Urine polymerase chain reaction. Medical search. Frequent questions

A comparison of reverse-transcription polymerase chain reaction (RT-PCR) test results for urine and serum specimens from 66 persons with Zika virus disease with both specimens collected on the same date indicated that approximately twice as many urine specimens (61) than serum specimens (31) tested positive. (cdc.gov)
On the basis of previous small case studies reporting real time reverse-transcription polymerase chain reaction (RT-PCR) detection of Zika virus RNA in urine, saliva, and semen ( 2 - 6 ), the Florida Department of Health collected multiple specimen types from persons with suspected Zika virus disease. (cdc.gov)
Real-time reverse transcription-polymerase chain reaction (rRT-PCR) is the preferred test for Zika virus infection because it can be performed rapidly and is highly specific ( 1 , 2 ). (cdc.gov)
The EUAs authorize real-time reverse transcription-polymerase chain reaction (rRT-PCR) assays to detect Zika virus RNA in specified clinical sample types, and an immunoglobulin M (IgM) antibody capture enzyme-linked immunosorbent assay (ELISA) to detect anti-Zika virus IgM antibodies in serum and cerebrospinal fluid. (cdc.gov)

Materials and Methods: We developed a highly specific and sensitive nested methylation specific polymerase chain reaction assay to detect the presence of bladder cancer in small volumes of patient urine. (tau.ac.il)
Conclusions: We developed a novel methylation specific polymerase chain reaction assay for the detection and monitoring of bladder cancer using DNA extracted from patient urine. (tau.ac.il)
Currently, the CDC Trioplex rRT-PCR assay is the only diagnostic tool authorized by the Food and Drug Administration for Zika virus testing of urine ( 1 ). (cdc.gov)
A polymerase chain reaction (PCR)-based multiplex dual probe assay, the test for chlamydia and gonorrhea offers excellent sensitivity and high specificity and runs on the automated cobas 4800 System, complementing the cobas HPV (Human Papillomavirus)Test that received FDA approval in April. (news-medical.net)
The CDC Trioplex rRT-PCR assay is authorized by FDA for Zika virus testing of urine and serum. (cdc.gov)
Perform Zika virus rRT-PCR testing on urine, in conjunction with testing of serum using the appropriate molecular or serologic assay, based on days post symptom onset. (cdc.gov)

Metal concentrations were measured using inductively coupled plasma mass spectrometry (ICP-MS). TL was measured by quantitative real-time polymerase chain reaction (PCR). (nih.gov)

We used real-time polymerase chain reaction (PCR) to detect L. pneumophila DNA in peripheral blood and serum samples and urine antigen from a patient with pneumonia. (dovepress.com)
Regarding confirmation studies by real-time polymerase chain reaction, the urine sample had significantly higher positivity values than the serum samples from the onset of the clinical picture. (bvsalud.org)

s-IP permeability was assayed by high-performance liquid chromatography determination in the urine of the lactulose to mannitol ratio. (hindawi.com)

A further comparison of 53 persons with Zika virus disease with urine, saliva, and serum specimens collected on the same date found positive results from testing in 49 (92%) urine specimens, 43 (81%) saliva specimens, and 27 (51%) serum specimens. (cdc.gov)
RT-PCR was routinely performed on urine, serum, or saliva specimens collected within 21 days of symptom onset. (cdc.gov)
Further investigation is needed to determine the sensitivity and utility of Zika virus rRT-PCR on urine specimens collected ≥14 days after onset of symptoms. (cdc.gov)
CDC now recommends that, for persons with suspected Zika virus disease, Zika virus rRT-PCR should be performed on both urine and serum specimens collected within 7 days after onset of symptoms. (cdc.gov)
Zika virus rRT-PCR also should be performed on urine specimens collected within 14 days after onset of symptoms. (cdc.gov)

The regions surrounding the DNA methylation sites were amplified in a methylation independent first round polymerase chain reaction and the amplification product from the first polymerase chain reaction was used in a real-time methylation specific polymerase chain reaction. (tau.ac.il)
The development of DNA amplification techniques such as polymerase chain reaction (PCR) has focused the attention of investigators toward this direction. (scielo.org.mx)
A polymerase chain reaction (PCR) test is an example of a nucleic acid amplification test. (healthlinkbc.ca)
Telomerase activity was determined using polymerase chain reaction, which refers to the telomeric repeat amplification protocol. (medicaljournal-ias.org)

Her nasopharyngeal polymerase chain reaction test for the COVID-19 RNA virus was negative. (frontiersin.org)

