Viability assay spheroids. Medical search. Frequent questions

Regardless of monolayer or spheroid cell culture, PLATMC-HPLG constructs represent promising scaffolds for bone tissue engineering applications. (biomedcentral.com)
They first harvested 2D monolayer cultures of pancreatic epithelial tumor cells that express either wild-type KRAS or mutant oncogenic KRAS, and tested their ability to form 3D spheroids. (promegaconnections.com)
It's important to use the 3D version of the reagent since spheroids typically require stronger lytic reagents than what is required for a 2D monolayer assay. (cellculturedish.com)
We offer assay formats using traditional monolayer models, as well as 3D cell culture models, which better recapitulate the tumor microenvironment in vivo . (visikol.com)
In addition to standard measures of cancer drug efficacy such as anti-proliferative effect, and assessment of cell viability after treatment, the use of 3D cell models provides access to more complex measures that are difficult to assess in standard monolayer formats. (visikol.com)
Impact of an anti-cancerous drug was investigated on colon cell lines (HT-29, HCT116 and LS174T) in conventional 2D monolayer and 3D spheroid cultures, in hypoxia or normoxia. (dynamics365commerce.ms)
You can't just move from the monolayer to the spheroid without optimizing for 3D. (corning.com)

Based on this method, we performed cytotoxicity assays on 42 AsPCs grown both as monolayers and spheroids. (biomedcentral.com)
Viability/cytotoxicity assays indicate dose-dependent cytotoxic effects, which are inhibited by the nitric oxide synthase (NOS) inhibitor L-NIO, and genetic inhibition of endothelial NOS, implicating peroxynitrous acid as a key damaging agent. (ku.dk)

Anti-invasive and anti-multicellular tumor properties were evaluated by cellular network and spheroid formation models, respectively. (biomedcentral.com)
Boswellia sacra essential oil hydrodistilled at 100 o C was more potent than the essential oil prepared at 78 o C in inducing cancer cell death, preventing the cellular network formation (MDA-MB-231) cells on Matrigel, causing the breakdown of multicellular tumor spheroids (T47D cells), and regulating molecules involved in apoptosis, signal transduction, and cell cycle progression. (biomedcentral.com)
This assay provides an in-vitro standardized 3D high content format for inducing the formation of multicellular tumor spheroids and quantitating the cell viability in response to the drug treatment. (labcritics.com)

MiRNAs-based transcriptome sequencing and data analysis, online dataset analysis, luciferase reporter assay combined with rescuing experiments were constructed to explore the underlying mechanisms. (biomedcentral.com)
In this study, we applied western blot, quantitative real-time polymerase chain reaction (qRT-PCR), immunohistochemistry, RNA-Seq analysis, luciferase reporter assay, Chip-qPCR, bioinformatics analysis, and a series of functional assays to show the potential role of LEF1 in regulating esophageal CSCs. (biomedcentral.com)

They confirmed spheroid growth using the CellTiter-Glo® 3D Cell Viability Assay with linearity of detection in the range of 1,000-10,000 cells seeded. (promegaconnections.com)
A good homogenous method for assessing viability is using Promega's CellTiter-Glo 3D reagent to measure ATP. (cellculturedish.com)

Conventional in vitro assays are based on cells grown on two-dimensional (2D) substrates, which are not representative of a true in vivo cell environment. (technologynetworks.com)
The material from each step isscreened using acellular and in vitro assays for evaluating general toxicity, mechanisms of toxicity, and macrophage function. (cdc.gov)

AMSBIO , one of the leading providers of cutting edge products and services for the life science industry has launched a new range of 3D assay for evaluating cell proliferation and viability. (labcritics.com)
The new AMSBIO 3D Spheroid Proliferation/Viability Assay kit uses 3D Culture Quantified 96 Well Spheroid Plate along with Spheroid Formation Extracellular Matrix, leading to aggregation or spheroid formation of cells. (labcritics.com)
The results obtained in this study concluded that calvaria cells viability and hence proliferation increased when grown embedded within 0.25% Puramtrix while mesenchymal stem cells increased when embedded in 0.5% Puramatrix. (ncl.ac.uk)
The current investigation aimed to demonstrate how interpretation of results from several standard cytotoxicity and proliferation assays vary in dependence on contributions from the pentose phosphate pathway (PPP). (cdc.gov)
Non-tumorigenic Beas-2B cells were treated with graded concentrations of benzo[a]pyrene (B[a]P) for 24 and 48h prior to cytotoxicity and proliferation assessment with commonly used MTT, MTS, WST1, and Alamar Blue assays. (cdc.gov)

