Viability cho cells. Medical search. Frequent questions

One of the main problems in mammalian cell culture systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. (mit.edu)
These pathways were de novo synthesized in 3 kilobase chunks, assembled in yeasto and genomically integrated into a Chinese hamster ovary (CHO) cell line. (elifesciences.org)
In the application described herein, the ScanRDI™ assay is intended to detect a low-level bacterial contamination in a background of millions of Chinese Hamster Ovary (CHO) cells. (europeanpharmaceuticalreview.com)
LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
Many biotherapeutic drugs such as recombinant antibodies are manufactured utilizing host cells, a common example being Chinese hamster ovary (CHO) cells. (technologynetworks.com)
Lactate is a major by-product of Chinese hamster ovary (CHO) fed-batch cultures. (technologynetworks.com)
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins. (wikipedia.org)
In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganian's laboratory at the Boston Cancer Research Foundation and used it to derive the original Chinese hamster ovary (CHO) cell line. (wikipedia.org)
Furthermore, Chinese hamster ovary cells are able to produce proteins with complex glycosylations, post-translational modifications (PTMs) similar to those produced in humans. (wikipedia.org)
Optimized for the cultivation of Chinese Hamster Ovary (CHO) cells, along with many derivatives of this parent line, PF Medium is a proprietary serum-free and protein-free growth medium that does not contain any hormones or growth factors. (novabio.ee)
Rational genetic engineering to enhance biotherapeutic proteins has become a reality catalyzed by publication of the genome sequences of multiple Chinese hamster ovary (CHO) cell lines. (bioprocessintl.com)
Development of a safe and high-quality Chinese hamster ovary (CHO) cell line is of paramount importance for the chemistry, manufacturing, and controls (CMC) portion of studies that support investigational new drug (IND) applications (1, 2). (bioprocessintl.com)

However, serum deprivation inhibits cell growth and induces apoptosis. (ewha.ac.kr)
In a previous study, the anti-apoptotic 30K proteins were identified from silkworm hemolymph, which suggests that the 30K genes coding for the anti-apoptotic compound can be used for the anti-apoptosis engineering of mammalian cells. (ewha.ac.kr)
In this study, the 30K genes (30Kc6, 30Kc19, and 30Kc123) were introduced to DG44 CHO cells, which are the mammalian cell line most commonly used by industry for the production of biopharmaceuticals, in order to make them resistant to the apoptosis induced by serum deprivation. (ewha.ac.kr)
When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis. (ewha.ac.kr)
A majority of CHO cells in culture were found to die by apoptosis, a genetically controlled form of cellular suicide. (mit.edu)
Caspases, a class of proteins found to be universally important in inducing apoptosis, were found to be activated in apoptotic CHO cells. (mit.edu)
Using these results, a pathway for apoptosis in CHO cells, focusing on the caspases and bcl-2, was suggested. (mit.edu)
The loss of insulin was concurrent with the accumulation of cells in the GO/G1 state and an increase in expression levels ofp53, a well-documented growth-inhibiting and apoptosis-inducing gene. (mit.edu)
ATZ also induces morphological changes similar to apoptosis and initiates mitochondria-dependent cell death in several experimental models. (frontiersin.org)
Yedjou C, Izevbigie E, Tchounwou P. (2013) Vernonia amygdalina-Induced Growth Arrest and Apoptosis of Breast Cancer (MCF-7) Cells. (nexcelom.com)
The PI staining results showed that D. sawyeri , S. obtusicrena, and C. oxyodon in CHO cancer cells could induce apoptosis in a concentration-dependent manner. (rjpharmacognosy.ir)
Meanwhile, both MH and Ac-DEVD-CHO had similar eﬀects in protecting cell morphology, reducing LDH release, and inhibiting OGD-induced apoptosis in neurons. (syksignal.com)
More physiologic conditions result in uniform and complete post-translational modifications, reduced apoptosis and host cell protein contamination, and for complex cell-to-cell interactions to develop. (cellme.de)

Prior to assay set-up, CHO cells were grown to between 70-90% confluence in T75 tissue culture flasks in 10-20 mL sterile culture media, and spun and detached using trypsin. (genengnews.com)
Replication competence was found to correlate well with viability results from the acridine orange / ethidium bromide assay, but not with results from the trypan blue assay. (mit.edu)
This results in differing cytotoxic responses that are influenced by the nature of target cells, assay types and concentrations of ATZ. (frontiersin.org)
In order to evaluate the premise that the ScanRDI™ assay is comparable to the growth-based membrane filtration assay for the purpose of detecting the presence of bioburden in cell culture samples, a master validation plan was drafted at Wyeth Biotech, Andover MA (USA), including elements of method evaluation, concurrent testing, method validation and crossover studies. (europeanpharmaceuticalreview.com)
In the ScanRDI™ assay, samples are passed through a 0.45μ filter to retain microorganisms, which are then labeled with reagents to determine their viability. (europeanpharmaceuticalreview.com)
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance. (creative-biolabs.com)
The safety profile was evaluated on CHO-k1 epithelial cells viability using the Alamar blue assay, and by acute toxicity test in mice. (researcher-app.com)
Cell viability was quantitated by Alamar blue® assay. (rjpharmacognosy.ir)
Additional fee will be charged for Western blot or cell-based assay of your interest. (welgen.com)
To identify potential intestinal carcinogens, we developed a cell transformation assay using mouse intestinal organoids (mASC-IOs) and assessed the transformation potential on 14 representative chemicals. (cellme.de)

