Urine by high performance liquid chromatography. Medical search. Frequent questions

The sample undergoes high performance liquid chromatography, mass spectrometry or immunoassay. (home-remedies-for-you.com)
Zhang Y, Wang Q, Zhang G, Jia W, Ren Y, Wu Y. Biomarker analysis of hemoglobin adducts of acrylamide and glycidamide enantiomers for mid-term internal exposure assessment by isotope dilution ultra-high-performance liquid chromatography tandem mass spectrometry. (cdc.gov)
Liquid-liquid extraction was followed by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. (ojp.gov)
Unexpected results from urine immunoassays should have a confirmatory gas chromatography-mass spectrometry or a high-performance liquid chromatography test performed. (uspharmacist.com)
Caffeine and 14 of its metabolites are quantified in urine by use of high-performance liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry (HPLC-ESI-MS/MS) with stable isotope labeled internal standards. (cdc.gov)
The test principle utilizes high performance liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry (HPLC-APPI-MS/MS) for the quantitative detection of genistein, daidzein, equol, O-desmethylangolensin, enterodiol, and enterolactone. (cdc.gov)
Gas chromatography-mass spectrometry (GC-MS) was used for confirmation. (erowid.org)
Methods are mainly based on gas chromatography and (tandem) mass spectrometry, high performance liquid chromatography as well as atomic absorption spectroscopy. (dguv.de)
Marek EM, Koslitz S, Weiß T , Fartasch M, Schlüter G, Käfferlein HU, Brüning T. Quantification of N-phenyl-2-naphthylamine by gas chromatography and isotope-dilution mass spectrometry and its percutaneous absorption ex vivo under workplace conditions. (dguv.de)
Title : Two-dimensional high performance liquid chromatography separation and tandem mass spectrometry detection of atrazine and its metabolic and hydrolysis products in urine☆ Personal Author(s) : Kuklenyik, Zsuzsanna;Panuwet, Parinya;Jayatilaka, Nayana K.;Pirkle, James L.;Calafat, Antonia M. (cdc.gov)
The investigators used high-performance liquid chromatography-tandem mass spectrometry of urine and serum to detect nonadherence to antihypertensive medications among 1348 patients with hypertension in the UK and Czech Republic. (pharmacytimes.com)
To fill this gap, the investigators developed the liquid chromatography-tandem mass spectrometry to assess for nonadherence to antihypertensive drugs in blood or urine. (pharmacytimes.com)
The results revealed that the petroleum ether-soluble fraction of Angelicae Sinensis Radix exhibited significant anticoagulant activity in vitro, and 26 compounds were characterized by high-performance liquid chromatography with diode array detection combined with electrospray ionization ion trap time-of-flight multistage mass spectrometry. (unboundmedicine.com)
The analyses were performed using ultra-high performance liquid chromatography coupled to high resolution Q Exactive(TM) Orbitrap(TM) mass spectrometry (UHPLC-HRMS). (uni-koeln.de)
At the baseline, spot urine samples were collected and seven urinary flavonoid metabolites were quantified using ultra-performance liquid chromatography coupled to triple quadrupole mass spectrometry (UPLC-Q-q-Q MS). Liver enzymes, inflammatory scores, and urinary flavonoid concentrations were inverse normally transformed. (unav.edu)
Urinary PYR metabolite levels were quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). (aaem.pl)
Urine biomarkers of triclosan (TCS), triclocarban (TCC), parabens and benzophenone-3, bisphenols and dichlorophenols (DCP) were quantified by mass spectrometry. (biomedcentral.com)
In addition to assessing genital and systemic safety, we determined TFV concentrations in plasma and cervicovaginal fluid (CVF) and LNG levels in serum using tandem liquid chromatography-mass spectrometry. (cdc.gov)
Determination of colchicine in biological fluids by liquid chromatography mass spectrometry with pneumatically assisted electrospray ionization. (cdc.gov)

