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  • dUTP
  • SYBR GreenER™ qPCR SuperMix Universal is supplied at a 2X concentration and contains hot-start Taq DNA polymerase, SYBR GreenER™ fluorescent dye, MgCl 2 , dNTPs (with dUTP instead of dTTP), UDG, and stabilizers. (thermofisher.com)
  • Mutation
  • Thus, the native Kumamolisin strand that still contained the U's from the UNG-/DUT- strain was degraded, and the new cells incorporated our desired mutation when synthesizing new DNA from the variant strand. (igem.org)
  • DNA repair is essential for parasite existence to prevent gene mutations by both spontaneous mutation during DNA replication of cell division and drug-induced mutation. (beds.ac.uk)
  • residues
  • Surprisingly, these p56 inhibitory elements are unexpectedly analogous to features used by ugi despite profound architectural disparity.Contacts from B-DNA to UDG are mimicked by residues of the p56 helix, echoing the role of ugi's inhibitory beta strand.Using mutagenesis, we propose that DNA mimicry by p56 is a targeting and specificity mechanism supporting tight inhibition via hydrophobic sequestration. (nih.gov)
  • Contacts from B-DNA to UDG are mimicked by residues of the p56 helix, echoing the role of ugi's inhibitory beta strand. (nih.gov)
  • inhibitors
  • Pentapeptide repeat proteins are therefore of wide interest not only because of their unusual structure, function, and potential as an antibacterial target, but also because knowledge of their mechanism of action may lead to both a greater understanding of the details of DNA gyrase function as well as being a useful template for the design of new DNA gyrase inhibitors. (springer.com)
  • polymerase
  • Platinum Taq DNA polymerase is recombinant Taq DNA polymerase complexed with specific monoclonal antibodies that inhibit polymerase activity at room temperature. (thermofisher.com)
  • The Taq DNA polymerase provided in the SuperMix has been chemically modified to block polymerase activity at ambient temperatures, allowing room-temperature setup and long-term storage at 4°C. Activity is restored after a 10-minute incubation in PCR cycling, providing an automatic hot start for increased sensitivity, specificity, and yiel (3). (thermofisher.com)
  • The oligo harboring the desired point mutations was then extended, using the wild-type gene and associated vector as a template, by DNA polymerase. (igem.org)
  • methylation
  • Transcription and extracellular-secretion levels of TGFBIp were high in normal cells compared with those in GCD2-derived cells and were related to H3K4me3 levels but not to DNA methylation over the TGFBI locus. (biomedcentral.com)
  • Amplification
  • SYBR GreenER™ qPCR SuperMix Universal is a ready-to-use cocktail containing all components, except primers and template, for the amplification and detection of DNA in real-time quantitative PCR (qPCR). (thermofisher.com)
  • copies
  • Note that 1 μg of plasmid DNA contains 9.1 × 10 11 copies divided by the plasmid size in kilobases. (thermofisher.com)
  • During every cell division, more than three billion DNA base pairs are replicated and copies of the genome are transferred to the daughter cells. (slideshare.net)
  • Molecular
  • The reason our genetic material does not disintegrate into complete chemical chaos is that a host of molecular systems continuously monitor and repair DNA. (slideshare.net)
  • This insight led him to discover a molecular machinery, base excision repair, which constantly counteracts the collapse of our DNA. (slideshare.net)
  • Biochemical
  • On the basis of the three‐dimensional structure and taking account of previous biochemical experiments, we propose a DNA‐binding mode and reaction mechanism for MutM. (embopress.org)
  • assay
  • Microarray and gene-expression profiling, Chromatin immunoprecipitation microarray analysis, and Methylated DNA isolation assay-assisted CpG microarrays was performed in Wild-type and GCD2-homozygous human cells. (biomedcentral.com)
  • plasmid
  • Considering transformation efficiency, stability of plasmid DNA, and growth rate, Escherichia coli and Saccharomyces cerevisiae are best suited for these experiments. (asm.org)
  • Damage
