RecombinantGeneHuman ProteinsAssayConjugateBovine Serum AHomologyReceptorGrainsPeptidesResiduesReconstitutionAntigenAlbuminHeterozygousMembraneSerumTargeted nanoparticlesProteolytic cleavageGrain-freeEnzyme activityGenesAntibodiesSynthesisCoomassie BlueStabilityAqueousCatalogCellsAccumulationSterileTransporterMonomerSolubleTypeSubcellularCompositionMutationSequenceTargetsInteractAmino acidsEssential proteinCompoundsBindExpressionQuantitiesHighAttachmentIndigoidineImmunogenMethylPigment
Recombinant15
- The 154 amino acid recombinant protein has a predicted molecular mass of approximately 20.1 kD. (biolegend.com)
- BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. (biolegend.com)
- The 148 amino acid recombinant protein (with ADL-His9-SSGIEGR at N-terminus) has a predicted molecular mass of approximately 17 kD. (biolegend.com)
- Thus, endomembrane-modifying proteins within the endomembrane system will have an influence on the final grain quality/yield and recombinant protein production. (nature.com)
- Serial dilutions of Mouse IL-11 Recombinant Protein were added to B9 cells. (cellsignal.com)
- The purity of Mouse IL-11 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mIL-11 and staining with Coomassie Blue. (cellsignal.com)
- Mouse IL-11 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. (cellsignal.com)
- When Recombinant Human NETO1 His-tag Protein is immobilized at 4.00 µg/mL (100 µL/well), Recombinant Human APP695 +1 Fc Chimera binds with an ED 50 of 0.200-1.20 μg/mL. (bio-techne.com)
- Recombinant Human NETO1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Human NETO1 His-tag Protein (Catalog # 11103-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-48 kDa. (bio-techne.com)
- CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
- Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
- In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
- It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. (stem-immune.com)
- Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein Binding Activity Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10565-CV) binds Recombinant Human ACE-2 His-tag ( 933-ZN ) in a functional ELISA. (bio-techne.com)
- Recombinant SARS-CoV-2 Spike RBD Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant SARS-CoV-2 Spike RBD His-tag Protein (10565-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-60 kDa and 108-120 kDa, respectively. (bio-techne.com)
Gene4
- Blue squares indicate phenotypes directly attributed to mutations/alleles of this gene. (jax.org)
- Before this information can be used for protein synthesis, however, an RNA copy (transcript) of the gene must first be made. (khanacademy.org)
- The HTT gene produces huntingtin, a protein that's related to nerve cells in the brain. (healthline.com)
- meaning that mainly males are affected and females are carriers of the defective gene. (msdmanuals.com)
Human Proteins3
- Product Specifications Expression of Human Proteins in Human Cells Extreme low Endotoxin H. (stem-immune.com)
- Product Specifications Expression of Human Proteins in Human Cells Extreme low Endo. (stem-immune.com)
- Unless otherwise stated all data on this page refer to the human proteins. (guidetoimmunopharmacology.org)
Assay2
- Discover our full range of catalog products including Labeling and Detection, Peptides, Reagents for peptides synthesis, Assay Kits and Proteins. (anaspec.com)
- The assay is highly sensitive and specific for factor V Leiden, with excellent discrimination between normal individuals and factor V Leiden heterozygous carriers. (medscape.com)
Conjugate1
- Hapten-heterologous carrier conjugates elicited antihapten titers similar in magnitude to those elicited by the homologous carrier conjugate. (caltech.edu)
Bovine Serum A1
- Attempts were made to confer insolubility on protein antigens such as ovalbumin and crystalline bovine serum albumin without destroying their antigenicity. (caltech.edu)
Homology3
- Based on proteome annotation and a homology search, 94 proteins associated with the endomembrane system were identified that exhibited significant changes in abundance during grain development. (nature.com)
- 100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined. (novusbio.com)
- Although sequence homology is limited, crystallographic studies suggest conserved 3D structures across the group of binding proteins. (guidetoimmunopharmacology.org)
Receptor7
- A synthetic peptide from mouse 5HT1B receptor conjugated to blue carrier protein was used as the antigen. (osenses.com)
- The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. (osenses.com)
- Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). (bio-techne.com)
- The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. (rndsystems.com)
- The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). (bio-techne.com)
- Intersecting analysis, molecular docking, and pathway validation analysis showed that risperidone influences the adipocytokine signaling pathway by targeting MAPK14 (mitogen-activated protein kinase 14), MAPK8 (mitogen-activated protein kinase 8), and RXRA (retinoic acid receptor RXR-alpha), thereby inhibiting long-chain fatty acid β-oxidation by decreasing STAT3 (signal transducer and activator of transcription 3) expression and phosphorylation. (frontiersin.org)
- Dennis et al suggest that vesicle-associated membrane protein (VAMP7) mediates fusion of BLOC-1-dependent transport carriers with melanosomes and that soluble attachment protein receptor (SNARE) recycling from melanosomes is a critical BLOC-3-dependent step. (medscape.com)
Grains3
- By using label-free quantitative proteomics profiling, we quantified 1,822 proteins across developing barley grains. (nature.com)
- Packed with more of the chicken and fish cats love and free of the grains that contain gluten, BLUE Wilderness Wild Delights grain free wet cat food is made to feed your feline's wild side. (herald.ng)
- With recipes for all tastes and diets, including limited ingredient diets, high protein, grain-free, wholesome grains, and exotic proteins, BLUE Buffalo always starts with real meat, and ends with good health. (gotpetsupplies.com)
Peptides1
- In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into two distinct peptides, S1 and S2 subunits, is required for activation. (bio-techne.com)
Residues1
- The activated JAK proteins in turn phosphorylate specific residues on the IL-7R creating docking sites for signaling molecules such as STAT5 and to a lesser extent STAT1 and STAT3. (biolegend.com)
Reconstitution1
- Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. (biolegend.com)
Antigen4
- In a recent report by Garvey and Campbell (2) the loss of antigen material from hepatic tissue following a secondary injection of the native protein carrier was clearly demonstrated by radioautography. (caltech.edu)
- Passively administered homologous antibody did not produce a change in the retention of the primary injection of antigen nor did secondary injections of a heterologous native protein injected according to the same immunization schedule as the homologous azoprotein. (caltech.edu)
- 1) The general approach has been to attempt to produce an insoluble protein antigen which would combine specifically with antibody to give a complex that could be dissociated into soluble antibody and insoluble antigen, which could then be separated by centrifugation. (caltech.edu)
- However, in every case the resulting product either had lost too much native antigenicity (i.e., adsorbed serum always contained antibody which reacted with native antigen), was too soluble, or had too much power of adsorption for non-specific protein. (caltech.edu)
Albumin4
- The first is a simple pH indicator that turns blue if skin pH changes from five to nine, while the other two sensors focus on glucose to monitor blood sugar and the presence of protein carrier albumin. (medicaldevice-network.com)
- Meanwhile, the albumin sensor turns a lighter shade of green upon contact with the albumin protein. (medicaldevice-network.com)
- Albumin is a carrier and transport protein in the blood. (happydaze.io)
- The albumin sensor was based on a yellow dye that, upon association with the albumin protein, turned green. (happydaze.io)
Heterozygous4
- Heterozygous carriers of factor V Leiden typically have APCR ratios in the range of 1.5 -1.8. (medscape.com)
- The factor V Leiden mutation is not uncommon, with the heterozygous carrier state occurring in up to 5% of the US population. (medscape.com)
- All of the parents were heterozygous carriers of this mutation. (who.int)
- The high frequency of SSS and low frequency of consanguineous marriages in this study may reflect a high rate of heterozygous carriers. (who.int)
Membrane6
- NPT2 is an integral membrane protein expressed in kidney and lung. (thermofisher.com)
- single-pass type I membrane protein (Probable). (biosensis.com)
- This was also significant for being the first 3D crystal structure of any membrane protein complex. (goldenssport.com)
- Photosystem I is an integral membrane protein complex that uses light energy to catalyze the transfer of electrons across the thylakoid membrane from plastocyanin to ferredoxin. (goldenssport.com)
- SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein(S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). (bio-techne.com)
- SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. (bio-techne.com)
Serum2
- Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures. (caltech.edu)
- Elevated total serum calcium levels are associated with idiopathic hypercalcemia, vitamin D intoxication, hyperparathyroidism, sarcoidosis, pneumocystic carinii pneumonia, and blue diaper syndrome. (cdc.gov)
Targeted nanoparticles1
- In addition, proteomic profile confirmed that a set of proteins, related to tumor aggressiveness, were positively affected by targeted nanoparticles. (dovepress.com)
Proteolytic cleavage1
- Activated protein C (APC) is the enzymatically active form of protein C after proteolytic cleavage by thrombomodulin-bound thrombin. (medscape.com)
Grain-free1
- And unlike conventional grain-free dog food, 2/3 of the animal proteins in this premium dog food are fresh or raw, so each bite is bursting with nutritional goodness and taste. (ssb-woo.com)
Enzyme activity1
- Further experiments used a mutant form of the TERT protein that cannot interact with other TERT molecules and found that complexes that contain this mutant protein still have normal enzyme activity. (elifesciences.org)
Genes8
- In most gram-positive bacteria, this operon consists of five genes dltXABCD but the involvement of the first ORF ( dltX ) encoding a small protein of unknown function, has never been investigated. (frontiersin.org)
- The genetic study showed promising results in "silencing" the genes that translate into tau protein, the primary component behind dementia. (medicaldaily.com)
- Many genes encode protein products, meaning that they specify the sequence of amino acids used to build a particular protein. (khanacademy.org)
- Importantly, not all genes encode protein products. (khanacademy.org)
- For instance, some genes specify ribosomal RNAs ( rRNAs ), which serve as structural components of ribosomes, or transfer RNAs ( tRNAs ), cloverleaf-shaped RNA molecules that bring amino acids to the ribosome for protein synthesis. (khanacademy.org)
- Still other RNA molecules, such as tiny microRNAs ( miRNAs ), act as regulators of other genes, and new types of non-protein-coding RNAs are being discovered all the time. (khanacademy.org)
- X-Linked Inheritance Genes are segments of deoxyribonucleic acid (DNA) that contain the code for a specific protein that functions in one or more types of cells in the body or code for functional RNA molecules. (msdmanuals.com)
- 20 000 protein coding genes (Parsons mutations. (who.int)
Antibodies2
- BIOSONDA's trade marks include BLUE CARRIER, a top selling carrier protein obtained from the moluskConcholepas concholepasfor the production of antibodies. (biosonda.com)
- The problem of obtaining from precipitation experiments evidence about the structure of antibodies and the nature of serological reactions is obviously greatly simplified by the replacement of protein antigens by simple substances of known structure. (caltech.edu)
Synthesis1
- This final product is a blue pigment called indigoidine, and its synthesis from two molecules of L-glutamine is powered by ATP. (wgtn.ac.nz)
Coomassie Blue3
- 97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. (novusbio.com)
- Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. (anticorps-enligne.fr)
- 95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. (novusbio.com)
Stability1
- The two investigated osmolytes have opposite effects on protein stability but have virtually the same effect on water dynamics, suggesting that they do not act indirectly via solvent perturbations. (lu.se)
Aqueous1
- Fatty acid-binding proteins are low molecular weight (100-130 aa) chaperones for long chain fatty acids, fatty acyl CoA esters, eicosanoids, retinols, retinoic acids and related metabolites and are usually regarded as being responsible for allowing the otherwise hydrophobic ligands to be mobile in aqueous media. (guidetoimmunopharmacology.org)
Catalog2
- Catalog # NL007 ) and counterstained with DAPI (blue). (rndsystems.com)
- Catalog # CTS002 ) and counterstained with hematoxylin (blue). (rndsystems.com)
Cells6
- The "cargo carriers," or endosomes, of certain brain cancer cells contain overactive NHE9 proteins (blue), which pump out too many protons (orange), changing the endosomes' acidity and slowing their "shipping speed. (medicaldaily.com)
- DNA bears the hereditary information that's passed on from parents to children, providing instructions for how (and when) to make the many proteins needed to build and maintain functioning cells, tissues, and organisms. (khanacademy.org)
