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Plasmids8
  • Unlike plasmids, they can also be packaged in vitro into phage capsids, a step which requires cohesive ends, also known as cos sites also used in cloning with a lambda phage as a vector, however nearly all the lambda genes have been deleted with the exception of the cos sequence. (wikipedia.org)
  • Since there is a requirement for in vitro packaging whereby at least 38 kb of DNA is required between the cos sites, the vector without insert DNA will not be packaged (plasmids instability is increased if the novel inserted DNA contains many direct repeats or palindromic (inverted repeats) DNA. (wikipedia.org)
  • These extracts will recognize and package the recombinant molecules in vitro, generating either mature phage particles (lambda-based vectors) or recombinant plasmids contained in phage shells (cosmids). (wikipedia.org)
  • Isolation of clonable DNA (genomic libraries, cDNA synthesis) cloning vectors (plasmids, bacteriophages, cosmids) plasmid incompatibility and control of copy number. (up.ac.za)
  • There are a variety of cloning vectors available, many based on naturally occurring molecules such as bacterial plasmids or bacteria-infecting viruses. (biologydiscussion.com)
  • Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
  • Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
  • Prokaryotic organisms normally contain small, circular, extrachromosomal DNA molecules called plasmids that can serve as vectors. (pharmacy180.com)
Plasmid3
  • First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)
  • Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)
  • Recombinant clones were constructed in the plasmid vector pBluescript II KS(+) (Stratagene, La Jolla, Calif.). Chemicals, media, and growth conditions. (careersfromscience.org)
Fragments17
  • This invention introduces novel methods and compositions for the preparation of a genomic library, specifically a mouse genomic library, for producing knock-out targeting vectors by arraying into pools bacteria transformed with the yeast-Escherichia coli shuttle vector comprising mouse genomic DNA fragments. (cellmedicine.com)
  • Further disclosed are the procedures for preparing a genomic library for use in producing knockout targeting vectors, including details of genomic DNA fragments, bacterial selection markers, yeast replication, and the amplification of shuttle vectors. (cellmedicine.com)
  • Target DNA: the genomic DNA to be cloned has to be cut into the appropriate size range of restriction fragments. (wikipedia.org)
  • Resolving a critical metabolic bottleneck in C. cellulolyticum has been performed using an over-expression genomic library to identify gene fragments that impart a growth advantage on cellulosic substrates. (aiche.org)
  • This approach allowed for isolation and amplification of genomic fragments from environmental sources rich in biological and cellulolytic activity. (aiche.org)
  • These DNA fragments clone at high efficiency to commercial TOPO™ cloning vectors and are easily transposed into other vectors using the Gateway™ cassette system. (aiche.org)
  • Expressing the library in continuously sub-cultured C. cellulolyticum grown on different cellulosic carbon sources (including recycled paper) allowed for identification of unique gene fragments that resolved metabolic bottlenecking and improved cellulose degradation rates. (aiche.org)
  • Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. (harvard.edu)
  • A genomic library consisting of partially digested 10 to 20 kilobase pair fragments of Treponema pallidum deoxyribonucleic acid (DNA) was constructed using bacteriophage lambda EMBL-3 as the vector. (bmj.com)
  • A DNA library is a set of cloned fragments that collectively represent the genes of a particular organism. (biologydiscussion.com)
  • By digesting complex genomic DNA from an organism it is possible to reproducibly divide its genome into a large number of small fragments, each approximately the size of a single gene. (biologydiscussion.com)
  • This means that digesting genomic DNA with EcoRI, which recognizes the sequence 5′-GAATTC-3′, will produce fragments each of which is, on average, just over 4 kb. (biologydiscussion.com)
  • Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
  • A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)
  • Sequencing and genomic localization of a library of chromatin-DNA fragments bound by Pax7 has identified 34 candidate Pax7 target genes, with occupancy of a selection confirmed with independent chromatin enrichment tests (ChIP-PCR). (edu.au)
  • A DNA library is a collection of cloned restriction fragments of the DNA of an organism. (pharmacy180.com)
