environmental and physiological conditions. (biomedcentral.com)
2023. Editorial: Role of Transcription Factors and Sigma Factors in Bacterial Stress. (openwetware.org)
Comparative genomics of DNA-binding transcription factors in archaeal and bacterial organisms. (openwetware.org)

These findings suggest that CAI-1 could be used as a therapy to prevent cholera infection and, furthermore, that strategies to manipulate bacterial quorum sensing hold promise in the clinical arena. (princeton.edu)
In order to characterize the immune actors and events taking place at the mucosal surfaces and identify the routes of pathogen attachment and entry, RNA-seq and microarray approaches were utilized to broadly investaged the transcriptional effects during bacterial infection in channel catfish. (auburn.edu)
At critical early timepoints following challenge in the skin, microarray analyses found that A. hydrophila infection rapidly altered a number of mucosal factors in a manner predicted to enhance its ability to adhere to and invade the catfish host. (auburn.edu)
Deletion of either gene (GBAA0552 or GBAA1340) resulted in attenuation in a murine model of infection. (omictools.com)
To recover from an infection and return to homeostasis, the host must activate mechanisms capable of controlling the damage caused by pathogen virulence factors, inflammation, and a potentially toxic antibiotic exposure. (grantome.com)
Our results show the key role of immune subversion in effective human infection by small bacterial populations. (pasteur.fr)
We speculate that cagY-dependent modulation of H. pylori T4SS function is mediated by alterations in binding to a5ß1 integrin, which in turn regulates the host inflammatory response so as to maximize persistent infection.IMPORTANCE Infection with H. pylori can cause peptic ulcers and is the most important risk factor for gastric cancer, the third most common cause of cancer death worldwide. (pacb.com)
The major H. pylori virulence factor that determines whether infection causes disease or asymptomatic colonization is the type IV secretion system (T4SS), a sort of molecular syringe that injects bacterial products into gastric epithelial cells and alters host cell physiology. (pacb.com)

Elucidating the mechanisms controlling gene expression is important to the understanding of human health. (pressbooks.pub)
Filipowicz, W., Bhattacharyya, S. N. & Sonenberg, N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? (nature.com)
Meister, G. & Tuschl, T. Mechanisms of gene silencing by double-stranded RNA. (nature.com)
This proposal describes experiments designed to understand the mechanisms involved in the control of recovery from bacterial infections. (grantome.com)
These mechanisms are very diverse in the detail, but share commonalities whose quantification should enlighten the evolution of virulence from both a molecular and an ecological perspective. (pasteur.fr)
Blencowe's research team studies the mechanisms by which genes are regulated and coordinated to provide critical functions in mammalian cells. (unibas.ch)

This organism produces a diverse array of virulence factors, including toxins, adhesins, colonization and biofilm factors. (biomedcentral.com)

Regulation of bacterial quorum sensing signaling systems to inhibit pathogenesis represents a new approach to antimicrobial therapy in the treatment of infectious diseases. (absin-reagent.com)

The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
They are extrachromosomal and not essential for the survival of the cell, but they may confer some advantages such as antibiotic resistance or virulence. (stemcelldaily.com)

fis is an E. coli gene encoding the Fis (or FIS) protein. (wikipedia.org)
The regulation of this gene is more complex than most other genes in the E. coli genome, as Fis is an important protein which regulates expression of other genes. (wikipedia.org)
Fis is a very important small nucleotide-associated protein which plays a role in affecting the bacterial chromosome structure and the initiation of DNA replication. (wikipedia.org)
fis mRNA levels closely resemble the protein expression pattern, suggesting that regulation occurs largely at the transcriptional level. (wikipedia.org)
Fis has been deemed a bacterial chromatin architectural protein. (wikipedia.org)
The key interests lie within the field of genome integrity and variation, cell division, regulation of gene expression, protein turnover and signal transduction. (ki.se)
Download Figure?S1, TIF file, 0.1 MB mbo001131461sf01.tif (122K) GUID:?19339782-EB36-4CB4-92F6-9CB926BBA044 Figure?S2: ExoU protein in supernatants of ATC-induced cultures is secreted and not due to bacterial lysis. (phytid.org)
Creating sequence-expression-activity maps accelerates the optimization of many protein systems and allows previous measurements to quantitatively inform future designs. (omictools.com)
Sjöberg A., Trouw L., McGrath F., Hack E. C. and Blom A. M. (2006) Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein. (lu.se)
In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)

