Viral nucleic acid replication. Medical search. Frequent questions

Although the structures of rotavirus and other members of the Reoviridae have been extensively studied, little is known about the structures of virus-encoded non-structural proteins that are essential for genome replication and packaging. (nih.gov)
Several RNA binding sites, resulting from the quaternary organization of NSP2 monomers, may be required for the helix destabilizing activity of NSP2 and its function during genome replication and packaging. (nih.gov)
The dynamics of genome replication using deep sequencing. (genomeweb.com)
The presence of the 'kissing loop' interaction inhibited the formation of SL9571 in the 3'-X-tail, an RNA structure implicated in genome replication. (ox.ac.uk)

Promising new inhibitors that target the viral helicase-primase complex have been reported to block replication of herpes simplex and varicella-zoster viruses, but they have no activity against human cytomegalovirus (HCMV), another herpesvirus. (frontiersin.org)
Viral replication is the formation of biological viruses during the infection process in the target host cells. (wikipedia.org)
Viruses must first get into the cell before viral replication can occur. (wikipedia.org)
Replication between viruses is greatly varied and depends on the type of genes involved in them. (wikipedia.org)
In animal cells these viruses get into the cell through the process of endocytosis which works through fusing of the virus and fusing of the viral envelope with the cell membrane of the animal cell and in plant cells it enters through the process of pinocytosis which works on pinching of the viruses. (wikipedia.org)
These genomes are made by either a viral polymerase or (in some DNA viruses) a cellular enzyme, particularly in rapidly dividing cells. (wikipedia.org)
Viruses are classed into 7 types of genes, each of which has its own families of viruses, which in turn have differing replication strategies themselves. (wikipedia.org)
David Baltimore, a Nobel Prize-winning biologist, devised a system called the Baltimore Classification System to classify different viruses based on their unique replication strategy. (wikipedia.org)
Some of these viruses require host cell polymerases to replicate their genome, while others, such as adenoviruses or herpes viruses, encode their own replication factors. (wikipedia.org)
Dynein and kinesin can both be exploited by viruses to mediate the viral replication process. (wikipedia.org)
Many viruses use the microtubule transport system to transport nucleic acid/protein cores to intracellular replication sites after invasion host the cell membrane. (wikipedia.org)
2 Viruses enter into a host cell for replication (the process of producing two identical replicas of RNA, ribonucleic acid or DNA, deoxyribonucleic acid) and take over the cell's biological machinery. (icr.org)
Viruses did not evolve from some non-viral entity. (icr.org)
Viruses consist of nucleic acid (viral genome) and a limited number of proteins that facilitate entry into a host cell, replication of the genome and production of virions. (qiagen.com)
Moreover, they infect cells with viruses, or express individual viral proteins, to follow the virus's actions and the host cell response. (the-scientist.com)
Cell cultures have played a critical role in modern infectious disease research, particularly in the area of viruses and the expression of viral gene products," Bloom says. (the-scientist.com)
The team led by Prof. Hiroki Kato from the Institute of Cardiovascular Immunology at the University Hospital Bonn has identified a compound that inhibits the body's own methyltransferase MTr1, thereby limiting the replication of influenza viruses. (uni-bonn.de)
Are required viral genes split between multiple constructs to prevent production of replication-competent viruses? (uwm.edu)
With most DNA viruses, replication takes place in the host's nucleus, but with most RNA viruses, replication takes place in the host cell's cytoplasm. (mometrix.com)
Much of what we know about viral replication has also been learned from studying viruses of bacteria known as phages . (fluther.com)
Viruses in their native state contain only a single type of nucleic acid which may be either DNA or RNA. (brainkart.com)
On the basis of nature of nucleic acid viruses are classified into four Categories. (brainkart.com)
It can also be hijacked by some viruses, such as SARS-CoV-2, to enable their replication. (bellbrooklabs.com)
Like several other DDXs, most notably DDX3, it acts as a viral RNA sensor and contributes to immunity via type I interferon induction, but it is also hijacked by some viruses to enable their propagation. (bellbrooklabs.com)
Some viruses have an outer envelope consisting of protein and lipid, surrounding a protein capsid complex with genomic RNA or DNA and sometimes enzymes needed for the first steps of viral replication. (msdmanuals.com)
Classification of viruses is principally according to their genome sequence taking into consideration nature and structure of their genome and their method of replication, but not according to the diseases they cause (see International Committee on Taxonomy of Viruses (ICTV), 2021 release ). (msdmanuals.com)
Positive-sense RNA viruses possess a single-stranded RNA genome that can serve as messenger RNA (mRNA) that can be directly translated to produce an amino acid sequence. (msdmanuals.com)
Certain single-strand, (+) sense RNA viruses termed retroviruses use a very different method of replication. (msdmanuals.com)
Continual genetic mutation and recombination, a requirement for replication in macrophages, and persistent infection (carrier animals) despite an active immune response are characteristics shared by viruses in this family. (extension.org)