developed a high-throughput 3D spheroid-based screening assay to identify drug candidates that target RAS proteins. (promegaconnections.com)
The focus of the present study was to develop a high-throughput 3D neurite outgrowth assay using iPSCderived neurons developed in the microfluidic OrganoPlate ® platform, with the goal of establishing 3D models for neurodegenerative diseases and neurotoxicology screens 3 . (moleculardevices.com)

The cultures were exposed to cisplatin and cetuximab and the morphological changes of spheroids over time and the expression changes of target proteins were compared. (medsci.org)
Cellular transformation/tumorigenicity assays using cultures of cells in semi-solid media (soft agar or Matrigel) has been well established for cancer research 1,2,5 . (moleculardevices.com)
To prepare spheroid cultures in semi-solid media, human colon carcinoma HCT116 cells were pre-mixed with a solution of 4.25 mg/mL cold Matrigel (Corning) diluted in growth media and plated into 96-well half-area plates (Greiner) at a density of \~500 cells/well for a total volume of 25 μL. (moleculardevices.com)
The resulting spheroid cultures were treated with anti-cancer compounds for 5 days. (moleculardevices.com)
And due to lack of gas exchange there is an upper limit on the length of an assay as well-at least in aerobic cultures. (genengnews.com)
Cultures are subjected to a standard 2D viability assay to determine the health of the cells two days after treatment with the test compound. (genengnews.com)
Co-inhibition of IGF and CHK1 caused synergistic suppression of cell viability, cell survival and tumour growth in 2D cell culture, 3D spheroid cultures and in vivo. (nature.com)
We have developed a 3D (spheroid) functional assay to assess the sensitivity of two PARPis (niraparib and olaparib) in ascites-derived primary cell cultures (AsPCs) from HGSOC patients. (biomedcentral.com)
Audrey has experience with cultures and applications of primary and engineered immune cells as well as with high throughput 2D and 3D assays. (cellculturedish.com)
While iPSC-derived neurons show great promise for compound screening and disease modeling, use of three-dimensional (3D) cultures is emerging as a valid approach for neuronal cell based assay development. (moleculardevices.com)
This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay. (technologynetworks.com)
Cell viability was determined in two different 3D cell culture systems - the RAFT™ 3D Cell Culture System and classical spheroid cultures generated in ultra-low attachment plates. (technologynetworks.com)

In recent years, a spheroid model produced by three-dimensional (3D)-culture has attracted attention as a better experimental model that is closer to the physiological environment in vivo [ 2 ]. (medsci.org)
In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)
However, this method may not be optimal for the delivery of cell spheroids where the 3D structure, essential to maximize their in vivo effects, is lost by direct seeding. (biomedcentral.com)
In addition, in vivo serial transplantation assays, dye exclusion assays, and isolation via cell surface specific antigen profile methods are now used to identify CSCs. (biomedcentral.com)
Matrigel ® matrix, Transwell ® permeable supports and spheroid microplates are just a few Corning products being used to create more in vivo -like 3D environments used in cutting edge cancer research studies. (cellculturedish.com)
For in vivo studies, the murine sponge assay of angiogenesis was used as a model of breast cancer-associated vascularization. (uninsubria.it)
We have generated three-dimensional in vitro models of the human heart ('cardiac spheroids', CSs) by co-culturing human primary or iPSC-derived cardiomyocytes, endothelial cells and fibroblasts at ratios approximating those present in vivo. (ku.dk)
Our customer needed to obtain in-vivo like responses to drug treatment, through monitoring parameters such as gene expression, biomarker analysis, cellular viability, invasion capability. (dynamics365commerce.ms)

13] H. Wu, Y. Yang, P. O. Bagnaninchi, and J. Jia, â€œElectrical impedance tomography for real-time and label-free cellular viability assays of 3D tumour spheroids,â€ Analyst, vol. 143, no. 17, pp. 4189â€"4198, 2018. (sciencepubco.com)

