• Dr. Jale Özdemir offers original research regarding the cytotoxic impacts of calcium hydroxide and tricalcium phosphate, which are used in endodontics, as examined in VERO cell cultures (African Green Monkey Kidney Cell). (dentistryiq.com)
  • Cytotoxic impacts of calcium hydroxide and tricalcium phosphate, which are used in endodontics, have been examined in cell cultures. (dentistryiq.com)
  • 3 Material and methods Cytotoxic impacts of calcium hydroxide and tricalcium phosphate have been examined in Vero cell culture as in vitro in our study. (dentistryiq.com)
  • Assessment of cytotoxic impact Vero (African Green Monkey Kidney Cell), produced with DMEM (Biochrom KG - DMEM), which contains 10% FCS (Fetal Calf Serum, Biochrom KG, Berlin), has been used as a cell culture in this research. (dentistryiq.com)
  • The 50% cytotoxic dose in Vero cells was 3.4 or 1.7 pg (depending on the assay conditions), about 3-5 times higher than the archetypical Stx2a, while the activity of Stx2f and Stx2a in a cell-free rabbit reticulocyte system was similar. (usda.gov)
  • Moreover, In-vitro cytotoxic effects and the antibacterial activity of Mn 3 O 4 NPs showed a better result at the concentration of 50 µg/100µl in Vero cell and the Zone of inhibition was measured for Enterobacter . (sciepub.com)
  • The aim of this study was to investigate the possible time- and dose-dependent cytotoxic effects of cobalt chloride on Vero cells. (hacettepe.edu.tr)
  • CoCl2 had dose- and time-dependent cytotoxic effects in Vero cells. (hacettepe.edu.tr)
  • In terms of cytotoxicity on the African monkey kidney epithelial (Vero) cells, the chloroform extract of P. odorata produced the lowest 50% cytotoxic concentration (100.3 ± 4.2 μg/mL). (um.edu.my)
  • The cytotoxic effect of the aqueous and ethanol extracts of V. mespilifolia, K. africana and their combination were also evaluated using HeLa cells. (ru.ac.za)
  • According to the American National Cancer Institute, crude plant extracts are considered cytotoxic in an in vitro assay when concentrations 20 μg/mL and below produce 50% inhibition of tumor cells, after an exposure time of 48 hours. (ru.ac.za)
  • Α-mangostin, β-mangostin, mangaxanthone B and mangaphenone showed significant cytotoxicity through MTT assay against MCF-7 and MDA-MB-231 cells with IC50 values ranging from 4.4 to 12 µM. (wikipedia.org)
  • VERO (African Green Monkey Kidney Cell) has been used as cell culture for the cytotoxicity assessment of calcium hydroxide and tricalcium phosphate. (dentistryiq.com)
  • The P. harmala extract was tested for its cytotoxicity effect on a Vero cell line. (ophrp.org)
  • The cytotoxicity studies on the Vero cell line (African green monkey kidney cell line) were studied by using MTT assay at 72 hrs. (sciepub.com)
  • The cultured cells were incubated with different concentrations of cobalt chloride ranging from 0.5 to 1,000 mu M, and cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and resazurin assays. (hacettepe.edu.tr)
  • Dose-dependent cytotoxicity was noted following exposure of cells to a parts per thousand yen250 mu M of CoCl2 for 24 h and a parts per thousand yen100 mu M concentrations of CoCl2 for 48-96 h. (hacettepe.edu.tr)
  • Pretreatment of cells with ZnCl2 for 4 or 24 h provided significant protection against cobalt chloride-induced cytotoxicity when measured with MTT assay. (hacettepe.edu.tr)
  • Preventive effect of ZnCl2 against CoCl2-induced cytotoxicity should be considered in detail to define exact mechanism of toxicity in Vero cells. (hacettepe.edu.tr)
  • Cytotoxicity of extracts was determined against Vero kidney cells using a colorimetric tetrazolium-based assay. (biomedcentral.com)
  • Using an antiviral assay based on quantitative reverse transcription-polymerase chain reaction (qRT-PCR), we found that bLf reduced progeny virus yield by up to ∼84,6% in African green monkey kidney epithelial cells (Vero E6) and ∼68,6% in adenocarcinomic human alveolar basal epithelial cells (A549) at 1 mg/mL, a concentration previously shown to have low cytotoxicity. (biorxiv.org)
  • Severson monitors cytotoxicity through several assays, including the CellTiter-Glo luminescent cell viability assay from Promega , Madison, Wis. (drugdiscoverytrends.com)
  • In vitro cytotoxicity against the HeLa S3 cell line has been evaluated by studying the inhibited growth rate [ 922 ], and some C 60 derivatives have shown promise as anti-cancer agents [ 1090 - 1092 ]. (nanomedicine.com)
  • On 9 and 10 March 2021, a workshop aimed at facilitating the implementation of replacement in vitro toxicity and antigenicity assays for Clostridium septicum vaccine antigens was jointly organised by the European Directorate for the Quality of Medicines & HealthCare (EDQM/Council of Europe), the European Partnership for Alternative Approaches to Animal Testing (EPAA) and the European Commission's Joint Research Centre (JRC). (edqm.eu)