VLPs self-assembled into empty shells can be used safely as vaccines without the risk of replication or infection due to the lack of viral nucleic acid. (nature.com)
Of course the story book version of viral infection involves a single infective viral particle doing the job. (virology.ws)
A single particle, even a few hundred or thousand, are not enough - viral infection is quite inefficient in an animal host. (virology.ws)
The degree of visible damage to cells caused by viral infection varies with type of virus, type of host cells, multiplicity of infection (MOI) and other factors. (asm.org)
The peak viral load of SARS‑CoV‑2 is estimated to occur ~10 days following fever onset, causing patients in the acute stage to be the primary infection source. (spandidos-publications.com)
Nuclear extracts prepared from uninfected cells as well as cells harvested at 5 and 12 hr after infection contained similar levels of MLTF/USF footprint activity, therefore the lack of a detectable interaction early after infection is not due to reduced levels of the factor early in the viral growth cycle. (princeton.edu)
Of particular interest was the finding that PC-positive DBs induced an antibody response that blocked the infection of fibroblasts by a PC-positive viral strain more efficiently than sera following immunizations with PC-negative particles. (asm.org)
Using a subviral particle vaccine candidate, we show here that one protein complex of HCMV, termed the pentameric complex (PC), enhances the neutralizing antibody response against viral infection of different cell types. (asm.org)
I think it would be more advantageous to identify every protein structure in the viral envelope, make a variety of epitopes from short sequences, and test them as immunogens that can prevent infection of a variety of cell types. (macresource.com)
An interferon regulatory factor that is expressed constitutively and undergoes POST-TRANSLATIONAL MODIFICATION following viral infection. (lookformedical.com)
In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD (PubMed:12414943). (nih.gov)
Approximately 2.4 million people in the US are coping with chronic Hepatitis C. The Hepatitis C virus (HCV) is a viral infection that affects the liver. (ehg.health)
Development of DDX5 inhibitors will help to delineate the complex roles of DDX5 and other DEAD-Box helicase in cancer and viral infection and hopefully lead to development of improved therapeutic strategies. (bellbrooklabs.com)
Replication may be categorized into immediate early, delayed early, and late gene expression based on time of synthesis after infection. (medscape.com)
The pathogenesis of hairy leukoplakia is clearly complex, potentially requiring a convergence of factors including EBV co-infection, productive EBV replication, EBV genetic evolution, expression of specific EBV "latent" genes, and immune escape. (medscape.com)
[ 3 , 4 ] Langerhans cells are the antigen-presenting immune cells that are required for an immune system response to the viral infection and their deficiency may permit EBV to persistently replicate and escape immune recognition. (medscape.com)

The host cell must provide the energy and synthetic machinery and the low- molecular-weight precursors for the synthesis of viral proteins and nucleic acids. (wikipedia.org)
ISGs include several genes encoding ARTs, enzymes that catalyze ADP-ribosylation of proteins and nucleic acids using NAD + as cofactor. (fsu.edu)

Nsp3 and Nsp5 are essential for viral replication and represent well-characterized drug targets among coronaviruses. (elifesciences.org)

Technical virology questions about ERVs and RNA vs. DNA viral rates of mutation? (fluther.com)
Homology of HAM1h with cellular Maf/HAM1 NTP pyrophosphatases suggests that HAMh1 might intercept non-canonical NTPs to reduce mutation rates of viral RNA. (ictv.global)
HAMh1 might intercept non-canonical NTPs to reduce mutation rates of viral RNA. (ictv.global)
The book is concerned with molecular biology studies, macromolecules and the macromolecular mechanisms found in living things, such as the molecular nature of the gene and its mechanisms of gene replication, regulation, mutation, post transcriptional modifications and expression. (agroacademics.com)

The diagram above shows a small subset of the viral genomes that are present in a virus stock. (virology.ws)
Until recently it was not possible to know the sequences of all the viral genomes present in a population such as that illustrated in the figure. (virology.ws)
While viral genomes can be comprised of RNA or DNA, SARS-CoV-2 is encoded by an RNA molecule. (qiagen.com)
In both genomes, there was evidence for interaction of the sub-terminal bulge loop of SL9266 and sequences around nucleotide 9110, though the replication phenotype of genomes bearing mutations that disrupted this interaction was fundamentally different. (ox.ac.uk)