1999) using gas chromatography (GC), high performance liquid chromatography (HPLC) (Buchholz et al. (cdc.gov)
Phytoestrogens are then separated from other urine components by reversed phase HPLC, detected by APPI-MS/MS, and quantified by isotope dilution. (cdc.gov)
MDMA and MDA were determined in plasma and urine by reversed-phase high-performance liquid chromatography with diode array detection (HPLC-DAD) after solid-phase extraction on cation-exchange columns. (erowid.org)
To investigate the elimination kinetics of psilocin (PI), the first metabolite of PY, urine was collected for 24 h and PI concentrations were determined by high-performance liquid chromatography with column switching and electrochemical detection (HPLC-ECD). (erowid.org)
Confirmed diagnosis of sickle cell disease (SCD) (e.g. any genotype including HbSS, HbSC, HbSß0-thalassemia, HbSß+-thalassemia, and others) by hemoglobin electrophoresis or high-performance liquid chromatography (HPLC) performed locally. (who.int)

Human metabolism of pyrethroids occurs rapidly, and metabolites are eliminated in urine and bile over several days. (cdc.gov)
Human metabolism of these organophosphorus insecticides results in the specific metabolites named above, dialkylphosphate metabolites, and other metabolites, all which are eliminated in urine over a few days. (cdc.gov)
Detecting any of these urinary metabolites in a person's urine may indicate exposure to the specific organophosphorus insecticide or to the environmental breakdown product or both. (cdc.gov)
This method can be used to assess human exposure to select non-persistent pesticides by measuring their metabolites in urine. (cdc.gov)
A sensitive analytical method was developed for oxycodone and its metabolites, noroxycodone and oxymorphone, in human plasma, urine (±enzymatic hydrolysis at 50°C for 16 h) and liver microsomes (HLMs). (ojp.gov)
Particular focus is on the development of validated and quality controlled analytical methods for the determination of hazardous substances, their metabolites or their interaction products with macromolecules in blood and urine samples of humans. (dguv.de)
Ulrich N, Bury D, Koch HM, Rüther M, Weber T, Käfferlein HU, Weiß T , Brüning T, Kolossa-Gehring M. Metabolites of the alkyl pyrrolidone solvents NMP and NEP in 24-h urine samples of the German Environmental Specimen Bank from 1991 to 2014. (dguv.de)
In addition, 5 prototype constituents, 24 in vivo metabolites in rat urine and 7 prototype constituents, and 9 in vitro metabolites in the rat hepatic S9 incubation system of the petroleum ether-soluble fraction were tentatively identified. (unboundmedicine.com)
A total of 21 metabolites were tentatively identified from the in vivo experiments, of which several novel glucuronides were detected in plasma and urine. (uni-koeln.de)
In hydrolyzed urine, hydroxylated metabolites dominated. (uni-koeln.de)
In urine, the suggested targets are two monohydroxylated metabolites after hydrolysis with beta-glucuronidase, selected both due to prolongation of the detection time and the availability of reference material from the in vitro models. (uni-koeln.de)
Our aim was to investigate the potential association between specific urine flavonoid metabolites, liver enzymes, and inflammatory status in individuals with metabolic syndrome (MetS). (unav.edu)
Conclusion: This cross-sectional study showed that higher urinary excretion of citrus-flavanone metabolites was associated with lower GGT levels in subjects diagnosed with MetS and obesity. (unav.edu)
The study also found that the proportion of these urinary metabolites varied considerably depending on whether the subjects had high or low acute exposures or whether the subjects had general environmental exposures (i.e., not agricultural workers). (who.int)

s-IP permeability was assayed by high-performance liquid chromatography determination in the urine of the lactulose to mannitol ratio. (hindawi.com)
Determination of methylhippuric acid in human urine by high-performance liquid chromatography and by isotachophoresis. (cdc.gov)
Tracqui A, Kintz P, Ludes B, Rouge C, Douibi H, Mangin P. High-performance liquid chromatography coupled to ion spray spectrometry for the determination of colchicine at ppb levels in human biofluids. (cdc.gov)
Determination of the antimitotic agents N-Desacetylcolchicine, demecocline and colchicine in serum and urine. (cdc.gov)

