Vectors pseudotyped. Medical search. Frequent questions

Several virus types have been studied for use as viral vectors in gene therapy, including adenovirus, lentivirus, retrovirus, and adeno-associated virus (AAV). (biopharminternational.com)
It is important to note that measuring the functional titer of a recombinant lentivirus is not only dependent on how efficiently the transfer vector is packaged but also the transduction efficiency of the cell line used. (sigmaaldrich.com)
In addition, lentivirus particles pseudotyped with SARS-CoV-2 Omicron variant S were used to perform neutralization assays. (news-medical.net)
A three-vector lentivirus system provides space for delivering gene-based treatment into cells by moving the gagpol viral packaging genes onto a separate vector. (promegaconnections.com)

It has been previously demonstrated that the use of replication-defective viruses, such as lentiviral vectors pseudotyped with influenza A hemagglutinins in microneutralization assays (pMN) is a safe and sensitive alternative to study antibody responses elicited by natural influenza infection or vaccination. (ox.ac.uk)
Other studies have used SARS-CoV S pseudotyped viruses for identifying receptors ( 24 ), examining viral tropism ( 25 - 27 ), and measuring neutralizing antibody responses ( 18 , 28 - 30 ). (cdc.gov)
Crucially, some viral vectors (i.e., viruses specifically used to deliver genetic material into cells) have the potential to circumvent the blood-brain- (BBB) and blood-spinal cord barriers (BSCB) when intravenously injected. (frontiersin.org)
Such chimeras are termed pseudotyped viruses . (genetherapynet.com)
Viral vector systems are gutted viruses where the packaging signals (e.g., the genetic signals that target the wrapping into the viral particles) are fused to the genes of interest (the payload), and the remaining necessary elements are placed on separate genetic elements, often three or more, says Carsten Carstens, senior scientist, R&D, Agilent Technologies. (biopharminternational.com)
Laboratory-made viruses - viral vectors - retain the natural capability of wild-type viruses of delivering genetic material to the cells that they infect and are therefore an ideal vehicle to transport genes to sick cells. (themedicinemaker.com)
Lentiviral vectors are enveloped viruses, which means that they bud from the producer cell membrane and are released into the cell culture medium. (themedicinemaker.com)
Their outer layer can be "pseudotyped" - meaning that the original surface proteins can be replaced with those of other viruses to direct tropism and reach a high specificity for the target cells. (themedicinemaker.com)
Design and order pseudotyped viruses for your research at COVID-19 Coronavirus Solutions . (vectorbuilder.kr)

In this study, baculovirus pseudotyped with vesicular stomatitis virus glycoprotein was used as a vector to express the hemagglutinin (HA) protein of highly pathogenic H5N1 avian influenza virus, A/Chicken/Hubei/327/2004 (HB/327). (unboundmedicine.com)
We analyzed here, the transduction efficiency of a pDC cell line, GEN2.2, and of pDC derived from CD34+ progenitors, using lentiviral vectors (LV) pseudotyped with different envelope glycoproteins such as the vesicular stomatitis virus envelope (VSVG), the gibbon ape leukaemia virus envelope (GaLV) or the feline endogenous virus envelope (RD114). (biomedcentral.com)
Put another way, when the cells requiring modification are the only ones that come into contact with the vectors, no specific viral tropism is required, meaning that the vectors can carry broad-tropism envelope proteins, such as the vesicular stomatitis virus envelope protein G (VSG-G). (themedicinemaker.com)
Por ejemplo, un vector viral con VIRUS DE LA INMUNODEFICIENCIA DEL SIMIO puede recubrirse con PROTEÍNAS DE LA ENVOLTURA VIRAL del virus de la estomatitis vesicular. (bvsalud.org)
For instance a viral vector with SIMIAN IMMUNODEFICIENCY VIRUS can be coated with the VIRAL ENVELOPE PROTEINS from Vesicular stomatitus virus. (bvsalud.org)

We sought to develop novel rat and nonhuman primate (NHP) models of MSA by overexpressing α-syn in oligodendroglia using a novel oligotrophic adeno-associated virus (AAV) vector, Olig001. (biomedcentral.com)

Evaluation of CD46 re-targeted adenoviral vectors for clinical ovarian cancer intraperitoneal therapy. (cardiff.ac.uk)

