AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceNamed GroupsHealth CareGeographicals
beta 2-Glycoprotein IAntibodiesAntibody SpecificityAntiphospholipid SyndromeAntibodies, AnticardiolipinAntibodies, MonoclonalAntibodies, ViralAntibodies, BacterialScleroderma, SystemicAutoantibodiesAntibodies, AntiphospholipidAntibody FormationDNA Topoisomerases, Type IHuman T-lymphotropic virus 2Antibody AffinityLupus Coagulation InhibitorAntibodies, NeutralizingEpitopesDeltaretrovirus InfectionsAntibodies, AntinuclearFluorescent Antibody TechniqueGlycoproteinsCross ReactionsHTLV-I InfectionsEnzyme-Linked Immunosorbent AssayImmunoglobulin GDeltaretrovirusAntigen-Antibody ReactionsImmunoglobulin MDeltaretrovirus AntibodiesQ FeverBinding Sites, AntibodyCoxiella burnetiiAntibodies, Anti-IdiotypicLupus Erythematosus, SystemicHuman T-lymphotropic virus 1Paraneoplastic SyndromesHIV AntibodiesMolecular Sequence DataAntibodies, NeoplasmAntibodies, ProtozoanIsoantibodiesAmino Acid SequenceRNA Polymerase ICardiolipinsHistocompatibility Antigens Class IImmunoblottingHuman T-lymphotropic virus 3AutoantigensAntibodies, FungalNeutralization TestsCells, CulturedAntibodies, BispecificSingle-Chain AntibodiesSimian T-lymphotropic virus 1Mice, Inbred BALB CAntibodies, BlockingCell LineAntigen-Antibody ComplexBlood DonorsThrombosisImmunoglobulin Fab FragmentsAntibodies, HeterophileAntibodies, CatalyticSkinSeroepidemiologic StudiesImmunoenzyme TechniquesT-LymphocytesRabbitsImmunoglobulin AAntibodies, Monoclonal, HumanizedFluorescent Antibody Technique, IndirectGenes, pXBlotting, WesternHybridomasImmune SeraAntigens, SurfaceAntigens, ViralEpitope MappingImmunizationPrimate T-lymphotropic virus 3Gene Products, taxRetroviridae InfectionsAntigensBase SequenceAntigens, BacterialB-LymphocytesImmunization, PassiveRecombinant ProteinsImmunoassayImmunoglobulin FragmentsMolecular WeightImmunohistochemistryGene Products, envRetroviridaeViral Envelope ProteinsRadioimmunoassayRadioimmunoprecipitation AssaySerologic TestsElectrophoresis, Polyacrylamide Gel
AntigensMonoclonal antibodyInfectionSerumRetrovirusAssayDetectionAssaysProviralSyphilisGp46Surface antigenPolyclonalCloneReactivityLymphocytePathogenesisAIDSProvirusMyelopathySpecificityLymphotropicDetectsVitroRecombinantAdultPhosphate-bufferLysateLymphomaVirusProteinEpitopes boundIndirect immunofluorescenceSerologicPrevalenceVirusesTitersSpecificBoundPatients with viralInfectiousInfectionsExperimentallyDetectableAntigenicInfectivityProteinsPlasmaVaccinesGeneConfirmatoryPresenceEpidemiologyMice
Antigens9
  • The position of bands on the nitrocellulose strips allows this antibody reactivity to be associated with specific viral antigens. (cdc.gov)
  • During incubation, if HIV-1 antibodies are present in the specimen, they will bind to the viral antigens bound to the nitrocellulose strips. (cdc.gov)
  • If antibodies to any of the major HIV-1 antigens are present in the specimen in sufficient concentration, bands corresponding to the position of one or more of the following HIV-1 proteins (p) or glycoproteins (gp) will be seen on the nitrocellulose strip: p17, p24, p31, gp41, p51, p66, gp120, gp160 (number refers to apparent molecular mass in kilodaltons). (cdc.gov)
  • By aligning antibodies of distinct specificities in a matrix-like manner, scientists are able to detect and quantify multiple antigens in one simultaneous attempt. (wikipedia.org)
  • Diagnosis of the causative pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is based on reverse transcription-PCR (RT-PCR) to detect viral nucleic acid or serologic assays to detect SARS-CoV-2 antigens in early stages of disease ( 1 , 2 ). (cdc.gov)
  • We tested all 68 serum samples by using commercially available ELISAs from EUROIMMUN ( https://www.euroimmun.com ) that rely on different antigens and antibody classes: SARS-CoV-2 nucleocapsid (N) antigen (IgG), spike 1 (S1) subunit (IgG and IgA), and Middle East respiratory syndrome coronavirus (MERS-CoV) S1 (IgG). (cdc.gov)
  • Most patients with primary Sjogren syndrome have two specific antibodies: against Ro (SS-A) and La (SSB) antigens. (medscape.com)
  • These tumor specific antigens are the result of mutations that cancer cells undergo, and in many cases that involve viral carcinogens, viral antigens. (oncolink.org)
  • and human T -cell antigens recognized by certain monoclonal antibodies on both immature and mature T -cells. (science-connections.com)
Monoclonal antibody6
  • This murine monoclonal antibody reacts with Human T-Lymphotropic Virus Type I (HTLV-I) surface protein, gp46. (zeptometrix.com)
