Vascular tissue in vitro and in vivo hematopoietic and vascular. Medical search. Frequent questions

PD-L1 is expressed not only by all hematopoietic cells but also by many non-hematopoietic cell types such as endothelial cells and epithelial cells ( Sharpe and Pauken, 2018 ). (frontiersin.org)
We determined the presence of EndoMT in the pulmonary vasculature in vivo and the functional effects on pulmonary artery endothelial cells (PAECs) undergoing EndoMT in vitro. (surrey.ac.uk)
During this year, we have demonstrated that hematopoietic stem cells are originated from the cells that line the inside of blood vessels, named endothelial cells. (ca.gov)
Budding of hematopoietic stem cells from endothelial cells occurs during a specific and restricted time window during development and progress has been made to elucidate the regulatory genetic networks involved in this process. (ca.gov)
This information will be used to recapitulate similar conditions in vitro and induce the growth of hematopoietic stem cells outside the body from adult endothelial cells. (ca.gov)
The first step was to test whether human endothelial cells could offer a supportive niche for the growth of hematopoietic cells. (ca.gov)
During embryonic development mesodermal differentiate into endothelial cells, hemangioblast and hematopoietic progenitors give rise to blood vessel, the vasculogenesis. (samploon.com)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)

Development of new blood vessels is necessary for the effective tissue regeneration. (hindawi.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Hence the discovery is reported simply by us of potential adult stem cells which may be involved with tissue regeneration. (cancer-pictures.org)
Tissue engineering is a multidisciplinary field that applies the principles of engineering and life-sciences for regeneration of damaged tissues. (wjgnet.com)
The increasing recognition of the contribution of the immune system to activate and prime regeneration implies that tissue engineering strategies and biomaterials design should target regulation of early immunological processes. (bvsalud.org)
These results bear relevance towards the manufacturing of potent off-the-shelf osteoinductive materials and outline the broader paradigm of engineering immunoinstructive implants to enhance tissue regeneration. (bvsalud.org)
ASCs are present in a number of posnatal tissues and are responsible for normal tissue renewal as well as for regeneration and healing after injuries. (bvsalud.org)
Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
Tissue regeneration approach using stem cells in different clinical settings is a medical sensation now-a-days. (samploon.com)
Human endometrium is highly regenerative tissue since its complete regeneration of the uterus was achieved after an almost total resection of endometrium and the renovated endometrium could support the gestation. (samploon.com)
EMSCs also have the high capacity proliferation, multidirectional differentiation that is indicative for the potentiality for tissue regeneration. (samploon.com)

Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
This protocol generates cells that resemble human foetal liver hematopoietic stem/progenitor cells (HSPC) by immunophenotype (CD34+CD38loCD90+CD45+GPI80+/-) and molecular profile. (researchsquare.com)
Preliminary data from our lab using mouse xenograft models show that breast cancer cells (BCCs) metastasize to and lay dormant in unique perivascular hematopoietic progenitor and stem cell (HSPC) niches. (grantome.com)

In the second year of this proposal, we have made significant progress and identified critical factors that are responsible for the generation of hematopoietic stem cells from the endothelium (inner layer of blood vessels). (ca.gov)
Specifically, we have tested whether forced expression of these genes could induce the generation of hematopoietic stem cells. (ca.gov)

Calcifying vascular cells are derived from local smooth muscle cells and circulating hematopoietic stem cells (especially in intimal calcification). (clinsurgeryjournal.com)

Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Although previous protocols have generated clonogenic progenitors and various differentiated hematopoietic cells, there is no confirmation of their definitive identity or functional HSC properties. (researchsquare.com)
Many protocols have been described that achieve the generation of clonogenic progenitors and various differentiated hematopoietic cells. (researchsquare.com)
It has been proposed that hematopoietic differentiation from pluripotent stem cells is biased toward the generation of the yolk sac like differentiation-primed progenitors. (researchsquare.com)

CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Here, we describe a two-step differentiation protocol for H1 hESC that combines 2 weeks of embryoid body differentiation in mesoderm inducing conditions followed by OP9M2 stroma co-culture in HSC supportive conditions, which enables the generation of hematopoietic cells that resemble foetal liver HSPCs by immunophenotype (CD34+CD38loCD90+CD45+GPI80+) 2 and molecular profile. (researchsquare.com)

The adult stem cell can be labeled in vivo and tracked, it can be isolated and then transplanted back into the organism, and it can be isolated in vivo and manipulated with growth hormones. (wikipedia.org)
However, it is known that in vitro cell culture conditions can alter the behavior of cells, proving that a particular subpopulation of cells possesses stem cell properties in vivo is challenging, and so considerable debate exists as to whether some proposed stem cell populations in the adult are indeed stem cells. (wikipedia.org)
VSELs have already been described as uncommon little pluripotent stem cells within murine and individual BM [7 8 Kaempferol-3-O-glucorhamnoside They're lineage- and Compact disc45-harmful express stem-cell markers and present rise to cells matching to all or any three germ levels in vitro [9]. (cancer-pictures.org)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
Stem cell behaviour can also be controlled in vitro by manipulating the elasticity of both porous and non-porous materials, yet translation to therapeutic processes in vivo remains elusive. (nature.com)
Here, by developing injectable, void-forming hydrogels that decouple pore formation from elasticity, we show that mesenchymal stem cell (MSC) osteogenesis in vitro , and cell deployment in vitro and in vivo , can be controlled by modifying, respectively, the hydrogel's elastic modulus or its chemistry. (nature.com)
Stem cell therapy is an exciting and upcoming branch of tissue engineering with application in the field of orthopaedics. (openorthopaedicsjournal.com)
Nevertheless, stem cell based tissue engineering in orthopaedics shows a promising future. (openorthopaedicsjournal.com)
Epidermal stem cells - localization of stem cells, epidermal stem cell markers, epidermal stem cells in vitro and in vivo, application of epidermal stem cells in medicine. (edu.pl)
Guidelines for preventing opportunistic infections among hematopoietic stem cell transplant recipients. (cdc.gov)

1. Wound healing a) Inflammatory phase of wound healing - vascular response, cellular response, mediators of inflammation, factors modulating the inflammatory reaction (acetylsalicylic acid, steroids). (edu.pl)

The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
The generation of definitive, transplantable haematopoietic stem cells (HSC) from pluripotent stem cells (PSC) is an unmet challenge. (researchsquare.com)
The attempts to generate definitive, transplantable hematopoietic stem cells (HSC) from human pluripotent stem cells have not been successful. (researchsquare.com)

Induced EndoMT cells exhibited up-regulation of mesenchymal markers, including collagen type I and α-smooth muscle actin, and a reduction in endothelial cell and junctional proteins, including von Willebrand factor, CD31, occludin, and vascular endothelial-cadherin. (surrey.ac.uk)
CD44 is expressed in many normal human hematopoietic, epithelial,and mesenchymal cells. (roche.com)
The most commonly used type of stem cells, mesenchymal stem cells (MSCs), can be easily isolated from bone marrow or synovium and cultured in vitro . (openorthopaedicsjournal.com)
Human endometrial tissue composed of epithelial stem cells (0.22%), mesenchymal stem cells (1.25%) and side population stem cells. (samploon.com)
c) Miogenic potential of stem cells - hematopoietic stem cells, mesenchymal stem cells, stem cells derived from blood vessels, embryonic stem cells. (edu.pl)

Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
The only stimulant that enhanced PAC paracrine angiogenic activity was atorvastatin, which also had ability to stabilize endothelial tubes in vitro . (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
Microtubule depolymerization rapidly collapses capillary tube networks in vitro and angiogenic vessels in vivo through the small GTPase Rho. (xenbase.org)

Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)

