Viability malignant b cells. Medical search. Frequent questions

The presented study investigates the effect of anti-epileptic drugs on the viability and proliferation of synovial fibroblasts in vitro . (aaem.pl)
In Vitro Cell Dev Biol. (aaem.pl)
These phytochemicals have been tried against cancer viability at both in vitro and in vivo levels. (researchsquare.com)
Moreover, loss-of-function studies reveal that restoration of linc01023 restrains glioma cell proliferation, migration and invasion by regulating IGF1R/AKT pathway in vitro and in vivo. (jcancer.org)
Moreover, linc01023 depletion inhibited glioma cell proliferation, migration and invasion by regulating IGF1R/AKT pathway in vitro and in vivo. (jcancer.org)
As a result, the proliferation and in vitro AMH and oestrogen production of the cells were decreased at post-exposure 24h. (bmj.com)
Calretinin is essential for mesothelioma cell growth/survival in vitro: A potential new target for malignant mesothelioma therapy? (unifr.ch)
Down-regulation of CR via lentiviral-mediated shRNA significantly decreased the viability and proliferation of mesothelioma cells in vitro . (unifr.ch)
The results in vitro demonstrated an association between COX-2 activity and intracellular MDA levels, and a correlation in malignant cells between MDA concentration and M1G adduct levels. (le.ac.uk)
cell proliferation in vitro. (researchtoactionforum.org)
In FANCD vitro cell proliferation assay Cell proliferation was determined using the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium (MTT) dye reduction technique [20]. (researchtoactionforum.org)
In vitro PDT activity was studied using human mesothelioma cell lines (biphasic MSTO-211H cells and epithelioid NCI-H28 cells) alongside a non-malignant mesothelial cell line (MET-5A). (shu.ac.uk)
Malignant transformation in vitro was determined by using the anchorage- independent growth assay on soft agar. (scirp.org)
Sabinsa's Curcumin C3 Complex ® in combination with BioPerine ® (CBP) has an inhibitory effect on malignant mesothelioma cells, according to an in vitro and in vivo study published in Journal of Experimental and Clinical Cancer Research . (leangard.com)
In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
Study conclusion: "The results do not support previous reports of beneficial effects of spiritual healing on malignant cell growth in vitro. (ebm-first.com)
In vitro studies based on MCF-7 cell proliferation and induction of vitellogenin in primary culture of rainbow trout hepatocytes. (cdc.gov)

Effects of valproic acid (VPA) and levetiracetam (LEV) on proliferation, apoptosis and hormone secretion of the human choriocarcinoma BeWo cell line. (aaem.pl)
Therefore, in this review, we avoid a lengthy repetition of the description of autophagy and cell death processes and focus on the death-promoting roles of autophagy and the intertwined connection between autophagy and apoptosis. (nature.com)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
Introduction/Background We aimed to compare gemcitabine vs. cisplatin in terms of DNA damage response, viability/apoptosis of malignant granulosa cells. (bmj.com)
Cell viability, proliferation, DNA damage response and apoptosis were investigated using immunofluorescence staining and immunoblotting. (bmj.com)
Results Gemcitabine treatment caused DNA damage, cellular stress, inhibited proliferation and activated cell cycle check-point sensors and induced apoptosis as shown by increased expression of g-histone H2AX, p-JNK, Chk-1/Chk-2, cleaved forms of PARP and caspase-3 in the asynchronous cells in a dose dependent manner. (bmj.com)
Depletion of CR led these cancer cell lines to enter apoptosis within 72h post-infection via strong activation of the intrinsic caspase 9-dependent pathway. (unifr.ch)
Down-regulation of CR in immortalized mesothelial cells LP9/TERT-1 strongly blocked proliferation and caused a G₁ block without decreasing viability or activating apoptosis pathways. (unifr.ch)
Our study resulted in sequences of events marked by apoptosis, such as loss of cell viability, morphology changes that were evaluated by MTT assay and invert-microscope, respectively. (phcog.com)
Black seed oil induces apoptosis, which means that it helps the body to systematically eliminate old cells, unneeded cells, and unhealthy cells (such as cancer cells) without releasing toxins into the body. (corespirit.com)
The migration was investigated by the transwell, and the apoptosis and cell cycle were assayed by the flow cytometry. (biomedcentral.com)
Onset of apoptosis of cancer cells in drug susceptible and resistant ones was also determined occur by activation of intrinsic pathway through altered ratio of Bax/BCl2 proteins. (leangard.com)
Efficacy of CBP was further demonstrated in a xenograft model of MM. Low cancer cell proliferation (as judged by Ki-67 levels), higher TUNEL score indicating enhanced apoptosis and reduced angiogenesis in tumor cells were noted in the animal graft study. (leangard.com)
Capsaicin (CAP), the active ingredient of hot chilli peppers, is one of a number of related endogenous and plant-derived compounds (broadly termed 'vanilloid-like agents') that have been shown to induce apoptosis and inhibit cell proliferation in some cancer cells, through both TRPV1-dependent and -independent mechanisms. (edu.au)

