• This thesis explores both sides of the dichotomy in an effort to reveal the mechanics of two biological systems - M13 filamentous bacteriophage and kinesin motor protein - and the properties of their underlying parts. (mit.edu)
  • The new system, called phage-assisted continuous evolution, or PACE, is based on the continuous culture and selection of the M13 filamentous bacteriophage commonly used in phage display. (genengnews.com)
  • M13 is a filamentous virus that has elicited the interest of the engineering community for its use in phage display of combinatorial peptide libraries and more recently as a 1D template for organizing and growing inorganic materials. (mit.edu)
  • IgG variants with improved FcRn-binding were then isolated from these Fc-libraries using a pH-dependent phage display selection process. (biomedigs.org)
  • For his thesis work he established a labeling method for M13 bacteriophage to generate higher-order nano-material scaffolds and led a project to employ high-throughput sequencing for improved peptide phage display. (wisc.edu)
  • The workhorse of phage display is the M13 bacteriophage virus. (genomicglossaries.com)
  • Project entitled "A human cardiac troponin T electrochemical immunosensor based on M13 bacteriophages obtained via phage display" has been realised in our Group by Sebastian under supervision of Katarzyna Szot-Karpińska . (surfacenanogroup.com)
  • Clone of M13 bacteriophage displaying troponin T-specific peptide (selected by phage display) was immoblized on modified gold electrode. (surfacenanogroup.com)
  • The Harvard team demonstrated the utility of PACE in three separate experiments to evolve T7 RNA polymerase (RNAP) variants that either recognized the T3 promoter instead of T7 or initiated transcripts with ATP or CTP instead of GTP. (genengnews.com)
  • 87 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" 5'UTR 119. (novoprolabs.com)
  • 475) /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(493. (novoprolabs.com)
  • A possible role for RNA polymerase in the initiation of M13 DNA synthesis. (microbiologyresearch.org)
  • Many phage have proved useful in the study of molecular biology and as vectors for the transfer of genetic information between cells lambda bacteriophage can also undergo a lytic cycle or can enter a lysogenic cycle, in which the page DNA is incorporated into that of the host, awaiting a signal that initiates events leading to replication of the virus and lysis of the host cell. (genomicglossaries.com)
  • Bacteriophages may have a lytic cycle or a lysogenic cycle , but a few viruses are capable of carrying out both. (wikidoc.org)
  • Single-molecule assays centered around the "zinc fingered-phages" are being developed to establish M13 as a standalone, single-molecule building block for achieving universal connectivity and for unlocking future studies on the nature of protein-DNA interactions. (mit.edu)
  • It relies on relating the desired activity of a target protein with the fitness of an infectious bacteriophage which carries the protein's corresponding gene. (wikipedia.org)
  • Phage replication requires E. coli infection, which, for M13 phage, relies on protein III (pIII). (wikipedia.org)
  • 509) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind 517. (novoprolabs.com)
  • In addition to this technology, he has employed high-throughput screening platforms to investigate the function of genes, SNPs, and protein variants in mammalian cells. (wisc.edu)
  • Rather than making cuts in the genome, this modified version uses a special variant of the Cas9 protein that acts as a processor to read input delivered by guide RNA sequences, regulate the expression of a particular gene, and make a particular protein as an output. (richardvanhooijdonk.com)
  • Typically, bacteriophages consist of an outer protein hull enclosing genetic material . (wikidoc.org)
  • The three PACE experiments executed 45-200 rounds of evolution in 1.5-8 days and yielded T7 RNAP variants with activities on their target promoters or templates that exceeded or matched the activity of the wild-type enzyme transcribing the wild-type T7 promoter both in cells and in vitro," the team states. (genengnews.com)
  • 62 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" promoter 69. (novoprolabs.com)
  • Thus, we performed molecular dynamic (MD) simulations of laurusides-SARS-CoV-2 protease complexes to deepen the knowledge on the stability of the interaction and compare the effects of the targeting among the two genomic variants. (cnr.it)
  • This targeted installation of point mutations enables the identification and functional annotation of genetic variants including the dissection of mechanisms of drug resistance. (wisc.edu)
  • Mutagenizing Kumamolisin'''== Kunkel mutagenesis is a classic procedure for incorporating targeted mutations into a piece of DNA, so it was ideal for changing our wild-type Kumamolisin gene to code instead for specifically designed variant enzymes. (igem.org)
  • Overview of how Kunkel Mutagenesis works]] ==='''Kunkel Mutagenesis'''=== The first step to producing our specially designed enzymes was to change the wild-type gene that codes for Kumamolisin to code instead for variant enzymes with our desired amino acid substitutions. (igem.org)
  • We designed mutagenic oligonucleotide primers that would anneal to the wild-type Kumamolisin gene and incorporate point mutations that, when expressed, would result in a variant of Kumamolisin with the desired amino acid shift. (igem.org)
  • The lagoon contains M13 bacteriophage vectors carrying the gene of interest (known as the selection plasmid, or SP), as well as host E. coli cells that allow the phage to replicate. (wikipedia.org)
  • However, those who have both copies of the gene variant lay darker-colored eggs. (topvirallists.com)
  • To do this we infected cells with bacteriophage M13, which packages its own ssDNA genome identified by length, and so in tandem packaged our vector in single stranded form. (igem.org)
  • Cells were then infected with M13 helper phage (M13KO7, New Britain Biolabs, ratio bacterias: phage = 1:3) at 37 C for 20 min and phage-Fc creation was continued over night at 26 C, 230 rpm in 2YT/ampicillin/blood sugar with 0.5 mM IPTG and 30 g/ml kanamycin. (biomedigs.org)