Normally, the endothelium forms an integral cellular barrier to regulate vascular homeostasis. (surrey.ac.uk)
We have also demonstrated that hemogenic endothelium is derived from one specific embryonic tissue (lateral plate mesoderm). (ca.gov)
Definitive HSCs are generated in a narrow time window of development in the major arteries in the embryo and extraembryonic tissues from a specialized type of haemogenic endothelium. (researchsquare.com)

HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
Aging facilitates the expansion of hematopoietic stem cells (HSCs) carrying clonal hematopoiesis-related somatic mutations and the development of myeloid malignancies, such as myeloproliferative neoplasms (MPNs). (bvsalud.org)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)

and today is the most widely used in vitro assay for HSPCs. (stemcell.com)
While the embryoid body differentiation generates immature haematovascular precursors, co-culture on mouse bone marrow OP9M2 stroma 3 enables subsequent maturation step of the CD34+ haemato-vascular cells to immunophenotypic human HSPCs. (researchsquare.com)

White adipose tissue (WAT) has key metabolic and endocrine functions and plays a role in regulating energy homeostasis and insulin sensitivity. (springer.com)
Conversely, brown adipose tissue (BAT) and browning of WAT represent potential therapeutic approaches, since dysfunctional white adipocyte-induced lipid overspill can be halted by BAT/browning-mediated oxidative anti-lipotoxic effects. (springer.com)
Here, we summarise the mechanisms contributing to adipose tissue (AT) plasticity and function including characteristics and cellular complexity of the various adipose depots and we discuss recent insights into AT origins, identification of adipose precursors, pathophysiological regulation of adipogenesis and its relation to WAT/BAT expandability in obesity and its associated comorbidities. (springer.com)
White adipose tissue (WAT) plays a key homeostatic role, not only by ensuring efficient energy storage but also by its quick mobilisation (lipids) to ensure peripheral demands. (springer.com)
The 'adipose tissue expandability model' identifies the limited capacity and dysfunctionality of WAT, preventing its expansion and accommodation of surplus of energy, as key determinants for the onset and progression of obesity-associated metabolopathologies as a result of ectopic deposition of toxic lipid species in metabolic organs (i.e. muscle or liver [also known as lipotoxic insult]) [ 7 ]. (springer.com)
There is also evidence that the deleterious effects mediated by dysfunctional white adipocyte-induced lipid overspill can be halted by the pro-oxidative anti-lipotoxic effects mediated by brown adipose tissue (BAT) activation. (springer.com)
Fig. 1b, c ) or the pancreas and the white adipose tissue (WAT) that are infiltrated with MAC1-possitve macrophages expressing YFP (Fig. 1d ). (nature.com)
indicated that ATMs colocalized with T cells in lymphoid clusters within adipose tissue and may act as APCs, which express high levels of MHCII and also costimulatory molecules and process and present antigens to induce CD4+ T-cell proliferation and activation in adipose tissue of obese mice (29, 68, 105). (exposed-skin-care.net)
However, its role in aging-related adipose tissue CD4+ T cell activation remains to be investigated. (exposed-skin-care.net)
CD8+ T Cell Activation Compared to CD4+ T cells, CD8+ T cells show a greater increase in adipose tissue in obesity and in aging (31, 43, 106). (exposed-skin-care.net)
Similar to CD4+ T cells, CD8+ T cells exhibit effector memory or effector phenotypes expressing elevated levels of IFN- in obese adipose tissue (31, 44). (exposed-skin-care.net)
The mechanism for CD8+ T cell activation in adipose tissue is not fully understood. (exposed-skin-care.net)
showed that adipose tissue from obese mice induced proliferation of splenic CD8+ T cells, indicating a CD8+ T cell-activating environment in obese adipose tissue (31). (exposed-skin-care.net)
Indeed, CD8+ T cells from mouse adipose tissue respond to cytokines and become activated and proliferate under stimulation of IL-12 and IL-18, which are mainly produced by APCs and are elevated in obese adipose tissue (44). (exposed-skin-care.net)
The stromal fraction that is harvested from adipose tissue is a heterogeneous mixture of regenerative cells (see below). (vetstem.com)