Georgescu HI, Mendelow D, Evans CH. HIG-82: an established cell line from rabbit periarticular soft tissue, which retains the "activatable" phenotype. (aaem.pl)
According to recent data, Ras-transformed cells require autophagy to survive and maintain malignant phenotype [ 5 - 10 ]. (aging-us.com)
Knockdown of EDG2 reversed the EMT phenotype in HCC cells. (liveconscience.com)
Desk 2 Univariate and multivariate studies of prognostic elements in HCC sufferers after liver organ resection EDG2 expanded the cell viability, growth, migration and breach of Huh7 cells through generating EMT phenotype To address the function of EDG2 on the pathogenesis of HCC, we constructed Huh7 cell model with improved EDG2 reflection via transfecting EDG2 showing plasmid. (liveconscience.com)
In this study, our aims were to explore the role of FASN in regulating the "HER2-PI3K/Akt axis" activity and malignant phenotype of colorectal cancer. (biomedcentral.com)
A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
Overexpression of the epidermal growth factor receptor (EGFR) is associated with the malignant phenotype in many cancers including ovarian cancer, which leads to increased cell proliferation and survival. (jcancer.org)
Max Planck Institute (MPI) cells, a non-transformed mouse line that has an AM phenotype and genotype, were treated with mass-, surface area- (s.a.), and particle number- (p.n.) equivalent concentrations of respirable asbestiform and non-asbestiform riebeckite/tremolite EMP for 24h. (cdc.gov)

The cytotoxic effects of constructed idarubicin-Z HER2 affibody conjugate on the SK-BR-3, SK-OV-3, and MCF-7 cells with various levels of HER2 expression were evaluated by MTT assay after 48 hours of incubation time. (iranpath.org)

miR-221, an oncogenic microRNA, can promote cell proliferation and is highly expressed in various types of tumors. (elsevierpure.com)
Our aims were to elucidate whether T cells can induce galectin-9 secretion in human cancer cells derived from solid malignant tumors and whether this soluble form displays higher systemic immunosuppressive activity compared with the cell surface-based protein. (bmj.com)
Results We discovered that T lymphocytes induce galectin-9 secretion in various types of human cancer cells derived from solid malignant tumors. (bmj.com)
Previous studies had confirmed that Lasp1 was significantly overexpressed in multiple malignant tumors including non-small-cell lung cancer (NSCLC) and enhanced tumor proliferation, invasion and metastasis [ 7 - 13 ]. (oncotarget.com)
They have complementary mechanisms to hinder the carcinogenic process by searching free radicals, inhibiting survival and multiplication of malignant cells, as well as reducing invasiveness and angiogenesis of tumors. (researchsquare.com)
Thus, type I (low-grade serous G1, low-grade endometrioid G1/G2, mucinous or clear cell) tumors are associated with corresponding benign ovarian cystic neoplasms, often developing through an intermediate borderline step and have a better prognosis. (spandidos-publications.com)
Glioma, the vast majority of intracranial malignant tumors, is characterized by rapid proliferation and aggressive invasion [ 1 ]. (jcancer.org)
HCC is the leading type of the malignant liver organ tumors with the unsatisfied treatment. (liveconscience.com)
In vivo data showed that treatment with S-1 significantly reduced thoracic tumors and pleural effusion produced by Y-MESO-14 cells. (researchtoactionforum.org)

Cellular viability assay and western blot analysis were used to measure cell viability and expression levels of proteins, respectively. (jcancer.org)

However, solid tumor cells at rest are known to secrete either very low amounts of galectin-9 or, in most of the cases, do not secrete it at all. (bmj.com)
Lasp1 (LIM and SH3 domain protein 1) promotes tumor proliferation and invasion in multiple cancer entities including non-small cell lung cancer (NSCLC). (oncotarget.com)
We studied whether cellular senescence induced by HDAC inhibitor sodium butyrate in E1a+cHa-Ras -transformed rat embryo fibroblasts (ERas) and A549 human Ki-Ras mutated lung adenocarcinoma cells would enhance the tumor suppressor effect of MEK/ERK inhibition. (aging-us.com)
This can provide Ras-expressing tumor cells additional selective advantages under stress or damage. (aging-us.com)
Isolated EOC tumor cells obtained at surgery were investigated for their sensitivity to seven standard cytotoxic drugs and nine TKIs using a short‑term fluorescent microculture cytotoxicity assay (FMCA). (spandidos-publications.com)
The clinically observed relative drug resistance of EOC type I, as well as in patients previously treated, is at least partly due to mechanisms in the tumor cells. (spandidos-publications.com)
However, emerging evidence suggests that autophagy is a primary mechanism of cell death (autophagic cell death, ACD) and implicates ACD in several aspects of mammalian physiology, including tumor suppression and psychological disorders. (nature.com)
Although the efficacy of adoptive transfer of tumor infiltrating lymphocytes (TILs) was examined over several decades, genetically engineered T cells expressing chimeric antigen receptors (CARs) rapidly replaced the application of TILs due to their high specificity, non-MHC-restricted recognition of tumor antigen, superior potency, and improved in vivo persistency [ 9 , 13 , 14 ]. (biomedcentral.com)
Malignant pleural mesothelioma (MPM) is an aggressive tumor linked to environmental/occupational exposure to asbestos, characterized by the presence of significant areas of hypoxia. (units.it)
MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. (up.pt)
Even though the idea of an immune system actively searching for and disposing of potential mutated tumor cells is over a century old, only recent developments in various fields such as mass spectrometry, immuno-checkpoint blockade strategies and in silico modelling have enabled the realization of the full potential of recruiting immune system to fight cancer and the possibilities of personalized therapies. (helsinki.fi)
This master thesis is a part of a project aiming to set up immunoaffinity-purification/MS based method in order to analyse the ligandome and determine T-cell recognized cancer associated antigens from tumor cells. (helsinki.fi)
Characterizing tumor cell lines. (helsinki.fi)
Conclusion: Phosphorylation of Tm1 at Ser283 contributes to its anti-tumor properties, and this effect results mainly from an increase in cell adhesion associated with a decrease in their migratory and invasive potentials. (scirp.org)
Long non-coding RNAs (lncRNAs) have moved into the focus of cancer research as good candidates for tumor biomarkers and as regulators of various neoplastic cell properties. (biomedcentral.com)
Tanshinone II A is an effective component extracted from Salvia miltiorrhiza and the roles of Tanshinone IIA in regulating the stemness of tumor cells remain unclear. (biomedcentral.com)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)