Bcl-2 expression was shown to improve both final product titers and integrated viable cell densities in CHO fed-batch cultures. (mit.edu)
do not let your cells overgrow and do not "over split" your cells by sub culturing the cells at low densities on multiple consecutive days. (cellculturedish.com)
Single Use Disposable BioSettler has been demonstrated as a selective cell retention device for CHO perfusion bioreactors to achieve and maintain high cell densities and high viabilities. (sudhinbio.com)
They represent the only way to culture cells at in vivo like cell densities permitting the cells to generate their own specific microenvironment. (cellme.de)
Inclined settling technology has been successfully demonstrated in biopharmaceutical manufacturing for achieving high cell densities and viabilities for extended culture durations. (cellme.de)

CHO transfected suspension cells have been carried for more than 2 years in IRIS medium with no loss of viability. (thomassci.com)
HEK, CHO cells, and Dextran Microcarrier Beads can be continuously kept in uniform suspension for 8 hours without loss of viability or integrity. (vp-sci.com)
This research aimed to examine the controlled release of sodium butyrate onto CHO-K1 cells, to increase recombinant protein production, while minimizing loss of viability. (thea.ie)

In one of the studies presented in the poster, Essential Pharmaceuticals used their universal titer boost and enhancer, Cell-Ess, to improve protein production in CHO cells using a 5 liter WAVE Bioreactor as the manufacturing vessel. (cellculturedish.com)
Next, Cell-Ess was tested as a feed supplement by conducting feed experiments in both shake flasks and in the WAVE 5 liter bioreactor in CD FortiCHO TM Medium and Feed from ThermoFisher Scientific. (cellculturedish.com)
There is an increase in titer of mAb with the addition of Cell-Ess® in a Wave Bioreactor. (cellculturedish.com)
This review described the factors that affect growth properties and metabolism at elevated oxygen concentrations that cells may be exposed to, in bioreactor sparged with oxygen enriched air which could affect the yield and quality of the recombinant proteins produced by high cell density schemes. (biomedcentral.com)
Therapeutic monoclonal antibodies (MAbs) mostly are manufactured using bioengineered mammalian cells cultured in a bioreactor for two to three weeks. (bioprocessintl.com)
We have developed a novel compact scalable device with smaller footprint and higher inclined settling surface area and fabricated it as a single-use disposable plastic BioSettler for recycling of live and productive CHO cells back to the bioreactor, and continuous removal of dead cells and debris. (cellme.de)
We have now demonstrated it as an inclined settling bioreactor combining the features of a gentle air-lift cell culture bioreactor with the selective cell retention features of the inclined settler, making it useful for growing suspension cells like CHO, IPSC clusters, and attachment dependent cells like MSCs on microcarrier beads and HEK cells. (cellme.de)

The complex genetics of CHO cells and the issues concerning clonal derivation of cell population was extensively discussed. (wikipedia.org)
CHO suspension clonal stable cell lines are generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to express single gene of interest. (welgen.com)
Adsorption and release of butyrate was measured by GC-MS and the effect of sodium butyrate on recombinant protein (human insulin-GFP) production in CHO K1-INS/GFP clonal cells was quantified using fluorescent microscopy. (thea.ie)