While ER Clark first reported the observation of vascular generation in tadpoles in 1909, and Stockard described blood-vessel development and coined the term angioblast in fish embryos in 1915, it was not until 1920 that F Sabin described the formation of blood cells through the angioblast 'liquefying' within the aorta [ 1 - 3 ]. (biomedcentral.com)

Upon implantation, these IL1ß-induced materials enhanced processes typical of the inflammatory phase (e.g., vascular invasion, osteoclast recruitment and differentiation), leading to 'regenerative' events (e.g. (bvsalud.org)
The use of ASCs in regenerative medicine and tissue engineering research has important advantages in comparison with ESCs, since there are no ethical complications and the process of differentiation of these cells is better controlled. (bvsalud.org)
Cartilage heals very poorly, owing to the lack of intrinsic regenerative capacity of the chondrocytes and its poor vascular supply. (openorthopaedicsjournal.com)

Unlike embryonic stem cells, the use of human adult stem cells in research and therapy is not considered to be controversial, as they are derived from adult tissue samples rather than human embryos designated for scientific research. (wikipedia.org)
In addition, we developed a series of novel tools to test the biological relevance of the genes identified in vivo (using mouse embryos). (ca.gov)
Q�Z0 ) Flk1 -LacZ beta-galactosidase staining of littermate E8.5 (6 somite stage) Rasip1+/ and Rasip1 / embryos and tissues, in whole mount or section. (xenbase.org)

c) Phase of maturation and remodelling-changes in the structure of connective tissue, collagen resorption, factors affecting the remodelling (age, wound localization, wound type, duration of the inflammatory phase). (edu.pl)

Adult stem cells are undifferentiated cells, found throughout the body after development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. (wikipedia.org)
Newer techniques using tissue engineering to regenerate musculoskeletal tissue by using biomimetic materials are now being studied. (openorthopaedicsjournal.com)
This method aims to regenerate rather than repair skeletal tissue defects. (openorthopaedicsjournal.com)
is a broad definition for innovative medical therapies that will enable the body to repair, replace, restore, and regenerate damaged or diseased tissues. (vetstem.com)

10] reported they differentiate vivo into lung epithelial cells in. (cancer-pictures.org)

These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized. (wikipedia.org)
Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
Pulmonary arterial hypertension (PAH) is a progressive disease characterized by lung endothelial cell dysfunction and vascular remodeling. (surrey.ac.uk)
hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
Functionally, CD44 is involved in lymphocyte recirculation, adhesion, activation, lymph node homing, and to a larger extent in other tissue types, cell- cell and cell-matrix interactions. (roche.com)
4. Southgate J., Trejdosiewicz L. et al.Patterns of splice variant CD44 expression by normal human urothelium in situ and in vitro and by bladder-carcinoma cell lines. (roche.com)
To test these hypotheses, we will use single cell resolution, video rate in vivo microscopy in mouse xenograft models to track BCC migration through BM in real time. (grantome.com)
Figure 3: Controlling cell deployment kinetics from void-forming hydrogels in vitro and in vivo . (nature.com)
However, the main element substances that mediate T cell infiltration into adipose tissues in maturing remain to become discovered. (exposed-skin-care.net)
Activation of Typical T Cells in Adipose Tissues Compact disc4+ Purpureaside C T Cell Activation TCRs recognize the current presence of a particular antigen by binding to brief peptide sequences in the antigen that's shown on APCs. (exposed-skin-care.net)
Initial studies indicate an immense potential for cell based strategies to enhance current orthopaedic approaches in skeletal tissue reconstruction. (openorthopaedicsjournal.com)
The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)