Stander M, Dichgans J, Weller M. Anticonvulsant drugs fail to modulate chemotherapy-induced cytotoxicity and growth inhibition of human malignant glioma cells. (aaem.pl)
It plays an important role in brain development and is crucial for the viability of malignant glioma cells. (nih.gov)

Macroautophagy (hereafter referred to as autophagy) plays an important role in cancer cells survival upon various conditions of intra- and extracellular stress. (aging-us.com)
Sensitization of cancer cells to conventional drugs using multi-target agents that block survival and oncogenic pathways, alone or in combination, is an emerging strategy to overcome drug resistance. (researchsquare.com)
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. (nih.gov)
PTEN insufficiency promotes the PI3K/AKT path, which is a vital signal for the survival of cancer cells. (nature.com)
Previous studies have demonstrated that IGF-1R activates the phosphoinositide 3- kinase (PI3K)/AKT signaling pathways and promotes glioblastoma cell proliferation and survival by ligand binding [ 10 ]. (jcancer.org)
Autophagy is generally considered as a cell survival/protection mechanism because it removes toxic or obsolete proteins and organelles and recycles the degradation products for use as sources for energy and metabolites in anabolic pathways 3 . (nature.com)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)
Moreover, treatment with S-1 prolonged the survival of Y-MESO-14 cell-bearing SCID mice. (researchtoactionforum.org)
It also controls the Akt pathway, which means it controls the process that manages cell survival for both normal and cancer cells. (corespirit.com)
Malignant pleural mesothelioma (MPM) is an aggressive inflammatory cancer with a poor survival rate. (frontiersin.org)

Our study indicates that exosomal miR-221 derived from HQ-transformed malignant human bronchial epithelial cells is involved in the proliferation of recipient cells. (elsevierpure.com)
We investigated the potential of the ethanolic extract of saffron to induce antiproliferative and cytotoxic effects in cultured carcinomic human alveolar basal epithelial cells in comparison with non-malignant (L929) cells. (phcog.com)
In this study, we aimed to assess the neoplastic-like transformation potential of two forms of CNC derived from wood (powder and gel) in human pulmonary epithelial cells (BEAS-2B) in comparison to fibrous tremolite (TF), known to induce lung cancer. (cdc.gov)

Our investigation revealed that expression levels of miR-221 were significantly increased in HQ-transformed malignant cells relative to normal controls. (elsevierpure.com)
Idarubicin showed a potent and dose-dependent cytotoxic effect against all treated cell lines while the SK-OV-3 cells were significantly more sensitive. (iranpath.org)
In the cells synchronized at S phase gemcitabine significantly inhibited DNA synthesis and blocked their proliferation. (bmj.com)
As proven in Number ?Number2A,2A, both qRT-PCR and western immunoblotting showed that there was significantly more EDG2 appearance in Huh7 EDG2 cells than Huh7 Vector cells. (liveconscience.com)
The Se-enriched exopolysaccharide fraction significantly enhanced cell viability when incubated with normal (human umbilical vein endothelial cells, HUVEC) cells (but this effect was absent for malignant human cervical HeLa cells) and this fraction also protected the cells from oxidative stress conditions. (preprints.org)
Notably, a TG2-selective irreversible inhibitor that reacts with the intracellular active form of TG2, but not a non-cell-permeable inhibitor, significantly compromised cell viability in MPM spheroids. (units.it)
However, the extract at different concentrations could not significantly decrease the cell viability in L929 cells. (phcog.com)

Analysis of TCGA pan-cancer data revealed significant overexpression of CASC9 across different other entities including bladder, liver, lung and stomach cancers and especially in squamous cell carcinoma (SCC) of the lung. (biomedcentral.com)
This thesis investigated the expression and function of TRPV1 in haematological malignancies, using both blood cancer cell lines and blood samples obtained from patients with different blood cancers. (edu.au)
1) study the effect of TRPV1 agonists and antagonists on the viability of THP-1, U266B1 and U937 haematological malignant cell lines, 2) validate and optimise Western blotting and flow cytometry protocols to detect TRPV1 expression in leukocytes, 3) investigate TRPV1 expression in THP-1, U266B1 and U937 cells, and 4) compare TRPV1 expression in leukocytes obtained from patients with blood cancers to normal subjects. (edu.au)
Akt2-dependent signaling appears to play an important role in EGFR-mediated cellular viability in ovarian cancer and targeting specific Akt isoform may provide a potential therapeutic approach for EGFR-expressing ovarian cancers. (jcancer.org)
As in other head and neck cancer sites, more than 95% of hypopharyngeal malignancies arise from the epithelium of the mucosa and, therefore, are squamous cell cancers. (medscape.com)

Western blot analysis, ELISA, quantitative reverse transcriptase-PCR, on-cell Western and other measurement techniques were used to conduct the study. (bmj.com)
Caco-2 cells were transfected with either the FASN specific RNAi plasmid or the negative control RNAi plasmid, followed by the RT-qPCR and western blot to examine the expression of FASN, HER2, PI3K and Akt. (biomedcentral.com)