To provide high-density cell cultures with sufficient iron, elevated concentrations of iron salts are required that utilize low-affinity nontransferrin receptor pathways. (genengnews.com)
Eliminate risk of animal or viral infectious agents in cell cultures. (pharmaceutical-business-review.com)
This thesis was undertaken to better understand the processes of death in CHO cells and to find ways to extend the viability of CHO cultures. (mit.edu)
In contrast, expression of bcl- 2, a well-characterized anti-apoptotic gene, was able to significantly increase the life of CHO batch cultures in response to both glucose limitation and growth factor withdrawal. (mit.edu)
It was also able to maintain insulin deprived fed-batch cultures in a viable and productive state for much longer than insulin supplemented cultures, thus suggesting an easy way to maintain viability and productivity coupled with slower growth. (mit.edu)
Concentrations of insulin, a growth and survival factor used in CHO culture, were observed to drop rapidly in fed-batch cultures of CHO cells. (mit.edu)
Insulin degradation was sharply reduced by adding sodium glycocholate, an amino-peptidase inhibitor, suggesting that amino-peptidases play a major role in insulin degradation in CHO fed-batch cultures. (mit.edu)
Based on the above results, possible ways to further reduce death and improve productivity in CHO cultures are also suggested. (mit.edu)
Molly Campbell (MC): When producing biotherapeutics, why is it important to use a high-quality medium to facilitate the growth of cell cultures? (technologynetworks.com)
In the Lonza portfolio, we have several media dedicated to CHO cell culturing, including ProCHO TM 5 and PowerCHO TM 2, which many of our users have been using in fed-batch cultures. (technologynetworks.com)
In the development of this new medium, our goal was to offer our customers a paired basal and feed for CHO media fed-batch cultures. (technologynetworks.com)
The supernatant, containing the RL34 cell-secreted growth factors, was used as the conditioned medium (CM). This CM, with CVE added as a nutrient source, was applied to primary bovine myoblast cultures. (nature.com)
The productivity of suspended mammalian cell cultures plays a major role in the overall manufacturing costs of biotechnology products. (upr.edu)
They are easily growable in large-scale cultures and have great viability, which is why they are ideal for GMP protein production. (wikipedia.org)
Cell cultures derived from mammalian and bacterial cell lines are the conventional production systems in bioprocessing. (bioprocessintl.com)
Media for mammalian cultures in particular are notoriously expensive, and traditional cell cultures can be highly sensitive to growing conditions. (bioprocessintl.com)
During the late 1980s and into the 1990s, plants and plant-derived cell cultures were introduced as alternative cell-culture systems (1, 2). (bioprocessintl.com)

One synthetic pathway produced valine at a sufficient level for cell viability and proliferation. (elifesciences.org)
This serum-free and grain-derived-nutrient-free medium promoted the proliferation of bovine myoblasts, the main cell source for cultured beef. (nature.com)
Figure 1) This increase was reached without increasing CHO cell proliferation. (cellculturedish.com)
Originally demonstrated to control the dynamics of the F- actin cytoskeleton , Rho GTPases have been implicated in many basic cellular processes that influence cell proliferation, differentiation, motility, adhesion, survival, or secretion. (xenbase.org)

hiPSC (human induced pluripotent stem cell) are a source of potential cellular immunotherapies. (aicompanies.com)
Ensure stem cells are at the highest viability and product quality levels throughout the entire cell therapy workflow from culture and processing all the way to fill and finish. (aicompanies.com)
2012) Identification, isolation and invitro expansion of human and nonhuman primate T stem cell memory cells. (nexcelom.com)
Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. (mscience.com.au)
2 psi, the WOLF G2 are gentler than any conventional cell sorters, enabling healthier cells post-sort, especially for engineered lines, primary cells, and stem cells. (bucher.ch)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)
Given this, the ALINE MANICA2 cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a JULIANA CRISTINA SCHMIDT2 way to collect them in one surgical procedure and after performing fractional fat grafting. (bvsalud.org)
Cryopreservation of mesenchymal stem cells: Alternative to fat grafting in breast reconstruction análise das CTDAs, após 1 e 3 meses de congelamento, indicou que as amostras tratadas com L-prolina + trealose apresentaram viabilidade semelhante àquelas preservadas com DMSO e SFB ( p =0,444). (bvsalud.org)

First, rat liver epithelial RL34 cells were cultured by adding Chlorella vulgaris extract (CVE) to inorganic salt solution. (nature.com)

Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK 293 or CHO cells, an expression or infection system and selection, followed by process and media optimization in small scale systems such as 96-well plates or bench-scale bioreactors. (beckman.com)
To meet volume demands, biopharmaceutical companies have built large-scale facilities with multiple 10,000 L (or even larger) cell culture bioreactors. (beckman.com)
Most, if not all industrially used CHO cell lines are now cultivated in animal component free media or in chemically defined media, and are used in large scale bioreactors under suspension culture. (wikipedia.org)
Hollow fiber bioreactors provide a fundamentally more in vivo like way of culturing cells. (cellme.de)

11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)
Transfection into mammalian cells is followed by screening for cell lines permanently expressing specific genes through their incorporation into the cellular genome. (welgen.com)
BAPtofect®️-25 is also available with encapsulated florescent dyes to visually confirm cell and tissue transfection. (phoreusbiotech.com)
There was no difference in siRNA knockdown of a target protein in CHO cells following transfection with BAPC vs. two other transfection agents (TransIt-X2 and Lipofectamine 3000). (phoreusbiotech.com)
What cell density do you recommend before transfection in CHO cells? (cellculturedish.com)
Deleterious cell culture conditions can negatively impact not just your next transfection, but all transfections from that point forward. (cellculturedish.com)
At the time of transfection, CHO cells, like most other cell types, should have high viability (95% or greater) and should be in low to mid log phase growth. (cellculturedish.com)
Thus, for a CHO cell line with mid log phase at 4×10^6 viable cells/mL, transfecting cells when they reach a density of approximately 1-4×10^6 would be reasonable, with 2-3×10^6 likely being an optimal target density at the time of transfection. (cellculturedish.com)