The efficiency of packaging a delivery construct, also known as the transfer vector, into lentiviral particles can vary greatly depending on the size and composition of the transfer vector. (sigmaaldrich.com)
Thus, a reasonably well packaged, VSV-G pseudotyped lentiviral vector will have an infectivity in the range of 1 TU per 200 viral particles for inefficient transfer vectors to 1 TU per 1 viral particle when 100% efficient viral packaging occurs. (sigmaaldrich.com)
All non-tagged spike protein expression vectors have been functionally validated using pseudotyped viral particles. (virongy.com)
506 nm) reporter gene, packaged as viral particles which are pseudotyped with the SARS coronavirus spike protein (S-protein), which recognizes and binds to the ACE2 cell surface receptor to mediate coronavirus cell entry. (nucleusbiotech.com)
The infectiousness of ZIKV pseudotyped HIV luc particles were tested in Vero-B4 cells using firefly luciferase to monitor infection efficiency. (promegaconnections.com)
How would ZIKV pseudotyped virus particles fare when tested for viral entry using glioblastoma cell lines? (promegaconnections.com)
This indicated that the ZIKV pseudotyped viral particles were able to infect cultured glioblastoma cells. (promegaconnections.com)

Anti-CD19 CAR-NK cells were generated by transduction with baboon envelope pseudo-typed gamma-retroviral vectors. (bmj.com)

This was achieved by pseudotyping lentiviral vectors with a chimeric glycoprotein allowing for retrograde transport in combination with optimizing the promoter element used. (lu.se)

Compared to adenovirus and AAV, retrovirus-based vectors show relatively low transduction efficiency in-vivo , but their advantage lies in their ability to integrate stably and confer long-term transgene expression in dividing tissues. (biopharminternational.com)
In addition, IgG4 responses were not induced post-heterologous or homologous doses of the COVID-19 adenovirus-vectored vaccines. (news-medical.net)
Pseudotyped αvβ6 integrin-targeted adenovirus vectors for ovarian cancer therapies. (cardiff.ac.uk)

AAV-derived vectors (rAAV) are produced by double or triple plasmid transfection in HEK293T cells. (cnic.es)
When the ZIKV envelope protein vector was used in this three-plasmid system, Vero-B4 and U87 cells were infected at similar levels but the luminescence for 86HG39 cell was much lower but still above background. (promegaconnections.com)

Alphaviruses are of interest to gene therapy researchers, in particular the Ross River virus, Sindbis virus, Semliki Forest virus, and Venezuelan Equine Encephalitis virus have all been used to develop viral vectors for gene delivery. (genetherapynet.com)
However, the expression of Sindbis virus envelopes may lead to apoptosis, and their introduction into host cells upon infection by Sindbis virus envelope pseudotyped retroviruses may also lead to cell death. (genetherapynet.com)

The process of changing the tropism of the vectors is referred to as pseudotyping. (biopharminternational.com)

Several features of lentiviral vectors have made them advantageous for the expression of therapeutic proteins in vivo. (jci.org)
Some vectors, such as adenoviruses, have a fairly broad tropism, and the target range is often modified by engineering part of the envelope proteins. (biopharminternational.com)
Most of the ZIKV pseudovirus work used a two-vector HIV-1 system, one vector expressing the ZIKV envelope proteins and the other an HIV-1 vector that contained the reporter firefly luciferase. (promegaconnections.com)
Technique in which endogenous ENVELOPE PROTEINS have been replaced by use of a chimeric vector. (bvsalud.org)

Viral vector-mediated gene delivery can be carried out in two ways: ex vivo or in vivo. (themedicinemaker.com)
This feature constitutes an opportunity to personalize the vectors, and, consequently, to render gene delivery even more efficient. (themedicinemaker.com)

When pseudotyped lentiviral vectors encoding green fluorescent protein (GFP) were incubated with mouse spermatozoa, these sperm were highly successful in producing transgenics. (science20.com)
DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. (snmjournals.org)

By vaccinating hamsters with a recombinant parainfluenza virus vector, Buchholz et al. (cdc.gov)
In this study, we investigated the cellular and behavioral effect of overexpression of wild-type (WT), S87A, and S87E α-syn to block or to mimic S87 phosphorylation, respectively, in the substantia nigra of Wistar rats using recombinant adeno-associated vectors. (jneurosci.org)
Toward this goal, we engineered and stereotaxically injected recombinant adeno-associated vectors (rAAV2/6) overexpressing the WT, S87A or S87E α-syn into the rat SNc. (jneurosci.org)

In this study, we tested whether preventative and therapeutic vaccination could be achieved by direct injection of antigen-expressing lentiviral vector, obviating the need for ex vivo transduction of dendritic cells. (jci.org)
In vivo and ex vivo gene therapy, including characteristics of the different types of vectors (viral and non-viral) used for gene transfer as well as their advantages and disadvantages, administration routes and applications of gene therapy in the treatment of hereditary and non-hereditary human diseases. (uab.cat)

