Viral proteins protein sequence and structural sequence identity protein residues. Medical search. Frequent questions

Finally, whole-genome sequencing revealed early detection of the B1 clade in 2009 and continued evolution of the B3 clade from 2016 to 2018. (cdc.gov)
The viral genome is located inside the capsid of the virion, having a positive-sense polarity and a long open reading frame, which is flanked by untranslated regions at the 5' and 3' ends. (spandidos-publications.com)
To better define the structural basis of genome packaging and organization in herpes simplex virus type 1 (HSV-1), we developed sequential localized classification and symmetry relaxation methods to process cryo-electron microscopy (cryo-EM) images of HSV-1 virions, which enabled us to decouple and reconstruct hetero-symmetric and asymmetric elements within the pseudo-icosahedral capsid. (rcsb.org)
Finally, our atomic models of portal vertex elements reveal how the fivefold-related capsid accommodates symmetry mismatch imparted by the dodecameric portal-a longstanding mystery in icosahedral viruses-and inform possible DNA-sequence recognition and headful-sensing pathways involved in genome packaging. (rcsb.org)
The RNA genome of SARS-CoV-2 is one of the largest RNA genomes among RNA viruses [1], comprised of a replicase gene which encodes non-structural proteins (nsps), structural proteins, and accessory proteins. (insidecorona.net)
The viral genome encodes four structural capsid proteins (VP1 to VP4) and seven nonstructural (NS) proteins, the leader Lb/ab protease, and proteins encoded in the P2 (2B and 2C) and P3 (3A, 3B, 3C, and 3D) regions ( 9 ). (asm.org)
As this step is critical for the viral genome replication, it makes RT an attractive target for antiviral treatment. (biomedcentral.com)
Here, we reported the first Oceanospirillum phage, vB_OliS_GJ44, which was assembled into a 33,786 bp linear dsDNA genome, which includes abundant tail-related and recombinant proteins. (biomedcentral.com)
During infection, the viral genome is translated to generate viral polyproteins and transcribed to generate negative-sense RNA and subgenomic RNAs. (xiahepublishing.com)
The SARS-CoV-2 genome contains 14 open reading frames (ORFs) that encode 29 proteins, including nonstructural proteins (NSPs), structural proteins, and accessory proteins. (xiahepublishing.com)
51) provided detailed annotations of the then- recently sequenced SARS-CoV genome (35, 47), including the identification of a domain unique to SARS-CoV and the pre- diction of the ADP-ribose-1�-phosphatase (ADRP) activity of the X domain (since shown to be one of the macrodomains). (pdfroom.com)
There are said to be sequences of HIV-1 in the SARS-CoV-2 genome. (changingtimes.media)
Yan and others say there is evidence within the spike protein of the SARS-CoV-2 genome that suggests it is a product of genetic manipulation. (changingtimes.media)
On January 12, 2020, Chinese scientists disclosed and reported the SARS-CoV-2 genome sequence, which was then uploaded to the GenBank database [ 6 ]. (springeropen.com)
As the total genome sequencing of the Corona virus is done, the complexity has been studied by scientists. (springeropen.com)

Once translated, these polyproteins are cleaved by one of the two encoded proteases (3C-like protease (nsp5) or papain-like-protease (nsp3)) to yield between 15 and 16 non-structural proteins, which assemble into a large membrane-bound replicase complex (RTC). (insidecorona.net)
The viral particle is composed of a protein capsid that contains a positive-sense RNA molecule of about 8,500 nucleotides that is infectious and encodes a single polyprotein, which is processed in infected cells by cis - and trans -acting viral proteases ( 55 ) to yield different polypeptide precursors and the mature viral proteins ( 9 , 62 ). (asm.org)
The two main units, ORF1a and ORF1b, are located at the 5′-terminus and produce 16 NSPs through proteolytic cleavage by two viral proteases: the 3C-like protease and the papain-like protease. (xiahepublishing.com)
Receptor binding activates proteases that can carry out proteolytic cleavage of the S protein. (pubannotation.org)
The main issue with gluten proteins is that the human digestive proteases can only partially degrade them leading to a mixture of peptides that elicit immune and toxic effects in predisposed individuals and cell lines [ 8 ] . (encyclopedia.pub)
These proteins inhibit the C3 and C5 convertases (multi-subunit proteases), by promoting dissociation of the multisubunit complexes and/or by inactivating the complexes through proteolysis (catalyzed by factor I). Several pharmacological agents that regulate or modulate complement activity have been identified by in vitro assay, but most have been shown in vivo to be of low activity or toxic. (justia.com)
Among coronaviruses, the main protease (Mpro) is an essential drug target which, along with papain-like proteases catalyzes the processing of polyproteins translated from viral RNA and recognizes specific cleavage sites. (bvsalud.org)

