Uropathogenic escherichia adhesins escherichia coli. Medical search. Frequent questions

The effectiveness of anti-adhesin antibodies is illustrated by studies with FimH, the adhesin of uropathogenic Escherichia coli (UPEC). (wikipedia.org)
Here, we report an efficient gene editing strategy based on direct delivery of Cas9 and gRNA into Uropathogenic E. coli (UPEC) isolate. (microbiologyresearch.org)
Uropathogenic Escherichia coli (UPEC) is responsible for the majority of urinary tract infections (UTI). (pasteur.fr)
Another group of adhesins that contributes to UPEC-mediated UTI is autotransporter (AT) proteins. (pasteur.fr)
One recently characterized AT protein of UPEC is UpaH, a large adhesin-involved-in-diffuse-adherence (AIDA-I)-type AT protein that contributes to biofilm formation and bladder colonization. (pasteur.fr)
Urinary tract infections (UTIs), mostly caused by uropathogenic E. coli (UPEC), are important human infections. (clinmedjournals.org)
Uropathogenic E. coli (UPEC) are endowed with several virulence associated factors (VFs) that enable the organism to attach, invade, and injure the host. (clinmedjournals.org)
The human urinary tract is one of the most common sites of bacterial infection and most of them are caused by uropathogenic Escherichia coli (UPEC). (esrf.fr)
Uropathogenic Escherichia coli (UPEC) attach specifically to human bladder cells using surface structures known as type 1 pili. (esrf.fr)
Greater than 85% of UTI are caused by uropathogenic Escherichia coli (UPEC). (justia.com)
Animal models of UTI have revealed that the mannose-binding FimH adhesin of type 1 pili is critical for the colonization of and invasion into the bladder epithelium by UPEC, as well as other uropathogenic members of the Enterobacteriaceae family, such as Klebsiella, Enterobacter , and Citrobacter species. (justia.com)

The best characterized bacterial adhesin is the type 1 fimbrial FimH adhesin. (wikipedia.org)
The first subunit to be engaged is FimH, the adhesin at the very tip of the pilus, and subsequent subunits are added one at a time from the base up. (esrf.fr)
The adhesin subunit FimH is in green (FimH L and FimH p stand for the lectin and the pilin domains respectively) and the periplasmic chaperone FimC is in yellow. (esrf.fr)
The distal FimH adhesin protein is connected to the the pilus rod by the flexible tip fibrillum, which is composed of one copy each of FimF and FimG. (justia.com)
The adhesin tip protein FimH is a two-domain protein comprised of a pilin domain (FimHp), which allows it to incorporate into the pilus, and a lectin domain (FimHL) that contains a conserved mannose binding pocket. (justia.com)
In all of these models the adhesin FimH has been shown to play an integral role in pathogenesis, highlighting it as an excellent therapeutic target. (justia.com)
The first step in the colonization of the human urinary tract by pathogenic Escherichia coli is the mannose-sensitive binding of FimH, the adhesin present at the tip of type 1 pili, to the bladder epithelium. (rcsb.org)
Our structure/function analysis demonstrated that the binding of the monosaccharide alpha-D-mannose is the primary bladder cell receptor for uropathogenic E. coli and that this event requires a highly conserved FimH binding pocket. (rcsb.org)
The residues in the FimH mannose-binding pocket were sequenced and found to be invariant in over 200 uropathogenic strains of E. coli. (rcsb.org)
Only enterohaemorrhagic E. coli (EHEC) possess a sequence variation within the mannose-binding pocket of FimH, suggesting a naturally occurring mechanism of attenuation in EHEC bacteria that would prevent them from being targeted to the urinary tract. (rcsb.org)
These studies are devoted to the structure/functional analysis of allelic variations of the E. coli type 1 fimbrial adhesin, FimH protein. (washington.edu)

Biochemical characteristic of biofilm of uropathogenic Escherichia coli Dr+ strains. (mostwiedzy.pl)
Antimicrobial resistant strains of Escherichia coli ( E. coli ) are a common cause of UTI which were identified as a treatment challenge. (biomedcentral.com)
Southern blot analysis of the S fimbrial adhesin (sfa) determinants of normal and hyper-fimbriated strains revealed no marked difference in the gene structure. (uni-wuerzburg.de)
The second project is focused on identification of the 'selection footprints' of nucleotide polymorphisms that affect various types of genes encoding different adhesins of E. coli, Shigella and Salmonella strains. (washington.edu)
Moreover, the array was highly sufficient to investigate the gene content of non-pathogenic isolates, despite the strong bias towards pathogenic E. coli strains that have been sequenced so far. (biomedcentral.com)
This high-density microarray provides an excellent tool for characterizing the genetic makeup of unknown E. coli strains and can also deliver insights into phylogenetic relationships. (biomedcentral.com)
Yet, most of what we know about E. coli stems from the investigation of laboratory strains, which fail to capture the ecology and evolution of this key organism "in the wild" ( Hobman, Penn & Pallen, 2007 ). (peerj.com)