Lentiviruses are vectors derived from HIV-1 virus genome and are produced by co-transfection of two (2nd generation) or three (3rd generation) plasmids in HEK 293T cells. (cnic.es)

Depending on the application, the ideal vector either has an extremely broad tropism or extremely narrow if the objective is gene therapy of a very specific cell type (in this case this is a safety precaution)," Carstens elucidates. (biopharminternational.com)

Lentiviral vector-based dendritic cell vaccines induce protective T cell responses against viral infection and cancer in animal models. (jci.org)
Injection of a lentiviral vector encoding an MHC class I-restricted T cell epitope of lymphocytic choriomeningitis virus (LCMV) and CD40 ligand induced an antigen-specific cytolytic CD8 + T lymphocyte response that protected the mice from infection. (jci.org)
In an R&D setting, this becomes preferable if the same reagent is transfected (for example, CAS9 delivery vectors) since all the work is in the generation of the viral stock, not in the infection," Carstens says. (biopharminternational.com)
Determine Multiplicity of Infection (MOI) for each cell line and lentiviral vector combination you will use. (sigmaaldrich.com)
The neutralization values of the serum samples collected from hospitalized patients with COVID-19 or SARS-CoV-2 PCR-negative donors against the pseudotyped virus infection evaluated by the CRNT were compared with antibody titers determined from an enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay (IFA). (biomedcentral.com)
As a result, the inhibition of pseudotyped virus infection was specifically observed in both serum and whole blood and was also correlated with the results of the IFA. (biomedcentral.com)
Use of these types of vectors are useful in developing NEUTRALIZATION TESTS by enabling infection of almost universal set of host cells. (bvsalud.org)

Development of Lentiviral Vectors Pseudotyped With Influenza B Hemagglutinins: Application in Vaccine Immunogenicity, mAb Potency, and Sero-Surveillance Studies. (ox.ac.uk)
Further, sera from another cohort were analyzed to compare the immunogenicity of the heterologous and homologous regimens using the adenoviral vector-based ChAdOx1 vaccine and Comirnaty mRNA vaccine. (news-medical.net)
Taken together, our results indicate that LV vectors can serve to design pDC-based vaccines in humans, and they are also useful in vitro to evaluate the immunogenicity of the vector preparations, and the specificity and safety of given promoters used in gene therapy protocols. (biomedcentral.com)

The SARS-CoV-2 protein expression vectors are full-length, and codon optimized for mammalian cell expression. (virongy.com)

Alphaviruses are apt to be engineered to create replicon vectors which efficiently induce humoral and T-cell immune responses. (genetherapynet.com)

Application of replication-deficient vectors leads to short-term expression, which makes these vectors highly attractive for cancer gene therapy. (genetherapynet.com)

Vaccination by direct injection of lentiviral vector was more effective in sterile alpha motif and HD-domain containing protein 1-knockout (SAMHD1-knockout) mice, suggesting that lentiviral vectors containing Vpx, a lentiviral protein that increases the efficiency of dendritic cell transduction by inducing the degradation of SAMHD1, would be an effective strategy for the treatment of chronic disease in humans. (jci.org)

These properties have made lentiviral vectors advantageous for use in dendritic cell (DC) vaccines. (jci.org)

Alphavirus vectors carrying therapeutic or toxic genes used for intratumoral injections have demonstrated efficient tumor regression. (genetherapynet.com)

We next tested the efficacy of this vector in rhesus macaques with intrastriatal injections of Olig001 expressing GFP. (biomedcentral.com)

Didier Trono s'est beaucoup intéressé aux interactions entre les virus et leurs hôtes, ce qui l'a conduit à étudier la biologie de pathogènes comme le VIH et le virus de l''hépatite B, et à créer des outils de transfert génétique dérivés du VIH aujourd'hui utilisés avec succès en thérapie génique. (epfl.ch)
The injection of chronically infected mice with a lentiviral vector encoding LCMV MHC class I and II T cell epitopes and a soluble programmed cell death 1 microbody rapidly cleared the virus. (jci.org)
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable? (biopharminternational.com)
Carstens points out that the greatest limitation of viral vector systems is their tropism, that is, the type of cells that a virus will naturally infect. (biopharminternational.com)
By using the reporter gene firefly luciferase, researchers demonstrated that a pseudotyped virus could infect cultured glioblastoma cells. (promegaconnections.com)
To create a ZIKV pseudotyped virus, an HIV-1 vector expressing firefly luciferase (HIV luc) was cotransfected with four different pr ME expression vectors. (promegaconnections.com)

Alternatively, the DCs are transduced with a viral vector that expresses the antigen. (jci.org)