The Spike proteins decorate the exterior of the coronavirus particles. (wearechange.org)
To develop specific anti-coronavirus therapeutics and prophylactics, the molecular mechanism that underlies viral infection must first be defined. (nature.com)
The goal of the present investigation is to examine the effects of missense mutations in the MERS-CoV S protein on protein stability and binding affinity with DPP4 to provide insight that is useful in developing vaccines to prevent coronavirus infection. (frontiersin.org)
These findings provide further information on how mutations in coronavirus S proteins effect protein function. (frontiersin.org)
Infection of human cells by the novel coronavirus (SARS-Cov-2) involves the attachment of the receptor binding domain (RBD) of the spike protein to the peripheral membrane ACE2 receptors. (biorxiv.org)
Mutation on these residues can lead to new strains of coronavirus with different degrees of infectivity and virulence. (biorxiv.org)
These residues can not only be targeted by broad spectrum antibodies and drugs, mutations in them can generate new strains of coronavirus resulting in future epidemic. (biorxiv.org)
The N-terminal domain of the nucleocapsid protein from Middle East respiratory syndrome coronavirus (MERS-CoV NP-NTD) contains many positively charged residues and has been identified to be responsible for RNA binding during ribonucleocapsid formation by the virus. (iucr.org)
The coronavirus replication cycle begins with the translation of the 29-kb positive-strand genomic RNA to produce two large polyprotein species (pp1a and pp1ab), which are subse- quently cleaved to produce 15 or possibly 16 nonstructural proteins (nsp's) (11). (pdfroom.com)
Among these, nsp3 is the largest nsp and also the largest coronavirus protein. (pdfroom.com)
An extensive bioinformatics analysis of the coronavirus replicase proteins by Snijder et al. (pdfroom.com)

