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  • methods
  • However, the methods and materials presented here can also be used for other injection procedures in drug delivery to the brain, spinal cord or other organs in small mammals. (jove.com)
  • It is our goal to describe these methods in a highly accessible manner so that the procedures can be successfully performed in research laboratories even just with basic cell biology setup. (jove.com)
  • mice
  • Here, we describe our protocols for standardizing the bacterial inoculum 7 as well as the procedure for catheterizing anesthetized male mice for instillation of bacteria. (jove.com)
  • The protocol describes the procedures to induce telomere dysfunction and reactivate telomerase activity in mTERT-ER and LSL-mTERT mice in vivo . (jove.com)
  • The protocol also describes the procedures used to isolate neural stem cells (NSCs) from mTERT-ER and LSL-mTERT mice and reactivate telomerase activity in NSCs in vitro . (jove.com)
  • kits
  • Several commercial versions of this procedure have been developed however, the commercial kits are optimized for RhoA GTPase and typically do not work well for RhoC GTPase. (jove.com)
  • primary
  • The following protocols describe our procedures for the dissociation and culturing of primary human samples to enrich for BTIC populations, as well as the dissociation of tumorspheres. (jove.com)
  • cells
  • This article delineates the procedures involved in this assay, including sample preparation, intravenous injection, and culturing cells from lung metastases. (jove.com)
  • Finally, the protocol describes the procedures for performing telomere FISH (Fluorescence In Situ Hybridization) on both mouse FFPE (Formalin Fixed and Paraffin Embedded) brain tissues and metaphase chromosomes of cultured cells. (jove.com)