Initial testing includes porphobilinogen (PBG) measurement, urine porphyrin fractionation, and erythrocyte protoporphyrin measurements. (arupconsult.com)

2004). The target analytes are extracted and concentrated from the urine matrix using an automated solid phase extraction system. (cdc.gov)

Patients may also drink an excessive amount of water (2-4 qt) or use diuretics to purposefully dilute their urine and the urine drug concentration to decrease the chance of detection. (uspharmacist.com)
This selective method allows for rapid detection of phytoestrogens in human urine with limits of detection in the low parts per billion (ng/mL) range. (cdc.gov)
Detection of the urine biomarkers varied according to chemical type and demographics. (biomedcentral.com)

Diagnosis is based on elevated levels of the porphyrin precursors delta- aminolevulinic acid and porphobilinogen in the urine during attacks. (msdmanuals.com)

We conclude that in humans PI is partially excreted as PI-O-glucuronide and that enzymatic hydrolysis extends the time of detectability for PI in urine samples. (erowid.org)

Except for the presence of high serum unconjugated bilirubin levels, the results of liver tests in Crigler-Najjar syndrome type 1 are normal. (medscape.com)
Conjugated bilirubin is absent from serum, and bilirubin is not present in urine. (medscape.com)
A case in which colchicine is detected in urine, serum, or plasma (2), as determined by a laboratory. (cdc.gov)

La spectrométrie de masse en tandem, les technologies moléculaires et l'analyse biochimique ont été utilisées pour les besoins du dépistage. (who.int)

A 50-µL aliquot of urine is first diluted with 450 µL of water. (cdc.gov)

The majority of urine samples (98.8%) contained one or more PYR metabolite, although only a few women self-reported pesticide exposure. (aaem.pl)

As appropriate, provide the required urine collection container and specimen collection instructions. (unboundmedicine.com)

For urine analysis, urine samples can be extracted with diethyl ether. (cdc.gov)
Human urine samples are processed using enzymatic deconjugation of the glucuronidated phytoestrogens followed by size-exclusion filtration. (cdc.gov)
Plasma and urine samples were collected from two patients in a controlled clinical study over periods of 9 and 22 h, respectively. (erowid.org)
Peak PI concentrations up to 870 microg/l were measured in urine samples from the 2-4 h collection interval. (erowid.org)
Addition of beta-glucuronidase to urine samples and incubation for 5 h at 40 degrees C led to twofold higher PI concentrations, although 18+/-7% of the amount of unconjugated PI was decomposed during incubation. (erowid.org)
Methods: Urine samples were collected from 152 volunteer smokers (75 cigarette and 77 HB) as well as from 16 healthy controls. (who.int)
Oral administration of ACP-105 was performed in horses, where blood and urine samples were collected over a time period of 96 h. (uni-koeln.de)
Demographics, clinical variables, and self-report of personal care products use along with urine samples were collected concurrently from 496 adults (48% females, median age: 28 years) and 90 adolescents (10-17 years of age) from the RHINESSA study in Bergen, Norway. (biomedcentral.com)
Cranberry fruit samples harvested from open oligotrophic wetland habitats contained higher levels of anthocyanins, anthocyanidins, flavonol glycosides, and proanthocyanidins. (bvsalud.org)
Sera and urine specimens, green mussel and seawater samples were tested for saxitoxin levels using high performance liquid chromatography. (who.int)

We aimed to assess the associations of use of personal care products with urine biomarkers levels of phenols and paraben exposure, and whether urine levels (reflecting body burden of this chemical exposures) are associated with eczema, rhinitis, asthma, specific IgE and body mass index. (biomedcentral.com)

High-throughput, simultaneous quantitation of hemoglobin adducts of acrylamide, glycidamide, and ethylene oxide using UHPLC-MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. (cdc.gov)
Quantitation is based on peak area ratios interpolated against an 11-point calibration curve derived from calibrators in synthetic urine. (cdc.gov)