Similar to what is observed for other viral proteins, S2 of SARS-CoV-2 shares a high sequence identity (95%) with S2 of ZC45/ZXC21. (wearechange.org)
Clearly, despite the high sequence identity for the overall genomes, the RBM of SARS-CoV-2 differs significantly from those of ZC45 and ZXC21. (wearechange.org)
Judging from this sequence analysis alone, we were convinced early on that not only would the SARS-CoV-2 Spike protein bind hACE2 but also the binding would resemble, precisely, that between the original SARS Spike protein and hACE223. (wearechange.org)
Therefore, we herein established a SARS-CoV-2 spike (S) protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV. (nature.com)
We solved the X-ray crystal structure of six-helical bundle (6-HB) core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit, revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain. (nature.com)
Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM, about 241- and 149-fold more potent than the original EK1 peptide, respectively. (nature.com)
Fig. 1: Establishment of SARS-CoV-2 S protein-mediated cell-cell fusion system. (nature.com)
a The emerging timeline for highly pathogenic viruses and the proposed Disease X. b Schematic representation of SARS-CoV-2 S protein. (nature.com)
We compared the S protein structures of MERS-CoV, SARS-CoV, and SARS-CoV-2 viruses and identified the residues like C526, C383, and N468 located in equivalent positions of these viruses have effects on S protein structure. (frontiersin.org)
The etiological agent, SARS-CoV-2, is a member of the Coronaviridae family including SARS-CoV-1 (2002-2004) and MERS-CoV (since 2012), with the sequence identity of 79.6% and 50%, respectively. (biorxiv.org)
1 , 2 Largely expressed on the surface of SARS-CoV-2, the spike (S) protein plays a crucial role in binding to the host angiotensin-converting enzyme 2 (ACE2) through the receptor-binding domain (RBD) and facilitating viral entry, 3 , 4 which is therefore considered as one of the most preferred targets against SARS-CoV-2. (biorxiv.org)
5 , 7 - 9 More specifically, a study analyzing 10,022 SARS-CoV-2 genomes from 68 countries revealed 2969 different missense variants, with 427 variants in the S protein. (biorxiv.org)
This review summarises the structural and functional information gained from SARS-CoV-2, SARs-CoV and MERS-CoV nsp15 structures, compiles the current structure-based drug design efforts, and complementary knowledge with a view to provide a clear starting point for downstream structure users interested in studying nsp15 as a novel drug target to treat COVID-19. (insidecorona.net)
Although highly conserved (88% sequence identity with SARS-CoV-2, 50% with MERS, and 43% with HCoV-229E), nsp15 has been found to be non-essential for viral replication in Mouse Hepatitis Virus [6] (MHV), SARS-CoV, and HCoV-229E. (insidecorona.net)
Nonstructural protein 15 (NSP15) is a promising target due to its importance for SARS-CoV-2's evasion of the host's innate immune response. (xiahepublishing.com)
Targeting viral proteins to disrupt replication is an important approach to develop a therapy against SARS-CoV-2 infection. (xiahepublishing.com)
Recent studies have reported SARS-CoV-2 genomic variations in over 10% of isolated sequences, with the most frequent mutations being P323L in NSP12 and D641G in the spike protein. (xiahepublishing.com)
After the introduction of mutation to the residue insertion and furin cleavage site, the S1/S2 cleavage of the SARS-CoV 2 S did not longer take place. (pubannotation.org)
Researchers say there's evidence within the SARS-CoV-2 spike protein that suggests the virus is a product of genetic manipulation. (changingtimes.media)
The knowledge of SARS-CoV-2 contagion and latency is currently poor, raising concerns about viral persistence. (springeropen.com)
However, SARS-CoV-2 mutations resulting in the emergence of new viral strains that can be resistant to the drugs used currently in the clinic necessitate the development of novel potent and broad therapeutic agents targeting different vulnerable spots of the viral proteins. (bvsalud.org)
In this study, two deep learning generative models were developed and used in combination with molecular modeling tools for de novo design of small molecule compounds that can inhibit the catalytic activity of SARS-CoV-2 main protease (Mpro), an enzyme critically important for mediating viral replication and transcription. (bvsalud.org)
Analysis of the SARS-CoV-2 sequence revealed a multibasic furin cleavage site at the S1/S2 boundary of the spike protein distinguishing this virus from SARS-CoV. (bvsalud.org)
Cleavage of SARS-CoV-2 spike protein by furin promotes viral entry into lung cells. (bvsalud.org)
While furin knockout is embryonically lethal, its knockout in differentiated somatic cells is not, thus furin provides an exciting therapeutic target for viral pathogens including SARS-CoV-2 and bacterial infections. (bvsalud.org)
Therefore, targeting the SARS-CoV-2 Mpro enzyme with small molecules can block viral replication. (bvsalud.org)
The SARS-CoV-2 Mpro shows identities of 96.08% and 50.65% to that of SARS-CoV Mpro and MERS-CoV Mpro respectively at the sequence level. (bvsalud.org)
At the structural level, the root mean square deviation (RMSD) between SARS-CoV-2 Mpro and SARS-CoV Mpro was found to be 0.517 Å and 0.817 Å between SARS-CoV-2 Mpro and MERS-CoV Mpro. (bvsalud.org)

3. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease, this segment is a peptide able to bind to NS3pro and contact at least one residue comprised in the region Gly21-Lys28 of the N-terminal domain of the NS3pro protein from DV2 or structurally equivalent residues of the NS3pro protein from other Flavivirus. (patentsencyclopedia.com)
11. Chimerical peptides according to the claim 1, characterized by containing a cell penetrating segment which is one of the following cationic PTDs: TAT peptide, heptaarginine, octaarginine, nonaarginine, decaarginine, or a peptide of sequence LRRRRLRRRRL or LRRRRL-bAla-LRRRRL. (patentsencyclopedia.com)
The derived amino acid sequence of Ov-phy-1 encoded a peptide that was most similar to the two Caenorhabditis elegans prolyl 4-hydroxylase homologues and to the isoform II enzymes of vertebrates. (embl.de)
In coronaviruses, cleavage occurs at two sites: the S1/S2 junction and at the S2′, a region close to the viral fusion peptide [2]. (pubannotation.org)
The immunodominant 33-mer peptide comprises residues 57 to 89 of α-2-gliadin (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF). (encyclopedia.pub)
In total, 39% of the 33-mer peptide residues are prolines leading to a type-II polyproline (PPII) conformation in solution, which is known to be bound to MHC class-II molecules [ 14 ] . (encyclopedia.pub)
describes the use of a phage-displayed combinatorial random peptide library to identify a 27-residue peptide that binds to C3 and inhibits complement activation. (justia.com)
This peptide was truncated to a 13-residue cyclic segment that maintained complete activity, which is referred to in the art as Compstatin. (justia.com)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. (lookformedical.com)