Then, spheroid size and phenotype were characterized by volume, diameter, and fluorescence intensities of cellular markers. (moleculardevices.com)
Multi-parametric outputs were generated using a customized analysis module (Figure 3) and the readouts for each individual spheroid included measurements for nuclear count, size, volume, intensity, live cells per spheroid, whole cell volume and diameter, spheroid diameter, and average intensities of cellular markers (calcein AM, Phalloidin, or Hoechst). (moleculardevices.com)
Suppression of cellular network formation and disruption of spheroid development of breast cancer cells by Boswellia sacra essential oil suggest that the essential oil may be effective for advanced breast cancer. (biomedcentral.com)
Forming cellular spheroids in vitro can be achieved using various methods, including hanging drop, static suspension culture, encapsulation/entrapment, and low adherence multi-well. (ncl.ac.uk)
As noted by In Vitro Cellular & Developmental Biology - Animal , you may not want to rely on assays developed for 2D when moving into 3D data gathering. (corning.com)

The cell viability assay dataset for the main study contains drug sensitivity score (DSS) values for each of the tested drugs derived from the WTC spheroids of 45 BC patients. (scilifelab.se)

In the future, it may become possible to produce tumor spheroids from tissue samples of oral cancer patients, and then apply them to drug screening and to develop individualized diagnostic and treatment methods. (medsci.org)
Therefore, a few methods have been developed to use these characteristics in order to assay cancer stem cells. (biomedcentral.com)
The conventional methods of cryopreservation of islets use intact native islets, and the viability and function achieved after thawing with these methods is poor. (ku.edu)
Further details on the cell viability assay, as well as the DSS estimation are available in the Materials & Methods part of Chen et al. (scilifelab.se)
Methods used were metastatic invasion assay, gene expression analysis by RT-PCR on hypoxic response and oncopathway genes, and large-scale protein profiling to detect proteomic levels of metalloproteinases and surface proteins known to be involved in colorectal cancer invasion. (dynamics365commerce.ms)

Even though they are typically used for migration and invasion assays, they may find some use here. (cellculturedish.com)
It is also possible to combine Corning 96HTS inserts with the Corning spheroid microplate in order to look at immune cell migration/invasion towards the cancer organoid. (cellculturedish.com)
NCRC supported researchers at the Royal College of Surgeons in Ireland and colleagues at Johns Hopkins University, Texas Tech University, and Monash University have developed a 3D experimental model of neuroblastoma invasion and shown that the composition of the extracellular matrix influences the growth, viability, and local invasion of patient-derived neuroblastoma cells. (nationalchildrensresearchcentre.ie)
The composition of the ECM influenced the growth, migration, viability, and local invasion of the neuroblastoma organoids. (nationalchildrensresearchcentre.ie)
The combination of neuroblastoma organoids, real-time imaging, and the novel 3D culture assays developed in the study will enable rapid progress in elucidating the molecular mechanisms that control neuroblastoma invasion. (nationalchildrensresearchcentre.ie)
Analyses of the cell viability, cell cycle, migration, and matrigel invasion assays were performed to elucidate how INT 747 and /or FMD functions in prostate cancer. (edu.iq)
Interestingly, the combination treatment on PC-3 cells not only showed several lines of evidence of apoptotic cells death but also inhibited carcinogenic potential as evaluated by impairment of spheroid formation capacity and delayed wound healing and matrigel invasion. (edu.iq)
Role of PDL-1 in NSCLC chemoresistance was assessed using CRISPR/Cas9 knockdown and various functional assays including MTT, cell invasion, sphere formation, and cell apoptosis. (cdc.gov)

Up on formation of these spheroids, they are subjected to drug treatment and the viability of tumor cells is evaluated. (labcritics.com)

CellTiter-Blue Cell Viability Assay kit (Promega, Madison, WI) was used to determine the viability of the spheroids. (vittori-lab.com)
Caspase activities in the cells were determined using Caspase-Glo 3/7, Caspase-Glo and Caspase-Glo 9 Assay kits (Promega, Madison, WI) according to the manufacturer protocol. (vittori-lab.com)