  • In fact, this type of antigen was the target of the first monoclonal antibody therapy for a malignancy (B-cell lymphoma) in 1982. (oncolink.org)
  • A few months ago, the company merged with monoclonal antibody developer BioClonetics, which is developing optimized antibodies for a variety of diseases including HIV and COVID-19. (insiderfinancial.com)
  • In consistent with the promising therapeutic effect of monoclonal antibody mogamulizumab, targeting another GPCR CCR4, in treating aggressive HTLV-1-driven adult T-cell leukemia-lymphoma, pre-clinical study demonstrated that CCR1 blockade not only directly killed tumor cells, but also enhanced cytotoxic cell expansion irrespective of molecular subtypes or LME categories. (bioengineer.org)
  • We developed a monoclonal antibody (5D2) to FTC and demonstrated its high specificity and physiologically relevant linear range of detection in a competitive enzyme immunoassay (EIA). (cdc.gov)
  • Thus, this monoclonal antibody is a key reagent that will enable simple and low-cost lateral flow assays and EIAs for adherence monitoring. (cdc.gov)
Infection19
  • Human Immunodeficiency Virus (HIV) Infection Human immunodeficiency virus (HIV) infection is a viral infection that progressively destroys certain white blood cells and is treated with antiretroviral medications. (msdmanuals.com)
  • HTLV-1-associated myelopathy/tropical spastic paraparesis is more common among women because HTLV-1 infection is more common among women. (msdmanuals.com)
  • A similar disorder can result from infection with a similar virus, human T-lymphotropic virus 2 (HTLV-2). (msdmanuals.com)
  • In later stages of disease, antibody-based serologic testing can complement diagnosis of SARS-CoV-2 infection. (cdc.gov)
  • Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B. (cusabio.com)
  • Most often infection is chronic and indolent with little viral replication, exceeded by larger amounts of latent, integrated provirus. (diseases-illness.com)
  • On the other hand, zero of six rabbits inoculated with 729.ACH.p13 cells didn't create viral infection, whereas six of six rabbits inoculated with wild-type HTLV-1-expressing cells (729.ACH) were contaminated seeing that measured by antibody replies, proviral fill, and HTLV-1 p19 matrix antigen creation from former mate vivo-cultured PBMC. (arcticcirclescotland.com)
  • HTLV-1 infection was produced in rabbits inoculated with ACH-transfected, irradiated PBMC. (wustl.edu)
  • HTLV-1 infection is usually diagnosed by serology assays detection of HTLV-1 specific IgG or IgM antibodies. (meridianbioscience.com)
  • Preventing Ebola infection through a vaccine is now a major global research focus and researchers from the Scripps Research Institute, led by Laura Walker, aimed to gain further knowledge on the role of antibodies in protection against Ebola. (immunopaedia.org.za)
  • At only 3 months post infection, 3% of the donor IgG antibodies were specific to the Ebola envelope glycoprotein (GP) and bound strongly to the GP. (immunopaedia.org.za)
  • The epitopes bound by these antibodies could potentially be used in anti-Ebola immunogens since they can be targeted in natural infection. (immunopaedia.org.za)
  • Developing and testing rationally-designed vaccines to prevent HIV infection, and also using the HIV envelope epitopes bound by human monoclonal antibodies to guide our vaccine design and test the vaccines in rabbits and monkeys to screen which vaccines are most immunogenic and should proceed to human trials. (mssm.edu)
  • Since the JHK-3 cell line also contains Epstein-Barr virus DNA and nuclear antigen and a 196-nm viral particle of unusual morphology, this particle will be identified and its relationship determined, if any, to JHK viral infection. (ncf-net.org)
  • Respiratory syncytial virus (RSV) is the most frequent cause of lower respiratory tract infection in infants and children and the second most common viral cause of pneumonia in adults. (medscape.com)
  • Vertical transmission is a relevant concern in Brazil, as the blotting (WB) and/or polymerase chain reaction (PCR) assays prevalence of HTLV-1 infection in pregnant women ranges from as a confirmatory step. (bvsalud.org)
  • A recent study evaluated the performance of commercially available serological screening tests for HTLV-1 infection in Brazil, i.e. (bvsalud.org)
  • genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)
  • These tests detect antibodies to HCV (anti-HCV) and are used for screening and diagnosing HCV infection. (medscape.com)
Serum8
