AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentHealth Care
CoxiellaCoxiella burnetiiVacuolesQ FeverGoat DiseasesAntibodies, BacterialGoatsSheep DiseasesPhagosomesLysosomesMicroscopy, ElectronComplement Fixation TestsRickettsiaEndocarditis, BacterialRickettsia typhiAbortion, VeterinaryDNA, BacterialZoonosesRickettsia rickettsiiBlotting, SouthwesternLegionella pneumophilaVesicular Transport ProteinsSeroepidemiologic StudiesCathepsin ASaccharomyces cerevisiaeOrganoids
BurnetiiIntracellularChlamydiaInfectionMacrophageMaturationBacteriaAnaplasmaLeishmaniaGenusReplicationPromotesCellsLargeBurnetii is a gram-negativeReplicationBacterium that causesObligateParasitophorous vacuoleReplicatesLysosomalFeverEffector proteinsPathogenBacterial and host factorsVirulenceGenomeMolecularHostGreenResearch
Burnetii18
  • Vacuoles containing the intracellular pathogen Coxiella burnetii show reversible ESCRT recruitment, and interference with this recruitment reduces intravacuolar bacterial replication. (biorxiv.org)
  • Coxiella burnetii and Leishmania Mexicana Residing Within Similar Para" by Jess A. Millar, Raquel Valdés et al. (pdx.edu)
  • Coxiella burnetii is a bacterium that thrives in an acidic parasitophorous vacuole (PV) derived from lysosomes . (pdx.edu)
  • Coxiella burnetii is an obligate intracellular gram-negative bacterial pathogen, an ethiological agent of Q-fever, a zoonotic disease, elapsing as an acute (mostly atypical pneumonia) or a chronic (mostly endocarditis) form. (iimmun.ru)
  • C. burnetii promotes maturation of specific phagolysosome-like compartment in host cell, called coxiella-containing vacuole, within this vacuole pathogen becames metabolically activated and actively replicates. (iimmun.ru)
  • Except that C. burnetii involves autophagic pathway during coxiella-containing vacuole formation, and induction of autophagy promotes pathogen replication. (iimmun.ru)
  • Electron microscope image of a human cell vacuole where the bacterium that causes Q Fever (Coxiella burnetii) is busy growing. (iflscience.com)
  • Scholars@Duke publication: Lipid A Has Significance for Optimal Growth of Coxiella burnetii in Macrophage-Like THP-1 Cells and to a Lesser Extent in Axenic Media and Non-phagocytic Cells. (duke.edu)
  • Here we report the characterization of the role of lipid A in Coxiella burnetii growth in axenic media, monkey kidney cells (BGMK and Vero), and macrophage-like THP-1 cells by using a potent LpxC inhibitor -LPC-011. (duke.edu)
  • In conclusion, our data suggest that lipid A has significance for optimal development of Coxiella-containing vacuoles, and for robust multiplication of C. burnetii in macrophage-like THP-1 cells. (duke.edu)
  • In view of liver enzyme elevation and positive serology for Coxiella burnetii , the overall features are compatible with the clinical suspicion of Q fever associated hepatitis. (pathologyoutlines.com)
  • Coxiella burnetii - bacteria causing Q fever. (gutnews.com)
  • Some other pathogens that live inside cells include Brucella abortus , Listeria monocytogenes , Chlamydia trachomatis , Coxiella burnetii and Mycobacterium tuberculosis . (gutnews.com)
  • Q fever (see the image below) is a zoonosis caused by Coxiella burnetii, an obligate gram-negative intracellular bacterium. (medscape.com)
  • Recent success in the cultivation of Tropheryma whipplei [4] (which causes Wipple's disease) and Coxiella burnetii [5] (which causes Q fever) using genome-based metabolic pathway analyses has renewed interest in formulating axenic growth media for M. leprae . (internationaltextbookofleprosy.org)
  • PCR, immunohistochemistry, or culture of affected tissue can provide definitive confirmation of infection by Coxiella burnetii. (medscape.com)
  • in mice pretreated with an extract of coxiella burnetii. (liverpool.ac.uk)
  • mice injected intravenously with a commercially available extract of coxiella burnetii prepared for use as the antigen in the complement fixation diagnostic test for q fever were subsequently resistant to infection with babesia microti, babesia rodhaini, and plasmodium vinckei petteri. (liverpool.ac.uk)
Intracellular1
  • Collectively, this study identified host mRNAs and miRNAs that were influenced by Coxiella and/or Leishmania infections, and our data indicate that although their PVs are morphologically similar, Coxiella and Leishmania have evolved different strategies that perturb distinct host processes to create and thrive within their respective intracellular niches. (pdx.edu)
Chlamydia1
  • This exogenous KdtA enzyme modifies Coxiella lipid A with an α-Kdo-(2 → 8)-α-Kdo epitope that can be detected by anti-chlamydia genus antibodies. (duke.edu)