  • One of these compounds (ebselen) also exhibited promising antiviral activity in cell-based assays. (nature.com)
  • Stx2f was found to be less toxic in cell-based assays than wild-type Stx2 (Sx2a) but more resistant to acid and heat treatments. (usda.gov)
  • This different receptor preference may also explain why Stx2f is less toxic in the cell-based assays we conducted. (usda.gov)
  • Stx2f was shown to be active in both cell-free and cell-based assays. (usda.gov)
  • The results of the study demonstrate the potential of bioelectric profiling as a tool for developing novel toxicity assays and associated biosensors with superior analytical capacity, speed of assay and the ability to respond to a broad spectrum of toxicants, at the same time satisfying the demand for increased cost-efficiency and ease of operation. (siftdesk.org)
  • Mammalian cells are a unique tool for in vitro toxicity assays, since they represent, with a fairly high degree of accuracy, the actual biological targets of many environmental pollutants or other compounds with proven or suspected toxicity. (siftdesk.org)
  • In a broader sense, higher eukaryotic cells have been recruited as biorecognition elements either in advanced cell-based biosensors (CBBs) or in cell-based assays (CBAs) [1]. (siftdesk.org)
  • Cell-based assays have become a popular platform for drug discovery. (drugdiscoverytrends.com)
  • Piazza is collaborating with Blueshift Biotechnologies , Sunnyvale, Calif., a vendor of instrumentation and reagents for cell-based assays and gene expression studies. (drugdiscoverytrends.com)
  • Quantum dots, a recent addition to the toolbox for cell-based assays, are nanometer-sized (about as large as a protein) semiconductor structures that fluoresce when exposed to broad-spectrum light. (drugdiscoverytrends.com)
  • The percentage viability of the cell line was carried out by using the MTT assay (Sigma, United States). (ophrp.org)
  • A gradual decrease in cell proliferation was observed at concentrations similar to a parts per thousand yen200 mu M in incubation periods of 24, 48, 72, and 96 h with MTT assay. (hacettepe.edu.tr)
  • The MOH fraction was innocuous against the human keratinocyte (HaCaT), macrophage (J77A.1), and Vero cell lines when assessed by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. (um.edu.my)
  • A clinical isolate hCoV-19/Italy/UniSR1/2020 (GISAID accession ID: EPI_ISL_413489) was isolated and propagated in Vero E6 cells, and viral titer was determined by 50% tissue culture infective dose (TCID 50 ) and plaque assay for confirming the obtained titer. (frontiersin.org)
  • Bioelectric assay methods based on mammalian cells have gained considerably in popularity over the past few years. (siftdesk.org)
  • In a typical assay, test cells are cultured for 24 hours into 384-microtiter plates pre-coated with library compounds, followed by a three-day virus infection and end point determination. (drugdiscoverytrends.com)
  • Severson's SARS assay, which uses Vero-E6 (monkey kidney) cells, has thus far uncovered several hits from a 100,000-compound library. (drugdiscoverytrends.com)
  • MTT Assay The SARS-CoV-2 Omicron Variant (BA.1) will be propagated in Vero cells and stored in -80 °C until further usage. (mbios.org)
  • All venoms were capable of reducing cell viability of all tested lineages. (edu.pe)
  • The Cell viability which was observed during the process depends upon the time exposure and concentration. (sciepub.com)
  • AVM induced the release of cleaved PARP and cleaved caspase‑3 caused apoptosis and inhibited the viability of these cells. (spandidos-publications.com)
  • In previous research, the authors demonstrated that the methanol extract of A. vulgaris (AVM) has the ability to inhibit the viability of CML cells ( 10 ). (spandidos-publications.com)
  • It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines (Figure 1) . (thermofisher.com)
  • which monitors cell viability as measured by ATP levels (dead cells don't have it). (drugdiscoverytrends.com)
  • The entry of propidium iodide caused by CPE was well correlated with the loss of cell viability monitored by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. (hal.science)
  • Firstly, treatment with Antrodia Camphorata mushroom extract will cause a reduction in the viability of the Sars-CoV-2 infected Normal Human Bronchial Epithelial (NHBE) cells. (mbios.org)
  • Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. (thermofisher.com)
  • Each reagent was used to transfect HeLa cells in a 96-well format at the indicated doses with an emerald green fluorescent protein (GFP)-expressing vector. (thermofisher.com)