Selective separation of the analytes is achieved using high-performance liquid chromatography with a gradient elution program. (cdc.gov)

Urine specimens are processed, stored, and shipped to the Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention for analysis. (cdc.gov)

However, the levels of 3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DBCA) and 4-fluoro-3-phenoxybenzoic acid (4F3PBA) were higher than those reported in previous studies. (aaem.pl)
This neurotoxin is water-soluble, acid stable and relatively heat stable even in high temperature. (who.int)

Analytes exhibited room temperature stability in plasma and urine up to 24 h, and freeze-thaw stability in plasma up to three cycles. (ojp.gov)

Reported results for all assays meet the Division of Environmental Health Laboratory Sciences' quality control and quality assurance performance criteria for accuracy and precision (similar to specifications outlined by Westgard (1981). (cdc.gov)

The calibration range was 0.2-250 ng/mL for plasma and HLM and 10-5000 ng/mL for urine. (ojp.gov)
Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. (erowid.org)

Definitive diagnosis of Crigler-Najjar syndrome requires high-performance liquid chromatography of bile or a tissue enzyme assay of a liver biopsy sample. (medscape.com)

1996), and urine (Ikonen et al. (cdc.gov)

Urine is diagnosed in the laboratory for the type of infection. (home-remedies-for-you.com)
Providing a sample of urine, blood, saliva, hair or any other fluid to a laboratory for analysis is the most common type. (home-remedies-for-you.com)
Laboratory team performance is monitored using several techniques. (cdc.gov)
False-negatives can occur when the urine drug concentration is below the threshold level set by the laboratory performing the test. (uspharmacist.com)
A clinically compatible case in which a high index of suspicion (credible threat or patient history regarding location and time) exists for colchicine exposure, or an epidemiologic link exists between this case and a laboratory-confirmed case. (cdc.gov)

A simple, selective and sensitive method has been developed to determine debrisoquine and 4-hydroxydebrisoquine in human urine. (hacettepe.edu.tr)
Nevertheless, the simplest method of passing the urine drug test is to abstain from drug consumption. (home-remedies-for-you.com)

Urine concentration of MPB increased in a dose-dependent manner with increased frequency of use of several cosmetic products. (biomedcentral.com)
While hypercortisolism is uncommon, the signs and symptoms are common and include obesity, high blood pressure, and increased blood glucose concentration. (medscape.com)

Given the high prevalence of behaviorally active caffeine doses in the worldwide diet, significant scientific interest in the health effects of caffeine has developed. (cdc.gov)
Given the high prevalence of nonadherence, we should assess patients, particularly those on multiple antihypertensive medications or those who do not have an expected response to treatment," said co-lead author Dr Pankaj Gupta. (pharmacytimes.com)
Clinicians should be aware of the high prevalence of sleep problems among their patients with diabetes and should consider screening and treatment, which may improve patients' quality of life. (cdc.gov)

Urine drug screening is a common way to test for compliance with medications having high abuse potential. (uspharmacist.com)
By identifying medications that contribute to false-negatives and false-positives, pharmacists decrease misinterpretations from urine drug screens. (uspharmacist.com)
Urine drug screen (UDS) testing can increase workplace safety, detect drug abuse, monitor patients' compliance with prescription medications, and assess suspected drug ingestions. (uspharmacist.com)
1 Thus, these tests are commonly used in clinical practice to support decision-making on the use of high-risk medications. (uspharmacist.com)
1,4 To decrease the likelihood of misinterpretation, pharmacists can help by identifying medications at high risk for causing false-negatives and false-positives and choosing medications less likely to cause these inaccuracies. (uspharmacist.com)
1-2 While chronic marijuana use will show in the urine for weeks after heavy use, other medications and illicit drugs will only be present for 1 to 4 days, as shown in TABLE 1 . (uspharmacist.com)