Laboratory testing of urine samples now can be performed with dipsticks that indicate immune system responses to infection, as well as with microscopic analysis of samples. (wikipedia.org)
While urine electrolysis requires further research in the laboratory, struvite precipitation and nitrification/distillation have already been operated at pilot scale. (scielo.org.za)
Thus, the IQ-based safety threshold of urine fluoride levels that we identified in the subgroup TT may be considered as an initial testing value to be refined at multilevels of the laboratory, epidemiological, and clinical studies. (fluoridealert.org)
Polymerase chain reaction (PCR) is a revolutionary laboratory technique that enables the replication of a specific DNA sequence. (news-medical.net)
The main interest was to optimize the laboratory procedures and to test the hypothesis that urine and plasma concentrations reflect each other. (biomedcentral.com)

On May 13, 2016, the Centers for Disease Control and Prevention (CDC) issued interim guidance ( https://www.cdc.gov/mmwr/volumes/65/wr/mm6518e1.htm ) that recommends Zika virus rRT-PCR testing of urine collected less than 14 days after symptom onset, along with testing of patient-matched serum samples, for the diagnosis of suspected Zika virus infection (1). (cdc.gov)

2000) Polymerase chain reaction in clinically suspected genitourinary tuberculosis: comparision with intravenous urography, bladder biopsy, and urine acid fast bacilli culture. (medscape.org)

Comparison of Zika virus testing in serum, urine and saliva specimens from travel-associated Zika virus disease cases-Florida, 2016. (cdc.gov)

Zika virus RNA has been found in the serum, saliva, urine, semen and vaginal secretions of infected individuals. (canada.ca)
Paul S. Mead, MD, MPH, an epidemiologist at the Centers for Disease Control and Prevention in Fort Collins, Colorado, and colleagues prospectively studied ZIKV shedding in semen (n = 184) and urine (n = 183) of men with symptomatic ZIKV infection. (medscape.com)
Samples were collected twice per month, and the analysis included 1327 semen samples and 1038 urine samples obtained 14 to 304 days after illness onset. (medscape.com)

However, it is difficult to detect because of the limited amount of DNA present in the urine of patients with bladder cancer. (tau.ac.il)
citation needed] In immunocompromised patients, pus is present in the urine but often no organism can be cultured. (wikipedia.org)

Analyze and interpret results from tests, such as microbial or parasite tests, urine analyses, hormonal assays, fine needle aspirations (FNAs), and polymerase chain reactions (PCRs). (onetonline.org)

Recent reports using adaptations of previously published methods ( 2 , 4 ) suggest that Zika virus RNA can be detected in urine for at least 2 weeks after onset of symptoms ( 3 , 5 - 7 ). (cdc.gov)
Although Zika virus RNA is unlikely to be detected in serum after the first week of illness, recent data suggest that Zika virus RNA can persist in urine for at least two weeks post symptom onset (3). (cdc.gov)

Limited data suggest Zika virus is excreted in multiple body fluids, including urine and saliva. (cdc.gov)
Procedures for the collection and submission of body fluids, including urine specimens, have been described previously ( 9 ). (cdc.gov)

Humans can get Leptospirosis through contact with the urine of an infected animal, but because there are only 100 to 200 cases per year in the US, the CDC does not even consider it a reportable disease. (dogsnaturallymagazine.com)

Discussion on various supply chain members such as manufacturers, suppliers, service providers and diagnostic centres. (bccresearch.com)
QIAGEN N.V. today announced that the U.S. Food and Drug Administration (FDA) has granted two 510(k) clearances for its real-time PCR (polymerase chain reaction) instrument Rotor-Gene Q MDx and a compatible test for the detection of Influenza A/B, the artus Infl A/B RG RT-PCR Kit, for in vitro diagnostic use. (news-medical.net)

Zika virus detection in urine from patients with Guillain-Barré syndrome on Martinique, January 2016. (cdc.gov)
Of upcoming interest is hypermethylated DNA (hmDNA)-based cancer detection in urine. (utwente.nl)
The specific hmDNA gene was only detected in the hmDNA-enriched DNA from the urine of a cervical cancer patient and not in the control sample, thereby providing proof-of-principle for hmDNA-based cervical cancer detection in urine using the hmDNA enrichment device. (utwente.nl)

Urine antigen testing has not been studied sufficiently. (medscape.com)

Chlamydia tests use a sample of body fluid or urine to see whether chlamydia bacteria ( Chlamydia trachomatis ) are present and causing an infection. (healthlinkbc.ca)
Dogs can catch Leptospira bacteria from water or soil that's contaminated with infected urine from rodents and other wild animals. (dogsnaturallymagazine.com)