This phase involves the multiplication of the genome and the synthesis of the viral messenger RNA (mRNA). (bibalex.org)
The viral genome has to both replicate itself and become transcribed into viral mRNA molecules. (bibalex.org)
The viral mRNA can then be translated by the host cell's ribosomes into viral structural components and enzymes need for replication and assembly of the virus. (bibalex.org)
4 On the other hand, DDX5 binds the SARS-CoV helicase to enhance viral proliferation, facilitates HIV-1 mRNA export, and enhances influenza virus polymerase activity. (bellbrooklabs.com)

The viral genome (total genetic information) can be RNA or DNA. (icr.org)
In stark contrast, virtually no attention has been paid to the physical provenance of those nearest genetic relatives, its presumptive ancestors, which are two viral sequences named BtCoV/4991 and RaTG13. (pearltrees.com)
There they introduce their genetic information in the form of the nucleic acids DNA or RNA. (uni-bonn.de)
Our nucleic acid tests detect the Hepatitis C virus' genetic material, RNA. (ehg.health)
Once the provirus is integrated into the host cell DNA, it is transcribed using typical cellular mechanisms to produce viral proteins and genetic material. (msdmanuals.com)
A nucleic acid genome (their genetic material: DNA or RNA). (hearone.in)
Research has produced a great deal of information concerning the PRRS virus genome and viral proteins, but a genetic basis for the variation in virulence and antigenicity among PRRS virus isolates has not yet been identified. (extension.org)
These DNA and RNA fragments may be mistaken for the genetic material of viral invaders, triggering immune system reactions in multiple body systems that cause severe brain dysfunction (encephalopathy), skin lesions, and other signs and symptoms of Aicardi-Goutières syndrome. (medlineplus.gov)

Hepatitis B virus deoxyribonucleic acid. (cdc.gov)
This commentary discusses the use of nucleic acid (deoxyribonucleic acid and ribonucleic acid) vaccines against viral infections and pandemic-like settings. (canada.ca)

These host cell receptors are normal surface molecules involved in routine cellular function, but since a portion of a molecule on the viral surface resembles the chemical shape of the body's molecule that would normally bind to the receptor, the virus is able to attach to the host cell's surface. (bibalex.org)
The viral genome directs the host cell's metabolic machinery (ribosomes, tRNA, nutrients, energy, enzymes) to synthesize viral enzymes and viral parts. (bibalex.org)

Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
It appears to mainly prevent the release of infectious viral nucleic acid into the host cell by interfering with the function of the transmembrane domain of the viral M2 protein. (nih.gov)
However, increased ACE2 expression may upregulate the viral load indirectly by increasing the baseline level of infectious virus particles. (spandidos-publications.com)
Compatible with multiplexed RT-PCR and NGS-based assays, AccuPlex custom recombinant virus materials are constructed with a replication-defective mammalian virus, producing a safe, non-infectious material (Figure 1). (seracare.com)

There are no mutations in the consensus sequence, even though every viral genome contains mutations. (virology.ws)
Using amplicons optimized for short-read platforms, the QIAseq DIRECT SARS-CoV-2 Kit uses an overlapping and redundant targeting approach to reduce the risk of drop outs caused by novel viral mutations. (qiagen.com)

Target amplification of SARS-CoV-2 requires both reverse transcription and whole genome enrichment of the viral RNA. (qiagen.com)
The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing. (genomeweb.com)
Samples with positive antibody confirmation results were then tested with a nucleic acid amplification test for hepatitis C virus RNA. (cdc.gov)

The consequence of a quasispecies is that most viral infections are initiated not by a single virion, but a population of particles. (virology.ws)
In turn the expression of IFN-stimulated genes (ISGs) is induced, allowing the host to react swiftly to viral infections. (fsu.edu)
The potential advantages of the nucleic acid vaccines over conventional vaccines are presented, and the nucleic acid vaccines currently in development against viral infections and the challenges these vaccines face entering clinical trial are discussed. (canada.ca)
In the past few decades, there has been rapid spread of numerous severe viral infections, including human immunodeficiency virus (HIV), influenza A, severe acute respiratory syndrome (SARS), Ebola and Zika. (canada.ca)
These results will be of interest to virologists interested in studying the alterations in tRNA modifications, host methyltransferases, and viral infections. (elifesciences.org)
This is why pigs infected with PRRS virus often have severe secondary infections with any of a variety of bacterial or viral pathogens including, Mycoplasma hyopneumonia, Salmonella choleraesuis, Haemophilus parasuis, Streptococcus suis, Pasteurella multocida, influenza virus, and others. (extension.org)