  • Notably, gene expression changes after OC43 infection of Vero E6 or A549 cells were effectively reverted upon treatment with SBI-5844 or SBI-0498. (janelia.org)
  • A sufficiently high speed of measurement, low cost and ease-of-use, to satisfy high throughput requirements: most currently available methods for measuring toxic effects on mammalian and other cell types are associated with either optical (dye-based) [2] or electric (impedance spectrometry) [3] working principles implemented in multi-step processes, which demand several hours before enough data are collected. (siftdesk.org)
  • In contrast, none of the water extracts from the studied plants caused significant toxicity on the cells. (um.edu.my)
  • In another study, it was discovered that the active extracts in Antrodia camphorata notably reduced the viral replication when added to Vero-cells infected with Herpes-Simplex Virus (HSV) (He et al . (mbios.org)
  • The brine shrimp toxicity test revealed that all the three extracts of V. mespilifolia, aqueous and ethanol extracts of K. africana and aqueous and acetone extracts of the combination of both plants were toxic in relative to Meyer's index and other indices of toxicity. (ru.ac.za)
  • Abrin, like the similar plant toxin ricin, causes toxicity by inhibiting the formation (synthesis) of proteins in the cells of the exposed individual. (cdc.gov)
  • Micro-titration tablets have been left for incubation in the incubator, which contained 5% CO 2 at 37 C°. Toxic impacts which test materials caused changes in cell culture, which was covered as monolayer was assessed at 12-hour intervals via a tissue culture microscope (Olympus, Tokyo). (dentistryiq.com)
  • An aliquot (0.8 mL) of the transport medium of the nasopharyngeal swab (COPAN's kit UTM® universal viral transport medium-COPAN) of a mildly symptomatic SARS-CoV-2 infected patient was mixed with an equal volume of DMEM without FBS and supplemented with double concentration of P/S and Amphotericin B. The mixture was added to 80% confluent Vero E6 cells monolayer seeded into a 25 cm 2 tissue culture flask. (frontiersin.org)
  • ATCC) cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with non-essential amino acids (NEAA, 1x), penicillin/streptomycin (P/S, 100 U/mL), HEPES buffer (10 mM) and 10% (v/v) Fetal bovine serum (FBS). (frontiersin.org)
  • Pure filtrate (100% concentration) has been used as a positive check (sample check) and DMEM, which contained 2% FCS, has been used a negative check (cell check). (dentistryiq.com)
  • However, more extreme conditions (Ca 2+ was nominally absent and phosphate concentration raised to 25 mM as in the organ preservation solution University of Wisconsin solution) also increased cold-induced injury in rat hepatocytes and porcine aortic endothelial cells. (biomedcentral.com)
  • A biphasic survival profile of cells was observed at 1-25 mu M concentration range following 96 h of incubation. (hacettepe.edu.tr)
  • In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximized only when administered before and after the infection of Vero E6 and Caco-2 cells. (frontiersin.org)
  • Following this, the cells were treated with different concentrations of P. harmala ethanolic extract (1-100 μg/mL). (ophrp.org)
  • Further, the acute toxicity study on rats indicates treatment with MOH fraction even at a high dose of 5000 mg/kg body weight did not cause mortality. (um.edu.my)
  • This superior transfection performance allows you to reduce the reagent dose, thus helping lower toxicity risk when transfecting your cell line. (thermofisher.com)
  • He has thus far screened a library of one million compounds against bird flu for a "big pharma company" looking for both single-dose compound hits and dose-response toxicity. (drugdiscoverytrends.com)
  • Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner. (bvsalud.org)
  • In modern biotechnology, there is a need for pausing cell lines by cold storage to adapt large-scale cell cultures to the variable demand for their products. (biomedcentral.com)
  • In modern biotechnology and drug design large-scale cell cultures are necessary tools for the production of diverse recombinant proteins, such as Herceptin™, Enbrel™ or vaccines against the influenza virus strains H5N1 and H1N1 like Celvapan™ [ 1 - 7 ]. (biomedcentral.com)
  • Protein demand and, thus, the demand for cell cultures in protein production fluctuate. (biomedcentral.com)
  • This would allow a more flexible handling of cell cultures adapted to the demand, e.g. a rapid upscaling of cultures after storage, i.e. keeping cells in "stand-by" storage. (biomedcentral.com)