It is noncovalently bound to the neck of the viral capsid. (wikipedia.org)
The translated polyprotein consists of three structural [capsid (C), membrane (M) and envelope (E)] and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5). (spandidos-publications.com)
Capsid virus-like particles (cVLPs), assembled from viral coat proteins, are used as therapeutic cargo delivery vehicles as well as molecular scaffolds for display of vaccine antigens. (bvsalud.org)

Replacements L38E and L41E, involving charge acquisition at residues predicted to contribute to the hydrophobic interface, reduced the dimerization signal in the protein ligation assay and prevented the detection of dimer/multimer species in both transiently expressed 3A proteins and in synthetic peptides reproducing the N terminus of 3A. (asm.org)
The present invention is relative to chimerical peptides, whose primary structure holds at least one segment which inhibits the activation of the NS3 protease of a virus from the Flaviviridae family, they also contain a cell penetrating segment and they are capable of inhibiting or attenuate the viral infection. (patentsencyclopedia.com)
5. Chimerical peptides according to the claim 1, where the mentioned segment inhibitor of the activation of the NS3pro protease contact at least one residue comprised in the region Glu32-Thr38 of the N-terminal domain of the NS3pro protein from HCV or structurally equivalent residues of the NS3pro protein from other Hepacivirus. (patentsencyclopedia.com)
6. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Asp50-Glu62 of the NS2B protein from DV2 or the structurally equivalent segment of the protein NS2B from other Flavivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
8. Chimerical peptides according to the claim 1, characterized by containing a segment inhibitor of the activation of the NS3pro protease which comprise the region Thr20-Leu31 of the NS4A protein from HCV or the structurally equivalent segment of the protein NS4A from other Hepacivirus, or an amino acid sequence showing a sequence similarity higher of equal to the 80% identity. (patentsencyclopedia.com)
The PPS1 protein was expressed in Escherichia coli, and the protein was shown to catalyze the hydrolysis of p-nitrophenyl phosphate, dephosphorylate phosphotyrosyl, and phosphothreonyl residues in synthetic diphosphorylated peptides and to inactivate the human ERK1 protein. (embl.de)
Bacteriophages offer several advantages as drug carriers, such as high specificity for targeting bacterial cells, low toxicity, and the ability to be engineered to express specific proteins or peptides for enhanced targeting and drug delivery. (bvsalud.org)
Compounds comprising peptides and peptidomimetics capable of binding the C3 protein and inhibiting complement activation are disclosed. (justia.com)
In particular, this invention provides peptides and peptidomimetics capable of binding the C3 protein and inhibiting complement activation. (justia.com)

The loops are characterized with functionally important negatively charged, positively charged, and hydrophobic residues, respectively, together forming a unique "three-fingered molecular claw. (uthscsa.edu)
Chemical shift changes upon the addition of single-stranded RNAs (ssRNAs) identified a group of residues that form a positively charged patch on the protein surface as the binding site responsible for the previously reported affinity for nucleic acids. (pdfroom.com)

Therefore, the present study proposes the three‑dimensional structure of the helicase/protease enzyme of SPONV through homology modeling, using the crystal structure of the Dengue virus‑4 helicase/protease of the same viral family as a template. (spandidos-publications.com)
This drug belongs to the category of HIV protease inhibitors, an enzyme essential for the final cut of the various viral components. (gospanews.net)

P22TSP is a homotrimeric structural protein consisting of 666 amino acids. (wikipedia.org)
This protein is 1,353 amino acids in length, and the monomers assemble to form a homotrimer. (frontiersin.org)
These replacements also led to production of infective viruses that replaced the acidic residues introduced (E) by nonpolar amino acids, indicating that preservation of the hydrophobic interface is essential for virus replication. (asm.org)

Replication of picornaviruses occurs associated to cell endomembranes that are recruited during viral infection ( 25 ). (asm.org)