This adhesin is responsible for D-mannose sensitive adhesion. (wikipedia.org)
The first project is directed towards understanding the mechanism of bacterial adhesion to host cells mediated by the most common kind of bacterial adhesins known - type 1, or mannose-sensitive, fimbriae. (washington.edu)
The effect of D-mannose and D-glucose on bacteriuria due to Escherichia coli with mannose-sensitive adhesins was investigated in adult male Sprague-Dawley rats undergoing diuresis. (dmanna.com)

These factors were evaluated in various phylogenetic groups (phylotypes) of E. coli isolates. (biomedcentral.com)
The ability of extended spectrum β-lactamases (ESBLs) production in E. coli isolates was detected using the combined disc diffusion method. (biomedcentral.com)
This study's results confirmed an explosion of antibiotic resistance amongst E. coli isolates from UTI against β-lactams and fluoroquinolones. (biomedcentral.com)
However, despite this staggering statistic, very few studies have investigated the relationship between biofilm production and host factors such age group and hospitalization status, and non-host factors, including recurrence of UTI, phylogenetic group distribution, and antibiotic susceptibility, among urinary E. coli isolates from reproductive age women. (clinmedjournals.org)
Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates: PCR assays for detection of Afa adhesins that do or do not recognize Dr blood group antigens. (childrensmercy.org)
In 31 clinical isolates of E. coli tested, C3-dependent internalisation was evident in 10 isolates. (biomedcentral.com)
For the identification and subtyping of Escherichia coli isolates, a wide variety of typing methods have been developed. (biomedcentral.com)
A microarray chip approximating the complete pan-genome of E. coli would provide optimal sensitivity to characterize isolates. (biomedcentral.com)
We isolated and whole-genome sequenced up to five colonies of faecal E. coli from 66 asymptomatic children aged three-to-five years in rural Gambia (n = 88 isolates from 21 positive stools). (peerj.com)
Several isolates encode putative virulence factors commonly found in Enteropathogenic and Enteroaggregative E. coli, and 53% of the isolates encode resistance to three or more classes of antimicrobials. (peerj.com)

Adhesins are expressed by both pathogenic bacteria and saprophytic bacteria. (wikipedia.org)
Numerous studies have shown that inhibiting a single adhesin in this coordinated effort can often be enough to make a pathogenic bacterium non-virulent. (wikipedia.org)
Hence, we conclude that CQD-CRISPR-Cas9-gRNA could be a novel therapeutic strategy for the eradication or treatment of antibiotic resistant pathogenic E. coli -based pathologies. (microbiologyresearch.org)

Bacterial virulence factors, especially fimbrial adhesins, have been conclusively shown to promote host cell invasion. (biomedcentral.com)
E. coli encode a variety of virulence factors that facilitate colonisation of the urinary tract, such as fimbrial adhesins (type 1, P, S, and Dr fimbriae) and toxins (α-hemolysin and cytotoxic necrotising factor 1 (CNF1)) [ 7 ]. (biomedcentral.com)
In addition to pathogen virulence factors, complement C3 secreted by host cells also influences the ability of E. coli to invade cells and tissues within the urinary tract. (biomedcentral.com)

In most UTI cases, E. coli is the causative pathogen, with over 80-90% of uncomplicated UTIs caused by this organism [ 3 ]. (clinmedjournals.org)
Analysis of the variability of S fimbriae expression in an Escherichia coli pathogen. (uni-wuerzburg.de)
Bacterial infection of the urinary tract is a common clinical problem with E. coli being the most common urinary pathogen. (biomedcentral.com)

Most fimbria of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. (wikipedia.org)
In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. (wikipedia.org)
The bacterial adhesion consists primarily of an intramembranous structural protein which provides a scaffold upon which several extracellular adhesins may be attached. (wikipedia.org)
Vaccinating mice against a key protein that E. coli use to latch onto the bladder and cause UTIs reduces severe disease, according to researchers at Washington University School of Medicine in St. Louis. (wustl.edu)
They found that E. coli lacking FmlH were less likely to establish chronic infections in mice than bacteria with the protein. (wustl.edu)
Our recent study reported that C3 opsonisation markedly increases the ability of E. coli strain J96 to internalise into human proximal tubular epithelial cells via CD46, a complement regulatory protein expressed on host cell membrane. (biomedcentral.com)
Moreover, C3 opsonisation promotes E. coli invasion of human uro-epithelial cells via CD46, a complement regulatory protein expressed on host cell membranes [ 13 ]. (biomedcentral.com)