  • i.e. concentrations as in RPMI 1640) evoked a cell injury and loss of metabolic function corresponding to that observed in RPMI 1640. (biomedcentral.com)
  • Similar Ca 2+ and phosphate concentrations did not increase cold-induced cell injury in the kidney cell line LLC-PK 1 , porcine aortic endothelial cells or rat hepatocytes. (biomedcentral.com)
  • These data suggest that the combination of low calcium and high phosphate concentrations in the presence of glucose enhances cold-induced, iron-dependent injury drastically in Vero-B4 cells, and that a tendency for this pathomechanism also exists in other cell types. (biomedcentral.com)
  • Stx2j carrying strain, 2012C-4221, was induced when grown with sub-inhibitory concentrations of ciprofloxacin, and toxicity was detected. (cdc.gov)
  • We present a novel approach for assessing pesticide toxicity on mammalian cells, by measuring the response patterns of four cell lines (two neuroblastoma and two fibroblast) to three different pesticide groups (carbamates, organophosphates, pyrethroids) at a broad range of concentrations. (siftdesk.org)
  • 0.1 ml Vero cell suspension, which contains 3 x 10 5 cells in each milliliter of 96 celled micro-titration tablet (Falcon, Oxnard, Calif.), has been placed and incubated in the incubator, which contained 5% CO 2 at 37 C°. The methodology chosen by Aydın et al. (dentistryiq.com)
  • Two different aspects of the cellular bioelectric response are measured, namely the potential and the combined resistance + capacitance of the cell suspension. (siftdesk.org)
  • The water extract of A. nidus warrants further investigation since it showed the strongest fungicidal activity and the highest total activity (179.22 L/g) against Issatchenkia orientalis, and did not cause any toxicity to the Vero cells. (um.edu.my)
  • In previous research, the authors demonstrated that the methanol extract of Artemisia vulgaris (AVM) has the ability to inhibit chronic myeloid leukemia (CML) cell proliferation. (spandidos-publications.com)
  • This serves as the control as well as a method to evaluate the toxicity of the extract. (mbios.org)
  • CPE is the major virulence factor of this bacterium and acts on the claudin-receptor containing tight junctions between epithelial cells resulting in various gastrointestinal diseases. (hal.science)
  • In addition to this iron-dependent pathway, other changes in cellular ion homeostasis have also been described to contribute to cold-induced cell injury. (biomedcentral.com)
  • Classically, a cellular accumulation of sodium due to a reduced Na + /K + -ATPase activity resulting in cell swelling was thought to cause cold-induced cell injury [ 19 , 20 ]. (biomedcentral.com)
  • Recent studies were pioneered in host purine metabolic pathway, a conserved process responsible for providing host cells with a ready supply of guanosine triphosphate (GTP) for critical cellular processes [ 9 ]. (biomedcentral.com)
  • Both the cell line and the mode of measurement affected the observed in vitro cellular responses, with each cell type providing a unique pattern. (siftdesk.org)
  • Whichever the case, the final output of any cell-based test system is the measurement of the deviation of a predetermined cellular function from control, "baseline" levels as a result of exposure to a toxic compound. (siftdesk.org)
  • Morphology changes, rounding and grouping of cells, have been taken into account during toxicity assessment. (dentistryiq.com)
  • These SP constitute an important ingredient of cell walls and get harvested by suitable extraction or precipitation method, followed by purification, characterization and biological studies (Fig. 2 ). (springer.com)
  • Conclusions: Here, we detail the purification, biochemical properties, and toxicity of Stx2f, from an E. coli strain isolated from a feral pigeon. (usda.gov)
  • AuNPs interact with cell wall macromolecules, damaging them and affecting membrane proteins [ 9 ] . (encyclopedia.pub)
  • Because quantum dots are approximately the size of proteins, cells process them like molecules, not particles. (drugdiscoverytrends.com)
  • Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment. (bvsalud.org)
  • We compared various cell culture media/solutions for cold storage of Vero-B4 kidney cells, a cell line widely used in biotechnology. (biomedcentral.com)
  • Calu-3 (Human lung cancer cell line, ATCC HTB-55) were cultured in MEM supplemented with NEAA (1x), P/S (100 U/mL), HEPES buffer (10 mM), and 10% (v/v) FBS. (frontiersin.org)
  • We were very happy and surprised to see Lipofectamine® 3000 reagent provide a more than 10-fold difference in transfection efficiency in our difficult-to-transfect cell line. (thermofisher.com)
  • Chinese hamster ovary (CHO) is one of the most widely used non-human mammalian cell lines. (thermofisher.com)