  • The Cambridge Biotech HIV-1 Western Blot Kit, when used as directed, will detect antibodies to HIV-1 when present in human serum or plasma. (cdc.gov)
  • The antibody is of the IgG1 subclass and has been purified from serum-free culture supernatant by ammonium sulfate precipitation. (zeptometrix.com)
  • The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.com)
  • Serum samples also were tested by using commercially available ELISA kits ( Euroimmun ) against the Zika virus (ZIKV) nonstructural protein 1 (NS1) antigen (IgG), the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) (IgG), and the EBV viral capsid (CA) antigen (IgM and IgG), as well as real-time PCR tests (TIB MOLBIOL, https://www.tib-molbiol.com ) for all human pathogenic Plasmodium species, EBV, and cytomegalovirus (CMV). (cdc.gov)
  • Serum samples were tested for total anti-HAV antibodies using the Enzyme-Linked Immunosorbent Assay (ELISA) kit. (razavihospital.ir)
  • Serum specimens obtained were tested for the presence of HBV, HCV, HDV and HTLV-1&2 infections. (biomedcentral.com)
  • Line immunoassay for the confirmation and discrimination of antibodies to Human T-cell Lymphotrophic Virus type I (HTLV-I) and type II (HTLV-II) in human serum and plasma. (launchdiagnostics.com)
  • Serum samples were tested for viral reduced the risk of transfusion-transmitted markers using commercially-available diseases, although it has increased the cost enzyme-linked immunosorbent assays for of providing safe blood [ 3 ]. (who.int)
Retrovirus6
  • Individual T-lymphotropic pathogen type 1 (HTLV-1) is certainly classified being a complicated retrovirus and an associate from the genus as well as the 3 lengthy terminal do it again that encodes the regulatory proteins Taxes and Rex, aswell as several non-structural accessories proteins, p12I, p27I, p13II, and p30II (12, 16, 28, 30). (arcticcirclescotland.com)
  • We therefore tested whether oleandrin or an extract from N. oleander could also inhibit the infectivity of the human T-cell leukemia virus type-1 (HTLV-1) -a related enveloped retrovirus and emerging tropical infectious agent. (longdom.org)
  • These findings prompted us to investigate whether oleandrin could similarly inhibit another related retrovirus, the HTLV-1. (longdom.org)
  • HTLV-1, the first human retrovirus identified, causes adult T cell leukemia. (meassociation.org.uk)
  • The second human retrovirus, HTLV-2 has not been associated with any disease. (meassociation.org.uk)
  • A novel retrovirus, human T -celllymphomaleukemia virus (HTLV), has been isolated from the fresh and cultured cells of two of these patients. (science-connections.com)
Assay5
  • Serologic testing using the enzyme-linked immunosorbent assay (1) and the Western blot technique (2) for antibody to the human T-lymphotropic virus type III (HTLV-III) has been done, with specific informed consent, on 40 HCWs enrolled in the surveillance system. (cdc.gov)
  • Inoculated rabbits seroconverted to positivity for antibodies against HTLV-1 and had steady or rising HTLV-1 enzyme-linked immunosorbent assay antibody titers. (wustl.edu)
  • The researchers used a live virus plaque reduction neutralization assay to test how well the antibodies were able to neutralize Ebola strains. (immunopaedia.org.za)
  • Furthermore, 146 sera samples from prostate tumor patients were tested for XMRV Gag and Env antibodies using a newly developed ELISA assay. (biomedcentral.com)
  • Encouragingly, a more recently developed serological to evaluate intrathecal synthesis of specific and total antibodies, confirmatory assay (INNO-LIA, HTLV-I/II) yielded results for which represent specific inflammatory process markers in the most samples considered indeterminate or untypeable under CNS10. (bvsalud.org)
Detection6
  • All specimens were tested using the Synthetic Peptide Enzyme Immunoassay (EIA) (Genetic Systems HIV-1/HIV-2 Peptide EIA) for the detection of antibody to human immunodeficiency virus type 1 or type 2 (HIV-1 or HIV-2) or both (Bio-Rad Laboratories, Redmond, WA). (cdc.gov)
  • Combination antiretroviral therapies potently suppress plasma human immunodeficiency virus (HIV) RNA levels, although many patients who initially achieve suppression below the limits of detection experience viral relapse to detectable levels. (diseases-illness.com)
  • Mikrogen ampliCube Respiratory Viral Panel 2 Real-time RT-PCR for detection of Parainfluenza 1-4, Bocavirus More information on Mikrogen ampliCube infectious disease assays can be. (launchdiagnostics.com)