  • 2. 1 (15) The Royal Swedish Academy of Sciences has decided to award Tomas Lindahl, Paul Modrich and Aziz Sancar the Nobel Prize in Chemistry 2015 for their "Mechanistic studies of DNA repair" Damage to the genetic material poses a threat to all organisms. (slideshare.net)
  • In particular, the 8‐oxoguanine (GO) lesion is one of the most stable products of oxidative DNA damage ( Dizdaroglu, 1985 ). (embopress.org)
  • pathway
  • DNA glycosylases are the first enzymes in the BER pathway, and recognizing specific damaged bases, they act as the key enzymes to drive the BER process. (beds.ac.uk)
  • structural
  • Given that G-quadruplexes are established transcriptional regulatory elements in human [ 3 , 22 , 23 ] it is possible that the simulation of structural DNA regulatory elements of the host by the virus may help complete the virus life cycle. (biomedcentral.com)
  • whereas
  • A helix from one of the subunits of p56 occupies the UDG DNA-binding cleft, whereas the dimer interface forms a hydrophobic box to trap a mechanistically important UDG residue. (nih.gov)
  • Incubation
  • Nicking activity (pBR322 DNA): Not detectable in up to 20 U after 16 hours incubation at +37°C. (roche.com)
  • Exonucleases ( 3 H-DNA): Not detectable in up to 3 U after 4 hours incubation at +37°C. (roche.com)
  • viruses
  • Herpesviruses are ubiquitous large DNA viruses that are amongst the best known host-adapted viruses. (biomedcentral.com)
  • In addition, transcription in large DNA viruses is completely dependent on the host transcription machinery [ 20 ] and the mimicking of host-regulatory elements is an essential part of virus evolution. (biomedcentral.com)
  • chemistry
  • 1. 7 OCTOBER 2015 Scientific Background on the Nobel Prize in Chemistry 2015 MECHANISTIC STUDIES OF DNA REPAIR compiled by the Class for Chemistry of the Royal Swedish Academy of Sciences THE ROYAL SWEDISH ACADEMY OF SCIENCES, founded in 1739, is an independent organisation whose overall objective is to promote the sciences and strengthen their influence in society. (slideshare.net)
  • potential
  • Unexpectedly, the utility tracked independently of DNA-damaging treatments and instead with different tumor metastasis potential. (cancerindex.org)
  • activity
  • Inhibitory effects of 12 uracil-derivatives on PfUDG activity were investigated. (beds.ac.uk)
  • no activity on dU-free natural DNA and RNA. (roche.com)
  • Naidoo, K. J., Conformational flexibility of Sulfur Linked Saccharides a Possible Key to their Glycosidase Inhibitor Activity. (uct.ac.za)
  • stable
  • In the early 1970s, scientists believed that DNA was an extremely stable molecule, but Tomas Lindahl demonstrated that DNA decays at a rate that ought to have made the development of life on Earth impossible. (slideshare.net)
  • fluorescent
  • In qPCR, as dsDNA accumulates, the SYBR GreenER™ qPCR SuperMix dye generates a fluorescent signal that is proportional to the DNA concentration (1, 2). (thermofisher.com)
  • shift
  • We used electrophoretic mobility shift assays and found DNA binding regions between positions -134 to -103 and -255 to -215. (biomedcentral.com)
  • found
  • Studies reported in 2008, based on ¹²C/¹³C isotopic ratios of organic compounds found in the Murchison meteorite, suggested that uracil, xanthine and related molecules were formed extraterrestrially. (definitions.net)
  • system
  • In 2012 an analysis of data from the Cassini mission orbiting in the Saturn system showed that Titan's surface composition may include uracil. (definitions.net)
  • However, very little is known about malarial DNA repair system and its significance. (beds.ac.uk)
  • structure
  • Positions marked with a gold triangle show important points of contact between HHV-1 UDG and p56 observed in this study, which are also seen in contacts between distorted B-DNA with human UDG-DNA in structure 1SSP (Figure 3, and Supplementary Table S1). (nih.gov)
  • To counteract this threat, cells have evolved a series of intricate DNA repair pathways that correct DNA lesions affecting base pairing or structure of DNA. (slideshare.net)
  • cell division
  • Furthermore, defects can also arise when DNA is copied during cell division, a process that occurs several million times every day in the human body. (slideshare.net)
  • Paul Modrich has demonstrated how the cell corrects errors that occur when DNA is replicated during cell division. (slideshare.net)