The tumor spheroid expansion is visualized and quantitated using microscopy and image analysis techniques respectively. (labcritics.com)
It's also possible to use high throughput imagers via phase or fluorescent microscopy and image analysis software to quantify changes in spheroid size, metabolic activity and fluorescent staining. (cellculturedish.com)

The cell viability is assessed by using Resazurin and measuring the resulting fluorescence. (labcritics.com)
Tetrazolium reduction and resazurin assays are the mainstay of routine in vitro toxicity batteries. (cdc.gov)

The aim of this study was to compare the potential of human BMSC cultured as 2D monolayers or 3D spheroids encapsulated in constructs of 3D-printed poly-L-lactide-co-trimethylene carbonate scaffolds and modified human platelet lysate hydrogels (PLATMC-HPLG) for bone regeneration. (biomedcentral.com)
We performed comparative analysis for them, which involved developing two HT-29 colorectal cancer models: as monolayers in a conventional incubator and as 3D spheroids under physioxia, in the presence/absence of gamma-interferon. (dynamics365commerce.ms)

To prepare for an assay, the researcher fills each well of the plate with some biological entity that they wish to conduct the experiment upon, such as a protein, cells, or an animal embryo. (wikipedia.org)
The assay requires cells to grow in an anchorage independent manner, which is a hallmark of cancer cells. (moleculardevices.com)
In an alternative assay, cells were fixed for 1 hour using a 4% formaldehyde solution, washed 3x with PBS by removing half of the volume each time. (moleculardevices.com)
Spheroids and individual cells were defined based on size and intensity. (moleculardevices.com)
Because the assays require no labels they are noninvasive and nondestructive to the cells, inexpensive, simple, and quick to set up and use. (genengnews.com)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). (biomedcentral.com)
We found 37 differentially expressed miRNAs in the CD133 + spheroid-forming subpopulation of OVCAR3 cells, 34 of which were significantly up-regulated, including miR-205, miR-146a, miR-200a, miR-200b , and miR-3, and 3 of which were significantly down-regulated, including miR-1202 and miR-1181 . (biomedcentral.com)
In this study, miRNA expression profiles of a CD133 + spheroid-forming subpopulation of OVCAR3 ovarian cancer cells were investigated to identify miRNA expression profiles that contribute toward the characteristics of CSCs in ovarian cancer. (biomedcentral.com)
The cell layers in spheroids resemble close to that of an avascular tumor with proliferating cells layers on the spheroid surface, quiescent cells in the intermediate layers and necrotic cells at the core. (labcritics.com)
Next, the effect of creating hybrid islets/spheroids using mixtures of cells was tested by combining cells from different human donors, or by combining mesenchymal stem cells (MSCs) and islet cells. (ku.edu)
In order to minimize the need for assay repetition during the drug screening procedures, multi-donor Kanslets, consisting of islet cells from different donors, were created. (ku.edu)
these spheroids were highly viable even long-term in culture, but secreted less insulin irrespective of the proportion of MSCs and islet cells in the spheroids, when compared to the spheroids containing only islet cells. (ku.edu)
Cell viability was expressed as the percentage of the fluorescence in the treated cells relative to that of the controls. (vittori-lab.com)
Caspase Assays A549 cells treated with PCAIs (1 C 5 M) for 48 h were used to determine caspase activities and levels of caspase expression. (vittori-lab.com)
Finally, the effect of bortezomib + OTSSP167 combination was tested on MDA-MB-231 breast cancer cells, and it similarly determined that 20 nM bortezomib +40 nM OTSSP167 combination completely blocked the forma- tion of 3D spheroids. (mdm2-receptor.com)
This study aimed to explore the suitability of a 3D liver spheroid model of HepaRG cells to predict DIC in early drug development using high-content imaging (HCI). (vub.be)
Corning scientists have done a similar assay with NK92 cells and a lung cancer cell line. (cellculturedish.com)
Cell viability was determined on BT459 and MDA-MB-231 (i.e., breast cancer cells) and H9C2 (i.e., rat cardiomyocytes) cells, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (uninsubria.it)
A spheroids assay was used as a 3D in vitro model on BT459 and MDA-MB-231 cells. (uninsubria.it)
To assess neuronal viability, cells were stained live using a mixture of three dyes: the viability dye Calcein AM (1 µM), the mitochondria potential dye MitoTracker Orange (1 µM), and the Hoechst nuclear dye (2 µM). (moleculardevices.com)
One approach to deliver high numbers of cells to a defect site is as cell aggregates or spheroids. (ncl.ac.uk)
Both viability and ability of cells to differentiate was investigated. (ncl.ac.uk)
The MTT assay was used to assess cells viability while their ability to differentiate was assessed by measuring alkaline phosphatase activity as well as measuring gene osteogenic markers expression via qRT-PCR. (ncl.ac.uk)
Cx-43 iv was immunolocalised to gap junction structures in cells after osteogenic treatment on a flat substrate but this was more difficult to assess in the 3D spheroids. (ncl.ac.uk)
SaOS-2 cells are more likely to form loose cell aggregates (Fig. 1A) and the HDF cells form compact spheroids (Fig. 1B). (upmbiomedicals.com)

