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  • synthase
  • Aspergillus nidulans has two α-1,3-glucan synthase genes, agsA and agsB . (frontiersin.org)
  • We previously revealed that AgsB is a major α-1,3-glucan synthase in vegetative hyphae, but the function of AgsA remained unknown because of its low expression level and lack of phenotypic alteration upon gene disruption. (frontiersin.org)
  • To clarify the role of α-1,3-glucan in hyphal aggregation, we constructed strains overexpressing agsA ( agsA OE ) or agsB ( agsB OE ), in which the other α-1,3-glucan synthase gene was disrupted. (frontiersin.org)
  • cells
  • α-1,3-Glucan of the agsB OE cells was clearly located in the outermost layer, whereas weak labeling was detected in the agsA OE cells. (frontiersin.org)
  • However, the agsA OE cells treated with β-1,3-glucanase were clearly labeled with AGBD-GFP. (frontiersin.org)
  • Sucrose hydrolysis is clearly a major physiological function of the Mn 1-encoded INCW2 in the BETL and there is evidence that metabolically released hexoses, not exogenous, are critical as a driving force in assimilate movement between maternal and filial cells ( Cheng and Chourey, 1999 ). (frontiersin.org)
  • wild-type
  • we performed a comparative large scale glycoproteome profiling of total proteins of these two genotypes ( mn 1 mutant vs. Mn 1 wild type) using 2D gel electrophoresis and glycosylation/total protein staining, followed by image analysis. (frontiersin.org)
  • cell wall
  • α-1,3-Glucan is one of the main polysaccharides in the cell wall of filamentous fungi. (frontiersin.org)
  • We also analyzed localization of α-1,3-glucan in the cell wall of the two strains by fluorescent labeling with α-1,3-glucan-binding domain - fused GFP (AGBD-GFP). (frontiersin.org)
  • Previous studies showed that basal developing endosperms of miniature 1 ( mn 1) mutant seeds lacking the Mn 1-encoded cell wall invertase II, are also deficient for hexose. (frontiersin.org)
  • The mn 1 mutation in maize is associated with a loss of 70% seed weight at maturity due to a loss-of-function mutation at the Mn 1 locus that codes for the endosperm-specific cell wall invertase 2 (INCW2). (frontiersin.org)