This phase leads to the release of the viral genomic nucleic acid from the protein coat. (bibalex.org)
Besides these enzymatic activities which are essential for replication of viral genomic RNA, this protein also participates in other aspects of the viral life cycle. (caister.com)

the virus consists of nucleic acid, either DNA or RNA, and a protein coat that envelops that nucleic acid. (bibalex.org)
They have nucleic acid core surrounded by protein coat. (brainkart.com)
The virion is made up of two constituents, a protein coat called capsid and a core called nucleic acid . (brainkart.com)

During this phase, the Virion, which, according to theFreeDictionary.com, is "a complete viral particle, consisting of RNA or DNA surrounded by a protein shell and constituting the infective form of a virus", enters the host cell either through endocytosis (1) or membrane fusion. (bibalex.org)
The virion , which is the infective virus particle, consists of nucleic acid (which carries the genome) surrounded by layers of protein that create a protective membrane called a capsid. (mometrix.com)
With budding, each viral particle is released individually. (mometrix.com)
Together these act together like a Velcro so it is very hard to break up the self-assembled viral particle. (hearone.in)

For example, DDX5 inhibits myxoma virus replication, represses hepatitis B virus (HBV) minichromosomal function, and enables STAT1 signaling/the IFN innate immune response in HBV-infected cells. (bellbrooklabs.com)

During this phase, the whole viral capsid is removed. (bibalex.org)
This means that the capsid degrades and releases nucleic acid into the cytoplasm of the host cell. (mometrix.com)

During the time that synthesis of viral components is occurring in the infected cell, the cell undergoes characteristic biochemical and morphological changes. (asm.org)

The nucleic acid of virions contains either RNA or DNA, depending on the type of virus. (mometrix.com)

His research expanded to the mechanism regulating the transcription of cellular and viral genes and characterized the protein SRSF1 as a strong inhibitor of HIV-1 replication. (fau.edu)
The compound is a potent inhibitor of HIV replication at low concentrations, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. (bvsalud.org)

For a virus to infect a host cell, that cell must have receptors for the virus on its surface and also be capable of supporting viral replication. (bibalex.org)
Emerging evidence demonstrates that some mono-ARTs function as PAMP receptors and modify both host and viral proteins relevant for viral replication. (fsu.edu)
DDX5 (Dead-Box 5 or p68) is a member of a family of 37 "DEAD-Box" ATP-dependent RNA helicases that play a role in nearly all aspects of RNA processing and also act as nucleic acid recognition receptors for viral immunity. (bellbrooklabs.com)

VLPs are highly immunogenic and can induce elevated titers of neutralizing antibodies, even without adjuvants, thanks to the maintenance of the native conformation of viral proteins presenting repetitive epitopes to the host immune system cells. (nature.com)

Molecules that result from the replication of those described in (1) or (2) above. (uwm.edu)
ALL recombinant or synthetic nucleic acid molecules or materials that contain these molecules must be decontaminated before disposal. (uwm.edu)
The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. (microbiologyresearch.org)
Interferons do a number of things, but in virally infected cells, they signal the cells to express antiviral mechanisms such as downregulating protein synthesis, expression of RNAses and DNAses to degrade nucleic acids, induction of host cell death (kill the vehicle) and expression of MHC molecules so that a more robust immune response can ensue. (fluther.com)
The alcohol acts to disrupt RNA molecules in the virus, preventing viral replication (in other words it blocks the virus from making copies of itself). (hearone.in)
In other words, molecular biology is an interdisciplinary approach to understanding biological functions and regulation at the level of molecules such as nucleic acids, proteins, and carbohydrates. (agroacademics.com)

Researchers found that the RNA sequences they analyzed contained a signature gene called RdRp that codes for RNA-dependent RNA polymerase-a complex enzyme designed to catalyze replication. (icr.org)
Retroviruses have been shown to efficiently delete sequences between repeats as a consequence of the template switching ability of the viral reverse transcriptase. (peerj.com)
Although HIV-1 based gene transfer vectors lack most of the viral coding sequences, it retains a small portion of the gag sequence, and also contains a 5′ splice donor site upstream of gag and frequently a 3′ splice acceptor site further downstream. (peerj.com)