  • Lipofectamine 3000 Transfection Reagent delivers a high efficiency for hard-to-transfect cell lines. (thermofisher.com)
  • When performing transfections in hard-to-transfect cell lines, Lipofectamine 3000 reagent can be an ideal choice. (thermofisher.com)
  • Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. (thermofisher.com)
  • The Lipofectamine 3000 transfection protocol was developed to be easy to use, while still ensuring optimum performance and reliability in a wide panel of cell lines. (thermofisher.com)
  • The easy protocol, exceptional plasmid DNA delivery, and the ability to effectively generate stable cell lines makes Lipofectamine 3000 reagent, the perfect reagent for CHO cell transfection experiments. (thermofisher.com)
  • Still, the vast number of pollutants of interest has prohibited the rapid development of cell lines with selective biorecognition elements. (siftdesk.org)
  • 4. Human Abortion Cells (MRC5) - unethical procedures are used to manufacture vaccines using cells derived from human fetuses aborted decades ago. (vaccineinjurynews.com)
  • As a result, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 (human wild‑type) or TCCY‑T315I (human imatinib‑resistant) and the Ba/F3‑(T315I/E279K/Y253H) (mouse BCR/ABL point mutation‑transfected cells). (spandidos-publications.com)
  • Thus, cells expressing different forms of BCR/ABL were recruited for the present study, including K562 [human wild-type (WT)] or TCCY-T315I [human imatinib-resistant (IR)] and the Ba/F3-(T315I/E279K/Y253H) (mouse BCR/ABL point mutation-tranfected cells). (spandidos-publications.com)
  • Caco-2 (Human epithelial colorectal adenocarcinoma cells, ATCC HTB-37) cells were cultured in Minimum Essential Medium (MEM) supplemented with NEAA (1x), P/S (100 U/mL), HEPES buffer (10 mM), sodium pyruvate (1 mM), and 20% (v/v) FBS. (frontiersin.org)
  • the company's anti-HIV drugs apparently have about one-tenth the toxicity of current HIV cocktails, and human trials start in 2003 [ 259 ]. (nanomedicine.com)
  • Normal Human Bronchial Epithelial (NHBE) cells will be cultured and maintained. (mbios.org)
  • Vu can then observe nerve cells internalizing the quantum dot-protein conjugate, and watch the conjugate travel through the cell to high-growth areas. (drugdiscoverytrends.com)
  • found that ivermectin has shown in vitro activity against SARS-COV-2 (COVID-19) in tissue culture with Vero-hSLAM cells. (mectizan.org)
  • This will be done to infect the NHBE cells with the SARS-CoV-2 Omicron Variant (BA.1). (mbios.org)
  • The inhibition of cell wall β-glucan synthase leads to changes in the cell wall integrity and further cell damage [ 9 ] [ 10 ] . (encyclopedia.pub)
  • Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)
  • by inhibiting the production of virulence factors such as exopolysaccharides and metabolic activity such as surface hydrophobicity playing an important role in bacterium-host cell interactions and biofilm architecture in microbes, respectively [ 7 ] . (encyclopedia.pub)
  • The activity of CPE on Vero cells was demonstrated by the entry of propidium iodide (PI) in the cells. (hal.science)
  • Subsequent work [ 5875 ] by this research group found that the antibacterial action of carboxyfullerene on Gram-positive bacteria is achieved by insertion into the cell wall and destruction of membrane integrity. (nanomedicine.com)
  • Cold storage in RPMI 1640 medium, a recommended cell culture medium for Vero-B4 cells, surprisingly, strongly enhanced cold-induced cell injury in these cells in comparison to cold storage in Krebs-Henseleit buffer or other cell culture media (DMEM, L-15 and M199). (biomedcentral.com)
  • Cells may be "drugged" after culture as well. (drugdiscoverytrends.com)
  • Now distributed in a pandemic pattern, this disease still lacks an effective drug treatment with low toxicity, leading pharmaceutical companies and research labs to work against time to find a candidate molecule to efficiently treat the affected patients. (biorxiv.org)
  • High doses in animal models have produced serious toxicity. (mectizan.org)
  • As IMPDH inhibitors targeted on host cells, it would be less susceptible for selection of drug-resistant strains. (biomedcentral.com)
  • In addition, a significant point is the belonging of potentially destroyed bacteria to Gram+ or Gram−, according to their cell walls structural features. (encyclopedia.pub)
  • Although Gram-positive and Gram-negative cell walls are negatively charged with a high-affinity degree to positively charged AuNPs, having a thinner cell wall, Gram-negative bacteria are more simply exposed to AuNPs, while Gram-positive have rigid peptidoglycan layers on their surface, which prevent the AuNPs entry. (encyclopedia.pub)