ProliferationSkeletal muscle regenerationDifferentiationPrecursor cellsRegenerationAdultStromalFibroblastsSatellite cellsPathwaysMigrationTumorReceptorAssaysMurineTissuesVivoMiceTypesTargetsLinesPotentialExpressionMesenchymal stemTumor cellSkeletal muscleRegulatesGenesStemSignificantlyFibersSarcomaPROTEINPopulation
Proliferation10
- However, only miR-1-2 was involved in abnormal proliferation in EVI1 expressing cell lines. (wikipedia.org)
- Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
- Sonic 125256-00-0125B11 hedgehog (Shh) is a forty five-kDa potent signaling protein that regulates the proliferation, differentiation, and cellular patterning throughout a extensive selection of cell types [2,3]. (acthreceptor.com)
- Focal adhesion kinase (FAK) is a one hundred twenty five-kD non-receptor tyrosine kinase that plays a main position in mediating sign transduction by integrins, as effectively as by growth aspect receptors, in the regulation of cell adhesion, migration, proliferation, and differentiation in a range of mobile varieties [12,thirteen,fourteen]. (acthreceptor.com)
- A novel FAK Tyr397 inhibitor suppresses osteoblast proliferation and differentiation, as well as osteoclast formation, via PTHrP-induced RANKL expression in murine bone stromal ST2 cells [twenty]. (acthreceptor.com)
- Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. (koreascience.kr)
- Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. (koreascience.kr)
- Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. (koreascience.kr)
- Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. (koreascience.kr)
- SFK inhibition also induced muscle differentiation in RMS cells by affecting the NOTCH3 receptor-p38 mitogen-activated protein kinase (MAPK) axis, which regulates the balance between proliferation and differentiation. (oncotarget.com)
Skeletal muscle regeneration2
- Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
- Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Differentiation7
- There is evidence that the control of smooth muscle cell differentiation by MiR-1 may be mediated by the down regulation of Kruppel-like factor 4 (KLF4), since a MiR-1 recognition site is predicted in the 3' UTR of KLF4 and inhibition of MiR-1 results in reversed down-regulation of KLF4 and an inhibition of smooth muscle cell differentiation. (wikipedia.org)
- Since the tumor results from undifferentiated cells, agents that promote differentiation hold promise as possible therapies. (wikipedia.org)
- Expression of miR-1 but not miR-133a gave transcriptional profiles that were consistent with a strong promyogenic influence on the cells, again demonstrating the role of miR-1 in muscle differentiation from precursor stem cells. (wikipedia.org)
- All of these observations suggest that mis-regulation of miR-1 and miR-133a can result in tumorogenesis via abolition of the suppressive effect they have on certain gene targets and of the removal of the promotion of differentiation of the cells exerted my miR-1. (wikipedia.org)
- It was described that hedgehog proteins right act on osteogenic precursor cells and osteoblasts to stimulate osteogenic differentiation [7]. (acthreceptor.com)
- Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. (koreascience.kr)
- Overall, our findings suggest that SFK inhibition, besides reducing RMS cell growth and invasive potential, could also represent a differentiation therapeutic strategy for RMS. (oncotarget.com)
Precursor cells1
- This highly malignant tumor has traditionally been considered to arise from committed skeletal muscle precursor cells that fail to differentiate [ 1 ], although some RMSs develop in organs lacking a skeletal muscle component [ 2 , 3 ]. (oncotarget.com)
Regeneration2
- The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
- Furthermore, the implantation of Shhtransduced cells increased the bone regeneration in a rabbit product of calvaria problems [8]. (acthreceptor.com)
Adult1
- Eph continues to be expressed in the larval CNS and imaginal discs, as well as pupal and adult stages as assayed by Northern blot analysis. (sdbonline.org)
Stromal1
- To identify targets that further increased p38i efficacy, we utilized a stromal labeling approach and single-cell RNA sequencing. (bvsalud.org)
Fibroblasts2
- SI221, which showed to be mainly effective against the SFK member YES, significantly reduced cell viability and induced apoptosis, without affecting non-tumor cells, such as primary human skin fibroblasts and differentiated C2C12 cells. (oncotarget.com)
- In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
Satellite cells4
- Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
- Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
- Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. (koreascience.kr)
- In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. (koreascience.kr)
Pathways1
- The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Migration2
- ets1 is involved in extracellular matrix (ECM) degradation which is an important process required for tumor cell invasion and migration. (wikipedia.org)
- Moreover, SI221 decreased in vitro cell migration and invasion and reduced tumor growth in a RMS xenograft model. (oncotarget.com)
Tumor3
- T cells recognize tumor-derived mutated peptides presented on MHC by tumors. (bvsalud.org)
- We showed that the antigenic landscape of the tumors inversely reflected the historical T cell responses in the tumor-bearing patients. (bvsalud.org)
- We show that p38MAPKα inhibition (p38i) limits tumor growth by reprogramming the metastatic tumor microenvironment in a CD4+ T cell-, IFNγ-, and macrophage-dependent manner. (bvsalud.org)
Receptor2
- which promoted receptor activator for nuclear factor B ligand (RANKL). (acthreceptor.com)
- EphA1 mis-expression did not cause neurodegeneration, shorten lifespan or affect memory but flies mis-expressing the wild-type or mutant receptor were hyper-aroused, had reduced sleep , a stronger circadian rhythm and increased clock neuron activity and excitability. (sdbonline.org)
Assays1
- ChIP assays have shown that EVI1 binds strongly to the promoters of miR-1-2 and miR-133-a-1, and expression of EVI1 is significantly correlated with the expression of miR-1-2 and miR-133-a-1 in established cell lines and in patient samples. (wikipedia.org)
Murine2
- We predicted that highly antigenic tumors with poor antitumor T cell responses, such as osteosarcomas, would be responsive to T cell-based immunotherapy regimens and demonstrated this in a murine osteosarcoma model. (bvsalud.org)
- SHH stimulates osteoclast formation with PTHrP in a co-lifestyle technique consisting of ST2 cells and murine CD11b+ bone marrow cells [ten]. (acthreceptor.com)
Tissues2
- First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). (koreascience.kr)
- Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
Vivo2
- FAK deficiency in osteoblasts and osteocytes in vivo benefits in delayed bone healing and transforming and interrupts the response of bone marrow cells to anabolic mechanical stimuli in a tibial injuries product [seventeen,18]. (acthreceptor.com)
- In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)
Mice1
- The combination of p38i, anti-OX40, and cytotoxic T-cell engagement cured mice of metastatic disease and produced long-term immunologic memory. (bvsalud.org)
Types1
- Thousands of copies of the circular mtDNA are present in most cell types that are packaged by TFAM into higher-order structures called nucleoids1. (regenerativemedicine.net)
Targets1
- A study showed that levels of mir-1 and mir-133a were drastically reduced in tumourous cell lines whilst their targets were up-regulated. (wikipedia.org)
Lines2
- These two microRNAs have been shown to downregulate the expression of the ets1 proto-oncogene in cell lines HepG2 by targeting the 3'UTR of ets1. (wikipedia.org)
- Here, we assessed the effect of a recently developed selective SFK inhibitor (a pyrazolo[3,4- d ]pyrimidine derivative, called SI221) on RMS cell lines. (oncotarget.com)
Potential1
- Our study presents a potentially novel pipeline for determining antigenicity of human tumors, provides an accurate predictor of potential neo-epitopes, and will be an important indicator of which cancers to target with T cell-enhancing immunotherapy. (bvsalud.org)
Expression3
- The miR-1 microRNA precursor is a small micro RNA that regulates its target protein's expression in the cell. (wikipedia.org)
- There is evidence for the role of miR-1-2 as a modulator in acute myeloid leukemia via its transcription by the zinc-finger transcription factor, EVI1, ectopic virus expression site 1. (wikipedia.org)
- In the first stages of fracture fix, the expression of sonic hedgehog is detected in proliferating callus-forming cells in the periosteum [6]. (acthreceptor.com)
Mesenchymal stem2
- METHODS: Cell lines from five main sarcoma types of the musculoskeletal system (chondrosarcoma, osteosarcoma, Ewing sarcoma, fibrosarcoma and rhabdomyosarcoma) as well as their healthy cell counterparts (chondrocytes, osteoblasts, mesenchymal stem cells, fibroblasts, skeletal muscle cells) were subjected to cell stiffness measurements via atomic force microscopy (AFM). (bvsalud.org)