This, in turn, establishes chronic inflammation and tissue pathology in mice with important ramifications for DNA repair-deficient, progeroid syndromes and aging. (nature.com)
Using mice with an engineered ERCC1-XPF defect in tissue-infiltrating macrophages, we provide evidence for a fundamental mechanism by which irreparable DNA damage triggers an exosome-based, metabolic reprogramming that leads to chronic inflammation and tissue pathology in NER progeroid syndromes and likely also during aging. (nature.com)

had higher sphere-forming efficiency and proliferative potential than VSELs and they were present to differentiate into neuronal cells in vitro. (cancer-pictures.org)

VEGF is also involved in regulation of vascular permeability. (samploon.com)

In contrast, PD-L2 expression is more restricted and can be induced on hematopoietic cells such as DCs, B cells, and monocytes/macrophages. (frontiersin.org)
Here, we show that persistent DNA damage accumulation in tissue-infiltrating macrophages carrying an ERCC1-XPF DNA repair defect ( Er1 F/− ) triggers Golgi dispersal, dilation of endoplasmic reticulum, autophagy and exosome biogenesis leading to the secretion of extracellular vesicles (EVs) in vivo and ex vivo. (nature.com)

Under normal conditions, tissue stem cells divide slowly and infrequently. (wikipedia.org)
In hematopoietic stem cells, the MAPK/ERK pathway and PI3K/AKT/mTOR pathway regulate this transition. (wikipedia.org)
It can be induced by modifying the growth medium when stem cells are cultured in vitro or by transplanting them to an organ of the body different from the one they were originally isolated from. (wikipedia.org)
The intravascular HAR-NDS might serve as a route that delivers these stem cells with their target tissues. (cancer-pictures.org)
Introduction Several adult non-hematopoietic Kaempferol-3-O-glucorhamnoside stem cells have already been identified within the bone tissue marrow (BM) up to now. (cancer-pictures.org)
The objective of this proposal was to identify factors that allow blood vessels to generate hematopoietic stem cells early in the embryonic stage. (ca.gov)
We predicted that precise information related to the operational factors in the embryo would allow us to reproduce this process in a petri dish and generate hematopoietic stem cells when needed (situations associated with blood transplantation or cancer). (ca.gov)
For this, we first investigated the genes that responsible for this hemogenic activity during development using mouse models and tissue culture cells. (ca.gov)
Vascular cells can undergo chondrogenic or osteogenic differentiation, causing mineralization of membranous bone and formation of endochondral bone. (clinsurgeryjournal.com)
These cells have the ability to generate progeny associated with definitive haematopoiesis (adult globin expressing erythroid cells and T-lymphocytes) but have limited expansion ability in vitro and cannot engraft in vivo. (researchsquare.com)
WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
All tissues and cells were derived from mice. (aai.org)
In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
In explant method, the dental tissue is placed on a plastic surface and the cells migrate out from the tissue fragment adhering to culture flasks or dishes ( Figure 1 ). (bvsalud.org)
In obese humans, adipocyte-secreted CCL20 may contribute to the deposition of Compact disc4+ helper and Compact disc8+ cytotoxic T lymphocytes within adipose tissues, possibly via connections with CCR6 which was upregulated on T cells in obese adipose tissues (100). (exposed-skin-care.net)
further defined that mostly huge adipocytes from obese adipose tissues exhibited an increased expression degree of MHCII substances and acted as APCs to activate Compact disc4+ T cells to secrete IFN- (103). (exposed-skin-care.net)
Amidst this, tissue engineering using stem cells has emerged as a hot topic of research and is a promising alternative to the traditional techniques of bone grafting. (openorthopaedicsjournal.com)
CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. (thermofisher.com)
In vitro cultures of isolated skin cells - 2D and 3D cultures. (edu.pl)

Vascular tubes are absent in all Rasip1 / vessels examined. (xenbase.org)