The stable integration of viral genes is mediated by the retroviral interiors of these vectors. (genetherapynet.com)
In a mouse model, introducing new genetic material via a viral vector into the sperm of mice leads to the presence and activity of those genes in the resulting embryos. (science20.com)

Drug developers choose from a vast array of potential vectors by considering several key aspects, including the disease being treated, the characteristics and location of cells that need to be targeted, the amount of genetic material needing to be delivered. (themedicinemaker.com)

Methods Plasma collected from 175 COVID-19 recovered patients with mild symptoms were screened using a safe and sensitive pseudotyped-lentiviral-vector-based neutralization assay. (medrxiv.org)

each of the vectors has its own advantages, limitations, and range of applications," Vicaire says. (biopharminternational.com)

Explain the characteristics of the different types of vectors used in gene transfer, their advantages and disadvantages, and their use for each disease or tissue. (uab.cat)

For all practical purposes, a viral vector is a genetic element wrapped in its own transfection reagent, which makes transfection titrations possible (e.g., dosage responses) and highly effective. (biopharminternational.com)

If the cells causing the disease can be collected from the patient, corrected in a dish, and then reinfused back into the patient, there is no need to give the vectors specific signals for which cells to infect. (themedicinemaker.com)

The most commonly used lentiviral vector producer cell line is HEK293. (themedicinemaker.com)

Each viral vector system is characterized by an inherent set of properties that affect its suitability for gene therapy or other specific applications, says Florence Vicaire, global gene therapy business development leader, Cytiva (formerly part of GE Healthcare). (biopharminternational.com)

Email: [email protected] to ask us about a custom expression vector order. (virongy.com)
We utilise monosynaptic tracing technology, using pseudotyped rabies vectors, in order to visualise the synaptic contacts formed between host neurons and graft human cells. (lu.se)

Injected lentiviral vector preferentially transduced splenic dendritic cells and resulted in long-term expression. (jci.org)
In both cases, cells may be treated either with a viral or non-viral vector carrying the corrected copy of the gene. (biopharminternational.com)
We found that efficient gene transfer into pDC can be achieved with VSVG-pseudotyped lentiviral vectors (LV) under the control of phoshoglycerate kinase (PGK) and elongation factor-1 (EF1α) promoters (28% to 90% of E-GFP + cells, respectively) in the absence of phenotypic and functional maturation. (biomedcentral.com)

Surprisingly, promoters (desmin or synthetic C5-12) described as muscle-specific and which drive gene expression in single strand AAV vectors in gene therapy protocols were very highly active in pDC using VSVG-LV. (biomedcentral.com)
The ZIKV pME-Z1 expression vector generated the highest luminescence and was selected for use in subsequent experiments. (promegaconnections.com)

To evaluate the potential of our vector to elicit MSA-like pathology in NHPs, we injected rhesus macaques intrastriatally with Olig001 expressing the α-syn transgene. (biomedcentral.com)

The potential opportunity of a vector depends on the type of gene therapy being developed, says Gaurav Chaudhary, CEO of Roots Analysis, a business research and consulting firm. (biopharminternational.com)

Agilent vectors, for example, are suitable and compatible with generation-four packaging systems (i.e., the latest version of packaging systems). (biopharminternational.com)
We offer third-generation replication-incompetent lentiviral vectors expressing the tagGFP2 (ex. (nucleusbiotech.com)

Results: We transduced on average 4000 neurons of the direct pathway in the striatum, with the viral vector allowing for microscopy and miRNA immunoprecipitation. (lu.se)

Carstens cautions, however, that biomanufacturers need to be aware that such modifications are properties of the packaging system, not the viral vector. (biopharminternational.com)

Anatomy20

Organisms45

Diseases2

Chemicals and Drugs36

Analytical, Diagnostic and Therapeutic Techniques and Equipment10

Phenomena and Processes26

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science4

Health Care6

Lentivirus4

Viruses9

Vesicular5

Adeno-assoc1

Adenoviral vectors1

Particles7

Retroviral Vectors1

Glycoprotein1

Adenovirus3

Plasmid2

Sindbis Virus2

Pseudotyping1

Proteins4

Gene delivery2

Green fluores2

Recombinant3

Vivo2

Lentiviruses1

Broad tropism1

Infection7

Immunogenicity3

Protein expression1

Induce1

Cancer gene therapy1

Efficiency1

Vaccines1

Therapeutic1

Injections1

Virus6

Antigen1

Genes2

Genetic material1

Neutralization1

Limitations1

Tissue1

Transfection1

Signals1

Cell1

Development1

Order2

Cells3

Expression2

Evaluate1

Potential1

Generation2

Results1

System1