Maximum projection image of spheroids in Matrigel stained with Hoechst and AF488-phalloidin. (moleculardevices.com)
Z-planes of spheroids in Matrigel. (moleculardevices.com)
3D transfection is possible in Corning® Matrigel® matrix, as well as in Corning Ultra Low Attachment vessels and Corning spheroid microplates. (cellculturedish.com)

With a wide range of flexibility, we offer numerous off-the-shelf assays to clients for evaluating the efficacy and potential toxicity of cancer therapeutics. (visikol.com)
Since these assays are conducted in a well-plate format, they are amenable to larger screening campaigns to assess the efficacy of therapeutic strategies in ways not previously possible without animal studies. (visikol.com)

Cell seeding ensures uniform spheroid size and physiology, providing a robust and reproducible assay format for drug screening and pathway analysis. (labcritics.com)
Extensive analysis identified that the most efficient and reproducible method for the formation of spheroids using rat osteoblasts and human MSCs was through their culture at a specific concentration in polyHEMA coated plates. (ncl.ac.uk)

Confocal imaging and analysis protocols were optimized for assessing morphological phenotypes and viability of neurons cultured in a 3D matrix. (moleculardevices.com)

In 3D assays, neuroblastoma organoids displayed heterogenous morphologies with both non-invasive (cyst, spheroid) and invasive (collective mesenchymal, elongated, neuronal, protrusive) phenotypes identified. (nationalchildrensresearchcentre.ie)

A High-Throughput Three-Dimensional Cell Migration Assay for Toxicity Screening with Mobile Device-Based Macroscopic Image Analysis. (chemosen3d.com)

Typically, an integrated robot system consisting of one or more robots transports assay-microplates from station to station for sample and reagent addition, mixing, incubation, and finally readout or detection. (wikipedia.org)

Inhibitors (e.g. immune checkpoints, enzymatic assays, signalling pathway. (dynamics365commerce.ms)

8 days after seeding, the spheroids were exposed to nine half-log spaced concentrations per compound. (vub.be)
For the drug profiling assay, each compound covered five concentrations ranging from 10 μM to 1 nM (2 μM to 0.2 nM for trastuzumab and pertuzumab) in 10-fold dilutions and was dispensed using the acoustic liquid handling system Echo 550 (Labcyte Inc) to make spotted 384-well plates. (scilifelab.se)
Cell culture, compound treatment, and cell staining steps were performed using the microfluidics assay format. (moleculardevices.com)

Scientists have been working with very specific assays for very specific targets for years, explained Magnus Jansson, Ph.D., CSO of SymCel Sverige. (genengnews.com)
On each model, a viability assay was performed after 3 days incubation time with inhibitors, followed by large-scale proteome profiling analysis on 2000 targets from each cell lysates. (dynamics365commerce.ms)

Quercetin also has been studied by our scientific collaboration with M D Anderson, in 3 dimensional cell culture model and has shown inhibitory effect on cell migration and glioblastoma spheroid viability ( Figure 2 ). (scirp.org)
Hilary has extensive experience with cancer cell migration, immune oncology assays and 3D cell culture. (cellculturedish.com)