Genomic DNA contains both structural gene s, which encode products that serve as cellular structures or enzymes, and regulatory gene s, which encode products that regulate gene expression. (pressbooks.pub)
Genomic characterization predicted specific genomic islands highly associated with virulence, transcriptional regulation, and DNA restriction-modification systems. (frontiersin.org)

Ralstonia solanacearum species complex (RSSC) is a soil borne plant pathogen causing bacterial wilt on various important crops, including Solanaceae plants. (ppjonline.org)

These data define the transcriptional changes induced during growth in low iron conditions and illustrate the potential of this dataset in the identification of putative virulence determinants for future study. (omictools.com)

To search for sRNAs in A. pleuropneumoniae, we developed a strategy for the computational analysis of the bacterial genome by using four algorithms with different approaches, followed by experimental validation. (bvsalud.org)
The whole genome of various RSSC strains have been analyzed, and its virulence mechanism has been extensively investigated. (ppjonline.org)
Using combination of chromatin immunoprecipitation and genome sequence analysis, we detected 55 CcpA binding sites corresponding to 140 genes directly controlled by CcpA. (studyres.com)
Summary: Comparative genomics remains a pivotal strategy to study the evolution of gene organization, and this primacy is reinforced by the growing number of full genome sequences available in public repositories. (omictools.com)
Genome-wide transcript analysis showed that B. anthracis undergoes considerable changes in gene expression during growth in iron-depleted media, including the regulation of known and candidate virulence factors. (omictools.com)
Infectious dose is not associated with genome size and shows insignificant phylogenetic inertia, in line with frequent virulence shifts associated with the horizontal gene transfer of a small number of virulence factors. (pasteur.fr)
Recently, genome sequences revealed that in addition to rpoS , an rpoS -like sigma factor was found in V. alginolyticus 12G01. (hindawi.com)

Using QS as a model will allow us to understand long-standing questions in the field related to signal recognition preferences in single species and multi-species environments and the regulation of the interrelated signaling cascade that exists to coordinate behavior. (wadsworth.org)
tracrRNA-mediated crRNA maturation is conserved among different bacterial species. (nature.com)
Structure of Co-expression Networks of Bifidobacterium species in Response to Human Milk Oligosaccharides. (openwetware.org)

The RNA chaperone Hfq promotes the association of small RNAs (sRNAs) with cognate mRNAs, controlling the expression of bacterial phenotype. (bvsalud.org)
This MHC phenotype is dominantly enhanced by removal of rok or of other members of the PCP effector gene family. (uni-wuerzburg.de)

In addition, the GtrS/GltR pair regulates the expression of toxA that encodes exotoxin A, the primary virulence factor. (nih.gov)

For this, the upstream regions of the gacA, gacS, ladS, retS, rsmA, rsmZ and PA1611 genes were cloned separately into a miniTn7 vector and inserted into the PAO1 chromosome. (usp.br)
They are not part of the main chromosome of the cell, but they can replicate independently and carry genes that may confer some advantages to the host organism. (stemcelldaily.com)

Despite a large number of studies exploring the regulation of type III secretion gene, which encodes the highly cytotoxic type III effector ExoU, is induced early during acute pneumonia. (phytid.org)
The MCS is usually located within a lacZ gene, which encodes for β-galactosidase enzyme. (stemcelldaily.com)

The physiological conditions experienced by a microbial community can thus be inferred using meta-transcriptomic sequencing by comparing transcription levels of specifically chosen genes. (osti.gov)

We have found that the amino acid D-serine drives niche adaptation and bacterial evolution by affecting gene selection in E. coli. (ukri.org)

Essentially nothing is known about the signal transduction pathways involved in the observed differential expression of virulence genes. (ed.ac.uk)
Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)
Moreover, regulation of AtGRP7 downstream targets also depends on its RNA-binding activity, as AtGRP8 and other targets identified by transcript profiling of wild-type and AtGRP7-ox plants using fluorescent differential display are negatively affected by AtGRP7 but not by AtGRP7-RQ. (uni-bielefeld.de)

Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. (bvsalud.org)
To identify genes in EPS production beyond the EPS biosynthetic gene operon, we selected the EPS-defective mutants of R. pseudosolanacearum strain SL341 from Tn5-inserted mutant pool. (ppjonline.org)
The importance of early expression was examined using a strain of with inducible production of ExoU. (phytid.org)
To investigate the response of LuxS01 and wild type strain to exogenously added AI-2, cells from Venetoclax in vitro experiments A and B were harvested in late exponential phase for RNA extraction and microarray gene expression analysis. (hsdpathway.com)

The expression of the bacterial genes involved in the different steps of the infectious process--invasion, intracellular multiplication and spreading--is temporally and spatially controlled, thus ensuring the presence of the respective gene products at the right moment and place. (ed.ac.uk)
CONCLUSION: The results suggest that the MAV_2928 gene/operon might participate in the establishment of bacterial intracellular environment in macrophages. (oregonstate.edu)

however, simultaneous challenges by abiotic and biotic stress factors results in the activation of diverse signals that can have synergistic and antagonistic effects on each other. (usda.gov)
They partially and/or temporarily replace the vegetative sigma factor and are thus crucial for many stress responses [ 2 ]. (hindawi.com)
Our work revealed that A. muciniphila attenuated APAP-induced liver injury by alleviating oxidative stress and inflammation in the liver, and its hepatoprotective effect was accompanied by activation of the PI3K/Akt pathway and mediated by regulation of the composition and metabolic function of the intestinal microbiota. (typeset.io)

Gene regulatory network inference and gene module regulating virulence in Fusarium oxysporum. (openwetware.org)

Understanding the interaction between the gene expression of a pathogen and that of its human host is important for the understanding of a particular infectious disease. (pressbooks.pub)

Expression of this fis operon is negatively regulated by Fis in vivo and purified Fis can prevent stable complex formation by RNA polymerase at the fis promoter in vitro. (wikipedia.org)
For example, in E. coli , all of the structural genes that encode enzymes needed to use lactose as an energy source lie next to each other in the lactose (or lac ) operon under the control of a single promoter, the lac promoter. (pressbooks.pub)

In our study, we hypothesized that both have a role in the activation of the upstream region of the retS in LB media and MOPS glucose since their mutation led to higher expression of the upstream region. (usp.br)
About 18% of all C. difficile genes are regulated by glucose, for which 50% depend on CcpA for regulation. (studyres.com)

A total of 135 TFs and sigma factors were identified and classified into 36 regulatory families. (biomedcentral.com)
Within the RNAP holoenzyme, sigma factors provide promoter recognition specificity to the polymerase and contribute to DNA strand separation, then they dissociate from RNAP core enzyme and transcription initiation follows [ 1 ]. (hindawi.com)
Alternative sigma factors are synthesized or activated in response to certain environmental conditions. (hindawi.com)

Thus, although most regulation of gene expression occurs through transcriptional control in prokaryotes, regulation of gene expression in eukaryotes occurs at the transcriptional level and post-transcriptionally (after the primary transcript has been made). (pressbooks.pub)

Nesse sentido, objetivamos caracterizar o comportamento de diferentes promotores de genes envolvidos na regulação de biofilme de P. aeruginosa sob diferentes condições e buscar possíveis novas proteínas de ligação ao DNA associadas às suas regiões promotoras. (usp.br)
In the recent past, the presence and function of a ROSE-like RNA-thermometer located in the 5′UTR of the rhamnosyltransferase genes rhlAB has been reported in wild type P. aeruginosa . (springer.com)
For this purpose, the non-pathogenic production host P. putida KT2440 containing the rhlAB genes with the native P. aeruginosa 5′-UTR region was used. (springer.com)

accordingly, the fittest members of this diverse bacterial community are selected to survive and invade host tissue. (cdc.gov)
He identified periodic acid-Schiff (PAS)-staining granules, most likely representing degenerating bacterial forms, within macrophages isolated from the small bowel as well as other tissue and fluid specimens (eg, pericardium, endocardium, lymph nodes, synovia, lung, brain, meninges) obtained from patients in whom Whipple disease was suspected. (medscape.com)