  • A genomic library is a collection of fragments of dsDNA obtained by digestion of the total DNA of the organism with a restriction endonuclease and subsequent ligation to an appropriate vector. (pharmacy180.com)
Phage3
  • Phage libraries are also stored and screened more easily than cosmid libraries. (wikipedia.org)
  • A recombinant phage library was constructed in the phage vector gt11 (Promega) using partially digested strain R expressing the 55-kDa product of PvpA (49). (careersfromscience.org)
  • Genomic library is normally made by PDF, 'DNA Libraries MCQ' App Download (Free) with α phage vectors, λ phage vectors, β phage vectors, and γ phage vectors choices for best online colleges with financial aid. (mcqslearn.com)
CDNA libraries6
Constructs3
  • The present invention is directed to methods for producing gene targeting constructs by homologous recombination using mouse genomic libraries arrayed in yeast shuttle vectors. (cellmedicine.com)
  • The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. (justia.com)
  • To assess the capacity of Pax7 to regulate transcription from these loci, we have cloned alternate transcripts of Pax7 (differing significantly in their DNA binding domain) into expression vectors and transfected cultured cells with these constructs, then analyzed target gene expression levels using RT-PCR. (edu.au)
Cosmids2
Cosmid libraries2
  • After the construction of recombinant lambda or cosmid libraries the total DNA is transferred into an appropriate E. coli host via a technique called in vitro packaging. (wikipedia.org)
  • The B16-melanoma DNA cosmid libraries were constructed with the shuttle vector pCV103. (go.jp)
Yeast2
  • Chromosomes, Artificial, Yeast" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
Organism3
  • To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
  • CRISPR/Cas9 screens, which have revolutionised human and model organism research, have not yet been implemented in malaria parasites due to the need for more complex CRISPR/Cas9 gene targeting vector libraries. (portlandpress.com)
  • Since the genome of an organism is fixed, chromosomal DNA may be isolated from almost any cell type in order to prepare genomic DNA. (biologydiscussion.com)
Bacterial2
  • Expression of recombinant proteins using bacterial, animal and plant vectors. (aadharinstitute.com)
  • Total DNA was isolated from the transfectants, and the B16 genomic DNA clones were rescued by the in vitro packaging with lysogenic bacterial extracts. (go.jp)
Expression7
  • A novel construct for inducible expression of a mature microRNA, shMIMIC Inducible Lentiviral microRNA pooled libraries are available to target every human and mouse microRNA within in the miRBase database ( www.mirbase.org ). (horizondiscovery.com)
  • Choose shMIMIC Lentiviral microRNA Pooled Libraries for constitutive expression of mature microRNAs. (horizondiscovery.com)
  • The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
  • Expression vectors are designed for expressing the protein encoded by the inserted gene. (biologyexams4u.com)
  • To also target low expressed essential genes, we included some variant steps that were expected to overcome the non-stringent regulation of the promoter carried by the expression vector used for the shotgun antisense libraries. (biomedcentral.com)
  • Because cDNA has no intervening sequences, it can be cloned into an expression vector for the synthesis of human proteins by bacteria or eukaryotes. (pharmacy180.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
Genes6
  • The gene encoding the Ov-phy-1 open reading frame contained 11 introns, similar in structure to the gene encoding human prolyl 4-hydroxylase isoform I. Genomic Southern blot, EST and genomic PCR studies demonstrated that the O. volvulus genome contained between three and eight genes closely related to Ov-phy-1. (embl.de)
  • Particular genes can be isolated from DNA libraries, much as books can be obtained from conventional libraries. (biologydiscussion.com)
  • Finally, we succeeded in cloning of the genomic deoxyribonucleic acid (DNA) which contains genes encoding melanoma antigen with species specific determinant (M562). (go.jp)
  • The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
  • Should have one or more marker genes for identification ad isolation of subpopulation of bacteria containing vector. (biologyexams4u.com)
  • For example, even if comprehensive insertion libraries are produced, it is inevitable that some genes, especially the shortest ones, could elude insertion and be spuriously annotated as essential, while transposon insertions that occur at gene ends and do not fully inactivate the function could lead to genes being incorrectly classified as non-essential. (biomedcentral.com)