  • This section uses Real-time PCR techniques for the detection of viral nucleic acid in clinical samples. (hslpathology.com)
  • Antibody-based testing for emtricitabine (FTC), a critical component of pre-exposure prophylaxis and antiretroviral therapy would provide low-cost detection for clinical monitoring to improve adherence. (cdc.gov)
  • The Rapid Screening Test (RST) was used for the detection of Dengue-specific IgG and IgM antibodies. (bvsalud.org)
Assays5
  • We therefore investigated whether oleandrin or a N. oleander extract could inhibit virus transmission from a GFP-expressing HTLV-1+ lymphoma T-cell-line to huPBMCs in co-culture assays. (longdom.org)
  • This present study describes the development and use of sensitive PCR and RT-PCR assays to test DNA and RNA from 589 PCa tumor samples obtained from the Charité hospital in Berlin (Germany) for the presence of proviral XMRV DNA and corresponding viral transcripts. (biomedcentral.com)
  • HTLV-1 proviral load (PVL) in It is highly important to implement an accurate and cost- the blood and/or CSF is also useful in establishing diagnosis effective strategy for laboratory diagnosis and systematically and, when combined with other tests, such as evaluation of evaluate the performance of both screening assays and intrathecal synthesis of HTLV-1 specific antibodies, may also aid confirmatory testing. (bvsalud.org)
  • Molecular assays: These detect viral nucleic acid and can be qualitative or quantitative. (medscape.com)
  • Qualitative tests only detect the presence of HCV RNA (specificity, approximately 98%) whereas the quantitative assays detect the viral load in IU/mL (specificity, approximately 98-99%) and provide adequate information that guides clinicians to manage and monitor treatment outcome. (medscape.com)
Proviral2
  • Although initial research reported that HTLV-1 ORF II was dispensable for viral infections in vitro (15, 37), disruption of pX ORF II within an HTLV-1 proviral clone that blocks appearance of full-length p30II by itself or both p30II and p13II significantly decreased viral infectivity and web host humoral response in rabbits (2, 39). (arcticcirclescotland.com)
  • This transformant culture was shown by flow cytometric analysis to be a CD4 + CD25 + T-lymphocyte population containing, as determined by Southern blot analysis, at least three integrated HTLV-1 proviral copies. (wustl.edu)
Syphilis2
  • Negative for Hepatitis B (antigen), Hepatitis C (antibodies), HIV I and II (antigen and antibodies), HTLV I/II and syphilis. (geticadiagnostics.com)
  • It is extremely important to minimise the risk of infecting the recipient, therefore all viral screening carried out on the donors (Hep B Surface Antigen, Hep B Core Antibody, Hep C, HIV, HTLV 1-2, Syphilis, CMV, Chlamydia and Gonorrhoea) within 3 months of storage of embryos must be negative and sample stored in a screened tank. (fertilityroad.com)
Gp465
  • Anti-HTLV-I gp46 Clone 68/4.11.21 exhibits reactivity with viral lysates using solid-phase enzyme immunoassay and Western blotting. (zeptometrix.com)
  • The antibody immunoprecipitates gp46 surface protein from radiolabeled cell lysates, as well as viral precursor protein. (zeptometrix.com)
  • What's included: One 1 mg vial of Anti-HTLV-I gp46 (clone 68/4.11.21) in phosphate-buffered saline (PBS). (zeptometrix.com)
  • Purified monoclonal antibodies reactive to Gag (p24 and p19) and Env (gp46) are available. (diagnostictechnology.com.au)
  • Anti-HTLV-I gp46 Clone 65/6C2.2.34 exhibits reactivity with viral lysates using solid-phase enzyme immunoassay and immunoblotting. (gentaur-italy.com)
Surface antigen1
Polyclonal1
Clone3
  • A individual 729 B-cell range that expresses a molecular clone of HTLV-1 mutated to selectively ablate p13II appearance (729.ACH.p13) was produced and compared for infectivity with 729 B cells expressing the wild-type molecular clone (729.ACH). (arcticcirclescotland.com)
  • We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
  • This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)
Reactivity1
  • No reactivity with the surface protein of HTLV-II (MoT) has been observed. (zeptometrix.com)
Lymphocyte4
  • The Cambridge Biotech HIV-1 Western Blot Kit is manufactured by Calypte Corporation from HIV-I propagated in an H9/HTLV-IIIb T lymphocyte cell line. (cdc.gov)
  • Our data support growing evidence that HTLV-1 promotes lymphocyte proliferation preceding early Lyl-1 antibody viral spread in lymphoid compartments to establish and maintain prolonged illness. (iahrgrenoble2016.org)