The selected candidate will work closely with Dr. Andrew Putnam and other members of his group on NIH-funded projects focused on creating functional vasculature in engineered tissue constructs. (fellowshipbard.com)

MSCs can be isolated from different locations, such as bone marrow, umbilical cord, placenta, adipose and dental tissues. (bvsalud.org)

It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
They have a wide cellular and tissue distribution, and play a major role in the mediation of biological responses. (medscape.com)

Recent studies have suggested that micro calcification in the fibrous cap may increase local tissue stress (depending on the proximity of one micro calcific locus to another, and the orientation of the micro calcification in reference to blood flow), resulting in plaque instability [1]. (clinsurgeryjournal.com)

3 Changes in the regulation of the different variant expression patterns in a variety of tissue types has been associated with differing prognosis. (roche.com)

This property forms the basis of in vivo assays of HSC function. (stemcell.com)

This research considered the remodelling aspects of these conditions using both in vitro and pre-clinical models. (surrey.ac.uk)

Banks of bone tissue: biological aspects, immunogenicity of transplants. (edu.pl)

and 3) Understand how cross talk with the peri-sinusoidal BM vascular niche mediates BCC dormancy and sensitivity to chemo- and hormonal therapies. (grantome.com)

DAMPs, damage-associated tissues and the molecular target structures and pathways. (cdc.gov)

Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)

b) Proliferation phase of wound healing - generation of epithelium, deposition of collagen, generation of scar tissue. (edu.pl)

The in vivo and in vitro data generated by this study indicates that a multi-TKI, targeting the MST1R/MET/TAM signaling pathways, may provide a more effective therapeutic strategy for the treatment of MPM as opposed to targeting MST1R alone. (frontiersin.org)
A number of traditional therapeutic approaches to repair skeletal tissue have been developed and hypothesised, but they have all presented with a variety of shortcomings. (openorthopaedicsjournal.com)

Engineering living bone tissue of defined shape on-demand has remained a challenge. (bvsalud.org)

Primitive and definitive hematopoietic development. (biomedcentral.com)

In vivo experiment performed without occurrence of immune rejection. (samploon.com)

Hutmacher, D. W. Scaffolds in tissue engineering bone and cartilage. (nature.com)

Prior to joining Surrey, her research centred around pulmonary hypertension and connective tissue diseases. (surrey.ac.uk)

However, the mechanisms underlying vascular "tubulogenesis" are only beginning to be unraveled. (xenbase.org)
Many of the ed either from external sources or excitation events are much closer in vitro and in vivo studies investi- through internal exposure as a re- together for low-energy charged par- gating the mechanisms underlying sult of ingestion, inhalation, dermal ticles, which are considered to be cancer risk from exposure to ionizing absorption, or injection of radionu- high-LET radiation. (who.int)

The evidence from growth factor enhanced tissue engineering studies for tissue healing looks very positive. (openorthopaedicsjournal.com)

Anatomy46

Organisms24

Diseases7

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes49

Disciplines and Occupations1

Technology, Industry, Agriculture9

Information Science4

Endothelial cells8

Regeneration11

Progenitor7

Generation of hematopoietic stem2

Circulating hematopoietic stem1

Progenitors7

CD344

Stem cell13

Mediators of Inflammation1

Pluripotent stem3

Mesenchymal5

Angiogenic6

Differentiation in vitro1

Endothelium3

HSCs4

HSPCs2

Adipose tissue15

Blood vessel1

Regenerative3

Embryos3

Maturation1

Regenerate4

Epithelial cells1

Cell17

Inflammation2

Differentiate1

Permeability1

Macrophages2

Cells19

Vessels1

Vasculature1

MSCs1

Cellular2

Fibrous1

Regulation1

Assays1

Clinical1

Immunogenicity1

Niche1

Molecular1

Distinct1

Proliferation1

Therapeutic2

Bone tissue1

Definitive1

Immune1

Cartilage1

Connective1

Mechanisms2

Growth1