Apoptosis was assayed by genomic DNA fragmentation. (biomedcentral.com)
To test this, they used the RealTime-Glo™ Annexin V Apoptosis Assay . (promegaconnections.com)
This bioluminescent assay is able to detect apoptosis in real time, based on the exposure of phosphatidylserine on the outer leaflet of the cell membrane when apoptosis occurs. (promegaconnections.com)
Using this assay, they found that Proscillaridin A induced apoptosis at earlier time points and higher rates in 3D spheroids expressing oncogenic KRAS compared with wild-type KRAS. (promegaconnections.com)

3D assays were formerly labor-intensive and inconsistent due to the subjective definitions of colonies and, non-suitable for high-throughput screening. (moleculardevices.com)
The authors in this study wanted to test a new approach using a 3D spheroid-based screening assay to find drugs that target RAS proteins. (promegaconnections.com)
This study shows that high-throughput screening in 3D spheroids can identify potential drugs that would not have been discovered in a 2D format. (promegaconnections.com)
I am exploring some high-throughput options for screening spheroids. (cellculturedish.com)
There are numerous options for high-throughput screening spheroids. (cellculturedish.com)
Visikol specializes in assays for the screening of anti-cancer drugs. (visikol.com)
Utilizing High Content Screening, our assay formats provide the ability to simultaneously measure multiple endpoints. (visikol.com)
Advances in cell culture consumables mean that spheroid and organoid culture is now highly amenable to high content screening . (corning.com)

These include assays for immune cell infiltration into 3D tumor spheroids, as well as assaying the penetration and distribution of biologicals into tumor spheroids. (visikol.com)

The company has developed a calorimetry-based assay system that can monitor the total metabolic activity by measuring the heat flow in and out of a cell culture well in real time, down to the microwatt level. (genengnews.com)
This protocol analyzes the culture and growth of 3D CM and UM spheroids and their reaction to bleomycin (2.5 µg/mL) alone, electroporation (EP) (750 Volts/cm, 8 pulses, 100 µs, 5 Hz) alone, and ECT as a combination of EP and bleomycin. (liverpool.ac.uk)
2 Bioinformatics was used to select drugs for combination studies in 2D cell culture and 3D spheroid cell viability assays. (residencetimer.com)
Assembly of Hepatocyte Spheroids Using Magnetic 3D Cell Culture for CYP450 Inhibition/Induction. (chemosen3d.com)
5. Desai, P. K., Tseng, H. & Souza, G. R. Assembly of Hepatocyte Spheroids Using Magnetic 3D Cell Culture for CYP450 Inhibition/Induction. (chemosen3d.com)
This record is a collection of Whole-genome sequencing (WGS), RNA sequencing (RNA-seq), NanoString's nCounter® Breast Cancer 360 (BC360) Panel and cell viability assay data, generated as part of the study " Breast cancer patient-derived whole-tumor cell culture model for efficient drug profiling and treatment response prediction " by Chen et al. (scilifelab.se)
3D cell culture, including the increased use of spheroids and organoids, presents unique cell imaging problems, especially when creating a digital model is necessary for experimental analysis. (corning.com)
Spheroids and organoids are replacing standard cell culture in many traditional cell-based assays. (corning.com)
For example, U-bottom spheroid culture plates not only optimize growth conditions but also create uniform cell growth that sits centrally in each well. (corning.com)
HDF spheroids reached maximum size after 7 days culture, and subsequently the size decreased. (upmbiomedicals.com)

Cisplatin showed concentration-dependent antitumor effects due to loss of cell adhesion and spheroid disruption in each cell line, while cetuximab exhibited antitumor effects that correlated with EGFR expression in each cell line. (medsci.org)

Especially in drug discovery, most such assays have been geared toward a particular reaction-whether it is recognition of a cell surface marker, triggering endocytosis, or exciting or inhibiting a phosphorylation cascade. (genengnews.com)
Please explore our website to learn more about the assays we offer, and know that we offer custom drug discovery solutions to clients wishing to develop and utilize an assay more specific to their particular research objectives. (visikol.com)
These spheroids have been employed to investigate the dose-limiting cardiotoxicity of the common anti-cancer drug doxorubicin. (ku.dk)
Spheroids and organoids also offer a faster way to screen drug responses and study pathology compared to equivalent studies in lab species. (corning.com)