  • The pathogen is certainly connected with lymphocyte-mediated inflammatory illnesses also, such HTLV-1-linked myelopathy-tropical spastic paraparesis (16, 24, 27). (arcticcirclescotland.com)
  • Collectively, these observations indicate a definite function for p13II in HTLV-1 infections and a potential function in HTLV-1-mediated lymphocyte change. (arcticcirclescotland.com)
Pathogenesis3
  • The pathogenesis of ATLL in HTLV-1-infected individuals may differ from one population to another. (oncotarget.com)
  • Therefore, it is important to understand the role antibodies play in the pathogenesis and prevention of Ebola virus. (immunopaedia.org.za)
  • G-protein coupled receptors (GPCRs) are critical targets for viral molecules and correlate with pathogenesis of multiple diseases including inflammation, auto-immune disorder, and cancer. (bioengineer.org)
AIDS3
  • Tests to detect antibody to human immunodeficiency virus (HIV), the virus that causes acquired immunodeficiency syndrome (AIDS), were first licensed by the Food and Drug Administration (FDA) in 1985, primarily as screening tests for blood and plasma donation. (cdc.gov)
  • ELISA, immunofluorescence, immunoblot, etc.) that have been developed for screening persons carrying the viral antibody from patients with overt symptoms of AIDS or AIDS-RELATED COMPLEX. (bvsalud.org)
  • Written before the discovery of HTLV-III/LAV as the AIDS agent, a national case study found: 'Blood from rectal mucosal lesions which are known to be common in homosexual males who engage in rectal intercourse, could contain the infectious agent responsible for this epidemic. (fourwinds10.com)
Provirus2
  • Significantly, we found a higher frequency of hereditary mutations in the ORF-I of provirus from HTLV-1Cinfected people leading to ER retention of p12I, recommending an important function for p12I features in the ER in vivo. (estme.org)
  • Then the integrated viral DNA, or provirus, is passed from parent to offspring. (meassociation.org.uk)
Myelopathy5
  • HTLV-1-associated myelopathy/tropical spastic paraparesis is a slowly progressive disorder of the spinal cord caused by the human T-lymphotropic virus 1 (HTLV-1). (msdmanuals.com)
  • In people with HTLV-1-associated myelopathy/tropical spastic paraparesis, the muscles in both legs gradually become weak. (msdmanuals.com)
  • Introduction Individual T-cell leukemia/lymphoma pathogen type 1 (HTLV-1) may be the etiologic agent of the rare but intense hematopoietic malignancy of T cells, specified adult T-cell leukemia/lymphoma (ATLL), and a intensifying myelopathy thought as HTLV-1Cassociated myelopathy/exotic spastic paraparesis (HAM/TSP). (estme.org)
  • p13II mRNA is certainly expressed in a variety of HTLV-1-contaminated cell lines isolated from scientific sufferers of ATL and HTLV-1-linked myelopathy-tropical spastic paraparesis (5, 36), and circulating cytotoxic lymphocytes particular to ORF II items (i.e., p13II and p30II) have already been discovered both in HTLV-1-contaminated ATL sufferers and in asymptomatic people (34). (arcticcirclescotland.com)
  • however, about 1 in 20 people will develop adult T-cell leukemia/lymphoma or HTLV-1 associated myelopathy (a chronic nervous system disease). (meridianbioscience.com)
Specificity1
Lymphotropic7
  • Determine the presence of Human T-Lymphotropic Virus (HTLV) Type I protein. (zeptometrix.com)
  • The causative agent of ATLL has been identified as the human T cell lymphotropic virus type 1 (HTLV-1) [ 2 - 4 ], which is endemic to Japan, the Caribbean islands, Central and South America, and parts of sub-Saharan Africa [ 5 ]. (oncotarget.com)
  • asked whether human T lymphotropic virus type 1 (HTLV‐1) antibody status is associated with the risk of developing gastric cancer. (diseases-illness.com)
  • Human T-lymphotropic disease type 1 (HTLV-1) is definitely a member of the hinders understanding of the immunopathogenesis of HTLV-1 infections. (iahrgrenoble2016.org)
  • Human T-cell lymphotropic virus-1 (HTLV-1) is part of a group of viruses, including HTLV-I, -II, and -III (also known as HIV-1 and HIV-2). (meridianbioscience.com)
  • When required, additional donation testing is performed, including confirmatory testing, and screening for antibodies to CMV, human T-cell lymphotropic virus (HTLV) and malaria. (lifeblood.com.au)
  • One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
Detects3
  • Phospho-Chk2 (Thr383) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 383. (affbiotech.com)
  • Bax Antibody detects endogenous levels of total Bax. (affbiotech.cn)
  • Phospho-Chk2 (Thr68) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 68. (affbiotech.com)