Methods A wide range of human cancer cell lines derived from solid tumours, keratinocytes and primary embryonic cells were employed, together with helper and cytotoxic T cell lines and human as well as mouse primary T cells. (bmj.com)
In the current study, we assessed the cytotoxic effects of idarubicin-Z HER2 affibody conjugate on the positive-HER2 cancer cell lines. (iranpath.org)
The Z HER2 -affibody conjugate of idarubicin has a more specific cytotoxic effect compared with idarubicin alone against HER2-overexpressing ovarian cancerous cells. (iranpath.org)
Idarubicin has a potent and dose-dependent cytotoxic effect against breast and ovarian cancerous cells. (iranpath.org)
The Z HER2 -affibody conjugate of idarubicin has a more potent cytotoxic effect than idarubicin alone against HER2-overexpressing malignant cells. (iranpath.org)
These therapeutic methods, including but not limited to oncolytic virus therapies, T-cell therapies and cancer vaccines, are based on the body's ability to recognize mutated antigen peptides presented on the cell surface by MCH-receptors (also known as HLA-receptors in humans) and the disposal of the malignant cells by cytotoxic T-cells. (helsinki.fi)
At the equal mass, asbestiform EMP were more cytotoxic, however EMP of both habits induced similar LDH leakage and decrease in viability at s.a. and p.n. equivalent doses. (cdc.gov)

ROCK kinases induce actomyosin-based contractility and phosphorylate TAU and MAP2 involved in regulating myosins and other actin-binding proteins in order to assist in cell migration and detachment. (wikipedia.org)
To detect the effects of miR-183 on the migration of BC cell lines, wound healing was used along with a Trans-well migration assay. (nature.com)
MiR-183 can exert an oncogenic effect by promoting cell viability, migration, and cell cycle progression. (nature.com)
In the present study, the effect of miR-183 on cell viability, cell cycle progression, and migration was investigated, and the interaction between miR-183 and PTEN in BC cells was examined. (nature.com)
MTT, Transwell, scratch, western blotting and reverse transcription‑quantitative PCR assays were performed to examine how curcumol inhibited CRC cell viability, invasion and migration, and to detect the role of miR‑30a‑5p and curcumol in the invasion and Hippo signaling pathways of CRC cells. (spandidos-publications.com)
The results demonstrated that miR‑30a‑5p downregulation was accompanied by the inactivation of the Hippo signaling pathway, which was demonstrated to promote CRC cell viability, invasion and migration. (spandidos-publications.com)
Curcumol treatment was identified to increase miR‑30a‑5p expression and to activate the Hippo signaling pathway, which in turn inhibited the invasion and migration of CRC cells. (spandidos-publications.com)
Overexpression of miR‑30a‑5p enhanced the effects of curcumol on cell invasion and migration, and the Hippo signaling pathway in CRC cells. (spandidos-publications.com)
It has been reported that numerous miRNAs, including the miR-30 family, serve important roles in the regulation of cancer cell migration and invasion ( 5 - 7 ). (spandidos-publications.com)
It has been reported that miR-30a-5p expression is decreased in primary gallbladder cancer (GBC) lesions, and inhibition of its expression in GBC cells promoted migration and invasion ( 8 ). (spandidos-publications.com)
Cell migration ability melanoma A375 was monitored by using cell scratch assay. (biomedcentral.com)
Tan II A reduced CXCL12-induced A375 cell invasive ability and migration in a dose dependent manner. (biomedcentral.com)
Furthermore, EDG2 over-expression in HCC cells brought the Rabbit Polyclonal to CHSY1 usual EMT features including up-regulation of Vimentin, N-cadherin and Fibronectin, reductions of E-cadherin, and enhanced cell breach and migration sizes. (liveconscience.com)
These data indicated that EDG2 mediated positively HCC cell migration and attack. (liveconscience.com)
The migration was also impaired in FASN-silenced cells. (biomedcentral.com)
And the influence of FASN on proliferation and migration of colorectal cancer cells was also explored. (biomedcentral.com)
Cell migration was evaluated by using the wound-healing assay and by measuring transendothelial migration of cancer cells. (scirp.org)
Moreover, the migration of MDA-MB231/S283E/Tm1 cells in a wound closure assay is reduced compared to parental cells or those expressing the non-phosphorylatable form of Tm1 (S283A). (scirp.org)
Similarly, the transendothelial migration of MDA-MB231/S283E/Tm1 cells is also reduced as compared to the other cell lines. (scirp.org)
Simoneau, B., Houle, F. and Huot, J. (2012) Regulation of Endothelial Permeability and Transendothelial Migration of Cancer Cells by Tropomyosin-1 Phosphorylation. (scirp.org)
The researchers found that CBP worked well in synergistically impairing the cancer cell viability, cellular self-renewal ability, cell proliferation rate, and cell migration as assessed by wound-healing assay. (leangard.com)
Effects of lncRNA overexpression or downregulation on cell proliferation, clonogenicity, migration and chemosensitivity were studied in HNSCC cell lines. (biomedcentral.com)
Unexpectedly, up- or downregulation of CASC9 expression in HNSCC cell lines with low or high CASC9 expression, respectively, did not result in significant changes of cell viability, clonogenicity, migration or chemosensitivity. (biomedcentral.com)

Dose-dependent response of CKAP5 knockdown across various cancer cell lines. (medicalxpress.com)
Cells that did not show any effect of CKAP5 knockdown are highlighted with red boxes. (medicalxpress.com)
Notably, miR-125b overexpression enhanced the stemness of breast cancer cells, and miR-125b overexpression or STARD13 knockdown impaired the inhibitory effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
Knockdown of Akt2 blocked EGF-induced OVCAR-3 cell viability whereas knockdown for Akt1 and Erk1/2 had no significant effect. (jcancer.org)

Methodology Malignant granulosa tumour cell lines (COV434 and KGN) were used for the experiments. (bmj.com)