- The cells were counter stained with Hoechst Dye ( blue colour). (biosensis.com)
- Therefore, a future challenge will be to refine the method to allow experiments to use much less protein, which would more closely reflect how telomerase is produced in cells. (elifesciences.org)
- ATP is the major carrier of energy to all cells. (nutrimedical.com)
- Hemoglobin is the protein contained in red blood cells that enables them to carry oxygen from the lungs and deliver it to all parts of the body. (msdmanuals.com)
Accumulation4
- Cereal endosperm is a short-lived tissue adapted for nutrient storage, containing specialized organelles, such as protein bodies (PBs) and protein storage vacuoles (PSVs), for the accumulation of storage proteins. (nature.com)
- Together our data identified promising targets to be genetically engineered to modulate seed storage protein accumulation that have a growing role in health and nutritional issues. (nature.com)
- By further integrated proteomics and metabolomics analysis, we found that protein expression and metabolite levels changed significantly in the liver and lungs of mice due to the high accumulation of PB NPs, leading to slight inflammatory responses and intracellular oxidative stress. (biomedcentral.com)
- Conversely, it is thought to protect against several neurodegenerative diseases that are caused by the accumulation of misfolded proteins (11,12). (novusbio.com)
Sterile1
- Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)
Transporter1
- The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
Monomer2
- BpsA contains an adenylation domain that recognises and sequentially binds two molecules of L-glutamine, an oxidation domain that is believed to oxidise each glutamine monomer, a peptidyl carrier protein domain that binds the phosphopantetheine moiety, and a thioesterase domain that cyclises each glutamine and releases the final bicyclic product from the enzyme. (wgtn.ac.nz)
- The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). (bio-techne.com)
Soluble2
- In the bacterial reaction center, the electron is obtained from a reduced compound haem group in a cytochrome subunit or from a water-soluble cytochrome-c protein. (goldenssport.com)
- Like many Chinese medicinal herbs, licorice root has high content of soluble proteins, some of which are glycated and remain soluble even in the boiling decoction. (springer.com)
Type3
- Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. (rndsystems.com)
- This type of RNA is called a messenger RNA ( mRNA ), as it serves as a messenger between DNA and the ribosomes, molecular machines that read mRNA sequences and use them to build proteins. (khanacademy.org)
- Type I photosystems use ferredoxin-like iron-sulfur cluster proteins as terminal electron acceptors, while type II photosystems ultimately shuttle electrons to a quinone terminal electron acceptor. (goldenssport.com)
Subcellular1
- notably, integration of proteomics data with in situ subcellular microscopic analyses showed a high abundance of cytoskeleton proteins associated with acidified PBs at the early development stages. (nature.com)
Composition2
- Depending on the protein trafficking, PB composition is affected, thereby influencing the qualitative output. (nature.com)
- When exposed to biological medium or multiple composition dispersions like herbal decoction, protein corona formed on the nanoparticles (NPs) diversely alters the biological fate and pharmaceutical efficacy of particles [ 10 ]. (springer.com)
Mutation2
- For example, in northwestern Puerto Rico, the prevalence is 1 in 1800, with 1 in 22 being carriers of the mutation. (medscape.com)
- IDH1 We identified IDH1 mutations in 5 mutations were co-present with TP53 astrocytomas that developed in carriers mutations in 63% of low-grade diffuse of a TP53 germline mutation. (who.int)
Sequence1
- The peptide is homologous with the corresponding sequence derived from APG7 protein in mouse, rat, S. cerevisiae, Macaca mulatta (monkey) and Canis familiaris (dog). (biosensis.com)
Targets1
- 4'-Phosphopantetheinyl transferases (PPTases) play an essential role in activating the carrier protein domains of mega-synthases involved in primary and secondary metabolism and have been validated as promising drug targets in multiple pathogens. (bvsalud.org)
Interact2
- The ephedrine alkaloid-containing colloidal nanoparticles discovered in another licorice containing Chinese medicinal decoction [ 12 ] imply that licorice root proteins would hypothetically interact with aconitine to form aggregates thereafter affecting the toxicity. (springer.com)