Infection2
  • Infection of THP-1 (human monocyte/macrophage) cells with Coxiella and Leishmania elicited disparate host responses. (pdx.edu)
  • Coxiella progenies prepared from inhibitor-treated cells retain the capability of normally infecting all tested cells in the absence of the inhibitor, which suggests a dispensable role of lipid A for infection and early vacuole development. (duke.edu)
Macrophage2
  • Bacteria have learned to survive when encased in a membrane-enclosed vacuole within the macrophage. (gutnews.com)
  • The researchers found that the bacteria which have infected a macrophage use weapons - virulence factors - to create holes in the membranes of both the vacuole and the macrophage. (gutnews.com)
Maturation2
  • After internalization of bacteria maturation of phagolysosome-like compartment and large coxiella-containing vacuole formation occure, and vacuole can occupy nearly the whole cytoplasm of the host cell. (iimmun.ru)
  • Its virulence depends on survival and replication properites in different cell types in which brucella controls the maturation of its vacuole to avoid innate immune responses and to reach its replicative niche associated with the endoplasmic reticulum. (mgc.ac.cn)
Bacteria4
  • Arrows indicate vacuoles containing bacteria. (cdc.gov)
  • Often starting as a phagosome in the endocytic compartment, these bacteria-occupied vacuoles transition into organelles with novel molecular features via remodeling of host proteins and lipids through the highly coordinated actions of bacterial factors [3] [5] . (microbialcell.com)
  • In times long past, highly evolved eukaryotes, for example biochemists, oc-ca-sio-nal-ly quipped that bacteria were nothing more than a bag of en-zymes. (asmblog.org)
  • Non‑digested bacteria were either released or established a replication niche within the con-tractile vacuole . (asmblog.org)
Anaplasma1
  • Romanowsky stained canine blood film containing Anaplasma platys (PCR confirmed), which are intraplatelet organisms within vacuoles. (ncvetp.org)
Leishmania2
  • As Coxiella and Leishmania are highly divergent organisms that cause different diseases, we reasoned that their respective infections would likely elicit distinct host responses despite producing phenotypically similar parasite-containing vacuoles. (pdx.edu)
  • Additionally, we detected 257 micro RNAs (miRNAs) that were expressed in THP-1 cells, and identified miRNAs that were specifically expressed during Coxiella or Leishmania infections. (pdx.edu)
Genus1
Replication1
  • Under inhibitor treatment, Coxiella has reduced growth yields in axenic media and during replication in non-phagocytic cells, and has a reduced number of productive vacuoles in such cells. (duke.edu)
Promotes1
  • From within a unique ER-derived vacuole L. pneumophila promotes host lipogenesis and experimental evidence indicates that cholesterol production might be one facet of this response. (microbialcell.com)
Cells2
  • In inhibitor-treated THP-1 cells, Coxiella shows severe growth defects characterized by poor vacuole formation and low growth yields. (duke.edu)
  • Large cell lymphoma and leukemia cells tend to have large size nuclei, less mature chromatin, and visible nucleoli with and without cytoplasmic vacuoles. (cytojournal.com)
Large1
  • In addition, cytoplasmic vacuoles are not uncommon in large cell lymphomas involving body cavities. (cytojournal.com)
Burnetii is a gram-negative2
  • Coxiella burnetii is a gram-negative intracellular bacterium that causes the human disease Q fever, which can present as an acute flu-like illness or persistent, focalized infection. (nih.gov)
  • Coxiella burnetii is a Gram-negative pathogen that infects a variety of mammalian hosts. (bvsalud.org)
Replication6
  • Here, it regulates vesicle trafficking pathways and fusion events to establish a large replication vacuole called the Coxiella -containing vacuole (CCV). (nih.gov)
  • 18. Noncanonical Inhibition of mTORC1 by Coxiella burnetii Promotes Replication within a Phagolysosome-Like Vacuole. (nih.gov)
  • In addition to LPS, the C. burnetii Dot/Icm type 4 secretion system (T4SS) is an important virulence determinant that delivers effector proteins required for the development of the Coxiella-containing vacuole and bacterial replication. (nih.gov)
  • Successful host infection requires replication of the pathogen within the lysosomal Coxiella-containing vacuole (CCV). (bvsalud.org)
  • Because PV biogenesis, host cell maintenance, and generation of developmental forms adapted to intracellular replication and extracellular resistance are central to Coxiella pathogenesis, we are conducting studies to better understand the molecular and cellular biology of these processes. (nih.gov)