Among several markers that have been defined for EOC, DNA damage and repair system factors, molecules involved in the G1 cell cycle, PI3K pathway, MAPK pathway, and also growth factors attracted more attention for targeted therapy (3). (iranpath.org)
We concluded that Tan II A reduced A375 cells proliferation by activation of autophagy production, blocked PI3K- Akt - mTOR - p70S6K1 signaling pathway, increased autophagic related gene beclin-1, LC3-II protein expressions and induced autophagocytosis. (biomedcentral.com)
CR protects against asbestos-induced acute cytotoxicity mediated by the AKT/PI3K pathway and furthermore, SV40 early region genes are able to up-regulate CR in mesothelial cells. (unifr.ch)
A transcriptome analysis of HER2 in breast cancer cells has revealed a molecular connection between FASN and HER2 through the PI3K/Akt pathway [ 10 ]. (biomedcentral.com)
PI3K activity was thought to be the mediator of the HER2 control on FASN expression because LY294002, a known PI3K inhibitor, abrogated HER2 induced FASN protein production in the HER2 overexpressing normal mammary epithelial and breast cancer cells. (biomedcentral.com)
Hence, in this study, we analyzed the potential link between FASN and the activity of "HER2-PI3K/Akt axis" in colorectal cancer cells. (biomedcentral.com)
24 h) stimulates the malignant phenotypes of PCa cells and activates PI3K/AKT signalling pathway in PCa cells [6] . (medchemexpress.com)
On the other hand, the EGFR and PI3K inhibitors decreased EGF-induced cellular viability, indicating the involvement of Akt signaling. (jcancer.org)

RhoA specifically is regarded as a prominent regulatory factor in other functions such as the regulation of cytoskeletal dynamics, transcription, cell cycle progression and cell transformation. (wikipedia.org)
Moreover, flowcytometry was applied to analyze the effects of miR-183 on the cell cycle progression. (nature.com)
However, the detailed regulatory mechanism of curcumol in the malignant progression of CRC remains unknown. (spandidos-publications.com)
Exposure of the cells to gemcitabine at G2/M transition abolished the progression of mitosis, caused mitotic arrest and failure to exit mitosis as evidenced by the inhibition of Cyclin B degradation and absence of de-phosphorylation of cdc-2 at Tyr 15 residue. (bmj.com)
Cell cycle progression was also affected. (frontiersin.org)

The successful strategy based on the activation of cell death in Ras-expressing cells is associated with the suppression of kinases involved in Ras pathway. (aging-us.com)
Senescent ERas cells do not develop cytoprotective autophagy upon inhibition of MEK/ERK pathway due to spatial dissociation of lysosomes and autophagosomes in the senescent cells. (aging-us.com)
Our data show that suppression of MEK/ERK pathway in senescent cells provides a new strategy for elimination of Ras-expressing cells. (aging-us.com)
Despite a large number of promising inhibitors for Ras/Raf/MEK/ERK pathway, recent works show that cancer cells often develop an autophagy-dependent resistance to inhibitors of Ras pathway [ 16 ]. (aging-us.com)
High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)
We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. (biomedcentral.com)
The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. (up.pt)
This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. (up.pt)
Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment. (up.pt)
The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)

RhoA, and the other Rho GTPases, are part of a larger family of related proteins known as the Ras superfamily, a family of proteins involved in the regulation and timing of cell division. (wikipedia.org)
Background Galectin-9 is a member of the family of lectin proteins and crucially regulates human immune responses, particularly because of its ability to suppress the anticancer activities of T lymphocytes and natural killer cells. (bmj.com)
Secreted galectin-9 pre-opsonised T cells and, following interaction with other immune checkpoint proteins, their activity was completely attenuated. (bmj.com)
As an example, we studied the cooperation of galectin-9 and V-domain Ig-containing suppressor of T cell activation (VISTA) proteins in human cancer cells. (bmj.com)
G: Levels of epithelial-mesenchymal transition-related proteins (E-cadherin and N-cadherin) in AGS and HGC-27 cells determined using western blotting. (wjgnet.com)
The host cell exerts its self-clearing of toxic substances such as damaged proteins and organelles through autophagy processes [ 7 ]. (biomedcentral.com)
Traditional western blotting for dedication of DPD and TP proteins level The entire cell components had been ready with Ripa lysis stream (Thermo Fisher Scientific Inc., Rockford, IL), and proteins concentrations had been tested using Bradfords technique. (researchtoactionforum.org)
Twenty g of total cell remove proteins had been electrophoresed on 4C10% NuPAGE? (researchtoactionforum.org)
Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)

RhoA is prevalent in regulating cell shape, polarity and locomotion via actin polymerization, actomyosin contractility, cell adhesion, and microtubule dynamics. (wikipedia.org)
In addition, RhoA is believed to act primarily at the rear (uropod) of migrating cells to promote detachment, similar to the attachment and detachment process found in the focal adhesion mechanism. (wikipedia.org)
S. aureus bacterial culture showed good cell adhesion and spreading inwards into the chitosan nanofibrous membrane. (ru.ac.za)
Adhesion assays were performed at various periods of time on cells grown on plastic. (scirp.org)
Results: We found that cells expressing the phosphomimetic form of Tm1 S283E/Tm1 are characterized by an increased adhesion to the substratum. (scirp.org)

Few diagnostic and prognostic biomarkers are available for head-and-neck squamous cell carcinoma (HNSCC). (biomedcentral.com)
SquamouS cell carcinoma for the viability of the tumour cel s once skin lesions are also mutated in the ultraviolet they have become malignant (Rol ison (UV) radiation-induced skin lesions of A large number of HPV types have et al. (who.int)

Carbamazepine inhibited proliferation of human fibroblasts and viability of HIG-82 with IC 50 values of 86 µM and 82 µM, respectively. (aaem.pl)
The results of tests on the proliferation of human peripheral blood mononuclear cells suggested a selective immunosuppressive activity, like previously tested Se-polyglucans isolated from L. edodes mycelium. (preprints.org)