A large number of bacterial adhesins with individual receptor specificities have been identified. (wikipedia.org)
Some of these variations result in adaptive changes of the receptor-specificity of type 1 fimbriae that lead to an increased tissue tropism and, consecutively, virulence of uropathogenic and meningitis-associated E. coli. (washington.edu)
we propose that efficient internalisation of uropathogenic E. coli by the human urinary tract depends on co-operation between type 1 fimbriae-mediated adhesion and C3 receptor -ligand interaction. (biomedcentral.com)

In this study, we established a dynamic micromodel of urinary tract infection to analyze the impact of UT-segment-specific urinary outflow on the persistence of E. coli colonization. (mostwiedzy.pl)
Urinary tract infection (UTI) due to uropathogenic E. coli is a common clinical problem, estimated to affect 40-50% of women at least once in their lifetime [ 1 ]. (biomedcentral.com)

About 80 percent of UTIs are caused by Escherichia coli (E. coli) . (wustl.edu)

This function is mediated primarily by fimbrial adhesins, which mediate specific attachment to host cell receptors. (pasteur.fr)
Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell line. (childrensmercy.org)

Using 32 Escherichia coli and Shigella genome sequences we estimate the pan- and core genome of the species. (biomedcentral.com)
Here, we determine an approximate E. coli pan-genome, based on 24 E. coli and 8 Shigella genomes available at the time of analysis (November 2006). (biomedcentral.com)

Erythrocyte and porcine intestinal glycosphingolipids recognized by F4 fimbriae of enterotoxigenic Escherichia coli. (expasy.org)

Bacterial adhesins provide species and tissue tropism. (wikipedia.org)

Heptyl α-d-mannose prevents binding of type 1-piliated E. coli to the human bladder cell line 5637 and reduces both adhesion and invasion of the UTI89 cystitis isolate instilled in mouse bladder via catheterization. (dmanna.com)

Investigating the role of epithelial surface architecture, we showed that the presence of budding cells-a model microarchitectural obstacle-promotes colonization of the urinary tract by E. coli. (mostwiedzy.pl)
We report a previously undescribed phenomenon of epithelial cell "rolling-shedding" colonization, in which the detached epithelial cells reattach to the underlying cell line through a layer of adherent Dr+ E. coli. (mostwiedzy.pl)

Expression of these adhesins at different phases during infection play the most important role in adhesion based virulence. (wikipedia.org)
This has led to the exploration of adhesin activity interruption as a method of bacterial infection treatment. (wikipedia.org)
Additionally, Adhesins are attractive vaccine candidates because they are often essential to infection and are surface-located, making them readily accessible to antibodies. (wikipedia.org)
Researchers have identified a potential way to prevent chronic urinary tract infections, a common infection primarily caused by E. coli (shown above). (wustl.edu)
Then, they infected both groups with E. coli and measured the amount of bacteria in their bladders and kidneys one, two and three days after infection. (wustl.edu)
Studies from our group have shown that mice deficient in C3 are resistant to ascending infection and complement can alter bacterial uptake by mouse proximal tubular epithelial cells (PTECs), a primary target of E. coli during the acute phase of pyelonephritis [ 12 ]. (biomedcentral.com)
Little is known about the genomic diversity of Escherichia coli in healthy children from sub-Saharan Africa, even though this is pertinent to understanding bacterial evolution and ecology and their role in infection. (peerj.com)

Adhesins are cell-surface components or appendages of bacteria that facilitate adhesion or adherence to other cells or to surfaces, usually in the host they are infecting or living in. (wikipedia.org)
In the crudest sense, bacterial adhesins serve as anchors allowing bacteria to overcome these environmental shear forces, thus remaining in their desired environment. (wikipedia.org)
To effectively achieve adherence to host surfaces, many bacteria produce multiple adherence factors called adhesins. (wikipedia.org)
The study of adhesins as a point of exploitation for vaccines comes from early studies which indicated that an important component of protective immunity against certain bacteria came from an ability to prevent adhesin binding. (wikipedia.org)
It was demonstrated by itrtmunofluorescence microscopy that in noimal (wild-type) and hyperS- fimbriated E. coli populaiions non-fimbriated cells also · exist, and that the percentage of Sfinibrlated and non-fimbriated bacteria was roughly identica1 in either population. (uni-wuerzburg.de)
The researchers removed the gene for FmlH from E. coli and then infected the urinary tracts of mice with bacteria with and without the gene. (wustl.edu)
Oral therapy with an empirically chosen antibiotic that is effective against gram-negative aerobic coliform bacteria (eg, Escherichia coli ) is the principal treatment intervention in patients with cystitis. (medscape.com)