Amantadine inhibits the replication of influenza A virus isolates from each of the subtypes, i.e. (nih.gov)
The influenza virus snatches the cap part of the mature host RNA to start viral replication. (uni-bonn.de)
The activity of MTr1 is thus essential for the replication of the influenza virus in the cell. (uni-bonn.de)
Among thousands of candidates, we were able to identify a molecule that inhibits MTr1 in human lung explants and also in vivo in mice, curtailing influenza replication," reports Prof. Hiroki Kato, a member of the Cluster of Excellence ImmunoSensation2 at the University of Bonn. (uni-bonn.de)
Generally nucleic acid is present as a single unit but in wound tumour virus and in influenza virus it is found in segments. (brainkart.com)

Their presence can be detected visually only by hemadsorption or interference, in which infected cell cultures showing no CPE inhibit the replication of another virus subsequently introduced into the cultures, or in situ by viral antigen or nucleic acid detection. (asm.org)

Phylogenetic and amino acid comparison of highly and less neuroinvasive lineage 2 strains demonstrated that did not correlate with lineage, which suggests that highly the nonstructural genes, especially the nonstructural pro- and less neuroinvasive phenotypes exist in both lineages tein 5 gene, were most variable. (cdc.gov)
These results indicate that DNA replication participates in the regulation of adenovirus late gene expression by facilitating the binding of a transcription factor to the major late promoter. (princeton.edu)
2017) Tat is a multifunctional viral protein that modulates cellular gene expression and functions. (fau.edu)
Lentiviral gene delivery systems consist of packaging (or helper) plasmids that code for viral structural and regulatory proteins, and a gene transfer vector that contains the transgene expression cassette ( Srinivasakumar, 2001 ). (peerj.com)

Compared to wildtype human cells, TRMT1-deficient human cells infected with SARS-CoV-2 exhibit reduced levels of intracellular viral RNA. (elifesciences.org)

The non-structural protein NSP2 of rotavirus, which exhibits nucleoside triphosphatase, single-stranded RNA binding, and nucleic-acid helix-destabilizing activities, is a major component of viral replicase complexes. (nih.gov)
It bears a molecular mass of 69 kDa and is endowed with multiple functions including proteolytic processing, nucleic acid duplexes unwinding, nucleoside triphosphatase (NTPase) and RNA nucleoside 5' triphosphatase (RTPase). (caister.com)

The viral non-structural protein 3, which encodes a mono-ADP-ribosylhydrolase, antagonizes cellular mono-ADP-ribosylation and reactivates the protease. (fsu.edu)
The non-structural protein 3 (NS3) of flaviviruses is the second most conserved amongst the viral proteins. (caister.com)
A twist in the tail: SHAPE mapping of long-range interactions and structural rearrangements of RNA elements involved in HCV replication. (ox.ac.uk)

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. (nih.gov)
The nucleic acids may be single stranded or double stranded. (brainkart.com)

By inhibiting Nsp5 proteolytic activity, Paxlovid reduces viral replication and disease severity in patients with COVID-19. (elifesciences.org)

These protein subunits can assemble into virus particles without viral RNA genome in mammalian cells to study virus-cell entry 2 and to develop vaccines 3 . (nature.com)
Researchers also use cells as biological test tubes to grow viral stocks. (the-scientist.com)
MTr1-deficient cells or cells treated with MTr1 inhibitors do not lead to IAV replication. (uni-bonn.de)
The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
Two yeast strains were used, differing in the biogenesis of peroxisomes, the organelles supplying the membranous vesicular environment in which CymRSV RNA replication takes place in infected plant cells. (microbiologyresearch.org)
DI RNA replication occurred in yeast cells, as demonstrated by the presence of monomers and dimers of positive and negative polarities. (microbiologyresearch.org)
DI RNA replication also took place in yeast cells devoid of peroxisomes. (microbiologyresearch.org)
It is suggested that replication in these cells was targeted to the endoplasmic reticulum. (microbiologyresearch.org)
One major mechanism used by multicellular organisms (such as humans) is the expression of cytokines by immune cells to signal to infected cells to shut of viral replication. (fluther.com)
RNA-DNA hybrids are formed during DNA copying (replication) and are found in all cells. (medlineplus.gov)

Labelling with BrUTP showed that peroxisomes were the sites of nascent viral synthesis, whereas in situ hybridization indicated that DI RNA progeny were diffused throughout the cytoplasm. (microbiologyresearch.org)