- However, if either of these processes proceeds too slowly or fails, HSP70/HSPA1A can interact with the HSP40 co-chaperone protein and the CHIP/STUB1 Ubiquitin ligase (E3) to promote ubiquitination and degradation of the nascent polypeptide or denatured protein (3,4). (novusbio.com)
Amino acids3
- Chicken and trout provide readily digestible protein and essential amino acids. (herald.ng)
- Newly produced antibody, like the other plasma proteins, became readily labelled with S35 amino acids within a few hours. (caltech.edu)
- High-quality proteins and essential amino acids from aquatic animals act as carrier substances for the natural color intensifier. (baybridgeaquarium.com)
Essential protein2
- Collagen is the essential protein used to repair damaged tissue and to replace old tissue. (nutrimedical.com)
- We found that sphingomyelin is enriched in migrasomes and identified sphingomyelin synthase 2 (SMS2) as an essential protein for migrasome biogenesis. (bvsalud.org)
Compounds2
- It has been known that liquiritin from licorice forms complexation with aconitine and therefore reduces the amount of free aconitine [ 7 ], indicating that interactions between aconitine with other major amphiphilic compounds (i.e., protein) from licorice may facilitate the formation of aconitine complex of such kind, too. (springer.com)
- This perspective focuses on two areas that have yielded new useful information during the last 20 years: (i) structure-activity relationship (SAR) studies of contact allergy based on the concept of hapten-protein binding and (ii) mechanistic investigations regarding activation of nonsensitizing compounds to contact allergens by air oxidation or skin metabolism. (cdc.gov)
Bind2
- However, many enzymes can bind to different combinations of proteins to form groups (or 'complexes') with a variety of three-dimensional shapes, so there may be a variety of enzyme complexes in the solution. (elifesciences.org)
- To cause sensitization, a chemical must bind to macromolecules (proteins) in the skin. (cdc.gov)
Expression2
- There was also an upregulation in expression of positive regulators and key components of the AMPK pathway, autophagy, proteasome function, and the unfolded protein response. (nature.com)
- Risperidone upregulates fatty acid synthase (FASN) and sterol regulatory element-binding protein 1 (SREBP1) expression in hepatocyte cultures and mouse liver by targeting the hepatic SREBP-1c/FASN couple, which is also one of the mechanisms by which risperidone induces weight gain ( 24 ). (frontiersin.org)
Quantities1
- Large quantities of purified proteins were used in this study. (elifesciences.org)
High4
- Proteomics study identified 88 high stringent differentially expressed proteins comparing the three treatment groups with controls. (dovepress.com)
- Featuring real chicken and trout, plus fruits and vegetables, this high protein cat food features the finest natural ingredients enhanced with vitamins and minerals. (herald.ng)
- Ultimately, the electrons that are transferred by Photosystem I are used to produce the high energy carrier NADPH. (goldenssport.com)
- You will select one (recommended) or two (if you are able to support twins) embryos for implantation in a carrier of your choice: you, your partner, a surrogate, or an artificial womb housed in our high-security incubation facility. (marketplace.org)
Attachment1
- For functionality, NRPSs require the attachment of a phosphopantetheine moiety to their peptidyl carrier protein domains. (wgtn.ac.nz)
Indigoidine1
- Monitoring phosphopantetheinylation of the non-ribosomal peptidase synthetase BpsA, which produces blue indigoidine pigment upon activation, is a useful strategy to screen chemical collections for inhibitors of a target PPTase. (bvsalud.org)
Immunogen1
- Immunogen Description A synthetic peptide (DSTRDRTLDQQC) corresponding to the C-terminal of human APG7 protein conjugated to blue carrier protein has been used as the immunogen. (biosensis.com)
Methyl2
- The first sensor was a rather simple pH indicator consisting of the dyes methyl red, bromothymol blue, and phenolphthalein. (happydaze.io)
- A protein solution with the properties of a specific antiserum to the triphenylmethane dye methyl blue has been made by treating a solution of bovine gamma-globulin and the dye with alkali and then slowly neutralizing the alkali. (caltech.edu)
Pigment1
- The NRPS blue pigment synthetase A (BpsA) is unusual in that it is comprised of only a single module. (wgtn.ac.nz)