  • The replication of A. phagocytophilum takes place within the vacuoles of neutrophil granulocytes and sometimes also lymphocytes [ 17 ] . (encyclopedia.pub)
Bacterium that causes2
  • Coxiella burnetii is an intracellular bacterium that causes query, or Q fever, a disease that typically manifests as a severe flu-like illness. (nih.gov)
  • We are investigating Coxiella burnetii , a bacterium that causes a severe flu-like illness called Q fever. (nih.gov)
Obligate4
  • Coxiella burnetii, the etiologic agent of Q fever, is a Gram-negative obligate intracellular bacterium. (uchile.cl)
  • Coxiella burnetii is an obligate zoonotic bacterium that targets macrophages causing a disease called Q fever. (bvsalud.org)
  • Rescue of Coxiella from an obligate intracellular lifestyle has enabled our development of complete set of genetic tools are now allowing fulfillment of molecular Koch's postulates for suspected Coxiella virulence genes. (nih.gov)
  • Q fever (see the image below) is a zoonosis caused by Coxiella burnetii, an obligate gram-negative intracellular bacterium. (medscape.com)
Parasitophorous vacuole3
  • The mechanisms the organism employs to cause disease are unclear but involve persistence in a parasitophorous vacuole and the subsequent host response. (tamu.edu)
  • It has been proposed that survival in the phagolysosomal-like parasitophorous vacuole requires specific iron uptake systems, secretion of enzymes to detoxify the compartment (catalase and SOD), and down-regulation of an oxidative burst (acid phosphatase). (tamu.edu)
  • During infection, the organism invades macrophages, where it directs formation of a lysosome-like parasitophorous vacuole (PV) in which to replicate. (nih.gov)
Replicates3
  • [ 4 ] Coxiella burnetii is taken up through the endocytic pathway and remains in an acidic vacuole where it replicates slowly. (medscape.com)
  • C. burnetii resides and replicates within the phagolysosomal vacuoles of macrophages and hence has a unique niche protected from the neutralizing effects of humoral immune response. (utsa.edu)
  • Like the ehrlichiae, the salmonid pathogen, designated strain LF-89, replicates within membrane-bound cytoplasmic vacuoles in host cells. (microbiologyresearch.org)
Lysosomal1
  • Coxiella has the extraordinary ability to replicate within a vacuole with lysosomal characteristics. (nih.gov)
Fever6
Effector proteins2
  • IMPORTANCE Coxiella burnetii secretes effector proteins via a T4BSS that are required for successful infection. (bvsalud.org)
  • Functional characterization of these effector proteins and their cellular targets will provide important insight into Coxiella virulence mechanisms. (nih.gov)
Pathogen3
  • Moreover, we are investigating the extent and relevance of Coxiella strain diversity and developing genetic methods to dissect the virulence of this refractory pathogen. (nih.gov)
  • In addition to providing needed information on pathogen biology, our research goals are aimed at development of new Coxiella countermeasures, such as rationally designed subunit vaccines and tools for molecular epidemiology. (nih.gov)
  • Our results indicate that modulation of host cell functions by Coxiella proteins is required for PV formation and pathogen growth. (nih.gov)
Bacterial and host factors1
  • Using contemporary cell biology techniques, we are characterizing the Coxiella PV to define both bacterial and host factors that mediate its formation. (nih.gov)
Virulence2
  • Comparative virulence of diverse Coxiella burnetii strains v. Virulence . (nih.gov)
  • Our comparative genomics studies revealed genetic heterogeneity among Coxiella strains and predicted strain-specific virulence factors. (nih.gov)
Genome2
  • Moreover, metabolic pathway reconstructions based on genome data helped us develop a medium that supports robust host cell-free (axenic) growth of Coxiella . (nih.gov)
  • Recombination between abundant insertion sequences (black vertical lines with triangle) contributes to Coxiella genome plasticity. (nih.gov)
Molecular1
Host1
  • This pseudo-colored transmission electron micrograph shows Coxiella burnetii infecting an African green monkey cell line, forming a large vacuole (green) in the host cell 8 days following primary inoculation. (nih.gov)
Green1
  • Pseudocolored scanning electron micrograph of a cryo-prepared Vero cell (orange) containing a PV filled with Coxiella (green). (nih.gov)
Research1
  • In 2019, Dr. Long received an Independent Research Scholar Award from NIH, allowing her to form an autonomous research group to continue her work on Coxiella burnetii . (nih.gov)