Nevertheless, while Q44R led to recovery of viruses that maintained the mutation, Q44D resulted in selection of infective viruses with substitution D44E with acidic charge but with structural features similar to those of the parental virus, suggesting that Q44 is involved in functions other than 3A dimerization. (asm.org)
Mutation of the catalytic cysteinyl residue in the active phosphatase domain reduced the in vitro activity of the mutant protein to less than 0.5% of wild type activity, while mutation of the corresponding cysteinyl residue of the inactive phosphatase-like domain had no effect on in vitro activity. (embl.de)

The deduced amino acid sequence of PPS1p shows similarity with protein-tyrosine phosphatases (PTPases) and is most closely related to a subfamily of PTPases that are capable of dephosphorylating phosphoseryl and phosphothreonyl residues as well as phosphotyrosyl residues. (embl.de)
The finding of 4 unique inserts in the 2019-nCoV, all of which have identity /similarity to amino acid residues in key structural proteins of HIV-1 is unlikely to be fortuitous in nature. (gospanews.net)

That is an area, all encoded inside the solitary large exon 13, that in human being encodes 16 repeats of 120 proteins approximately. (angiogenesis-blog.com)

Ideally, one can target highly conserved viral proteins that are unlikely to acquire the resistance of new viral mutants as the outbreak progresses. (xiahepublishing.com)

Furthermore, vitamin C showed hydrophobic interactions with both proteins, two bonds with Arg119, Glu120 of HA, and one bond with Arg74 of NA. (ijpsonline.com)
The molecular binding was stabilized through four hydrogen bonds with Glu166 and Thr190 as well as hydrophobic interactions via eight residues. (bvsalud.org)

In this way, AFM allows obtaining nanometric resolution images of individual protein shells, such as viruses, in liquid milieu. (bvsalud.org)
RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
Here, we performed a time-resolved phylogenetic analysis of 129 HA sequences representing all 1891 available H5N8 viruses collected from 2010 to 2020. (mdpi.com)
Sanmenxia Clade 2.3.2.1c-like H5N1 viruses possess the closest genetic identity to A/Alberta/01/2014 (H5N1), which recently caused a fatal respiratory infection in Canada with signs of meningoencephalitis, a highly unusual symptom with influenza infections in humans. (nature.com)

Here, using atomistic molecular dynamics simulation, we study the correlations between the RBD dynamics with physically distant residues in the spike protein, and provide a deeper understanding of their role in the infection, including the prediction of important mutations and of distant allosteric binding sites for therapeutics. (biorxiv.org)
We applied time-independent component analysis (tICA) and protein connectivity network model, on all-atom molecular dynamics trajectories, to identify key non-RBD residues, playing crucial role in the conformational transition facilitating spike-receptor binding and infection of human cell. (biorxiv.org)
Large scale screening of therapeutic molecules and antibodies are underway aiming to target the spike protein and consequently prevent infection. (biorxiv.org)
Vaccination is the most cost-effective and efficient method of preventing viral infection. (springeropen.com)

The initial interest in tailspike proteins was in the study of the effect the mutations on protein folding. (wikipedia.org)
We investigated the effects of MERS-CoV S protein viral mutations on protein stability and binding affinity. (frontiersin.org)

The Spike protein is split into two halves (Figure 3). (wearechange.org)
The C-terminal half of the Spike protein is named S2. (wearechange.org)
Conversely, broad spectrum therapeutics like drugs and monoclonal antibodies can be generated targeting these key distant regions of the spike protein. (biorxiv.org)
Significant research effort for drug design against COVID-19 is focused on the receptor binding domain of the spike protein, although this region is prone to mutations causing resistance against therapeutics. (biorxiv.org)

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)

It is anchored within the viral coat and recognizes the O-antigen portion of the lipopolysaccharide (LPS) on the outer-membrane of Gram-negative bacteria. (wikipedia.org)
A key component in the virulence of MERS-CoV is the Spike (S) protein, which binds with the host membrane receptor dipeptidyl peptidase 4 (DPP4). (frontiersin.org)
in poliovirus (PV), the interaction between the RNA replication complex and intracellular membranes appears to be accomplished by proteins 3A and 2C, which have membrane-binding properties ( 11 , 60 ). (asm.org)
The major role of HA involves in the viral entry mechanism and immune recognition through two subunits namely HA1 containing the receptor binding domain and HA2 responsible for the fusion of the virion with the endosomal membrane in the host cell [ 7 ]. (ijpsonline.com)
In vivo, regulation of complement at the C3 and C5 activation steps is provided by both plasma and membrane proteins. (justia.com)
The plasma protein inhibitors are factor H and C4-binding protein, and the regulatory membrane proteins located on cell surfaces are complement receptors 1 (CR1), decay-accelerating factor (DAF), and membrane cofactor protein (MCP). (justia.com)

Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in virus replication, virulence, and host range. (asm.org)

2 SARS-CoV2 cell entry is not complex Fig. 1 is a schematic illustration of the minimum proteins required to mediate the SARS-CoV2 cell entry. (pubannotation.org)

The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
The presence of genes not previously described inpoxviruses, and in some cases in any other known virus, suggestssignificant viral adaptation to the arthropod host and the externalenvironment. (embl-heidelberg.de)
Genes predicting interactions with host cellular mechanismsinclude homologues of the inhibitor of apoptosis protein, stress responseprotein phosphatase 2C, extracellular matrixin metalloproteases,ubiquitin, calcium binding EF-hand protein, glycosyltransferase, and atriacylglyceride lipase. (embl-heidelberg.de)
DNA sequences in genes that interact with regulatory proteins (such as transcription factors) to determine the rate and timing of expression of the genes as well as the beginning and end of the transcript. (nationalacademies.org)

Previous studies have demonstrated that viral RNA helicases represent promising pharmacological targets for antiviral drugs/inhibitors, as they are implicated in viral replication and proliferation. (spandidos-publications.com)

Their viral particles (virions) are small (~50 nm), spherical and enveloped, that incorporate a single-stranded RNA of 9.5-12.5 kb ( 1 ). (spandidos-publications.com)
During the life cycle of HBV, RT utilizes a single-stranded viral genomic RNA as a template to synthesize a hybrid RNA-DNA duplex, and then converts it to double-stranded DNA (ds-DNA). (biomedcentral.com)

Our paper emphasizes the relevance of Extreme Learning Machine (ELM) in Bioinformatics applications by addressing the problem of predicting the disulfide connectivity from protein sequences. (scitevents.org)
Most of these essential residues are precisely preserved, including those involved in disulfide bond formation (C467, C474) and electrostatic interactions (R444, E452, R453, D454), which are pivotal for the structural integrity of the RBM (Figure 3C and 4). (wearechange.org)
Co-expression of Ov-phy-1 with the O. volvulus homologue of protein disulfide isomerase in a baculovirus system resulted in the production of enzymatically active O. volvulus prolyl 4-hydroxylase. (embl.de)

Taken together, our data indicate that diverse mammalian poxviruses use a conserved molecular claw in a C7-like protein to target SAMD9 and overcome host restriction. (uthscsa.edu)
A molecular model of the FMDV 3A protein, derived from the nuclear magnetic resonance (NMR) structure of the poliovirus 3A protein, predicted a hydrophobic interface spanning residues 25 to 44 as the main determinant for 3A dimerization. (asm.org)
Additionally, it could also be possible that unrecognized molecular, structural, and physical parallels between gluten and bacteria have a relevant role. (encyclopedia.pub)

Proteolytic cleavage of the S protein causes conformational changes so that they cannot revert to the original structure and profound enough to prime the S2 subunit for the fusion of viral and cellular membranes. (pubannotation.org)
A family of serine proteinase inhibitors which are similar in amino acid sequence and mechanism of inhibition, but differ in their specificity toward proteolytic enzymes. (lookformedical.com)

It has been suggested that BRO-A and BRO-C are DNA binding proteins that influence host DNA replication and/or transcription ( PUBMED:10888617 ). (embl-heidelberg.de)
We propose thatBRO-A and BRO-C may function as DNA binding proteins that influence hostDNA replication and/or transcription. (embl-heidelberg.de)

They remove the phosphate group from both phospho-tyrosine and phospho-serine/threonine residues. (embl.de)
Any member of the group of ENDOPEPTIDASES containing at the active site a serine residue involved in catalysis. (lookformedical.com)

Both of these tailspike proteins also contain right-handed parallel beta-helices and share similar O-antigen binding and cleavage to P22TSP. (wikipedia.org)

Transgenic animals modified to produce proteins for extraction, purification, and therapeutic use. (nationalacademies.org)