To avoid being swept away during urination, E. coli attaches to the bladder with long, hairlike projections called pili. (wustl.edu)

We designed a high-density microarray in order to provide a tool for characterization of the E. coli pan-genome. (biomedcentral.com)

Also, this study adds significantly to the number of commensal E. coli genomes, a group that has been traditionally underrepresented in the sequencing of this species. (peerj.com)
Although clinical microbiology typically relies on single-colony picks (which has the potential to underestimate species diversity and transmission events), within-host diversity of E. coli in the gut is crucial to our understanding of inter-strain competition and co-operation and also for accurate diagnosis and epidemiological analyses. (peerj.com)

Glycoconjugate receptors for P-fimbriated Escherichia coli in the mouse. (expasy.org)

E. coli strain J96 (serotype O4: K6) was provided by Dr. R. Welch, (University of Wisconsin, Madison, USA). (biomedcentral.com)
Technical performance of this pan-genome microarray based on control strain samples ( E. coli K-12 and O157:H7) demonstrated a high sensitivity and relatively low false positive rate. (biomedcentral.com)

UTI, although treatable, is now becoming increasingly tough to control because of rampant antimicrobial resistance in the Enterobacteriaceae family, particularly inEscherichia coli. (microbiologyresearch.org)

Many of these infections are initiated by a critical interaction between host ligands (frequently polysaccharide moieties) and bacterial adhesins (frequently expressed at the distal tip of polymeric pilus fibers assembled by the chaperone-usher pathway). (justia.com)

P-fimbriae plays major role in bacterial adherence to the uroepithelium through the Galα1-4Gal-binding PapG adhesin. (microbiologyresearch.org)
We found that the adherence of Dr+ E. coli to bladder T24 transitional cells and type IV collagen is maximal at lowest shear stress and is reduced by any increase in flow velocity. (mostwiedzy.pl)

Work with E. coli stems from observations of human acquired immunity. (wikipedia.org)

Adhesins are a type of virulence factor. (wikipedia.org)
This basic structure is conserved across type 1 fimbrial adhesins though recent studies have shown that in vitro induced mutations can lead to the addition of C-terminal domain specificity resulting in a bacterial adhesion with dual bending sites and related binding phenotypes. (wikipedia.org)

In this study, we further assessed whether C3-dependent internalisation by human tubular epithelial cells is a general feature of uropathogenic E. coli and investigated features of the bacterial phenotype that may account for any heterogeneity. (biomedcentral.com)

Thus, resident E. coli in these children may constitute reservoirs of virulence- and resistance-associated genes. (peerj.com)

Adhesion and bacterial adhesins are also a potential target for prophylaxis or treatment of bacterial infections. (wikipedia.org)

Many bacterial pathogens are able to express an array of different adhesins. (wikipedia.org)

Furthermore, the sequence analysis of the VgrG proteins, proposed to be exported in a T6SS-dependent way, confirmed that some C-terminal regions possess domains showing similarities with adhesins or proteins with enzymatic functions. (biomedcentral.com)

His postdoctoral research involved structure/functional analysis of the Escherichia coli fimbrial adhesins at the University of Tennessee, Memphis. (washington.edu)

Once Dr+ E. coli bound to host cells or collagen, they did not detach even in the presence of elevated shear stress or of chloramphenicol, a competitive inhibitor of binding. (mostwiedzy.pl)
These were spread over 37 seven-allele sequence types and the E. coli phylogroups A, B1, B2, C, D, E, F and Escherichia cryptic clade I. Immigration events accounted for three-quarters of the diversity within our study population, while one-quarter of variants appeared to have arisen from within-host evolution. (peerj.com)

CD46 was not involved in the binding of E. coli to epithelial cells. (biomedcentral.com)

Anatomy18

Organisms27

Diseases12

Chemicals and Drugs97

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes77

Disciplines and Occupations2

Technology, Industry, Agriculture1

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UPEC11

FimH11

Strains7

Mannose-sensitive3

Isolates10

Pathogenic3

Virulence factors3

Pathogen3

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Receptor3

Urinary tract inf2

UTIs1

Mediate2

Shigella2

Intestinal1

Tissue tropism1

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Colonization2

Infection7

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Pili1

Characterization1

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Pilus1

Adherence2

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