  • This method was used to evolve Bacillus thuringiensis endotoxin variants that can overcome insect toxin resistance. (wikipedia.org)
  • A bacteriophage (from ' bacteria ' and Greek phagein , 'to eat') is any one of a number of viruses that infect bacteria. (wikidoc.org)
  • Bacteriophages are much smaller than the bacteria they destroy - usually between 20 and 200 nm in size. (wikidoc.org)
  • D'Hérelle called the virus a bacteriophage or bacteria-eater (from the Greek phagein meaning to eat). (wikidoc.org)
  • Using a virus known as M13 bacteriophage, the researchers were able to achieve a low-temperature construction of tiny germanium-tin-oxide wires and memory, which could enable future computers to reach speeds we can only dream about today. (richardvanhooijdonk.com)
  • Bacteriophage are viruses that infect bacterial hosts and can induce lysis upon infection. (sudoroom.org)
  • As such, M13 was further engineered to incorporate variants of the zinc finger, DNA-binding domain of transcription factor Zif268 as minor coat fusions. (mit.edu)
  • Whereas M13 mechanics were studied in the context of classical rod or polymer bending, our focus within kinesin mechanics is the molecular aspects of motility. (mit.edu)
  • The mut10 variant exhibited enhanced thermal stability, resistance to enzymatic degradation, and reduced aggregation in comparison to the original CH2 domain. (bvsalud.org)
  • Ultimately, our goal is to identify and characterize bacteriophage from wild type ARNG and use a composite cocktail of ARNG-associated phage as therapy to treat hosts infected with this pathogen. (sudoroom.org)
  • While this original PACE system only performed positive selection, a variant was developed that allowed for negative selection as well. (wikipedia.org)
  • A better understanding of its polymer mechanics is critical to the further development of future M13-based technologies. (mit.edu)
  • However, it was less stable than mut20 (L242C/K334C), which is the variant prepared in a previous study (Gong et al. (bvsalud.org)
  • In 2006 the UK Ministry of Defence took responsibility for a G8-funded Global Partnership Priority Eliava Project as a retrospective study to explore the potential of bacteriophages for the 21st century. (wikidoc.org)
  • BEZ235 Moreover, these IgG variants exhibited longer half-life in human FcRn transgenic mice. (biomedigs.org)
  • platform allowing effector function increase, our IgG variants retained both higher ADCC and higher FcRn binding. (biomedigs.org)
  • The lagoon contains M13 bacteriophage vectors carrying the gene of interest (known as the selection plasmid, or SP), as well as host E. coli cells that allow the phage to replicate. (wikipedia.org)
  • Phage replication requires E. coli infection, which, for M13 phage, relies on protein III (pIII). (wikipedia.org)
  • Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype. (stratech.co.uk)
  • Due to the different properties and advantages, Creative Biolabs is pleased to tailor the most appropriate phage display system (M13, T4 or T7) to meet our customers' demands. (stratech.co.uk)
  • Through DNA manipulation, numerous gene variants can be created and constructed as phage display library. (stratech.co.uk)
  • Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell. (expasy.org)
  • Ultimately, our goal is to identify and characterize bacteriophage from wild type ARNG and use a composite cocktail of ARNG-associated phage as therapy to treat hosts infected with this pathogen. (sudoroom.org)
  • Phage therapy is a biological therapy that uses bacteriophages (bacterial viruses) to infect and lyse bacterial pathogens. (sudoroom.org)
  • Phagemid cassette vector for the expression of functional recombinant single-chain Fv antibody - pIII fusion protein libraries on the surface of M13 filamentous phage using a E. coli lacI q genotype (e.g. (progen.com)
  • Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). (lookformedical.com)
  • BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. (lookformedical.com)
  • A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6. (lookformedical.com)
  • This method was used to evolve Bacillus thuringiensis endotoxin variants that can overcome insect toxin resistance. (wikipedia.org)
  • Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105. (lookformedical.com)
  • The application of lytic bacteriophages (phages) that show specificity towards malodour-producing species could therefore be selectively used to remove malodourous bacteria. (lpmhealthcare.com)
  • Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. (lookformedical.com)
  • Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. (lookformedical.com)
  • Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. (bvsalud.org)
  • The isolation of high affinity antibodies derived thereof was established by the expression of recombinant antibody fragments on the surface protein pIII of M13 bacteriophages, which can be rapidly screened against antigens bound to a solid phase. (progen.com)
  • The M13 bacteriophage protein pIII is located at one end of its tubular virion structure. (progen.com)
  • PROGEN now offers the phagemid cassette vector pSEX81 for the expression of functional single-chain Fv antibody - pIII fusion proteins on the surface of M13 bacteriophages. (progen.com)
  • The cassette vector pSEX81 was designed for the convenient insertion of heavy and light chain variable domain coding regions and for production of functional single-chain Fv antibody - pIII fusion proteins on the surface of M13 bacteriophages. (progen.com)
  • The amplified gene fragments encoding the variable heavy or light chain domain are cloned in-frame between a signal peptide sequence of bacterial pectate lyase (pelB) for the secretion of the fusion protein into the periplasmic space, and the pIII gene of M13 bacteriophage. (progen.com)
  • We showed the ability of two isolated bacteriophages to efficiently destroy biofilm formed by S. haemolyticus and S. lugdunensis which is one of the main bacterial species associated with production of body malodour. (lpmhealthcare.com)
  • BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. (lookformedical.com)
  • While this original PACE system only performed positive selection, a variant was developed that allowed for negative selection as well. (wikipedia.org)
  • A unique variant, C. neoformans var. (cdc.gov)