- Recent advances in adipogenesis had provided insights into understanding of the complex cues for influencing the cytoarchitecture, epigenomic remodeling, signaling pathways and transcription regulators on gene actions for both white and brown adipogenic progression from mesenchymal stem cells to matured committed adipocytes. (scirp.org)
Tumor cell2
- ets1 is involved in extracellular matrix (ECM) degradation which is an important process required for tumor cell invasion and migration. (wikipedia.org)
- These results suggest that MC delivery via microvesicles can mediate gene transfer to an extent that enables effective prodrug conversion and tumor cell death such that it comprises a promising approach to cancer therapy. (regenerativemedicine.net)
Skeletal muscle3
- Skeletal muscle satellite cells are heavily involved in the regeneration of skeletal muscle in response to the aging-related deterioration of the skeletal muscle mass, strength, and regenerative capacity, termed as sarcopenia. (hindawi.com)
- The regeneration capability of skeletal muscle to adapt with the normal physiology growth and to compensate with the wear and tear of skeletal muscle due to injury or disease is highly dependent on a population of quiescent progenitors, known as satellite cells [ 3 , 4 ]. (hindawi.com)
- Unfortunately, neither skeletal muscle nor the satellite cells could avoid the fate of various modifications during aging. (hindawi.com)
Regulates2
- The miR-1 microRNA precursor is a small micro RNA that regulates its target protein's expression in the cell. (wikipedia.org)
- HN - 2006(1998) MH - Activating Transcription Factor 1 UI - D051697 MN - D12.776.260.108.61.500 MN - D12.776.930.127.61.500 MS - An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2. (nih.gov)
Genes1
- The authors propose that miR-1 and miR-133a act to repress isoforms of genes that are not normally expressed in muscle cells. (wikipedia.org)
Stem1
- Since the specialty of Plastic Surgery with its position statement on stem cells and fat grafting is committed to advancing evidence-base preclinical and clinical studies in compliance with FDA-regulations, an updated review of cell biology provides insights to achieve these goals. (scirp.org)
Significantly4
- ChIP assays have shown that EVI1 binds strongly to the promoters of miR-1-2 and miR-133-a-1, and expression of EVI1 is significantly correlated with the expression of miR-1-2 and miR-133-a-1 in established cell lines and in patient samples. (wikipedia.org)
- RESULTS: While AFM stiffness measurements showed that the majority of cancer cells (osteosarcoma, Ewing sarcoma, fibrosarcoma and rhabdomyosarcoma) were significantly less stiff than their corresponding non-malignant counterparts (p (bvsalud.org)
- Notably, whereas a decrease in stiffness is typically a feature of malignant entities, chondrosarcoma cells were stiffer than chondrocytes, with chondrosarcoma cells exhibiting a significantly upregulated ß-tubulin expression. (bvsalud.org)
- We demonstrated that MCs can be loaded into shed microvesicles with greater efficiency than their parental plasmid counterparts and that microvesicle-mediated MC delivery led to significantly higher and more prolonged transgene expression in recipient cells than microvesicles loaded with the parental plasmid. (regenerativemedicine.net)
Fibers2
- Microscopically, the distribution of F-actin differed between malignant entities and healthy counterparts: the organisation in well aligned stress fibers was disrupted in cancer cell lines and the proteins was mainly concentrated at the periphery of the cell, whereas ß-tubulin had a predominantly perinuclear localization. (bvsalud.org)
- The quiescent satellite cells were activated in response to damage or exercise and proliferated as myoblasts, which further differentiated and fused to repair or form the muscle fibers [ 7 ]. (hindawi.com)
Sarcoma1
- While the F-actin content was lower in cancer cells, particularly Ewing sarcoma (p = 0.018) and Fibrosarcoma (p = 0.023), this effect was even more pronounced in the case of ß-tubulin for all cancer-healthy cell duos. (bvsalud.org)
PROTEIN3
- Interestingly, chondrosarcoma cells were characterized by a significant upregulation of ß-tubulin gene expression (p = 0.005) and protein amount (p = 0.032). (bvsalud.org)
- It is expressed at higher levels than ARP2 PROTEIN and does not contain a PROFILIN binding domain. (nih.gov)
- Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
Population1
- as its loss of function resulted in a reduction in smooth muscle cell biomarkers and a reduction in the derived smooth muscle cell population. (wikipedia.org)