When the therapeutic you're developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success. (aicompanies.com)

Other than propidium iodide, other fluorescent membrane exclusion dyes can also be used to measure cell viability. (nexcelom.com)
Chan LL, Wilkinson AR, Paradis BD, Lai N. (2012) Rapid Image-based Cytometry for Comparison of Fluorescent Viability Staining Methods. (nexcelom.com)
The detection and enumeration of microorganisms is achieved by the system through a sequential series of computerised algorithms, which discriminate between fluorescent signal generated by bacterial cells and nonspecific fluorescence generated by debris or other material on the filter membrane. (europeanpharmaceuticalreview.com)
Traditional methods for cell viability assessment use fluorescent dyes or labels to differentiate live cells from dead, but are destructive to cell samples while also adding complexity, time, and risks to the process. (ovizio.com)
Cell culture parameters (pH, dissolved oxygen, and temperature) measured with fluorescent dye sensors inserted from the top of BioSettler and controlled by sparged gas mix of air, oxygen and carbon dioxide manipulated by automation and control system. (sudhinbio.com)

This is important because increasing titer through increased cell density can put a strain on downstream operations. (cellculturedish.com)

Cell viability was determined by the trypan blue exclusion method. (genengnews.com)
The wash step was repeated, and viability determined by trypan blue exclusion method. (genengnews.com)

Cell viability/death was determined with MTT and LDH release assays. (biomedcentral.com)
Owing to the time required for results using traditional bioburden monitoring assays, the cell culture process must continue at risk until the results are available. (europeanpharmaceuticalreview.com)

iPSC matrices to increase cell survival and outgrowth without coating plates. (aicompanies.com)
The talk will describe the generation of a novel developmentally-staged engineered iPSC-derived human ventricular cardiac tissue organoids comprising of all cell-types of native human cardiac ventricles with its concordant molecular, metabolic, structural and physiologic characteristics. (cellme.de)

An increase in iron concentration can have a toxic effect on the cell due to the accumulation of unbound ferric or ferrous ions. (genengnews.com)
If combined with IRIS Feeds medium supports a viable cell concentration up to 3x107/mL depending on clone performance. (thomassci.com)
2013) Accurate measurement of peripheral blood mononuclear cell concentration using image cytometry to eliminate RBC-induced counting error. (nexcelom.com)
The cells of the inner medullary collecting duct (IMCD) are key elements of the nephron in the process of urinary concentration and dilution [ 1 ]. (biomedcentral.com)
A system designed for process flexibility allows users to optimize protocols for cell separation, washing, and concentration, and closed single-use kits enable sterile processing to facilitate scale-up to manufacturing. (labmanager.com)
The CTS Rotea system offers exceptional cell recovery, flexible input and output volume capability, and high throughput, making it ideal for cell separation, concentration, washing, and more. (labmanager.com)
The CTS Rotea Counterflow Centrifugation System is a highly versatile tool that is suitable for cell separation, as well as buffer exchange and cell concentration between cell therapy processing steps. (labmanager.com)
Harvesting, Washing and Concentration of Cell Therapy Products (e.g. (sudhinbio.com)
Sudhin BioSettler can be used for Harvesting, Washing and Concentration of Cell Therapy Products (e.g. (sudhinbio.com)
The activity of caspase-3 was detected, in order to ﬁnd the precise concentration of Ac-DEVD-CHO with the same protective role in OGD injury as MH treatment. (syksignal.com)