However, some patients may have bacterial infection despite negative urine cultures. (medscape.com)
Doctors diagnose the infection by identifying the virus in a sample of urine, saliva, blood, or tissue. (msdmanuals.com)
During his hospital evaluation for septic shock he was noted to be febrile, hypotensive, profoundly jaundiced with aseptic meningitis, and renal failure, and was diagnosed with Leptospirosis interrogans infection confirmed by serum and urine polymerase chain reaction. (ajtmh.org)
Methods: Blood and urine samples were obtained from the patient and tested for evidence of Lassa fever virus infection. (cdc.gov)
However, a negative rRT-PCR in a serum or urine sample collected at any time point after illness onset does not exclude Zika virus infection, and in these cases IgM antibody testing should be performed on serum. (cdc.gov)
Collect urine samples within 14 days post symptom onset along with patient-matched serum samples for those who match CDC Zika virus clinical and/or epidemiological testing criteria for Zika virus infection. (cdc.gov)

As urine is easily attainable, bladder cancer is an optimal cancer to detect using DNA methylation. (tau.ac.il)
In children, polymerase chain reaction sequencing of urine can detect fragments of the infectious agent. (wikipedia.org)

Main outcome measures Ribonucleic acid (RNA) viral load measured in respiratory, stool, serum, and urine samples. (bmj.com)

The symptoms of hemorrhagic cystitis include pain or burning feeling while urinating, frequent need to urinate, loss of control of the bladder, blood or blood clots in the urine, inability to urinate, and fever. (wikipedia.org)
The presence of hematuria, or blood in the urine, may indicate acute UTIs, kidney disease, kidney stones, inflammation of the prostate (in men), endometriosis (in women), or cancer of the urinary tract. (wikipedia.org)
In some cases, blood in the urine results from athletic training, particularly in runners. (wikipedia.org)
These tests look for the measles or mumps virus in a sample of your blood, fluid from your nose or throat, or urine. (medlineplus.gov)
this was followed by a progressive elevation of blood pressure, gross hematuria, and decreased urine levels 2 weeks later. (frontiersin.org)
Pyuria is a urinary condition characterized by a high number of white blood cells in the urine . (wikidoc.org)
It is generally characterized as the presence of 10 or more white blood cells per cubic millimetre in a sample of urine , 3 or more white cells per high-power field of unspun urine, a positive result on Gram's staining of an unspun urine specimen or a urinary dipstick test that is positive for leukocyte esterase . (wikidoc.org)
Polymerase chain reaction-restriction fragment length polymorphism methods were used to genotype DRD2 Taq 1A polymorphism with genomic DNA isolated from whole blood collected at the enrollment. (fluoridealert.org)
However, no direct correlation between miRNA expression levels in blood and urine has been yet clearly demonstrated. (biomedcentral.com)
Blood samples tested positive for Lassa fever RNA by reverse transcriptase polymerase chain reaction. (cdc.gov)
Urine and blood samples were collected to test for HIV and select STIs. (who.int)

The aim of this study was to isolate three members of miR-200 family from tumor tissue, plasma and urine of high-grade serous ovarian cancer patients in comparison with samples from patients with benign ovarian tumors. (biomedcentral.com)
Tumor tissue and plasma, but not urine analysis was able to discriminate malignant and benign samples. (biomedcentral.com)

Analysis of urine specimens and prostatic fluid is used to confirm the diagnosis (see Workup). (medscape.com)

To evaluate the effectiveness of nested polymerase chain reaction (PCR) for diagnosis of extrapulmonary tuberculosis (ETB), as well as the impact of PCR results on clinical management. (scielo.org.mx)
PCR for rapid diagnosis of extrapulmonary TB has an adequate effect, which improves when performed on urine. (scielo.org.mx)
2000) Rapid diagnosis of genitourinary tuberculosis by polymerase chain reaction and non-radioactive DNA hybridization. (medscape.org)

Mead and colleagues also found that only 1% of urine samples had ZIKV RNA, and none had infectious virus. (medscape.com)

Scientists also have found no evidence that HIV is spread through sweat, tears, urine, or feces. (diagnose-me.com)

HIV nucleic acid sequences have been detected in urine by polymerase chain reaction (13). (cdc.gov)

Evaluar la eficacia de la reacción en cadena de la polimerasa (PCR) anidada para el diagnóstico de tuberculosis extrapulmonar, así como el impacto de sus resultados en el manejo clínico. (scielo.org.mx)

PCR: The detection of CMV DNA was performed with polymerase chain reaction (PCR) targeting the envelope glycoprotein gB and gH genes by Vries et al. (cdc.gov)

A chlamydia test is done on either a urine sample or fluid (direct sample) collected from the area of the body that is most likely to be infected. (healthlinkbc.ca)
OTCQX: RHHBY) announced today that the U.S. Food and Drug Administration (FDA) has provided 510(k) clearance to the cobas® CT/NG Test for the detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) infections in both symptomatic and asymptomatic patients from male urine and self -collected vaginal swabs. (news-medical.net)