Vitro4
  • To further examine the binding of the antibodies to GP, they performed negative-stain electron microscopy and these structural studies further confirmed the in vitro findings. (immunopaedia.org.za)
  • These results demonstrated that both oleandrin and the crude phytoextract inhibited the formation of virological synapses and the transmission of HTLV-1 in vitro . (longdom.org)
  • The botanical glycoside, oleandrin, and an extract of Nerium oleander have been shown to prevent the incorporation of the gp120 envelope glycoprotein of HIV-1 into mature virus particles and inhibit viral infectivity in vitro [ 1 ]. (longdom.org)
  • In studies presented here, normal monocytes were infected with the HIV-1/HTLV-III(Ba-L) isolate in vitro and evaluated for chemotactic responsiveness. (johnshopkins.edu)
Recombinant1
  • In addition, we used an ELISA based on recombinant XMRV proteins to screen 146 PCa patient sera for viral Env- and Gag-specific antibodies. (biomedcentral.com)
Adult1
  • HTLV-1 may be the etiological agent of adult T-cell leukemia/lymphoma (ATL), an extremely ASP9521 intense T-cell malignancy (evaluated in guide 43). (arcticcirclescotland.com)
Phosphate-buffer1
  • Each vial contains 100 µg of antibody in phosphate-buffered saline (PBS). (gentaur-italy.com)
Lysate2
Lymphoma2
  • The treatment of HTLV-1+ lymphoma T-cells with either oleandrin or a N. oleander extract did not significantly inhibit viral replication or the release of p19Gag-containing particles into the culture supernatants. (longdom.org)
  • In Brazil, between lymphoma (ATLL) development in approximately 1-5% of 800,000 and 2.5 million individuals are infected with HTLV- infected children4. (bvsalud.org)
Virus17
  • The manufacturer states that, for a test to be considered positive with this WB, antibody must be reactive with multiple virus-specific protein bands, i.e., p24, p31, and either gp41 or gp160 (Table 1). (cdc.gov)
  • ATLL develops in approximately 5% of HTLV-1 infected individuals who acquire the virus via vertical transmission [ 5 ]. (oncotarget.com)
  • Background: Hepatitis A Virus (HAV) is the most common cause of acute viral hepatitis worldwide, mostly in developing countries. (razavihospital.ir)
  • In this study, we compared patients who maintained virus suppression with patients experiencing viral relapse yet maintaining stable CD4+ lymphocytes while receiving antiretroviral therapy. (diseases-illness.com)
  • Men who have sex with men (MSM) are considered to be at significant risk for sexually transmitted infections (STI) and bloodborne viruses including viral hepatitis types B, C, and D (HBV, HCV, and HDV) and human T-cell leukemia virus types 1 and 2 (HTLV 1&2). (biomedcentral.com)
  • This study aimed to assess the seroprevalence and correlates of hepatitis B, C and D virus and anti-HTLV 1&2 antibodies among MSM, a high-risk population in Burkina Faso. (biomedcentral.com)
  • HTLV-1 causes a lifelong chronic illness in which virus-specific cell-mediated immune responses are a key determinant of viral weight. (iahrgrenoble2016.org)
  • Our data will be the first to point the fact that HTLV-1 mitochondrion-localizing proteins p13II comes with an important biological function through the early stage of virus infections in vivo. (arcticcirclescotland.com)
  • They found that several of the isolated antibodies were extremely potent against Ebola virus. (immunopaedia.org.za)
  • 2016. Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola virus outbreak. (immunopaedia.org.za)
  • If the viral DNA is expressed inside a host cell, the resulting RNA and protein molecules can be used to generate new virus particles. (meassociation.org.uk)
  • The group analyzed 589 prostate tumor samples for the presence of the virus and tested blood samples from 146 prostate cancer patients for antibodies to the virus and found no evidence of the virus or of antibodies to the virus. (meassociation.org.uk)
  • These include hepatitis C virus, human immunodeficiency virus (HIV), and human T-cell lymphotrophic virus (HTLV). (medscape.com)
  • Within 2 days after viral exposure, but before evidence of virus production in the monocytes, chemotactic activity was significantly impaired. (johnshopkins.edu)
  • The Challenges of Vaccine Growth in opposition to Betacoronaviruses: Antibody Dependent Enhancement and Sendai Virus as a Attainable Vaccine Vector To design an efficient and protected vaccine in opposition to betacoronaviruses, it's mandatory to make use of their evolutionarily conservative antigenic determinants that can elicit the mix of sturdy humoral and cell-mediated immune responses. (grdr.info)