We hope to be able to non‐invasively discriminate between benign and malignant skin lesions and to define tumour margins, which today can only be done by surgical excision and histological analysis. (lu.se)

Many innovative methods have been developed with genetic modification of cells being the most advanced cell surface engineering technique. (biomedcentral.com)
In silico methods were successfully used to characterize previously identified HLA-restricted peptides and one previously identified immunogenic T-cell epitope. (helsinki.fi)
Methods: MDA MB231 breast cancer cells stably overexpressing wild type form of Tm1 or Tm1 mutants (S283A and S283E) were generated. (scirp.org)

Reports of cancer from expo- sure to malignant cells are quite rare. (cdc.gov)
Although the incidence of cutaneous injuries resulting from sharps contaminated with viable cancer cells is unknown, it probably represents only a small fraction of the cutaneous injuries incurred by potentially exposed workers. (cdc.gov)
Metformin (MTF) has been reported to target NLK (Nemo-like kinase) to inhibit non-small lung cancer cells. (cancerindex.org)
Enhancement of HER-2 degradation by carbamazepine in breast cancer SKBR-3 cell line]. (aaem.pl)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Cancer cells exploit a variety of protective mechanisms that allow them to acquire selective advantage and proliferate under unfavorable conditions. (aging-us.com)
One of the mechanisms of high basal autophagy activity in cancer Ras-expressing cells might be associated with high PP2A phosphatase activity targeting directly ULK1-Ser757 that could explain apparent contradiction: maintenance of high mTORC1 functions and high autophagic activity simultaneously [ 22 ]. (aging-us.com)
Cancer is characterized as the uncontrolled multiplication and spread of the body's particular cells. (researchsquare.com)
Through qRT-PCR analysis, miR-183 and PTEN mRNA levels in breast cancer cell lines were measured. (nature.com)
Thin skin and hair loss are effects of radia- tion therapy for mast cell cancer. (veterinarypracticenews.ca)
Cancer cells often maintain high basal level of ROS and are vulnerable to any further increase in ROS levels beyond a certain protective threshold. (degruyter.com)
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)
This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)
Cancer cells also enhance glucose-uptake to fuel these enhanced metabolic activities [ 2 ]. (degruyter.com)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
This is because cancer cells maintain high basal level of ROS, thus they are vulnerable to any further increase in ROS (Fig. 1 ). (degruyter.com)
ROS-modulation in normal vs. cancer cell. (degruyter.com)
There is not cured for MM. Tan II A has been reported to reduce cancer cell proliferation. (biomedcentral.com)
In esophageal cancer, radiation therapy was found inducing autophagy in cancer cells, promoting cancer cell proliferation and causing treatment resistance [ 10 ]. (biomedcentral.com)
Other reports suggested that autophage suppressed cancer development in early stage and promoted cancer cell proliferation in later stage [ 4 ]. (biomedcentral.com)
For anticancer activity evaluation the treated cells with VNPs@Salvia officinalis were assessed by MTT assay for 48h about the cytotoxicity and anti-colorectal cancer properties on normal (HUVEC) and colorectal cancer cell lines i.e. (authorea.com)
The unit effectively reduced the viability of triple-negative breast cancer up to 80% without thermal damage, providing a starting point for future clinical trials. (mdpi.com)
Cell-based therapy has expanded its influence in cancer immunotherapy, regenerative medicine, and tissue engineering. (biomedcentral.com)
Unfortunately, these breakthrough discoveries in both regenerative medicine and cancer immunotherapy using cells as therapeutic reagents soon faced a common problem: the inability to control cellular functions to maximize the therapeutic benefits. (biomedcentral.com)
The developmental story of cell-based cancer immunotherapy is not so different from MSCs in regenerative medicine. (biomedcentral.com)
Title: Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles Abstract: Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. (up.pt)
We also showed that even higher concentrations of saffron is safe for L929, but the extract exerts pro-apoptotic effects in a lung cancer-derived cell line and could be considered as a potential chemotherapeutic agent in lung cancer. (phcog.com)
Black cumin seed oil stops cancer cell activity and can even kill some kinds of cancer cells. (corespirit.com)
Preliminary MTS assay is used to determine linear range and optimal time frame for the PBMC/cancer cell co-culture killing assay. (helsinki.fi)
Another challenge is the cell entry of oncolytic adenovirus, which is mainly mediated by the Coxsackie-Adenovirus receptor and this receptor is downregulated in various cancer cells. (helsinki.fi)
In order to overcome these limitations, this study explored the possibility to use cancer cell derived extracellular vesicles (EVs) as drug delivery system for oncolytic adenovirus. (helsinki.fi)
However, in cancer cells and pre-neoplastic lesions, FASN expression has been frequently found to be upregulated. (biomedcentral.com)
It has been shown that the growth factors, hormones and activation of their receptors increased FASN transcription in cancer cells. (biomedcentral.com)
In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
The researchers screened 20 solid cancer cell lines relative to gene silencing to identify a highly responsive chemo-resistant ovarian cancer cell line that underwent significant depletion in mitotic spindle-dynamics for effective experimental cancer treatment. (medicalxpress.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)
Malignant Mesothelioma (MM) is an aggressive form cancer primarily associated with exposure to asbestos fibers. (leangard.com)
38:Article no. 360 ), explored the combined use of CBP in drug susceptible and drug-resistant MM cancer cell-types. (leangard.com)
Overall, these results indicate that cNPs have anti-proliferative effects on bone cancer cells and cancer cells that metastasize to the bone. (emerginginvestigators.org)
Additionally, Tanshinone IIA enhanced adriamycin sensitivity and attenuated adriamycin resistance of breast cancer cells. (biomedcentral.com)
The study has previously revealed that miR-125b faciliates cell migratory ability breast cancer via targeting STARD13 (StAR-related lipid transfer protein 13) [ 20 ]. (biomedcentral.com)
Here we investigated the differential effect of Akt on the EGF-induced cell viability in a panel of ovarian cancer cell lines. (jcancer.org)
Cellular viability increased in OVCAR-3 ovarian cancer cells exposed to EGF, but little to no difference was observed in the 5 other ovarian cancer cells including SKOV-3 cells despite of the expression of EGFR. (jcancer.org)
Furthermore, we observed a different expression of Akt isoforms: Akt1 was constitutively expressed in all tested ovarian cancer cells, while Akt3 was little expressed. (jcancer.org)
Human exposures to asbestiform elongate mineral particles (EMP) may lead to diffuse fibrosis, lung cancer, malignant mesothelioma and autoimmune diseases. (cdc.gov)
A Systematic Review of Mesenchymal Epithelial Transition Factor (MET) and Its Impact in the Development and Treatment of Non-Small-Cell Lung Cancer. (lu.se)