Serum proteins that inhibit, antagonize, or inactivate COMPLEMENT C1 or its subunits. (lookformedical.com)

Taken with the present results, this suggests that the potential use of inhibitors of prolyl 4-hydroxylase to control excessive collagen deposition in pathological fibrosis may be limited by the need to maintain continuous inhibition of collagen hydroxylation so as to facilitate intracellular degradation of the accumulated protein. (embl.de)

In addition, we studied all DPP4 mutations and found the functional substitution R336T weakens both DPP4 protein stability and S-DPP4 binding affinity. (frontiersin.org)
Herein, the predicted atomic structures of five representative sequence variants of the reverse transcriptase protein (RT) of hepatitis B virus (HBV), sampled from patients with rapid or slow response to tenofovir disoproxil fumarate (TDF) treatment, have been examined to identify structural variations between them in order to assess structural and functional properties of HBV-RT variants associated with the differential responses to TDF treatment. (biomedcentral.com)
A lot of the Ki-67 proteins can be predicted to become unstructured and displays hardly any cross-species conservation beyond several conserved functional areas. (angiogenesis-blog.com)

pps1Delta mutant strains did not differ from PPS1 strains under any of the conditions tested, but overexpression of the PPS1 protein in S. cerevisiae led to synchronous growth arrest and to aberrant DNA synthesis. (embl.de)

On the other hand, 3AB presumably anchors 3B in intracellular membranes originated de novo during the early steps of RNA replication, where uridylylated 3B primes the synthesis of nascent viral RNAs ( 2 , 37 , 68 , 69 ). (asm.org)

The MERS-CoV S protein has 21 N-linked glycosylation sites (Wang et al. (frontiersin.org)

Recently, traditional isolation methods, complemented by high-throughput sequencing metagenomics technology, have greatly increased our understanding of the diversity of bacteriophages. (biomedcentral.com)
Combined with the eight environmental viral contigs from metagenomics, this study helps fill the gap in our understanding of the isolation, genomic and evolutionary development of Oceanospirillum bacteriophages and provides new insights into the interactions between hosts and bacteriophages for these important marine hydrocarbon-degrading microbial populations. (biomedcentral.com)

The goal of this paper is to define a technique, based on the geometrical and topological structure of protein surfaces, for the detection and the analysis of sites of possible protein-protein and protein-ligand interactions. (scitevents.org)
Replacements that favored (Q44R) or impaired (Q44D) the polar interactions predicted between residues Q44 and D32 did not abolish dimer formation of transiently expressed 3A, indicating that these interactions are not critical for 3A dimerization. (asm.org)
These results describe the first Oceanospirillum phage, vB_OliS_GJ44, that represents a novel viral cluster and exhibits interesting genetic features related to phage-host interactions and evolution. (biomedcentral.com)
Docking results showed interaction between the proteins individually into selected ligands, except for gelsemine and vitamin E no interactions were shown. (ijpsonline.com)

The S protein is responsible for helping the virus to gain entry to the cell contributing to the overall virulence of these β-CoVs. (frontiersin.org)

Viral and bacterial pathogens have long been suspected of triggering immune responses that are directed toward autoimmunity in CeD. (encyclopedia.pub)

An enzyme that catalyzes the hydrolysis of proteins, including elastin. (lookformedical.com)

Indeed, we found that all in the latter group of proteins bind SAMD9. (uthscsa.edu)
Incredibly, this allows an individual monoclonal antibody to bind nine sites for the proteins. (angiogenesis-blog.com)

Accordingly, protein antigens can be independently expressed and quality-checked prior to conjugation to pre-assembled cVLPs. (bvsalud.org)
Here, we describe considerations for the design of vaccine antigens with genetically fused split-protein (Tag or Catcher) binding partners and provide protocols for the expression and purification of corresponding Tag- or Catcher-AP205 cVLPs from E.coli. (bvsalud.org)
A broad antibody response was detected that included immunoglobulin G (IgG) and IgA subtypes with demonstrable Fc-mediated effector function targeting multiple viral antigens. (cdc.gov)

18 The human immune system started generating antibodies specific to residues outside RBD even at the earlier stage of the pandemic. (biorxiv.org)

When expressed as a recombinant protein in transfected cells, PV 3A cofractionates with endoplasmic reticulum markers ( 66 ), and its single transient expression can disrupt the secretory apparatus ( 23 ) and decrease major histocompatibility complex (MHC) class I expression ( 22 ). (asm.org)