It is a recombinant analog of human transferrin that has shown equivalence to serum-derived human transferrin (hTf) and superiority to bovine transferrin (bTf) in stimulating cell growth and productivity for a number of cell lines. (genengnews.com)
Cells were then washed in 10 mL Dulbecco's PBS, spun and resuspended in serum-free, growth factor-free medium. (genengnews.com)
The metabolic dye MTS was added to the remaining media for analysis of viable cell numbers and absorbance was measured from each of the six replicate wells to determine growth. (genengnews.com)
To examine the effect of rTransferrin on CHO cell growth and productivity, two concentrations (1 and 5 µg/mL) of the supplement were tested. (genengnews.com)
5 µg/mL) displayed similar growth and productivity effects in CHO and Sp2/0 cells to iron supplements in common use within the industry, rTransferrin was compared with serum-derived hTf or bTf (each at 5 µg/mL) and three different inorganic iron salts. (genengnews.com)
The medium provides equivalent of higher growth of CHO transfected cells when compared to serum-containing medium. (thomassci.com)
When in vitro experimental models are exposed to ATZ, they are faced with increased levels of reactive oxygen species (ROS), cytotoxicity and decreased growth rate at dosages that may vary with cell types. (frontiersin.org)
The production of MCBs must adhere to cGMP (Clinical Good Manufacturing Process) conditions, aiming to optimize cell growth while ensuring their stability and functionality for safe and effective transplantation into patients. (aicompanies.com)
Adding a simple osmolality measurement to the in-process parameters you measure is a quick and easy way to ensure optimal cell growth, during process development and manufacturing as well as during feed and media preparation. (aicompanies.com)
A key factor for consideration in this process is the growth and viability of these cells - ultimately this influences the quality and quantity of the biotherapeutic product. (technologynetworks.com)
Here, we developed a novel medium containing nutrients extracted from microalga and cell-secreted growth factors. (nature.com)
Animal cells are generally cultured in basal media supplemented with fetal bovine serum (FBS) which provides the various factors essential for cell growth. (nature.com)
The culture supernatant of animal cells, also known as 'conditioned medium' (CM), contains various factors secreted by the cells themselves, such as growth factors and cytokines. (nature.com)
Producing highly productive cell lines and optimized cell culture conditions requires an understanding of the contributions of many key variables: cell culture media, cell growth rates, cell viability, protein expression levels, and other quality attributes including size, aggregation status, glycosylation and stability. (beckman.com)
2011. Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7. (pdfslide.us)
Since then, CHO cells have been a cell line of choice because of their rapid growth in suspension culture and high protein production. (wikipedia.org)
Also, CHO cells do not express the epidermal growth factor receptor (EGFR), which makes them ideal in the investigation of various EGFR mutations. (wikipedia.org)
With the routine utilization of high density growth of microorganisms and mammalian cells for production of various biologicals, the use of oxygen-enriched air has become a common strategy. (biomedcentral.com)
The growth-inhibitory effect of hyperbaric oxygen was partially reduced by the addition of yeast extract to the culture medium and it was later established that 10 specific amino acids, niacin, and thiamin were necessary to maintain the cells growth capability at the elevated oxygen concentrations [ 14 ]. (biomedcentral.com)
But even with amino acid supplementation, the growth of E. coli AB1157 in glucose minimal medium was impaired when the cells were exposed to 1 atm of O 2 , and the growth was more affected when the tricarboxylic acid (TCA) cycle-dependent carbon source succinate was used [ 16 ]. (biomedcentral.com)
This medium supports superior growth and viability over long-term passages in both adherent and suspension culture, and is formulated without L-glutamine. (novabio.ee)

Protein synthesis inhibition in CHO cells led to rapid death, indicating that CHO cells were pre-disposed to death and that survival proteins needed to be continuously synthesized to protect cells from death. (mit.edu)
Surprisingly, inhibition of caspase activity using z-VAD.fmk, a universal peptide inhibitor of caspases, failed to significantly extend viability in batch culture although it did prevent cleavage of known intracellular caspase substrates. (mit.edu)

The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms. (creative-biolabs.com)
When combined with the N1 Single-Cell Dispenser accessory, it allows higher rates in single cell detection in wells compared to cell printers or limited dilution methods. (mscience.com.au)
Ovizio's patented double differential digital holographic microscopy (D3HM) circumvents the issues surrounding traditional methods by capturing images of cells without the need for dyes or labels. (ovizio.com)
This genetic selection scheme remains one of the standard methods to establish transfected CHO cell lines for the production of recombinant therapeutic proteins. (wikipedia.org)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)

This review focuses on the differential responses of cell types to ATZ toxicity, testicular effects of ATZ in both in vitro and in vivo models and chemopreventive strategies, so as to highlight the current state of the art on the toxicological outcomes of ATZ exposure in several experimental model systems. (frontiersin.org)
Cultured meat is produced by culturing/proliferating animal muscle cells in vitro. (nature.com)
In vitro and in vivo experiments demonstrate that the synergistic PDT/chemotherapy modality achieves highly efficient cancer cell killing and tumor ablation without undesirable side effects. (bvsalud.org)
In vitro cell culture model. (cytion.com)
Desirable attributes of a CHO cell line include its ability to produce high titers of biotherapeutic proteins facilitate quick recoveries and selection processes maintain phenotypic and genetic stability throughout in-vitro aging of a culture. (bioprocessintl.com)

The cell confluence is measured at 83.7% with a cell density of 102.49×10 3 cells/mm 2 . (ovizio.com)
Also, CHO cells are tolerant to variations in parameters, be it oxygen levels, pH-value, temperature or cell density. (wikipedia.org)
Due to their reduced cell density, dead cells can be removed to optimize viability before delivery. (labmanager.com)

Moreover, transcriptomes of the valine-prototrophic cell mirrored transcriptomes captured during recovery from valine deprivation in parental, valine-auxotrophic counterparts. (elifesciences.org)
In this re- search, hippocampal neurons were cultured and treated with mild hypothermia and Ac-DEVD-CHO after oxygen- glucose deprivation (OGD). (syksignal.com)

2 psi, the WOLF's microfluidic channel is gentler than any conventional cell sorter or flow cytometer, allowing for higher populations of healthy, sorted cells with improved cell viability. (mscience.com.au)

