Note to the Reader Please cite this work as: Williams RW, Gu J, Qi S, Lu L (2001) The genetic structure of recombinant inbred mice: High-resolution consensus maps for complex trait analysis. (nervenet.org)
Donor3
Clarence Little transplanted tumors across different strains and found rejection of transplanted tumors according to strains of host versus donor. (wikipedia.org)
A male individual from the donor strain is first crossed to a female from the host strain. (comprehensivephysiology.com)
The genome of the final consomic strain is the same as that of the host strain, with the exception of the MSY from the donor strain. (comprehensivephysiology.com)
Genomes2
The highly repetitive sequence content of these regions poses a major barrier to their molecular analysis, and the PAR and centromeres remain unassembled or incompletely assembled on many of highest quality reference genomes, including mouse. (jax.org)
Genomes of individuals strains typically incorporate 45-55 recombinations between parental chromosomes. (nervenet.org)
Recombination2
My research aims to understand the causes and consequences of variation in the mechanisms that govern DNA inheritance: chromosome segregation, recombination, and de novo mutation. (jax.org)
The final consensus maps are based on 1578 microsatellite loci and these maps have a cumulative length of approximately 1400 cM after adjustment for recombination frequencies of RI strains. (nervenet.org)
Rats7
However, relatively little information is available on humane aspects of anesthesia and euthanasia in neonatal mice and rats. (bvsalud.org)
The study demonstrates that CO may be a potential alternative for preweanling mice and rats at PND6 or older but is not appropriate for neonates at PND5 or younger. (bvsalud.org)
The current study was designed based on existing literature regarding minimal exposure times for preweanling rats and mice euthanized with carbon dioxide. (bvsalud.org)
The study evaluated the minimal time that adult rats and mice must remain in 100% carbon dioxide for death to be irreversible on removal. (bvsalud.org)
Adult rats (14 stocks and strains) and mice (more than 40 stocks and strains) were euthanized using a 50% volume per minute displacement rate of carbon dioxide for 2 min. (bvsalud.org)
These rats are then inbred to produce a stable inbred consomic strain with the substitution of one entire chromosome from the BN strain into the background of the SS strain. (comprehensivephysiology.com)
Parental strain1
In this study we have increased the density of microsatellite markers 2- to 5-fold in each of several major RI sets that share C57BL/6 as a parental strain (AXB, BXA, BXD, BXH, and CXB). (nervenet.org)
Chromosome3
Despite their fundamental significance for chromosome transmission and genome stability, little is known about the levels and patterns of genetic diversity across centromeres and the PAR, or the biological impacts of this variation. (jax.org)
Example of reduced blood pressure salt sensitivity resulting from substitution of chromosome 13 from the BN into the SS/Mcwi strain (SS‐13 BN consomic rat). (comprehensivephysiology.com)
Conversely, substitution of chromosome 18 from SS to the BN strain (BN‐18 SS ) resulted in a relatively small increase in MAP in response to the high‐salt diet. (comprehensivephysiology.com)
Laboratory4
The classical laboratory inbred mouse strains derive from a small, ancestral population of wild mice that were selectively bred for traits of interest by mouse fanciers in the late 19th and early 20th centuries. (jax.org)
As a consequence of their unique historical origins, the genetic diversity captured in laboratory mice represents an extremely limited sample of the diversity found in wild mouse populations. (jax.org)
Our prior work has demonstrated that wild mouse populations harbor numerous predicted functional and disease-associated alleles, the majority of which are not present in classical inbred mouse strains and have therefore never been experimentally tested in the laboratory. (jax.org)
When the use of laboratory animal is obligatory, the most appropriate specie, breed, and strain with the closest homology to humans must be chosen. (ommegaonline.org)
Genetic variation1
In parallel, we are pursuing experimental tests of the functional consequences of genetic variation across these loci. (jax.org)
Diversity2
The simplified and known genetic background of captive mouse populations such as the Collaborative Cross, BXD recombinant inbred strain panel, the Diversity Outbred population, and collections of diverse inbred strains render them uniquely powerful resources for studying the mechanisms of short-term genome evolution and genomic inheritance. (jax.org)
If distinct conformers and glycotypes of PrPd are titration that used monoclonal antibody (MAb) 6H4 after responsible for diversity of prion strains, then they would limited proteinase K digestion. (cdc.gov)
Elucidate1
We therefore performed the first comprehensive transcriptome-proteome comparison in a RTT mouse model to elucidate RTT pathophysiology, identify potential therapeutic targets, and further our understanding of MeCP2 function. (biomedcentral.com)
Females1
Males from the latter are backcrossed again to females from the host strain, the procedure being repeated ( n ) times. (comprehensivephysiology.com)
Frogs1
Microsatellite variation and population genetic structure of Anatolian mountain frogs. (krakow.pl)
Model5
My research leverages the power of the house mouse model system to address open questions in evolutionary genomics. (jax.org)