Gene regulation involves a complex web of interactions within a given cell among signals from the cell's environment, signalling molecules within the cell, and the cell's DNA. (pressbooks.pub)
These interactions lead to the expression of some genes and the suppression of others, depending on circumstances. (pressbooks.pub)

We found that genes that are markers of innate immunity are downregulated upon recovery, while genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis are upregulated. (grantome.com)
We will identify and characterize trichothecene detoxification genes from microbes capable of surviving mycotoxin exposure. (usda.gov)

These two autoinducers, cholerae autoinducer-1 (CAI-1) and autoinducer-2 (AI-2), function synergistically to control gene regulation, although CAI-1 is the stronger of the two signals. (princeton.edu)
Here we describe the purification of CAI-1 and identify the molecule as (S)-3-hydroxytridecan-4-one, a new type of bacterial autoinducer. (princeton.edu)

Objective 1: Identify and characterize microorganisms and microbial genes that can reduce trichothecene contamination of grain-based food and feed. (usda.gov)

Although this organism possesses many virulence factors, the type III secretion system plays an especially important role in both animal models and humans. (phytid.org)

Current antibiotics work by interfering with bacterial growth, which is a fine game plan… until it stops working. (wadsworth.org)

Our study reveals a novel pathway of small guide RNA maturation and the first example of a host factor (RNase III) required for bacterial RNA-mediated immunity against invaders. (nature.com)
Both tracrRNA and pre-crRNA confer immunity against acquisition of a protospacer gene derived from a lysogenic phage. (nature.com)

Fermentable carbon sources seem to have a particular role in virulence gene regulation. (ed.ac.uk)

Because of its unusual prevalence, global spread, and increased virulence, we investigated the unique features that likely confer its unusual properties. (cdc.gov)

Virulence-related Mycobacterium avium subsp hominissuis MAV_2928 gene is associated with vacuole remodeling in macrophages. (oregonstate.edu)

So far, one network which is involved in the regulation of listerial virulence, the PrfA regulon, has been characterized rather well. (ed.ac.uk)
The analysis of virulence gene expression in vivo, i.e. in infected host cells, indicates that yet uncharacterized bacterial factors other than PrfA, and possibly also host factors, modulate the expression of the PrfA regulon. (ed.ac.uk)

In this way, regulation of the transcription of all of the structural genes encoding the enzymes that catalyze the many steps in a single biochemical pathway can be controlled simultaneously, because they will either all be needed at the same time, or none will be needed. (pressbooks.pub)

Surprisingly, in vivo a similar increase in expression from the above promoters was observed for the mutant deficient in GltR regardless of the presence of effectors. (nih.gov)
This correlates with the loss of the negative auto-regulation in vivo: in transgenic plants constitutively overexpressing AtGRP7 (AtGRP7-ox), a shift occurs to an alternatively spliced AtGRP7 transcript that decays rapidly, and thus does not accumulate to high levels. (uni-bielefeld.de)

We then evaluated the growth and activity of the upstream regions by lux expression under different carbon sources and iron deprivation. (usp.br)

Anatomy20

Organisms37

Diseases15

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes84

Disciplines and Occupations3

Information Science6

Health Care1

Biofilm formation5

Proteins9

Bacteria17

Pseudomonas4

Major virulence factors1

Metabolism7

Pathogens4

Susceptibility4

Methylation4

Strains and plasmids2

Biofilms3

Genomes6

Plasmids6

Pathogenicity1

Aureus8

Biosynthesis3

Toxin4

Transcription factors6

Infection8

Mechanisms6

Toxins1

Pathogenesis1

Antibiotic2

Protein10

Genomic2

Plant pathogen1

Determinants1

Genome7

Species3

Phenotype2

Regulates the expression1

Chromosome2

Encodes2

Physiological1

Adaptation1

Differential3

Strain4

Intracellular2

Stress3

Regulatory network1

Infectious1

Operon2

Glucose2

Sigma factors3

Transcriptional level1

Aeruginosa3

Tissue2

Interactions2

Detoxification2

Autoinducer2

Characterize1

Organism1

Antibiotics1

Immunity2

Role1

Confer1

Macrophages1

Regulon2

Enzymes1

Vivo2

Carbon sources1