Experiments were conducted on human synoviocytes derived from an RA patient and rabbit synoviocytes cell line HIG-82. (aaem.pl)
Overexpression of human epidermal growth factor receptor 2 (HER2) causes cell transformation and development of various types of malignancies. (iranpath.org)
Therefore, the relationship between autophagy and cell death remains unclear and warrants further study to harness autophagy for the treatment of various human diseases. (nature.com)
The present study revealed that miR‑30a‑5p expression was downregulated in human CRC tissues and cells. (spandidos-publications.com)
We tested the effects of Tan II A on melanoma A375, MV3, M14, and other human cell lines including Hacat and HUVEC cells in cell culture model. (biomedcentral.com)
Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
It finds that human osteosarcoma and human lung carcinoma metastasized to bone marrow cell viability decreased to 0% when treated with cNPs. (emerginginvestigators.org)
IL-17F Protein, Human (HEK293, His) is a recombinant human IL-17F protein with His tag at the C-terminus and is expressed in HEK293 cells. (medchemexpress.com)
16-24 h) induces GRO-α, IL-6, and IL-8 secretion in human primary foreskin fibroblast (BJ) cells [5] . (medchemexpress.com)

Due to their secretory functions, differentiation capabilities, specific homing effects through chemotaxis, distinctive therapeutic potentials, and ex vivo expandability, cells have become an attractive reagent for advanced therapeutic strategies. (biomedcentral.com)
Although CNC exposures are associated with pulmonary inflammation and damage as well as oxidative stress responses and genotoxicity in vivo, studies evaluating cell transformation or tumorigenic potential of CNC's were not previously conducted. (cdc.gov)

The review found just small advantages for the gathering welcomed to get the method: an 18% lower hazard of getting colorectal malignant growth, and no huge decrease in the gamble of disease passing. (dalkatimes.com)

In this study, we confirm linc01023 is up-regulated in glioma tissues and cell lines. (jcancer.org)
The viability of malignant colorectal cell lines reduced dose-dependently in the presence of VNPs@Salvia officinalis. (authorea.com)
The effect was strong in epithelioid-dominated cell lines (ZL55, MSTO-211H). (unifr.ch)
The specificity of the effect was confirmed by stable eGFP-CR expression in mesothelioma cell lines and subsequent down-regulation. (unifr.ch)
However, existing mitosis-targeting chemotherapeutic agents do not discriminate between healthy and malignant cell lines, resulting in severe side-effects . (medicalxpress.com)
Luteolin's positive inhibition of melanoma cell lines. (emerginginvestigators.org)
In Chapter 2, the effect of CAP on the metabolic activity of three malignant haematological cell lines, THP-1, U266B1 and U937, was investigated. (edu.au)
CAP induced cytotoxicity in all three cell lines in a concentration-dependent manner. (edu.au)

English Malignant mesothelioma (MM) are highly aggressive asbestos-related neoplasms, which show strong chemotherapy-resistance and there is no effective cure for MM so far. (unifr.ch)
A weaker and delayed effect was observed in mesothelioma cells with prevalent sarcomatoid morphology (SPC111, SPC212, ZL34). (unifr.ch)
Our results demonstrate that down-regulation of CR had a strong effect on the viability of MM cells and that CR is essential for cells derived from malignant mesothelioma. (unifr.ch)
Purpose Malignant pleural mesothelioma (MPM) is a highly lethal neoplasm. (researchtoactionforum.org)
Water-Soluble Truncated Fatty Acid-Porphyrin Conjugates Provide Photo-Sensitizer Activity for Photodynamic Therapy in Malignant Mesothelioma. (shu.ac.uk)

Fibroblast-like synoviocytes: key effector cells in rheumatoid arthritis. (aaem.pl)