Urine specimens were collected from 70 patients with suspected Zika virus disease from zero to 20 days after symptom onset. (cdc.gov)
In the majority of cases, the urine samples were very useful from the first days after the onset of the symptoms. (bvsalud.org)

The registrational trial for the cobas CT/NG test also confirmed that self-collected vaginal specimens and male urine specimens provide increased sensitivity and specificity when compared with alternative specimen types across patient populations with both low and high disease prevalence. (news-medical.net)

These results suggest urine might be a useful specimen for identifying acute Zika virus disease. (cdc.gov)
BPHL testing results suggest urine might be the preferred specimen type to identify acute Zika virus disease. (cdc.gov)

This test can also be done on a urine sample. (healthlinkbc.ca)
If your test is being done on a urine sample, do not urinate for 2 hours before the sample is collected. (healthlinkbc.ca)
There is no discomfort in collecting a urine sample. (healthlinkbc.ca)
A doctor may collect a urine sample, throat swab, or rectal swab to test for gonorrhea . (healthline.com)
Plasma, but also urine sample could represent a simple liquid biopsy for ovarian cancer biomarker detection. (biomedcentral.com)

A PCR (polymerase chain reaction) test is a common type of molecular test. (medlineplus.gov)
This test will contribute to lowering the burden of disease by providing accurate results from easy to obtain samples - self-collected vaginal swabs from women and first catch urine from males. (news-medical.net)
Spun urine can be used as a screening test but it is less sensitive (64% of those with positive cultures) than vaginal fluid exam (73% of those with positive cultures), but both used together pick up about 85% of those who are culture positive. (wdxcyber.com)

In 2010 the international and trans-disciplinary research project VUNA was initiated in order to explore technologies and management methods for better urine management in eThekwini. (scielo.org.za)
Thirty three voided urine samples were gathered from patients, which were previously identified as bladder carcinoma cases using cytological methods. (medicaljournal-ias.org)

Collect the first part of your urine stream, just as you start to urinate. (healthlinkbc.ca)

Male urine and self-collected vaginal swabs are the preferred specimen types for CT and NG testing, according to the summary report from the U.S. Association of Public Health Laboratories and Centers for Disease Control and Prevention. (news-medical.net)

Urinalysis and urine culture may be ordered when indicated. (medscape.com)

Urine samples were collected from patients receiving treatment at Wolfson Medical Center in Holon, Israel. (tau.ac.il)
Schmidt BL, Aberer E, Stockenhuber C, Klade H, Breier F, Luger A. Detection of Borrelia burgdorferi DNA by polymerase chain reaction in the urine and breast milk of patients with Lyme borreliosis. (healingwell.com)

In the present work, we analyzed aberrations in G-banded chromosomes, reproductive hormone levels, and polymerase chain reaction-based V(D)J rearrangement frequencies in applicators whose exposures were mostly limited to chlorophenoxy herbicides. (cdc.gov)

41% (8383) responded by sending in urine and questionnaire. (bmj.com)

results from testing nine of 11 urine specimens were positive. (cdc.gov)

Multiple linear regression models were applied to evaluate the relationship between urine fluoride levels and IQ scores overall and within the DRD2 Taq 1A SNP = CC/CT and TT subgroups. (fluoridealert.org)

Urine fluoride had a stronger association with IQ in children with TT genotype. (fluoridealert.org)
The threshold of urine fluoride affecting IQ in children with TT genotype existed. (fluoridealert.org)
Urine fluoride levels had a stronger association with IQ in children carrying the TT genotype (adjusted ß = - 12.31, bootstrapped standard error (SE) = 1.28), compared to that in overall participants (adjusted ß = - 2.47, bootstrapped SE = 3.75) (Z = 2.483 and bootstrapped p = 0.007). (fluoridealert.org)

A safety threshold of urine fluoride levels for IQ impairment was determined in the subgroup TT. (fluoridealert.org)
We also determined a safety threshold of urine fluoride on intelligence impairment in the subgroup TT as 1.73 mg/L urine fluoride with a 95% CI of (1.51 mg/L, 1.97 mg/L). According to WHO Drinking water Quality 4th edition (2011), fluoride in drinking water quality standard is 1.5 mg/L (WHO, 2011). (fluoridealert.org)
To date, no national or international intelligence impairment-based safety threshold of urine fluoride levels (an internal exposure index) is available. (fluoridealert.org)

Affected reservoir species experience chronic colonization of proximal renal tubules and shed leptospires in urine. (medscape.com)

Anatomy18

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Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes82

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Anthropology, Education, Sociology and Social Phenomena1

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Specimens collected5

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Semen3

Present in the urine2

Assays1

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Body fluids2

Humans1

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Detection in urine3

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