These proteins share 70% sequence identity in their N-terminal domains, but no sequence similarities have been found in the C-terminal domains. (wikipedia.org)

NS proteins are involved in crucial aspects of the viral cycle and pathogenesis, such as rearrangements of intracellular membranes required for endomembrane recruitment and the lysis of host cells ( 1 , 12 , 14 , 18 , 73 ). (asm.org)

The tailspike protein (P22TSP) of Enterobacteria phage P22 mediates the recognition and adhesion between the bacteriophage and the surface of Salmonella enterica cells. (wikipedia.org)

This family includes the N-terminus of baculovirus BRO and ALI motif proteins. (embl-heidelberg.de)
The baculovirus Bro proteins are encoded by a multigene family. (embl-heidelberg.de)

The most crucial NS proteins are the viral helicase and the viral RNA-dependent RNA polymerase (RdRp) ( 2 ). (spandidos-publications.com)

The PPS1 gene of Saccharomyces cerevisiae codes for a dual specificity protein phosphatase with a role in the DNA synthesis phase of the cell cycle. (embl.de)
Analysis of the predicted amino acid sequence suggests that the protein consists of an active phosphatase domain, an inactive phosphatase-like domain, and an NH2-terminal extension. (embl.de)

PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). (lookformedical.com)

They possess several properties that make them attractive as drug delivery vehicles, including their ability to specifically target bacterial cells, their structural diversity, their ease of genetic manipulation, and their biocompatibility. (bvsalud.org)

It is of fundamental importance in determining PROTEIN CONFORMATION. (lookformedical.com)

The Ki-67 do it again area consists of residues phosphorylated by CDK1 during mitosis [3 also,52,53]. (angiogenesis-blog.com)

Two compounds, Compound 1 (1,4-dihydrophenanthrolin-4-one-3-carboxylic acid) and Compound 5 [8-(N-butyl-N-ethylcarbamoyl)-1,4-dihydrophenathrolin-4-one-3-carboxylic acid], with comparable potencies in vivo, were chosen to investigate the effect of the inhibition of the hydroxylation of newly synthesized uterine collagen on the turnover of this protein in vivo. (embl.de)

One of these non-structural proteins is nsp15, a 346 amino acid nidoviral RNA uridylate‐specific and Mn 2+ -dependent [3] endoribonuclease (NendoU). (insidecorona.net)
As a result, the loss of RNA synthesis is thought to be a knock-on effect on neighbouring polyprotein components that are critical for replication, as opposed to a genuine effect on viral replication through lack of nsp15 [6]. (insidecorona.net)
Although not essential for viral replication, recent studies suggest nsp15 plays a role in repressing activation of the host innate immune response [11-13]. (insidecorona.net)

This control is propagated through the alpha-subunit of prolyl 4-hydroxylase where protein levels were previously shown to rise fivefold with increasing cell density. (embl.de)

An alignment from the amino acidity sequence from the human being repeats can be shown in shape?2. (angiogenesis-blog.com)

Anatomy12

Organisms22

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment42

Phenomena and Processes97

Disciplines and Occupations3

Information Science18

Health Care7

Genome15

Proteases7

Coronavirus11

SARS-CoV28

Peptide9

Capsid3

Peptides9

Positively charged2

Protease2

Amino acids3

Recruited during viral in1

Mutation2

Similarity2

Encodes1

Highly conserved1

Hydrophobic interactions2

Viruses4

Infection4

Mutations on protein2

Spike protein4

Specific amino acid1

Membrane6

Nonstructural protein1

Mediate1

Genes4

Antiviral1

Single-stranded2

Disulfide3

Molecular3

Proteolytic2

Transcription2

Serine2

Cleavage1

Therapeutic1

Inhibit1

Inhibitors1

Functional3

Strains1

RNAs1

MERS-CoV1

Bacteriophages2

Interactions4

Virulence1

Pathogens1

Hydrolysis1

Bind2

Antigens3

Antibodies1

Recombinant1

Similarities1

Pathogenesis1

Salmonella1

Baculovirus2

Crucial1

Phosphatase2

Polypeptide1

Genetic1

Conformation1

Consists1

Inhibition1

Nsp153

Subunit1

Alignment1