  • Manufacturing of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparability of Platform Applied sciences for Viral Vector Manufacturing Modified Vaccinia Ankara (MVA) virus is a promising vector for vaccination in opposition to numerous difficult pathogens or the therapy of some varieties of cancers. (grdr.info)
  • Les examens ont été réalisés au Centre d'Infectiologie Charles Mérieux (CICM) de Bamako avec le dépistage du génome des virus responsables de la Dengue, de la fièvre de la Vallée du Rift, et du Zika à l'aide de la technique de la RT-PCR en temps réel. (bvsalud.org)
Protein5
  • The antibody is used to detect HTLV-I surface protein and its precursor in lysates prepared from virions or infected cells. (zeptometrix.com)
  • Using Western blotting and/or radioimmunoprecipitation analysis, the biosynthesis and metabolism of viral surface protein may be studied. (zeptometrix.com)
  • Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity. (zeptometrix.com)
  • Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)
  • Predicated on the lengthy amount of and the reduced percentage of people ASP9521 who develop ATL latency, transformation of contaminated lymphocytes is thought to be initiated through the induction of mobile genes and modifications in mobile activation and loss of life pathways by HTLV-1 protein (18, 35). (arcticcirclescotland.com)
Epitopes bound1
Indirect immunofluorescence1
  • It also reacts with HTLV-I infected cell lines using indirect immunofluorescence. (zeptometrix.com)
Serologic1
  • In addition, antibody-based serologic testing is a valuable epidemiologic tool to assess COVID-19 spread and potential immunity to SARS-CoV-2. (cdc.gov)
Prevalence2
  • This study shows that HBV, HCV and HTLV 1&2 prevalence among MSM in Burkina is high and suggests that comprehensive STI prevention and sexual health education services for this group are needed. (biomedcentral.com)
  • Brazilian populations have been performed in specific groups, restricted breastfeeding in mothers infected with HTLV-1 and such as blood donors or pregnant women, and the prevalence HTLV-2 (DAB/CAB23, 2009, BMoH). (bvsalud.org)
Viruses3
  • Moreover, recent research has shown that even viruses which were hitherto believed not to persist after an acute infectious episode are capable of long-term viral persistence. (investinme.org)
  • While there was very little information on BioClonetics at the time, investors now know that the company truly is using AI to develop additional monoclonal antibodies against common binding sites on deadly viruses such as HIV and SARS-CoV-2. (insiderfinancial.com)
  • The influenza viruses are the most common viral cause of pneumonia. (medscape.com)
Titers1
  • We evaluated antispike (anti-S) IgG antibody titers before and after another BNT162b2 dosage in people aged 60 years and old because this people is at risky WW298 of developing serious SARS-CoV-2 disease and was the first ever to receive authorization for the third dosage. (iahrgrenoble2016.org)
Specific8
  • By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific. (affbiotech.com)
  • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (cusabio.com)
  • Age-specific seroprevalence of anti-hepatitis a antibody among 1-30 years old population of savadkuh, mazandaran, iran with literature review. (razavihospital.ir)
  • Strategies Appearance antibodies and plasmids The pME18S p12ISL appearance plasmid continues to be described previously.19 p12I and its own mutants had been generated by polymerase chain reaction (PCR) or with the QuickChange Site-Directed Mutagenesis Kit (Stratagene, La Jolla, CA) using site-specific mutagenic oligonucleotides following manufacturer's instructions. (estme.org)
  • Altogether, the researchers were able to isolate 349 GP-specific antibodies from this donor. (immunopaedia.org.za)
  • Immunoprecipitation of the gp120 with a specific antibody reversed its effects on monocyte chemotaxis and HLA-DR expression. (johnshopkins.edu)
  • Consistent with this result, none of the sera analyzed from prostate cancer patients contained XMRV-specific antibodies. (biomedcentral.com)
  • Furthermore, none of the sera contained antibodies specific for the XMRV Env or Gag proteins. (biomedcentral.com)
Bound2
  • They found that the donor antibodies bound to four distinct epitopes on the GP. (immunopaedia.org.za)
  • This includes: antibody development, viral antigen characterisation, and the development of a membrane bound version of the molecular clamp. (edu.au)