In certain cases, amantadine is also known to prevent virus assembly during virus replication. (nih.gov)
Empirical validation of viral quasispecies assembly algorithms: state-of-the-art and challenges. (genomeweb.com)
The fifth stage is assembly , during which the pieces of nucleic acid and proteins come together to form new viral particles, which mature until they are ready for the sixth and last stage, release . (mometrix.com)
The connections between these three component parts provide the structure of the virus, but those connections are weak - there are no covalent bonds in action that would provide a more stable structure.the viral self-assembly is based on weak "non-covalent" interactions between the proteins, RNA and lipids. (hearone.in)

By using sequence alignment of herpesviruses homologs, we identified conserved amino acids in the putative pUL105 ATP binding site and in the putative pUL70 zinc finger pattern. (frontiersin.org)
Mutational analysis of several of these amino acids both in pUL105 and pUL70, proved that they are crucial for viral replication. (frontiersin.org)
We also constructed, by homology modeling, a theoretical structure of the pUL105 N-terminal domain which indicates that the mutated conserved amino acids in this domain could be involved in ATP hydrolysis. (frontiersin.org)
DEAD-Box helicases are named for the core Asp-Glu-Ala-Asp (DEAD) amino acid sequence they share. (bellbrooklabs.com)
DDX5 is a 614 amino acid protein consisting of an N-terminal Q motif, an ATP binding Rec A-like domain, a hinge region, a Rec A-like helicase domain, and a C-terminal RGS-RGG motif. (bellbrooklabs.com)

Also, are you aware of any cases of someone lacking the enzyme to stop the viral DNA expression, and thus they have a problem? (fluther.com)

The size of the entire SARS-CoV-2 genome is under 30 kb and can be mixed with host RNA when isolating from a human sample, making it challenging to reconstruct the whole viral genome. (qiagen.com)

A steady-state population of a viral quasispecies consists of a vast number of particles. (virology.ws)

At present, PRRS is the most economically important viral disease of swine in North America, with production costs to pig producers in the United States estimated at $560 million annually. (extension.org)

Viral diseases are the most common type of infective disorders. (mometrix.com)

The viral pentameric protein complex consisting of glycoprotein H (gH)/gL/UL128-131A (PC) is considered to be an important vaccine component. (asm.org)
The study is a "proof of concept that a pan-coronavirus vaccine in humans is possible", says David Martinez, a viral immunologist at the University of North Carolina at Chapel Hill. (macresource.com)

Abnormal functioning of this complex may disrupt transcription, DNA replication, DNA repair, cell death (apoptosis), or other processes. (medlineplus.gov)

However, in either cases, replication of the viral genome is highly dependent on a cellular state permissive to DNA replication and, thus, on the cell cycle. (wikipedia.org)
In 2003, Dr. Caputi was hired as an Assistant Professor at FAU where he continued his work on the molecular biology of HIV-1 and the mechanisms regulating cellular and viral splicing. (fau.edu)
During his tenure at FAU, Dr. Caputi has developed novel techniques to study RNA - RNA binding proteins interactions and made important contributions to our understanding of how cellular proteins modulate the replication of the HIV-1 genome. (fau.edu)
The RNase H2 complex is also thought to be involved in DNA replication, error repair, and other cellular processes, including helping to prevent inappropriate immune system activation. (medlineplus.gov)

Once delivered into the host cell, the incoming positive-strand viral RNA genome is first translated by host ribosomes into two overlapping polyproteins, pp1a and pp1ab. (elifesciences.org)

We propose that, SL9266 may contribute a switch function that modulates the mutually incompatible translation and replication events that must occur for replication of the positive-strand RNA genome of HCV. (ox.ac.uk)

The panel consists of approximately 550 primers for creating 425 amplicons, covering the entire SARS-CoV-2 viral genome. (qiagen.com)

Virologists now understand that virus populations are not made of a single member with a defined nucleic acid sequence. (virology.ws)
This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. (nih.gov)

More recently, Dr. Caputi's research has expanded through collaborative research grants to the development of microfluidic diagnostic devices for the detection of multiple viral species from blood and other bodily fluids. (fau.edu)

A Q-beta phage population is in a dynamic equilibrium with viral mutants arising at a high rate on the one hand, and being strongly selected against on the other. (virology.ws)

The HCMV helicase-primase complex (pUL105-pUL102-pUL70) is essential for viral DNA replication and could thus be a relevant antiviral target. (frontiersin.org)

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