Murine1
  • A 9.3-kb EcoRI fragment containing the first exon of the PKCe gene was isolated from a murine 129/Sv genomic DNA library. (ximbio.com)
Cloning7
Sequencing2
  • Library construction, pooling techniques, and high-throughput sequencing-compatible screening workflows have been experimentally validated to ensure reproducibility and accurate hit identification. (horizondiscovery.com)
  • Building of genomic DNA library, screening of CMCase-generating clones, and sequencing. (insulin-receptor.info)
Sequence2
  • A coding sequence (CDS) fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics. (besjournal.com)
  • Vectors must be capable of autonomous replication within the host cell, must contain at least one specific nucleotide sequence recognized by a restriction endonuclease, and must carry at least one gene that confers the ability to select for the vector such as an antibiotic resistance gene. (pharmacy180.com)
Molecule1
Restriction2
  • After genomic DNA has been isolated and purified, it is digested with restriction endonucleases. (biologydiscussion.com)
  • The App DNA Libraries MCQ e-Book PDF Download: dna technology applications, stem cells, gel electrophoresis and southern blotting, restriction enzymes test prep for pre employment screening tests. (mcqslearn.com)
Probes1
  • Probes can be used to identify which clone of a library or which band on a gel contains the target DNA. (pharmacy180.com)
19771
  • Commonly used vectors include "SuperCos 1" Hohn B, Murray K (August 1977). (wikipedia.org)
Lentiviral3
  • Pooled lentiviral libraries offer an efficient and cost-effective method for screening large numbers of microRNAs without automation. (horizondiscovery.com)
  • Learn about optimal library design and the pooled screening workflow on our Pooled Lentiviral Screening Libraries page. (horizondiscovery.com)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Populations1
  • The geographic distribution of VL is increasing due to human migration and travel, as well as the spread of vector populations to previously unaffected regions [ 4 ]. (biomedcentral.com)
Cells1
Clones1
  • The genomic library was screened for CMCase-producing clones CLEC4M by using an overlay of 0.7% (wt/vol) top agar containing 0.2% (wt/vol) carboxymethyl cellulose (CMC). (insulin-receptor.info)
Vivo1
  • XLOLR (Stratagene) was used for in vivo excision of the pBK-CMV phagemid vector from the ZAP Express vector using the ExAssist/XLOLR system (Stratagene). (insulin-receptor.info)
Genomes1
  • Scientists at the Centers for Disease Control and Prevention (CDC) are using information about human, vector, and pathogen genomes to tackle diverse public health problems, from newborn metabolic disorders to infectious disease outbreaks . (cdc.gov)
Generate1
  • A novel, template-independent approach involving degenerated oligonucleotide primed PCR was used to generate genomic libraries reliably and rapidly from the genomic DNA of multiple bacteria with different methylation characteristics. (aiche.org)
Fragment1
  • The choice of the particular type of gene library depends on a number of factors, the most important being the final application of any DNA fragment derived from the library. (biologydiscussion.com)
Molecular1
  • These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms. (harvard.edu)
Bacterium1
  • In order to advance study on the mechanism of cellulose degradation by this bacterium, we tried to isolate a gene encoding CMCase from the genomic DNA library of 5. (insulin-receptor.info)
Construction1
Protein1
  • If the ultimate aim understands the control of protein production for a particular gene or its architecture, then genomic libraries must be used. (biologydiscussion.com)
Articles1
  • A fourth collection of CDC-authored articles is identified from the "Genetics and Genomics" category in CDC Science Clips , a bibliographic digest produced by the CDC Library. (cdc.gov)
Species1
  • Leishmaniasis refers to a broad group of vector-borne parasitic diseases caused by species of the genus Leishmania . (biomedcentral.com)
Human1
  • shMIMIC pooled libraries are offered for all human and mouse miRBase matures, as well as a Human/Mouse Conserved collection. (horizondiscovery.com)
Generation1
  • Also unique to our new method is that error-prone PCR is supported in library generation, dramatically expanding the search space for improved biological activity. (aiche.org)
Infection1
  • These differences are reflected in the different infection frequencies seen in favour of lambda-replacement vectors. (wikipedia.org)
Biology1