3D spheroid formation was facilitated with methylcellulose. (biomedcentral.com)
Cluster of differentiation (CD) 133 + and spheroid formation have been reported to be one of the main features of ovarian CSCs. (biomedcentral.com)

in which they normally form compact, viable spheroids) and used to determine the effect of the PCAIs. (vittori-lab.com)

Because of the distinct regulatory cues given by cell-cell contact in the spheroid, analysis was performed for connexin (Cx)-43, a gap junction protein and members of the ephrin/Eph family. (ncl.ac.uk)

For spheroids, tissue clearing is the only step. (corning.com)

The combination of the OMWW extracts with doxorubicin or 5-FU limited BT459 and MDA-MB-231 cell viability and the diameter of 3D spheroids, while mitigating their toxic effects on the rat H9C2 cardiomyocytes. (uninsubria.it)

HepaRG spheroids were cultured in 384-well ultra-low attachment plates. (vub.be)

Combined with miRNAs-based transcriptome sequencing assay, it was found that Tanshinone IIA downregulated miR-125b level and upregulated its target gene STARD13 (StAR-related lipid transfer protein 13) level, thus inactivating the miR-125b/STARD13 axis, which had been previously confirmed to promote breast cancer progression. (biomedcentral.com)
Boswellia sacra essential oil-mediated cell viability and death were studied in established human breast cancer cell lines (T47D, MCF7, MDA-MB-231) and an immortalized normal human breast cell line (MCF10-2A). (biomedcentral.com)
All three human breast cancer cell lines were sensitive to essential oil treatment with reduced cell viability and elevated cell death, whereas the immortalized normal human breast cell line was more resistant to essential oil treatment. (biomedcentral.com)

The effect on viability and growth of the 3D tumor spheroids was significant only after ECT. (liverpool.ac.uk)

Furthermore, the 3D spheroid model proved that bortezomib + OTSSP167 was more effective than the monotherapies as well as bortezomib + CVT-313 and bortezomib + BAPTA-AM combinations. (mdm2-receptor.com)
However, to maximize the cost savings and time efficiency, scientists must be aware of the factors that influence whether or not cell imaging returns high quality data, for both multiwell plate assays and for creating a digital model from z-stack layers. (corning.com)

An assay plate is simply a copy of a stock plate, created by pipetting a small amount of liquid (often measured in nanoliters) from the wells of a stock plate to the corresponding wells of a completely empty plate. (wikipedia.org)
SymCel calScreener sample vials in close up: calScreener offers calorimetry-based cell assays in a microtiter plate format. (genengnews.com)

Protein concentration was measured using a Pierce BCA protein quantification assay kit, according to the manufacturers protocol (Thermo Scientific, Waltham, MA). (vittori-lab.com)

Say you have a G-protein coupled receptor (GPCR) of unknown function-with this assay you can determine whether it effects a stimulatory or inhibitory response, or induces necrosis under your experimental conditions, Dr. Jansson proposed as an example. (genengnews.com)
The assay used to determine the spheroid viability was conducted 3-7 days following treatment. (liverpool.ac.uk)
Next, the authors wanted to determine how Proscillaridin A impacts tumor cell viability. (promegaconnections.com)

Anatomy18

Organisms6

Diseases17

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes46

Disciplines and Occupations1

Technology, Industry, Agriculture6

Information Science2

Health Care1

Monolayer7

Cytotoxicity assays2

Multicellular3

Luciferase reporter assay2

CellTiter-Glo2

Vitro assays2

Proliferation5

High-throughput 3D2

Cultures12

Vivo8

Tumour1

Cellular5

Dataset1

Methods5

Invasion8

Tumor cells1

Promega2

Microscopy and image analysis2

Resazurin2

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Cells26

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Efficacy2

Reproducible2

Morphological1

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Toxicity1

Reagent1

Pathway1

Compound3

Targets2

Migration2

Apoptosis4

Screening8

Immune cell1

Culture10

Disruption1

Drug4

Formation2

Viable1

Analysis was performed1

Tissue1

Diameter1

Ultra-low attachment plates1

Breast cancer3

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Model2

Plate2

Concentration1

Determine3