and screening for fecal parasites, urine parasites, and blood microfilariae. (cdc.gov)
The clearance of D-serine was calculated based on the D-serine levels in the blood and urine that, measured using a two-dimensional high performance liquid chromatography system, which is the most accurate and sensitive system for measuring D-amino acids. (sciencedaily.com)
DSN: SEE BLOOD AND URINE DATASET NAMES ABSTRACT General Information HISPANIC HEALTH AND NUTRITION EXAMINATION SURVEY, 1982-84 Mexican Americans Cuban Americans Puerto Ricans Description The Hispanic Health and Nutrition Examination Survey (HHANES) was conducted from July 1982 through December 1984. (cdc.gov)
In addition to the blood pressure criteria, proteinuria of greater than or equal to 0.3 grams in a 24-hour urine specimen, a protein (mg/dL)/creatinine (mg/dL) ratio of 0.3 or higher, or a urine dipstick protein of 1+ (if a quantitative measurement is unavailable) is required to diagnose preeclampsia. (medscape.com)
Nursing Central , nursing.unboundmedicine.com/nursingcentral/view/Davis-Lab-and-Diagnostic-Tests/425047/all/Porphyrins_Blood_and_Urine. (unboundmedicine.com)

2,4-D is not metabolized by humans and is eliminated unchanged and rapidly in the urine. (cdc.gov)

The odds were highest for diuretics. (pharmacytimes.com)

or for other reasons are at particularly high risk of negative consequences from misinterpreted UDS results. (uspharmacist.com)
Patients may purposefully attempt to hide positive screening results by adding contaminants to their urine that mask the presence of a drug, such as vinegar, soap, bleach, drain cleaner, eye drops, table salt, or ammonia. (uspharmacist.com)

Urine test determines the urine components and wastes produced by the kidneys. (home-remedies-for-you.com)
A sample of urine is taken in a sterile test tube. (home-remedies-for-you.com)
Urine drug test is a peril, as it highlights on other parameters also. (home-remedies-for-you.com)
Urine drug test gives an idea of the prescription drugs and nonprescription drugs, such as, marijuana. (home-remedies-for-you.com)
Vitamin B2 urine color drug test is one more indicator. (home-remedies-for-you.com)
An efficient way for clearing the drug test is the powdered urine kit. (home-remedies-for-you.com)
This test was developed and its performance characteristics determined by NMS Labs. (nmslabs.com)

Four crossbred lactating Holstein Friesian cows (75% Holstein Friesian with 25% Thai native breed) with an initial body weight (BW) of 410 ± 20 kg were randomly assigned to a 2 × 2 factorial arrangement [two crude protein (CP) levels with soybean meal (SBM) or mangosteen peel liquid-protected soybean meal (MPLP)-SBM] in a 4 × 4 Latin square design experiment. (frontiersin.org)
The levels of adipo(cyto)kines in D-IBS(+) patients were higher than those of controls, and IL-6 levels correlated with those of LPS. (hindawi.com)
Leptin and BDNF were significantly higher, and neurotensin levels were significantly lower in D-IBS(+) than in controls. (hindawi.com)
In breast milk jaundice, the bilirubin may increase to levels as high as 20 mg/dL, necessitating the need for phototherapy and the discontinuation of breastfeeding. (medscape.com)
Reported frequency of use of personal care products correlated very well with urine biomarker levels of paraben and phenols. (biomedcentral.com)
The highest levels of triterpene compounds were found in the cranberry fruits harvested in the spring of the following year after the snowmelt. (bvsalud.org)
The use of principal component analysis showed that cranberry plant material harvested in October and November had higher levels of bioactive compounds. (bvsalud.org)
therefore, higher levels of cortisol are found in women, during pregnancy, and in persons on estrogen therapy. (medscape.com)

Solvent exposure from liquid sampling media. (cdc.gov)

Urine tests are cost effective and easy and forms a vital part of diagnosis. (home-remedies-for-you.com)
Diabetes was defined by self-reported diagnosis or glycohemoglobin of 6.5% or higher. (cdc.gov)

