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  • Receptors
  • Retromer is a complex of proteins that has been shown to be important in recycling transmembrane receptors from endosomes to the trans -Golgi network (TGN). (wikipedia.org)
  • The neurotransmitters then diffuse across the cleft and bind to ligand gated ion channels and G-protein coupled receptors on the postsynaptic membrane. (wikipedia.org)
  • Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin. (nih.gov)
  • 2005), could cooperate with the SH2-like domain to enhance clustering of RTK-GIV complexes at the PM. We propose that the SH2-like domain is key to achieve targeting and proximity of GIV to RTKs, which allows GIV to integrate incoming signals from these receptors and modulate them via activation of G proteins in the vicinity of the receptors. (nih.gov)
  • Examples of such interdomain collaborations were reported in the case of other multimodular signal transducers that bridge RTKs and small G proteins, such as Ras-GAP (Schlessinger and Lemmon, 2003), which inactivates Ras in the vicinity of ligand-activated receptors. (nih.gov)
  • The ESCRT (endosomal sorting complex required for transport) machinery functions to sort cellular receptors into the lumen of the multivesicular body (MVB) prior to lysosomal degradation. (nih.gov)
  • Golgi reassembly stacking protein 65 (GRASP65), an NEM-sensitive membrane-bound component, is required for the stacking process.Temporal analysis suggests that p115 plays a transient role in stacking that may be upstream of GRASP65-mediated stacking.These results implicate p115 and its receptors in the initial alignment and docking of single cisternae that may be an important prerequisite for stack formation. (nih.gov)
  • A cell-free system that reconstructs these events has revealed that cisternal regrowth requires interplay between soluble factors and soluble N-ethylmaleimide (NEM)-sensitive fusion protein (NSF) attachment protein receptors (SNAREs) via two intersecting pathways controlled by the ATPases, p97 and NSF. (nih.gov)
  • NSF-mediated cisternal regrowth requires a vesicle tethering protein, p115, which we now show operates through its two Golgi receptors, GM130 and giantin. (nih.gov)
  • Fusion
  • A) The ability of the CISK PX domain to bind lipids was tested by a protein/lipid overlay assay using GST-CISK PX domain fusion proteins and nitrocellulose-immobilized phospholipid strips (100 pmole/spot). (nih.gov)
  • The expression of the GST fusion proteins is shown on the right. (nih.gov)
  • To further determine the relative preference of the CISK PX domain to various phosphoinositides, wild-type and mutant (R90A) glutathione S-transferase (GST)-PX fusion proteins were used to probe arrays containing different concentrations of phospholipids. (nih.gov)
  • In zebrafish, a BH3-only SNARE protein, BNip1, induces photoreceptor (PR) apoptosis in response to vesicular fusion defects (Nishiwaki et al. (arvojournals.org)
  • In zebrafish photoreceptors, β-SNAP regulates vesicular fusion process. (arvojournals.org)
  • Membrane Protein
  • The membrane protein alkaline phosphatase is delivered to the vacuole by a route that is distinct from the VPS-dependent pathway. (nih.gov)
  • In this study we demonstrate that, whereas VPS45 and VPS27 are required for the vacuolar delivery of several membrane proteins, the vacuolar membrane protein alkaline phosphatase (ALP) reaches its final destination without the function of these two genes. (nih.gov)
  • Chemistry
  • We have a strong interest in new lab members who bring backgrounds in protein biochemistry, organic chemistry, biophysics, and bio-engineering. (yale.edu)
  • Mechanisms
  • A second area of research uses transgenic and gene knockout mouse models to study proteins involved in GLUT4 targeting, to determine if insulin acts through similar mechanisms in fat and in muscle, and to test the importance of these pathways for overall glucose homeostasis. (yale.edu)
  • Additionally it has been proposed that the directed transport of active signaling complexes to the nucleus might be required to enable signaling as random diffusion is too slow and mechanisms permanently downregulating incoming signals are strong enough to shut down signaling completely without additional signal-transducing mechanisms. (wikipedia.org)
  • cytoplasm
  • Recent data shows that the glutamatergic active zone does contain the dense protein material projections but these projections were not in a regular array and contained long filaments projecting about 80 nm into the cytoplasm. (wikipedia.org)
  • interaction
  • PDZ domains are protein:protein interaction domains that serve as molecular scaffolds, especially in the case of specialized cellular environments like polarized airway epithelial cells of the human lung. (dartmouth.edu)
  • major
  • Using these methods they have demonstrated that defects in insulin stimulated glucose transport and muscle glycogen synthesis are the major factors responsible for insulin resistance. (yale.edu)
  • regulator
  • This gene provides instructions for making a protein known as the lysosomal trafficking regulator. (wikipedia.org)
  • Next, we decreased the level of protein transport through the connecting cilium to the outer segment in the β-SNAP mutant by injection of morpholino- antisense oligos for intra-flagella transport regulator, IFT88 and Kif3b, and examined whether PR apoptosis was inhibited at 3.5 dpf. (arvojournals.org)
  • pathways
  • CaMK1-α and P90RSK) were mainly involved in ErbB and neurotrophin signaling pathways as determined using DAVID software, while 25 down-regulated proteins (e.g. (cancerindex.org)
  • Annotation
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • formation
  • Elevated cysteinyl adducts to l -DOPA and DOPAC are seen early and are followed by increased striatal protein carbonyl and 3-nitrotyrosine formation. (jneurosci.org)
  • MARS: A protein family involved in the formation of vertical skeletal elements. (semanticscholar.org)