My work relied on wild-derived inbred mice as a model system, and I gained relevant practical experience with wild mouse husbandry, colony maintenance, and mouse reproductive phenotyping. (jax.org)
Molecular Model of missible to at least 7 species ( 1 ), resulting in propagation of Prion Transmission PrPd that retains the characteristic molecular signature of the original BSE prion strain ( 5 - 7 ). (cdc.gov)
To address this, we examined the role of natural variation in defining the susceptibility of genetically diverse individuals to protein aggregation and toxicity, using the Caenorhabditis elegans polyglutamine model. (biomedcentral.com)
Thus, our C. elegans model can serve as a powerful tool to dissect the contribution of natural variation to individual susceptibility to proteotoxicity. (biomedcentral.com)
Panel2
Consomic rat panel in which each of the 20 autosomes and the X and Y chromosomes from the BN strain were transferred onto the SS genetic background. (comprehensivephysiology.com)
To further dissect these relationships, we established a panel of 21 recombinant inbred lines that showed a broad range of aggregation phenotypes, independent of differences in expression levels. (biomedcentral.com)
Yet age of onset can vary by several decades in people carrying the same length polyglutamine expansion, and a large proportion of this residual variation is genetic in nature and may be due to polymorphisms in other genes [ 12 - 15 ]. (biomedcentral.com)
Host2
Resulting F1 males are then backcrossed to a female from the host strain to generate the N2 generation. (comprehensivephysiology.com)
Although mission barriers for particular TSE agents between species, their precise biochemical composition is a matter of debate, we used PMCA reactions to amplify PrPd associated with they are known to occur in a series of strains, each with vCJD ( 10 ), bovine BSE ( 11 ), ovine scrapie ( 12 ), and exper- a characteristic disease phenotype and host range ( 1 ). (cdc.gov)
Population2
With the broad goal of elevating the profile of wild mice in biomedical research, my group is pursuing population genomic investigations in wild mice to understand the natural evolutionary forces that shape variation in wild mouse populations. (jax.org)
CONCLUSIONS: We identified substantial variation in initial anakinra use in a real-world population of children with MIS-C, but no average short-term improvement in cardiovascular outcomes associated with early addition of anakinra to IVIG and/or glucocorticoids compared to IVIG and glucocorticoids alone. (cdc.gov)
Important2
Recombinant inbred (RI) strains of mice are an important resource used to map and analyze complex traits. (nervenet.org)
Resistance to protein aggregation and the ability to restrict its associated cellular dysfunction are independently controlled by the natural variation in genetic background, revealing important new considerations in the search for targets for therapeutic intervention in conformational diseases. (biomedcentral.com)
Birds1
Marsupial MHC genotypic variation lies between eutherian mammals and birds, taken as the minimal MHC encoding, but is closer in organization to that of nonmammals. (wikipedia.org)
Found2
A imental ovine BSE ( 13 ) and substrates prepared from hu- central event in neuropathogenesis of TSEs is conversion manized transgenic mouse brain tissue expressing each of of the normal cellular form of the prion protein (PrPC) to the 3 main PRNP polymorphic variants found in Caucasian the pathognomonic disease-associated isoform (PrPd) ( 2 ). (cdc.gov)
Insulin produces the dephosphorylation of only a small subset of proteins at discrete locations, whereas PP1 is ubiquitously expressed and is found in virtually all cellular compartments, suggesting that mechanisms exist for the targeted regulation of PP1 in insulin-responsive cells that selectively permit activation of the enzyme only at these sites. (jci.org)
Responsible1
Recently, RI strains have also been used to map QTLs that that are responsible for biometric variation in the architecture of the mouse CNS (Belknap et al. (nervenet.org)
Wide2
Recombinant inbred (RI) strains have been used to map a wide range of Mendelian loci and quantitative traits (Taylor, 1989). (nervenet.org)
Introgression of polyQ40 into three wild genetic backgrounds uncovered wide variation in onset of aggregation and corresponding toxicity, as well as alteration in the cell-specific susceptibility to aggregation. (biomedcentral.com)
Human1
The database currently holds approximately 2000 complexes with the majority from Saccharomyces cerevisiae, human and mouse. (stanford.edu)
Study2
This study was designed to assess the effectiveness of carbon monoxide (CO) as an alternative euthanasia agent for mouse and rat pups on postnatal days (PND) 0 to 12. (bvsalud.org)
Genetic differentiation among autochthonous and cosmopolitan goat breeds based on information from microsatellite markers - a preliminary study. (krakow.pl)
Rate1
These mice have reduced glycogen stores in adipose tissue, liver, heart, and skeletal muscle, corresponding with decreased glycogen synthase activity and glycogen synthesis rate. (jci.org)
Wild1
We are also actively involved in the development of new strain and genomic resources derived from wild-caught animals. (jax.org)
Older1
Insulin resistance in older PTG heterozygous mice correlates with a significant increase in muscle triglyceride content, with a corresponding attenuation of insulin receptor signaling. (jci.org)
Normal1
Although young PTG heterozygous mice initially demonstrate normal glucose tolerance, progressive glucose intolerance, hyperinsulinemia, and insulin resistance develop with aging. (jci.org)