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  • acid
  • 05). (5) After EIPA inhibition, the rate of pH i recovery from the same acid load decreased to a similar value in both rat strains (0.0032±0.002 pH units/min in SHR and 0.0032±0.002 pH units/min in WKY rats). (ahajournals.org)
  • NaCl
  • Synchrotron SAXS data from solutions of Tryparedoxin K102E, reduced state in 10 mM HEPES pH 7.5, 50 mM NaCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). (sasbdb.org)
  • protein
  • The corresponding concentration was 0.82 g/L. Three independent determinations of the molecular mass were obtained from the value of I(0)/c, where c is the protein concentration, and using the programs SAXSMow2 and ScÅtter3 available at the URLs http://saxs.ifsc.usp.br/ and https://bl1231.als.lbl.gov/scatter/, respectively. (sasbdb.org)
  • The plot of a well-behaved folded protein approaches the baseline at high q values creating a parabolic shape. (bioisis.net)
  • assay
  • Each assay is repeated three times, and IC 50 values are determined graphically from plots of percent inhibition vs inhibitor concentration . (medchemexpress.com)
  • Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin and 10 μM [γ-TP at 30 °C. The kinase reaction was halted by adding 1 mL of 10% trichloroacetic acid. (apexbt.com)
  • level
  • The level of processing must be known both to compare values among studies and because each processing step may change the level of protein in oil). (ageofautism.com)
  • An extracellular answer was prepared with 5.4 mM KCl, 136 mM NaCl, 1 mM MgCl2, 1.8 mM CaCl2, 0.33 mM NaH2PO4, 10 mM glucose, 10 mM HEPES, and a pH level adjusted to 7.4 with NaOH and the osmolarity to 300 mosM. (health-ground.com)