Patients with viral1
  • The relationship between viral and immunologic factors in patients with viral relapse on antiretroviral therapy is complex and incompletely understood. (diseases-illness.com)
Infectious2
  • These data suggest that ACH, like wild-type HTLV-1, infects and transforms primary CD4 + T lymphocytes and is infectious in vivo. (wustl.edu)
  • Unlike HIV-1, extracellular HTLV-1 particles are poorly infectious and viral transmission typically occurs via direct intercellular interactions across a virological synapse. (longdom.org)
Infections5
  • Plasma calprotectin can be used, either solely or in combination with other inflammation markers, to distinguish between bacterial and viral infections [2]. (geticadiagnostics.com)
  • Only 0.1%-3% of HTLV‐1 infections result in T cell leukemia. (diseases-illness.com)
  • The root mechanisms where HTLV-1 establishes a continual infections and transforms lymphocytes continues HDAC9 to be extensively looked into but incompletely grasped. (arcticcirclescotland.com)
  • Latest research reveal a substantial function for HTLV-1 accessories proteins in the entire lifestyle routine of HTLV-1, particularly through the early stage from the viral infections of lymphocytes (evaluated in sources 12, 28, and 32). (arcticcirclescotland.com)
  • In this scholarly study, we motivated the functional need for HTLV-1 p13II in the establishment of infections within a rabbit model. (arcticcirclescotland.com)
Experimentally1
  • Optimal dilution of antibody must be determined experimentally by the investigator. (zeptometrix.com)
Detectable1
  • False-negative test results are observed among persons who have recently become infected with HIV and who have not yet developed detectable antibody (6). (cdc.gov)
Antigenic1
  • Western blot (immunoblot) analysis revealed sustained seroconversion of rabbits to positivity for antibodies against all major vital antigenic determinants. (wustl.edu)
Infectivity1
  • These scholarly studies, however, still left open up the relevant issue from the function of p13IWe by itself in viral infectivity in vivo. (arcticcirclescotland.com)
Proteins2
  • So that they can reconcile the disparate features of the viral proteins apparently, we discovered that the p12I proteins undergoes complicated posttranslational modifications including proteolytic cleavage between amino acidity positions 9 and 10 accompanied by another cleavage between your proteins 29 and 30. (estme.org)
  • The initial proteolytic cleavage gets rid of a noncanonical ER retention/retrieval sign on the amino terminus of p12I and permits further trafficking of the viral proteins towards the Golgi equipment as well as the lipid rafts. (estme.org)
Plasma1
  • Since that time, millions of HIV antibody tests have been performed in laboratories of blood and plasma collection centers, in counseling and testing centers, and in clinical facilities as well as for purposes such as screening active duty military personnel and applicants for military service. (cdc.gov)
Vaccines2
  • Antibodies are the correlate of protection in most of the available, effective anti-viral vaccines. (immunopaedia.org.za)
  • Vero cell upstream bioprocess improvement for the manufacturing of viral vectors and vaccines The Vero cell line is taken into account essentially the most used steady cell line for the manufacturing of viral vectors and vaccines. (grdr.info)
Gene3
  • The epitope is located between amino acids 190-209 of the viral env gene. (zeptometrix.com)
  • Other than directly administering the cytokines, another method involves introducing the gene that codes for the cytokine into a viral vector. (oncolink.org)
  • Gene-Modifying Applied sciences Paired With Viral Vectors for Translational Analysis Into Neurodegenerative Ailments Ailments of the central nervous system (CNS) have traditionally been among the many most troublesome to deal with utilizing typical pharmacological approaches. (grdr.info)
Confirmatory1
  • HTLV-1: a health problem in Brazil can substantially reduce costs associated with confirmatory derived from the blood or produced locally10. (bvsalud.org)
Presence1
  • Presence of anti-HTLV 1&2 antibodies was associated with history of genital or anal lesions and injection drug use. (biomedcentral.com)
Epidemiology1
  • In Sub-Saharan Africa, very few data exist on hepatitis B, C, and D as well as on HTLV 1&2 epidemiology among MSM. (biomedcentral.com)
Mice1
  • Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)