The C-terminal of RhoA is modified via prenylation, anchoring the GTPase into membranes, which is essential for its role in cell growth and cytoskeleton organization. (wikipedia.org)
Cancerous growth is characterized as the uncontrolled multiplication and spread of the body's particular cells causing infection and addresses one of the greatest medical care issues for humanity and requests a proactive procedure for fix. (researchsquare.com)
It has been accounted that unusual symmetric cell division is the central point for causing cancerous growth. (researchsquare.com)
New drugs target some of the stepwise genetic mutations the neoplastic cells gain to become masters of their growth and proliferation ( 9 ). (spandidos-publications.com)
Autophagy plays an important role in cell growth, development and disease suppression. (biomedcentral.com)
Furthermore, EDG2 was discovered to promote cell viability and growth of HCC cell through PI3T/AKT/mTOR/Skp2/g27Kip1 signaling. (liveconscience.com)
Quickly, the growth cells (2? (researchtoactionforum.org)
The commitment has been that on the off chance that you get through the ungainliness and obtrusiveness of having a camera venture to every part of the length of your internal organ once consistently after age 45, you have the most obvious opportunity with regards to getting - and maybe forestalling - colorectal malignant growth. (dalkatimes.com)
It's the second most considered normal reason for malignant growth demise in the US. (dalkatimes.com)
It decreased the gamble of colorectal malignant growth by 31% and cut the gamble of passing on from that disease by half. (dalkatimes.com)
In view of his outcomes, then, he expects that screening colonoscopy most likely diminishes an individual's possibilities of colorectal malignant growth by 18% to 31%, and their gamble of death from 0% to as much as half. (dalkatimes.com)

Autophagy is an evolutionary conservative process that maintains cellular homeostasis and viability [ 1 ]. (aging-us.com)
When the cell's DNA and protein damaged, the cells maintained its cellular homeostasis through autophagy. (biomedcentral.com)

Although it seemed that enhanced appearance of EDG2 did not influence TGF1 signalings, EMT transcription factors including SNAI1 and Turn were both found up-regulated by overexpression of EDG2 in Huh7 cells. (liveconscience.com)

The dimeric form of the Z HER2 affibody molecule showed a mild effect on the cell viability of all treated cells at its optimum concentration. (iranpath.org)
However, such an effect was not observed in the cells synchronized and treated with cisplatin at G2/M. (bmj.com)
Transwell chamber experimental was performed to assess the effect of Tan II A on A375 melanoma cell invasion ability. (biomedcentral.com)
The PDT effect of these photosensitizers was initially assessed using the colorimetric WST-8 cell viability assay and the mode of cell death was determined via flow cytometry of Annexin V-FITC/PI-stained cells. (shu.ac.uk)
A biphasic effect on metabolic activity was observed on THP-1 cells [EC50, IC50 (95% CI) = 32.9 (19.9-54.3), 219 (144-246) ˜í¬¿M]. U266B1 cells were more resistant to CAP-induced death than THP-1 and U937 cells. (edu.au)
Subsequently, the effect of an additional small molecular inhibitor, BMS-777607 (which targets MST1R (RON), MET, Tyro3, and Axl) also resulted in a decreased proliferative capacity of MPM cells. (frontiersin.org)

Remarkably, emerging articles have shown that lncRNAs are involved in regulation of glioma malignant behaviors [ 7 , 8 ]. (jcancer.org)

PCD has fundamental functions in tissue development and homeostasis, as PCD is activated to sculpt or remove structures, regulate cell numbers, and eliminate unnecessary or dysfunctional cells. (nature.com)

The constructed Idarubicin-Z HER2 affibody conjugate decreased the viability of SK-OV-3 cells at its optimal concentration, more efficiently and specifically than other treated cells. (iranpath.org)
The results showed that the ethanolic extract of saffron decreased cell viability in malignant cells as a concentration and time-dependent manner. (phcog.com)
Cells were treated with the indicated concentration of siCKAP5-LNP and compared with an equivalent concentration of siControl LNP. (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)

One relatively new approach is to use oncolytic adenoviruses, which selectively infect and kill cancerous cells leaving healthy cells unharmed. (helsinki.fi)
Specific targeting by vanilloid-like agents toward TRPV1 on cancerous cells in patients with haematological malignancies may represent a novel therapeutic approach to treating these diseases. (edu.au)

Conclusions We demonstrated BMS-790052 that S-1 was effective for inhibiting the proliferation of MPM cells, particularly with both DPD and TP expressions, suggesting that S-1 might be therapeutically effective for control of MPM. (researchtoactionforum.org)

Morphology of MCF7 cells treated with the ethanolic extract confirmed the MTT results. (phcog.com)

Background: Tropomyosin 1 alpha chain (Tm1) is an actin-binding protein that regulates the endothelial cell response to oxidative stress following its phosphorylation at Serine 283 (S283). (scirp.org)

Proliferation and cell viability were assayed by means of the enzymatic cleavage of the tetrazolium salt WST-1 to formazan dye by cellular mitochondrial dehydrogenases. (scirp.org)

Cell cycle analysis was carried out using flow cytometry. (bmj.com)

Caco-2 cells with a high expression of both HER2 and FASN were selected for functional characterization. (biomedcentral.com)

Malignant melanoma (MM) is one of the high degree of malignancy and early prone to blood and lymph node metastasis. (biomedcentral.com)
We found that Tan IIA inhibited melanoma A375, MV3, and M14 cell proliferation in dose and time dependent manner. (biomedcentral.com)

Anatomy31

Organisms14

Diseases21

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes65

Disciplines and Occupations1

Information Science3

Health Care1

Vitro17

Apoptosis14

Phenotype8

Evaluated by MTT assay1

Tumors9

Assay and western blot analysis1

Tumor17

Glioma cells2

Survival11

Epithelial Cells3

Significantly7

Cancers5

Western blot2

Cytotoxic7

Migration23

Knockdown4

Tumour cell1

PI3K8

Progression5

Pathway10

Proteins9

Adhesion5

Squamous2

Proliferation of human2

Skin lesions1

Methods3

Cancer48

Human10

Vivo2

Decrease1

Lines8

Mesothelioma5

Effector1

Growth11

Cellular homeostasis2

Overexpression1

Effect6

Regulation1

Regulate1

Concentration4

Cancerous cells2

Conclusions1

Morphology1

Oxidative stress1

Cleavage1

Flow cytometry1

HER21

Melanoma2