Additionally, the combinational analysis of clearances of D-serine and creatinine could measure GFR with high performance. (sciencedaily.com)

High intake of caffeine can cause sleep problems, irritability, and increased bowel activity in breastfed infants. (medlineplus.gov)

5 Changes in urine appearance, color, specific gravity, or pH may indicate the presence of a contaminant and should be checked. (uspharmacist.com)
The biomarker concentrations of bisphenol S was higher in participants with positive specific IgE and females with current asthma, but did not differ by eczema or rhinitis status. (biomedcentral.com)

Usually, a 24-hr urine collection is ordered for urine testing. (unboundmedicine.com)
Discard first morning urine then collect all urine for 24 hours, keep refrigerated throughout collection. (garcialab.com)
Alternate collection with preservative: Refrigerate urine during collection (critical) and add 25.0 mL 6N HCl to the entire collection within 4 hours of collection completion. (garcialab.com)

Mix well and send 25.0 mL urine refrigerated in a screw-capped plastic urine container. (garcialab.com)

This study investigated the use of liquid-containing phytonutrients in dairy cows as a dietary additives to reduce rumen protein degradation. (frontiersin.org)
However, the use of phytonutrient to protect protein sources and enhance protein utilization and ruminant performance has very limited information ( 10 , 11 ). (frontiersin.org)

There is a concern that high doses of caffeine might cause seizures or decrease the effects of drugs used to prevent seizures. (medlineplus.gov)

D-IBS(−) patients had a significantly higher GSRS cluster pain and diarrhoea profile than D-IBS(+) ones. (hindawi.com)

People take guarana by mouth for obesity, athletic performance, mental performance, to increase energy, and for many other reasons, but there is no good scientific evidence to support these uses. (medlineplus.gov)

Diabetes is associated with a higher risk of sleep problems, including not only sleep apnea but also inadequate sleep, excessive sleepiness, leg symptoms, and nocturia, independent of body mass index. (cdc.gov)

Chinese Dai subjects had a higher frequency of the mutant CYP2C19*2 allele (0.303) and a lower frequency of the mutant CYP2C19*3 allele (0.034). (nih.gov)
Women had higher median urine concentrations of phenolic chemicals and reported a higher frequency of use of personal care products than men. (biomedcentral.com)

The study will involve 1280 infants in a high endemic area and 2440 infants in a low endemic area, in Tanzania. (druglib.com)

The consumption of diets high in phytoestrogen-rich foods has been associated with lower rates of hormone-dependent cancers (Peeters et al. (cdc.gov)

1,2 Dilute urine, the duration of time between ingestion of the drug and time of testing, and the quantity of the drug ingested may affect the occurrence of false-negatives. (uspharmacist.com)
Identification of a drug that could be used safely and effectively for IPTi instead of SP in areas, such as north eastern Tanzania, where there is a high level of resistance to SP and amodiaquine. (druglib.com)

A wash before providing a urine sample is essential. (home-remedies-for-you.com)
It can be passed at ease by making a sample of urine from the kit. (home-remedies-for-you.com)

Anatomy11

Organisms17

Chemicals and Drugs124

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Psychiatry and Psychology2

Phenomena and Processes46

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science4

Health Care7

Mass spectrometry19

HPLC5

Metabolites14

Determination4

Erythrocyte protoporphyrin1

Solid-phase extr1

Detection3

Porphobilinogen1

Hydrolysis1

Serum3

Tandem1

Aliquot1

Metabolite1

Specimen collection1

Samples10

Biomarkers1

Quantitation2

Separation1

Specimens1

Acid2

Analytes1

Quality Assurance1

Plasma2

Bile1

19961

Laboratory5

Method2

Concentration2

Prevalence3

Medications6

Blood5

Humans1

Diuretics1

Results2

Test7

Levels8

Exposure1

Diagnosis2

Creatinine1

Intake1

Specific2

Collection3

Container1

Protein2

Caffeine1

Patients1

Obesity1

Symptoms1

Frequency2

Study1

Diets1

Drug2

Sample2