Increasing the dosage of downstream ilvD boosted pathway performance and allowed for long-term propagation of second-generation cells in valine-free medium at 3.2 days per doubling. (elifesciences.org)
We found that MH (32 °C) and Ac-DEVD-CHO (5.96 μMol/L) had equal eﬀects on blocking the activation of caspase-3 and the OGD-induced cleavage of PARP, but neither had any eﬀect on the activation of caspase-8, which goes on to activate caspase-3 in the apoptotic pathway. (syksignal.com)

By comparing the protective eﬀects of mild hypothermia and Ac- DEVD-CHO (a caspase-3 inhibitor) against OGD, our current study was designed to determine whether inhibiting the activation of caspase-3 is involved in the main and common molecular mechanism of mild hy- pothermia in protecting hippocampal neurons from OGD injury. (syksignal.com)

While the functionalization of methionine, threonine, and isoleucine synthesis was unsuccessful, restoration of valine synthesis rendered mammalian cells partially independent of exogenous valine. (elifesciences.org)
All CHO cell lines are deficient in proline synthesis. (wikipedia.org)

Historically, reagents that provided lipid supplements have had varying success on optimizing cell culture media and there have been issues reported in the literature around the use of certain lipids with certain plastics (1). (cellculturedish.com)

The thrombolytic medication alteplase (Activase), which was approved by the US Food and Drug Administration in 1987, was the first commercially available recombinant protein produced from CHO cells. (wikipedia.org)

Elevated lactate concentrations in cell culture media lower the pH which has a negative effect on cell health and viability. (technologynetworks.com)
The cancer cells were cultured in RPMI-1640 and incubated with different concentrations of the plant extracts. (rjpharmacognosy.ir)
These data show the increase in protein over the course of the experiment with the addition of several different concentrations of Cell--Ess. (cellculturedish.com)
However, in most the reported studies, the oxidative stress was caused by challenging the organisms with H 2 O 2 and redox-cycling drugs, but not by subjecting the cells to high concentrations of molecular oxygen. (biomedcentral.com)
In this review we summarize available information about the effect of elevated oxygen concentrations on the physiology of microorganisms and cells at various culture conditions. (biomedcentral.com)

Sheffield Bio-Science is a leader that demonstrates excellence in delivering consistent, high yielding, customer- specific solutions for the cell nutrition and excipient markets. (pharmaceutical-business-review.com)
Using our paired eCHO TM Basal Medium and eCHO TM Feed enables high CHO cell viability during production, resulting in easy downstream processing combined with low lactate levels resulting in high protein integrity. (technologynetworks.com)
The WOLF was designed and created by a team of scientists and engineers who wanted to solve a classic challenge in biological research: how to sort cells of high quality effectively and easily. (mscience.com.au)
More recently, other selection systems have become popular and with vector systems that can more efficiently target active chromatin in CHO cells, antibiotic selection (puromycin) can be used as well to generate recombinant cells expressing proteins at high level. (wikipedia.org)
Since CHO cells have a very high propensity of genetic instability (like all immortalised cells) one should not assume that the names applied indicate their usefulness for manufacturing purposes. (wikipedia.org)
However, high costs and time requirements, especially for experiments at the single cell scale, make it challenging for this approach to meet the demand for increased throughput. (authorea.com)
NutriFreez® D5 ensures high viability, recovery rates, and performance even for extremely sensitive cells. (technologynetworks.com)
High temperatures and an altered redox environment may compromise the quality of MAbs produced (e.g., fragmentation, truncation), as can the presence of proteases, reductases, and other chemicals released from dead cells. (bioprocessintl.com)
These organoids exhibit the necessary vascularization and innervation to interrogate, at high resolution, the contribution of mutant immune cells on cardiac inflammation and consequent heart failure. (cellme.de)

The SIECm exhibited no cytotoxic effects on the CHO-k1 cells, and no oral acute toxicity in mice. (researcher-app.com)
The Methanol extract of D. sawyer and L. album had cytotoxic effects on B16-F10 cells, whereas no toxicity was observed in MCF -7, Hep - G2 and PC-3 cell lines after incubation of the cancer cells with the plant extracts. (rjpharmacognosy.ir)

Cell viability was quantified using the dyes neutral red, propidium iodide and Hoechst staining. (thea.ie)

The qMod is a convenient and fast solution for direct cell counting in transparent supports, from petri dishes, flasks and multi-well plates to multi-layer vessels. (ovizio.com)
In the pictures below, MRC5 & VERO cell lines are cultivated in T75 flasks. (ovizio.com)
First, they looked at the use of Cell-Ess as an initial supplement and the study was done in triplicate using CHO cells in shake flasks using SAFC Platform Media by Sigma-Aldrich. (cellculturedish.com)
Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). (cytion.com)
Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. (cytion.com)
Resuspend the cells carefully in 10 ml fresh cell culture medium and transfer them into two T25 cell culture flasks. (cytion.com)
One or two cell culture flasks come filled with cell culture medium. (cytion.com)

Live cells can be damaged by conventional cell sorters, resulting in a low-quality cell population. (mscience.com.au)

CHO cells have played a significant role in the production of recombinant protein therapeutics since, with CHO cells producing 70% of biologics and monoclonal antibodies in 2016. (wikipedia.org)

The increase in titer, yield per cell, and productivity were then calculated and represented as a percent increase over control. (cellculturedish.com)

The new WOLF G2 instrument has significantly expanded the capabilities of gentle benchtop microfluidic cell sorting with two lasers and up to nine colors , while maintaining simple workflows for either bulk sorting or single-cell dispensing. (bucher.ch)
The methanol extracts of D. sawyeri , S. obtusicrena, and C. oxyodon significantly decreased the viability of CHO cells. (rjpharmacognosy.ir)
Our faster and more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity. (welgen.com)
Total cells, macrophages, and eosinophils recovered in BALF lavage were significantly elevated in the OVA + PM/OVA compared to OVA/OVA group. (cdc.gov)

This provides users with access to the D3HM technology, allowing them to take advantage of the dye-free setup to monitor cell viability, cell confluency, cell morphology. (ovizio.com)
Control the cell morphology and confluency under the microscope. (cytion.com)

Developed by bioprocess engineers to improve cell culture performance and to enhance protein production while maintaining cell viability. (pharmaceutical-business-review.com)
The medium is developed for maximal recombinant protein production in suspension cells. (thomassci.com)
Developed to maximize recombinant protein production of CHO transfected cells. (thomassci.com)
IRIS medium is dedicated to maximal production of recombinant proteins with CHO DG44 cells. (thomassci.com)
Timely results for microbial bioburden monitoring of in-process cell culture samples are critical to the production process for recombinant proteins and other biopharmaceutical products. (europeanpharmaceuticalreview.com)
In the poster, Dr. Adam Elhofy, Ph.D. described a series of studies that examined the use of a lipid supplemented media enhancer to increase protein production at various stages and in different cell culture scenarios. (cellculturedish.com)
Cell-Ess was able to achieve a greater than 25% increase in protein production using their supplement in this system. (cellculturedish.com)
In their poster they describe a series of studies designed to investigate whether the use of Cell-Ess could improve protein production as an initial supplement and also as a feed when added to a previously optimized system. (cellculturedish.com)
At 1% Cell-Ess there is a significant increase in mAb production. (cellculturedish.com)
Results of the shake flask study showed that Cell-Ess provided an increase in yield of 25% and extended the window for peak protein production from 1 day to 2 days (Figure 2). (cellculturedish.com)
In this experiment, the use of Cell-Ess extended the production to 17 days. (cellculturedish.com)
CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. (wikipedia.org)
Due to this, CHO cells lacking DHFR are the most widely used CHO cells for industrial protein production. (wikipedia.org)
which are likely to be active under the conditions of increased protein production by controlled butyrate exposure in comparison to bolus dosing, are proposed as mechanisms for improved cell survival. (thea.ie)
With 20 years of experience in the biopharmaceutical industry - at Genentech, Applied Biosystems, Cell Genesys, Cellerant Therapeutics, and Bayer - Yuval Shimoni has written frequently for BioProcess International on a number of production topics. (bioprocessintl.com)

Phase images of VERO cells in a T75 flask, without (left) and with cell segmentation (right). (ovizio.com)

In addition, we found that the presence of BAPC did not affect cell viability after one month of cryopreservation. (phoreusbiotech.com)
A salt-based, chemically defined, animal component-free, protein-free cryopreservation solution, optimal and specially designed for the freezing and thawing of cells intended for cell therapies and clinical applications. (technologynetworks.com)

These experiments proved that early apoptotic cells, which have lost membrane integrity but not chromatin integrity, can be considered dead since they lose the ability to replicate. (mit.edu)
NanoCellect's microfluidic cell sorter, the WOLF, allow the cell's integrity to be maintained for numerous sequencing and multi-omics techniques. (mscience.com.au)
The cartridges employ a piezoelectric actuator and operates at less than 2 psi allows for gentle sorting that preserves viability and RNA integrity. (bucher.ch)

Many attempts have been made to develop a serum-free medium on account of the problems caused by serum in mammalian cell culture. (ewha.ac.kr)
Sheffield Cell Culture capabilities include animal-component free supplements for use in a variety of cell lines. (pharmaceutical-business-review.com)
In addition, the experiments proved that bcl-2 expression is able to extend the replication competence of cells under normal culture conditions. (mit.edu)
Insulin degrading activity was found to be at least partially caused by release of proteases from cells into the culture medium. (mit.edu)
One type of cell culture manufacturing process, called a batch refeed process, can be used to demonstrate two main aspects of the cell culture operation which will further exemplify the importance of timely results. (europeanpharmaceuticalreview.com)
Furthermore, although a monoculture of mammalian cells is expected in the cell culture process, the presence of a certain level of bioburden can be tolerated with validated removal systems in place during downstream purification (sterility is not a requirement at this stage, where the presence of bioburden is an operational concern for the process). (europeanpharmaceuticalreview.com)
A different experimental approach by West and Cooke [ 10 ] demonstrated the secretion of M r 80-90K hCG-LH receptor complex into the culture media from ligand-induced rat and mouse leydig cells. (biomedcentral.com)
The culture medium composition can have a major impact on the health of the cells and the level of lactate produced. (technologynetworks.com)
We hypothesized that by selecting optimal animal cells and using their CM to culture animal myoblasts, the cells could proliferate without the need for animal sera. (nature.com)
When the final cell culture process is established, companies move forward with the commercialization process which includes process characterization, scale-up, technology transfer and manufacturing process validation. (beckman.com)
2007. Initial identification of low temperature and culture stage induction of miRNA expression in suspension CHO-K1 cells. (pdfslide.us)
together with toxicity effects in CHO-K1 cell culture. (fapesp.br)
This sort of system requires no special mutation, so that non-DHFR-deficient host cell culture have been found to produce excellent levels of proteins. (wikipedia.org)
Clarification of Cell Culture Broth to recover secreted products (e.g. antibodies, AAV vectors, etc. (sudhinbio.com)
Cell culture was established from the left tumor which showed fetal rhabdomyomatous histology as described by Royer-Pokora 2010. (cytion.com)
Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. (cytion.com)
Carefully open the vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of culture medium (room temperature). (cytion.com)
Carefully add 5 ml of cell culture medium to each T25 cell culture flask. (cytion.com)
If a cell pellet is visible, resuspend the cells in 5 ml of cell culture medium and transfer to a T25 cell culture flask. (cytion.com)

For the bolus dose data, the viability at 48hrs was 99.70% for 5mM, 98.36% for 10mM and 93.20% for 25mM, indicating a moderating effect of controlled release on butyrate associated toxicity. (thea.ie)

In 1957, CHO-K1 was generated from a single clone of CHO cells. (wikipedia.org)

The entire surface of the membrane is then analysed by the ScanRDI™ laser scanning cytometer, which detects and counts any labeled cell on the filter. (europeanpharmaceuticalreview.com)
Membrane Target Systems: Relaxin RXFP3 (human) membrane preparation, in CHO-K1 cells. (perkinelmer.com)

The apoptotic cells were determined by PI coloring and Flow Cytometry (Sub-G1 peak). (rjpharmacognosy.ir)

Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins. (welgen.com)
Cells used for transient protein expression should be meticulously maintained from the time of thaw throughout their useful lifespan. (cellculturedish.com)

Influence of Aspergillus fumigatus conidia viability on murine pulmonary microRNA and mRNA expression following subchronic inhalation exposure. (cdc.gov)

Creative Biolabs developed and successfully established a stable cell line, which can stably overexpress the Human CD16B protein. (creative-biolabs.com)
Stable cell line development study report. (welgen.com)

Moreover, adapting host cells to the serum-free medium is difficult and time-consuming. (ewha.ac.kr)
Traditionally, transferrin has been supplied in the form of serum-derived human transferrin (hTf), bovine transferrin (bTf), or, more recently, with the move toward serum-free medium (SFM), inorganic iron salts have been used to supply iron to mammalian cells. (genengnews.com)
Biotherapeutics manufacturers have long moved away from using serum for culturing cells. (technologynetworks.com)
Essential Pharmaceuticals' Cell-Ess product is a serum-free, animal-free universal titer boost and enhancer. (cellculturedish.com)

Additional techniques include MR spectroscopy (MRS), which allows clinicians to characterize the chemical composition of the mass by determining the presence and/or alteration of components such as lactate, N -acetylaspartate (NAA), choline (Cho), and myo-inositol (Ins). (medscape.com)
The lower the amount of the lactate by-product, the healthier the cells will be. (technologynetworks.com)

Anatomy9

Organisms2

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment12

Phenomena and Processes37

Information Science3

Health Care1

Chinese Hamst12

Apoptosis13

Assay10

Densities5

Loss of viability3

Bioreactor7

Clonal3

Cultures17

Proliferation4

Stem8

Epithelial1

Bioreactors4

Transfection8

Monitoring to optimize1

Fluorescent5

Increased cell density1

Trypan blue2

Assays2

IPSC2

Concentration10

Growth21

Inhibition2

Methods5

Vitro5

Density3

Deprivation2

Gentler than any conventional cell1

Pathway2

Inhibitor1

Synthesis2

Reagents1

Commercially1

Concentrations5

High9

Cytotoxic2

Dyes1

Flasks7

Conventional cell sorters1

Monoclonal antibodies1

Productivity1

Significantly4

Morphology2

Production15

VERO1

Cryopreservation2

Integrity3

Culture19

Toxicity1

Clone1

Membrane2

Apoptotic cells1

Protein expression2

Murine1

Stable Cell Line2

Serum4

Lactate2